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Article ; Online: Characterization of Flavor Profiles of Cigar Tobacco Leaves Grown in China via Headspace-Gas Chromatography-Ion Mobility Spectrometry Coupled with Multivariate Analysis and Sensory Evaluation.

Zhu, Beibei / An, Hongyue / Li, Li / Zhang, Hongfei / Lv, Jinxiong / Hu, Wanrong / Xue, Fang / Liu, Lulu / He, Shengbao / Li, Dongliang

ACS omega

2024 Volume 9, Issue 14, Page(s) 15996–16005

Abstract: Although cigar tobacco leaves (CTLs) have a high economic value, research regarding the flavor characteristics of CTLs is currently limited. A comprehensive study of the flavor characteristics of CTLs from different regions of China was conducted by ... ...

Abstract	Although cigar tobacco leaves (CTLs) have a high economic value, research regarding the flavor characteristics of CTLs is currently limited. A comprehensive study of the flavor characteristics of CTLs from different regions of China was conducted by identifying their volatile-flavor-containing compounds (VFCs) and flavors. The samples were analyzed via gas chromatography-ion mobility spectrometry (GC-IMS) and sensory evaluation. Results revealed considerable differences in the VFC contents of CTLs from different regions of China, suggesting that the VFLs of CTLs could be influenced by geographical origin. Mainly, phenols, pyrazines, and aldehydes were present in the CTLs from Sichuan. High contents of esters and pyrazines were present in the CTLs from Hubei, while esters were the major components of the CTLs from Hainan. Multivariate analysis results showed the effective differentiation of samples from different geographical origins based on the GC-IMS results. Sensory evaluation revealed that the flavors of CTLs from different geographical origins were different. 1,8-Pinene, 3-methyl-3-butene-1-ol, 2,3-dimethyl-5-ethylpyrazine, 4-methyl-3-penten-2-one, and (
Language	English
Publishing date	2024-03-26
Publishing country	United States
Document type	Journal Article
ISSN	2470-1343
ISSN (online)	2470-1343
DOI	10.1021/acsomega.3c09499
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Controllable Synthesis of Transferable Ultrathin Bi

Chen, Jiabiao / Liu, Zhaochao / Lv, Zunxian / Hou, Yameng / Chen, Xiang / Lan, Lan / Cheng, Tong-Huai / Zhang, Lei / Duan, Yingnan / Fu, Huixia / Fu, Xuewen / Luo, Feng / Wu, Jinxiong

Journal of the American Chemical Society

2024

Abstract: Two-dimensional (2D) alloys hold great promise to serve as important components of 2D transistors, since their properties allow continuous regulation by varying their compositions. However, previous studies are mainly limited to the metallic/ ... ...

Abstract	Two-dimensional (2D) alloys hold great promise to serve as important components of 2D transistors, since their properties allow continuous regulation by varying their compositions. However, previous studies are mainly limited to the metallic/semiconducting ones as contact/channel materials, but very few are related to the insulating dielectrics. Here, we use a facile one-step chemical vapor deposition (CVD) method to synthesize ultrathin Bi
Language	English
Publishing date	2024-04-14
Publishing country	United States
Document type	Journal Article
ZDB-ID	3155-0
ISSN	1520-5126 ; 0002-7863
ISSN (online)	1520-5126
ISSN	0002-7863
DOI	10.1021/jacs.4c02496
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Article ; Online: Detection of Phenotype-Related Mutations of COVID-19 via the Whole Genomic Data.

Lv, Jinxiong / Tu, Shikui / Xu, Lei

IEEE/ACM transactions on computational biology and bioinformatics

2021 Volume 18, Issue 4, Page(s) 1242–1249

Abstract: The coronavirus disease 2019 (COVID-19) epidemic continues to spread rapidly around the world and nearly 20 millions people are infected. This paper utilises both single-locus analysis and joint-SNPs analysis for detection of significant single ... ...

Abstract	The coronavirus disease 2019 (COVID-19) epidemic continues to spread rapidly around the world and nearly 20 millions people are infected. This paper utilises both single-locus analysis and joint-SNPs analysis for detection of significant single nucleotide polymorphisms (SNPs) in the phenotypes of symptomatic versus asymptomatic, the early collection time versus the late collection time, the old versus the young, and the male versus the female. Also, this paper analyses the relationship between any two SNPs via linkage disequilibrium analysis, and visualises the patterns of cumulative mutations of SNPs over collection time. The results are in three folds. First, the SNP which locates at the nucleotide position 4321 is found to be an independent significant locus associated with all the first three phenotypes. Moreover, 12 significant SNPs are found in the first two studies. Second, gene orf1ab containing SNP-4321 is detected to be significantly associated with the first three phenotypes, and the three genes S, ORF3a, and N, are detected to be significant in the first two phenotypes. Third, some of the detected genes or SNPs are related to the SARS-COV-2 as supported by literature survey, which indicates that the results here may be helpful for further investigation.
MeSH term(s)	COVID-19/epidemiology ; COVID-19/virology ; Computational Biology ; Databases, Genetic ; Female ; Genome, Viral ; Genome-Wide Association Study ; Humans ; Japan/epidemiology ; Linkage Disequilibrium ; Male ; Middle Aged ; Mutation ; Pandemics ; Phenotype ; Polymorphism, Single Nucleotide ; SARS-CoV-2/genetics ; Whole Genome Sequencing
Language	English
Publishing date	2021-08-06
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1557-9964
ISSN (online)	1557-9964
DOI	10.1109/TCBB.2021.3049836
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Article: Genetic and Comparative Transcriptome Analysis Revealed DEGs Involved in the Purple Leaf Formation in

Heng, Shuangping / Wang, Lei / Yang, Xi / Huang, Hao / Chen, Guo / Cui, Mengdi / Liu, Mingfang / Lv, Qing / Wan, Zhengjie / Shen, Jinxiong / Fu, Tingdong

Frontiers in genetics

2020 Volume 11, Page(s) 322

Abstract: ... Brassica ... ...

Abstract	Brassica juncea
Language	English
Publishing date	2020-04-24
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2606823-0
ISSN	1664-8021
ISSN	1664-8021
DOI	10.3389/fgene.2020.00322
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Article: Increased seed number per silique in Brassica juncea by deleting cis‐regulatory region affecting BjCLV1 expression in carpel margin meristem

Wang, Gang / Zhang, Xiangxiang / Huang, Wei / Xu, Ping / Lv, Zewen / Zhao, Lun / Wen, Jing / Yi, Bin / Ma, Chaozhi / Tu, Jinxing / Fu, Tingdong / Shen, Jinxiong

Plant biotechnology journal. 2021 Nov., v. 19, no. 11

2021

Abstract: Mustard yield per plant is severely restricted by the seed number per silique. The seed number per silique in the Brassica juncea trilocular mutant J163‐4 is significantly greater than that in normal bilocular plants. However, how the trilocular silique ... ...

Abstract	Mustard yield per plant is severely restricted by the seed number per silique. The seed number per silique in the Brassica juncea trilocular mutant J163‐4 is significantly greater than that in normal bilocular plants. However, how the trilocular silique of J163‐4 is formed remains unclear. Here, we studied the gene structure and function of mc2 in B. juncea and Arabidopsis using comparative morphology and molecular genetic experiments. We found that mc2 is a CLV1 ortholog, BjA7.CLV1. The deletion of cis‐regulatory region in mc2 promoter, which affects Mc2 expression in carpel margin meristem (CMM), led to trilocular silique formation. The BjCLV1 sequence with its complete promoter containing the cis‐regulatory region can restore the Bjclv1 and clv1 mutant phenotypes in B. juncea and Arabidopsis, respectively. Additionally, this cis‐regulatory region had a collinear segment in the promoter of CLV1 homologous gene in most Brassicaceae species. Our results are consistent with the report that BjCLV1 represents a conserved pleiotropic role in shoot meristem and CMM development, which contains a cis‐regulatory sequence specifically expressed BjCLV1 in CMM in its promoter, and this cis‐regulatory region is conserved in Brassicaceae species. These results offer a reliable approach for fine‐tuning the traits of seed yield in Brassicaceae crops.
Keywords	Arabidopsis ; Brassica juncea ; biotechnology ; genes ; mutants ; seed yield ; shoot meristems ; siliques
Language	English
Dates of publication	2021-11
Size	p. 2333-2348.
Publishing place	John Wiley & Sons, Ltd
Document type	Article
Note	JOURNAL ARTICLE
ZDB-ID	2136367-5
ISSN	1467-7652 ; 1467-7644
ISSN (online)	1467-7652
ISSN	1467-7644
DOI	10.1111/pbi.13664
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Increased seed number per silique in Brassica juncea by deleting cis-regulatory region affecting BjCLV1 expression in carpel margin meristem.

Wang, Gang / Zhang, Xiangxiang / Huang, Wei / Xu, Ping / Lv, Zewen / Zhao, Lun / Wen, Jing / Yi, Bin / Ma, Chaozhi / Tu, Jinxing / Fu, Tingdong / Shen, Jinxiong

Plant biotechnology journal

2021 Volume 19, Issue 11, Page(s) 2333–2348

Abstract: Mustard yield per plant is severely restricted by the seed number per silique. The seed number per silique in the Brassica juncea trilocular mutant J163-4 is significantly greater than that in normal bilocular plants. However, how the trilocular silique ... ...

Abstract	Mustard yield per plant is severely restricted by the seed number per silique. The seed number per silique in the Brassica juncea trilocular mutant J163-4 is significantly greater than that in normal bilocular plants. However, how the trilocular silique of J163-4 is formed remains unclear. Here, we studied the gene structure and function of mc2 in B. juncea and Arabidopsis using comparative morphology and molecular genetic experiments. We found that mc2 is a CLV1 ortholog, BjA7.CLV1. The deletion of cis-regulatory region in mc2 promoter, which affects Mc2 expression in carpel margin meristem (CMM), led to trilocular silique formation. The BjCLV1 sequence with its complete promoter containing the cis-regulatory region can restore the Bjclv1 and clv1 mutant phenotypes in B. juncea and Arabidopsis, respectively. Additionally, this cis-regulatory region had a collinear segment in the promoter of CLV1 homologous gene in most Brassicaceae species. Our results are consistent with the report that BjCLV1 represents a conserved pleiotropic role in shoot meristem and CMM development, which contains a cis-regulatory sequence specifically expressed BjCLV1 in CMM in its promoter, and this cis-regulatory region is conserved in Brassicaceae species. These results offer a reliable approach for fine-tuning the traits of seed yield in Brassicaceae crops.
MeSH term(s)	Arabidopsis/genetics ; Gene Expression Regulation, Plant/genetics ; Meristem/genetics ; Mustard Plant/genetics ; Promoter Regions, Genetic/genetics ; Seeds/genetics
Language	English
Publishing date	2021-08-31
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2136367-5
ISSN	1467-7652 ; 1467-7652
ISSN (online)	1467-7652
ISSN	1467-7652
DOI	10.1111/pbi.13664
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Article ; Online: Trilocular phenotype in Brassica juncea L. resulted from interruption of CLAVATA1 gene homologue (BjMc1) transcription.

Xu, Ping / Cao, Shiqin / Hu, Kaining / Wang, Xiaohua / Huang, Wei / Wang, Gang / Lv, Zewen / Liu, Zhongsong / Wen, Jing / Yi, Bin / Ma, Chaozhi / Tu, Jinxing / Fu, Tingdong / Shen, Jinxiong

Scientific reports

2017 Volume 7, Issue 1, Page(s) 3498

Abstract: As a desirable agricultural trait, multilocular trait of rapeseed (Brassica rapa; Brassica napus; Brassica juncea), always represents higher yield per plant compared with bilocular plants. We previously isolated a trilocular gene locus, Bjmc1, and ... ...

Abstract	As a desirable agricultural trait, multilocular trait of rapeseed (Brassica rapa; Brassica napus; Brassica juncea), always represents higher yield per plant compared with bilocular plants. We previously isolated a trilocular gene locus, Bjmc1, and identified a set of molecular markers linked to the trilocular gene. With a map-based cloning, we identified that the BjMc1 was located in B genome of Brassica juncea, and it was a CLAVATA1 (CLV1) gene homologue. The insertion of a copia-LTR retrotransposable element 1 (RTE1) into the coding region of BjMc1 interrupted its transcription in rapeseed, leading to the trilocular phenotype. Phylogenetic analysis showed that Mc1 genes were conserved and widespread in land plants. Two amino acid sites had undergone positive selection in the ancestor of Mc1 genes, and then purifying selection was the dominant force after the divergence of dicots and monocots from their common ancestor in the evolutionary process, indicating that Mc1 genes are conserved in modern land plants. Our results provided new insights in molecular regulatory mechanism of multilocularity in rapeseed, and better understanding of molecular mechanism in crop yield improvement.
MeSH term(s)	Arabidopsis Proteins/genetics ; Biological Evolution ; Caspases/genetics ; Chromosome Mapping ; Genes, Plant ; Mustard Plant/genetics ; Phenotype ; Protein-Serine-Threonine Kinases/genetics
Chemical Substances	Arabidopsis Proteins ; CLV1 protein, Arabidopsis (EC 2.7.11.1) ; Protein-Serine-Threonine Kinases (EC 2.7.11.1) ; Caspases (EC 3.4.22.-) ; MC1 protein, Arabidopsis (EC 3.4.22.-)
Language	English
Publishing date	2017-06-14
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-017-03755-0
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Article ; Online: Trilocular phenotype in Brassica juncea L. resulted from interruption of CLAVATA1 gene homologue (BjMc1) transcription

Ping Xu / Shiqin Cao / Kaining Hu / Xiaohua Wang / Wei Huang / Gang Wang / Zewen Lv / Zhongsong Liu / Jing Wen / Bin Yi / Chaozhi Ma / Jinxing Tu / Tingdong Fu / Jinxiong Shen

Scientific Reports, Vol 7, Iss 1, Pp 1-

2017 Volume 12

Abstract: Abstract As a desirable agricultural trait, multilocular trait of rapeseed (Brassica rapa; Brassica napus; Brassica juncea), always represents higher yield per plant compared with bilocular plants. We previously isolated a trilocular gene locus, Bjmc1, ... ...

Abstract	Abstract As a desirable agricultural trait, multilocular trait of rapeseed (Brassica rapa; Brassica napus; Brassica juncea), always represents higher yield per plant compared with bilocular plants. We previously isolated a trilocular gene locus, Bjmc1, and identified a set of molecular markers linked to the trilocular gene. With a map-based cloning, we identified that the BjMc1 was located in B genome of Brassica juncea, and it was a CLAVATA1 (CLV1) gene homologue. The insertion of a copia-LTR retrotransposable element 1 (RTE1) into the coding region of BjMc1 interrupted its transcription in rapeseed, leading to the trilocular phenotype. Phylogenetic analysis showed that Mc1 genes were conserved and widespread in land plants. Two amino acid sites had undergone positive selection in the ancestor of Mc1 genes, and then purifying selection was the dominant force after the divergence of dicots and monocots from their common ancestor in the evolutionary process, indicating that Mc1 genes are conserved in modern land plants. Our results provided new insights in molecular regulatory mechanism of multilocularity in rapeseed, and better understanding of molecular mechanism in crop yield improvement.
Keywords	Medicine ; R ; Science ; Q
Subject code	580
Language	English
Publishing date	2017-06-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: OsABCG15 encodes a membrane protein that plays an important role in anther cuticle and pollen exine formation in rice

Wu, Lina / Guan, Yusheng / Wu, Zigang / Yang, Kun / Lv, Jun / Converse, Richard / Huang, Yuanxin / Mao, Jinxiong / Zhao, Yong / Wang, Zhongwei / Min, Hengqi / Kan, Dongyang / Zhang, Yi

Plant cell reports. 2014 Nov., v. 33, no. 11

2014

Abstract: KEY MESSAGE : An ABC transporter gene ( OsABCG15 ) was proven to be involved in pollen development in rice. The corresponding protein was localized on the plasma membrane using subcellular localization. Wax, cutin, and sporopollenin are important for ... ...

Abstract	KEY MESSAGE : An ABC transporter gene ( OsABCG15 ) was proven to be involved in pollen development in rice. The corresponding protein was localized on the plasma membrane using subcellular localization. Wax, cutin, and sporopollenin are important for normal development of the anther cuticle and pollen exine, respectively. Their lipid soluble precursors, which are produced in the tapetum, are then secreted and transferred to the anther and microspore surface for polymerization. However, little is known about the mechanisms underlying the transport of these precursors. Here, we identified and characterized a member of the G subfamily of ATP-binding cassette (ABC) transporters, OsABCG15, which is required for the secretion of these lipid-soluble precursors in rice. Using map-based cloning, we found a spontaneous A-to-C transition in the fourth exon of OsABCG15 that caused an amino acid substitution of Thr-to-Pro in the predicted ATP-binding domain of the protein sequence. This osabcg15 mutant failed to produce any viable pollen and was completely male sterile. Histological analysis indicated that osabcg15 exhibited an undeveloped anther cuticle, enlarged middle layer, abnormal Ubisch body development, tapetum degeneration with a falling apart style, and collapsed pollen grains without detectable exine. OsABCG15 was expressed preferentially in the tapetum, and the fused GFP-OsABCG15 protein was localized to the plasma membrane. Our results suggested that OsABCG15 played an essential role in the formation of the rice anther cuticle and pollen exine. This role may include the secretion of the lipid precursors from the tapetum to facilitate the transfer of precursors to the surface of the anther epidermis as well as to microspores.
Keywords	amino acid substitution ; cutin ; exine ; exons ; genes ; microspores ; molecular cloning ; mutants ; plasma membrane ; pollen ; polymerization ; rice ; secretion ; transporters
Language	English
Dates of publication	2014-11
Size	p. 1881-1899.
Publishing place	Springer-Verlag
Document type	Article
ZDB-ID	8397-5
ISSN	1432-203X ; 0721-085X ; 0721-7714
ISSN (online)	1432-203X
ISSN	0721-085X ; 0721-7714
DOI	10.1007/s00299-014-1666-8
Database	NAL-Catalogue (AGRICOLA)

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Article: Identification of molecular markers linked to trilocular gene (mc1) in Brassica juncea L

Xu, Ping / Lv, Zewen / Zhang, Xiangxiang / Wang, Xiaohua / Pu, Yuanyuan / Wang, Hongmei / Yi, Bin / Wen, Jing / Ma, Chaozhi / Tu, Jinxing / Fu, Tingdong / Shen, Jinxiong

Molecular breeding. 2014 Feb., v. 33, no. 2

2014

Abstract: Multilocular plants generally have higher yield than bilocular plants, which is attributable to the difference in the number of seeds per silique. In this study, we investigated a landrace of Brassica juncea in China that has trilocular siliques. We ... ...

Abstract	Multilocular plants generally have higher yield than bilocular plants, which is attributable to the difference in the number of seeds per silique. In this study, we investigated a landrace of Brassica juncea in China that has trilocular siliques. We discovered that two independent recessive genes (mc1 and mc2) controlled the trait of trilocular silique. Mc1 was preliminarily mapped using amplified fragment length polymorphism (AFLP) technology in combination with bulked segregant analysis. From a survey of 2,048 AFLP primer combinations, we obtained 24 AFLP markers linked to the target gene, of which five were successfully converted into sequence-characterized amplified region markers. Two of these five AFLP markers and two other AFLP markers shared high sequence homology with A07 in the Brassica rapa genome. We developed seven simple sequence repeat primer pairs based on their sequence homology with A07. Our result also demonstrated that EC14MC14 and SC20 are the closest markers flanking the Mc1 gene, at distances of 1.1 and 1.6� cM, respectively. These molecular markers will facilitate the fine mapping and cloning of the mc1 gene and accelerate the selection process for multilocular rapeseed through marker-assisted selection.
Keywords	Brassica juncea ; Brassica rapa ; amplified fragment length polymorphism ; fruits ; genetic markers ; landraces ; marker-assisted selection ; recessive genes ; seeds ; sequence homology ; surveys ; China
Language	English
Dates of publication	2014-02
Size	p. 425-434.
Publishing place	Springer-Verlag
Document type	Article
ZDB-ID	1230924-2
ISSN	1572-9788 ; 1380-3743
ISSN (online)	1572-9788
ISSN	1380-3743
DOI	10.1007/s11032-013-9960-7
Database	NAL-Catalogue (AGRICOLA)

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