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Article ; Online: Centrifugo-Pneumatic Reciprocating Flowing Coupled with a Spatial Confinement Strategy for an Ultrafast Multiplexed Immunoassay.

Qian, Chungen / Li, Pengjie / Wang, Jingjing / Hong, Xianzhe / Zhao, Xudong / Wu, Liqiang / Miao, Zeyu / Du, Wei / Feng, Xiaojun / Li, Yiwei / Chen, Peng / Liu, Bi-Feng

Analytical chemistry

2024

Abstract: Immunoassays serve as powerful diagnostic tools for early disease screening, process monitoring, and precision treatment. However, the current methods are limited by high costs, prolonged processing times (>2 h), and operational complexities that hinder ... ...

Abstract	Immunoassays serve as powerful diagnostic tools for early disease screening, process monitoring, and precision treatment. However, the current methods are limited by high costs, prolonged processing times (>2 h), and operational complexities that hinder their widespread application in point-of-care testing. Here, we propose a novel centrifugo-
Language	English
Publishing date	2024-04-24
Publishing country	United States
Document type	Journal Article
ZDB-ID	1508-8
ISSN	1520-6882 ; 0003-2700
ISSN (online)	1520-6882
ISSN	0003-2700
DOI	10.1021/acs.analchem.4c00651
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Article ; Online: Antibody efficacy of inactivated vaccine boosters (CoronaVac) against Omicron variant from a 15-month follow-up study.

Yin, Yue / Li, Xinjie / Qian, Chungen / Cheng, Bangning / Lu, Fengmin / Shen, Tao

The Journal of infection

2022 Volume 85, Issue 4, Page(s) e119–e121

MeSH term(s)	Antibodies ; Antibodies, Neutralizing ; Antibodies, Viral ; COVID-19 Vaccines ; Follow-Up Studies ; Humans ; Immunization, Secondary ; Vaccines, Inactivated
Chemical Substances	Antibodies ; Antibodies, Neutralizing ; Antibodies, Viral ; COVID-19 Vaccines ; Vaccines, Inactivated
Language	English
Publishing date	2022-06-26
Publishing country	England
Document type	Letter ; Research Support, Non-U.S. Gov't ; Comment
ZDB-ID	424417-5
ISSN	1532-2742 ; 0163-4453
ISSN (online)	1532-2742
ISSN	0163-4453
DOI	10.1016/j.jinf.2022.06.018
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Article: Advances in Biosensors for Continuous Glucose Monitoring Towards Wearables.

Johnston, Lucy / Wang, Gonglei / Hu, Kunhui / Qian, Chungen / Liu, Guozhen

Frontiers in bioengineering and biotechnology

2021 Volume 9, Page(s) 733810

Abstract: Continuous glucose monitors (CGMs) for the non-invasive monitoring of diabetes are constantly being developed and improved. Although there are multiple biosensing platforms for monitoring glucose available on the market, there is still a strong need to ... ...

Abstract	Continuous glucose monitors (CGMs) for the non-invasive monitoring of diabetes are constantly being developed and improved. Although there are multiple biosensing platforms for monitoring glucose available on the market, there is still a strong need to enhance their precision, repeatability, wearability, and accessibility to end-users. Biosensing technologies are being increasingly explored that use different bodily fluids such as sweat and tear fluid, etc., that can be calibrated to and therefore used to measure blood glucose concentrations accurately. To improve the wearability of these devices, exploring different fluids as testing mediums is essential and opens the door to various implants and wearables that in turn have the potential to be less inhibiting to the wearer. Recent developments have surfaced in the form of contact lenses or mouthguards for instance. Challenges still present themselves in the form of sensitivity, especially at very high or low glucose concentrations, which is critical for a diabetic person to monitor. This review summarises advances in wearable glucose biosensors over the past 5 years, comparing the different types as well as the fluid they use to detect glucose, including the CGMs currently available on the market. Perspectives on the development of wearables for glucose biosensing are discussed.
Language	English
Publishing date	2021-08-19
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2719493-0
ISSN	2296-4185
ISSN	2296-4185
DOI	10.3389/fbioe.2021.733810
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Article: [Application of magnetic immunofluorescence assay based on microfluidic technology to detection of Epstein-Barr virus].

Li, Junhao / Han, Guanhua / Lin, Xiaotao / Wu, Liqiang / Qian, Chungen / Xu, Junfa

Se pu = Chinese journal of chromatography

2022 Volume 40, Issue 4, Page(s) 372–383

Abstract: Early diagnosis of Epstein-Barr virus (EBV) can reduce the risk of major illnesses. Disadvantages of EBV antibody detection methods that are commonly used clinically include lengthy assay time, need for a lot of reagent, and low efficiency. Compared with ...

Abstract	Early diagnosis of Epstein-Barr virus (EBV) can reduce the risk of major illnesses. Disadvantages of EBV antibody detection methods that are commonly used clinically include lengthy assay time, need for a lot of reagent, and low efficiency. Compared with traditional detection methods, microfluidics technology offers high throughput, low reagent consumption, less bio-contamination, and a higher degree of automation. Advantages of magnetic immunofluorescence technology include high detection efficiency and a strong signal. The combined advantages of the two methods can compensate for the shortcomings of traditional methods. In the present study, polymethyl methacrylate (PMMA) as the raw material was subjected to laser cutting and vacuum hot pressing to quickly obtain chips. Magnetic beads labeled with antigen and fluorescent microspheres labeled with anti-human antibody were then rapidly lyophilized into microspheres by freeze-drying and embedded into the chips. After incubation and cleaning, the last step was detection. Image J software was used to analyze the mean fluorescence intensity and obtain negative or positive test results. To determine the precision of the chip, high- and low-value samples of each item were retested 10 times. The mean values were calculated to obtain the relative standard deviation (RSD) for several common pathogens. Furthermore, the coincidence rate of clinical samples was tested using a chemiluminescence immunoassay (CLIA) to determine the potential clinical application value. The RSD of the precision test for each item was <10%, indicating good precision. The precision of the accelerated stability test was not verified. Specificity test results revealed no cross-reaction with some common pathogen antibodies, indicating good specificity. It remains to be verified whether the antibodies detected by this method cross-react with other herpes simplex viruses, such as types 1 and 2, Kaposi's sarcoma-associated virus, and human herpes virus type 6 and 7. Of the 121 clinical samples tested, statistical analysis of the data indicated good agreement with the chemiluminescence immunoassay in clinical trials. EB viral capsid antigen (EB VCA) IgG positive coincidence rate was 95.77% (68/71), the negative coincidence rate was 86% (43/50) (Kappa=0.828,
MeSH term(s)	Epstein-Barr Virus Infections/diagnosis ; Fluorescent Antibody Technique ; Herpesvirus 4, Human ; Humans ; Magnetic Phenomena ; Microfluidics ; Technology
Language	Chinese
Publishing date	2022-04-12
Publishing country	China
Document type	Journal Article
ISSN	1000-8713
ISSN	1000-8713
DOI	10.3724/SP.J.1123.2021.09005
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Article ; Online: On-Line Dual-Active Valves Based Centrifugal Microfluidic Chip for Fully Automated Point-of-Care Immunoassay

Qian, Chungen / Wan, Chao / Li, Shunji / Xiao, Yujin / Yuan, Huijuan / Gao, Siyu / Wu, Liqiang / Zhou, Mengfan / Feng, Xiaojun / Li, Yiwei / Chen, Peng / Liu, Bi-Feng

Analytical Chemistry. 2023 Aug. 09, v. 95, no. 33 p.12521-12531

2023

Abstract: There remains an unmet need for a fully integrated microfluidic platform that can automatically perform multistep and multireagent immunoassays. Here, we proposed a novel online dual-active valve-based centrifugal microfluidic chip, termed DAVM, for ... ...

Abstract	There remains an unmet need for a fully integrated microfluidic platform that can automatically perform multistep and multireagent immunoassays. Here, we proposed a novel online dual-active valve-based centrifugal microfluidic chip, termed DAVM, for fully automatic point-of-care immunoassay. Practically, the puncture valve, one of the dual active valves, is capable of achieving precise, on-demand, sequential release of prestored reagents, while the other valve-reversible active valve enables controlled retention and drainage of the reaction solutions. Thereby, our technology mitigates the challenges of hydrophilic/hydrophobic modifications and unstable valve control performance commonly observed in passive valve controls. As a proof of concept, the indirect enzymatic immunoblotting technique was employed on DAVM for fully automated immunological analysis of eight targets, yielding outcomes within an hour. Furthermore, we conducted a comparative analysis of 28 clinical samples with autoimmune diseases. According to 224 clinical data, the sample testing concordance rate between DAVM and the traditional instrument was 82%, with a target compliance rate of 97%. Therefore, our DAVM system has powerful potential for fully automated immunoassays.
Keywords	analytical chemistry ; automation ; compliance ; drainage ; hydrophilicity ; hydrophobicity ; immunoassays ; immunoblotting ; organ-on-a-chip ; point-of-care systems
Language	English
Dates of publication	2023-0809
Size	p. 12521-12531.
Publishing place	American Chemical Society
Document type	Article ; Online
ZDB-ID	1508-8
ISSN	1520-6882 ; 0003-2700
ISSN (online)	1520-6882
ISSN	0003-2700
DOI	10.1021/acs.analchem.3c02564
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Case Report: Recurrent Autoimmune Hypoglycemia Induced by Non-Hypoglycemic Medications.

Zhu, Qiuping / Zhao, Hanxin / Qiu, Wei / Wu, Fang / Qian, Chungen / Yang, Yonghong / Kang, Ye / Zheng, Fenping / Zhou, Jiaqiang

Frontiers in immunology

2022 Volume 13, Page(s) 855350

Abstract: We present a case of recurrent autoimmune hypoglycemia induced by non-hypoglycemic agents. We review reported cases of autoimmune hypoglycemia related to non-hypoglycemic agents, and discuss the effects of different detection methods for insulin ... ...

Abstract	We present a case of recurrent autoimmune hypoglycemia induced by non-hypoglycemic agents. We review reported cases of autoimmune hypoglycemia related to non-hypoglycemic agents, and discuss the effects of different detection methods for insulin autoantibodies on the results obtained. We aim to provide information for clinicians and a warning for medication usage. Considering the increasing number of clopidogrel-induced AIH cases and the hypoglycemia-induced increase in the risk of cardiovascular events, we recommend that cardiovascular disease patients being treated with clopidogrel be informed of this rare side effect and that clinicians be vigilant for the possibility of autoimmune hypoglycemia in this patient population.
MeSH term(s)	Autoimmune Diseases/diagnosis ; Autoimmune Diseases/drug therapy ; Clopidogrel/therapeutic use ; Humans ; Hypoglycemia/chemically induced ; Hypoglycemia/diagnosis ; Hypoglycemia/drug therapy ; Insulin ; Insulin Antibodies/therapeutic use
Chemical Substances	Insulin ; Insulin Antibodies ; Clopidogrel (A74586SNO7)
Language	English
Publishing date	2022-07-22
Publishing country	Switzerland
Document type	Case Reports ; Review
ZDB-ID	2606827-8
ISSN	1664-3224 ; 1664-3224
ISSN (online)	1664-3224
ISSN	1664-3224
DOI	10.3389/fimmu.2022.855350
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Article ; Online: Programmable Gravity Self-Driven Microfluidic Chip for Point-of-Care Multiplied Immunoassays.

Yuan, Huijuan / Wan, Chao / Wang, Xin / Li, Shunji / Xie, Han / Qian, Chungen / Du, Wei / Feng, Xiaojun / Li, Yiwei / Chen, Peng / Liu, Bi-Feng

Small (Weinheim an der Bergstrasse, Germany)

2023 , Page(s) e2310206

Abstract: Point-of-care testing (POCT) is experiencing a groundbreaking transformation with microfluidic chips, which offer precise fluid control and manipulation at the microscale. Nevertheless, chip design or operation for existing platforms is rather cumbersome, ...

Abstract	Point-of-care testing (POCT) is experiencing a groundbreaking transformation with microfluidic chips, which offer precise fluid control and manipulation at the microscale. Nevertheless, chip design or operation for existing platforms is rather cumbersome, with some even heavily depending on external drivers or devices, impeding their broader utilization. This study develops a unique programmable gravity self-driven microfluidic chip (PGSMC) capable of simultaneous multi-reagent sequential release, multi-target analysis, and multi-chip operation. All necessary reagents are introduced in a single step, and the process is initiated simply by flipping the PGSMC vertically, eliminating the need for additional steps or devices. Additionally, it demonstrates successful immunoassays in less than 60 min for antinuclear antibodies testing, compared to more than 120 min by traditional methods. Assessment using 25 clinically diagnosed cases showcases remarkable sensitivity (96%), specificity (100%), and accuracy (99%). These outcomes underscored its potential as a promising platform for POCT with high accuracy, speed, and reliability, highlighting its capability for automated fluid control.
Language	English
Publishing date	2023-12-12
Publishing country	Germany
Document type	Journal Article
ZDB-ID	2168935-0
ISSN	1613-6829 ; 1613-6810
ISSN (online)	1613-6829
ISSN	1613-6810
DOI	10.1002/smll.202310206
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Hand-powered centrifugal micropipette-tip with distance-based quantification for on-site testing of SARS-CoV-2 virus.

Qian, Chungen / Li, Jiashuo / Pang, Zheng / Xie, Han / Wan, Chao / Li, Shunji / Wang, Xin / Xiao, Yujin / Feng, Xiaojun / Li, Yiwei / Chen, Peng / Liu, Bi-Feng

Talanta

2023 Volume 258, Page(s) 124466

Abstract: This paper proposed a hand-powered centrifugal micropipette-tip strategy, termed HCM, for all-in-one immunoassay combined with a distance-based readout for portable quantitative detection of SARS-CoV-2. The target SARS-CoV-2 virus antigen triggers the ... ...

Abstract	This paper proposed a hand-powered centrifugal micropipette-tip strategy, termed HCM, for all-in-one immunoassay combined with a distance-based readout for portable quantitative detection of SARS-CoV-2. The target SARS-CoV-2 virus antigen triggers the binding of multiple monoclonal antibody-coated red latex nanobeads, forming larger complexes. Following incubation and centrifugation, the formed aggregated complexes settle at the bottom of the tip, while free red nanobeads remain suspended in the solution. The HCM enables sensitive (1 ng/mL) and reliable quantification of SARS-CoV-2 within 25 min. With the advantages of free washing, free fabrication, free instrument, and without the optical device, the proposed low-cost and easy-to-use HCM immunoassay shows great potential for quantitative POC diagnostics for SARS-CoV-2.
MeSH term(s)	Humans ; SARS-CoV-2 ; COVID-19/diagnosis ; Immunoassay
Language	English
Publishing date	2023-03-18
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1500969-5
ISSN	1873-3573 ; 0039-9140
ISSN (online)	1873-3573
ISSN	0039-9140
DOI	10.1016/j.talanta.2023.124466
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Article ; Online: Development of a rapid, sensitive detection method for SARS-CoV-2 and influenza virus based on recombinase polymerase amplification combined with CRISPR-Cas12a assay.

Wang, Yuning / Wu, Liqiang / Yu, Xiaomei / Wang, Gang / Pan, Ting / Huang, Zhao / Cui, Ting / Huang, Tianxun / Huang, Zhentao / Nie, Libo / Qian, Chungen

Journal of medical virology

2023 Volume 95, Issue 11, Page(s) e29215

Abstract: Respiratory tract infections are associated with the most common diseases transmitted among people and remain a huge threat to global public health. Rapid and sensitive diagnosis of causative agents is critical for timely treatment and disease control. ... ...

Abstract	Respiratory tract infections are associated with the most common diseases transmitted among people and remain a huge threat to global public health. Rapid and sensitive diagnosis of causative agents is critical for timely treatment and disease control. Here, we developed a novel method based on recombinase polymerase amplification (RPA) combined with CRISPR-Cas12a to detect three viral pathogens, including SARS-CoV-2, influenza A, and influenza B, which cause similar symptom complexes of flu cold in the respiratory tract. The detection method can be completed within 1 h, which is faster than other standard detection methods, and the limit of detection is approximately 10
MeSH term(s)	Humans ; Recombinases ; SARS-CoV-2 ; Influenza, Human ; CRISPR-Cas Systems ; COVID-19 ; Nucleotidyltransferases ; Orthomyxoviridae ; Nucleic Acid Amplification Techniques
Chemical Substances	Recombinases ; Nucleotidyltransferases (EC 2.7.7.-)
Language	English
Publishing date	2023-11-07
Publishing country	United States
Document type	Journal Article
ZDB-ID	752392-0
ISSN	1096-9071 ; 0146-6615
ISSN (online)	1096-9071
ISSN	0146-6615
DOI	10.1002/jmv.29215
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Article ; Online: Diagnostic value and characteristic analysis of serum nucleocapsid antigen in COVID-19 patients.

Zhang, Xihong / Qian, Chungen / Yang, Li / Gao, Huixia / Jiang, Ping / Dai, Muwei / Wang, Yuling / Kang, Haiyan / Xu, Yi / Hu, Qian / Feng, Fumin / Cheng, Bangning / Dai, Erhei

PeerJ

2023 Volume 11, Page(s) e15515

Abstract: Background: To date, several types of laboratory tests for coronavirus disease 2019 (COVID-19) diagnosis have been developed. However, the clinical importance of serum severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleocapsid antigen (N- ... ...

Abstract	Background: To date, several types of laboratory tests for coronavirus disease 2019 (COVID-19) diagnosis have been developed. However, the clinical importance of serum severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleocapsid antigen (N-Ag) remains to be fully elucidated. In this study, we sought to investigate the value of serum SARS-CoV-2 N-Ag for COVID-19 diagnosis and to analyze N-Ag characteristics in COVID-19 individuals. Methods: Serum samples collected from 215 COVID-19 patients and 65 non-COVID-19 individuals were used to quantitatively detect N-Ag Results: The sensitivity and specificity of the N-Ag assay were 64.75% (95% confidence interval (95% CI) [55.94-72.66%]) and 100% (95% CI [93.05-100.00%]), respectively, according to the cut-off value recommended by the manufacturer. The receiver operating characteristic (ROC) curve showed a sensitivity of 100.00% (95% CI [94.42-100.00%]) and a specificity of 71.31% (95% CI [62.73-78.59%]). The positive rates and levels of serum SARS-CoV-2 N-Ag were not related to sex, comorbidity status or disease severity of COVID-19 (all Conclusion: Serum N-Ag can be used as a biomarker for early COVID-19 diagnosis based on appropriate cut-off values. In addition, our study also demonstrated the relationship between serum N-Ag and clinical characteristics.
MeSH term(s)	Humans ; COVID-19/diagnosis ; COVID-19 Testing ; SARS-CoV-2 ; Nucleocapsid ; Antibodies, Neutralizing
Chemical Substances	Antibodies, Neutralizing
Language	English
Publishing date	2023-06-07
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2703241-3
ISSN	2167-8359 ; 2167-8359
ISSN (online)	2167-8359
ISSN	2167-8359
DOI	10.7717/peerj.15515
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