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  1. Article: Deciphering the Pharmacological Mechanisms of Ma Xing Shi Gan Decoction against COVID-19 through Integrating Network Pharmacology and Experimental Exploration.

    Li, Qianqian / Bai, Chen / Yang, Ruocong / Xing, Weiying / Pang, Xiaohan / Wu, Siying / Liu, Shaoyang / Chen, Jianxin / Liu, Tiegang / Gu, Xiaohong

    Frontiers in pharmacology

    2020  Volume 11, Page(s) 581691

    Abstract: ... viral pneumonia. In this study, we revealed the efficacy and pharmacological mechanism of Ma Xing Shi Gan (MXSG ...

    Abstract The outbreak of new infectious pneumonia caused by SARS-CoV-2 has posed a significant threat to public health, but specific medicines and vaccines are still being developed. Traditional Chinese medicine (TCM) has thousands of years of experience in facing the epidemic disease, such as influenza and viral pneumonia. In this study, we revealed the efficacy and pharmacological mechanism of Ma Xing Shi Gan (MXSG) Decoction against COVID-19. First, we used liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) to analyze the chemical components in MXSG and identified a total of 97 components from MXSG. Then, the intervention pathway of MXSG based on these components was analyzed with network pharmacology, and it was found that the pathways related to the virus infection process were enriched in some of MXSG component targets. Simultaneously, through literature research, it was preliminarily determined that MXSG, which is an essential prescription for treating COVID-19, shared the feature of antiviral, improving clinical symptoms, regulating immune inflammation, and inhibiting lung injury. The regulatory mechanisms associated with its treatment of COVID-19 were proposed. That MXSG might directly inhibit the adsorption and replication of SARS-CoV-2 at the viral entry step. Besides, MXSG might play a critical role in inflammation and immune regulatory, that is, to prevent cytokine storm and relieve lung injury through toll-like receptors signaling pathway. Next, in this study, the regulatory effect of MXSG on inflammatory lung injury was validated through transcriptome results. In summary, MXSG is a relatively active and safe treatment for influenza and viral pneumonia, and its therapeutic effect may be attributed to its antiviral and anti-inflammatory effects.
    Language English
    Publishing date 2020-11-26
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587355-6
    ISSN 1663-9812
    ISSN 1663-9812
    DOI 10.3389/fphar.2020.581691
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Improving Wildfire Danger Assessment Using Time Series Features of Weather and Fuel in the Great Xing’an Mountain Region, China

    Wang, Zili / He, Binbin / Chen, Rui / Fan, Chunquan

    Forests. 2023 May 10, v. 14, no. 5

    2023  

    Abstract: Wildfires directly threaten the safety of life and property. Predicting wildfires with a model driven by wildfire danger factors can significantly reduce losses. Weather conditions continuously influence the drying rate of fuel as well as the occurrence ... ...

    Abstract Wildfires directly threaten the safety of life and property. Predicting wildfires with a model driven by wildfire danger factors can significantly reduce losses. Weather conditions continuously influence the drying rate of fuel as well as the occurrence probability and danger degree of wildfires. Previous studies have paid little attention to the continuous effects of weather and fuel on wildfires. This study improved the accuracy and effect of wildfire danger assessment using the time series features of weather and fuel. First, the time series features of weather and fuel factors within the 16 days before the fire were analyzed. Then, four feature groups were selected—feature group without time series values, feature group with time series values, feature group with Tsfresh transformation of time series values, and feature group with gradient and cumulative transformation of time series values—and three models were trained, respectively: random forest, balanced random forest, and extreme gradient boosting. The results showed that the f1-score of all feature groups with time series values (0.93) increased by 0.15, on average, compared with those without time series values (0.78) for the three models. The feature group with gradient and cumulative features had a more stable prediction accuracy and a more accurate wildfire danger map. The results suggest that using the appropriate time series features of weather and fuel can help improve the precision and effect of the wildfire danger assessment model.
    Keywords fuels ; models ; prediction ; probability ; time series analysis ; weather ; wildfires ; China
    Language English
    Dates of publication 2023-0510
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article ; Online
    ZDB-ID 2527081-3
    ISSN 1999-4907
    ISSN 1999-4907
    DOI 10.3390/f14050986
    Database NAL-Catalogue (AGRICOLA)

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  3. Article ; Online: The TCM prescription Ma-xing-shi-gan-tang inhibits Streptococcus pneumoniae pathogenesis by targeting pneumolysin.

    Guo, Tingting / Guo, Yinan / Liu, Qingbing / Xu, Yan / Wei, Lina / Wang, Zhongtian / Chen, Si / Wang, Caiwen / Tian, Ye / Cui, Jie / Wang, Yijie / Wang, Yanbo / Sun, Liping

    Journal of ethnopharmacology

    2021  Volume 275, Page(s) 114133

    Abstract: Ethnopharmacological relevance: Ma-xing-shi-gan-tang (MXSGT), which is documented in the Treatise ...

    Abstract Ethnopharmacological relevance: Ma-xing-shi-gan-tang (MXSGT), which is documented in the Treatise on Febrile Diseases and is a therapeutic drug, is a well-known classic prescription in China and has been widely studied. Previous studies have shown that MXSGT has various pharmacological activities, including anti-influenza virus activity, and ameliorates microvascular hyperpermeability and inflammatory reactions. However, no study has reported the effect of MXSGT in the treatment of bacterial pneumonia.
    Aim of the study: In this study, the potential inhibition of MXSGT against the virulence of S. pneumoniae by targeting PLY was investigated.
    Materials and methods: First, HPLC analysis was used to determine the main components of MXSGT. Then PLY protein was constructed and used for hemolysis assay and western blot to test the ability of MXSGT to inhibit PLY activity, production and widowed characteristics. The growth curve of S. pneumoniae was drawled with or without MXSGT treatment. In addition, the inhibition of MXSGT against PLY-mediated A549 cell death was examined by cytotoxicity assay. Finally, the mouse experiment was used to verify the effect of MXSGT on mouse lungs.
    Results: This work has discovered that MXSGT, a TCM prescription, is an effective inhibitor of PLY, an important virulence factor that is essential for S. pneumoniae pathogenicity. MXSGT inhibits the oligomerization of PLY without affecting S. pneumoniae growth and PLY production. In addition, experimental MXSGT treatment was effective against S. pneumoniae infection both in vitro and in vivo.
    Conclusion: These findings directly demonstrate the potential mechanism of the Chinese herbal formula MXSGT in the treatment of pneumococcal disease and provide additional evidence for promotion of the wide use of MXSGT in the clinic.
    MeSH term(s) A549 Cells ; Animals ; Anti-Bacterial Agents/chemistry ; Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/therapeutic use ; Bacterial Proteins/antagonists & inhibitors ; Bacterial Proteins/metabolism ; Disease Models, Animal ; Drugs, Chinese Herbal/chemistry ; Drugs, Chinese Herbal/pharmacology ; Drugs, Chinese Herbal/therapeutic use ; Hemolysis/drug effects ; Humans ; Lung/drug effects ; Lung/pathology ; Medicine, Chinese Traditional ; Mice, Inbred BALB C ; Sheep ; Streptococcus pneumoniae/drug effects ; Streptococcus pneumoniae/pathogenicity ; Streptolysins/antagonists & inhibitors ; Streptolysins/metabolism ; Virulence/drug effects ; Mice
    Chemical Substances Anti-Bacterial Agents ; Bacterial Proteins ; Drugs, Chinese Herbal ; Streptolysins ; ma-xing-shi-gan-tang ; plY protein, Streptococcus pneumoniae
    Language English
    Publishing date 2021-04-20
    Publishing country Ireland
    Document type Journal Article
    ZDB-ID 134511-4
    ISSN 1872-7573 ; 0378-8741
    ISSN (online) 1872-7573
    ISSN 0378-8741
    DOI 10.1016/j.jep.2021.114133
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Nomenclatural changes in the genus Bambusananus Li & Xing, 2011 (Hemiptera: Cicadellidae: Deltocephalinae: Athysanini).

    Xing, Ji-chun / Chen, Xiang-sheng

    Zootaxa

    2015  Volume 3635, Page(s) 599–600

    MeSH term(s) Animals ; Hemiptera/classification ; Species Specificity ; Terminology as Topic
    Language English
    Publishing date 2015-06-02
    Publishing country New Zealand
    Document type Letter ; Research Support, Non-U.S. Gov't
    ISSN 1175-5326
    ISSN 1175-5326
    DOI 10.11646/zootaxa.3635.5.12
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Deciphering the Pharmacological Mechanisms of Ma Xing Shi Gan Decoction against COVID-19 through Integrating Network Pharmacology and Experimental Exploration

    Qianqian Li / Chen Bai / Ruocong Yang / Weiying Xing / Xiaohan Pang / Siying Wu / Shaoyang Liu / Jianxin Chen / Tiegang Liu / Xiaohong Gu

    Frontiers in Pharmacology, Vol

    2020  Volume 11

    Abstract: ... viral pneumonia. In this study, we revealed the efficacy and pharmacological mechanism of Ma Xing Shi Gan (MXSG ...

    Abstract The outbreak of new infectious pneumonia caused by SARS-CoV-2 has posed a significant threat to public health, but specific medicines and vaccines are still being developed. Traditional Chinese medicine (TCM) has thousands of years of experience in facing the epidemic disease, such as influenza and viral pneumonia. In this study, we revealed the efficacy and pharmacological mechanism of Ma Xing Shi Gan (MXSG) Decoction against COVID-19. First, we used liquid chromatography–electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) to analyze the chemical components in MXSG and identified a total of 97 components from MXSG. Then, the intervention pathway of MXSG based on these components was analyzed with network pharmacology, and it was found that the pathways related to the virus infection process were enriched in some of MXSG component targets. Simultaneously, through literature research, it was preliminarily determined that MXSG, which is an essential prescription for treating COVID-19, shared the feature of antiviral, improving clinical symptoms, regulating immune inflammation, and inhibiting lung injury. The regulatory mechanisms associated with its treatment of COVID-19 were proposed. That MXSG might directly inhibit the adsorption and replication of SARS-CoV-2 at the viral entry step. Besides, MXSG might play a critical role in inflammation and immune regulatory, that is, to prevent cytokine storm and relieve lung injury through toll-like receptors signaling pathway. Next, in this study, the regulatory effect of MXSG on inflammatory lung injury was validated through transcriptome results. In summary, MXSG is a relatively active and safe treatment for influenza and viral pneumonia, and its therapeutic effect may be attributed to its antiviral and anti-inflammatory effects.
    Keywords Ma Xing Shi Gan decoction ; COVID-19 ; Traditional Chinese Medicine ; immunomodulatory ; antiviral ; Therapeutics. Pharmacology ; RM1-950
    Subject code 570
    Language English
    Publishing date 2020-11-01T00:00:00Z
    Publisher Frontiers Media S.A.
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article: The TCM prescription Ma-xing-shi-gan-tang inhibits Streptococcus pneumoniae pathogenesis by targeting pneumolysin

    Guo, Tingting / Guo, Yinan / Liu, Qingbing / Xu, Yan / Wei, Lina / Wang, Zhongtian / Chen, Si / Wang, Caiwen / Tian, Ye / Cui, Jie / Wang, Yijie / Wang, Yanbo / Sun, Liping

    Journal of ethnopharmacology. 2021 July 15, v. 275

    2021  

    Abstract: Ma-xing-shi-gan-tang (MXSGT), which is documented in the Treatise on Febrile Diseases and is ...

    Abstract Ma-xing-shi-gan-tang (MXSGT), which is documented in the Treatise on Febrile Diseases and is a therapeutic drug, is a well-known classic prescription in China and has been widely studied. Previous studies have shown that MXSGT has various pharmacological activities, including anti-influenza virus activity, and ameliorates microvascular hyperpermeability and inflammatory reactions. However, no study has reported the effect of MXSGT in the treatment of bacterial pneumonia.In this study, the potential inhibition of MXSGT against the virulence of S. pneumoniae by targeting PLY was investigated.First, HPLC analysis was used to determine the main components of MXSGT. Then PLY protein was constructed and used for hemolysis assay and western blot to test the ability of MXSGT to inhibit PLY activity, production and widowed characteristics. The growth curve of S. pneumoniae was drawled with or without MXSGT treatment. In addition, the inhibition of MXSGT against PLY-mediated A549 cell death was examined by cytotoxicity assay. Finally, the mouse experiment was used to verify the effect of MXSGT on mouse lungs.This work has discovered that MXSGT, a TCM prescription, is an effective inhibitor of PLY, an important virulence factor that is essential for S. pneumoniae pathogenicity. MXSGT inhibits the oligomerization of PLY without affecting S. pneumoniae growth and PLY production. In addition, experimental MXSGT treatment was effective against S. pneumoniae infection both in vitro and in vivo.These findings directly demonstrate the potential mechanism of the Chinese herbal formula MXSGT in the treatment of pneumococcal disease and provide additional evidence for promotion of the wide use of MXSGT in the clinic.
    Keywords Streptococcus pneumoniae ; Western blotting ; antiviral properties ; cell death ; drugs ; growth curves ; hemolysis ; mice ; oligomerization ; pathogenesis ; pneumolysin ; therapeutics ; toxicity testing ; traditional medicine ; virulence ; China
    Language English
    Dates of publication 2021-0715
    Publishing place Elsevier B.V.
    Document type Article
    Note NAL-AP-2-clean
    ZDB-ID 134511-4
    ISSN 1872-7573 ; 0378-8741
    ISSN (online) 1872-7573
    ISSN 0378-8741
    DOI 10.1016/j.jep.2021.114133
    Database NAL-Catalogue (AGRICOLA)

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  7. Article ; Online: Systems pharmacology dissection of the anti-stroke mechanism for the Chinese traditional medicine Xing-Nao-Jing.

    Chen, Yuhua / Sun, Yue / Li, Wende / Wei, Hong / Long, Tianlin / Li, Hua / Xu, Quanhua / Liu, Wei

    Journal of pharmacological sciences

    2018  Volume 136, Issue 1, Page(s) 16–25

    Abstract: Xing-Nao-Jing (XNJ) is a well-known injection that has been extensively applied in clinical ...

    Abstract Xing-Nao-Jing (XNJ) is a well-known injection that has been extensively applied in clinical treatment of stroke in China. However, the underlying mechanism of clinical administration of XNJ in stroke remains unclear. In this study, a systems pharmacology strategy based on pharmacokinetic and pharmacodynamics data was applied to analyze the pharmacological effect of XNJ on stroke. Sixteen active compounds were filtered from XNJ through Drug-likeness (DL) and Brain-blood-barrier (BBB) evaluations. Ninety-four potential targets of these active components were identified by SysDT and SEA. Biological process and pathway enrichment analyses of these targets demonstrated that XNJ exerted anti-stroke effects by biological processes and pathways, such as the response to oxidative stress, regulation of blood pressure, calcium signaling pathway, and apoptosis. Integrating the compound-target network and stroke-related PPI network, we found that Akt1, HIF-1α and ITGB2 may play key roles in the treatment of stroke. The experiments demonstrated that oxycurcumenol may prevent PC12 cells from oxidative stress-induced cell damage. Our study indicates that XNJ has an effect on stroke by protecting neuro cells from oxidative stress-induced cell damage via HIF1α, and the research strategy at the systems pharmacology level is feasible to reveal the mechanisms of novel lead compounds from natural products.
    MeSH term(s) Animals ; Drugs, Chinese Herbal/pharmacokinetics ; Drugs, Chinese Herbal/pharmacology ; Hypoxia-Inducible Factor 1, alpha Subunit/physiology ; Oxidative Stress/drug effects ; PC12 Cells ; Phytotherapy ; Rats ; Sesquiterpenes/pharmacology ; Sesquiterpenes/therapeutic use ; Stroke/drug therapy ; Stroke/genetics ; Structure-Activity Relationship
    Chemical Substances Drugs, Chinese Herbal ; Hif1a protein, rat ; Hypoxia-Inducible Factor 1, alpha Subunit ; Sesquiterpenes ; curcumenol ; oxycurcumenol
    Language English
    Publishing date 2018-01
    Publishing country Japan
    Document type Journal Article
    ZDB-ID 2104264-0
    ISSN 1347-8648 ; 1347-8613
    ISSN (online) 1347-8648
    ISSN 1347-8613
    DOI 10.1016/j.jphs.2017.11.005
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Chemical composition and pharmacological mechanism of Qingfei Paidu Decoction and Ma Xing Shi Gan Decoction against Coronavirus Disease 2019 (COVID-19): In silico and experimental study.

    Yang, Ruocong / Liu, Hao / Bai, Chen / Wang, Yingchao / Zhang, Xiaohui / Guo, Rui / Wu, Siying / Wang, Jianxun / Leung, Elaine / Chang, Hang / Li, Peng / Liu, Tiegang / Wang, Yi

    Pharmacological research

    2020  Volume 157, Page(s) 104820

    Abstract: ... collecting the targets of identified compounds, indicated a pivotal role of Ma Xing Shi Gan Decoction (MXSG ...

    Abstract The Coronavirus Disease 2019 (COVID-19) pandemic has become a huge threaten to global health, which raise urgent demand of developing efficient therapeutic strategy. The aim of the present study is to dissect the chemical composition and the pharmacological mechanism of Qingfei Paidu Decoction (QFPD), a clinically used Chinese medicine for treating COVID-19 patients in China. Through comprehensive analysis by liquid chromatography coupled with high resolution mass spectrometry (MS), a total of 129 compounds of QFPD were putatively identified. We also constructed molecular networking of mass spectrometry data to classify these compounds into 14 main clusters, in which exhibited specific patterns of flavonoids (45 %), glycosides (15 %), carboxylic acids (10 %), and saponins (5 %). The target network model of QFPD, established by predicting and collecting the targets of identified compounds, indicated a pivotal role of Ma Xing Shi Gan Decoction (MXSG) in the therapeutic efficacy of QFPD. Supportively, through transcriptomic analysis of gene expression after MXSG administration in rat model of LPS-induced pneumonia, the thrombin and Toll-like receptor (TLR) signaling pathway were suggested to be essential pathways for MXSG mediated anti-inflammatory effects. Besides, changes in content of major compounds in MXSG during decoction were found by the chemical analysis. We also validate that one major compound in MXSG, i.e. glycyrrhizic acid, inhibited TLR agonists induced IL-6 production in macrophage. In conclusion, the integration of in silico and experimental results indicated that the therapeutic effects of QFPD against COVID-19 may be attributed to the anti-inflammatory effects of MXSG, which supports the rationality of the compatibility of TCM.
    MeSH term(s) Animals ; Anti-Inflammatory Agents/analysis ; Anti-Inflammatory Agents/pharmacology ; Betacoronavirus/drug effects ; COVID-19 ; Cells, Cultured ; Computer Simulation ; Coronavirus Infections/drug therapy ; Coronavirus Infections/genetics ; Drugs, Chinese Herbal/analysis ; Drugs, Chinese Herbal/pharmacology ; Drugs, Chinese Herbal/therapeutic use ; Gene Expression/drug effects ; Glycyrrhizic Acid/pharmacology ; Humans ; Interleukin-6/metabolism ; Lipopeptides/antagonists & inhibitors ; Lipopeptides/pharmacology ; Lipopolysaccharides ; Male ; Pandemics ; Pneumonia/chemically induced ; Pneumonia/metabolism ; Pneumonia, Viral/drug therapy ; Pneumonia, Viral/genetics ; Rats ; SARS-CoV-2 ; Signal Transduction/drug effects ; Thrombin/metabolism ; Toll-Like Receptors/metabolism
    Chemical Substances Anti-Inflammatory Agents ; Drugs, Chinese Herbal ; Interleukin-6 ; Lipopeptides ; Lipopolysaccharides ; Pam(3)CSK(4) peptide ; Toll-Like Receptors ; maxingshigan ; qingfei paidu decoction ; Glycyrrhizic Acid (6FO62043WK) ; Thrombin (EC 3.4.21.5)
    Keywords covid19
    Language English
    Publishing date 2020-04-29
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1003347-6
    ISSN 1096-1186 ; 0031-6989 ; 1043-6618
    ISSN (online) 1096-1186
    ISSN 0031-6989 ; 1043-6618
    DOI 10.1016/j.phrs.2020.104820
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: [Responses of Pinus sylvestris var. mongolica radial growth to climate warming in Great Xing' an Mountins: a case study in Mangui].

    Zhang, Xing-Liang / He, Xing-Yuan / Chen, Zhen-Ju / Cui, Ming-Xing / Li, Na

    Ying yong sheng tai xue bao = The journal of applied ecology

    2011  Volume 22, Issue 12, Page(s) 3101–3108

    Abstract: ... of Pinus sylvestris var. mongolica in Mangui of Great Xing' an Mountains was developed, and the relationships between ...

    Abstract Based on the theory and methodology of dendrochronology, the tree ring width chronology of Pinus sylvestris var. mongolica in Mangui of Great Xing' an Mountains was developed, and the relationships between the standardized tree ring width chronology and local climate factors (temperature and precipitation) as well as the effects of climate factors on the P. sylvestris var. mongolica radial growth were analyzed. In this region, the mean monthly temperature in April-August of current year was the main factor limiting the radial growth, and the increasing mean monthly temperature from April to August had negative effects to the radial growth. The simulation of the variations of the radial growth by the mean monthly temperature change in April-August showed that the radial growth of P. sylvestris var. mongolica would present a declining trend accompanied with the warmer and drier regional climate condition.
    MeSH term(s) China ; Chronobiology Phenomena ; Climate Change ; Conservation of Natural Resources ; Ecosystem ; Global Warming ; Pinus sylvestris/growth & development ; Plant Stems/anatomy & histology ; Plant Stems/growth & development
    Language Chinese
    Publishing date 2011-12
    Publishing country China
    Document type English Abstract ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2881809-X
    ISSN 1001-9332
    ISSN 1001-9332
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Chemical composition and pharmacological mechanism of Qingfei Paidu Decoction and Ma Xing Shi Gan Decoction against Coronavirus Disease 2019 (COVID-19)

    Yang, Ruocong / Liu, Hao / Bai, Chen / Wang, Yingchao / Zhang, Xiaohui / Guo, Rui / Wu, Siying / Wang, Jianxun / Leung, Elaine / Chang, Hang / Li, Peng / Liu, Tiegang / Wang, Yi

    Pharmacological Research

    In silico and experimental study

    2020  Volume 157, Page(s) 104820

    Keywords Pharmacology ; covid19
    Language English
    Publisher Elsevier BV
    Publishing country us
    Document type Article ; Online
    ISSN 1043-6618
    DOI 10.1016/j.phrs.2020.104820
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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