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Article ; Online: Perspectives and experiences of the first geriatricians trained in Canada.

Wong, Eric Kai-Chung / Day, Alexander / Zorzitto, Maria / Sale, Joanna E M

2023 Volume 18, Issue 7, Page(s) e0287857

Abstract: Many Canadian-trained geriatricians from the subspecialty's first decade of existence continue to practice today. The objective of this study was to examine the experiences and perspectives of the earliest cohort of geriatricians in Canada. Using ... ...

Abstract	Many Canadian-trained geriatricians from the subspecialty's first decade of existence continue to practice today. The objective of this study was to examine the experiences and perspectives of the earliest cohort of geriatricians in Canada. Using qualitative description method, we conducted semi-structured interviews to explore participants' experiences in training and practice. We included geriatricians who trained in Canada between 1980-1989 and were in active clinical practice as of October 2021. Each transcript was coded independently by two investigators. Thematic analysis was used to develop key themes. Fourteen participants (43% female, mean years in practice 35.9) described their choice to enter geriatric medicine, their training process, the roles of a geriatrician, challenges facing the profession and advice for trainees. Two themes were developed from the data: (i) advocacy for the older adult and (ii) geriatrics as "the road less taken". Advocacy was described as the "core mission" of a geriatrician. Participants discussed the importance of advocacy in clinical practice, education, research and disseminating geriatric principles in the health system and society. "The road less taken" reflected the challenges participants faced during training, which led to relatively few geriatricians for the growing number of older adults in Canada. Despite these challenges, participants described rewarding careers and encouraged trainees to consider the profession.
MeSH term(s)	Humans ; Female ; Aged ; Male ; Geriatricians/education ; Canada ; Geriatrics/methods
Language	English
Publishing date	2023-07-06
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2267670-3
ISSN	1932-6203 ; 1932-6203
ISSN (online)	1932-6203
ISSN	1932-6203
DOI	10.1371/journal.pone.0287857
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Article ; Online: Perspectives and experiences of the first geriatricians trained in Canada.

Eric Kai-Chung Wong / Alexander Day / Maria Zorzitto / Joanna E M Sale

PLoS ONE, Vol 18, Iss 7, p e

2023 Volume 0287857

Abstract	Many Canadian-trained geriatricians from the subspecialty's first decade of existence continue to practice today. The objective of this study was to examine the experiences and perspectives of the earliest cohort of geriatricians in Canada. Using qualitative description method, we conducted semi-structured interviews to explore participants' experiences in training and practice. We included geriatricians who trained in Canada between 1980-1989 and were in active clinical practice as of October 2021. Each transcript was coded independently by two investigators. Thematic analysis was used to develop key themes. Fourteen participants (43% female, mean years in practice 35.9) described their choice to enter geriatric medicine, their training process, the roles of a geriatrician, challenges facing the profession and advice for trainees. Two themes were developed from the data: (i) advocacy for the older adult and (ii) geriatrics as "the road less taken". Advocacy was described as the "core mission" of a geriatrician. Participants discussed the importance of advocacy in clinical practice, education, research and disseminating geriatric principles in the health system and society. "The road less taken" reflected the challenges participants faced during training, which led to relatively few geriatricians for the growing number of older adults in Canada. Despite these challenges, participants described rewarding careers and encouraged trainees to consider the profession.
Keywords	Medicine ; R ; Science ; Q
Language	English
Publishing date	2023-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
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Article ; Online: Ceragenin-coated endotracheal tubes for the reduction of ventilator-associated pneumonia: a prospective, longitudinal, cross-over, interrupted time, implementation study protocol (CEASE VAP study).

Symonds, Nicola E / Meng, Eric X M / Boyd, John Gordon / Boyd, Tracy / Day, Andrew / Hobbs, Hailey / Maslove, David M / Norman, Patrick A / Semrau, Joanna S / Sibley, Stephanie / Muscedere, John

BMJ open

2024 Volume 14, Issue 2, Page(s) e076720

Abstract: Background: Critically ill patients are at high risk of acquiring ventilator-associated pneumonia (VAP), which occurs in approximately 20% of mechanically ventilated patients. VAP results either from aspiration of pathogen-contaminated oropharyngeal ... ...

Abstract	Background: Critically ill patients are at high risk of acquiring ventilator-associated pneumonia (VAP), which occurs in approximately 20% of mechanically ventilated patients. VAP results either from aspiration of pathogen-contaminated oropharyngeal secretions or contaminated biofilms that form on endotracheal tubes (ETTs) after intubation. VAP results in increased duration of mechanical ventilation, increased intensive care unit and hospital length of stay, increased risk of death and increased healthcare costs. Because of its impact on patient outcomes and the healthcare system, VAP is regarded as an important patient safety issue and there is an urgent need for better evidence on the efficacy of prevention strategies. Modified ETTs that reduce aspiration of oropharyngeal secretions with subglottic secretion drainage or reduce the occurrence of biofilm with a coating of ceragenins (CSAs) are available for clinical use in Canada. In this implementation study, we will evaluate the efficacy of these two types of Health Canada-licensed ETTs on the occurrence of VAP, and impact on patient-centred outcomes. Methods: In this ongoing, pragmatic, prospective, longitudinal, interrupted time, cross-over implementation study, we will compare the efficacy of a CSA-coated ETT (CeraShield N8 Pharma) with an ETT with subglottic secretion drainage (Taper Guard, Covidien). The study periods consist of four alternating time periods of 11 or 12 weeks or a total of 23 weeks for each ETT. All patients intubated with the study ETT in each time period will be included in an intention-to-treat analysis. Outcomes will include VAP incidence, mortality and health services utilisation including antibiotic use and length of stay. Ethics and dissemination: This study has been approved by the Health Sciences Research Ethics Board at Queen's University. The results of this study will be actively disseminated through manuscript publication and conference presentations. Trial registration number: NCT05761613.
MeSH term(s)	Humans ; Intubation, Intratracheal ; Pneumonia, Ventilator-Associated/epidemiology ; Pneumonia, Ventilator-Associated/prevention & control ; Prospective Studies ; Respiration, Artificial/adverse effects ; Steroids/therapeutic use ; Cross-Over Studies
Chemical Substances	ceragenins ; Steroids
Language	English
Publishing date	2024-02-02
Publishing country	England
Document type	Clinical Trial Protocol ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2599832-8
ISSN	2044-6055 ; 2044-6055
ISSN (online)	2044-6055
ISSN	2044-6055
DOI	10.1136/bmjopen-2023-076720
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Article ; Online: Expression of the Bacillus subtilis TasA signal peptide leads to cell death in Escherichia coli due to inefficient cleavage by LepB.

Musik, Joanna E / Zalucki, Yaramah M / Day, Christopher J / Jennings, Michael P

Biochimica et biophysica acta. Biomembranes

2021 Volume 1863, Issue 12, Page(s) 183768

Abstract: Bacillus subtilis has five type I signal peptidases, one of these, SipW, is an archaeal-like peptidase. SipW is expressed in an operon (tapA-sipW-tasA) and is responsible for removing the signal peptide from two proteins: TapA and TasA. It is unclear ... ...

Abstract	Bacillus subtilis has five type I signal peptidases, one of these, SipW, is an archaeal-like peptidase. SipW is expressed in an operon (tapA-sipW-tasA) and is responsible for removing the signal peptide from two proteins: TapA and TasA. It is unclear from the signal peptide sequence of TasA and TapA, why an archaeal-like signal peptidase is required for their processing. Bioinformatic analysis of TasA and TapA indicates that both contain highly similar signal peptide cleavage sites, both predicted to be cleaved by Escherichia coli signal peptidase I, LepB. We show that expressing full length TasA in E. coli is toxic and leads to cell death. To determine if this phenotype is due to the inability of the E. coli LepB to process the TasA signal peptide, we fused the TasA signal peptide and two amino acids of mature TasA (up to P2') to both maltose binding protein (MBP) and β-lactamase (Bla). We observed a defect in secretion, indicated by an abundance of unprocessed protein with both TasA-MBP and TasA-Bla fusions. A series of mutations in both TasA-MBP and TasA-Bla were made around the junction of the TasA signal peptide and the fusion protein. Both of these studies indicate that residues around the predicted TasA signal sequence cleavage site, particularly the sequence from P3 to P2', inhibit processing by LepB. The cell death observed when TasA and TasA signal sequence fusion proteins are expressed is likely due to the TasA signal peptide blocking LepB and thereby the general secretion pathway.
MeSH term(s)	Bacillus subtilis/drug effects ; Bacillus subtilis/pathogenicity ; Bacterial Proteins/chemistry ; Bacterial Proteins/genetics ; Cell Death/drug effects ; Escherichia coli/drug effects ; Escherichia coli/genetics ; Gene Expression Regulation, Bacterial/drug effects ; Maltose-Binding Proteins/chemistry ; Maltose-Binding Proteins/genetics ; Membrane Proteins/chemistry ; Membrane Proteins/genetics ; Mutation/genetics ; Oligopeptides/chemistry ; Oligopeptides/genetics ; Protein Binding ; Protein Sorting Signals/genetics ; Serine Endopeptidases/chemistry ; Serine Endopeptidases/genetics ; beta-Lactamases/chemistry ; beta-Lactamases/genetics
Chemical Substances	Bacterial Proteins ; Maltose-Binding Proteins ; Membrane Proteins ; Oligopeptides ; Protein Sorting Signals ; TasA protein, Bacillus subtilis ; tyrosyl-arginyl-phenylalanyl-beta--alanine ; Serine Endopeptidases (EC 3.4.21.-) ; type I signal peptidase (EC 3.4.21.89) ; beta-Lactamases (EC 3.5.2.6)
Language	English
Publishing date	2021-09-04
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	60-7
ISSN	1879-2642 ; 1879-2596 ; 1879-260X ; 1872-8006 ; 1879-2618 ; 1879-2650 ; 0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 0925-4439 ; 1874-9399
ISSN (online)	1879-2642 ; 1879-2596 ; 1879-260X ; 1872-8006 ; 1879-2618 ; 1879-2650
ISSN	0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 0925-4439 ; 1874-9399
DOI	10.1016/j.bbamem.2021.183768
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Article ; Online: Gene expression analysis of the Xenopus laevis early limb bud proximodistal axis.

Hudson, Daniel T / Bromell, Jessica S / Day, Robert C / McInnes, Tyler / Ward, Joanna M / Beck, Caroline W

Developmental dynamics : an official publication of the American Association of Anatomists

2022 Volume 251, Issue 11, Page(s) 1880–1896

Abstract: Background: Limb buds develop as bilateral outgrowths of the lateral plate mesoderm and are patterned along three axes. Current models of proximal to distal patterning of early amniote limb buds suggest that two signals, a distal organizing signal from ... ...

Abstract	Background: Limb buds develop as bilateral outgrowths of the lateral plate mesoderm and are patterned along three axes. Current models of proximal to distal patterning of early amniote limb buds suggest that two signals, a distal organizing signal from the apical epithelial ridge (AER, Fgfs) and an opposing proximal (retinoic acid [RA]) act early on pattern this axis. Results: Transcriptional analysis of stage 51 Xenopus laevis hindlimb buds sectioned along the proximal-distal axis showed that the distal region is distinct from the rest of the limb. Expression of capn8.3, a novel calpain, was located in cells immediately flanking the AER. The Wnt antagonist Dkk1 was AER-specific in Xenopus limbs. Two transcription factors, sall1 and zic5, were expressed in distal mesenchyme. Zic5 has no described association with limb development. We also describe expression of two proximal genes, gata5 and tnn, not previously associated with limb development. Differentially expressed genes were associated with Fgf, Wnt, and RA signaling as well as differential cell adhesion and proliferation. Conclusions: We identify new candidate genes for early proximodistal limb patterning. Our analysis of RA-regulated genes supports a role for transient RA gradients in early limb bud in proximal-to-distal patterning in this anamniote model organism.
MeSH term(s)	Animals ; Limb Buds/metabolism ; Xenopus laevis/genetics ; Xenopus laevis/metabolism ; Gene Expression Regulation, Developmental ; Mesoderm/metabolism ; Fibroblast Growth Factors/genetics ; Fibroblast Growth Factors/metabolism ; Tretinoin/metabolism ; Extremities ; Gene Expression ; Ectoderm/metabolism ; DNA-Binding Proteins/genetics ; Nerve Tissue Proteins/genetics ; Xenopus Proteins/genetics ; Xenopus Proteins/metabolism
Chemical Substances	Fibroblast Growth Factors (62031-54-3) ; Tretinoin (5688UTC01R) ; Zic5 protein, Xenopus ; DNA-Binding Proteins ; Nerve Tissue Proteins ; Xenopus Proteins
Language	English
Publishing date	2022-07-20
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1102541-4
ISSN	1097-0177 ; 1058-8388
ISSN (online)	1097-0177
ISSN	1058-8388
DOI	10.1002/dvdy.517
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Article ; Online: Regional differences in cerebrovascular reactivity in response to acute isocapnic hypoxia in healthy humans: Methodological considerations.

Keough, Joanna R G / Cates, Valerie C / Tymko, Michael M / Boulet, Lindsey M / Jamieson, Alenna N / Foster, Glen E / Day, Trevor A

Respiratory physiology & neurobiology

2021 Volume 294, Page(s) 103770

Abstract: The cerebrovasculature responds to blood gas challenges. Regional differences (anterior vs. posterior) in cerebrovascular responses to increases in ... ...

Abstract	The cerebrovasculature responds to blood gas challenges. Regional differences (anterior vs. posterior) in cerebrovascular responses to increases in CO
MeSH term(s)	Adult ; Cerebrovascular Circulation/physiology ; Humans ; Hypoxia/diagnostic imaging ; Hypoxia/physiopathology ; Middle Cerebral Artery/diagnostic imaging ; Middle Cerebral Artery/physiopathology ; Posterior Cerebral Artery/diagnostic imaging ; Posterior Cerebral Artery/physiopathology ; Ultrasonography, Doppler, Transcranial ; Young Adult
Language	English
Publishing date	2021-07-31
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2077867-3
ISSN	1878-1519 ; 1569-9048
ISSN (online)	1878-1519
ISSN	1569-9048
DOI	10.1016/j.resp.2021.103770
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Article: Modular Orthopaedic Tissue Engineering With Implantable Microcarriers and Canine Adipose-Derived Mesenchymal Stromal Cells.

Simitzi, Chara / Vlahovic, Maja / Georgiou, Alex / Keskin-Erdogan, Zalike / Miller, Joanna / Day, Richard M

Frontiers in bioengineering and biotechnology

2020 Volume 8, Page(s) 816

Abstract: Mesenchymal stromal cells (MSC) hold significant potential for tissue engineering applications. Modular tissue engineering involves the use of cellularized "building blocks" that can be assembled via a bottom-up approach into larger tissue-like ... ...

Abstract	Mesenchymal stromal cells (MSC) hold significant potential for tissue engineering applications. Modular tissue engineering involves the use of cellularized "building blocks" that can be assembled via a bottom-up approach into larger tissue-like constructs. This approach emulates more closely the complexity associated hierarchical tissues compared with conventional top-down tissue engineering strategies. The current study describes the combination of biodegradable porous poly(DL-lactide-co-glycolide) (PLGA) TIPS microcarriers with canine adipose-derived MSC (cAdMSC) for use as implantable conformable building blocks in modular tissue engineering applications. Optimal conditions were identified for the attachment and proliferation of cAdMSC on the surface of the microcarriers. Culture of the cellularized microcarriers for 21 days in transwell insert plates under conditions used to induce either chondrogenic or osteogenic differentiation resulted in self-assembly of solid 3D tissue constructs. The tissue constructs exhibited phenotypic characteristics indicative of successful osteogenic or chondrogenic differentiation, as well as viscoelastic mechanical properties. This strategy paves the way to create
Language	English
Publishing date	2020-07-22
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2719493-0
ISSN	2296-4185
ISSN	2296-4185
DOI	10.3389/fbioe.2020.00816
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Article ; Online: New Perspectives on Escherichia coli Signal Peptidase I Substrate Specificity: Investigating Why the TasA Cleavage Site Is Incompatible with LepB Cleavage.

Musik, Joanna E / Poole, Jessica / Day, Christopher J / Haselhorst, Thomas / Jen, Freda E-C / Ve, Thomas / Masic, Veronika / Jennings, Michael P / Zalucki, Yaramah M

Microbiology spectrum

2023 Volume 11, Issue 3, Page(s) e0500522

Abstract: Escherichia coli signal peptidase I (LepB) has been shown to inefficiently cleave secreted proteins with aromatic amino acids at the second position after the signal peptidase cleavage site (P2'). The Bacillus subtilis exported protein TasA contains a ... ...

Abstract	Escherichia coli signal peptidase I (LepB) has been shown to inefficiently cleave secreted proteins with aromatic amino acids at the second position after the signal peptidase cleavage site (P2'). The Bacillus subtilis exported protein TasA contains a phenylalanine at P2', which in B. subtilis is cleaved by a dedicated archaeal-organism-like signal peptidase, SipW. We have previously shown that when the TasA signal peptide is fused to maltose binding protein (MBP) up to the P2' position, the TasA-MBP fusion protein is cleaved very inefficiently by LepB. However, the precise reason why the TasA signal peptide hinders cleavage by LepB is not known. In this study, a set of 11 peptides were designed to mimic the inefficiently cleaved secreted proteins, wild-type TasA and TasA-MBP fusions, to determine whether the peptides interact with and inhibit the function of LepB. The binding affinity and inhibitory potential of the peptides against LepB were assessed by surface plasmon resonance (SPR) and a LepB enzyme activity assay. Molecular modeling of the interaction between TasA signal peptide and LepB indicated that the tryptophan residue at P2 (two amino acids before the cleavage site) inhibited the active site serine-90 residue on LepB from accessing the cleavage site. Replacing the P2 tryptophan with alanine (W26A) allowed for more efficient processing of the signal peptide when the TasA-MBP fusion was expressed in E. coli. The importance of this residue to inhibit signal peptide cleavage and the potential to design LepB inhibitors based on the TasA signal peptide are discussed.
MeSH term(s)	Humans ; Escherichia coli/metabolism ; Escherichia coli Proteins/genetics ; Escherichia coli Proteins/metabolism ; Substrate Specificity ; Tryptophan/metabolism ; Amino Acid Sequence ; Protein Sorting Signals
Chemical Substances	type I signal peptidase (EC 3.4.21.89) ; Escherichia coli Proteins ; Tryptophan (8DUH1N11BX) ; Protein Sorting Signals
Language	English
Publishing date	2023-04-26
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2807133-5
ISSN	2165-0497 ; 2165-0497
ISSN (online)	2165-0497
ISSN	2165-0497
DOI	10.1128/spectrum.05005-22
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Article ; Online: Cardiorespiratory plasticity in humans following two patterns of acute intermittent hypoxia.

Keough, Joanna R G / Tymko, Michael M / Boulet, Lindsey M / Jamieson, Alenna N / Day, Trevor A / Foster, Glen E

Experimental physiology

2021 Volume 106, Issue 7, Page(s) 1524–1534

Abstract: New findings: What is the central question of this study? Do cardiorespiratory experience-dependent effects (EDEs) differ between two different stimulus durations of acute isocapnic intermittent hypoxia (IHx; 5-min vs. 90-s cycles between hypoxia and ... ...

Abstract	New findings: What is the central question of this study? Do cardiorespiratory experience-dependent effects (EDEs) differ between two different stimulus durations of acute isocapnic intermittent hypoxia (IHx; 5-min vs. 90-s cycles between hypoxia and normoxia)? What is the main finding and its importance? There was long-term facilitation in ventilation and blood pressure in both IHx protocols, but there was no evidence of progressive augmentation or post-hypoxia frequency decline. Not all EDEs described in animal models translate to acute isocapnic IHx responses in humans, and cardiorespiratory responses to 5-min versus 90-s on/off IHx protocols are largely similar. Abstract: Peripheral respiratory chemoreceptors monitor breath-by-breath changes in arterial CO
MeSH term(s)	Animals ; Chemoreceptor Cells ; Female ; Humans ; Hypoxia ; Lung ; Respiration ; Tidal Volume
Language	English
Publishing date	2021-06-08
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1016295-1
ISSN	1469-445X ; 0958-0670
ISSN (online)	1469-445X
ISSN	0958-0670
DOI	10.1113/EP089443
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Article ; Online: Efficient function of signal peptidase 1 of Escherichia coli is partly determined by residues in the mature N-terminus of exported proteins.

Musik, Joanna E / Zalucki, Yaramah M / Day, Christopher J / Jennings, Michael P

Biochimica et biophysica acta. Biomembranes

2019 Volume 1861, Issue 5, Page(s) 1018–1022

Abstract: Exported proteins require an N-terminal signal peptide to direct them from the cytoplasm to the periplasm. Once the protein has been translocated across the cytoplasmic membrane, the signal peptide is cleaved by a signal peptidase, allowing the remainder ...

Abstract	Exported proteins require an N-terminal signal peptide to direct them from the cytoplasm to the periplasm. Once the protein has been translocated across the cytoplasmic membrane, the signal peptide is cleaved by a signal peptidase, allowing the remainder of the protein to fold into its mature state in the periplasm. Signal peptidase I (LepB) cleaves non-lipoproteins and recognises the sequence Ala-X-Ala. Amino acids present at the N-terminus of mature, exported proteins have been shown to affect the efficiency at which the protein is exported. Here we investigated a bias against aromatic amino acids at the second position in the mature protein (P2'). Maltose binding protein (MBP) was mutated to introduce aromatic amino acids (tryptophan, tyrosine and phenylalanine) at P2'. All mutants with aromatic amino acids at P2' were exported less efficiently as indicated by a slight increase in precursor protein in vivo. Binding of LepB to peptides that encompass the MBP cleavage site were analysed using surface plasmon resonance. These studies showed peptides with an aromatic amino acid at P2' had a slower off rate, due to a significantly higher binding affinity for LepB. These data are consistent with the accumulation of small amounts of preMBP in purified protein samples. Hence, the reason for the lack of aromatic amino acids at P2' in E. coli is likely due to interference with efficient LepB activity. These data and previous bioinformatics strongly suggest that aromatic amino acids are not preferred at P2' and this should be incorporated into signal peptide prediction algorithms.
MeSH term(s)	Amino Acids, Aromatic/analysis ; Amino Acids, Aromatic/genetics ; Amino Acids, Aromatic/metabolism ; Cloning, Molecular ; Escherichia coli/enzymology ; Escherichia coli/metabolism ; Escherichia coli Proteins/analysis ; Escherichia coli Proteins/chemistry ; Escherichia coli Proteins/genetics ; Escherichia coli Proteins/metabolism ; Membrane Proteins/analysis ; Membrane Proteins/genetics ; Membrane Proteins/metabolism ; Protein Transport ; Serine Endopeptidases/analysis ; Serine Endopeptidases/genetics ; Serine Endopeptidases/metabolism
Chemical Substances	Amino Acids, Aromatic ; Escherichia coli Proteins ; Membrane Proteins ; Serine Endopeptidases (EC 3.4.21.-) ; type I signal peptidase (EC 3.4.21.89)
Language	English
Publishing date	2019-03-05
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	60-7
ISSN	1879-2642 ; 1879-2596 ; 1879-260X ; 1872-8006 ; 1879-2618 ; 1879-2650 ; 0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 0925-4439 ; 1874-9399
ISSN (online)	1879-2642 ; 1879-2596 ; 1879-260X ; 1872-8006 ; 1879-2618 ; 1879-2650
ISSN	0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 0925-4439 ; 1874-9399
DOI	10.1016/j.bbamem.2019.03.001
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