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  1. Article ; Online: Expression of Recombinant PfCelTOS Antigen in the Chloroplast of Chlamydomonas reinhardtii and its Potential Use in Detection of Malaria.

    Shamriz, Shabnam / Ofoghi, Hamideh

    Molecular biotechnology

    2018  Volume 61, Issue 2, Page(s) 102–110

    Abstract: Malaria is a serious but preventable and treatable infectious disease that is found in over 100 countries around the world. Correct and rapid diagnosis of malaria infection can rescue the patient of getting sicker and reduces the risk of disease ... ...

    Abstract Malaria is a serious but preventable and treatable infectious disease that is found in over 100 countries around the world. Correct and rapid diagnosis of malaria infection can rescue the patient of getting sicker and reduces the risk of disease spreading among humans. Chlamydomonas reinhardtii chloroplast is an attractive platform for expressing malaria antigens because it is capable of folding complex proteins, including those requiring disulfide bond formation, while lack the ability to glycosylate proteins; a valuable quality of any malaria protein expression system, since the Plasmodium parasite lacks N-linked glycosylation machinery. In this study, Cell-traversal protein for ookinetes and sporozoites (CelTOS) antigen from Plasmodium falciparum was expressed in the chloroplast of C. reinhardtii and a highly sensitive and specific indirect ELISA test was developed using C. reinhardtii expressed PfCelTOS to detect malaria. Results obtained demonstrated that expressed recombinant PfCelTOS accumulates as a soluble, properly folded and functional protein within C. reinhardtii chloroplast and indirect ELISA using sera from malaria-positive donors suggested the potential use of expressed PfCelTOS as a malaria antigen for diagnosis tests.
    MeSH term(s) Antigens, Protozoan/genetics ; Antigens, Protozoan/metabolism ; Biotechnology ; Chlamydomonas reinhardtii ; Chloroplasts/genetics ; Chloroplasts/metabolism ; Enzyme-Linked Immunosorbent Assay ; Gene Expression ; Humans ; Malaria/blood ; Malaria/diagnosis ; Plants, Genetically Modified ; Plasmodium falciparum/genetics ; Plasmodium falciparum/immunology ; Protozoan Proteins/genetics ; Protozoan Proteins/metabolism ; Recombinant Proteins/genetics ; Recombinant Proteins/metabolism ; Sensitivity and Specificity ; Serologic Tests
    Chemical Substances Antigens, Protozoan ; Protozoan Proteins ; Recombinant Proteins
    Language English
    Publishing date 2018-03-23
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1193057-3
    ISSN 1559-0305 ; 1073-6085
    ISSN (online) 1559-0305
    ISSN 1073-6085
    DOI 10.1007/s12033-018-0140-1
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4031: Show issues Location:
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    ab Jg. 2022: Lesesaal (EG)

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  2. Article ; Online: Engineering the chloroplast of Chlamydomonas reinhardtii to express the recombinant PfCelTOS-Il2 antigen-adjuvant fusion protein.

    Shamriz, Shabnam / Ofoghi, Hamideh

    Journal of biotechnology

    2018  Volume 266, Page(s) 111–117

    Abstract: Malaria is an infectious disease having a large negative impact on economic growth. Vaccines are considered as a novel strategy to reduce the burden of malaria. Malaria parasite has a complex life cycle and attempts are being made to develop vaccines ... ...

    Abstract Malaria is an infectious disease having a large negative impact on economic growth. Vaccines are considered as a novel strategy to reduce the burden of malaria. Malaria parasite has a complex life cycle and attempts are being made to develop vaccines that target each stage of the life cycle. Oral vaccines seem to be more feasible to implement in poor countries, since they are relatively inexpensive, needle-free administrated, mostly stable at non-refrigerated conditions and painless. By using recombinant technology, suitable oral hosts could serve as antigen delivering vehicles in developing oral vaccines. Chlamydomonas reinhardtii offers beneficial attributes as oral recombinant protein expression platform. Moreover, C. reinhardtii chloroplast is an attractive platform for expressing malaria antigens because it is capable of folding complex proteins, including those requiring disulfide bond formation, while lacking the ability to glycosylate proteins; a valuable quality of any malaria protein expression system, since the Plasmodium parasite lacks N-linked glycosylation machinery. As a first step towards developing an oral vaccine candidate against malaria, here, we expressed a fusion protein consisting of PfCelTOS, a candidate for pre-erythrocytic and transmission-blocking vaccines, fused to human interleukin-2 (IL-2) as vaccine adjuvant in the chloroplast of C. reinhardtii. The effect of light and media on recombinant protein production and cell growth was then studied. Results demonstrated that expressed recombinant proteins accumulate as a soluble, properly folded and functional protein within algal chloroplasts. Moreover, results showed that the highest cell density can be achieved using mixotrophy mode. However, protein accumulation appears to be favored by cultivating in TAP medium in low light.
    MeSH term(s) Antigens, Protozoan/biosynthesis ; Antigens, Protozoan/genetics ; Chlamydomonas reinhardtii/genetics ; Chlamydomonas reinhardtii/metabolism ; Chloroplasts/genetics ; Chloroplasts/metabolism ; Malaria Vaccines/biosynthesis ; Malaria Vaccines/genetics ; Plasmodium falciparum/genetics
    Chemical Substances Antigens, Protozoan ; Malaria Vaccines
    Language English
    Publishing date 2018-01-20
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 843647-2
    ISSN 1873-4863 ; 0168-1656 ; 1389-0352
    ISSN (online) 1873-4863
    ISSN 0168-1656 ; 1389-0352
    DOI 10.1016/j.jbiotec.2017.12.015
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2299: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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    ab Jg. 2022: Lesesaal (EG)

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  3. Article: Engineering the chloroplast of Chlamydomonas reinhardtii to express the recombinant PfCelTOS-Il2 antigen-adjuvant fusion protein

    Shamriz, Shabnam / Hamideh Ofoghi

    Journal of biotechnology. 2018 Jan. 20, v. 266

    2018  

    Abstract: Malaria is an infectious disease having a large negative impact on economic growth. Vaccines are considered as a novel strategy to reduce the burden of malaria. Malaria parasite has a complex life cycle and attempts are being made to develop vaccines ... ...

    Abstract Malaria is an infectious disease having a large negative impact on economic growth. Vaccines are considered as a novel strategy to reduce the burden of malaria. Malaria parasite has a complex life cycle and attempts are being made to develop vaccines that target each stage of the life cycle. Oral vaccines seem to be more feasible to implement in poor countries, since they are relatively inexpensive, needle-free administrated, mostly stable at non-refrigerated conditions and painless. By using recombinant technology, suitable oral hosts could serve as antigen delivering vehicles in developing oral vaccines. Chlamydomonas reinhardtii offers beneficial attributes as oral recombinant protein expression platform. Moreover, C. reinhardtii chloroplast is an attractive platform for expressing malaria antigens because it is capable of folding complex proteins, including those requiring disulfide bond formation, while lacking the ability to glycosylate proteins; a valuable quality of any malaria protein expression system, since the Plasmodium parasite lacks N-linked glycosylation machinery. As a first step towards developing an oral vaccine candidate against malaria, here, we expressed a fusion protein consisting of PfCelTOS, a candidate for pre-erythrocytic and transmission-blocking vaccines, fused to human interleukin-2 (IL-2) as vaccine adjuvant in the chloroplast of C. reinhardtii. The effect of light and media on recombinant protein production and cell growth was then studied. Results demonstrated that expressed recombinant proteins accumulate as a soluble, properly folded and functional protein within algal chloroplasts. Moreover, results showed that the highest cell density can be achieved using mixotrophy mode. However, protein accumulation appears to be favored by cultivating in TAP medium in low light.
    Keywords algae ; antigens ; burden of disease ; cell growth ; Chlamydomonas reinhardtii ; chloroplasts ; disulfide bonds ; economic development ; edible vaccines ; engineering ; glycosylation ; hosts ; humans ; infectious diseases ; interleukin-2 ; malaria ; parasites ; Plasmodium ; recombinant proteins ; vaccine adjuvants ; vaccine development
    Language English
    Dates of publication 2018-0120
    Size p. 111-117.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 843647-2
    ISSN 1873-4863 ; 0168-1656 ; 1389-0352
    ISSN (online) 1873-4863
    ISSN 0168-1656 ; 1389-0352
    DOI 10.1016/j.jbiotec.2017.12.015
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2299: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  4. Article ; Online: Outlook in the application of Chlamydomonas reinhardtii chloroplast as a platform for recombinant protein production.

    Shamriz, Shabnam / Ofoghi, Hamideh

    Biotechnology & genetic engineering reviews

    2016  Volume 32, Issue 1-2, Page(s) 92–106

    Abstract: Microalgae, also called microphytes, are a vast group of microscopic photosynthetic organisms living in aquatic ecosystems. Microalgae have attracted the attention of biotechnology industry as a platform for extracting natural products with high ... ...

    Abstract Microalgae, also called microphytes, are a vast group of microscopic photosynthetic organisms living in aquatic ecosystems. Microalgae have attracted the attention of biotechnology industry as a platform for extracting natural products with high commercial value. During last decades, microalgae have been also used as cost-effective and easily scalable platform for the production of recombinant proteins with medical and industrial applications. Most progress in this field has been made with Chlamydomonas reinhardtii as a model organism mainly because of its simple life cycle, well-established genetics and ease of cultivation. However, due to the scarcity of existing infrastructure for commercial production and processing together with relatively low product yields, no recombinant products from C. reinhardtii have gained approval for commercial production and most of them are still in research and development. In this review, we focus on the chloroplast of C. reinhardtii as an algal recombinant expression platform and compare its advantages and disadvantages to other currently used expression systems. We then discuss the strategies for engineering the chloroplast of C. reinhardtii to produce recombinant cells and present a comprehensive overview of works that have used this platform for the expression of high-value products.
    Language English
    Publishing date 2016-04
    Publishing country England
    Document type Journal Article
    ZDB-ID 49457-4
    ISSN 2046-5556 ; 0264-8725
    ISSN (online) 2046-5556
    ISSN 0264-8725
    DOI 10.1080/02648725.2017.1307673
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

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  5. Article ; Online: Design, structure prediction and molecular dynamics simulation of a fusion construct containing malaria pre-erythrocytic vaccine candidate, PfCelTOS, and human interleukin 2 as adjuvant.

    Shamriz, Shabnam / Ofoghi, Hamideh

    BMC bioinformatics

    2016  Volume 17, Page(s) 71

    Abstract: Background: Malaria infection is still widespread in some parts of the world and threatens the lives of millions of people every year. Vaccines, especially oral vaccines are considered to be effective in reducing the burden of malaria morbidity and ... ...

    Abstract Background: Malaria infection is still widespread in some parts of the world and threatens the lives of millions of people every year. Vaccines, especially oral vaccines are considered to be effective in reducing the burden of malaria morbidity and mortality. By using recombinant technology, suitable oral hosts could serve as antigen delivering vehicles in developing oral vaccines. This study was aimed towards designing and computational analysis of a fusion protein consisting of Plasmodium falciparum cell-traversal protein for ookinetes and sporozoites (PfCelTOS) fused to human interleukin-2 (IL-2) and M cell-specific peptide ligand (Co1), as a step toward developing a vaccine candidate.
    Results: To our best knowledge, the three dimensional (3D) structure of CelTOS is not reported in protein database. Therefore, we carried out computational modeling and simulation in the hope of understanding the properties and structure of PfCelTOS. Then we fused IL-2 to PfCelTOS by a flexible linker and did in silico analysis to confirm the proper folding of each domain in the designed fusion protein. In the last step, Co1 ligand was added to the confirmed fusion structure using a rigid linker and computational analysis was performed to evaluate the final fusion construct. One structure out of five predicted by I-TASSER for PfCelTOS and fusion constructs was selected based on the highest value for C-score. Molecular dynamics (MD) simulation analysis indicated that predicted structures are stable during the simulation. Ramchandran Plot analysis of PfCelTOS and fusion constructs before and after MD simulation also represented that most residues were fallen in favorable regions.
    Conclusion: In silico study showed that Co1-(AEEEK)3- IL-2-(GGGGS)3-PfCelTOS construct has a constant structure and the selected linkers are effectively able to separate the domains. Therefore, data reported in this paper represents the first step toward developing of an oral vaccine candidate against malaria infection.
    MeSH term(s) Adjuvants, Immunologic ; Antigens, Protozoan/immunology ; Drug Design ; Erythrocytes/immunology ; Humans ; Interleukin-2/chemistry ; Interleukin-2/immunology ; Malaria/immunology ; Malaria/prevention & control ; Malaria Vaccines/administration & dosage ; Malaria Vaccines/chemistry ; Malaria Vaccines/immunology ; Molecular Dynamics Simulation ; Plasmodium falciparum/cytology ; Plasmodium falciparum/immunology ; Plasmodium falciparum/metabolism ; Protein Conformation ; Protozoan Proteins/immunology ; Recombinant Proteins/immunology ; Sporozoites/immunology
    Chemical Substances Adjuvants, Immunologic ; Antigens, Protozoan ; Interleukin-2 ; Malaria Vaccines ; Protozoan Proteins ; Recombinant Proteins
    Language English
    Publishing date 2016-02-06
    Publishing country England
    Document type Journal Article
    ZDB-ID 2041484-5
    ISSN 1471-2105 ; 1471-2105
    ISSN (online) 1471-2105
    ISSN 1471-2105
    DOI 10.1186/s12859-016-0918-8
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Outlook in the application of Chlamydomonas reinhardtii chloroplast as a platform for recombinant protein production

    Shamriz, Shabnam / Hamideh Ofoghi

    Biotechnology & genetic engineering reviews. 2016 Jan. 20, v. 32, no. 1-2

    2016  

    Abstract: Microalgae, also called microphytes, are a vast group of microscopic photosynthetic organisms living in aquatic ecosystems. Microalgae have attracted the attention of biotechnology industry as a platform for extracting natural products with high ... ...

    Abstract Microalgae, also called microphytes, are a vast group of microscopic photosynthetic organisms living in aquatic ecosystems. Microalgae have attracted the attention of biotechnology industry as a platform for extracting natural products with high commercial value. During last decades, microalgae have been also used as cost-effective and easily scalable platform for the production of recombinant proteins with medical and industrial applications. Most progress in this field has been made with Chlamydomonas reinhardtii as a model organism mainly because of its simple life cycle, well-established genetics and ease of cultivation. However, due to the scarcity of existing infrastructure for commercial production and processing together with relatively low product yields, no recombinant products from C. reinhardtii have gained approval for commercial production and most of them are still in research and development. In this review, we focus on the chloroplast of C. reinhardtii as an algal recombinant expression platform and compare its advantages and disadvantages to other currently used expression systems. We then discuss the strategies for engineering the chloroplast of C. reinhardtii to produce recombinant cells and present a comprehensive overview of works that have used this platform for the expression of high-value products.
    Keywords aquatic ecosystems ; autotrophs ; biotechnology ; Chlamydomonas reinhardtii ; chloroplasts ; cost effectiveness ; engineering ; genetics ; high-value products ; industrial applications ; infrastructure ; microalgae ; models ; recombinant proteins ; research and development
    Language English
    Dates of publication 2016-0120
    Size p. 92-106.
    Publishing place Taylor & Francis
    Document type Article
    ZDB-ID 2500039-1
    ISSN 2046-5556 ; 0264-8725
    ISSN (online) 2046-5556
    ISSN 0264-8725
    DOI 10.1080/02648725.2017.1307673
    Database NAL-Catalogue (AGRICOLA)

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  7. Article: Soluble Expression of Recombinant Nerve Growth Factor in Cytoplasm of

    Shamriz, Shabnam / Ofoghi, Hamideh / Amini-Bayat, Zahra

    Iranian journal of biotechnology

    2017  Volume 14, Issue 1, Page(s) 16–22

    Abstract: Background: Pivotal roles of Nerve growth factor (NGF) in the development and survival of both neuronal and non-neuronal cells indicate its potential for the treatment of neurodegenerative diseases. However, investigation of NGF deficits in different ... ...

    Abstract Background: Pivotal roles of Nerve growth factor (NGF) in the development and survival of both neuronal and non-neuronal cells indicate its potential for the treatment of neurodegenerative diseases. However, investigation of NGF deficits in different diseases requires the availability of properly folded human β-NGF. In previous studies bacterial expression of hNGF demonstrated the feasibility of its overproduction. However, known limitations in the use of
    Objectives: Here an optimized system was developed to overexpress the soluble NGF in
    Materials and methods: The gene encoding the β subunit of mature hNGF was optimized based on
    Results: The highest soluble expression level was achieved when
    Conclusions: Our results indicated that the recombinant NGF was successfully expressed as a soluble form.
    Language English
    Publishing date 2017-09-14
    Publishing country Iran
    Document type Journal Article
    ZDB-ID 2223669-7
    ISSN 2322-2921 ; 1728-3043
    ISSN (online) 2322-2921
    ISSN 1728-3043
    DOI 10.15171/ijb.1331
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Effect of linker length and residues on the structure and stability of a fusion protein with malaria vaccine application.

    Shamriz, Shabnam / Ofoghi, Hamideh / Moazami, Nasrin

    Computers in biology and medicine

    2016  Volume 76, Page(s) 24–29

    Abstract: Background: Recombinant protein technology has revolutionized the world of biology and medicine. Following this progress, fusion protein technology, as a novel innovation, has opened new horizons for the development of proteins that do not naturally ... ...

    Abstract Background: Recombinant protein technology has revolutionized the world of biology and medicine. Following this progress, fusion protein technology, as a novel innovation, has opened new horizons for the development of proteins that do not naturally exist. Fusion proteins are generated via genetically fusing two or more genes coding for separate proteins, thus the product is a single protein having functional properties of both proteins. As an indispensable element in fusion protein construction, linkers are used to separate the functional domains in order to improve their expression, folding and stability.
    Method: We computationally fused an antigen and an adjuvant together using different linkers to obtain a two-domain fusion construct which can potentially act as an oral vaccine candidate against malaria. We then predicted the structures computationally to find out the probable folding of each domain in the designed construct.
    Results: One of the fusion constructs was selected based on the highest value for C-score. Ramchandran Plot analysis represented that most residues were fallen in favorable regions.
    Conclusion: Our in silico analysis showed that (GGGGS)3 linker confers the best structure and stability for our target fusion protein.
    Language English
    Publishing date 2016--01
    Publishing country United States
    Document type Journal Article
    ZDB-ID 127557-4
    ISSN 1879-0534 ; 0010-4825
    ISSN (online) 1879-0534
    ISSN 0010-4825
    DOI 10.1016/j.compbiomed.2016.06.015
    Database MEDical Literature Analysis and Retrieval System OnLINE

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