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  1. Article ; Online: CRISPR editing in the lung with novel lipids.

    Elia, Uri / Kon, Edo / Peer, Dan

    Nature biotechnology

    2023  Volume 41, Issue 10, Page(s) 1387–1388

    Language English
    Publishing date 2023-03-30
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1311932-1
    ISSN 1546-1696 ; 1087-0156
    ISSN (online) 1546-1696
    ISSN 1087-0156
    DOI 10.1038/s41587-023-01744-5
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    Zs.A 2167: Show issues Location:
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  2. Article ; Online: Principles for designing an optimal mRNA lipid nanoparticle vaccine.

    Kon, Edo / Elia, Uri / Peer, Dan

    Current opinion in biotechnology

    2021  Volume 73, Page(s) 329–336

    Abstract: mRNA Lipid nanoparticles (LNPs) have recently been propelled onto the center stage of therapeutic platforms due to the success of the SARS-CoV-2 mRNA LNP vaccines (mRNA-1273 and BNT162b2), with billions of mRNA vaccine doses already shipped worldwide. ... ...

    Abstract mRNA Lipid nanoparticles (LNPs) have recently been propelled onto the center stage of therapeutic platforms due to the success of the SARS-CoV-2 mRNA LNP vaccines (mRNA-1273 and BNT162b2), with billions of mRNA vaccine doses already shipped worldwide. While mRNA vaccines seem like an overnight success to some, they are in fact a result of decades of scientific research. The advantage of mRNA-LNP vaccines lies in the modularity of the platform and the rapid manufacturing capabilities. However, there is a multitude of choices to be made when designing an optimal mRNA-LNP vaccine regarding efficacy, stability and toxicity. Herein, we provide a brief on what we consider to be the most important aspects to cover when designing mRNA-LNPs from what is currently known and how to optimize them. Lastly, we give our perspective on which of these aspects is most crucial and what we believe are the next steps required to advance the field.
    MeSH term(s) BNT162 Vaccine ; COVID-19 ; Humans ; Liposomes ; Nanoparticles ; Vaccine Development ; Vaccines, Synthetic ; mRNA Vaccines
    Chemical Substances Lipid Nanoparticles ; Liposomes ; Vaccines, Synthetic ; mRNA Vaccines ; BNT162 Vaccine (N38TVC63NU)
    Language English
    Publishing date 2021-10-26
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 1052045-4
    ISSN 1879-0429 ; 0958-1669
    ISSN (online) 1879-0429
    ISSN 0958-1669
    DOI 10.1016/j.copbio.2021.09.016
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  3. Article: Principles for designing an optimal mRNA lipid nanoparticle vaccine

    Kon, Edo / Elia, Uri / Peer, Dan

    Current opinion in biotechnology. 2022 Feb., v. 73

    2022  

    Abstract: mRNA Lipid nanoparticles (LNPs) have recently been propelled onto the center stage of therapeutic platforms due to the success of the SARS-CoV-2 mRNA LNP vaccines (mRNA-1273 and BNT162b2), with billions of mRNA vaccine doses already shipped worldwide. ... ...

    Abstract mRNA Lipid nanoparticles (LNPs) have recently been propelled onto the center stage of therapeutic platforms due to the success of the SARS-CoV-2 mRNA LNP vaccines (mRNA-1273 and BNT162b2), with billions of mRNA vaccine doses already shipped worldwide. While mRNA vaccines seem like an overnight success to some, they are in fact a result of decades of scientific research. The advantage of mRNA-LNP vaccines lies in the modularity of the platform and the rapid manufacturing capabilities. However, there is a multitude of choices to be made when designing an optimal mRNA-LNP vaccine regarding efficacy, stability and toxicity. Herein, we provide a brief on what we consider to be the most important aspects to cover when designing mRNA-LNPs from what is currently known and how to optimize them. Lastly, we give our perspective on which of these aspects is most crucial and what we believe are the next steps required to advance the field.
    Keywords Severe acute respiratory syndrome coronavirus 2 ; biotechnology ; lipids ; therapeutics ; toxicity ; vaccines
    Language English
    Dates of publication 2022-02
    Size p. 329-336.
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 1052045-4
    ISSN 1879-0429 ; 0958-1669
    ISSN (online) 1879-0429
    ISSN 0958-1669
    DOI 10.1016/j.copbio.2021.09.016
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  4. Article: A Novel Approach to Vaccine Development: Concomitant Pathogen Inactivation and Host Immune Stimulation by Peroxynitrite.

    Rotem, Shahar / Bar-Haim, Erez / Elia, Uri / Cohen, Hila / Lazar, Shirley / Cohen, Ofer / Chitlaru, Theodor / Gal, Yoav

    Vaccines

    2022  Volume 10, Issue 10

    Abstract: The design of efficient vaccines for long-term protective immunity against pathogens represents an objective of utmost public health priority. In general, live attenuated vaccines are considered to be more effective than inactivated pathogens, yet ... ...

    Abstract The design of efficient vaccines for long-term protective immunity against pathogens represents an objective of utmost public health priority. In general, live attenuated vaccines are considered to be more effective than inactivated pathogens, yet potentially more reactogenic. Accordingly, inactivation protocols which do not compromise the pathogen's ability to elicit protective immunity are highly beneficial. One of the sentinel mechanisms of the host innate immune system relies on the production of reactive nitrogen intermediates (RNI), which efficiently inactivate pathogens. Peroxynitrite (PN) is a prevalent RNI, assembled spontaneously upon the interaction of nitric oxide (NO) with superoxide. PN exerts its bactericidal effect by via the efficient oxidation of a broad range of biological molecules. Furthermore, the interaction of PN with proteins results in structural/chemical modifications, such as the oxidation of tryptophan, tyrosine, and cysteine residues, as well as the formation of carbonyl, dityrosine, and nitrotyrosine (NT). In addition to their role in innate immunity, these PN-mediated modifications of pathogen components may also augment the antigenicity of pathogen peptides and proteins, hence contributing to specific humoral responses. In the study reported here, a novel approach for vaccine development, consisting of pathogen inactivation by PN, combined with increased immunity of NT-containing peptides, is implemented as a proof-of-concept for vaccination against the intracellular pathogen
    Language English
    Publishing date 2022-09-22
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2703319-3
    ISSN 2076-393X
    ISSN 2076-393X
    DOI 10.3390/vaccines10101593
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  5. Article ; Online: T Cell Response following Anti-COVID-19 BNT162b2 Vaccination Is Maintained against the SARS-CoV-2 Omicron B.1.1.529 Variant of Concern.

    Cohen, Hila / Rotem, Shahar / Elia, Uri / Bilinsky, Gal / Levy, Itzchak / Chitlaru, Theodor / Bar-Haim, Erez

    Viruses

    2022  Volume 14, Issue 2

    Abstract: The progression of the COVID-19 pandemic has led to the emergence of variants of concern (VOC), which may compromise the efficacy of the currently administered vaccines. Antigenic drift can potentially bring about reduced protective T cell immunity and, ... ...

    Abstract The progression of the COVID-19 pandemic has led to the emergence of variants of concern (VOC), which may compromise the efficacy of the currently administered vaccines. Antigenic drift can potentially bring about reduced protective T cell immunity and, consequently, more severe disease manifestations. To assess this possibility, the T cell responses to the wild-type Wuhan-1 SARS-CoV-2 ancestral spike protein and the Omicron B.1.1.529 spike protein were compared. Accordingly, peripheral blood mononuclear cells (PBMC) were collected from eight healthy volunteers 4-5 months following a third vaccination with BNT162b2, and stimulated with overlapping peptide libraries representing the spike of either the ancestral or the Omicron SARS-CoV-2 virus variants. Quantification of the specific T cells was carried out by a fluorescent ELISPOT assay, monitoring cells secreting interferon-gamma (IFNg), interleukin-10 (IL-10) and interleukin-4 (IL-4). For all the examined individuals, comparable levels of reactivity to both forms of spike protein were determined. In addition, a dominant Th1 response was observed, manifested mainly by IFNg-secreting cells and only limited numbers of IL-10- and IL-4-secreting cells. The data demonstrate stable T cell activity in response to the emerging Omicron variant in the tested individuals; therefore, the protective immunity to the variant following BNT162b2 vaccination is not significantly affected.
    MeSH term(s) Adult ; Antibodies, Neutralizing/blood ; Antibodies, Neutralizing/immunology ; Antibodies, Viral/blood ; Antibodies, Viral/immunology ; BNT162 Vaccine/administration & dosage ; BNT162 Vaccine/immunology ; COVID-19/immunology ; COVID-19/prevention & control ; Cytokines/analysis ; Cytokines/immunology ; Enzyme-Linked Immunospot Assay ; Female ; Humans ; Interferon-gamma/analysis ; Interferon-gamma/immunology ; Male ; Middle Aged ; SARS-CoV-2/immunology ; Spike Glycoprotein, Coronavirus/immunology ; T-Lymphocytes/immunology ; Th1 Cells/immunology ; Young Adult
    Chemical Substances Antibodies, Neutralizing ; Antibodies, Viral ; Cytokines ; Spike Glycoprotein, Coronavirus ; spike protein, SARS-CoV-2 ; Interferon-gamma (82115-62-6) ; BNT162 Vaccine (N38TVC63NU)
    Language English
    Publishing date 2022-02-08
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2516098-9
    ISSN 1999-4915 ; 1999-4915
    ISSN (online) 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v14020347
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  6. Article ; Online: Recapitulating Actin Module Organization in the

    Krishnan, Ramesh Kumar / Baskar, Raju / Anna, Bakhrat / Elia, Natalie / Boermel, Mandy / Bausch, Andreas R / Abdu, Uri

    International journal of molecular sciences

    2021  Volume 22, Issue 8

    Abstract: The generation of F-actin bundles is controlled by the action of actin-binding proteins. ... ...

    Abstract The generation of F-actin bundles is controlled by the action of actin-binding proteins. In
    MeSH term(s) Actin Cytoskeleton/metabolism ; Actins/metabolism ; Animals ; Cytoskeletal Proteins/metabolism ; Drosophila Proteins/metabolism ; Drosophila melanogaster/cytology ; Drosophila melanogaster/metabolism ; Germ Cells/metabolism ; Oocytes/cytology ; Oocytes/metabolism ; Structure-Activity Relationship
    Chemical Substances Actins ; Cytoskeletal Proteins ; Drosophila Proteins ; actin-modulating proteins
    Language English
    Publishing date 2021-04-13
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms22084006
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  7. Article: T Cell Response following Anti-COVID-19 BNT162b2 Vaccination Is Maintained against the SARS-CoV-2 Omicron B.1.1.529 Variant of Concern

    Cohen, Hila / Rotem, Shahar / Elia, Uri / Bilinsky, Gal / Levy, Itzchak / Chitlaru, Theodor / Bar-Haim, Erez

    Viruses. 2022 Feb. 08, v. 14, no. 2

    2022  

    Abstract: The progression of the COVID-19 pandemic has led to the emergence of variants of concern (VOC), which may compromise the efficacy of the currently administered vaccines. Antigenic drift can potentially bring about reduced protective T cell immunity and, ... ...

    Abstract The progression of the COVID-19 pandemic has led to the emergence of variants of concern (VOC), which may compromise the efficacy of the currently administered vaccines. Antigenic drift can potentially bring about reduced protective T cell immunity and, consequently, more severe disease manifestations. To assess this possibility, the T cell responses to the wild-type Wuhan-1 SARS-CoV-2 ancestral spike protein and the Omicron B.1.1.529 spike protein were compared. Accordingly, peripheral blood mononuclear cells (PBMC) were collected from eight healthy volunteers 4–5 months following a third vaccination with BNT162b2, and stimulated with overlapping peptide libraries representing the spike of either the ancestral or the Omicron SARS-CoV-2 virus variants. Quantification of the specific T cells was carried out by a fluorescent ELISPOT assay, monitoring cells secreting interferon-gamma (IFNg), interleukin-10 (IL-10) and interleukin-4 (IL-4). For all the examined individuals, comparable levels of reactivity to both forms of spike protein were determined. In addition, a dominant Th1 response was observed, manifested mainly by IFNg-secreting cells and only limited numbers of IL-10- and IL-4-secreting cells. The data demonstrate stable T cell activity in response to the emerging Omicron variant in the tested individuals; therefore, the protective immunity to the variant following BNT162b2 vaccination is not significantly affected.
    Keywords COVID-19 infection ; Severe acute respiratory syndrome coronavirus 2 ; T-lymphocytes ; antigenic variation ; disease severity ; fluorescence ; immunity ; interferon-gamma ; interleukin-10 ; interleukin-4 ; vaccination ; viruses
    Language English
    Dates of publication 2022-0208
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2516098-9
    ISSN 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v14020347
    Database NAL-Catalogue (AGRICOLA)

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  8. Article ; Online: Examining the utility of nonlinear machine learning approaches versus linear regression for predicting body image outcomes: The U.S. Body Project I.

    Liang, Dehua / Frederick, David A / Lledo, Elia E / Rosenfield, Natalia / Berardi, Vincent / Linstead, Erik / Maoz, Uri

    Body image

    2022  Volume 41, Page(s) 32–45

    Abstract: Most body image studies assess only linear relations between predictors and outcome variables, relying on techniques such as multiple Linear Regression. These predictor variables are often validated multi-item measures that aggregate individual items ... ...

    Abstract Most body image studies assess only linear relations between predictors and outcome variables, relying on techniques such as multiple Linear Regression. These predictor variables are often validated multi-item measures that aggregate individual items into a single scale. The advent of machine learning has made it possible to apply Nonlinear Regression algorithms-such as Random Forest and Deep Neural Networks-to identify potentially complex linear and nonlinear connections between a multitude of predictors (e.g., all individual items from a scale) and outcome (output) variables. Using a national dataset, we tested the extent to which these techniques allowed us to explain a greater share of the variance in body-image outcomes (adjusted R
    MeSH term(s) Body Image/psychology ; Humans ; Linear Models ; Machine Learning
    Language English
    Publishing date 2022-02-25
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2211449-X
    ISSN 1873-6807 ; 1740-1445
    ISSN (online) 1873-6807
    ISSN 1740-1445
    DOI 10.1016/j.bodyim.2022.01.013
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  9. Article ; Online: A single-dose F1-based mRNA-LNP vaccine provides protection against the lethal plague bacterium.

    Kon, Edo / Levy, Yinon / Elia, Uri / Cohen, Hila / Hazan-Halevy, Inbal / Aftalion, Moshe / Ezra, Assaf / Bar-Haim, Erez / Naidu, Gonna Somu / Diesendruck, Yael / Rotem, Shahar / Ad-El, Nitay / Goldsmith, Meir / Mamroud, Emanuelle / Peer, Dan / Cohen, Ofer

    Science advances

    2023  Volume 9, Issue 10, Page(s) eadg1036

    Abstract: Messenger RNA (mRNA) lipid nanoparticle (LNP) vaccines have emerged as an effective vaccination strategy. Although currently applied toward viral pathogens, data concerning the platform's effectiveness against bacterial pathogens are limited. Here, we ... ...

    Abstract Messenger RNA (mRNA) lipid nanoparticle (LNP) vaccines have emerged as an effective vaccination strategy. Although currently applied toward viral pathogens, data concerning the platform's effectiveness against bacterial pathogens are limited. Here, we developed an effective mRNA-LNP vaccine against a lethal bacterial pathogen by optimizing mRNA payload guanine and cytosine content and antigen design. We designed a nucleoside-modified mRNA-LNP vaccine based on the bacterial F1 capsule antigen, a major protective component of
    MeSH term(s) Mice ; Animals ; Plague/prevention & control ; Plague Vaccine/genetics ; Bacterial Proteins/genetics ; Mice, Inbred C57BL ; Yersinia pestis/genetics ; Antigens, Bacterial/genetics
    Chemical Substances Plague Vaccine ; Bacterial Proteins ; Antigens, Bacterial
    Language English
    Publishing date 2023-03-08
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2810933-8
    ISSN 2375-2548 ; 2375-2548
    ISSN (online) 2375-2548
    ISSN 2375-2548
    DOI 10.1126/sciadv.adg1036
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  10. Article: Humoral and T-Cell Response before and after a Fourth BNT162b2 Vaccine Dose in Adults ≥60 Years.

    Bar-Haim, Erez / Eliakim-Raz, Noa / Stemmer, Amos / Cohen, Hila / Elia, Uri / Ness, Asaf / Awwad, Muhammad / Ghantous, Nassem / Moskovits, Neta / Rotem, Shahar / Stemmer, Salomon M

    Journal of clinical medicine

    2022  Volume 11, Issue 9

    Abstract: Both humoral and cellular anamnestic responses are significant for protective immunity against SARS-CoV-2. In the current study, the responses in elderly people before and after a fourth vaccine dose of BNT162b2 were compared to those of individuals ... ...

    Abstract Both humoral and cellular anamnestic responses are significant for protective immunity against SARS-CoV-2. In the current study, the responses in elderly people before and after a fourth vaccine dose of BNT162b2 were compared to those of individuals immunized with three vaccine doses. Although a boost effect was observed, the high response following the third administration questions the necessity of an early fourth boost.
    Language English
    Publishing date 2022-05-08
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662592-1
    ISSN 2077-0383
    ISSN 2077-0383
    DOI 10.3390/jcm11092649
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