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  1. Article: Immobilized cellulose nanospheres enable rapid antigen detection in lateral flow immunoassays.

    Solin, Katariina / Beaumont, Marco / Borghei, Maryam / Orelma, Hannes / Mertens, Pascal / Rojas, Orlando J

    Cellulose (London, England)

    2023  Volume 30, Issue 4, Page(s) 2353–2365

    Language English
    Publishing date 2023-01-05
    Publishing country England
    Document type Journal Article
    ZDB-ID 1496831-9
    ISSN 1572-882X ; 0969-0239
    ISSN (online) 1572-882X
    ISSN 0969-0239
    DOI 10.1007/s10570-022-05038-y
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  2. Article ; Online: EAACI task force report: A consensus protocol for the basophil activation test for collaboration and external quality assurance.

    Pascal, M / Edelman, S M / Nopp, A / Möbs, C / Geilenkeuser, W J / Knol, E F / Ebo, D G / Mertens, C / Shamji, M H / Santos, A F / Patil, S / Eberlein, B / Mayorga, C / Hoffmann, H J

    Allergy

    2023  Volume 79, Issue 2, Page(s) 290–293

    MeSH term(s) Humans ; Basophil Degranulation Test ; Consensus ; Basophils
    Language English
    Publishing date 2023-10-05
    Publishing country Denmark
    Document type Journal Article
    ZDB-ID 391933-x
    ISSN 1398-9995 ; 0105-4538
    ISSN (online) 1398-9995
    ISSN 0105-4538
    DOI 10.1111/all.15907
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Immobilized cellulose nanospheres enable rapid antigen detection in lateral flow immunoassays

    Solin, Katariina / Beaumont, Marco / Borghei, Maryam / Orelma, Hannes / Mertens, Pascal / Rojas, Orlando J.

    Cellulose. 2023 Mar., v. 30, no. 4 p.2353-2365

    2023  

    Abstract: Rapid diagnostic systems are essential in controlling the spread of viral pathogens and efficient patient management. The available technologies for low-cost viral antigen testing have several limitations, including a lack of accuracy and sensitivity. ... ...

    Abstract Rapid diagnostic systems are essential in controlling the spread of viral pathogens and efficient patient management. The available technologies for low-cost viral antigen testing have several limitations, including a lack of accuracy and sensitivity. Here, we introduce a platform based on cellulose II nanoparticles (oppositely charged NPan and NPcat) for effective control of surface protein interactions, leading to rapid and sensitive antigen tests. Passivation against non-specific adsorption and augmented immobilization of sensing antibodies is achieved by adjusting the electrostatic charge of the nanoparticles. The interactions affecting the performance of the system are investigated by microgravimetry and confocal imaging. As a proof-of-concept test, SARS-CoV-2 nucleocapsid sensing was carried out by using saliva-wicking by channels that were stencil-printed on paper. We conclude that inkjet-printed NPcat elicits strong optical signals, visible after a few minutes, opening the opportunity for cost-effective and rapid diagnostic.
    Keywords Severe acute respiratory syndrome coronavirus 2 ; adsorption ; antigen detection ; cellulose ; cost effectiveness ; electrostatic interactions ; nanoparticles ; nanospheres ; nucleocapsid ; patients ; surface proteins ; viral antigens
    Language English
    Dates of publication 2023-03
    Size p. 2353-2365.
    Publishing place Springer Netherlands
    Document type Article ; Online
    ZDB-ID 1496831-9
    ISSN 1572-882X ; 0969-0239
    ISSN (online) 1572-882X
    ISSN 0969-0239
    DOI 10.1007/s10570-022-05038-y
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  4. Article: Hypoglycaemia after Initiation of CFTR Modulator Therapy in a Cystic Fibrosis Patient without Diabetes.

    Yskout, Marie / Vliebergh, Joke / Bor, Hakan / Dupont, Lieven / Lorent, Natalie / Van Bleyenbergh, Pascal / Gillard, Pieter / Van der Schueren, Bart / Mertens, Ann / Mathieu, Chantal / Vangoitsenhoven, Roman

    Case reports in endocrinology

    2023  Volume 2023, Page(s) 9769119

    Abstract: Introduction: Cystic fibrosis transmembrane regulator (CFTR) modulator therapies improve respiratory function and glycaemic control in patients with cystic fibrosis (CF). The direct effect of CFTR modulator therapies on pancreatic function in patients ... ...

    Abstract Introduction: Cystic fibrosis transmembrane regulator (CFTR) modulator therapies improve respiratory function and glycaemic control in patients with cystic fibrosis (CF). The direct effect of CFTR modulator therapies on pancreatic function in patients without preexisting diabetes remains unclear.
    Conclusion: Treatment of CF patients without diabetes with CFTR modulator therapies can cause recurrent hypoglycaemic episodes which resolve with dietary measures.
    Language English
    Publishing date 2023-12-23
    Publishing country United States
    Document type Case Reports
    ZDB-ID 2627633-1
    ISSN 2090-651X ; 2090-6501
    ISSN (online) 2090-651X
    ISSN 2090-6501
    DOI 10.1155/2023/9769119
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  5. Article ; Online: Development of an immunochromatographic lateral flow assay to rapidly detect OXA-23-, OXA-40-, OXA-58- and NDM-mediated carbapenem resistance determinants in

    Mertins, Sonja / Higgins, Paul G / Thunissen, Caroline / Magein, Henri / Gilleman, Quentin / Mertens, Pascal / Rodríguez, María González / Maus, Liza Marie / Seifert, Harald / Krönke, Martin / Klimka, Alexander

    Journal of medical microbiology

    2023  Volume 72, Issue 4

    Abstract: Introduction. ...

    Abstract Introduction.
    MeSH term(s) Humans ; Acinetobacter baumannii ; Microbial Sensitivity Tests ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; beta-Lactamases/metabolism ; Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/therapeutic use ; Carbapenems/pharmacology ; Carbapenems/therapeutic use ; Immunoassay/methods
    Chemical Substances Bacterial Proteins ; beta-Lactamases (EC 3.5.2.6) ; Anti-Bacterial Agents ; Carbapenems
    Language English
    Publishing date 2023-04-12
    Publishing country England
    Document type Journal Article
    ZDB-ID 218356-0
    ISSN 1473-5644 ; 0022-2615
    ISSN (online) 1473-5644
    ISSN 0022-2615
    DOI 10.1099/jmm.0.001681
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    In stock of ZB MED Cologne/Königswinter

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    Jg. 1995 - 2021: Lesesall (1.OG)
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  6. Article ; Online: lab.js: A free, open, online study builder.

    Henninger, Felix / Shevchenko, Yury / Mertens, Ulf K / Kieslich, Pascal J / Hilbig, Benjamin E

    Behavior research methods

    2021  Volume 54, Issue 2, Page(s) 556–573

    Abstract: Web-based data collection is increasingly popular in both experimental and survey-based research because it is flexible, efficient, and location-independent. While dedicated software for laboratory-based experimentation and online surveys is commonplace, ...

    Abstract Web-based data collection is increasingly popular in both experimental and survey-based research because it is flexible, efficient, and location-independent. While dedicated software for laboratory-based experimentation and online surveys is commonplace, researchers looking to implement experiments in the browser have, heretofore, often had to manually construct their studies' content and logic using code. We introduce lab.js, a free, open-source experiment builder that makes it easy to build studies for both online and in-laboratory data collection. Through its visual interface, stimuli can be designed and combined into a study without programming, though studies' appearance and behavior can be fully customized using HTML, CSS, and JavaScript code if required. Presentation and response times are kept and measured with high accuracy and precision heretofore unmatched in browser-based studies. Experiments constructed with lab.js can be run directly on a local computer and published online with ease, with direct deployment to cloud hosting, export to web servers, and integration with popular data collection platforms. Studies can also be shared in an editable format, archived, re-used and adapted, enabling effortless, transparent replications, and thus facilitating open, cumulative science. The software is provided free of charge under an open-source license; further information, code, and extensive documentation are available from https://lab.js.org/ .
    MeSH term(s) Computers ; Data Collection ; Humans ; Reaction Time ; Software
    Language English
    Publishing date 2021-07-28
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 231560-9
    ISSN 1554-3528 ; 0743-3808 ; 1554-351X
    ISSN (online) 1554-3528
    ISSN 0743-3808 ; 1554-351X
    DOI 10.3758/s13428-019-01283-5
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    In stock of ZB MED Bonn / Germany

    Z 76/512: Show issues

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  7. Article ; Online: Evaluation of the rSP03B sero-strip, a newly proposed rapid test for canine exposure to Phlebotomus perniciosus, vector of Leishmania infantum.

    Willen, Laura / Mertens, Pascal / Volf, Petr

    PLoS neglected tropical diseases

    2018  Volume 12, Issue 8, Page(s) e0006607

    Abstract: Background: Canine leishmaniasis (CanL) is a zoonotic disease, caused by Leishmania infantum and transmitted by Phlebotomus perniciosus in the Mediterranean basin. Previously, an ELISA based on the P. perniciosus salivary protein SP03B was proposed as a ...

    Abstract Background: Canine leishmaniasis (CanL) is a zoonotic disease, caused by Leishmania infantum and transmitted by Phlebotomus perniciosus in the Mediterranean basin. Previously, an ELISA based on the P. perniciosus salivary protein SP03B was proposed as a valid tool to screen for canine exposure to sand fly bites across regions endemic for CanL. Although this approach is useful in laboratory settings, a practical tool for immediate application in the field is needed. In this study we propose the rSP03B sero-strip, the first immunochromatographic test (ICT) in the field of vector exposure able to rapidly screen dogs living in endemic areas for the presence of P. perniciosus and to aid in the evaluation of vector control programs.
    Methodology/principal findings: The ICT was prepared using the bacterially expressed recombinant protein rSP03B as antigen. For test optimization, pre-immune sera from non-bitten laboratory-bred Beagles were used as negative controls. In order to validate the test, sera from laboratory-bred Beagles experimentally exposed to P. perniciosus bites were used as positive controls. Additionally, all samples were tested by ELISA using whole salivary gland homogenate (SGH) and the rSP03B protein as antigen. An almost perfect degree of agreement was found between the ICT and the SGH-ELISA. Furthermore, the newly proposed rSP03B sero-strip showed a sensitivity of 100% and a specificity of 86.79%.
    Conclusions/significance: We developed a simple and rapid ICT based on the P. perniciosus rSP03B salivary protein, able to replace the standard ELISA used in previous studies. Our rSP03B sero-strip showed to be highly sensitive and specific in the detection of antibodies (IgG) against P. perniciosus saliva. In the future, this test can be employed during large-scale epidemiological studies of CanL in the Mediterranean area to evaluate the efficacy of vector control programs.
    MeSH term(s) Animals ; Chromatography, Affinity/veterinary ; Dog Diseases/diagnosis ; Dog Diseases/parasitology ; Dogs ; Enzyme-Linked Immunosorbent Assay/methods ; Insect Bites and Stings/immunology ; Insect Bites and Stings/veterinary ; Insect Proteins ; Insect Vectors ; Leishmania infantum ; Phlebotomus/immunology ; Reagent Strips ; Sensitivity and Specificity ; Serologic Tests/veterinary ; Time Factors ; Zoonoses
    Chemical Substances Insect Proteins ; Reagent Strips
    Language English
    Publishing date 2018-08-02
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2429704-5
    ISSN 1935-2735 ; 1935-2727
    ISSN (online) 1935-2735
    ISSN 1935-2727
    DOI 10.1371/journal.pntd.0006607
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Evaluation of the rSP03B sero-strip, a newly proposed rapid test for canine exposure to Phlebotomus perniciosus, vector of Leishmania infantum.

    Laura Willen / Pascal Mertens / Petr Volf

    PLoS Neglected Tropical Diseases, Vol 12, Iss 8, p e

    2018  Volume 0006607

    Abstract: BACKGROUND:Canine leishmaniasis (CanL) is a zoonotic disease, caused by Leishmania infantum and transmitted by Phlebotomus perniciosus in the Mediterranean basin. Previously, an ELISA based on the P. perniciosus salivary protein SP03B was proposed as a ... ...

    Abstract BACKGROUND:Canine leishmaniasis (CanL) is a zoonotic disease, caused by Leishmania infantum and transmitted by Phlebotomus perniciosus in the Mediterranean basin. Previously, an ELISA based on the P. perniciosus salivary protein SP03B was proposed as a valid tool to screen for canine exposure to sand fly bites across regions endemic for CanL. Although this approach is useful in laboratory settings, a practical tool for immediate application in the field is needed. In this study we propose the rSP03B sero-strip, the first immunochromatographic test (ICT) in the field of vector exposure able to rapidly screen dogs living in endemic areas for the presence of P. perniciosus and to aid in the evaluation of vector control programs. METHODOLOGY/PRINCIPAL FINDINGS:The ICT was prepared using the bacterially expressed recombinant protein rSP03B as antigen. For test optimization, pre-immune sera from non-bitten laboratory-bred Beagles were used as negative controls. In order to validate the test, sera from laboratory-bred Beagles experimentally exposed to P. perniciosus bites were used as positive controls. Additionally, all samples were tested by ELISA using whole salivary gland homogenate (SGH) and the rSP03B protein as antigen. An almost perfect degree of agreement was found between the ICT and the SGH-ELISA. Furthermore, the newly proposed rSP03B sero-strip showed a sensitivity of 100% and a specificity of 86.79%. CONCLUSIONS/SIGNIFICANCE:We developed a simple and rapid ICT based on the P. perniciosus rSP03B salivary protein, able to replace the standard ELISA used in previous studies. Our rSP03B sero-strip showed to be highly sensitive and specific in the detection of antibodies (IgG) against P. perniciosus saliva. In the future, this test can be employed during large-scale epidemiological studies of CanL in the Mediterranean area to evaluate the efficacy of vector control programs.
    Keywords Arctic medicine. Tropical medicine ; RC955-962 ; Public aspects of medicine ; RA1-1270
    Subject code 630
    Language English
    Publishing date 2018-08-01T00:00:00Z
    Publisher Public Library of Science (PLoS)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  9. Article ; Online: Correction: A lineage-specific rapid diagnostic test (Chagas Sero K-SeT) identifies Brazilian Trypanosoma cruzi II/V/VI reservoir hosts among diverse mammalian orders.

    McClean, Mairi C W / Bhattacharyya, Tapan / Mertens, Pascal / Murphy, Niamh / Gilleman, Quentin / Gustin, Yves / Zeippen, Nicolas / Xavier, Samanta C C / Jansen, Ana M / Miles, Michael A

    PloS one

    2020  Volume 15, Issue 4, Page(s) e0231566

    Abstract: This corrects the article DOI: 10.1371/journal.pone.0227828.]. ...

    Abstract [This corrects the article DOI: 10.1371/journal.pone.0227828.].
    Language English
    Publishing date 2020-04-02
    Publishing country United States
    Document type Published Erratum
    ISSN 1932-6203
    ISSN (online) 1932-6203
    DOI 10.1371/journal.pone.0231566
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  10. Article ; Online: A single nucleotide polymorphism in the gene for GPR183 increases its surface expression on blood lymphocytes of patients with inflammatory bowel disease.

    Ruiz, Florian / Wyss, Annika / Rossel, Jean-Benoît / Sulz, Michael Christian / Brand, Stephan / Moncsek, Anja / Mertens, Joachim C / Roth, René / Clottu, Aurélie S / Burri, Emanuel / Juillerat, Pascal / Biedermann, Luc / Greuter, Thomas / Rogler, Gerhard / Pot, Caroline / Misselwitz, Benjamin

    British journal of pharmacology

    2021  Volume 178, Issue 16, Page(s) 3157–3175

    MeSH term(s) Colitis ; Genetic Predisposition to Disease ; Genotype ; Humans ; Inflammatory Bowel Diseases/genetics ; Lymphocytes ; Polymorphism, Single Nucleotide ; Receptors, G-Protein-Coupled/genetics
    Chemical Substances GPR183 protein, human ; Receptors, G-Protein-Coupled
    Language English
    Publishing date 2021-03-10
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 80081-8
    ISSN 1476-5381 ; 0007-1188
    ISSN (online) 1476-5381
    ISSN 0007-1188
    DOI 10.1111/bph.15395
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