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Article ; Online: Downregulation of the FTO m

Jeschke, Jana / Collignon, Evelyne / Al Wardi, Clémence / Krayem, Mohammad / Bizet, Martin / Jia, Yan / Garaud, Soizic / Wimana, Zéna / Calonne, Emilie / Hassabi, Bouchra / Morandini, Renato / Deplus, Rachel / Putmans, Pascale / Dube, Gaurav / Singh, Nitesh Kumar / Koch, Alexander / Shostak, Kateryna / Rizzotto, Lara / Ross, Robert L /

Desmedt, Christine / Bareche, Yacine / Rothé, Françoise / Lehmann-Che, Jacqueline / Duterque-Coquillaud, Martine / Leroy, Xavier / Menschaert, Gerben / Teixeira, Luis / Guo, Mingzhou / Limbach, Patrick A / Close, Pierre / Chariot, Alain / Leucci, Eleonora / Ghanem, Ghanem / Yuan, Bi-Feng / Willard-Gallo, Karen / Sotiriou, Christos / Marine, Jean-Christophe / Fuks, François

Nature cancer

2021 Volume 2, Issue 6, Page(s) 611–628

Abstract: Post-transcriptional modifications of RNA constitute an emerging regulatory layer of gene expression. The demethylase fat mass- and obesity-associated protein (FTO), an eraser of ... ...

Abstract	Post-transcriptional modifications of RNA constitute an emerging regulatory layer of gene expression. The demethylase fat mass- and obesity-associated protein (FTO), an eraser of N
MeSH term(s)	Alpha-Ketoglutarate-Dependent Dioxygenase FTO/genetics ; Animals ; Down-Regulation/genetics ; Epithelial-Mesenchymal Transition/genetics ; Humans ; Mice ; Neoplasms, Glandular and Epithelial ; RNA
Chemical Substances	RNA (63231-63-0) ; FTO protein, mouse (EC 1.14.11.-) ; Alpha-Ketoglutarate-Dependent Dioxygenase FTO (EC 1.14.11.33) ; FTO protein, human (EC 1.14.11.33)
Language	English
Publishing date	2021-06-23
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ISSN	2662-1347
ISSN (online)	2662-1347
DOI	10.1038/s43018-021-00223-7
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Article ; Online: “I felt like I was a bad person… which I’m not”

Evangeline Warren / Alexandra Kissling / Alison H. Norris / Priya R. Gursahaney / Danielle Bessett / Maria F. Gallo

SSM: Qualitative Research in Health, Vol 2, Iss , Pp 100059- (2022)

Stigmatization in crisis pregnancy centers

2022

Abstract: Stigma is present throughout everyday interactions but has particular salience for people seeking abortion care. Using Goffman’s conceptualization of stigma as a marker and enforcer of social ostracization, we expand on existing understandings of ... ...

Abstract	Stigma is present throughout everyday interactions but has particular salience for people seeking abortion care. Using Goffman’s conceptualization of stigma as a marker and enforcer of social ostracization, we expand on existing understandings of abortion stigma and its management. We draw on interviews with 12 clients and 10 staff members of crisis pregnancy centers (CPCs) in Ohio to demonstrate how stigmatization around abortion begins before an abortion occurs. We find evidence of enacted and anticipated stigmatization and document how women who are considering an abortion mitigate stigmatization through impression management and other responsive mechanisms. This project expands on existing literature by articulating the broad reality of abortion stigma and shows the concrete ways anticipated and experienced stigmatization can change an individual’s behavior in a health care setting.
Keywords	Crisis pregnancy centers ; Stigma ; Abortion stigma ; Impression management ; Public aspects of medicine ; RA1-1270
Subject code	170
Language	English
Publishing date	2022-12-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
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Article ; Online: Large-scale chemical-genetics yields new M. tuberculosis inhibitor classes.

Johnson, Eachan O / LaVerriere, Emily / Office, Emma / Stanley, Mary / Meyer, Elisabeth / Kawate, Tomohiko / Gomez, James E / Audette, Rebecca E / Bandyopadhyay, Nirmalya / Betancourt, Natalia / Delano, Kayla / Da Silva, Israel / Davis, Joshua / Gallo, Christina / Gardner, Michelle / Golas, Aaron J / Guinn, Kristine M / Kennedy, Sofia / Korn, Rebecca /

McConnell, Jennifer A / Moss, Caitlin E / Murphy, Kenan C / Nietupski, Raymond M / Papavinasasundaram, Kadamba G / Pinkham, Jessica T / Pino, Paula A / Proulx, Megan K / Ruecker, Nadine / Song, Naomi / Thompson, Matthew / Trujillo, Carolina / Wakabayashi, Shoko / Wallach, Joshua B / Watson, Christopher / Ioerger, Thomas R / Lander, Eric S / Hubbard, Brian K / Serrano-Wu, Michael H / Ehrt, Sabine / Fitzgerald, Michael / Rubin, Eric J / Sassetti, Christopher M / Schnappinger, Dirk / Hung, Deborah T

Nature

2019 Volume 571, Issue 7763, Page(s) 72–78

Abstract: New antibiotics are needed to combat rising levels of resistance, with new Mycobacterium tuberculosis (Mtb) drugs having the highest priority. However, conventional whole-cell and biochemical antibiotic screens have failed. Here we develop a strategy ... ...

Abstract	New antibiotics are needed to combat rising levels of resistance, with new Mycobacterium tuberculosis (Mtb) drugs having the highest priority. However, conventional whole-cell and biochemical antibiotic screens have failed. Here we develop a strategy termed PROSPECT (primary screening of strains to prioritize expanded chemistry and targets), in which we screen compounds against pools of strains depleted of essential bacterial targets. We engineered strains that target 474 essential Mtb genes and screened pools of 100-150 strains against activity-enriched and unbiased compound libraries, probing more than 8.5 million chemical-genetic interactions. Primary screens identified over tenfold more hits than screening wild-type Mtb alone, with chemical-genetic interactions providing immediate, direct target insights. We identified over 40 compounds that target DNA gyrase, the cell wall, tryptophan, folate biosynthesis and RNA polymerase, as well as inhibitors that target EfpA. Chemical optimization yielded EfpA inhibitors with potent wild-type activity, thus demonstrating the ability of PROSPECT to yield inhibitors against targets that would have eluded conventional drug discovery.
MeSH term(s)	Antitubercular Agents/classification ; Antitubercular Agents/isolation & purification ; Antitubercular Agents/pharmacology ; DNA Gyrase/metabolism ; Drug Discovery/methods ; Drug Resistance, Microbial ; Folic Acid/biosynthesis ; Gene Deletion ; Microbial Sensitivity Tests/methods ; Molecular Targeted Therapy ; Mycobacterium tuberculosis/cytology ; Mycobacterium tuberculosis/drug effects ; Mycobacterium tuberculosis/enzymology ; Mycobacterium tuberculosis/genetics ; Mycolic Acids/metabolism ; Reproducibility of Results ; Small Molecule Libraries/classification ; Small Molecule Libraries/isolation & purification ; Small Molecule Libraries/pharmacology ; Substrate Specificity ; Topoisomerase II Inhibitors/isolation & purification ; Topoisomerase II Inhibitors/pharmacology ; Tryptophan/biosynthesis ; Tuberculosis/drug therapy ; Tuberculosis/microbiology
Chemical Substances	Antitubercular Agents ; Mycolic Acids ; Small Molecule Libraries ; Topoisomerase II Inhibitors ; Tryptophan (8DUH1N11BX) ; Folic Acid (935E97BOY8) ; DNA Gyrase (EC 5.99.1.3)
Language	English
Publishing date	2019-06-19
Publishing country	England
Document type	Journal Article
ZDB-ID	120714-3
ISSN	1476-4687 ; 0028-0836
ISSN (online)	1476-4687
ISSN	0028-0836
DOI	10.1038/s41586-019-1315-z
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Zs.A 26: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
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Article ; Online: Microfragmented Adipose Tissue (M-FATS) for Improved Healing of Surgically Repaired Achilles Tendon Tears: A Preliminary Study.

Ferracini, Riccardo / Artiaco, Stefano / Daghino, Walter / Falco, Mara / Gallo, Alessandra / Garibaldi, Riccardo / Tiraboschi, Emilia / Guidotti, Claudio / Bistolfi, Alessandro

Foot & ankle specialist

2020 Volume 15, Issue 5, Page(s) 472–478

Abstract: ... of the adjunct of microfragmented adipose tissue (M-FATS) after the suture of a series of Achilles tendons ... with perilesional application of a preparation of M-FATS rich in mesenchymal stem cells. Results were compared ... groups. US evaluation showed quicker tendon remodeling in the M-FATS group. Adverse events were not ...

Abstract	Introduction: Tendon healing is a complicated process that results in inferior structural and functional properties when compared with healthy tendon; the purpose of this study was to assess the effects of the adjunct of microfragmented adipose tissue (M-FATS) after the suture of a series of Achilles tendons. Methods: After complete Achilles tendon tear, 8 patients underwent open suture repair in conjunction with perilesional application of a preparation of M-FATS rich in mesenchymal stem cells. Results were compared with a similar group of patients treated with conventional open suture. Outcomes were evaluated based on range of motion, functional recovery, and complications according to the American Orthopedic Foot and Ankle Society (AOFAS) score and Foot and Ankle Disability Index (FADI). Achilles tendons were examined by ultrasound (US) at 3 months. Results: The AOFAS and FADI scores showed no differences between the 2 groups. US evaluation showed quicker tendon remodeling in the M-FATS group. Adverse events were not documented for both procedures. Conclusions: The combined application of derived M-FATS for tendon rupture is safe and presents new possibilities for enhanced healing. Levels of evidence: Level IIIb: Case control study.
MeSH term(s)	Achilles Tendon/surgery ; Adipose Tissue ; Case-Control Studies ; Humans ; Rupture/surgery ; Treatment Outcome
Language	English
Publishing date	2020-11-26
Publishing country	United States
Document type	Journal Article
ZDB-ID	2488579-4
ISSN	1938-7636 ; 1938-6400
ISSN (online)	1938-7636
ISSN	1938-6400
DOI	10.1177/1938640020974557
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Zs.A 7037: Show issues

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Article: Hodnocení výsledků náhrady předního zkříženého vazu pomocí alogenního štěpu ze šlachy m. tibialis anterior.

Kozák, M / Gallo, J / Langová, K / Holinka, M

Acta chirurgiae orthopaedicae et traumatologiae Cechoslovaca

2013 Volume 80, Issue 1, Page(s) 47–52

Abstract: Purpose of the study: Injury to the anterior cruciate ligament (ACL) frequently results in the development of knee instability. This is managed by ACL reconstruction using autogenous or allogeneic grafts. This study presents a comprehensive evaluation ... ...

Title translation	Evaluation of anterior cruciate ligament reconstruction using anterior tibial muscle tendon allograft.
Abstract	Purpose of the study: Injury to the anterior cruciate ligament (ACL) frequently results in the development of knee instability. This is managed by ACL reconstruction using autogenous or allogeneic grafts. This study presents a comprehensive evaluation of the patients with ACL reconstruction using a tendon allograft harvested from the anterior tibial muscle (ATM). Material and methods: The group under study comprised 53 patients, 33 men and 20 women, with unilateral ACL reconstruction while the other knee was intact. The average age was 32.4 years (17 to 51) and the average follow-up was 33,4 months (21 to 53). Clinical evaluation included the Tegner and Lysholm scores and questionnaire-based satisfaction rating. Functional assessment included measurements of the range of motion and thigh circumference. Knee stability was determined using the pivot shift test, Lachman test and one-leg hop test. ACL laxity was measured on a GNRB® arthrometer. Placement of the tibial and the femoral tunnel on radiographs was assessed by the method of Harner. Results: All patients reported their willingness to undergo the surgery again. The average pre-operative Tegner score improved from 7.17 (4-10) to 6.45 (2-10; p = 0.0001). The average post-operative Lysholm score was 87 points (47-100). At final follow-up, the full range of motion was recorded in 39 patients (74%), while the remaining patients exhibited only insignificant restriction of knee motion in comparison with the pre-operative status. The results of the Lachman test were negative in 38 patients (71.7%) while the pivot shift test was negative in 52 patients (98.1%). In the one-leg hop test, 44 patients (83%) were able to jump on the treated leg for a distance longer than 90% of the distance done on the healthy leg. The GNRB test at a force of 134N showed an anterior shift of the proximal tibia by 2.2 mm (0.1-9.3) on the average. The tibial tunnel was placed in zone B in 48 patients (90.6%) and the femoral tunnel was most often located to zone D (n = 47; 88.7%). Discussion: The choice of tissue for ACL reconstruction is still being discussed. In some centres an ATM tendon allograft is used only in revision surgery while, in other centres, it is also employed in primary procedures. Recent studies on ACL surgery suggest that outcomes are relatively independent of the material used for reconstruction. In view of evidence-based medicine, more well-conducted multicentre clinical trials are needed to optimise indications for allograft ACL reconstruction. Conclusions: The ACL reconstruction using an ATM tendon allograft showed good clinical outcome at a minimum follow-up of 21 months. The method can be recommended not only for revision ACL reconstruction but also in primary reconstructions particularly in elderly patients. The present-day technology of producing allografts maintains tissue biomechanical characteristics and reduces risks for transmission of infection to a minimum.
MeSH term(s)	Adult ; Anterior Cruciate Ligament/surgery ; Anterior Cruciate Ligament Injuries ; Anterior Cruciate Ligament Reconstruction/adverse effects ; Anterior Cruciate Ligament Reconstruction/methods ; Czech Republic ; Female ; Humans ; Knee Joint/diagnostic imaging ; Knee Joint/physiopathology ; Knee Joint/surgery ; Male ; Muscle, Skeletal ; Radiography ; Range of Motion, Articular ; Recovery of Function ; Retrospective Studies ; Tendons/transplantation ; Transplantation, Autologous/adverse effects ; Transplantation, Autologous/methods ; Treatment Outcome
Language	Czech
Publishing date	2013
Publishing country	Czech Republic
Document type	Journal Article
ZDB-ID	418889-5
ISSN	0001-5415
ISSN	0001-5415
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Article: Proč selhávají artroskopické stabilizační operace na ramenn??m kloubu.

Neoral, P / Holibka, R / Kalina, R / Mlčůchová, D / Gallo, J

Acta chirurgiae orthopaedicae et traumatologiae Cechoslovaca

2014 Volume 81, Issue 1, Page(s) 51–56

Abstract: Purpose of the study: Shoulder instability adversely affects the quality of life and restricts the functional capacity of the upper extremity involved. Today stabilisation surgery is almost always performed arthroscopically. The aim of this study was to ...

Title translation	Arthroscopic stabilisation of the shoulder. Risk factors for its failure.
Abstract	Purpose of the study: Shoulder instability adversely affects the quality of life and restricts the functional capacity of the upper extremity involved. Today stabilisation surgery is almost always performed arthroscopically. The aim of this study was to present our experience with arthroscopic stabilisation of the shoulder and to identify risk factors responsible for its failure. Material and methods: The group of 110 patients with recurrent anterior dislocation of the shoulder, who were treated between January 2007 and December 2010, consisted of 19 women and 91 men with an average age of 27 years (range, 14 to 56) at the time of surgery. Patients who had a concomitant tear of the rotator cuff or of the long head tendon of the biceps were excluded from the study. The minimum follow-up period was 2 years. The patients were evaluated for signs of clinical instability. Function was evaluated using WOSI, Constant-Murley and Rowe-Zarins. Statistical methods were used to assess factors leading to failed arthroscopic stabilisation. Results: At final follow-up, 17 patients (17 shoulders, 15.3%) had re-dislocation or subluxation of the joint treated. Age over 20 years reduced the probability of re-dislocation (odds ratio, OR=0.87; p=0.021) while the finding of an engaging Hill-Sachs lesion increased the risk of re-dislocation (OR=5.53; p=0.0028). The presence of a bony Bankart lesion had only a marginal effect on the probability of re-dislocation (p=0.0512). In stable shoulders the average pre-operative values improved to the final follow-up values as follows: WOSI index, 53.65 ± 5.6 to 94.8 ± 5.2 (p<0.0001); Constant-Murley score, 78.9 ± 6.1 to 95.6 ± 4.4 (p<0.0001); and Rowe-Zarins score, 53.3 ± 6.7 to 92.9 ± 7.8 (p<0.0001). No peri-operative complication was recorded. Discussion: Our study confirmed the role of 3D CT examination before arthroscopic stabilisation of the shoulder joint. The exact identification of bone injury and its extent is of prognostic importance. At the same time special attention should be paid to surgical and post-operative tactics in patients operated on before their 20 years of age. On the other hand, no risks were found to be associated with gender, sports activities, the number of previous dislocations, types of anchors, suture material or knot tying. Conclusions: Arthroscopic stabilisation of the shoulder significantly relieves pain and improves shoulder function in 85% of the patients with anterior shoulder dislocation. The risk that this stabilisation surgery will fail increases with lower age of the patient and the presence of an engaging Hill-Sachs lesion.
MeSH term(s)	Adult ; Arthroscopy/adverse effects ; Arthroscopy/methods ; Female ; Follow-Up Studies ; Humans ; Joint Instability/diagnosis ; Joint Instability/etiology ; Joint Instability/physiopathology ; Joint Instability/psychology ; Joint Instability/surgery ; Male ; Outcome Assessment (Health Care) ; Quality of Life ; Range of Motion, Articular ; Recovery of Function ; Recurrence ; Reoperation/methods ; Risk Factors ; Shoulder Dislocation/diagnosis ; Shoulder Dislocation/etiology ; Shoulder Dislocation/physiopathology ; Shoulder Dislocation/psychology ; Shoulder Dislocation/surgery ; Shoulder Joint/injuries ; Shoulder Joint/surgery
Language	Czech
Publishing date	2014
Publishing country	Czech Republic
Document type	English Abstract ; Journal Article
ZDB-ID	418889-5
ISSN	0001-5415
ISSN	0001-5415
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Article ; Online: Identification and biochemical characterization of the novel mutation m.8839G>C in the mitochondrial ATP6 gene associated with NARP syndrome.

Blanco-Grau, A / Bonaventura-Ibars, I / Coll-Cantí, J / Melià, M J / Martinez, R / Martínez-Gallo, M / Andreu, A L / Pinós, T / García-Arumí, E

Genes, brain, and behavior

2013 Volume 12, Issue 8, Page(s) 812–820

Abstract: ... in whom a previously undescribed mutation was detected in the ATP6 gene: m.8839G>C. Several observations support ... the concept that m.8839G>C is pathogenically involved in the clinical phenotype of this patient: (1 ... that m.8839G>C may lower the efficiency between proton translocation within F0 and F1 rotation, required ...

Abstract	Mutations in the ATP6 gene are reported to be associated with Leber hereditary optic neuropathy, bilateral striatal necrosis, coronary atherosclerosis risk and neuropathy, ataxia and retinitis pigmentosa (NARP)/maternally inherited Leigh syndromes. Here, we present a patient with NARP syndrome, in whom a previously undescribed mutation was detected in the ATP6 gene: m.8839G>C. Several observations support the concept that m.8839G>C is pathogenically involved in the clinical phenotype of this patient: (1) the mutation was heteroplasmic in muscle; (2) mutation load was higher in the symptomatic patient than in the asymptomatic carriers; (3) cybrids carrying this mutation presented lower cell proliferation, increased mitochondrial DNA (mtDNA) copy number, increased steady-state OxPhos protein levels and decreased mitochondrial membrane potential with respect to isogenic wild-type cybrids; (4) this change was not observed in 2959 human mtDNAs from different mitochondrial haplogroups; (5) the affected amino acid was conserved in all the ATP6 sequences analyzed; and (6) using in silico prediction, the mutation was classified as 'probably damaging'. However, measurement of ATP synthesis showed no differences between wild-type and mutated cybrids. Thus, we suggest that m.8839G>C may lower the efficiency between proton translocation within F0 and F1 rotation, required for ATP synthesis. Further experiments are needed to fully characterize the molecular mechanisms involved in m.8839G>C pathogenicity.
MeSH term(s)	Adenosine Triphosphate/biosynthesis ; Cell Line, Tumor ; Cell Proliferation ; DNA, Mitochondrial/genetics ; Female ; Gene Dosage ; Haplotypes ; Heterozygote ; Humans ; Middle Aged ; Mitochondrial Myopathies/diagnosis ; Mitochondrial Myopathies/genetics ; Mitochondrial Myopathies/metabolism ; Mitochondrial Proteins/genetics ; Mitochondrial Proteins/metabolism ; Mitochondrial Proton-Translocating ATPases/genetics ; Mutation, Missense ; Oxidative Phosphorylation ; Retinitis Pigmentosa/diagnosis ; Retinitis Pigmentosa/genetics ; Retinitis Pigmentosa/metabolism
Chemical Substances	DNA, Mitochondrial ; MT-ATP6 protein, human ; Mitochondrial Proteins ; Adenosine Triphosphate (8L70Q75FXE) ; Mitochondrial Proton-Translocating ATPases (EC 3.6.3.-)
Language	English
Publishing date	2013-10-25
Publishing country	England
Document type	Case Reports ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2075819-4
ISSN	1601-183X ; 1601-1848
ISSN (online)	1601-183X
ISSN	1601-1848
DOI	10.1111/gbb.12089
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Zs.A 5642: Show issues

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Article ; Online: Co-exposure to n-TiO2 and Cd2+ results in interactive effects on biomarker responses but not in increased toxicity in the marine bivalve M. galloprovincialis.

Balbi, Teresa / Smerilli, Arianna / Fabbri, Rita / Ciacci, Caterina / Montagna, Michele / Grasselli, Elena / Brunelli, Andrea / Pojana, Giulio / Marcomini, Antonio / Gallo, Gabriella / Canesi, Laura

The Science of the total environment

2014 Volume 493, Page(s) 355–364

Abstract: ... did not result in increased adverse effects in M. galloprovincialis. These data underline the need ...

Abstract	The increasing production of nanoparticles (NPs) will lead to their release into the aquatic environment, where they could modify the bioavailability/bioconcentration and consequent biological impact of other contaminants. Interactive effects of n-TiO2, one of the most widespread NP type, and Cd(2+), a common heavy metal pollutant, have been described in freshwater species, whereas no information is available in marine organisms. In this work, the effects of co-exposure to n-TiO2 and Cd(2+) were investigated in the marine bivalve Mytilus galloprovincialis. Experimental conditions (100 μg/L, 96 h), were chosen in order to induce early but measurable stress responses (biomarkers) without toxicity. Several biomarkers, from molecular to tissue level, were measured in hemolymph and digestive gland; the effects on embryo development were also evaluated. In hemolymph, Cd(2+) abolished the increase in immune parameters induced by n-TiO2 (NO production and lysozyme activity). In the digestive gland, distinct interactive effects of n-TiO2 and Cd(2+) were observed on different lysosomal biomarkers (lysosomal membrane stability, lipid accumulation and lysosome/cytoplasm volume ratio) and transcription of the immune genes lysozyme and toll-like receptor (TLR). However, n-TiO2 did not affect specific metal-induced responses (metallothionein induction) and tissue metal accumulation. Cd(2+) alone, but not in combination with n-TiO2, affected embryo development. The interactive effects observed on different biomarkers were not apparently due to differences in bioavailability/bioaccumulation of Cd(2+) in the presence of n-TiO2 agglomerates; these effects may result from interactions of either contaminant with both common and distinct targets/mechanisms of action at different levels of biological organization. Overall, the results indicate that co-exposure to n-TiO2 and Cd(2+) did not result in increased adverse effects in M. galloprovincialis. These data underline the need for further knowledge on the potential interactions of NPs with existing contaminants in marine organisms.
MeSH term(s)	Animals ; Biomarkers/metabolism ; Cadmium/toxicity ; Mytilus/physiology ; Titanium/toxicity ; Water Pollutants, Chemical/toxicity
Chemical Substances	Biomarkers ; Water Pollutants, Chemical ; Cadmium (00BH33GNGH) ; titanium dioxide (15FIX9V2JP) ; Titanium (D1JT611TNE)
Language	English
Publishing date	2014-09-15
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	121506-1
ISSN	1879-1026 ; 0048-9697
ISSN (online)	1879-1026
ISSN	0048-9697
DOI	10.1016/j.scitotenv.2014.05.146
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Article ; Online: M-CHAT Mexican Version Validity and Reliability and Some Cultural Considerations.

Albores-Gallo, Lilia / Roldán-Ceballos, Ofelia / Villarreal-Valdes, Gabriela / Betanzos-Cruz, Blanca Xochitl / Santos-Sánchez, Claudia / Martínez-Jaime, Maria Magdalena / Lemus-Espinosa, Isaac / Hilton, Claudia List

ISRN neurology

2012 Volume 2012, Page(s) 408694

Abstract: The Modified Checklist for Autism in Toddlers (M-CHAT) questionnaire is a brief measure available ... CHAT-version. The study sample consisted of 456 children (age M = 4.46, SD = 1.12), 74.34% TD children ... higher for the ASD group compared with the TD group. Internal consistency for the Mexican/M-CHAT version ...

Abstract	The Modified Checklist for Autism in Toddlers (M-CHAT) questionnaire is a brief measure available in Spanish which needs to be validated for the Mexican population. Parents of children from (1) community with typical development (TD) and (2) psychiatric outpatient unit completed the CBCL/1.5-5 and the Mexican/MM-CHAT-version. The study sample consisted of 456 children (age M = 4.46, SD = 1.12), 74.34% TD children and 26.65% with Autism Spectrum Disorders (ASD). The MM-CHAT mean score for failed key items was higher for the ASD group compared with the TD group. Internal consistency for the Mexican/M-CHAT version was .76 for total score and .70 for the 6 critical items. Correlations between the MM-CHAT and the CBCL/1.5: PDD and Withdrawn subscales and with ADI-R dimensions: B non verbal) and A were high, and were moderate with ADI-R dimensions B1 (verbal) and C The failure rate of the MM-CHAT between the groups did not reproduce all the critical items found in other studies. Although the instrument has good psychometric properties and can be used for screening purposes in primary settings or busy specialized psychiatric clinics, these results support evidence for cultural differences in item responses, making it difficult to compare M-CHAT results internationally.
Language	English
Publishing date	2012-07-01
Publishing country	Egypt
Document type	Journal Article
ZDB-ID	2612992-9
ISSN	2090-5513 ; 2090-5513
ISSN (online)	2090-5513
ISSN	2090-5513
DOI	10.5402/2012/408694
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Article ; Online: Loss of nephrin expression in glomeruli of kidney-transplanted patients under m-TOR inhibitor therapy.

Biancone, L / Bussolati, B / Mazzucco, G / Barreca, A / Gallo, E / Rossetti, M / Messina, M / Nuschak, B / Fop, F / Medica, D / Cantaluppi, V / Camussi, G / Segoloni, G P

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

2010 Volume 10, Issue 10, Page(s) 2270–2278

Abstract: The development of proteinuria has been observed in kidney-transplanted patients on m-TOR inhibitor ... m-TORi) treatment. Recent studies suggest that m-TORi(s) may alter the behavior and integrity ... novo' m-TORi-treatment, the expression of nephrin within glomeruli was significantly reduced ...

Abstract	The development of proteinuria has been observed in kidney-transplanted patients on m-TOR inhibitor (m-TORi) treatment. Recent studies suggest that m-TORi(s) may alter the behavior and integrity of glomerular podocytes. We analyzed renal biopsies from kidney-transplanted patients and evaluated the expression of nephrin, a critical component of the glomerular slit-diaphragm. In a group of patients on 'de novo' m-TORi-treatment, the expression of nephrin within glomeruli was significantly reduced in all cases compared to pretransplant donor biopsies. Biopsies from control transplant patients not treated with m-TORi(s) failed to present a loss of nephrin. In a group of patients subsequently converted to m-TORi-treatment, a protocol biopsy performed before introduction of m-TORi was also available. The expression of nephrin in the pre-m-TORi biopsies was similar to that observed in the pretransplant donor biopsies but was significantly reduced after introduction of m-TORi(s). Proteinuria increased after the m-TORi inititiation in this group. However, in some cases proteinuria remained normal despite reduction of nephrin. In vitro, sirolimus downregulated nephrin expression by human podocytes. Our results suggest that m-TORi(s) may affect nephrin expression in kidney-transplanted patients, consistently with the observation in vitro on cultured podocytes.
MeSH term(s)	Adult ; Aged ; Cells, Cultured ; Humans ; Kidney Glomerulus/metabolism ; Kidney Transplantation/adverse effects ; Membrane Proteins/biosynthesis ; Middle Aged ; Podocytes/metabolism ; Proteinuria/chemically induced ; Retrospective Studies ; Sirolimus/adverse effects ; TOR Serine-Threonine Kinases/antagonists & inhibitors
Chemical Substances	Membrane Proteins ; nephrin ; MTOR protein, human (EC 2.7.1.1) ; TOR Serine-Threonine Kinases (EC 2.7.11.1) ; Sirolimus (W36ZG6FT64)
Language	English
Publishing date	2010-09-14
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2060594-8
ISSN	1600-6143 ; 1600-6135
ISSN (online)	1600-6143
ISSN	1600-6135
DOI	10.1111/j.1600-6143.2010.03259.x
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