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Book ; Thesis: Sekretion und Endocytose von Neurotrophinen

Krüttgen, Alexander

Implikationen für Neurobiologie und Neuropathologie

2006

Author's details	vorgelegt von Alexander Krüttgen
Language	German
Size	Getr. Zählung : Ill., graph. Darst.
Publishing country	Germany
Document type	Book ; Thesis
Thesis / German Habilitation thesis	Aachen, Techn. Hochsch., Habil.-Schr., 2006
HBZ-ID	HT014813242
Database	Catalogue ZB MED Medicine, Health

In stock of ZB MED Cologne/Königswinter

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2008 E 463	order for loan	Magazin

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Article ; Online: Reply to the letter from Dr. Nikolayevskyy et al. referring to our publication published in the Journal of Virological Methods 298 (2021)11:4295 "Evaluation of the QuantiFERON SARS-CoV-2 Interferon-ɣ release assay in mRNA-1273 vaccinated health care workers".

Krüttgen, Alexander / Kleines, Michael

Journal of virological methods

2022 Volume 302, Page(s) 114473

Language	English
Publishing date	2022-01-21
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	8013-5
ISSN	1879-0984 ; 0166-0934
ISSN (online)	1879-0984
ISSN	0166-0934
DOI	10.1016/j.jviromet.2022.114473
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Humoral immune response to SARS-CoV-2 mRNA vaccines is associated with choice of vaccine and systemic adverse reactions.

Klingel, Hanna / Krüttgen, Alexander / Imöhl, Matthias / Kleines, Michael

Clinical and experimental vaccine research

2023 Volume 12, Issue 1, Page(s) 60–69

Abstract: Purpose: Although the fast development of safe and effective messenger RNA (mRNA) vaccines against severe acute respiratory syndrome coronavirus 2 has been a success, waning humoral immunity has led to the recommendation of booster immunization. However, ...

Abstract	Purpose: Although the fast development of safe and effective messenger RNA (mRNA) vaccines against severe acute respiratory syndrome coronavirus 2 has been a success, waning humoral immunity has led to the recommendation of booster immunization. However, knowledge of the humoral immune response to different booster strategies and the association with adverse reactions is limited. Materials and methods: We investigated adverse reactions and anti-spike protein immunoglobulin G (IgG) concentrations among health care workers who received primary immunization with mRNA-1273 and booster immunization with mRNA-1273 or BNT162b2. Results: Adverse reactions were reported by 85.1% after the first dose, 94.7% after the second dose, 87.5% after a third dose of BNT162b2, and 86.0% after a third dose of mRNA-1273. They lasted for a median of 1.8, 2.0, 2.5, and 1.8 days, respectively; 6.4%, 43.6%, and 21.0% of the participants were unable to work after the first, second, and third vaccination, respectively, which should be considered when scheduling vaccinations among essential workers. Booster immunization induced a 13.75-fold (interquartile range, 9.30-24.47) increase of anti-spike protein IgG concentrations with significantly higher concentrations after homologous compared to heterologous vaccination. We found an association between fever, chills, and arthralgia after the second vaccination and anti-spike protein IgG concentrations indicating a linkage between adverse reactions, inflammation, and humoral immune response. Conclusion: Further investigations should focus on the possible advantages of homologous and heterologous booster vaccinations and their capability of stimulating memory B-cells. Additionally, understanding inflammatory processes induced by mRNA vaccines might help to improve reactogenicity while maintaining immunogenicity and efficacy.
Language	English
Publishing date	2023-01-31
Publishing country	Korea (South)
Document type	Journal Article
ZDB-ID	2684652-4
ISSN	2287-366X ; 2287-3651
ISSN (online)	2287-366X
ISSN	2287-3651
DOI	10.7774/cevr.2023.12.1.60
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Book ; Thesis: Struktur-Funktions-Analyse von humanem Interleukin-6

Krüttgen, Alexander

1994

Author's details	vorgelegt von Alexander Krüttgen
Language	German
Size	IX, 45 S. : Ill., graph. Darst.
Publishing country	Germany
Document type	Book ; Thesis
Thesis / German Habilitation thesis	Aachen, Techn. Hochsch., Diss., 1994
HBZ-ID	HT006564287
Database	Catalogue ZB MED Medicine, Health

In stock of ZB MED Cologne/Königswinter

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95 D 3242	order for loan	Magazin

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Article: Severity of adverse reactions is associated with T-cell response in mRNA-1273 vaccinated health care workers.

Klingel, Hanna / Lauen, Maike / Krüttgen, Alexander / Imöhl, Matthias / Kleines, Michael

Clinical and experimental vaccine research

2022 Volume 11, Issue 1, Page(s) 121–124

Abstract: Knowledge about mRNA-1273 elicited T-cell response is limited. We investigated adverse reactions and interferon gamma release by specific T-cells among mRNA-1273 vaccinated health care workers. Seven to 13 weeks after complete vaccination low levels of ... ...

Abstract	Knowledge about mRNA-1273 elicited T-cell response is limited. We investigated adverse reactions and interferon gamma release by specific T-cells among mRNA-1273 vaccinated health care workers. Seven to 13 weeks after complete vaccination low levels of specific T-cells were detected not correlating with antibody response. Severity of symptoms after first and number of symptoms after second immunization were associated with T-cell response. Assessment of T-cell response in addition to antibody response is crucial because even few specific T-cells could add to protection against infection. Investigation of mRNA-1273 induced inflammatory processes might help improve reactogenicity and immunogenicity.
Language	English
Publishing date	2022-01-31
Publishing country	Korea (South)
Document type	Journal Article
ZDB-ID	2684652-4
ISSN	2287-366X ; 2287-3651
ISSN (online)	2287-366X
ISSN	2287-3651
DOI	10.7774/cevr.2022.11.1.121
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Large inter-individual variability of cellular and humoral immunological responses to mRNA-1273 (Moderna) vaccination against SARS-CoV-2 in health care workers.

Krüttgen, Alexander / Haase, Gerhard / Haefner, Helga / Imöhl, Matthias / Kleines, Michael

Clinical and experimental vaccine research

2022 Volume 11, Issue 1, Page(s) 96–103

Abstract: Purpose: Studies on the immune responses to severe acute respiratory syndrome coronavirus 2 vaccines are necessary to evaluate the ongoing vaccination programs by correlating serological response data and clinical effectiveness data. We performed a ... ...

Abstract	Purpose: Studies on the immune responses to severe acute respiratory syndrome coronavirus 2 vaccines are necessary to evaluate the ongoing vaccination programs by correlating serological response data and clinical effectiveness data. We performed a longitudinal immunological profiling of health care workers vaccinated with mRNA-1273 (Moderna, Cambridge, MA, USA). Half of these vaccinees had experienced a mild coronavirus disease 2019 (COVID-19) infection in the spring of 2020 ("COVID-recovered" cohort), whereas the other half of the vaccinees had no previous COVID-19 infection ("COVID-naive" cohort). Materials and methods: Serum was drawn at multiple time points and subjected to assays measuring anti-Spike immunoglobulin G (IgG), avidity of anti-Spike IgG, avidity of anti-receptor binding domain (RBD) IgG, virus neutralizing activity, and interferon-γ release from stimulated lymphocytes. Results: Between both cohorts and within each cohort, we found remarkable inter-individual differences regarding cellular and humoral immune responses to the Moderna mRNA-1273 vaccine. Conclusion: First, our study indicates that the success of mRNA-1273 vaccinations should be verified by serological assays in order to identify "low-responders" to vaccination. Second, the kinetics of anti-S IgG and neutralizing activity correlate well with clinical effectiveness data, thus explaining incipient protection against infection 2 weeks after the first dose of mRNA-1273 in COVID-naive vaccinees. Third, our IgG-avidity data indicate that this incipient protection is mediated by low-avidity anti-RBD IgG and low-avidity anti-S IgG.
Language	English
Publishing date	2022-01-31
Publishing country	Korea (South)
Document type	Journal Article
ZDB-ID	2684652-4
ISSN	2287-366X ; 2287-3651
ISSN (online)	2287-366X
ISSN	2287-3651
DOI	10.7774/cevr.2022.11.1.96
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Two novel SARS-CoV-2 surrogate virus neutralization assays are suitable for assessing successful immunization with mRNA-1273

Krüttgen, Alexander / Lauen, Maike / Klingel, Hanna / Imöhl, Matthias / Kleines, Michael

Journal of virological methods. 2022 Jan., v. 299

2022

Abstract: Due to large vaccination efforts with novel vaccines there is an increasing need for laboratory tests assessing successful immunizations with SARS-CoV-2 vaccines. Unfortunately classical neutralization assays are laborious, time-consuming and require an ... ...

Abstract	Due to large vaccination efforts with novel vaccines there is an increasing need for laboratory tests assessing successful immunizations with SARS-CoV-2 vaccines. Unfortunately classical neutralization assays are laborious, time-consuming and require an adequate biosafety level laboratory. Recently, convenient ELISA-based surrogate neutralization assays (sVNTs) for determination of neutralizing SARS-CoV-2 antibodies have been developed.Our study compares the two novel ELISA-based SARS-CoV-2 surrogate neutralization assays “cPass SARS-CoV-2 Surrogate Virus Neutralization Test Kit” (GenScript Biotech, USA) and the “TECO SARS-CoV-2 Neutralization Antibody Assay” (TECOmedical, Switzerland) using 93 sera drawn from health care workers (HCVs) 2–3 weeks following the second vaccination with mRNA-1273 and 40 control sera from the pre-SARS-CoV-2 era before 2019.We found a sensitivity of 100% and 91,4% and a specificity of 100% and 100% for the GenScript assay and the TECO assay, respectively. Both sVNTs show a high correlation with anti-S IgG. Moreover, both sVNTs correlate well with each other.Surrogate neutralization assays based on the RBD as bait feature a high specificity and sensitivity for identifying humoral neutralizing activity in individuals vaccinated with the spike-based vaccine mRNA-1273. Although these assays appear well-suited for confirming successful vaccinations with spike-based vaccines, additional studies should compare both assays regarding other purposes such as screening COVID-recovered patients or individuals vaccinated with inactivated whole virus vaccines.
Keywords	Severe acute respiratory syndrome coronavirus 2 ; analytical kits ; antibodies ; biosafety ; enzyme-linked immunosorbent assay ; health services ; neutralization ; neutralization tests ; vaccination ; vaccines ; viruses ; Switzerland
Language	English
Dates of publication	2022-01
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	8013-5
ISSN	1879-0984 ; 0166-0934
ISSN (online)	1879-0984
ISSN	0166-0934
DOI	10.1016/j.jviromet.2021.114297
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Cologne/Königswinter

Zs.A 1565: Show issues

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Article ; Online: Two novel SARS-CoV-2 surrogate virus neutralization assays are suitable for assessing successful immunization with mRNA-1273.

Krüttgen, Alexander / Lauen, Maike / Klingel, Hanna / Imöhl, Matthias / Kleines, Michael

Journal of virological methods

2021 Volume 299, Page(s) 114297

Abstract: Background: Due to large vaccination efforts with novel vaccines there is an increasing need for laboratory tests assessing successful immunizations with SARS-CoV-2 vaccines. Unfortunately classical neutralization assays are laborious, time-consuming ... ...

Abstract	Background: Due to large vaccination efforts with novel vaccines there is an increasing need for laboratory tests assessing successful immunizations with SARS-CoV-2 vaccines. Unfortunately classical neutralization assays are laborious, time-consuming and require an adequate biosafety level laboratory. Recently, convenient ELISA-based surrogate neutralization assays (sVNTs) for determination of neutralizing SARS-CoV-2 antibodies have been developed. Study design: Our study compares the two novel ELISA-based SARS-CoV-2 surrogate neutralization assays "cPass SARS-CoV-2 Surrogate Virus Neutralization Test Kit" (GenScript Biotech, USA) and the "TECO SARS-CoV-2 Neutralization Antibody Assay" (TECOmedical, Switzerland) using 93 sera drawn from health care workers (HCVs) 2-3 weeks following the second vaccination with mRNA-1273 and 40 control sera from the pre-SARS-CoV-2 era before 2019. Results: We found a sensitivity of 100% and 91,4% and a specificity of 100% and 100% for the GenScript assay and the TECO assay, respectively. Both sVNTs show a high correlation with anti-S IgG. Moreover, both sVNTs correlate well with each other. Conclusions: Surrogate neutralization assays based on the RBD as bait feature a high specificity and sensitivity for identifying humoral neutralizing activity in individuals vaccinated with the spike-based vaccine mRNA-1273. Although these assays appear well-suited for confirming successful vaccinations with spike-based vaccines, additional studies should compare both assays regarding other purposes such as screening COVID-recovered patients or individuals vaccinated with inactivated whole virus vaccines.
MeSH term(s)	Antibodies, Neutralizing ; Antibodies, Viral ; COVID-19 ; COVID-19 Vaccines ; Humans ; Immunization ; Neutralization Tests ; SARS-CoV-2 ; Spike Glycoprotein, Coronavirus ; Vaccination
Chemical Substances	Antibodies, Neutralizing ; Antibodies, Viral ; COVID-19 Vaccines ; Spike Glycoprotein, Coronavirus
Language	English
Publishing date	2021-09-23
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	8013-5
ISSN	1879-0984 ; 0166-0934
ISSN (online)	1879-0984
ISSN	0166-0934
DOI	10.1016/j.jviromet.2021.114297
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1565: Show issues

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Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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Jg. 1995 - 2021: Lesesall (1.OG)
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Article: Comparison of the SARS-CoV-2 Rapid antigen test to the real star Sars-CoV-2 RT PCR kit

Krüttgen, Alexander / Cornelissen, Christian G. / Dreher, Michael / Hornef, Mathias W. / Imöhl, Matthias / Kleines, Michael

Journal of virological methods. 2021 Feb., v. 288

2021

Abstract: There is an ongoing need for reliable antigen assays for timely and easy detection of individuals with acute SARS-CoV-2 infection. Using 75 swabs from patients previously tested positive by SARS-CoV-2 PCR and 75 swabs from patients previously tested ... ...

Abstract	There is an ongoing need for reliable antigen assays for timely and easy detection of individuals with acute SARS-CoV-2 infection. Using 75 swabs from patients previously tested positive by SARS-CoV-2 PCR and 75 swabs from patients previously tested negative by SARS-CoV-2 PCR, we investigated the sensitivity and specificity of the SARS-CoV-2 Rapid Antigen Test (Roche). We determined a specificity of 96 %. The assay’s sensitivity with samples with a cycle threshold of < 25, 25 - <30, 30 - <35, and> = 35 was 100 %, 95 %, 44.8 % and 22.2 %, respectively. We conclude that sensitivity and specificity of the antigen assay is inferior to the PCR assay. However, the antigen assay may be a quick and easy to perform alternative for differentiation of individuals contagious for SARS-CoV-2 from non-contagious individuals.
Keywords	Severe acute respiratory syndrome coronavirus 2 ; antigen detection ; antigens ; journals ; patients ; rapid methods
Language	English
Dates of publication	2021-02
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	8013-5
ISSN	1879-0984 ; 0166-0934
ISSN (online)	1879-0984
ISSN	0166-0934
DOI	10.1016/j.jviromet.2020.114024
Database	NAL-Catalogue (AGRICOLA)

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Article: Determination of SARS-CoV-2 antibodies with assays from Diasorin, Roche and IDvet

Krüttgen, Alexander / Cornelissen, Christian G. / Dreher, Michael / Hornef, Mathias W. / Imöhl, Matthias / Kleines, Michael

Journal of virological methods. 2021 Jan., v. 287

2021

Abstract: There is an ongoing need for highly reliable serological assays to detect individuals with past SARS-CoV-2 infection. Using 75 sera from patients tested positive or negative by SARS-CoV-2 PCR, we investigated the sensitivity and specificity of the ... ...

Abstract	There is an ongoing need for highly reliable serological assays to detect individuals with past SARS-CoV-2 infection. Using 75 sera from patients tested positive or negative by SARS-CoV-2 PCR, we investigated the sensitivity and specificity of the Liaison SARS-CoV-2 S1/S2 IgG assay (DiaSorin), the Elecsys Anti-SARS-CoV-2 assay (Roche), and the ID Screen SARS-CoV-2-N IgG indirect kit (IDVet). We determined a sensitivity of 95.5 %, 95.5 %, and 100 % and a specificity of 90.5 %, 96.2 %, and 92.5 % for the DiaSorin assay, the Roche assay, and the IDVet assay, respectively. We conclude that serologic assays combining very high sensitivity and specificity are still not commercially available for SARS-CoV-2. For maximizing sensitivity and specificity of SARS-CoV-2 serological diagnostics, the combination of two assays may be helpful.
Keywords	Severe acute respiratory syndrome coronavirus 2 ; antibodies ; blood serum ; diagnostic techniques ; immunologic techniques ; journals ; patients ; screening ; testing
Language	English
Dates of publication	2021-01
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	8013-5
ISSN	1879-0984 ; 0166-0934
ISSN (online)	1879-0984
ISSN	0166-0934
DOI	10.1016/j.jviromet.2020.113978
Database	NAL-Catalogue (AGRICOLA)

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