LIVIVO - Search results -

Search results

Result 1 - 10 of total 26

Search options

Article ; Online: Determination of sulfadoxine and pyrimethamine in microvolume human plasma using ultra high performance liquid chromatography-tandam mass spectrometry.

Sok, Vong / Marzan, Florence / Roh, Michelle / Guo, Kevin / Legac, Jenny / Mwebaza, Norah / Dorsey, Grant / Rosenthal, Philip J / Aweeka, Francesca T / Huang, Liusheng

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

2024 Volume 1234, Page(s) 124030

Abstract: To support the pharmacokinetic study of sulfadoxine (SD) and pyrimethamine (PM) in pregnant women and children, sensitive methods with small sample volume are desirable. Here we report a method to determine SD and PM with microvolume plasma samples: 5 µL ...

Abstract	To support the pharmacokinetic study of sulfadoxine (SD) and pyrimethamine (PM) in pregnant women and children, sensitive methods with small sample volume are desirable. Here we report a method to determine SD and PM with microvolume plasma samples: 5 µL plasma samples were cleaned up by protein precipitation with acetonitrile. The deuterated analytes were used as the internal standards. The samples after cleanup were injected onto an ACE Excel SuperC
MeSH term(s)	Pregnancy ; Child ; Humans ; Female ; Chromatography, High Pressure Liquid/methods ; Tandem Mass Spectrometry/methods ; Pyrimethamine ; Sulfadoxine ; Acetonitriles
Chemical Substances	Pyrimethamine (Z3614QOX8W) ; Sulfadoxine (88463U4SM5) ; Acetonitriles
Language	English
Publishing date	2024-01-24
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1180823-8
ISSN	1873-376X ; 0378-4347 ; 1570-0232 ; 1387-2273
ISSN (online)	1873-376X
ISSN	0378-4347 ; 1570-0232 ; 1387-2273
DOI	10.1016/j.jchromb.2024.124030
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 813: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Development and validation of an LC-MS/MS method for determination of hydroxychloroquine, its two metabolites, and azithromycin in EDTA-treated human plasma.

Sok, Vong / Marzan, Florence / Gingrich, David / Aweeka, Francesca / Huang, Liusheng

PloS one

2021 Volume 16, Issue 3, Page(s) e0247356

Abstract: Background: Hydroxychloroquine (HCQ) and azithromycin (AZM) are antimalarial drugs recently reported to be active against severe acute respiratory syndrome coronavirus- 2 (SARS-CoV-2), which is causing the global COVID-19 pandemic. In an emergency ... ...

Abstract	Background: Hydroxychloroquine (HCQ) and azithromycin (AZM) are antimalarial drugs recently reported to be active against severe acute respiratory syndrome coronavirus- 2 (SARS-CoV-2), which is causing the global COVID-19 pandemic. In an emergency response to the pandemic, we aimed to develop a quantitation method for HCQ, its metabolites desethylhydroxychloroquine (DHCQ) and bisdesethylchloroquine (BDCQ), and AZM in human plasma. Methods: Liquid chromatography tandem mass spectrometry was used to develop the method. Samples (20 μL) are extracted by solid-phase extraction and injected onto the LC-MS/MS system equipped with a PFP column (2.0 × 50 mm, 3 μm). ESI+ and MRM are used for detection. Ion pairs m/z 336.1→247.1 for HCQ, 308.1→179.1 for DHCQ, 264.1→179.1 for BDCQ, and 749.6→591.6 for AZM are selected for quantification. The ion pairs m/z 342.1→253.1, 314.1→181.1, 270.1→181.1, and 754.6→596.6 are selected for the corresponding deuterated internal standards (IS) HCQ-d4, DHCQ-d4, BDCQ-d4, and AZM-d5. The less abundant IS ions from 37Cl were used to overcome the interference from the analytes. Results: Under optimized conditions, retention times are 0.78 min for BDCQ, 0.79 min for DHCQ, 0.92 min for HCQ and 1.87 min for AZM. Total run time is 3.5 min per sample. The calibration ranges are 2-1000 ng/mL for HCQ and AZM, 1-500 ng/mL for DHCQ and 0.5-250 ng/mL for BDCQ; samples above the range are validated for up to 10-fold dilution. Recoveries of the method ranged from 88.9-94.4% for HCQ, 88.6-92.9% for DHCQ, 88.7-90.9% for BDCQ, and 98.6%-102% for AZM. The IS normalized matrix effect were within (100±10) % for all 4 analytes. Blood samples are stable for at least 6 hr at room temperature. Plasma samples are stable for at least 66 hr at room temperature, 38 days at -70°C, and 4 freeze-thaw cycles. Conclusions: An LC-MS/MS method for simultaneous quantitation of HCQ, DHCQ, BDCQ, and AZM in human plasma was developed and validated for clinical studies requiring fast turnaround time and small samples volume.
MeSH term(s)	Anti-Bacterial Agents/blood ; Antimalarials/blood ; Azithromycin/blood ; Blood Specimen Collection/methods ; Chloroquine/analogs & derivatives ; Chloroquine/blood ; Chromatography, High Pressure Liquid/methods ; Drug Monitoring/methods ; Edetic Acid/blood ; Humans ; Hydroxychloroquine/analogs & derivatives ; Hydroxychloroquine/blood ; Limit of Detection ; Tandem Mass Spectrometry/methods
Chemical Substances	Anti-Bacterial Agents ; Antimalarials ; Hydroxychloroquine (4QWG6N8QKH) ; Azithromycin (83905-01-5) ; cletoquine (83CVD213TU) ; Chloroquine (886U3H6UFF) ; Edetic Acid (9G34HU7RV0) ; N,N-dideethylchloroquine (M48JF33RF8)
Language	English
Publishing date	2021-03-05
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Validation Study
ISSN	1932-6203
ISSN (online)	1932-6203
DOI	10.1371/journal.pone.0247356
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Development and validation of an LC-MS/MS method for determination of hydroxychloroquine, its two metabolites, and azithromycin in EDTA-treated human plasma.

Vong Sok / Florence Marzan / David Gingrich / Francesca Aweeka / Liusheng Huang

PLoS ONE, Vol 16, Iss 3, p e

2021 Volume 0247356

Abstract: Background Hydroxychloroquine (HCQ) and azithromycin (AZM) are antimalarial drugs recently reported to be active against severe acute respiratory syndrome coronavirus- 2 (SARS-CoV-2), which is causing the global COVID-19 pandemic. In an emergency ... ...

Abstract	Background Hydroxychloroquine (HCQ) and azithromycin (AZM) are antimalarial drugs recently reported to be active against severe acute respiratory syndrome coronavirus- 2 (SARS-CoV-2), which is causing the global COVID-19 pandemic. In an emergency response to the pandemic, we aimed to develop a quantitation method for HCQ, its metabolites desethylhydroxychloroquine (DHCQ) and bisdesethylchloroquine (BDCQ), and AZM in human plasma. Methods Liquid chromatography tandem mass spectrometry was used to develop the method. Samples (20 μL) are extracted by solid-phase extraction and injected onto the LC-MS/MS system equipped with a PFP column (2.0 × 50 mm, 3 μm). ESI+ and MRM are used for detection. Ion pairs m/z 336.1→247.1 for HCQ, 308.1→179.1 for DHCQ, 264.1→179.1 for BDCQ, and 749.6→591.6 for AZM are selected for quantification. The ion pairs m/z 342.1→253.1, 314.1→181.1, 270.1→181.1, and 754.6→596.6 are selected for the corresponding deuterated internal standards (IS) HCQ-d4, DHCQ-d4, BDCQ-d4, and AZM-d5. The less abundant IS ions from 37Cl were used to overcome the interference from the analytes. Results Under optimized conditions, retention times are 0.78 min for BDCQ, 0.79 min for DHCQ, 0.92 min for HCQ and 1.87 min for AZM. Total run time is 3.5 min per sample. The calibration ranges are 2-1000 ng/mL for HCQ and AZM, 1-500 ng/mL for DHCQ and 0.5-250 ng/mL for BDCQ; samples above the range are validated for up to 10-fold dilution. Recoveries of the method ranged from 88.9-94.4% for HCQ, 88.6-92.9% for DHCQ, 88.7-90.9% for BDCQ, and 98.6%-102% for AZM. The IS normalized matrix effect were within (100±10) % for all 4 analytes. Blood samples are stable for at least 6 hr at room temperature. Plasma samples are stable for at least 66 hr at room temperature, 38 days at -70°C, and 4 freeze-thaw cycles. Conclusions An LC-MS/MS method for simultaneous quantitation of HCQ, DHCQ, BDCQ, and AZM in human plasma was developed and validated for clinical studies requiring fast turnaround time and small samples volume.
Keywords	Medicine ; R ; Science ; Q
Subject code	600
Language	English
Publishing date	2021-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Determination of unbound piperaquine in human plasma by ultra-high performance liquid chromatography tandem mass spectrometry.

Huang, Liusheng / Sok, Vong / Aslam-Mir, Usman / Marzan, Florence / Whalen, Meghan / Rosenthal, Philip J / Aweeka, Francesca

Journal of chromatography open

2022 Volume 2

Abstract: Piperaquine (PQ) is an antimalarial drug that is highly protein-bound. Variation in plasma protein contents may affect the pharmacokinetic (PK) exposure of unbound drug, leading to alteration of clinical outcomes. All published methods for determination ... ...

Abstract	Piperaquine (PQ) is an antimalarial drug that is highly protein-bound. Variation in plasma protein contents may affect the pharmacokinetic (PK) exposure of unbound drug, leading to alteration of clinical outcomes. All published methods for determination of PQ in human plasma measure the total PQ including both bound and unbound PQ to plasma proteins. There is no published method for unbound PQ determination. Here we report an ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method for determination of PQ in human plasma filtrate prepared by filtering human plasma through Millipore Microcon® centrifugal filters (10k NMWL). The filter cup had to be treated with 5% benzalkonium chloride to reduce non-specific binding to the filter devices before filtration of plasma samples. Multiple reactions monitoring (MRM) of the ion pairs
Language	English
Publishing date	2022-03-14
Publishing country	Netherlands
Document type	Journal Article
ISSN	2772-3917
ISSN (online)	2772-3917
DOI	10.1016/j.jcoa.2022.100042
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Determination of unbound piperaquine in human plasma by ultra-high performance liquid chromatography tandem mass spectrometry

Liusheng Huang / Vong Sok / Usman Aslam-Mir / Florence Marzan / Meghan Whalen / Philip J. Rosenthal / Francesca Aweeka

Journal of Chromatography Open, Vol 2, Iss , Pp 100042- (2022)

2022

Abstract	Piperaquine (PQ) is an antimalarial drug that is highly protein-bound. Variation in plasma protein contents may affect the pharmacokinetic (PK) exposure of unbound drug, leading to alteration of clinical outcomes. All published methods for determination of PQ in human plasma measure the total PQ including both bound and unbound PQ to plasma proteins. There is no published method for unbound PQ determination. Here we report an ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method for determination of PQ in human plasma filtrate prepared by filtering human plasma through Millipore Microcon® centrifugal filters (10k NMWL). The filter cup had to be treated with 5% benzalkonium chloride to reduce non-specific binding to the filter devices before filtration of plasma samples. Multiple reactions monitoring (MRM) of the ion pairs m/z 535/288 for PQ and m/z 541/294 for the internal standard (IS) was selected for quantification. When electrospray ionization (ESI+) was used, paradoxical matrix effect was observed despite the structure similarity of the deuterated IS: Ion suppression for PQ versus ion enhancement for the PQ-d6, even though they were closely eluted: 0.62 min versus 0.61 min. Separation was achieved on Evo C18 column (50 × 2.1 mm, 1.7 μm, Phenomenex Inc.) eluted with 10 mM NH4OH and MeCN. When atmospheric pressure chemical ionization in positive mode (APCI+) was used for ion source, matrix effect diminished. Separation was achieved on a PFP column (30 × 2.1 mm, 1.7 μm, Waters, Corp.) eluted with aqueous 20 mM ammonium formate 0.14% trifluoroacetic acid (A) and methanol-acetonitrile (4:1, v/v) containing 0.1% trifluoroacetic acid (B) at 0.8 mL/min flow rate in a gradient mode: 30–30–80–80–30–30%B (0–0.1–1.0–1.40–1.41–1.50 min). The retention time was 0.67 min for both PQ and the IS. The method was validated with a linear calibration range from 20 to 5,000 pg/mL and applied to clinical samples.
Keywords	Piperaquine ; UHPLC-MS/MS ; Plasma filtrate ; Plasma ; Unbound ; free ; Analytical chemistry ; QD71-142
Subject code	540
Language	English
Publishing date	2022-11-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Environmental contamination and risk factors for transmission of highly pathogenic avian influenza A(H5N1) to humans, Cambodia, 2006-2010.

Ly, Sowath / Vong, Sirenda / Cavailler, Philippe / Mumford, Elizabeth / Mey, Channa / Rith, Sareth / Van Kerkhove, Maria D / Sorn, San / Sok, Touch / Tarantola, Arnaud / Buchy, Philippe

BMC infectious diseases

2016 Volume 16, Issue 1, Page(s) 631

Abstract: Background: Highly pathogenic avian influenza A (H5N1) virus has been of public health concern since 2003. Probable risk factors for A(H5N1) transmission to human have been demonstrated in several studies or epidemiological reports. However, ... ...

Abstract	Background: Highly pathogenic avian influenza A (H5N1) virus has been of public health concern since 2003. Probable risk factors for A(H5N1) transmission to human have been demonstrated in several studies or epidemiological reports. However, transmission patterns may differ according to demographic characteristics of the population and local practices. This article aggregates these data from three studies with data collected in the previous surveys in 2006 and 2007 to further examine the risks factors associated with presence of anti-A(H5) antibodies among villagers residing within outbreak areas. Methods: We aggregated 5-year data (2006-2010) from serology survey and matched case-control studies in Cambodia to further examine the risks factors associated with A(H5N1) infection among villagers in the outbreak areas. Results: Serotesting among villagers detected 35 (1.5 % [0-2.6]) positive cases suggesting recent exposure to A(H5N1) virus. Practices associated with A(H5N1) infection among all ages were: having poultry cage or nesting area under or adjacent to the house (OR: 6.7 [1.6-28.3]; p = 0.010) and transporting poultry to market (OR: 17.6 [1.6-193.7]; p = 0.019). Practices found as risk factors for the infection among age under 20 years were swimming/bathing in ponds also accessed by domestic poultry (OR: 4.6 [1.1-19.1]; p = 0.038). Association with consuming wild birds reached borderline significance (p = 0.066). Conclusion: Our results suggest that swimming/bathing in contaminated pond water and close contact with poultry may present a risk of A(H5N1) transmission to human.
MeSH term(s)	Adolescent ; Adult ; Aged ; Aged, 80 and over ; Animals ; Animals, Wild/virology ; Cambodia/epidemiology ; Child ; Child, Preschool ; Disease Outbreaks/prevention & control ; Disease Outbreaks/statistics & numerical data ; Environmental Pollution ; Female ; Humans ; Infant ; Influenza A Virus, H5N1 Subtype/pathogenicity ; Influenza in Birds/transmission ; Influenza in Birds/virology ; Influenza, Human/transmission ; Influenza, Human/virology ; Male ; Middle Aged ; Ponds/virology ; Poultry/virology ; Public Health ; Risk Factors ; Water Pollutants ; Waterborne Diseases/transmission ; Waterborne Diseases/virology ; Young Adult
Chemical Substances	Water Pollutants
Language	English
Publishing date	2016-11-04
Publishing country	England
Document type	Journal Article
ZDB-ID	2041550-3
ISSN	1471-2334 ; 1471-2334
ISSN (online)	1471-2334
ISSN	1471-2334
DOI	10.1186/s12879-016-1950-z
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Going Out on a Limb: Delineating The Effects of β-Branching, N-Methylation, and Side Chain Size on the Passive Permeability, Solubility, and Flexibility of Sanguinamide A Analogues.

Bockus, Andrew T / Schwochert, Joshua A / Pye, Cameron R / Townsend, Chad E / Sok, Vong / Bednarek, Maria A / Lokey, R Scott

Journal of medicinal chemistry

2015 Volume 58, Issue 18, Page(s) 7409–7418

Abstract: It is well established that intramolecular hydrogen bonding and N-methylation play important roles in the passive permeability of cyclic peptides, but other structural features have been explored less intensively. Recent studies on the oral ... ...

Abstract	It is well established that intramolecular hydrogen bonding and N-methylation play important roles in the passive permeability of cyclic peptides, but other structural features have been explored less intensively. Recent studies on the oral bioavailability of the cyclic heptapeptide sanguinamide A have raised the question of whether steric occlusion of polar groups via β-branching is an effective, yet untapped, tool in cyclic peptide permeability optimization. We report the structures of 17 sanguinamide A analogues designed to test the relative contributions of β-branching, N-methylation, and side chain size to passive membrane permeability and aqueous solubility. We demonstrate that β-branching has little effect on permeability compared to the effects of aliphatic carbon count and N-methylation of exposed NH groups. We highlight a new N-methylated analogue of sanguinamide A with a Leu substitution at position 2 that exhibits solvent-dependent flexibility and improved permeability over that of the natural product.
MeSH term(s)	Caco-2 Cells ; Humans ; Membranes, Artificial ; Methylation ; Molecular Dynamics Simulation ; Peptides, Cyclic/chemistry ; Peptides, Cyclic/metabolism ; Permeability ; Solubility ; Structure-Activity Relationship ; Thiazoles/chemistry ; Thiazoles/metabolism ; Water
Chemical Substances	Membranes, Artificial ; Peptides, Cyclic ; Thiazoles ; sanguinamide A ; Water (059QF0KO0R)
Language	English
Publishing date	2015-09-24
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	218133-2
ISSN	1520-4804 ; 0022-2623
ISSN (online)	1520-4804
ISSN	0022-2623
DOI	10.1021/acs.jmedchem.5b00919
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.B 151: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular ab Jg. 2022: Lesesaal (EG)
Zs.MB 1: Show issues

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Cluster randomised trial of the impact of biosecurity measures on poultry health in backyard flocks.

Conan, Anne / Goutard, Flavie Luce / Holl, Davun / Ra, Sok / Ponsich, Aurélia / Tarantola, Arnaud / Sorn, San / Vong, Sirenda

Veterinary journal (London, England : 1997)

2013 Volume 198, Issue 3, Page(s) 649–655

Abstract: In Cambodia, most poultry are raised in backyard flocks with a low level of biosecurity, which increases the risk of spread of infectious diseases. The aim of this study was to evaluate the effectiveness of a practical biosecurity intervention based on ... ...

Abstract	In Cambodia, most poultry are raised in backyard flocks with a low level of biosecurity, which increases the risk of spread of infectious diseases. The aim of this study was to evaluate the effectiveness of a practical biosecurity intervention based on affordable basic measures. A cluster randomised trial was conducted in 18 villages in Cambodia from November 2009 to February 2011. Generalised estimating equations were used to test the association between the intervention and mortality rates in flocks of chickens and ducks. Mortality rates in chicken flocks in intervention villages (mean 6.3%, range 3.5-13.8%, per month) were significantly higher than in control villages (mean 4.5%, range 2.0-9.7%, per month; P<0.01). Mortality rates in duck flocks in intervention villages (mean 4.1%, range 1.9-7.9%, per month) were significantly higher than in control villages (mean 2.8%, range 0.6-8.0%, per month; P<0.01). Despite good compliance among poultry owners, the biosecurity intervention implemented in this study was not associated with improvements in poultry mortality rates. These findings suggest that basic biosecurity measures may not suffice to limit the spread of infectious diseases in backyard poultry flocks in Cambodia.
MeSH term(s)	Animal Husbandry/methods ; Animals ; Cambodia/epidemiology ; Chickens ; Communicable Disease Control/methods ; Ducks ; Influenza A Virus, H5N1 Subtype/isolation & purification ; Influenza in Birds/epidemiology ; Influenza in Birds/prevention & control ; Influenza in Birds/transmission ; Influenza in Birds/virology ; Newcastle Disease/epidemiology ; Newcastle Disease/prevention & control ; Newcastle Disease/transmission ; Newcastle Disease/virology ; Newcastle disease virus/isolation & purification ; Poultry Diseases/epidemiology ; Poultry Diseases/prevention & control ; Poultry Diseases/transmission ; Poultry Diseases/virology ; Random Allocation ; Seroepidemiologic Studies
Language	English
Publishing date	2013-12
Publishing country	England
Document type	Evaluation Studies ; Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	428614-5
ISSN	1532-2971 ; 0372-5545 ; 1090-0233
ISSN (online)	1532-2971
ISSN	0372-5545 ; 1090-0233
DOI	10.1016/j.tvjl.2013.09.010
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.B 552: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Serotype Distribution of Clinical

Inghammar, Malin / By, Youlet / Farris, Christina / Phe, Thong / Borand, Laurence / Kerleguer, Alexandra / Goyet, Sophie / Saphonn, Vonthanak / Phoeung, Chanleakhena / Vong, Sirenda / Rammaert, Blandine / Mayaud, Charles / Guillard, Bertrand / Yasuda, Chadwick / Kasper, Matthew R / Ford, Gavin / Newell, Steven W / An, Ung Sam / Sokhal, Buth /

Touch, Sok / Turner, Paul / Jacobs, Jan / Messaoudi, Mélina / Komurian-Pradel, Florence / Tarantola, Arnaud

The American journal of tropical medicine and hygiene

2018 Volume 98, Issue 3, Page(s) 791–796

Abstract: Childhood vaccination with the 13-valent pneumococcal conjugate vaccine (PCV13) was introduced in Cambodia in January 2015. Baseline data regarding circulating serotypes are scarce. All microbiology laboratories in Cambodia were contacted for ... ...

Abstract	Childhood vaccination with the 13-valent pneumococcal conjugate vaccine (PCV13) was introduced in Cambodia in January 2015. Baseline data regarding circulating serotypes are scarce. All microbiology laboratories in Cambodia were contacted for identification of stored isolates of
MeSH term(s)	Adolescent ; Adult ; Aged ; Aged, 80 and over ; Anti-Bacterial Agents/pharmacology ; Bacterial Typing Techniques ; Bronchoalveolar Lavage Fluid/microbiology ; Cambodia/epidemiology ; Child ; Child, Preschool ; Drug Resistance, Multiple, Bacterial ; Female ; Humans ; Infant ; Laboratories, Hospital ; Male ; Mass Vaccination ; Middle Aged ; Pneumococcal Vaccines ; Pneumonia, Pneumococcal/epidemiology ; Pneumonia, Pneumococcal/immunology ; Pneumonia, Pneumococcal/microbiology ; Pneumonia, Pneumococcal/prevention & control ; Serogroup ; Sputum/microbiology ; Streptococcus pneumoniae/classification ; Streptococcus pneumoniae/drug effects ; Streptococcus pneumoniae/immunology ; Streptococcus pneumoniae/isolation & purification ; Vaccines, Conjugate
Chemical Substances	13-valent pneumococcal vaccine ; Anti-Bacterial Agents ; Pneumococcal Vaccines ; Vaccines, Conjugate
Language	English
Publishing date	2018-01-04
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2942-7
ISSN	1476-1645 ; 0002-9637
ISSN (online)	1476-1645
ISSN	0002-9637
DOI	10.4269/ajtmh.17-0692
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Ud II Zs.61: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Cluster randomised trial of the impact of biosecurity measures on poultry health in backyard flocks

Conan, Anne / Goutard, Flavie Luce / Holl, Davun / Ra, Sok / Ponsich, Aurélia / Tarantola, Arnaud / Sorn, San / Vong, Sirenda

The Veterinary Journal. 2013 Dec., v. 198, no. 3

2013

Abstract	In Cambodia, most poultry are raised in backyard flocks with a low level of biosecurity, which increases the risk of spread of infectious diseases. The aim of this study was to evaluate the effectiveness of a practical biosecurity intervention based on affordable basic measures. A cluster randomised trial was conducted in 18 villages in Cambodia from November 2009 to February 2011. Generalised estimating equations were used to test the association between the intervention and mortality rates in flocks of chickens and ducks. Mortality rates in chicken flocks in intervention villages (mean 6.3%, range 3.5–13.8%, per month) were significantly higher than in control villages (mean 4.5%, range 2.0–9.7%, per month; P<0.01). Mortality rates in duck flocks in intervention villages (mean 4.1%, range 1.9–7.9%, per month) were significantly higher than in control villages (mean 2.8%, range 0.6–8.0%, per month; P<0.01). Despite good compliance among poultry owners, the biosecurity intervention implemented in this study was not associated with improvements in poultry mortality rates. These findings suggest that basic biosecurity measures may not suffice to limit the spread of infectious diseases in backyard poultry flocks in Cambodia.
Keywords	biosecurity ; chickens ; compliance ; ducks ; equations ; flocks ; infectious diseases ; mortality ; risk ; villages ; Cambodia
Language	English
Dates of publication	2013-12
Size	p. 649-655.
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	428614-5
ISSN	1532-2971 ; 0372-5545 ; 1090-0233
ISSN (online)	1532-2971
ISSN	0372-5545 ; 1090-0233
DOI	10.1016/j.tvjl.2013.09.010
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Bonn / Germany

Z 3664: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- See ZB MED holdings
- Order with fees

To top

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED