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Article ; Online: Right atrial cavernous hemangioma.

Shashikanth, Manikappa / Nicola, Sandler / Yi, Chen / Julian, Smith

2020 Volume 23, Issue 3, Page(s) 335–337

Abstract: Benign cardiac hemangiomas are rare tumors that may present in or out side of the heart, epicardium being the most common site. Echocardiography is the method of choice in diagnosing cardiac masses and though 3D TEE may seem to add exact information ... ...

Abstract	Benign cardiac hemangiomas are rare tumors that may present in or out side of the heart, epicardium being the most common site. Echocardiography is the method of choice in diagnosing cardiac masses and though 3D TEE may seem to add exact information about the location, the time constraint in doing a comprehensive examination along with 3D rendering inside operation room may become an hindrance.
MeSH term(s)	Echocardiography/methods ; Female ; Heart Atria/diagnostic imaging ; Heart Atria/surgery ; Heart Neoplasms/diagnostic imaging ; Heart Neoplasms/surgery ; Hemangioma, Cavernous/diagnostic imaging ; Hemangioma, Cavernous/surgery ; Humans ; Middle Aged ; Tomography, X-Ray Computed/methods
Language	English
Publishing date	2020-07-20
Publishing country	India
Document type	Case Reports ; Journal Article
ZDB-ID	2106866-5
ISSN	0974-5181 ; 0971-9784
ISSN (online)	0974-5181
ISSN	0971-9784
DOI	10.4103/aca.ACA_58_19
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Article: Impact of COVID-19 on Respiratory Admissions in a Tertiary Pediatric Intensive Care Unit.

Ambati, Shashikanth / Mihic, Maya / Wilkinson, Kristy / Sanchez, Javier L / Pezzano, Chad

Cureus

2022 Volume 14, Issue 5, Page(s) e25369

Abstract: Background Pediatric inpatient admissions for viral respiratory infections decreased worldwide during the early part of the coronavirus disease 2019 (COVID-19) pandemic. This was likely due to social distancing measures and mask mandates leading to a ... ...

Abstract	Background Pediatric inpatient admissions for viral respiratory infections decreased worldwide during the early part of the coronavirus disease 2019 (COVID-19) pandemic. This was likely due to social distancing measures and mask mandates leading to a decreased spread of viruses. We question if there was an increase in respiratory admissions during the winter of 2020-2021 due to the overlap of seasonal respiratory viruses and COVID-19 and the severity of those admissions. Methods We performed a single-center retrospective chart review of all respiratory admissions to our pediatric intensive care unit (PICU) from October to April during the years 2018-2019, 2019-2020, and 2020-2021. We compared the total number of respiratory admissions from different viruses and respiratory admissions by diagnoses among those time periods. Second, we compared the PICU length of stay and duration of mechanical ventilation (both invasive and non-invasive) for these respiratory admissions during those years. Results We saw a drastic decrease in the total respiratory admissions to the PICU in 2020-2021 compared to the same period of time in the last two years. The greatest contributor to this decrease was admissions secondary to bronchiolitis. We noticed a statistically significant decrease in both asthma (p<0.001) and chronic respiratory failure admissions (p=0.0029) during the pandemic winter compared to previous winters. Although, the total number of all respiratory viral admissions is not significant, admissions specific to the respiratory syncytial virus (RSV) (p<0.0001), rhino-enterovirus (p<0.0001), and multi-virus (p=0.0016), achieved statistical significance. There was no statistical difference between the PICU length of stay and duration of mechanical ventilation during the three years. Conclusion Despite a decrease in pediatric respiratory admissions during the COVID-19 pandemic, the severity of illness based on length of stay in the PICU and length of time on respiratory support remains unchanged compared to the previous two years.
Language	English
Publishing date	2022-05-26
Publishing country	United States
Document type	Journal Article
ZDB-ID	2747273-5
ISSN	2168-8184
ISSN	2168-8184
DOI	10.7759/cureus.25369
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Article ; Online: Differentiation of the 50B11 dorsal ganglion cells into NGF and GDNF responsive nociceptor subtypes.

Dusan, Matusica / Jastrow, Canlas / Alyce, Martin M / Yingkai, Wei / Shashikanth, Marri / Andelain, Erickson / Christine, Barry M / Stuart, Brierley M / Oliver, Best G / Michael, Michael Z / Nicolas, Voelcker H / Damien, Keating J / Rainer, Haberberger V

Molecular pain

2020 Volume 16, Page(s) 1744806920970368

Abstract: ... sensory neuron model expressing markers characteristic of NGF and GDNF-dependent C-fibre nociceptors ...

Abstract	The embryonic rat dorsal root ganglion (DRG) neuron-derived 50B11 cell line is a promising sensory neuron model expressing markers characteristic of NGF and GDNF-dependent C-fibre nociceptors. Whether these cells have the capacity to develop into distinct nociceptive subtypes based on NGF- or GDNF-dependence has not been investigated. Here we show that by augmenting forskolin (FSK) and growth factor supplementation with NGF or GDNF, 50B11 cultures can be driven to acquire differential functional responses to common nociceptive agonists capsaicin and ATP respectively. In addition, to previous studies, we also demonstrate that a differentiated neuronal phenotype can be maintained for up to 7 days. Western blot analysis of nociceptive marker proteins further demonstrates that the 50B11 cells partially recapitulate the functional phenotypes of classical NGF-dependent (peptidergic) and GDNF-dependent (non-peptidergic) neuronal subtypes described in DRGs. Further, 50B11 cells differentiated with NGF/FSK, but not GDNF/FSK, show sensitization to acute prostaglandin E2 treatment. Finally, RNA-Seq analysis confirms that differentiation with NGF/FSK or GDNF/FSK produces two 50B11 cell subtypes with distinct transcriptome expression profiles. Gene ontology comparison of the two subtypes of differentiated 50B11 cells to rodent DRG neurons studies shows significant overlap in matching or partially matching categories. This transcriptomic analysis will aid future suitability assessment of the 50B11 cells as a high-throughput nociceptor model for a broad range of experimental applications. In conclusion, this study shows that the 50B11 cell line is capable of partially recapitulating features of two distinct types of embryonic NGF and GDNF-dependent nociceptor-like cells.
MeSH term(s)	Action Potentials/drug effects ; Adenosine Triphosphate/pharmacology ; Animals ; Biomarkers/metabolism ; Capsaicin/pharmacology ; Cell Differentiation/drug effects ; Cell Differentiation/genetics ; Cell Line ; Cell Shape/drug effects ; Colforsin/pharmacology ; Dinoprostone/pharmacology ; Ganglia, Spinal/cytology ; Gene Expression Regulation/drug effects ; Genetic Variation ; Glial Cell Line-Derived Neurotrophic Factor/pharmacology ; Nerve Growth Factor/pharmacology ; Neuronal Outgrowth/drug effects ; Neurons/drug effects ; Neurons/metabolism ; Nociceptors/cytology ; Nociceptors/drug effects ; Phenotype ; Principal Component Analysis ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Rats ; Sodium Channels/metabolism
Chemical Substances	Biomarkers ; Glial Cell Line-Derived Neurotrophic Factor ; RNA, Messenger ; Sodium Channels ; Colforsin (1F7A44V6OU) ; Adenosine Triphosphate (8L70Q75FXE) ; Nerve Growth Factor (9061-61-4) ; Dinoprostone (K7Q1JQR04M) ; Capsaicin (S07O44R1ZM)
Language	English
Publishing date	2020-12-11
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2174252-2
ISSN	1744-8069 ; 1744-8069
ISSN (online)	1744-8069
ISSN	1744-8069
DOI	10.1177/1744806920970368
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Article ; Online: Levamisole and low-dose prednisolone in the treatment of reccurent aphthous stomatitis.

Sharda, Neelkamal / Shashikanth, M C / Kant, Priyanka / Jain, Manika

Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology

2014 Volume 43, Issue 4, Page(s) 309–316

Abstract: Context: Recurrent aphthous stomatitis (RAS) is the most common oral mucosal disease. However, the available therapies for RAS only relieve symptoms and do not provide a cure.: Aims: This study assessed the response to treatment with levamisole and ... ...

Abstract	Context: Recurrent aphthous stomatitis (RAS) is the most common oral mucosal disease. However, the available therapies for RAS only relieve symptoms and do not provide a cure. Aims: This study assessed the response to treatment with levamisole and low-dose prednisolone drug combination in patients with RAS. Methods and material: Fifty RAS subjects were enrolled in the single-blind randomized placebo-controlled trial. Study medications were administered thrice daily for 3 consecutive days/week for 3 consecutive weeks. Patients in Group 1 received placebo, Group 2 received levamisole (50 mg) and Group 3 received levamisole (50 mg) and low-dose prednisolone (5 mg). Patients were followed up for 60 days after treatment. Response to treatment was assessed using the following clinical parameters: pain due to ulcers, number of ulcers/episode, size of ulcers, duration of ulcers, and frequency of ulcers (episodes/month). Statistical analysis used: Mann–Whitney U-test. Results: A statistically significant improvement was noted in all parameters except for the size of ulcers in patients treated with levamisole alone and with combination of levamisole and low-dose prednisolone. There was no statistically significant improvement in the placebo group. Both active groups had significantly better improvement when compared to placebo group, while there was no significant difference between the two active groups. Conclusions: Levamisole alone and combination of levamisole and low-dose prednisolone are effective modes of therapy for RAS.
MeSH term(s)	Adjuvants, Immunologic/administration & dosage ; Adjuvants, Immunologic/therapeutic use ; Adolescent ; Adult ; Anti-Inflammatory Agents/administration & dosage ; Drug Combinations ; Female ; Follow-Up Studies ; Humans ; Levamisole/administration & dosage ; Levamisole/therapeutic use ; Male ; Middle Aged ; Pain Measurement ; Placebos ; Prednisolone/administration & dosage ; Recurrence ; Single-Blind Method ; Stomatitis, Aphthous/drug therapy ; Stomatitis, Aphthous/pathology ; Time Factors ; Young Adult
Chemical Substances	Adjuvants, Immunologic ; Anti-Inflammatory Agents ; Drug Combinations ; Placebos ; Levamisole (2880D3468G) ; Prednisolone (9PHQ9Y1OLM)
Language	English
Publishing date	2014-04-29
Publishing country	Denmark
Document type	Comparative Study ; Journal Article ; Randomized Controlled Trial
ZDB-ID	1021270-x
ISSN	1600-0714 ; 0904-2512
ISSN (online)	1600-0714
ISSN	0904-2512
DOI	10.1111/jop.12126
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Article: Pachyonychia congenita tarda: A rare case report.

Moger, Ganapathi / Shashikanth, M C / Chandrashekar, K T / Kurein, Sophia

Contemporary clinical dentistry

2013 Volume 4, Issue 3, Page(s) 409–411

Abstract: Pachyonychia congenita is a rare, but well-characterized autosomal dominant disorder of keratinization. It usually begins within the first few months of life. Here, we are presenting a rare case, which started at the age of 10 years of life and is known ... ...

Abstract	Pachyonychia congenita is a rare, but well-characterized autosomal dominant disorder of keratinization. It usually begins within the first few months of life. Here, we are presenting a rare case, which started at the age of 10 years of life and is known as pachyonychia congenita tarda. The case is being reported for its rarer occurrence as the patient had oral leukokeratosis and angular cheilosis present in the same type of the syndrome (Jadassohn-Lewandowsky syndrome), which is still uncommon.
Language	English
Publishing date	2013-10-11
Publishing country	India
Document type	Case Reports
ZDB-ID	2582352-8
ISSN	0976-2361 ; 0976-237X
ISSN (online)	0976-2361
ISSN	0976-237X
DOI	10.4103/0976-237X.118374
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Article ; Online: A Trifunctional Theranostic Ligand Targeting Fibroblast Activation Protein-α (FAPα).

Kelly, James M / Jeitner, Thomas M / Ponnala, Shashikanth / Williams, Clarence / Nikolopoulou, Anastasia / DiMagno, Stephen G / Babich, John W

Molecular imaging and biology

2021 Volume 23, Issue 5, Page(s) 686–696

Abstract: Purpose: Fibroblast activation protein-α (FAPα) is uniquely expressed in activated fibroblasts, including cancer-associated fibroblasts that populate tumor stroma and contribute to proliferation and immunosuppression. Radiolabeled FAPα inhibitors enable ...

Abstract	Purpose: Fibroblast activation protein-α (FAPα) is uniquely expressed in activated fibroblasts, including cancer-associated fibroblasts that populate tumor stroma and contribute to proliferation and immunosuppression. Radiolabeled FAPα inhibitors enable imaging of multiple human cancers, but time-dependent clearance from tumors currently limits their utility as FAPα-targeted radiotherapeutics. We sought to increase the area under the curve (AUC) by constructing a trifunctional ligand that binds FAPα with high affinity and also binds albumin and theranostic radiometals. Procedures: RPS-309 comprised a FAPα-targeting moiety, an albumin-binding group, and 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA). Inhibition of recombinant human FAPα (rhFAPα) was determined by colorimetric assay. Affinity for human serum albumin (HSA) was determined by high-performance affinity chromatography. The tissue distribution of [ Results: RPS-309 inhibits rhFAPα with IC Conclusions: RPS-309 is a high-affinity FAPα inhibitor with prolonged plasma residence. Introduction of the albumin-binding group did not compromise FAPα binding. Although initial tumor uptake was high and FAPα-specific, RPS-309 also progressively cleared from tumors. Nevertheless, RPS-309 incorporates multiple sites in which structural diversity can be introduced, and therefore serves as a platform for future structure-activity relationship studies.
MeSH term(s)	Animals ; Antineoplastic Agents/chemistry ; Antineoplastic Agents/pharmacokinetics ; Cancer-Associated Fibroblasts/metabolism ; Cell Line, Tumor ; Endopeptidases/metabolism ; Female ; Humans ; Ligands ; Membrane Proteins/metabolism ; Mice ; Positron Emission Tomography Computed Tomography ; Precision Medicine/methods ; Radiopharmaceuticals/chemistry ; Radiopharmaceuticals/pharmacokinetics ; Tissue Distribution ; Xenograft Model Antitumor Assays
Chemical Substances	Antineoplastic Agents ; Ligands ; Membrane Proteins ; Radiopharmaceuticals ; Endopeptidases (EC 3.4.-) ; fibroblast activation protein alpha (EC 3.4.21.-)
Language	English
Publishing date	2021-03-15
Publishing country	United States
Document type	Journal Article
ZDB-ID	2079160-4
ISSN	1860-2002 ; 1536-1632
ISSN (online)	1860-2002
ISSN	1536-1632
DOI	10.1007/s11307-021-01593-1
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Article ; Online: Safety of primary nasotracheal intubation in the pediatric intensive care unit (PICU).

Ducharme-Crevier, Laurence / Furlong-Dillard, Jamie / Jung, Philipp / Chiusolo, Fabrizio / Malone, Matthew P / Ambati, Shashikanth / Parsons, Simon J / Krawiec, Conrad / Al-Subu, Awni / Polikoff, Lee A / Napolitano, Natalie / Tarquinio, Keiko M / Shenoi, Asha / Talukdar, Andrea / Mallory, Palen P / Giuliano, John S / Breuer, Ryan K / Kierys, Krista / Kelly, Serena P /

Motomura, Makoto / Sanders, Ron C / Freeman, Ashley / Nagai, Yuki / Glater-Welt, Lily B / Wilson, Joseph / Loi, Mervin / Adu-Darko, Michelle / Shults, Justine / Nadkarni, Vinay / Emeriaud, Guillaume / Nishisaki, Akira

Intensive care medicine. Paediatric and neonatal

2024 Volume 2, Issue 1, Page(s) 7

Abstract: Background: Nasal tracheal intubation (TI) represents a minority of all TI in the pediatric intensive care unit (PICU). The risks and benefits of nasal TI are not well quantified. As such, safety and descriptive data regarding this practice are ... ...

Abstract	Background: Nasal tracheal intubation (TI) represents a minority of all TI in the pediatric intensive care unit (PICU). The risks and benefits of nasal TI are not well quantified. As such, safety and descriptive data regarding this practice are warranted. Methods: We evaluated the association between TI route and safety outcomes in a prospectively collected quality improvement database (National Emergency Airway Registry for Children: NEAR4KIDS) from 2013 to 2020. The primary outcome was severe desaturation (SpO Results: A total of 22,741 TIs [nasal 870 (3.8%), oral 21,871 (96.2%)] were reported from 60 PICUs. Infants were represented in higher proportion in the nasal TI than the oral TI (75.9%, vs 46.2%), as well as children with cardiac conditions (46.9% vs. 14.4%), both Conclusion: In this large international prospective cohort study, the risk of severe peri-intubation complications was not significantly higher. Nasal TI is used in a minority of TI in PICUs, with substantial differences in patient, provider, and practice compared to oral TI.A prospective multicenter trial may be warranted to address the potential selection bias and to confirm the safety of nasal TI.
Language	English
Publishing date	2024-02-23
Publishing country	Switzerland
Document type	Journal Article
ISSN	2731-944X
ISSN (online)	2731-944X
DOI	10.1007/s44253-024-00035-4
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Article ; Online: Analgesic efficacy of diazepam and placebo in patients with temporomandibular disorders: a double blind randomized clinical trial.

Pramod, G V / Shambulingappa, P / Shashikanth, M C / Lele, Shailesh

Indian journal of dental research : official publication of Indian Society for Dental Research

2011 Volume 22, Issue 3, Page(s) 404–409

Abstract: Aim: The aim of the study was to evaluate and compare the analgesic efficacy of placebo and diazepam in patients with temporomandibular disorder.: Materials and methods: Thirty-five patients were recruited with a diagnosis of temporomandibular ... ...

Abstract	Aim: The aim of the study was to evaluate and compare the analgesic efficacy of placebo and diazepam in patients with temporomandibular disorder. Materials and methods: Thirty-five patients were recruited with a diagnosis of temporomandibular disorder based on standard clinical diagnostic criteria for temporomandibular disorder. The patients were put in to one of the two groups: placebo or diazepam at random. The average pain intensity was recorded with visual analog scale (VAS) at pretreatment, at weekly interval till the completion of a three-week trial and at post-treatment visit on the eighth week from baseline. The secondary outcome measures were changes in masticatory muscle tenderness, viz. massater muscle, lateral pterygoid muscle, medial pterygoid muscle and temporalis muscle and changes in mouth opening. Statistical analysis: Intra-group comparison for analgesic efficacy and mouth opening was carried out by Wilcoxon's signed ranked test. Inter-group comparison for analgesic efficacy was also carried out using Mann-Whitney's test. Results: A statistically significant (P<0.01) decrease in temporomandibular disorder pain in the placebo group (65%) and statistically highly significant (P<0.001) decrease in the diazepam group (72%) were observed on VAS after three weeks of treatment. The inter-group comparison demonstrated no statistically significant difference between the groups. Conclusion: This study suggests that the placebo can give near similar results as diazepam can. So the role of placebo should also be considered as one of the important management strategies. In the short term, reduction in the masticatory muscle tenderness and significant improvement in the mouth opening in both the groups were observed.
MeSH term(s)	Adolescent ; Adult ; Diazepam/therapeutic use ; Double-Blind Method ; Facial Pain/complications ; Facial Pain/drug therapy ; Female ; Humans ; Male ; Middle Aged ; Muscle Relaxants, Central/therapeutic use ; Placebo Effect ; Statistics, Nonparametric ; Temporomandibular Joint Disorders/complications ; Temporomandibular Joint Disorders/drug therapy ; Temporomandibular Joint Disorders/psychology ; Young Adult
Chemical Substances	Muscle Relaxants, Central ; Diazepam (Q3JTX2Q7TU)
Language	English
Publishing date	2011-05
Publishing country	India
Document type	Journal Article ; Randomized Controlled Trial
ZDB-ID	1354886-4
ISSN	1998-3603 ; 0970-9290
ISSN (online)	1998-3603
ISSN	0970-9290
DOI	10.4103/0970-9290.87062
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Article: 2,6-Dichloro-phen-yl 4-chloro-benzoate.

Abdoh, M M M / Srinivasa Murthy, V / Manjunath, B C / Shashikanth, S / Lokanath, N K

Acta crystallographica. Section E, Structure reports online

2012 Volume 68, Issue Pt 12, Page(s) o3449

Abstract: In the title compound, C13H7Cl3O2, the dihedral angle between the benzene rings is 82.1 (2)°. The dihedral angle between the CO2 group and its carbon-bonded ring is 14.50 (19)° In the crystal, aromatic π-π stacking inter-actions [minimum ring centroid ... ...

Abstract	In the title compound, C13H7Cl3O2, the dihedral angle between the benzene rings is 82.1 (2)°. The dihedral angle between the CO2 group and its carbon-bonded ring is 14.50 (19)° In the crystal, aromatic π-π stacking inter-actions [minimum ring centroid separation = 3.604 (2) Å] occur.
Language	English
Publishing date	2012-11-24
Publishing country	United States
Document type	Journal Article
ZDB-ID	2041947-8
ISSN	1600-5368
ISSN	1600-5368
DOI	10.1107/S1600536812047204
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Article ; Online: 66

Amor-Coarasa, Alejandro / Kelly, James M / Ponnala, Shashikanth / Nikolopoulou, Anastasia / Williams, Clarence / Babich, John W

Molecules (Basel, Switzerland)

2018 Volume 23, Issue 10

Abstract: Emerging interest in extending the plasma half-life of small molecule radioligands warrants a consideration of the appropriate radionuclide for PET imaging at longer time points (>8 h). Among candidate positron-emitting radionuclides, ...

Abstract	Emerging interest in extending the plasma half-life of small molecule radioligands warrants a consideration of the appropriate radionuclide for PET imaging at longer time points (>8 h). Among candidate positron-emitting radionuclides,
MeSH term(s)	Animals ; Drug Design ; Gallium Radioisotopes/chemistry ; Gallium Radioisotopes/isolation & purification ; Humans ; Ligands ; Metals/chemistry ; Mice ; Neoplasms/diagnostic imaging ; Neoplasms/pathology ; Positron Emission Tomography Computed Tomography ; Positron-Emission Tomography ; Radiopharmaceuticals/chemistry ; Radiopharmaceuticals/isolation & purification ; Solutions ; X-Ray Microtomography
Chemical Substances	Gallium Radioisotopes ; Ligands ; Metals ; Radiopharmaceuticals ; Solutions
Language	English
Publishing date	2018-10-09
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	1413402-0
ISSN	1420-3049 ; 1431-5165 ; 1420-3049
ISSN (online)	1420-3049
ISSN	1431-5165 ; 1420-3049
DOI	10.3390/molecules23102575
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