Article ; Online: Bioluminescence Color-Tuning Firefly Luciferases: Engineering and Prospects for Real-Time Intracellular pH Imaging and Heavy Metal Biosensing.
2022 Volume 12, Issue 6
Abstract: Firefly luciferases catalyze the efficient production of yellow-green light under normal physiological conditions, having been extensively used for bioanalytical purposes for over 5 decades. Under acidic conditions, high temperatures and the presence of ... ...
Abstract | Firefly luciferases catalyze the efficient production of yellow-green light under normal physiological conditions, having been extensively used for bioanalytical purposes for over 5 decades. Under acidic conditions, high temperatures and the presence of heavy metals, they produce red light, a property that is called pH-sensitivity or pH-dependency. Despite the demand for physiological intracellular biosensors for pH and heavy metals, firefly luciferase pH and metal sensitivities were considered drawbacks in analytical assays. We first demonstrated that firefly luciferases and their pH and metal sensitivities can be harnessed to estimate intracellular pH variations and toxic metal concentrations through ratiometric analysis. Using |
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MeSH term(s) | Animals ; Fireflies/genetics ; Hydrogen-Ion Concentration ; Luciferases/chemistry ; Luciferases/genetics ; Luciferases, Firefly/chemistry ; Luciferases, Firefly/genetics ; Luminescent Measurements/methods ; Mammals ; Mercury ; Metals, Heavy/chemistry |
Chemical Substances | Metals, Heavy ; Luciferases (EC 1.13.12.-) ; Luciferases, Firefly (EC 1.13.12.7) ; Mercury (FXS1BY2PGL) |
Language | English |
Publishing date | 2022-06-10 |
Publishing country | Switzerland |
Document type | Journal Article ; Review |
ZDB-ID | 2662125-3 |
ISSN | 2079-6374 ; 2079-6374 |
ISSN (online) | 2079-6374 |
ISSN | 2079-6374 |
DOI | 10.3390/bios12060400 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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