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Article ; Online: Determination of Kinetic Parameters of Fischer–Tropsch Synthesis in the Presence of a Granular Cobalt Catalyst

Lilia Sineva / Kirill Gryaznov / Victor De / Andrei Gorshkov / Vladimir Mordkovich

Reactions, Vol 4, Iss 4, Pp 92-

2023 Volume 105

Abstract: Some kinetic parameters of Fischer–Tropsch synthesis (FTS) were determined in the presence of a granular cobalt/zeolite catalyst. Usually, kinetic studies of granular catalysts are considered to be complicated by external and internal diffusion. We ... ...

Abstract	Some kinetic parameters of Fischer–Tropsch synthesis (FTS) were determined in the presence of a granular cobalt/zeolite catalyst. Usually, kinetic studies of granular catalysts are considered to be complicated by external and internal diffusion. We managed to obtain a catalyst with a special structure of the active surface, where sites active in FTS are isolated from each other due to the environment of inactive spinel CoAl 2 O 4 and inter-site transport is provided by an extensive intragranular graphitic network serving simultaneously as a heat-conductive medium. As a result, FTS proceeded in the kinetic region. It was found that the reaction kinetics obey the Arrhenius law; whereas, the activation energy is different in different temperature ranges, i.e., 118.2 kJ/mol in the range of 180–210 °C, and 173.6 kJ/mol in the range of 232–243 °C. This behavior is determined by the presence of zeolite, which becomes active in the secondary transformations of FTS products at temperatures beyond 210 °C.
Keywords	Fischer–Tropsch synthesis ; granulated catalyst ; heterogeneous catalysis ; activation energy ; order of reaction ; Chemistry ; QD1-999
Subject code	660
Language	English
Publishing date	2023-01-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: How the immune mousetrap works: Structural evidence for the immunomodulatory action of a peptide from influenza NS1 protein.

Zabrodskaya, Yana / Tsvetkov, Vladimir / Shurygina, Anna-Polina / Vasyliev, Kirill / Shaldzhyan, Aram / Gorshkov, Andrey / Kuklin, Alexander / Fedorova, Natalya / Egorov, Vladimir

Biophysical chemistry

2024 Volume 307, Page(s) 107176

Abstract: One of the critical stages of the T-cell immune response is the dimerization of the intramembrane domains of T-cell receptors (TCR). Structural similarities between the immunosuppressive domains of viral proteins and the transmembrane domains of TCR have ...

Abstract	One of the critical stages of the T-cell immune response is the dimerization of the intramembrane domains of T-cell receptors (TCR). Structural similarities between the immunosuppressive domains of viral proteins and the transmembrane domains of TCR have led several authors to hypothesize the mechanism of immune response suppression by highly pathogenic viruses: viral proteins embed themselves in the membrane and act on the intramembrane domain of the TCRalpha subunit, hindering its functional oligomerization. It has also been suggested that this mechanism is used by influenza A virus in NS1-mediated immunosuppression. We have shown that the peptide corresponding to the primary structure of the potential immunosuppressive domain of NS1 protein (G51) can reduce concanavalin A-induced proliferation of PBMC cells, as well as in vitro, G51 can affect the oligomerization of the core peptide corresponding to the intramembrane domain of TCR, using AFM and small-angle neutron scattering. The results obtained using in cellulo and in vitro model systems suggest the presence of functional interaction between the NS1 fragment and the intramembrane domain of the TCR alpha subunit. We have proposed a possible scheme for such interaction obtained by computer modeling. This suggests the existence of another NS1-mediated mechanism of immunosuppression in influenza.
MeSH term(s)	Humans ; Influenza, Human ; Leukocytes, Mononuclear/metabolism ; Peptides/pharmacology ; Immunity ; Viral Proteins ; Receptors, Antigen, T-Cell ; Viral Nonstructural Proteins/chemistry
Chemical Substances	Peptides ; Viral Proteins ; Receptors, Antigen, T-Cell ; Viral Nonstructural Proteins
Language	English
Publishing date	2024-01-09
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	185052-0
ISSN	1873-4200 ; 0301-4622
ISSN (online)	1873-4200
ISSN	0301-4622
DOI	10.1016/j.bpc.2024.107176
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Article ; Online: Fluorescent quantum dots enable SARS-CoV-2 antiviral drug discovery and development.

Gorshkov, Kirill / Susumu, Kimihiro / Wolak, Mason / Oh, Eunkeu

Expert opinion on drug discovery

2021 Volume 17, Issue 3, Page(s) 225–230

Abstract: Introduction: SARS-CoV-2 is a highly infectious and deadly coronavirus whose study requires the use of a biosafety level 3 (BSL-3) containment facility to investigate viral biology and pathogenesis, which limits the study of live virus and slows ... ...

Abstract	Introduction: SARS-CoV-2 is a highly infectious and deadly coronavirus whose study requires the use of a biosafety level 3 (BSL-3) containment facility to investigate viral biology and pathogenesis, which limits the study of live virus and slows progress toward finding suitable treatments for infection. While vaccines from several companies have proven very effective in combating the virus, few treatments exist for those who do succumb to the viral-induced systemic disease called COVID-19. Areas covered: This short review focuses on fluorescent quantum dot-based modeling of SARS-CoV-2. New BSL-2 viral models are essential for finding small molecules and biologics that may be effective in stopping viral infection, as well as treating already infected individuals. Nanoparticles are invaluable tools for biological research as they can be used to both model pathogens and serve as a platform for developing vaccines. Expert opinion: Visualizing viral activity with fluorescent quantum dots enables both biochemical and cell-based assays to detect virus-host receptor interactions, cellular activity after binding to the cell plasma membrane, screening for interventions using small-molecule drug repurposing, and testing of novel biologics. Quantum dots can also be used for diagnostic assays, vaccine development, and importantly, pan-antiviral drugs to address variants that may escape the immune response.
MeSH term(s)	Antiviral Agents/pharmacology ; COVID-19/drug therapy ; Drug Discovery ; Humans ; Quantum Dots ; SARS-CoV-2
Chemical Substances	Antiviral Agents
Language	English
Publishing date	2021-12-02
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Intramural
ZDB-ID	2259618-5
ISSN	1746-045X ; 1746-0441
ISSN (online)	1746-045X
ISSN	1746-0441
DOI	10.1080/17460441.2022.2005025
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Article ; Online: High-throughput Confocal Imaging of Quantum Dot-Conjugated SARS-CoV-2 Spike Trimers to Track Binding and Endocytosis in HEK293T Cells.

Tran, Bruce Nguyen / Oh, Eunkeu / Susumu, Kimihiro / Wolak, Mason / Gorshkov, Kirill

Journal of visualized experiments : JoVE

2022 , Issue 182

Abstract: The development of new technologies for cellular fluorescence microscopy has facilitated high-throughput screening methods for drug discovery. Quantum dots are fluorescent nanoparticles with excellent photophysical properties imbued with bright and ... ...

Abstract	The development of new technologies for cellular fluorescence microscopy has facilitated high-throughput screening methods for drug discovery. Quantum dots are fluorescent nanoparticles with excellent photophysical properties imbued with bright and stable photoluminescence as well as narrow emission bands. Quantum dots are spherical in shape, and with the proper modification of the surface chemistry, can be used to conjugate biomolecules for cellular applications. These optical properties, combined with the ability to functionalize them with biomolecules, make them an excellent tool for investigating receptor-ligand interactions and cellular trafficking. Here, we present a method that uses quantum dots to track the binding and endocytosis of SARS-CoV-2 spike protein. This protocol can be used as a guide for experimentalists looking to utilize quantum dots to study protein-protein interactions and trafficking in the context of cellular physiology.
MeSH term(s)	Endocytosis ; HEK293 Cells ; Humans ; Quantum Dots ; SARS-CoV-2 ; Spike Glycoprotein, Coronavirus/analysis
Chemical Substances	Spike Glycoprotein, Coronavirus ; spike protein, SARS-CoV-2
Language	English
Publishing date	2022-04-21
Publishing country	United States
Document type	Journal Article ; Video-Audio Media
ZDB-ID	2259946-0
ISSN	1940-087X ; 1940-087X
ISSN (online)	1940-087X
ISSN	1940-087X
DOI	10.3791/63202
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Article ; Online: Are circadian rhythms in disarray in patients with chronic critical illness?

Kanarskii, Mikhail / Nekrasova, Julia / Kondratieva, Ekaterina / Borisov, Ilya / Simenel, Elena / Sviryaev, Yurii / Pradhan, Pranil / Gorshkov, Kirill / Shestopalov, Alexander / Petrova, Marina

Sleep medicine: X

2023 Volume 7, Page(s) 100101

Abstract: Aim: The aim of our study is to assess circadian rhythms in patients with chronic critical illness due to severe brain injury in intensive care unit by establishing the relation between melatonin and cortisol secretion, considering astronomical time and ...

Abstract	Aim: The aim of our study is to assess circadian rhythms in patients with chronic critical illness due to severe brain injury in intensive care unit by establishing the relation between melatonin and cortisol secretion, considering astronomical time and the sleep-wake cycle in chronic critical illness. Materials and methods: The study included 54 adult patients with chronic critical illness who resided in the intensive care unit for at least 30 days. The level of consciousness was determined using the CRS-R scale. We did the continuous electroencephalographic (EEG) monitoring with polygraphic leads for 24 h. Also, we determined the serum levels of cortisol and melatonin using the tandem mass spectrometry method with ultra-performance liquid chromatography. Results: 90.74 % of patients had one acrophase in melatonin secretion curve, which suggests the preservation of the rhythmic secretion of melatonin. These acrophases of the melatonin rhythm occurred during the night time in 91.8 % of patients. Most of the patients (69.3 %) slept during the period from 2:00 to 4:00 a.m. The evening levels of cortisol and melatonin had an inverse relation ( Conclusions: We concluded from our study that the rhythmic secretion of melatonin and cortisol is preserved in patients with chronic critical illness that resulted from severe brain injury. No statistically significant discrepancy between melatonin and cortisol secretion, day-and-night time and the sleep-wake cycle are found. We may focus our future work on finding more reliable methods to stabilize the preservation of circadian rhythms to protect vital organ functions.
Language	English
Publishing date	2023-12-23
Publishing country	Netherlands
Document type	Journal Article
ISSN	2590-1427
ISSN (online)	2590-1427
DOI	10.1016/j.sleepx.2023.100101
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Article: High-throughput confocal imaging of quantum dot-conjugated sars-cov-2 spike trimers to track binding and endocytosis in hek293t cells

Tran, Bruce Nguyen / Oh, Eunkeu / Susumu, Kimihiro / Wolak, Mason / Gorshkov, Kirill

Journal of visualized experiments. 2022 Apr. 21, , no. 182

2022

Abstract	The development of new technologies for cellular fluorescence microscopy has facilitated high-throughput screening methods for drug discovery. Quantum dots are fluorescent nanoparticles with excellent photophysical properties imbued with bright and stable photoluminescence as well as narrow emission bands. Quantum dots are spherical in shape, and with the proper modification of the surface chemistry, can be used to conjugate biomolecules for cellular applications. These optical properties, combined with the ability to functionalize them with biomolecules, make them an excellent tool for investigating receptor-ligand interactions and cellular trafficking. Here, we present a method that uses quantum dots to track the binding and endocytosis of SARS-CoV-2 spike protein. This protocol can be used as a guide for experimentalists looking to utilize quantum dots to study protein-protein interactions and trafficking in the context of cellular physiology.
Keywords	Severe acute respiratory syndrome coronavirus 2 ; biochemical compounds ; drugs ; endocytosis ; fluorescence ; fluorescence microscopy ; ligands ; photoluminescence ; physiology
Language	English
Dates of publication	2022-0421
Size	p. e63202.
Publishing place	Journal of Visualized Experiments
Document type	Article
ZDB-ID	2259946-0
ISSN	1940-087X
ISSN	1940-087X
DOI	10.3791/63202
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Drug Discovery Strategies for SARS-CoV-2.

Shyr, Zeenat A / Gorshkov, Kirill / Chen, Catherine Z / Zheng, Wei

The Journal of pharmacology and experimental therapeutics

2020 Volume 375, Issue 1, Page(s) 127–138

Abstract: Coronavirus disease 2019 (COVID-19) is a novel disease caused by the severe acute respiratory syndrome coronavirus (SARS-CoV)-2 virus that was first detected in December of 2019 in Wuhan, China, and has rapidly spread worldwide. The search for a suitable ...

Abstract	Coronavirus disease 2019 (COVID-19) is a novel disease caused by the severe acute respiratory syndrome coronavirus (SARS-CoV)-2 virus that was first detected in December of 2019 in Wuhan, China, and has rapidly spread worldwide. The search for a suitable vaccine as well as effective therapeutics for the treatment of COVID-19 is underway. Drug repurposing screens provide a useful and effective solution for identifying potential therapeutics against SARS-CoV-2. For example, the experimental drug remdesivir, originally developed for Ebola virus infections, has been approved by the US Food and Drug Administration as an emergency use treatment of COVID-19. However, the efficacy and toxicity of this drug need further improvements. In this review, we discuss recent findings on the pathology of coronaviruses and the drug targets for the treatment of COVID-19. Both SARS-CoV-2-specific inhibitors and broad-spectrum anticoronavirus drugs against SARS-CoV, Middle East respiratory syndrome coronavirus, and SARS-CoV-2 will be valuable additions to the anti-SARS-CoV-2 armament. A multitarget treatment approach with synergistic drug combinations containing different mechanisms of action may be a practical therapeutic strategy for the treatment of severe COVID-19. SIGNIFICANCE STATEMENT: Understanding the biology and pathology of RNA viruses is critical to accomplish the challenging task of developing vaccines and therapeutics against SARS-CoV-2. This review highlights the anti-SARS-CoV-2 drug targets and therapeutic development strategies for COVID-19 treatment.
MeSH term(s)	Antiviral Agents/chemistry ; Antiviral Agents/pharmacology ; Antiviral Agents/therapeutic use ; Betacoronavirus/drug effects ; Betacoronavirus/immunology ; Betacoronavirus/physiology ; COVID-19 ; COVID-19 Vaccines ; Clinical Trials as Topic ; Coronavirus Infections/drug therapy ; Coronavirus Infections/immunology ; Coronavirus Infections/prevention & control ; Drug Discovery/methods ; Humans ; Pandemics/prevention & control ; Pneumonia, Viral/drug therapy ; Pneumonia, Viral/prevention & control ; SARS-CoV-2 ; Viral Vaccines/immunology ; Viral Vaccines/therapeutic use ; Virus Internalization/drug effects ; Virus Replication/drug effects
Chemical Substances	Antiviral Agents ; COVID-19 Vaccines ; Viral Vaccines
Keywords	covid19
Language	English
Publishing date	2020-07-28
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Intramural ; Review
ZDB-ID	3106-9
ISSN	1521-0103 ; 0022-3565
ISSN (online)	1521-0103
ISSN	0022-3565
DOI	10.1124/jpet.120.000123
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Article ; Online: Astrocytes as targets for drug discovery.

Gorshkov, Kirill / Aguisanda, Francis / Thorne, Natasha / Zheng, Wei

Drug discovery today

2018 Volume 23, Issue 3, Page(s) 673–680

Abstract: Recent studies have illuminated the crucial role of astrocytes in maintaining proper neuronal health and function. Abnormalities in astrocytic functions have now been implicated in the pathogenesis of neurodegenerative diseases, including Alzheimer's ... ...

Abstract	Recent studies have illuminated the crucial role of astrocytes in maintaining proper neuronal health and function. Abnormalities in astrocytic functions have now been implicated in the pathogenesis of neurodegenerative diseases, including Alzheimer's disease (AD), Parkinson's disease (PD), Huntington's disease (HD), and amyotrophic lateral sclerosis (ALS). Historically, drug development programs for neurodegenerative diseases generally target only neurons, overlooking the contributions of astrocytes. Therefore, targeting both disease neurons and astrocytes offers a new approach for drug development for the treatment of neurological diseases. Looking forward, the co-culturing of human neurons with astrocytes could be the next evolutionary step in drug discovery for neurodegenerative diseases.
MeSH term(s)	Animals ; Astrocytes/drug effects ; Drug Discovery/methods ; Humans ; Neurodegenerative Diseases/drug therapy ; Neurons/drug effects ; Pharmaceutical Preparations/administration & dosage
Chemical Substances	Pharmaceutical Preparations
Language	English
Publishing date	2018-01-06
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Intramural ; Review
ZDB-ID	1324988-5
ISSN	1878-5832 ; 1359-6446
ISSN (online)	1878-5832
ISSN	1359-6446
DOI	10.1016/j.drudis.2018.01.011
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Zs.A 4725: Show issues

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Article ; Online: Yeast Ribonucleotide Reductase Is a Direct Target of the Proteasome and Provides Hyper Resistance to the Carcinogen 4-NQO.

Spasskaya, Daria S / Kulagin, Kirill A / Grineva, Evgenia N / Osipova, Pamila J / Poddubko, Svetlana V / Bubis, Julia A / Kazakova, Elizaveta M / Kusainova, Tomiris T / Gorshkov, Vladimir A / Kjeldsen, Frank / Karpov, Vadim L / Tarasova, Irina A / Karpov, Dmitry S

Journal of fungi (Basel, Switzerland)

2023 Volume 9, Issue 3

Abstract: Various external and internal factors damaging DNA constantly disrupt the stability of the genome. Cells use numerous dedicated DNA repair systems to detect damage and restore genomic integrity in a timely manner. Ribonucleotide reductase (RNR) is a key ... ...

Abstract	Various external and internal factors damaging DNA constantly disrupt the stability of the genome. Cells use numerous dedicated DNA repair systems to detect damage and restore genomic integrity in a timely manner. Ribonucleotide reductase (RNR) is a key enzyme providing dNTPs for DNA repair. Molecular mechanisms of indirect regulation of yeast RNR activity are well understood, whereas little is known about its direct regulation. The study was aimed at elucidation of the proteasome-dependent mechanism of direct regulation of RNR subunits in
Language	English
Publishing date	2023-03-14
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2784229-0
ISSN	2309-608X ; 2309-608X
ISSN (online)	2309-608X
ISSN	2309-608X
DOI	10.3390/jof9030351
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Article ; Online: Activation of Anthracite Combustion Using Pyrolysis Oil from Thermal Conversion of Waste Car Tires.

Larionov, Kirill B / Slyusarskiy, Konstantin V / Tsibulskiy, Svyatoslav A / Kaltaev, Albert Zh / Berezikov, Nikolay I / Gorshkov, Alexander S / Lavrinenko, Sergey V / Gubin, Vladimir E

ACS omega

2021 Volume 6, Issue 30, Page(s) 19731–19739

Abstract: The ignition and combustion of anthracite modified by the addition of pyrolysis oil obtained during thermal processing of waste car tires (WCTs) had been studied. The mass fraction of WCT pyrolysis oil was varied in the range from 5 to 30 wt %. The ... ...

Abstract	The ignition and combustion of anthracite modified by the addition of pyrolysis oil obtained during thermal processing of waste car tires (WCTs) had been studied. The mass fraction of WCT pyrolysis oil was varied in the range from 5 to 30 wt %. The additive was applied by the drop impregnation method. Ignition and combustion of obtained samples were carried out in a combustion chamber at temperatures of the heating medium
Language	English
Publishing date	2021-07-21
Publishing country	United States
Document type	Journal Article
ISSN	2470-1343
ISSN (online)	2470-1343
DOI	10.1021/acsomega.1c02404
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