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Article ; Online: M-COPA, a novel Golgi system disruptor, suppresses apoptosis induced by Shiga toxin.

Hattori, Takayuki / Watanabe-Takahashi, Miho / Shiina, Isamu / Ohashi, Yoshimi / Dan, Shingo / Nishikawa, Kiyotaka / Yamori, Takao / Naito, Mikihiko

Genes to cells : devoted to molecular & cellular mechanisms

2016 Volume 21, Issue 8, Page(s) 901–906

Abstract: ... that 2-methylcoprophilinamide (M-COPA), a compound that induces disassembly of the Golgi apparatus ... by inactivating ADP-ribosylation factor 1 (Arf1), suppresses Stx-induced apoptosis. M-COPA inhibited transport ... by M-COPA could be a novel approach to suppress Stx toxicity. ...

Abstract	Shiga toxin (Stx) is a main virulence factor of Stx-producing Escherichia coli (STEC) that contributes to diarrhea and hemorrhagic colitis and occasionally to fatal systemic complications. Therefore, the development of an antidote to neutralize Stx toxicity is urgently needed. After internalization into cells, Stx is transferred to the Golgi apparatus via a retrograde vesicular transport system. We report here that 2-methylcoprophilinamide (M-COPA), a compound that induces disassembly of the Golgi apparatus by inactivating ADP-ribosylation factor 1 (Arf1), suppresses Stx-induced apoptosis. M-COPA inhibited transport of Stx from the plasma membrane to the Golgi apparatus and suppressed degradation of anti-apoptotic proteins and the activation of caspases. These findings suggest that inhibition of Stx retrograde transport by M-COPA could be a novel approach to suppress Stx toxicity.
MeSH term(s)	ADP-Ribosylation Factor 1/antagonists & inhibitors ; ADP-Ribosylation Factor 1/genetics ; Alkenes/chemistry ; Alkenes/pharmacology ; Antidotes/chemistry ; Antidotes/pharmacology ; Apoptosis/drug effects ; Apoptosis/genetics ; Colitis/drug therapy ; Colitis/microbiology ; Diarrhea/drug therapy ; Diarrhea/microbiology ; Golgi Apparatus/drug effects ; Humans ; Naphthols/administration & dosage ; Pyridines/administration & dosage ; Shiga Toxin/antagonists & inhibitors ; Shiga Toxin/toxicity ; Shiga-Toxigenic Escherichia coli/drug effects ; Shiga-Toxigenic Escherichia coli/pathogenicity
Chemical Substances	Alkenes ; Antidotes ; N-(pyridine-3-ylmethyl)-5-(7-hydroxy-2,6,8-trimethyl-1,2,4a,5,6,7,8,8a-octahydronaphthalene-1-yl)-2-methylpenta-2,4-dienamide ; Naphthols ; Pyridines ; coprophilin ; Shiga Toxin (75757-64-1) ; ADP-Ribosylation Factor 1 (EC 3.6.5.2)
Language	English
Publishing date	2016-08
Publishing country	England
Document type	Journal Article
ZDB-ID	1330000-3
ISSN	1365-2443 ; 1356-9597
ISSN (online)	1365-2443
ISSN	1356-9597
DOI	10.1111/gtc.12386
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Zs.A 4539: Show issues

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Article ; Online: Determination of trace aluminum by fluorescence quenching with m-carboxyphenylfluorone as analytical reagent.

Hoshino, Mitsuru / Kamino, Shinichiro / Takada, Shingo / Ijyuin, Megumi / Nakanishi, Maki / Naito, Masahito / Asano, Mamiko / Yamaguchi, Takako / Fujita, Yoshikazu

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry

2011 Volume 27, Issue 6, Page(s) 659–662

Abstract: ... This method involves a fluorescence quenching reaction that results in the formation of an m ...

Abstract	An improved method for the fluorophotometric determination of trace Al(III) has been developed. This method involves a fluorescence quenching reaction that results in the formation of an m-carboxyphenylfluorone-Al(III) complex in a poly(N-vinylpyrrolidone) micellar medium. The calibration curve was found to be linear in the range of 0.03-1.50 µg dm(-3). We successfully applied the proposed method to an assay of Al(III) in canned beverages, which required only sample dilution and no sample pretreatment. The proposed method is expected to determine Al(III) in a simple and rapid manner.
MeSH term(s)	Aluminum/analysis ; Beverages/analysis ; Fluoresceins/chemistry ; Fluorescence ; Micelles
Chemical Substances	4-carboxyphenylfluorone ; Fluoresceins ; Micelles ; Aluminum (CPD4NFA903)
Language	English
Publishing date	2011-05-17
Publishing country	Japan
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1483376-1
ISSN	1348-2246 ; 0910-6340
ISSN (online)	1348-2246
ISSN	0910-6340
DOI	10.2116/analsci.27.659
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Article ; Online: The effect of 30-Gy X-ray irradiation on platelets washed with M-sol additive solution.

Hirayama, Junichi / Fujihara, Mitsuhiro / Akino, Mitsuaki / Naito, Yu / Hayashi, Yoshiaki / Homma, Chihiro / Kato, Toshiaki / Ikeda, Hisami / Takamoto, Shigeru

Transfusion

2014 Volume 54, Issue 7, Page(s) 1904–1906

MeSH term(s)	Blood Platelets/drug effects ; Blood Platelets/radiation effects ; Blood Preservation/adverse effects ; Blood Preservation/methods ; Graft vs Host Disease/prevention & control ; Humans ; Organ Preservation Solutions/pharmacology ; Quality Control ; Radiation Dosage ; X-Rays
Chemical Substances	Organ Preservation Solutions
Language	English
Publishing date	2014-07
Publishing country	United States
Document type	Evaluation Studies ; Letter
ZDB-ID	208417-x
ISSN	1537-2995 ; 0041-1132
ISSN (online)	1537-2995
ISSN	0041-1132
DOI	10.1111/trf.12667
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Zs.A 284: Show issues

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Article ; Online: Stable π-π dependent electron conduction band of TPP[M(Pc)L2]2 molecular conductors (TPP = tetraphenylphosphonium; M = Co, Fe; Pc = phthalocyaninato; L = CN, Cl, Br).

Yu, Derrick Ethelbhert C / Matsuda, Masaki / Tajima, Hiroyuki / Naito, Toshio / Inabe, Tamotsu

Dalton transactions (Cambridge, England : 2003)

2011 Volume 40, Issue 10, Page(s) 2283–2288

Abstract: The partially-oxidized TPP[M(Pc)L(2)](2) molecular conductors exhibit variable electronic and ... in the intra-molecular π-d (Pc-M) and inter-molecular π-π (Pc-Pc) interactions in the TPP[M(Pc)L(2)](2) system ... materials properties of the TPP[M(Pc)L(2)](2) molecular conductors. ...

Abstract	The partially-oxidized TPP[M(Pc)L(2)](2) molecular conductors exhibit variable electronic and magnetic transport bulk materials properties due to central metal and axial ligand molecular modifications. The controllable electrical conductivity and giant negative magnetoresistance can be mainly attributable to the varying ligand field energy and physical bulkiness of the axial ligands which cause modulation in the intra-molecular π-d (Pc-M) and inter-molecular π-π (Pc-Pc) interactions in the TPP[M(Pc)L(2)](2) system, respectively. Characterization of the electronic conduction band utilizing one-dimensional (1-D) tight-binding approximation from infrared reflectance and thermoelectric power profile reveal consistent band widths of 0.43 eV-0.62 eV for the Co series (L = Br < Cl < CN) and 0.44-0.56 eV for the Fe series (L = Br < Cl < CN). The fixed band width suggests that stable electron conduction bands (transport pathway) can be constructed which can withstand the molecular π-d interaction modifications that severely alter the bulk electronic and magnetic materials properties of the TPP[M(Pc)L(2)](2) molecular conductors.
Language	English
Publishing date	2011-03-14
Publishing country	England
Document type	Journal Article
ZDB-ID	1472887-4
ISSN	1477-9234 ; 1364-5447 ; 0300-9246 ; 1477-9226
ISSN (online)	1477-9234 ; 1364-5447
ISSN	0300-9246 ; 1477-9226
DOI	10.1039/c0dt01054e
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Article ; Online: Interferon-α acts on the S/G2/M phases to induce apoptosis in the G1 phase of an IFNAR2-expressing hepatocellular carcinoma cell line.

Maeda, Sakae / Wada, Hiroshi / Naito, Yoko / Nagano, Hiroaki / Simmons, Szandor / Kagawa, Yoshinori / Naito, Atsushi / Kikuta, Junichi / Ishii, Taeko / Tomimaru, Yoshito / Hama, Naoki / Kawamoto, Koichi / Kobayashi, Shogo / Eguchi, Hidetoshi / Umeshita, Koji / Ishii, Hideshi / Doki, Yuichiro / Mori, Masaki / Ishii, Masaru

The Journal of biological chemistry

2014 Volume 289, Issue 34, Page(s) 23786–23795

Abstract: ... biochemical assays demonstrated that the IFN-α/IFNAR2 axis sensitizes cells to apoptosis in the S/G2/M phases ...

Abstract	Interferon-α (IFN-α) is used clinically to treat hepatocellular carcinoma (HCC), although the detailed therapeutic mechanisms remain elusive. In particular, IFN-α has long been implicated in control of the cell cycle, but its actual point of action has not been clarified. Here, using time lapse imaging analyses of the human HCC cell line HuH7 carrying a fluorescence ubiquitination-based cell cycle indicator (Fucci), we found that IFN-α induced cell cycle arrest in the G0/G1 phases, leading to apoptosis through an IFN-α type-2 receptor (IFNAR2)-dependent signaling pathway. Detailed analyses by time lapse imaging and biochemical assays demonstrated that the IFN-α/IFNAR2 axis sensitizes cells to apoptosis in the S/G2/M phases in preparation for cell death in the G0/G1 phases. In summary, this study is the first to demonstrate the detailed mechanism of IFN-α as an anticancer drug, using Fucci-based time lapse imaging, which will be informative for treating HCC with IFN-α in clinical practice.
MeSH term(s)	Blotting, Western ; Carcinoma, Hepatocellular/metabolism ; Carcinoma, Hepatocellular/pathology ; Cell Cycle/drug effects ; Cell Line, Tumor ; DNA Primers ; Flow Cytometry ; Humans ; Interferon-alpha/pharmacology ; Liver Neoplasms/metabolism ; Liver Neoplasms/pathology ; Real-Time Polymerase Chain Reaction ; Receptor, Interferon alpha-beta/metabolism
Chemical Substances	DNA Primers ; IFNAR2 protein, human ; Interferon-alpha ; Receptor, Interferon alpha-beta (156986-95-7)
Language	English
Publishing date	2014-07-10
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2997-x
ISSN	1083-351X ; 0021-9258
ISSN (online)	1083-351X
ISSN	0021-9258
DOI	10.1074/jbc.M114.551879
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Uc I Zs.108: Show issues

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Book ; Online: GENERATION OF THE 30 M-MESH GLOBAL DIGITAL SURFACE MODEL BY ALOS PRISM

Tadono, T. / Nagai, H. / Ishida, H. / Oda, F. / Naito, S. / Minakawa, K. / Iwamoto, H.

eISSN: 2194-9034

2016

Abstract: ... and its dataset consists of the global DSM dataset with 0.15 arcsec. pixel spacing (approx. 5 m mesh ... and ortho-rectified PRISM image with 2.5 m resolution. JAXA is also processing the global DSM with 1 ... arcsec. spacing (approx. 30 m mesh) based on the AW3D DSM dataset, and partially releasing it free ...

Abstract	Topographical information is fundamental to many geo-spatial related information and applications on Earth. Remote sensing satellites have the advantage in such fields because they are capable of global observation and repeatedly. Several satellite-based digital elevation datasets were provided to examine global terrains with medium resolutions e.g. the Shuttle Radar Topography Mission (SRTM), the global digital elevation model by the Advanced Spaceborne Thermal Emission and Reflection Radiometer (ASTER GDEM). A new global digital surface model (DSM) dataset using the archived data of the Panchromatic Remote-sensing Instrument for Stereo Mapping (PRISM) onboard the Advanced Land Observing Satellite (ALOS, nicknamed “Daichi”) has been completed on March 2016 by Japan Aerospace Exploration Agency (JAXA) collaborating with NTT DATA Corp. and Remote Sensing Technology Center, Japan. This project is called “ALOS World 3D” (AW3D), and its dataset consists of the global DSM dataset with 0.15 arcsec. pixel spacing (approx. 5 m mesh) and ortho-rectified PRISM image with 2.5 m resolution. JAXA is also processing the global DSM with 1 arcsec. spacing (approx. 30 m mesh) based on the AW3D DSM dataset, and partially releasing it free of charge, which calls “ALOS World 3D 30 m mesh” (AW3D30). The global AW3D30 dataset will be released on May 2016. This paper describes the processing status, a preliminary validation result of the AW3D30 DSM dataset, and its public release status. As a summary of the preliminary validation of AW3D30 DSM, 4.40 m (RMSE) of the height accuracy of the dataset was confirmed using 5,121 independent check points distributed in the world.
Subject code	910
Language	English
Publishing date	2016-06-13
Publishing country	de
Document type	Book ; Online
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Article ; Online: Feasibility re-evaluation of 75 mg/m² docetaxel in Japanese patients with previously treated non-small cell lung cancer.

Yamada, Kazuhiko / Takeoka, Hiroaki / Mizoguchi, Yusuke / Yamashita, Fumie / Yoshida, Tsukasa / Zaizen, Yoshiaki / Okayama, Yusuke / Naito, Yoshiko / Azuma, Koichi / Hoshino, Tomoaki

Japanese journal of clinical oncology

2014 Volume 44, Issue 4, Page(s) 338–345

Abstract: ... at doses of up to 75 mg/m² in Japanese patients with previously treated non-small cell lung cancer ... Methods: Patients received escalated doses of docetaxel at 70 mg/m² (level 1) or 75 mg/m² (level 2 ... confidence interval 1.4-6.6 months).: Conclusions: Although docetaxel administration at an initial dose of 75 mg/m² ...

Abstract	Objective: The primary objective of this study was to re-evaluate the feasibility of docetaxel at doses of up to 75 mg/m² in Japanese patients with previously treated non-small cell lung cancer. Methods: Patients received escalated doses of docetaxel at 70 mg/m² (level 1) or 75 mg/m² (level 2) every 3 weeks until disease progression or unacceptable toxicities. Dose escalation was decided on the basis of dose-limiting toxicity in the first cycle of chemotherapy. Results: At dose level 1, dose-limiting toxicity--Grade 3 febrile neutropenia--was observed in one of the six patients and at dose level 2, it was seen in one of the first six patients. Therefore, an additional 14 patients were enrolled at dose level 2, as originally planned. Among the total of 20 patients at dose level 2, 6 (<33%) developed dose-limiting toxicity in the first cycle: febrile neutropenia in 5 and pneumonia in 1. Finally, 10 (50%) of the 20 patients experienced toxicities that met the dose-limiting toxicity criteria, including 8 with febrile neutropenia throughout the treatment period, but this was manageable with dose reduction or appropriate supportive care. Other observed toxicities were predictable from the safety profile of decetaxel and were also well managed. Four partial responses were observed, giving an overall response rate of 15.4%. The median progression-free survival period of the patients overall was 4.0 months (95% confidence interval 1.4-6.6 months). Conclusions: Although docetaxel administration at an initial dose of 75 mg/m² requires careful attention because of the high incidence of febrile neutropenia, this dose is considered feasible according to the protocol definition in Japanese patients with previously treated non-small cell lung cancer.
MeSH term(s)	Adult ; Aged ; Antineoplastic Agents/administration & dosage ; Antineoplastic Agents/adverse effects ; Asian Continental Ancestry Group/statistics & numerical data ; Carcinoma, Non-Small-Cell Lung/drug therapy ; Disease Progression ; Disease-Free Survival ; Drug Administration Schedule ; Feasibility Studies ; Febrile Neutropenia/chemically induced ; Female ; Humans ; Japan/epidemiology ; Kaplan-Meier Estimate ; Lung Neoplasms/drug therapy ; Male ; Middle Aged ; Patient Selection ; Retreatment/methods ; Taxoids/administration & dosage ; Taxoids/adverse effects ; Treatment Outcome
Chemical Substances	Antineoplastic Agents ; Taxoids ; docetaxel (15H5577CQD)
Language	English
Publishing date	2014-04
Publishing country	England
Document type	Comparative Study ; Journal Article
ZDB-ID	190978-2
ISSN	1465-3621 ; 0368-2811
ISSN (online)	1465-3621
ISSN	0368-2811
DOI	10.1093/jjco/hyt236
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Zs.A 1298: Show issues

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Article: Phylogenic analysis of the M genes of influenza viruses isolated from free-flying water birds from their Northern Territory to Hokkaido, Japan.

Manzoor, Rashid / Sakoda, Yoshihiro / Mweene, Aaron / Tsuda, Yoshimi / Kishida, Noriko / Bai, Gui-Rong / Kameyama, Ken-Ichiro / Isoda, Norikazu / Soda, Kosuke / Naito, Michiko / Kida, Hiroshi

Virus genes

2008 Volume 37, Issue 2, Page(s) 144–152

Abstract: ... Japan. Phylogenic analysis of the matrix (M) genes of 67 strains, selected on the basis of their subtype ... combinations, revealed that A/duck/Hokkaido/W95/2006 (H10N8) was a reassortant whose M and NA genes [corrected ... lineages. The M genes of other 65 strains belonged to Eurasian non-gull-avian and the one to Eurasian-gull ...

Abstract	During 2000-2007, 218 influenza viruses of 28 different combinations of HA (H1-H13) and NA (N1-N9) subtypes were isolated from fecal samples of free-flying water birds at two distant lakes in Hokkaido, Japan. Phylogenic analysis of the matrix (M) genes of 67 strains, selected on the basis of their subtype combinations, revealed that A/duck/Hokkaido/W95/2006 (H10N8) was a reassortant whose M and NA genes [corrected] belonged to North American non-gull-avian and the other six [corrected] genes to Eurasian non-gull-avian lineages. The M genes of other 65 strains belonged to Eurasian non-gull-avian and the one to Eurasian-gull lineages. The M genes of 65 strains were grouped into three different sublineages, indicating that influenza viruses circulating in different populations of free-flying water birds have evolved independently in nature.
MeSH term(s)	Animal Migration ; Animals ; Animals, Wild/virology ; Birds/virology ; Ducks ; Evolution, Molecular ; Feces/virology ; Influenza A virus/classification ; Influenza A virus/genetics ; Influenza A virus/isolation & purification ; Japan ; Molecular Sequence Data ; Northern Territory ; Phylogeny ; Reassortant Viruses/classification ; Reassortant Viruses/genetics ; Reassortant Viruses/isolation & purification ; Viral Matrix Proteins/genetics ; Viral Matrix Proteins/metabolism
Chemical Substances	M1 protein, Influenza A virus ; Viral Matrix Proteins
Language	English
Publishing date	2008-07-10
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	639496-6
ISSN	1572-994X ; 0920-8569
ISSN (online)	1572-994X
ISSN	0920-8569
DOI	10.1007/s11262-008-0248-7
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Zs.A 2397: Show issues

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Article ; Online: Electrical resistivity anomaly in (Pr1−yMy)1−xCaxCoO3 epitaxial films (M=Y, Gd) fabricated by pulsed laser deposition

Y. Noda / H. Fujishiro / T. Naito / A. Ito / T. Goto / J. Hejtmanek / Z. Jirak

AIP Advances, Vol 6, Iss 2, Pp 025318-025318-

2016 Volume 9

Abstract: Pr1−yMy)1−xCaxCoO3 epitaxial films (M=Y, Gd) have been successfully fabricated by pulsed laser ...

Abstract	(Pr1−yMy)1−xCaxCoO3 epitaxial films (M=Y, Gd) have been successfully fabricated by pulsed laser deposition on the single crystal substrates with different lattice constant. The polycrystalline bulk of this material shows a first-order metal-insulator (MI) transition below the critical temperature. Although ρ(T) of all the as-grown films shows a semiconducting behavior at entire temperature range, an anomalous ρ(T) upturn with wide hysteresis can be clearly seen for the film grown on the SrLaAlO4 (SLAO) substrate, which applied the in-plane compressive stress to the film. Such anomaly in ρ(T) is interpreted as a sign of the first-order phase transition related with the spin-state (SS) transition, which was observed in the polycrystalline bulk.
Keywords	Science ; Q ; Science (General) ; Q1-390 ; Physics ; QC1-999
Language	English
Publishing date	2016-02-01T00:00:00Z
Publisher	AIP Publishing LLC
Document type	Article ; Online
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Article: Maturation/M-phase promoting factor: a regulator of aging in porcine oocytes.

Kikuchi, K / Naito, K / Noguchi, J / Shimada, A / Kaneko, H / Yamashita, M / Aoki, F / Tojo, H / Toyoda, Y

Biology of reproduction

2000 Volume 63, Issue 3, Page(s) 715–722

Abstract: ... we showed that porcine aged oocytes had low maturation/M-phase promoting factor (MPF) activity, and ...

Abstract	Deterioration in the quality of mammalian oocytes during the metaphase-II arrest period is well known as "oocyte aging." Oocytes in which aging has occurred are called aged oocytes, and these oocytes show enhanced activation and higher fragmentation rates after parthenogenetic activation. Previously we showed that porcine aged oocytes had low maturation/M-phase promoting factor (MPF) activity, and we suggested that this low MPF activity contributed at least in part to the aging phenomena. In the present study, we examined the relationship between MPF activity and these aging phenomena by artificially regulating MPF activity in porcine metaphase-II-arrested oocytes. Since we have shown recently that aged porcine oocytes contain abundant phosphorylated inactive MPF, so-called pre-MPF, we used vanadate and caffeine, which affect the phosphorylation status of MPF, to regulate MPF activity. Incubation of 48-h-matured oocytes with vanadate for 1 h increased the phosphorylation of MPF and decreased MPF activity. The parthenogenetic activation and fragmentation rates were significantly increased compared with those of control oocytes. Conversely, treatment of 72-h-cultured aged oocytes with caffeine (last 10 h of culture) decreased the level of pre-MPF and elevated MPF activity. These oocytes revealed significantly lower parthenogenetic activation rates and a lower percentage of fragmentation than did untreated aged oocytes. These results indicate that not only the increased ability for parthenogenetic activation but also the increased fragmentation rate observed in porcine aged oocytes may be attributable in part to the gradual decrease in MPF activity during prolonged culture. Control of MPF phosphorylation with these agents may allow for some degree of manipulation of oocyte aging.
MeSH term(s)	Animals ; CDC2 Protein Kinase/metabolism ; Caffeine/pharmacology ; Cells, Cultured ; Cellular Senescence/drug effects ; Female ; Histones/metabolism ; Maturation-Promoting Factor/physiology ; Meiosis ; Metaphase ; Oocytes/physiology ; Phosphorylation ; Swine ; Time Factors ; Vanadates/pharmacology
Chemical Substances	Histones ; Caffeine (3G6A5W338E) ; Vanadates (3WHH0066W5) ; CDC2 Protein Kinase (EC 2.7.11.22) ; Maturation-Promoting Factor (EC 2.7.11.22)
Language	English
Publishing date	2000-09
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1118-6
ISSN	1529-7268 ; 0006-3363
ISSN (online)	1529-7268
ISSN	0006-3363
DOI	10.1095/biolreprod63.3.715
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