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Article ; Online: Detection of porcine epidemic diarrhea virus-neutralizing antibody using high-throughput imaging cytometry.

Sarmento, Luciana V / Poonsuk, Korakrit / Tian, Liying / Mora-Díaz, Juan C / Main, Rodger G / Baum, David H / Zimmerman, Jeffrey J / Giménez-Lirola, Luis G

Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc

2020 Volume 32, Issue 2, Page(s) 324–328

Abstract: Porcine epidemic diarrhea virus (PEDV) is an emerging porcine coronavirus that causes a tremendous economic burden on the swine industry. The assessment of PEDV-neutralizing antibody levels provides a valuable tool to assess and predict herd immunity. We ...

Abstract	Porcine epidemic diarrhea virus (PEDV) is an emerging porcine coronavirus that causes a tremendous economic burden on the swine industry. The assessment of PEDV-neutralizing antibody levels provides a valuable tool to assess and predict herd immunity. We evaluated the performance of a PEDV imaging cytometry-based high-throughput neutralization test (HTNT) and compared the HTNT to a fluorescent focus neutralization (FFN) assay using serum samples from pigs of known PEDV infection status (
MeSH term(s)	Animals ; Antibodies, Neutralizing/blood ; Antibodies, Viral/blood ; Coronavirus Infections/diagnosis ; Coronavirus Infections/veterinary ; Coronavirus Infections/virology ; High-Throughput Screening Assays/methods ; High-Throughput Screening Assays/veterinary ; Image Cytometry/methods ; Image Cytometry/veterinary ; Neutralization Tests/methods ; Neutralization Tests/veterinary ; Porcine epidemic diarrhea virus/isolation & purification ; Swine ; Swine Diseases/diagnosis ; Swine Diseases/virology
Chemical Substances	Antibodies, Neutralizing ; Antibodies, Viral
Keywords	covid19
Language	English
Publishing date	2020-02-15
Publishing country	United States
Document type	Evaluation Study ; Journal Article
ZDB-ID	287603-6
ISSN	1943-4936 ; 1040-6387
ISSN (online)	1943-4936
ISSN	1040-6387
DOI	10.1177/1040638720903346
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Article: Detection of porcine epidemic diarrhea virus–neutralizing antibody using high-throughput imaging cytometry

Sarmento, Luciana V / Poonsuk, Korakrit / Tian, Liying / Mora-Díaz, Juan C / Main, Rodger G / Baum, David H / Zimmerman, Jeffrey J / Giménez-Lirola, Luis G

Journal of veterinary diagnostic investigation. 2020 Mar., v. 32, no. 2

2020

Abstract	Porcine epidemic diarrhea virus (PEDV) is an emerging porcine coronavirus that causes a tremendous economic burden on the swine industry. The assessment of PEDV-neutralizing antibody levels provides a valuable tool to assess and predict herd immunity. We evaluated the performance of a PEDV imaging cytometry–based high-throughput neutralization test (HTNT) and compared the HTNT to a fluorescent focus neutralization (FFN) assay using serum samples from pigs of known PEDV infection status (n = 159). Estimates of diagnostic sensitivity and specificity for HTNT and FFN assays derived from receiver-operator characteristic (ROC) curve analyses showed that both PEDV FFN and HTNT provided excellent diagnostic performance. However, in the laboratory, imaging cytometry provided an objective and semi-automated approach that removed human subjectivity from the testing process and reduced the read-time of a 96-well plate to < 4 min. In addition, imaging cytometry facilitated the rapid collection and long-term storage of test images and data for further evaluation or client consultation. For PEDV and other pathogens, imaging cytometry could provide distinct advantages over classic virus neutralization or FFN assays for the detection and quantitation of neutralizing antibody.
Keywords	Porcine epidemic diarrhea virus ; animal viruses ; blood serum ; diagnostic sensitivity ; diarrhea ; fluorescence ; herd immunity ; humans ; image analysis ; neutralization ; neutralization tests ; neutralizing antibodies ; pork industry ; storage time ; swine ; covid19
Language	English
Dates of publication	2020-03
Size	p. 324-328.
Publishing place	SAGE Publications
Document type	Article
ZDB-ID	287603-6
ISSN	1943-4936 ; 1040-6387
ISSN (online)	1943-4936
ISSN	1040-6387
DOI	10.1177/1040638720903346
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Impact of the immune profiles of hypertensive patients with and without obesity on COVID-19 severity.

Moll-Bernardes, Renata / Ferreira, Juliana R / Sousa, Andréa Silvestre / Tortelly, Mariana B / Pimentel, Adriana L / Figueiredo, Ana Cristina B S / Schaustz, Eduardo B / Secco, José Carlos Pizzolante / Sales, Allan Robson Kluser / Terzi, Flavia V O / Xavier de Brito, Adriana / Sarmento, Renée O / Noya-Rabelo, Marcia M / Fortier, Sergio / Matos E Silva, Flavia A / Vera, Narendra / Conde, Luciana / Cabral-Castro, Mauro Jorge / Albuquerque, Denilson C /

Rosado de-Castro, Paulo / Camargo, Gabriel C / Pinheiro, Martha V T / Souza, Olga F / Bozza, Fernando A / Luiz, Ronir R / Medei, Emiliano

International journal of obesity (2005)

2023 Volume 48, Issue 2, Page(s) 254–262

Abstract: Background: Comorbidities such as obesity, hypertension, and diabetes are associated with COVID-19 development and severity, probably due to immune dysregulation; however, the mechanisms underlying these associations are not clear. The immune signatures ...

Abstract	Background: Comorbidities such as obesity, hypertension, and diabetes are associated with COVID-19 development and severity, probably due to immune dysregulation; however, the mechanisms underlying these associations are not clear. The immune signatures of hypertensive patients with obesity with COVID-19 may provide new insight into the mechanisms of immune dysregulation and progression to severe disease in these patients. Methods: Hypertensive patients were selected prospectively from a multicenter registry of adults hospitalized with COVID-19 and stratified according to obesity (BMI ≥ 30 kg/m²). Clinical data including baseline characteristics, complications, treatment, and 46 immune markers were compared between groups. Logistic regression was performed to identify variables associated with the risk of COVID-19 progression in each group. Results: The sample comprised 213 patients (89 with and 124 without obesity). The clinical profiles of patients with and without obesity differed, suggesting potential interactions with COVID-19 severity. Relative to patients without obesity, patients with obesity were younger and fewer had cardiac disease and myocardial injury. Patients with obesity had higher EGF, GCSF, GMCSF, interleukin (IL)-1ra, IL-5, IL-7, IL-8, IL-15, IL-1β, MCP 1, and VEGF levels, total lymphocyte counts, and CD8 Conclusion: Our findings suggest that clinical and immune variables and obesity interact synergistically to increase the COVID-19 progression risk. The immune signatures of hypertensive patients with and without obesity severe COVID-19 highlight differences in immune dysregulation mechanisms, with potential therapeutic applications.
MeSH term(s)	Adult ; Humans ; CD8-Positive T-Lymphocytes ; COVID-19/complications ; COVID-19/metabolism ; Epidermal Growth Factor/metabolism ; Vascular Endothelial Growth Factor A ; HLA-DR Antigens/metabolism ; Hypertension/complications ; Hypertension/epidemiology ; Hypertension/metabolism ; Obesity/complications ; Obesity/metabolism ; Diabetes Mellitus
Chemical Substances	Epidermal Growth Factor (62229-50-9) ; Vascular Endothelial Growth Factor A ; HLA-DR Antigens
Language	English
Publishing date	2023-11-06
Publishing country	England
Document type	Multicenter Study ; Journal Article
ZDB-ID	752409-2
ISSN	1476-5497 ; 0307-0565
ISSN (online)	1476-5497
ISSN	0307-0565
DOI	10.1038/s41366-023-01407-0
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Zs.A 1325: Show issues

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Article: Detection of porcine epidemic diarrhea virus-neutralizing antibody using high-throughput imaging cytometry

Sarmento, Luciana V / Poonsuk, Korakrit / Tian, Liying / Mora-Díaz, Juan C / Main, Rodger G / Baum, David H / Zimmerman, Jeffrey J / Giménez-Lirola, Luis G

J Vet Diagn Invest

Abstract	Porcine epidemic diarrhea virus (PEDV) is an emerging porcine coronavirus that causes a tremendous economic burden on the swine industry. The assessment of PEDV-neutralizing antibody levels provides a valuable tool to assess and predict herd immunity. We evaluated the performance of a PEDV imaging cytometry-based high-throughput neutralization test (HTNT) and compared the HTNT to a fluorescent focus neutralization (FFN) assay using serum samples from pigs of known PEDV infection status (n = 159). Estimates of diagnostic sensitivity and specificity for HTNT and FFN assays derived from receiver-operator characteristic (ROC) curve analyses showed that both PEDV FFN and HTNT provided excellent diagnostic performance. However, in the laboratory, imaging cytometry provided an objective and semi-automated approach that removed human subjectivity from the testing process and reduced the read-time of a 96-well plate to < 4 min. In addition, imaging cytometry facilitated the rapid collection and long-term storage of test images and data for further evaluation or client consultation. For PEDV and other pathogens, imaging cytometry could provide distinct advantages over classic virus neutralization or FFN assays for the detection and quantitation of neutralizing antibody.
Keywords	covid19
Publisher	WHO
Document type	Article
Note	WHO #Covidence: #726122
Database	COVID19

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Article ; Online: Increased protein intake is associated with uncontrolled blood pressure by 24-hour ambulatory blood pressure monitoring in patients with type 2 diabetes.

Mattos, Carolina B / Viana, Luciana V / Paula, Tatiana P / Sarmento, Roberta A / Almeida, Jussara C / Gross, Jorge L / Azevedo, Mirela J

Journal of the American College of Nutrition

2015 Volume 34, Issue 3, Page(s) 232–239

Abstract: Objective: To analyze possible associations of dietary components, especially protein intake, with blood pressure (BP) during ambulatory BP monitoring (ABPM) in patients with type 2 diabetes.: Methods: In this cross-sectional study, BP of outpatients ...

Abstract	Objective: To analyze possible associations of dietary components, especially protein intake, with blood pressure (BP) during ambulatory BP monitoring (ABPM) in patients with type 2 diabetes. Methods: In this cross-sectional study, BP of outpatients with type 2 diabetes was evaluated by 24-hour ABPM (Spacelabs 90207) and usual diet by 3-day weighed diet records. Patients were divided into 2 groups according to their daytime ABPM: uncontrolled BP (systolic BP ≥ 135 mmHg or diastolic BP ≥ 85 mmHg) and controlled BP (systolic BP < 135 mmHg and diastolic BP < 85 mmHg). Logistic regression models unadjusted and adjusted for possible confounders (covariates) were used to analyze the association of protein and uncontrolled BP. Results: A total of 121 patients with type 2 diabetes aged 62.3 years, 54.5% of whom were women, were studied. The uncontrolled BP group had higher glycated hemoglobin (HbA1C) values (8.4 ± 2.0 vs 7.6 ± 1.3%; p = 0.04) and consumed more protein (20.0 ± 3.8 vs 18.2 ± 3.6% of energy; p = 0.01) and meat, (2.6 [1.45, 2.95] vs 2.0 [1.49, 2.90] g/kg weight; p = 0.04) than the controlled BP group. In a multivariate analysis, protein intake (% of energy) increased the chance for uncontrolled BP (odds ratio [OR] = 1.16; 95% confidence interval [CI], 1.02, 1.30; p = 0.02), adjusted for body mass index (BMI), HbA1C, low-density lipoprotein (LDL) cholesterol, number of antihypertensive medications, and ethnicity. Meat consumption higher than 3.08 g/kg weight/day more than doubled the chance for uncontrolled BP (OR = 2.53; 95% CI, 1.01, 7.60; p = 0.03). Conclusion: High protein intake and meat consumption were associated with high daytime ABPM values in patients with type 2 diabetes. Reducing meat intake might represent an additional dietary intervention in hypertensive patients with type 2 diabetes.
MeSH term(s)	Aged ; Blood Pressure Monitoring, Ambulatory ; Cross-Sectional Studies ; Diabetes Mellitus, Type 2/complications ; Diabetes Mellitus, Type 2/diet therapy ; Diabetes Mellitus, Type 2/physiopathology ; Diabetic Angiopathies/etiology ; Dietary Proteins/adverse effects ; Energy Intake ; Female ; Glycated Hemoglobin A/analysis ; Humans ; Hypertension/etiology ; Logistic Models ; Male ; Meat ; Middle Aged
Chemical Substances	Dietary Proteins ; Glycated Hemoglobin A
Language	English
Publishing date	2015
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	603204-7
ISSN	1541-1087 ; 0731-5724
ISSN (online)	1541-1087
ISSN	0731-5724
DOI	10.1080/07315724.2014.926155
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Zs.A 1759: Show issues

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Article ; Online: Relationship between Mitral Regurgitation and Transcatheter Aortic Valve Implantation: a Multi-Institutional Follow-up Study.

Cunha, Luciana de Cerjat Bernardes P da / Guerios, Enio Eduardo / Cunha, Claudio Leinig Pereira da / Carvalho, Luiz A / Lemos Neto, Pedro / Sarmento-Leite, Rogério / Abizaid, Alexandre A / Mangione, José Antonio / Oliveira, Adriano Dourado / Siciliano, Alexandre / Esteves, Vinicius / Brito, Fábio Sândoli de

Arquivos brasileiros de cardiologia

2021 Volume 116, Issue 6, Page(s) 1059–1069

Abstract: Background: Mitral regurgitation (MR) is prevalent in patients undergoing transcatheter aortic valve implantation (TAVI). There are some controversies about the prognostic impact of MR in survival of TAVI patients.: Objective: To examine the ... ...

Title translation	A Relação entre Regurgitação Mitral e Implante Transcateter de Válvula Aórtica: um Estudo de Acompanhamento Multi-Institucional.
Abstract	Background: Mitral regurgitation (MR) is prevalent in patients undergoing transcatheter aortic valve implantation (TAVI). There are some controversies about the prognostic impact of MR in survival of TAVI patients. Objective: To examine the relationship between TAVI and MR in a patient population from the Brazilian TAVI Registry. Methods: Seven hundred and ninety-five patients from the Brazilian TAVI Registry were divided at baseline, discharge, and follow-up according to their MR grade as follows: absent/mild (AMMR) or moderate/severe (MSMR). They were subsequently regrouped according to their immediate and late changes in MR severity after TAVI as follows: no change, improved, or worsened MR. Predictors and prognostic impact on baseline as well as changes in MR severity were analyzed. Statistical significance was set at p < 0.05. Results: Baseline MSMR was present in 19.3% of patients and was a predictor of increased late mortality. Immediately after TAVI, 47.4 % of cases improved to AMMR, predicted by a higher Society of Thoracic Surgeons score and a higher grade of baseline aortic regurgitation. Upon follow-up, 9.2% of cases of AMMR worsened to MSMR, whereas 36.8% of cases of MSMR improved to AMMR. Lower baseline left ventricular ejection fraction (LVEF) and improvement in LVEF at follow-up were predictors of MR improvement. Progressive worsening of MR upon follow-up was an independent predictor of higher late mortality after TAVI (p = 0.005). Conclusions: Baseline MSMR predicts late mortality after TAVI. Lower LVEF and improved LVEF at follow-up predict MR improvement after TAVI. Progressive worsening of MR severity at follow-up is an independent predictor of late mortality, which is a rare finding in the literature.
MeSH term(s)	Aortic Valve/surgery ; Aortic Valve Stenosis/surgery ; Brazil ; Follow-Up Studies ; Heart Valve Prosthesis Implantation ; Humans ; Mitral Valve Insufficiency/surgery ; Severity of Illness Index ; Stroke Volume ; Transcatheter Aortic Valve Replacement ; Treatment Outcome ; Ventricular Function, Left
Language	Portuguese
Publishing date	2021-06-14
Publishing country	Brazil
Document type	Journal Article
ZDB-ID	730261-7
ISSN	1678-4170 ; 0066-782X
ISSN (online)	1678-4170
ISSN	0066-782X
DOI	10.36660/abc.20190772
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Zs.A 548: Show issues

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Article: Increased Protein Intake Is Associated With Uncontrolled Blood Pressure by 24-Hour Ambulatory Blood Pressure Monitoring in Patients With Type 2 Diabetes

Mattos, Carolina B / Jorge L. Gross / Jussara C. Almeida / Luciana V. Viana / Mirela J. Azevedo / Roberta A. Sarmento / Tatiana P. Paula

Journal of the American College of Nutrition. 2015 May 4, v. 34, no. 3

2015

Abstract: Objective: To analyze possible associations of dietary components, especially protein intake, with blood pressure (BP) during ambulatory BP monitoring (ABPM) in patients with type 2 diabetes. Methods: In this cross-sectional study, BP of outpatients with ...

Abstract	Objective: To analyze possible associations of dietary components, especially protein intake, with blood pressure (BP) during ambulatory BP monitoring (ABPM) in patients with type 2 diabetes. Methods: In this cross-sectional study, BP of outpatients with type 2 diabetes was evaluated by 24-hour ABPM (Spacelabs 90207) and usual diet by 3-day weighed diet records. Patients were divided into 2 groups according to their daytime ABPM: uncontrolled BP (systolic BP â¥ 135Â mmHg or diastolic BP â¥ 85Â mmHg) and controlled BP (systolic BP < 135Â mmHg and diastolic BP < 85Â mmHg). Logistic regression models unadjusted and adjusted for possible confounders (covariates) were used to analyze the association of protein and uncontrolled BP. Results: A total of 121 patients with type 2 diabetes aged 62.3Â years, 54.5% of whom were women, were studied. The uncontrolled BP group had higher glycated hemoglobin (HbA1C) values (8.4 Â± 2.0Â vs 7.6 Â± 1.3%; p = 0.04) and consumed more protein (20.0 Â± 3.8Â vs 18.2 Â± 3.6% of energy; p = 0.01) and meat, (2.6 [1.45, 2.95] vs 2.0 [1.49, 2.90] g/kg weight; p = 0.04) than the controlled BP group. In a multivariate analysis, protein intake (% of energy) increased the chance for uncontrolled BP (odds ratio [OR] = 1.16; 95% confidence interval [CI], 1.02, 1.30; p = 0.02), adjusted for body mass index (BMI), HbA1C, low-density lipoprotein (LDL) cholesterol, number of antihypertensive medications, and ethnicity. Meat consumption higher than 3.08Â g/kg weight/day more than doubled the chance for uncontrolled BP (OR = 2.53; 95% CI, 1.01, 7.60; p = 0.03). Conclusion: High protein intake and meat consumption were associated with high daytime ABPM values in patients with type 2 diabetes. Reducing meat intake might represent an additional dietary intervention in hypertensive patients with type 2 diabetes.
Keywords	blood pressure ; body mass index ; confidence interval ; cross-sectional studies ; energy ; glycohemoglobin ; low density lipoprotein cholesterol ; meat ; meat consumption ; monitoring ; multivariate analysis ; nationalities and ethnic groups ; noninsulin-dependent diabetes mellitus ; nutritional intervention ; odds ratio ; patients ; protein intake ; regression analysis ; women
Language	English
Dates of publication	2015-0504
Size	p. 232-239.
Publishing place	Routledge
Document type	Article
ZDB-ID	603204-7
ISSN	1541-1087 ; 0731-5724
ISSN (online)	1541-1087
ISSN	0731-5724
DOI	10.1080/07315724.2014.926155
Database	NAL-Catalogue (AGRICOLA)

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Article: Pathophysiology and mechanisms of hearing impairment related to neonatal infection diseases.

Capra, Daniela / DosSantos, Marcos F / Sanz, Carolina K / Acosta Filha, Lionete Gall / Nunes, Priscila / Heringer, Manoela / Ximenes-da-Silva, Adriana / Pessoa, Luciana / de Mattos Coelho-Aguiar, Juliana / da Fonseca, Anna Carolina Carvalho / Mendes, Carmelita Bastos / da Rocha, Lanni Sarmento / Devalle, Sylvie / Niemeyer Soares Filho, Paulo / Moura-Neto, Vivaldo

Frontiers in microbiology

2023 Volume 14, Page(s) 1162554

Abstract: The inner ear, the organ of equilibrium and hearing, has an extraordinarily complex and intricate arrangement. It contains highly specialized structures meticulously tailored to permit auditory processing. However, hearing also relies on both peripheral ... ...

Abstract	The inner ear, the organ of equilibrium and hearing, has an extraordinarily complex and intricate arrangement. It contains highly specialized structures meticulously tailored to permit auditory processing. However, hearing also relies on both peripheral and central pathways responsible for the neuronal transmission of auditory information from the cochlea to the corresponding cortical regions. Understanding the anatomy and physiology of all components forming the auditory system is key to better comprehending the pathophysiology of each disease that causes hearing impairment. In this narrative review, the authors focus on the pathophysiology as well as on cellular and molecular mechanisms that lead to hearing loss in different neonatal infectious diseases. To accomplish this objective, the morphology and function of the main structures responsible for auditory processing and the immune response leading to hearing loss were explored. Altogether, this information permits the proper understanding of each infectious disease discussed.
Language	English
Publishing date	2023-04-14
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2587354-4
ISSN	1664-302X
ISSN	1664-302X
DOI	10.3389/fmicb.2023.1162554
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Article ; Online: Domestic pigs have low susceptibility to H5N1 highly pathogenic avian influenza viruses.

Lipatov, Aleksandr S / Kwon, Yong Kuk / Sarmento, Luciana V / Lager, Kelly M / Spackman, Erica / Suarez, David L / Swayne, David E

PLoS pathogens

2008 Volume 4, Issue 7, Page(s) e1000102

Abstract: Genetic reassortment of H5N1 highly pathogenic avian influenza viruses (HPAI) with currently circulating human influenza A strains is one possibility that could lead to efficient human-to-human transmissibility. Domestic pigs which are susceptible to ... ...

Abstract	Genetic reassortment of H5N1 highly pathogenic avian influenza viruses (HPAI) with currently circulating human influenza A strains is one possibility that could lead to efficient human-to-human transmissibility. Domestic pigs which are susceptible to infection with both human and avian influenza A viruses are one of the natural hosts where such reassortment events could occur. Virological, histological and serological features of H5N1 virus infection in pigs were characterized in this study. Two- to three-week-old domestic piglets were intranasally inoculated with 10(6) EID(50) of A/Vietnam/1203/04 (VN/04), A/chicken/Indonesia/7/03 (Ck/Indo/03), A/Whooper swan/Mongolia/244/05 (WS/Mong/05), and A/Muscovy duck/Vietnam/ 209/05 (MDk/VN/05) viruses. Swine H3N2 and H1N1 viruses were studied as a positive control for swine influenza virus infection. The pathogenicity of the H5N1 HPAI viruses was also characterized in mouse and ferret animal models. Intranasal inoculation of pigs with H5N1 viruses or consumption of infected chicken meat did not result in severe disease. Mild weight loss was seen in pigs inoculated with WS/Mong/05, Ck/Indo/03 H5N1 and H1N1 swine influenza viruses. WS/Mong/05, Ck/Indo/03 and VN/04 viruses were detected in nasal swabs of inoculated pigs mainly on days 1 and 3. Titers of H5N1 viruses in nasal swabs were remarkably lower compared with those of swine influenza viruses. Replication of all four H5N1 viruses in pigs was restricted to the respiratory tract, mainly to the lungs. Titers of H5N1 viruses in the lungs were lower than those of swine viruses. WS/Mong/05 virus was isolated from trachea and tonsils, and MDk/VN/05 virus was isolated from nasal turbinate of infected pigs. Histological examination revealed mild to moderate bronchiolitis and multifocal alveolitis in the lungs of pigs infected with H5N1 viruses, while infection with swine influenza viruses resulted in severe tracheobronchitis and bronchointerstitial pneumonia. Pigs had low susceptibility to infection with H5N1 HPAI viruses. Inoculation of pigs with H5N1 viruses resulted in asymptomatic to mild symptomatic infection restricted to the respiratory tract and tonsils in contrast to mouse and ferrets animal models, where some of the viruses studied were highly pathogenic and replicated systemically.
MeSH term(s)	Amino Acid Sequence ; Animals ; Animals, Newborn ; Disease Models, Animal ; Disease Susceptibility/veterinary ; Disease Susceptibility/virology ; Ferrets ; Hemagglutinins, Viral/chemistry ; Hemagglutinins, Viral/genetics ; Influenza A Virus, H5N1 Subtype/pathogenicity ; Influenza A Virus, H5N1 Subtype/physiology ; Lung/pathology ; Lung/virology ; Mice ; Orthomyxoviridae Infections/immunology ; Orthomyxoviridae Infections/pathology ; Orthomyxoviridae Infections/veterinary ; RNA, Viral/analysis ; Sequence Analysis, DNA ; Species Specificity ; Swine ; Swine Diseases/immunology ; Swine Diseases/pathology ; Swine Diseases/virology ; Virus Replication
Chemical Substances	Hemagglutinins, Viral ; RNA, Viral
Language	English
Publishing date	2008-07-11
Publishing country	United States
Document type	Comparative Study ; Journal Article ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	2205412-1
ISSN	1553-7374 ; 1553-7366
ISSN (online)	1553-7374
ISSN	1553-7366
DOI	10.1371/journal.ppat.1000102
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Article ; Online: Effects of seasonality, trophic state and landscape properties on CO

Junger, Pedro Ciarlini / Dantas, Fabíola da Costa Catombé / Nobre, Regina Lucia Guimarães / Kosten, Sarian / Venticinque, Eduardo Martins / Araújo, Fernando de Carvalho / Sarmento, Hugo / Angelini, Ronaldo / Terra, Iagê / Gaudêncio, Andrievisk / They, Ng Haig / Becker, Vanessa / Cabral, Camila Rodrigues / Quesado, Letícia / Carneiro, Luciana Silva / Caliman, Adriano / Amado, André Megali

The Science of the total environment

2019 Volume 664, Page(s) 283–295

Abstract: The role of tropical lakes and reservoirs in the global carbon cycle has received increasing attention in the past decade, but our understanding of its variability is still limited. The metabolism of tropical systems may differ profoundly from temperate ... ...

Abstract	The role of tropical lakes and reservoirs in the global carbon cycle has received increasing attention in the past decade, but our understanding of its variability is still limited. The metabolism of tropical systems may differ profoundly from temperate systems due to the higher temperatures and wider variations in precipitation. Here, we investigated the spatial and temporal patterns of the variability in the partial pressure of carbon dioxide (pCO
Language	English
Publishing date	2019-01-24
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	121506-1
ISSN	1879-1026 ; 0048-9697
ISSN (online)	1879-1026
ISSN	0048-9697
DOI	10.1016/j.scitotenv.2019.01.273
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