Article: Structural insight into dimeric interaction of the SARAH domains from Mst1 and RASSF family proteins in the apoptosis pathway.
Proceedings of the National Academy of Sciences of the United States of America
2007 Volume 104, Issue 22, Page(s) 9236–9241
Abstract: ... controlled by the interaction of SARAH (for Salvador/Rassf/Hippo) domains in the C-terminal part ... of tumor suppressor proteins. The Mst1 SARAH domain interacts with its homologous domain of Rassf1 and Rassf5 (also known ... structure of the human Mst1 SARAH domain and its heterotypic interaction with the Rassf5 and Salvador (Sav ...
Abstract | In eukaryotic cells, apoptosis and cell cycle arrest by the Ras --> RASSF --> MST pathway are controlled by the interaction of SARAH (for Salvador/Rassf/Hippo) domains in the C-terminal part of tumor suppressor proteins. The Mst1 SARAH domain interacts with its homologous domain of Rassf1 and Rassf5 (also known as Nore1) by forming a heterodimer that mediates the apoptosis process. Here, we describe the homodimeric structure of the human Mst1 SARAH domain and its heterotypic interaction with the Rassf5 and Salvador (Sav) SARAH domain. The Mst1 SARAH structure forms a homodimer containing two helices per monomer. An antiparallel arrangement of the long alpha-helices (h2/h2') provides an elongated binding interface between the two monomers, and the short 3(10) helices (h1/h1') are folded toward that of the other monomer. Chemical shift perturbation experiments identified an elongated, tight-binding interface with the Rassf5 SARAH domain and a 1:1 heterodimer formation. The linker region between the kinase and the SARAH domain is shown to be disordered in the free protein. These results imply a novel mode of interaction with RASSF family proteins and provide insight into the mechanism of apoptosis control by the SARAH domain. |
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MeSH term(s) | Amino Acid Sequence ; Apoptosis ; Cell Cycle Proteins/chemistry ; Cell Cycle Proteins/genetics ; Cell Cycle Proteins/metabolism ; Dimerization ; Humans ; Models, Molecular ; Molecular Sequence Data ; Nuclear Magnetic Resonance, Biomolecular ; Protein Binding ; Protein Folding ; Protein Structure, Secondary ; Protein Structure, Tertiary ; Protein-Serine-Threonine Kinases/chemistry ; Protein-Serine-Threonine Kinases/classification ; Protein-Serine-Threonine Kinases/genetics ; Protein-Serine-Threonine Kinases/metabolism ; Tumor Suppressor Proteins/chemistry ; Tumor Suppressor Proteins/classification ; Tumor Suppressor Proteins/genetics ; Tumor Suppressor Proteins/metabolism |
Chemical Substances | Cell Cycle Proteins ; SAV1 protein, human ; Tumor Suppressor Proteins ; Protein-Serine-Threonine Kinases (EC 2.7.11.1) |
Language | English |
Publishing date | 2007-05-29 |
Publishing country | United States |
Document type | Journal Article ; Research Support, Non-U.S. Gov't |
ZDB-ID | 209104-5 |
ISSN | 1091-6490 ; 0027-8424 |
ISSN (online) | 1091-6490 |
ISSN | 0027-8424 |
DOI | 10.1073/pnas.0610716104 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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