LIVIVO - Search results -

Search results

Result 1 - 10 of total 14

Search options

Book ; Online ; Thesis: Untersuchung einer diskriminierenden quantitativen reverse-Transkriptase-Polymerase-Kettenreaktion zum Nachweis mutierter Coronaviren bei Katzen mit feliner infektiöser Peritonitis

Sangl, Laura [Verfasser] / Hartmann, Katrin [Akademischer Betreuer]

2020

Author's details	Laura Sangl ; Betreuer: Katrin Hartmann
Keywords	Tiere (Zoologie) ; Animals (Zoology)
Subject code	sg590
Language	German
Publisher	Universitätsbibliothek der Ludwig-Maximilians-Universität
Publishing place	München
Document type	Book ; Online ; Thesis
Database	Digital theses on the web

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Diagnostic Value of Detecting Feline Coronavirus RNA and Spike Gene Mutations in Cerebrospinal Fluid to Confirm Feline Infectious Peritonitis.

Felten, Sandra / Matiasek, Kaspar / Leutenegger, Christian M / Sangl, Laura / Herre, Stephanie / Dörfelt, Stefanie / Fischer, Andrea / Hartmann, Katrin

Viruses

2021 Volume 13, Issue 2

Abstract: Background: Cats with neurologic feline infectious peritonitis (FIP) are difficult to diagnose. Aim of this study was to evaluate the diagnostic value of detecting feline coronavirus (FCoV) RNA and spike (S) gene mutations in cerebrospinal fluid (CSF).!# ...

Abstract	Background: Cats with neurologic feline infectious peritonitis (FIP) are difficult to diagnose. Aim of this study was to evaluate the diagnostic value of detecting feline coronavirus (FCoV) RNA and spike (S) gene mutations in cerebrospinal fluid (CSF). Methods: The study included 30 cats with confirmed FIP (six with neurological signs) and 29 control cats (eleven with neurological signs) with other diseases resulting in similar clinical signs. CSF was tested for FCoV RNA by 7b-RT-qPCR in all cats. In RT-qPCR-positive cases, S-RT-qPCR was additionally performed to identify spike gene mutations. Results: Nine cats with FIP (9/30, 30%), but none of the control cats were positive for FCoV RNA in CSF. Sensitivity of 7b-RT-qPCR in CSF was higher for cats with neurological FIP (83.3%; 95% confidence interval (95% CI) 41.8-98.9) than for cats with non-neurological FIP (16.7%; 95% CI 6.1-36.5). Spike gene mutations were rarely detected. Conclusions: FCoV RNA was frequently present in CSF of cats with neurological FIP, but only rarely in cats with non-neurological FIP. Screening for spike gene mutations did not enhance specificity in this patient group. Larger populations of cats with neurological FIP should be explored in future studies.
MeSH term(s)	Animals ; Case-Control Studies ; Cats ; Coronavirus, Feline/genetics ; Coronavirus, Feline/isolation & purification ; Feline Infectious Peritonitis/cerebrospinal fluid ; Feline Infectious Peritonitis/diagnosis ; Feline Infectious Peritonitis/pathology ; Female ; Male ; Molecular Diagnostic Techniques/veterinary ; Mutation ; Prospective Studies ; RNA, Viral/cerebrospinal fluid ; RNA, Viral/genetics ; Real-Time Polymerase Chain Reaction/veterinary ; Sensitivity and Specificity ; Spike Glycoprotein, Coronavirus/genetics
Chemical Substances	RNA, Viral ; Spike Glycoprotein, Coronavirus ; spike protein, feline infectious peritonitis virus
Language	English
Publishing date	2021-01-27
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2516098-9
ISSN	1999-4915 ; 1999-4915
ISSN (online)	1999-4915
ISSN	1999-4915
DOI	10.3390/v13020186
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Article ; Online: Immunocytochemistry of mesenteric lymph node fine-needle aspirates in the diagnosis of feline infectious peritonitis.

Felten, Sandra / Hartmann, Katrin / Doerfelt, Stefanie / Sangl, Laura / Hirschberger, Johannes / Matiasek, Kaspar

Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc

2019 Volume 31, Issue 2, Page(s) 210–216

Abstract: Immunohistochemistry (IHC) of tissue samples is considered the gold standard for diagnosing feline infectious peritonitis (FIP), and, in cats without body cavity effusion, IHC is the only method available to establish definitive antemortem diagnosis. ... ...

Abstract	Immunohistochemistry (IHC) of tissue samples is considered the gold standard for diagnosing feline infectious peritonitis (FIP), and, in cats without body cavity effusion, IHC is the only method available to establish definitive antemortem diagnosis. However, IHC requires invasive tissue sample collection. We evaluated sensitivity and specificity of an immunocytochemical assay of fine-needle aspirates (FNAs) of mesenteric lymph nodes that can be obtained noninvasively by ultrasound-guided aspiration to diagnose FIP. FNAs of mesenteric lymph nodes were obtained postmortem from 41 cats suspected of having FIP based on clinical and/or laboratory findings. FIP was confirmed immunohistochemically in 30 cats. In the other 11 cats, a disease other than FIP, which explained the clinical signs, was diagnosed histopathologically. Immunocytochemistry (ICC) was performed as an avidin-biotin complex method using a monoclonal anti-FCoV IgG 2A. Sensitivity, specificity, negative and positive predictive values (NPV, PPV, respectively) including 95% confidence intervals (95% CIs) were determined. ICC was positive in 17 of 30 cats with FIP, but also in 1 of 11 control cats that was diagnosed with lymphoma. Sensitivity of ICC was 53% (95% CI: 34-72); specificity 91% (95% CI: 59-100); NPV 42% (95% CI: 22-63); and PPV 94% (95% CI: 71-100). In a lethal disease such as FIP, specificity is most important in order to avoid euthanasia of unaffected cats. Given that a false-positive result occurred and FIP was correctly detected in only approximately half of the cases of FIP, ICC of mesenteric lymph node FNA alone cannot reliably confirm or exclude FIP, but can be a helpful test in conjunction with other diagnostic measures.
MeSH term(s)	Animals ; Biopsy, Fine-Needle ; Cats ; Coronavirus, Feline ; Feline Infectious Peritonitis/diagnosis ; Feline Infectious Peritonitis/pathology ; Immunohistochemistry/veterinary ; Lymph Nodes/pathology ; Lymph Nodes/virology ; Sensitivity and Specificity
Keywords	covid19
Language	English
Publishing date	2019-01-29
Publishing country	United States
Document type	Journal Article
ZDB-ID	287603-6
ISSN	1943-4936 ; 1040-6387
ISSN (online)	1943-4936
ISSN	1040-6387
DOI	10.1177/1040638718825280
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 3645: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Antigens under cover-The preservation and demasking of selected antigens for successful poststaining immunocytochemistry of effusion, brain smears, and lymph node aspirates.

Dörfelt, Stefanie / Matiasek, Lara A / Felten, Sandra / Sangl, Laura / Hartmann, Katrin / Matiasek, Kaspar

Veterinary clinical pathology

2019 Volume 48 Suppl 1, Page(s) 98–107

Abstract: Background: In clinical cytology, the applicability of an ancillary test such as immunocytochemistry is too often limited by low sample volume, poor cell representation, and sample preservation. Diagnosticians often read Romanowsky-stained cytology, ... ...

Abstract	Background: In clinical cytology, the applicability of an ancillary test such as immunocytochemistry is too often limited by low sample volume, poor cell representation, and sample preservation. Diagnosticians often read Romanowsky-stained cytology, although specific techniques such as immunocytochemistry are often essential for a definitive diagnosis. Objectives: The goal of the present study aimed to investigate if immunocytochemistry on previously-stained cytologic specimens was possible. Different pretreatments were examined to determine which treatment preserved antigenicity best. Methods: One hundred and twenty-two impression smears and 64 fine-needle aspirate preparations of brain and lymph nodes were processed and evaluated microscopically. The impact of staining cytologic preparations with a modified Wright's stain, using a destaining method, performing a coverslipping and decoverslipping process, and subjecting smears to a microwave treatment (MWT) were examined for the immunolabeling of selected nuclear, cytoplasmic, and plasmalemmal antigens, as well as intracellular feline coronavirus (FCoV). Biotinylated secondary antibodies were used, and the bound primary antibody was visualized using an ABC amplification kit. Results: Cellular antigens were reliably detected with immunocytochemistry after smears were stained with a Romansky stain and were coverslipped early after staining and stayed coverslipped until immediately before immunolabeling. The staining intensity reached the same levels as that of the controls if the films underwent MWT in citrate buffer. In contrast, FCoV antigen detection was abolished after any physicochemical interference. Conclusions: Poststaining immunocytochemistry represents a practical tool for additional investigations on prestained cytologic specimens when searching for cellular antigens. Paired untreated samples should be kept in case the workup requires testing for more vulnerable viral antigens.
MeSH term(s)	Animals ; Antibodies/immunology ; Antigens, Nuclear/immunology ; Antigens, Viral/immunology ; Azure Stains ; Biopsy, Fine-Needle/veterinary ; Brain/pathology ; Cats ; Coloring Agents ; Coronavirus, Feline/immunology ; Cytodiagnosis/veterinary ; Cytoplasm/immunology ; Eosine Yellowish-(YS) ; Glial Fibrillary Acidic Protein/immunology ; Immunohistochemistry/veterinary ; Lymph Nodes/pathology ; Microwaves ; Sensitivity and Specificity ; Specimen Handling/veterinary ; Staining and Labeling/veterinary ; Swine
Chemical Substances	Antibodies ; Antigens, Nuclear ; Antigens, Viral ; Azure Stains ; Coloring Agents ; Glial Fibrillary Acidic Protein ; Romanowsky-Giemsa stain ; Eosine Yellowish-(YS) (TDQ283MPCW)
Keywords	covid19
Language	English
Publishing date	2019-02-25
Publishing country	United States
Document type	Comparative Study ; Journal Article
ZDB-ID	2114702-4
ISSN	1939-165X ; 0275-6382
ISSN (online)	1939-165X
ISSN	0275-6382
DOI	10.1111/vcp.12702
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

Order via subito

Details ▾
- Full text online
- Order with fees

Article ; Online: Utility of an immunocytochemical assay using aqueous humor in the diagnosis of feline infectious peritonitis.

Felten, Sandra / Matiasek, Kaspar / Gruendl, Stefanie / Sangl, Laura / Hartmann, Katrin

Veterinary ophthalmology

2017 Volume 21, Issue 1, Page(s) 27–34

Abstract: Objective: In cats suffering from feline infectious peritonitis (FIP) without effusion, antemortem diagnosis is challenging. Uveitis is common in these cats. It was the aim of this study to evaluate sensitivity and specificity of an immunocytochemical ... ...

Abstract	Objective: In cats suffering from feline infectious peritonitis (FIP) without effusion, antemortem diagnosis is challenging. Uveitis is common in these cats. It was the aim of this study to evaluate sensitivity and specificity of an immunocytochemical assay (ICC) in aqueous humor of cats suspected of having FIP. Animals studied: The study included 26 cats with immunohistochemically confirmed FIP and 12 control cats for which FIP was suspected due to similar clinical or laboratory changes, but which suffered from other diseases confirmed via histopathology. Procedures: All aqueous humor samples were collected postmortem by paracentesis. ICC was carried out as avidin-biotin complex method. Sensitivity, specificity, and the overall accuracy including 95% confidence intervals (95% CI) were calculated. Results: Immunocytochemistry was positive in 16 of 25 cats with FIP and 2 of 11 control cats (one cat with lymphoma, one with pulmonary adenocarcinoma). Aqueous humor samples of one cat with FIP and of one control cat were excluded from statistical analysis. Sensitivity was 64.0% (95% CI: 42.5-82.0); specificity 81.8% (95% CI: 48.2-97.7); and overall accuracy 69.4% (95% CI: 51.9-83.7). Conclusions: As false-positive results occurred and specificity is most important in the diagnosis of FIP, the diagnostic utility of ICC in aqueous humor is limited. Further studies are required to clarify the origin of false-positive ICC results.
MeSH term(s)	Animals ; Aqueous Humor/virology ; Cat Diseases/diagnosis ; Cat Diseases/virology ; Cats ; Coronavirus, Feline/immunology ; Coronavirus, Feline/isolation & purification ; Feline Infectious Peritonitis/diagnosis ; Feline Infectious Peritonitis/virology ; Female ; Immunohistochemistry/standards ; Immunohistochemistry/veterinary ; Male ; Sensitivity and Specificity
Keywords	covid19
Language	English
Publishing date	2017-05-10
Publishing country	England
Document type	Evaluation Study ; Journal Article
ZDB-ID	2011043-1
ISSN	1463-5224 ; 1463-5216
ISSN (online)	1463-5224
ISSN	1463-5216
DOI	10.1111/vop.12474
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

Order via subito

Details ▾
- Full text online
- Order with fees

Article ; Online: Detection of feline coronavirus RNA, spike gene mutations, and feline coronavirus antigen in macrophages in aqueous humor of cats in the diagnosis of feline infectious peritonitis.

Sangl, Laura / Felten, Sandra / Matiasek, Kaspar / Dörfelt, Stefanie / Bergmann, Michele / Balzer, Hans-Jörg / Pantchev, Nikola / Leutenegger, Christian / Hartmann, Katrin

Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc

2020 Volume 32, Issue 4, Page(s) 527–534

Abstract: Uveitis is common in cats, and is often a feature of feline infectious peritonitis (FIP). We evaluated 3 tools for detection of feline coronavirus (FCoV) in aqueous humor: 1) ... ...

Abstract	Uveitis is common in cats, and is often a feature of feline infectious peritonitis (FIP). We evaluated 3 tools for detection of feline coronavirus (FCoV) in aqueous humor: 1) a
MeSH term(s)	Animals ; Aqueous Humor/cytology ; Case-Control Studies ; Cats ; Coronavirus Infections/veterinary ; Coronavirus Infections/virology ; Coronavirus, Feline/genetics ; Coronavirus, Feline/isolation & purification ; Feline Infectious Peritonitis/diagnosis ; Feline Infectious Peritonitis/virology ; Immunohistochemistry ; Macrophages/virology ; Mutation ; RNA, Viral/genetics ; RNA, Viral/isolation & purification ; Sensitivity and Specificity
Chemical Substances	RNA, Viral
Keywords	covid19
Language	English
Publishing date	2020-06-09
Publishing country	United States
Document type	Journal Article
ZDB-ID	287603-6
ISSN	1943-4936 ; 1040-6387
ISSN (online)	1943-4936
ISSN	1040-6387
DOI	10.1177/1040638720927362
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 3645: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Detection of feline coronavirus RNA, spike gene mutations, and feline coronavirus antigen in macrophages in aqueous humor of cats in the diagnosis of feline infectious peritonitis

Sangl, Laura / Felten, Sandra / Matiasek, Kaspar / Dörfelt, Stefanie / Bergmann, Michele / Balzer, Hans-Jörg / Pantchev, Nikola / Leutenegger, Christian / Hartmann, Katrin

Journal of Veterinary Diagnostic Investigation. 2020 July, v. 32, no. 4 p.527-534

2020

Abstract: Uveitis is common in cats, and is often a feature of feline infectious peritonitis (FIP). We evaluated 3 tools for detection of feline coronavirus (FCoV) in aqueous humor: 1) a 7b gene reverse-transcription real-time PCR (7b-RT-rtPCR) assay to detect ... ...

Abstract	Uveitis is common in cats, and is often a feature of feline infectious peritonitis (FIP). We evaluated 3 tools for detection of feline coronavirus (FCoV) in aqueous humor: 1) a 7b gene reverse-transcription real-time PCR (7b-RT-rtPCR) assay to detect FCoV RNA, 2) a spike gene mutation RT-rtPCR (S-RT-rtPCR) assay to detect 2 point mutations in the spike gene of FCoV in cats positive by 7b-RT-rtPCR, and 3) immunocytochemistry (ICC) for detection of FCoV antigen in aqueous humor macrophages. We studied 58 cats, including 31 cats with FIP and 27 control cats. FIP was excluded by postmortem examination and negative immunohistochemistry (IHC). Aqueous humor samples obtained postmortem were assessed using 7b-RT-rtPCR in all cats, and positive samples were evaluated with S-RT-rtPCR. ICC evaluation of aqueous humor samples from 36 of the 58 cats was done using an avidin–biotin complex method and monoclonal anti-FCoV IgG 2A. Sensitivity, specificity, and negative and positive predictive values were calculated including 95% CIs. 7b-RT-rtPCR had a specificity of 100.0% (95% CI: 87.2–100.0) and sensitivity of 35.5% (95% CI: 19.2–54.6). Specificity of S-RT-rtPCR could not be determined because there were no FCoV 7b-RT-rtPCR–positive samples in the control group. Sensitivity of S-RT-rtPCR was 12.9% (95% CI 3.6–29.8). Sensitivity and specificity of ICC were 62.5% (95% CI: 40.6–81.2) and 80.0% (95% CI: 44.4–97.5), respectively. The combination of 7b-RT-rtPCR and IHC could be useful in diagnosing FIP; S-RT-rtPCR did not add value; and ICC of aqueous humor samples cannot be recommended for the diagnosis of FIP.
Keywords	Feline coronavirus ; RNA ; antigens ; feline infectious peritonitis ; genes ; immunocytochemistry ; immunohistochemistry ; macrophages ; mutation ; necropsy ; quantitative polymerase chain reaction ; reverse transcription ; 7b gene reverse-transcription real-time PCR ; aqueous humor ; cats ; spike gene mutation reverse-transcription real-time PCR ; uveitis
Language	English
Dates of publication	2020-07
Size	p. 527-534.
Publishing place	SAGE Publications
Document type	Article ; Online
Note	NAL-AP-2-clean
ZDB-ID	287603-6
ISSN	1943-4936 ; 1040-6387
ISSN (online)	1943-4936
ISSN	1040-6387
DOI	10.1177/1040638720927362
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Cologne/Königswinter

Zs.A 3645: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Investigation into the utility of an immunocytochemical assay in body cavity effusions for diagnosis of feline infectious peritonitis.

Felten, Sandra / Matiasek, Kaspar / Gruendl, Stefanie / Sangl, Laura / Wess, Gerhard / Hartmann, Katrin

Journal of feline medicine and surgery

2017 Volume 19, Issue 4, Page(s) 410–418

Abstract: Objectives Feline coronaviruses (FCoVs) exist as two biotypes, feline enteric coronavirus and feline infectious peritonitis virus. Although feline infectious peritonitis (FIP) is a very common disease, the ante-mortem diagnosis of this disease still ... ...

Abstract	Objectives Feline coronaviruses (FCoVs) exist as two biotypes, feline enteric coronavirus and feline infectious peritonitis virus. Although feline infectious peritonitis (FIP) is a very common disease, the ante-mortem diagnosis of this disease still remains a challenge. Immunofluorescence staining of FCoV in macrophages in effusion has been considered as the reference standard for the diagnosis, but recently this method has been shown to have lower specificity than previously reported. In addition, this method is not widely available and requires the use of fluorescence microscopes. Therefore, it was the aim of this study to evaluate the diagnostic potential of an immunocytochemical (ICC) assay using body cavity effusion. Methods Effusion samples from 27 cats with immunohistochemically confirmed FIP and 29 cats with suspected FIP but a definitive diagnosis of another disease were examined. ICC specimens were evaluated with respect to positive immunostaining. In addition, effusion samples were stained with haematoxylin and eosin and evaluated cytologically. Results A diagnostic sensitivity of 85.2% was recorded for effusion specimens (95% confidence interval [CI] 66.3-95.8), while the diagnostic specificity was only 72.4% (95% CI 52.8-87.3). Conclusions and relevance Once the clinical disease of FIP develops in a cat, it always leads to death, and most of the cats are euthanased within a few days or weeks. As false-positive results might lead to euthanasia of cats suffering from potentially treatable diseases, the diagnostic specificity of a diagnostic tool is the most important factor in a fatal disease like FIP. Thus, the diagnostic utility of this test proved to be insufficient and positive ICC results should be interpreted with caution. Nevertheless, full-body necropsy could not be performed in 13/29 control cats. It is possible that these cats actually suffered from early-stage FIP and that this fact might have influenced the diagnostic specificity of the ICC. Based on the results of the present study, however, ICC of effusion samples currently cannot be recommended to confirm a suspicion of FIP.
MeSH term(s)	Animals ; Cats ; Coronavirus, Feline/immunology ; Coronavirus, Feline/isolation & purification ; Feline Infectious Peritonitis/blood ; Feline Infectious Peritonitis/diagnosis ; Immunohistochemistry/veterinary ; Prospective Studies ; Sensitivity and Specificity
Keywords	covid19
Language	English
Publishing date	2017-04
Publishing country	England
Document type	Evaluation Studies ; Journal Article
ZDB-ID	2049047-1
ISSN	1532-2750 ; 1098-612X
ISSN (online)	1532-2750
ISSN	1098-612X
DOI	10.1177/1098612X16630357
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Immunocytochemistry of mesenteric lymph node fine-needle aspirates in the diagnosis of feline infectious peritonitis

Felten, Sandra / Hartmann, Katrin / Doerfelt, Stefanie / Sangl, Laura / Hirschberger, Johannes / Matiasek, Kaspar

Journal of Veterinary Diagnostic Investigation. 2019 Mar., v. 31, no. 2 p.210-216

2019

Abstract	Immunohistochemistry (IHC) of tissue samples is considered the gold standard for diagnosing feline infectious peritonitis (FIP), and, in cats without body cavity effusion, IHC is the only method available to establish definitive antemortem diagnosis. However, IHC requires invasive tissue sample collection. We evaluated sensitivity and specificity of an immunocytochemical assay of fine-needle aspirates (FNAs) of mesenteric lymph nodes that can be obtained noninvasively by ultrasound-guided aspiration to diagnose FIP. FNAs of mesenteric lymph nodes were obtained postmortem from 41 cats suspected of having FIP based on clinical and/or laboratory findings. FIP was confirmed immunohistochemically in 30 cats. In the other 11 cats, a disease other than FIP, which explained the clinical signs, was diagnosed histopathologically. Immunocytochemistry (ICC) was performed as an avidin–biotin complex method using a monoclonal anti-FCoV IgG 2A. Sensitivity, specificity, negative and positive predictive values (NPV, PPV, respectively) including 95% confidence intervals (95% CIs) were determined. ICC was positive in 17 of 30 cats with FIP, but also in 1 of 11 control cats that was diagnosed with lymphoma. Sensitivity of ICC was 53% (95% CI: 34–72); specificity 91% (95% CI: 59–100); NPV 42% (95% CI: 22–63); and PPV 94% (95% CI: 71–100). In a lethal disease such as FIP, specificity is most important in order to avoid euthanasia of unaffected cats. Given that a false-positive result occurred and FIP was correctly detected in only approximately half of the cases of FIP, ICC of mesenteric lymph node FNA alone cannot reliably confirm or exclude FIP, but can be a helpful test in conjunction with other diagnostic measures.
Keywords	euthanasia ; false positive results ; feline infectious peritonitis ; histopathology ; immunocytochemistry ; immunohistochemistry ; lymph ; lymph nodes ; lymphoma ; ultrasonography ; fine-needle aspiration ; lymph node ; covid19
Language	English
Dates of publication	2019-03
Size	p. 210-216.
Publishing place	SAGE Publications
Document type	Article ; Online
Note	Resource is Open Access
ZDB-ID	287603-6
ISSN	1943-4936 ; 1040-6387
ISSN (online)	1943-4936
ISSN	1040-6387
DOI	10.1177/1040638718825280
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Cologne/Königswinter

Zs.A 3645: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Detection of feline coronavirus mutations in paraffin-embedded tissues in cats with feline infectious peritonitis and controls

Sangl, Laura / Matiasek, Kaspar / Felten, Sandra / Gründl, Stefanie / Bergmann, Michele / Balzer, Hans-Jörg / Pantchev, Nikola / Leutenegger, Christian M / Hartmann, Katrin

Journal of feline medicine and surgery. 2019 Feb., v. 21, no. 2

2019

Abstract: The amino acid substitutions M1058L and S1060A in the spike protein of feline coronavirus (FCoV) have been postulated to be responsible for the development of the pathogenic feline infectious peritonitis virus (FIPV), which causes feline infectious ... ...

Abstract	The amino acid substitutions M1058L and S1060A in the spike protein of feline coronavirus (FCoV) have been postulated to be responsible for the development of the pathogenic feline infectious peritonitis virus (FIPV), which causes feline infectious peritonitis (FIP). The aim of the following study was to investigate the presence of mutated virus in tissue samples of cats with and without FIP. The study population consisted of 64 cats, 34 of which were diagnosed with FIP and 30 control cats. All cases underwent autopsy, histopathology and immunohistochemistry (IHC) for FCoV. Furthermore, a genotype-discriminating quantitative reverse transcriptase PCR (RT-qPCR) was performed on shavings of paraffin-embedded tissues to discriminate between cats with FIP and controls, and the sensitivity and specificity of this discriminating RT-qPCR were calculated using 95% confidence intervals (CIs). Specificity of genotype-discriminating RT-qPCR was 100.0% (95% CI 88.4–100.0), and sensitivity was 70.6% (95% CI 52.5–84.9). In cats with FIP, 24/34 tested positive for FIPV. In samples of three control cats and in seven cats with FIP, FCoV was found, but genotyping was not possible owing to low FCoV RNA concentrations. Out of the positive samples, 23 showed the amino acid substitution M1058L in the spike protein and none the substitution S1060A. One sample in a cat with FIP revealed a mixed population of non-mutated FCoV and FIPV (mixed genotype). For one sample genotyping was not possible despite high viral load, and two samples were negative for FCoV. As none of the control animals showed FCoV amino acid substitutions previously demonstrated in cats with FIP, it can be presumed that the substitution M1058L correlates with the presence of FIP. FCoV was detected in low concentration in tissues of control animals, confirming the ability of FCoV to spread systemically. The fact that no negative controls were included in the IHC protocol could potentially lead to an underestimation of the sensitivity of the RT-qPCR.
Keywords	Feline coronavirus ; RNA ; amino acid substitution ; cats ; confidence interval ; feline infectious peritonitis ; genotype ; genotyping ; histopathology ; immunohistochemistry ; necropsy ; quantitative polymerase chain reaction ; reverse transcriptase polymerase chain reaction ; tissues ; viral load ; viruses ; covid19
Language	English
Dates of publication	2019-02
Size	p. 133-142.
Publishing place	SAGE Publications
Document type	Article
ZDB-ID	2049047-1
ISSN	1532-2750 ; 1098-612X
ISSN (online)	1532-2750
ISSN	1098-612X
DOI	10.1177/1098612X18762883
Database	NAL-Catalogue (AGRICOLA)

Order via subito

To top

Full text online

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito