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  1. Article ; Online: Visualization and characterisation of microplastics in aquatic environment using a home-built micro-Raman spectroscopic set up.

    Sunil, Megha / N, Mithun / Charles, Meril / Chidangil, Santhosh / Kumar, Satheesh / Lukose, Jijo

    Journal of environmental management

    2024  Volume 354, Page(s) 120351

    Abstract: Microplastics (MP) which are tiny plastic particles of sizes range from 1 μm (μm) to 5 mm (mm), have become a growing cause of concern due to their widespread presence in the environment and their potential impacts on ecosystems and human health. Marine ... ...

    Abstract Microplastics (MP) which are tiny plastic particles of sizes range from 1 μm (μm) to 5 mm (mm), have become a growing cause of concern due to their widespread presence in the environment and their potential impacts on ecosystems and human health. Marine organisms have the potential to consume microplastics, which could lead to physical injuries, blockages, or the transfer of harmful substances up the food chain. Humans may indirectly consume microplastics through contaminated seafood and water, although the complete scope of health risks is currently under investigation. An essential step in gaining a comprehensive understanding of microplastic pollution in waterbodies is the identification of microplastics, which is also crucial for further development of effective environmental regulations to address its adverse impacts. Majority of the researchers are accomplishing it globally using commercial platforms based on Raman spectroscopy. However, the development of indigenous Raman systems, which can enable microplastic identification, particularly in developing nations, is the need of the hour due to the outrageous cost of commercial platforms. In the current study, a custom-designed micro-Raman spectroscopy system was developed to detect and characterize microplastics from waterbodies. The developed system enabled visualization, size measurement and characterization of microplastics. Experimental parameters were fine-tuned, and a standardized Raman database was established for each type of plastic. This system exhibited high resolution which was capable of analysing microparticles of size up to 5 μm. Principal component analysis was carried out on the experimental Raman data, demonstrating good classification amongst different kinds of plastics. The performance of the developed system in analysing real samples was evaluated through experiments conducted on water samples obtained from the shore of Malpe Beach in Udupi district. The results revealed the presence of polyethylene and polyethylene terephthalate in the samples, along with the detection of pigments like copper phthalocyanine and indigo blue.
    MeSH term(s) Humans ; Microplastics/analysis ; Plastics/chemistry ; Ecosystem ; Spectrum Analysis, Raman ; Water/analysis ; Water Pollutants, Chemical/analysis ; Environmental Monitoring
    Chemical Substances Microplastics ; Plastics ; Water (059QF0KO0R) ; Water Pollutants, Chemical
    Language English
    Publishing date 2024-02-20
    Publishing country England
    Document type Journal Article
    ZDB-ID 184882-3
    ISSN 1095-8630 ; 0301-4797
    ISSN (online) 1095-8630
    ISSN 0301-4797
    DOI 10.1016/j.jenvman.2024.120351
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Raman spectroscopy for viral diagnostics.

    Lukose, Jijo / Barik, Ajaya Kumar / Mithun N / Sanoop Pavithran M / George, Sajan D / Murukeshan, V M / Chidangil, Santhosh

    Biophysical reviews

    2023  Volume 15, Issue 2, Page(s) 199–221

    Language English
    Publishing date 2023-04-10
    Publishing country Germany
    Document type Journal Article ; Review
    ZDB-ID 2486483-3
    ISSN 1867-2469 ; 1867-2450
    ISSN (online) 1867-2469
    ISSN 1867-2450
    DOI 10.1007/s12551-023-01059-4
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Serum protein profile study of myocardial infarction using a LED induced fluorescence based HPLC system.

    John, Reena V / Devasia, Tom / Adigal, Sphurti S / Lukose, Jijo / Kartha, V B / Chidangil, Santhosh

    Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

    2023  Volume 1217, Page(s) 123616

    Abstract: Cardiovascular diseases (CVDs) are the major health conditions for high mortality and morbidity in humans. Delay in the diagnosis of CVDs effect patients long and short-term health condition. In -house assembled UV-light emitting diode (LED) based ... ...

    Abstract Cardiovascular diseases (CVDs) are the major health conditions for high mortality and morbidity in humans. Delay in the diagnosis of CVDs effect patients long and short-term health condition. In -house assembled UV-light emitting diode (LED) based fluorescence detector for high -performance liquid chromatography (HPLC) (HPLC-LED-IF) system is used to record serum chromatograms of three categories of samples namely, before medicated- myocardial infarction (B-MI), after medicated- MI (A-MI), and normal. The sensitivity and performance of HPLC-LED-IF system is estimated using commercial serum proteins. Statistical analysis tools like, descriptive statistics, principal component analysis (PCA), and Match/ No Match test were applied to visualize the variation in three groups of samples. Statistical analysis of the protein profile data showed fairly good discrimination among the three categories. The receiver operating characteristic (ROC) curve also supported the reliability of the method to diagnose MI.
    MeSH term(s) Humans ; Chromatography, High Pressure Liquid/methods ; Reproducibility of Results ; ROC Curve ; Myocardial Infarction
    Language English
    Publishing date 2023-02-10
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 1180823-8
    ISSN 1873-376X ; 0378-4347 ; 1570-0232 ; 1387-2273
    ISSN (online) 1873-376X
    ISSN 0378-4347 ; 1570-0232 ; 1387-2273
    DOI 10.1016/j.jchromb.2023.123616
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Optical technologies for the detection of viruses like COVID-19: Progress and prospects.

    Lukose, Jijo / Chidangil, Santhosh / George, Sajan D

    Biosensors & bioelectronics

    2021  Volume 178, Page(s) 113004

    Abstract: The outbreak of life-threatening pandemic like COVID-19 necessitated the development of novel, rapid and cost-effective techniques that facilitate detection of viruses like SARS-CoV-2. The presently popular approach of a collection of samples using the ... ...

    Abstract The outbreak of life-threatening pandemic like COVID-19 necessitated the development of novel, rapid and cost-effective techniques that facilitate detection of viruses like SARS-CoV-2. The presently popular approach of a collection of samples using the nasopharyngeal swab method and subsequent detection of RNA using the real-time polymerase chain reaction suffers from false-positive results and a longer diagnostic time scale. Alternatively, various optical techniques namely optical sensing, spectroscopy, and imaging shows a great promise in virus detection. Herein, a comprehensive review of the various photonics technologies employed for virus detection, particularly the SARS-CoV family, is discussed. The state-of-art research activities in utilizing the photonics tools such as near-infrared spectroscopy, Fourier transform infrared spectroscopy, Raman spectroscopy, fluorescence-based techniques, super-resolution microscopy, surface plasmon resonance-based detection, for virus detection accounted extensively with an emphasis on coronavirus detection. Further, an account of emerging photonics technologies of SARS-CoV-2 detection and future possibilities is also explained. The progress in the field of optical techniques for virus detection unambiguously show a great promise in the development of rapid photonics-based devices for COVID-19 detection.
    MeSH term(s) Biosensing Techniques/methods ; COVID-19/diagnosis ; COVID-19/virology ; COVID-19 Testing/methods ; COVID-19 Testing/trends ; Humans ; Molecular Diagnostic Techniques ; Nucleic Acid Amplification Techniques ; Optical Phenomena ; Pandemics ; SARS-CoV-2/genetics ; SARS-CoV-2/isolation & purification ; Spectroscopy, Fourier Transform Infrared ; Spectrum Analysis, Raman ; Surface Plasmon Resonance
    Language English
    Publishing date 2021-01-16
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 1011023-9
    ISSN 1873-4235 ; 0956-5663
    ISSN (online) 1873-4235
    ISSN 0956-5663
    DOI 10.1016/j.bios.2021.113004
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Probing Nanoparticle–Cell Interaction Using Micro-Raman Spectroscopy

    Surekha Barkur / Jijo Lukose / Santhosh Chidangil

    ACS Omega, Vol 5, Iss 3, Pp 1439-

    Silver and Gold Nanoparticle-Induced Stress Effects on Optically Trapped Live Red Blood Cells

    2020  Volume 1447

    Keywords Chemistry ; QD1-999
    Language English
    Publishing date 2020-01-01T00:00:00Z
    Publisher American Chemical Society
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: Micro-Raman spectroscopy study of blood samples from myocardial infarction patients.

    John, Reena V / Devasia, Tom / N, Mithun / Lukose, Jijo / Chidangil, Santhosh

    Lasers in medical science

    2022  Volume 37, Issue 9, Page(s) 3451–3460

    Abstract: Acute myocardial infarction (MI) is found to be a major causative factor for global mortality and morbidity. This situation demands necessity of developing efficient and rapid diagnostic tools to detect acute MI. Raman spectroscopy is a non-destructive ... ...

    Abstract Acute myocardial infarction (MI) is found to be a major causative factor for global mortality and morbidity. This situation demands necessity of developing efficient and rapid diagnostic tools to detect acute MI. Raman spectroscopy is a non-destructive optical diagnostic technique, which has high potential in probing biochemical changes in clinical samples during initiation and progress of diseases. In this work, blood was taken as the sample to examine inflammation in acute MI patients using Raman spectroscopy. Ratio of Raman peak intensities that corresponds to phenylalanine (1000 cm
    MeSH term(s) Humans ; Spectrum Analysis, Raman/methods ; Phenylalanine ; Tyrosine ; Myocardial Infarction/diagnosis ; Inflammation
    Chemical Substances Phenylalanine (47E5O17Y3R) ; Tyrosine (42HK56048U)
    Language English
    Publishing date 2022-07-12
    Publishing country England
    Document type Journal Article
    ZDB-ID 632808-8
    ISSN 1435-604X ; 0268-8921
    ISSN (online) 1435-604X
    ISSN 0268-8921
    DOI 10.1007/s10103-022-03604-1
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Single cell spectroscopy of red blood cells in intravenous crystalloid fluids.

    N, Mithun / Lukose, Jijo / Mohan, Ganesh / Shastry, Shamee / Chidangil, Santhosh

    Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy

    2021  Volume 257, Page(s) 119726

    Abstract: Crystalloid fluids, a subset of intravenous (IV) fluid solutions are commonly used in clinical settings. The influence of these fluids on the functions of blood components are least explored. Raman spectroscopy combined with optical trapping has been ... ...

    Abstract Crystalloid fluids, a subset of intravenous (IV) fluid solutions are commonly used in clinical settings. The influence of these fluids on the functions of blood components are least explored. Raman spectroscopy combined with optical trapping has been widely used to evaluate the impact of external stress agents on red blood cells. The present study investigates the impact of commonly used crystalloid fluids on red blood cells in comparison with that of blood plasma using Raman Tweezers spectroscopy. The red blood cells suspended in crystalloid fluids undergo deoxygenation readily than that in blood plasma. In addition, cells in blood plasma were able to withstand laser induced deoxygenation comparatively better than that in crystalloid fluids at higher laser powers. Principle component analysis of the Raman spectral data has clearly demonstrated the discrimination of cells in plasma with that of crystalloid fluids demonstrating the effect of external induced stress on RBCs.
    MeSH term(s) Crystalloid Solutions ; Erythrocytes ; Lasers ; Spectrum Analysis, Raman
    Chemical Substances Crystalloid Solutions
    Language English
    Publishing date 2021-03-19
    Publishing country England
    Document type Journal Article
    ZDB-ID 210413-1
    ISSN 1873-3557 ; 0370-8322 ; 0584-8539 ; 1386-1425
    ISSN (online) 1873-3557
    ISSN 0370-8322 ; 0584-8539 ; 1386-1425
    DOI 10.1016/j.saa.2021.119726
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Probing Nanoparticle-Cell Interaction Using Micro-Raman Spectroscopy: Silver and Gold Nanoparticle-Induced Stress Effects on Optically Trapped Live Red Blood Cells.

    Barkur, Surekha / Lukose, Jijo / Chidangil, Santhosh

    ACS omega

    2020  Volume 5, Issue 3, Page(s) 1439–1447

    Abstract: Advancements in the field of nanotechnology have resulted in the emergence of a large variety of engineered nanomaterials for innumerable applications. Despite the ubiquitous use of nanomaterials in daily life, concerns regarding the potential toxicity ... ...

    Abstract Advancements in the field of nanotechnology have resulted in the emergence of a large variety of engineered nanomaterials for innumerable applications. Despite the ubiquitous use of nanomaterials in daily life, concerns regarding the potential toxicity and safety of these materials have also been raised. There is a high demand for assessing the unwanted effects of both gold and silver nanoparticles, which is increasingly being used in biomedical applications. This paper deals with the study of stress due to silver and gold nanoparticles of varying size on red blood cells (RBCs) using Raman tweezers spectroscopy. RBCs were incubated with nanoparticles of size in the 10-100 nm range with the same concentrations, and micro-Raman spectra were recorded by optically trapping the nanoparticle-treated live RBCs. Spectral modifications implicating hemoglobin deoxygenation were observed in all nanoparticle-treated RBCs. One of the probable reason for the deoxygenation trend can be the adhesion of nanoparticles onto the cell surface causing imbalance in cell functioning. Moreover, the higher spectral variations observed for silver nanoparticles indicate that oxidative stress is involved in cell damage. These mechanisms lead to the modification in the hemoglobin structure because of changes in the pH of cytoplasm, which can be detected using Raman spectroscopy.
    Language English
    Publishing date 2020-01-10
    Publishing country United States
    Document type Journal Article
    ISSN 2470-1343
    ISSN (online) 2470-1343
    DOI 10.1021/acsomega.9b02988
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Laser induced fluorescence spectroscopy analysis of kidney tissues: A pilot study for the identification of renal cell carcinoma.

    Pavithran M, Sanoop / Lukose, Jijo / Barik, Bijay Kumar / Periasami, Ammasi / Kartha, V B / Chawla, Arun / Chidangil, Santhosh

    Journal of biophotonics

    2023  Volume 16, Issue 11, Page(s) e202300021

    Abstract: The 325 nm-excited autofluorescence spectra from cancerous and normal renal tissues were collected ex vivo biopsy tissue samples, through an optical fiber probe-based system. Noticeable changes in intensity/wavelength were observed in the fluorescence ... ...

    Abstract The 325 nm-excited autofluorescence spectra from cancerous and normal renal tissues were collected ex vivo biopsy tissue samples, through an optical fiber probe-based system. Noticeable changes in intensity/wavelength were observed in the fluorescence emissions from endogenous fluorophores such as collagen, Nicotinamide adenine dinucleotide (NADH), Vitamin A (retinol), and flavin adenine dinucleotide, in pathological conditions with respect to the normal state. The energy metabolism involved in clear cell renal cell carcinoma (ccRCC) and chromophobe renal cell carcinoma (chRCC) are reflected in the fluorescence emission band at 445 nm due to bound NADH attributed to enhanced oxidative phosphorylation in chRCC and emission at 465 nm contributed by free NADH showing higher glycolytic action in ccRCC. The principal component analysis and one-way ANOVA effectively discriminate ccRCC from chRCC. It is shown that laser induced fluorescence technique with 325 nm excitation can be a suitable technique for optical pathology and in vivo surgical boundary demarcation in renal cell carcinoma.
    MeSH term(s) Humans ; Carcinoma, Renal Cell/diagnostic imaging ; Carcinoma, Renal Cell/pathology ; Pilot Projects ; Spectrometry, Fluorescence/methods ; NAD/analysis ; Kidney Neoplasms/diagnostic imaging ; Kidney Neoplasms/pathology ; Lasers ; Kidney/diagnostic imaging ; Kidney/pathology
    Chemical Substances NAD (0U46U6E8UK)
    Language English
    Publishing date 2023-09-02
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2390063-5
    ISSN 1864-0648 ; 1864-063X
    ISSN (online) 1864-0648
    ISSN 1864-063X
    DOI 10.1002/jbio.202300021
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Serum protein profile study of Myocardial Infarction using a LED Induced Fluorescence based HPLC system

    John, Reena V. / Devasia, Tom / Adigal, Sphurti S / Lukose, Jijo / Kartha, V.B. / Chidangil, Santhosh

    Journal of Chromatography B. 2023, p.123616-

    2023  , Page(s) 123616–

    Abstract: Cardiovascular diseases (CVDs) are the major health conditions for high mortality and morbidity in humans. Delay in the diagnosis of CVDs effect patients long and short-term health condition. In -house assembled UV-light emitting diode (LED) based ... ...

    Abstract Cardiovascular diseases (CVDs) are the major health conditions for high mortality and morbidity in humans. Delay in the diagnosis of CVDs effect patients long and short-term health condition. In -house assembled UV-light emitting diode (LED) based fluorescence detector for high -performance liquid chromatography (HPLC) (HPLC-LED-IF) system is used to record serum chromatograms of three categories of samples namely, before medicated- myocardial infarction (B-MI), after medicated- MI (A-MI), and normal. The sensitivity and performance of HPLC-LED-IF system is estimated using commercial serum proteins. Statistical analysis tools like, descriptive statistics, principal component analysis (PCA), and Match/ No Match test were applied to visualize the variation in three groups of samples. Statistical analysis of the protein profile data showed fairly good discrimination among the three categories. The receiver operating characteristic (ROC) curve also supported the reliability of the method to diagnose MI.
    Keywords blood proteins ; blood serum ; descriptive statistics ; diodes ; fluorescence ; liquid chromatography ; morbidity ; mortality ; myocardial infarction ; principal component analysis ; protein composition ; ultraviolet radiation ; LED- Induced Fluorescence ; High Performance Liquid Chromatography ; Diagnosis
    Language English
    Publishing place Elsevier B.V.
    Document type Article ; Online
    Note Pre-press version
    ZDB-ID 1180823-8
    ISSN 1570-0232 ; 1387-2273
    ISSN 1570-0232 ; 1387-2273
    DOI 10.1016/j.jchromb.2023.123616
    Database NAL-Catalogue (AGRICOLA)

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