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  1. Article ; Online: Real Life Evidence From the Use of COVID-19 mRNA Vaccines in Pediatric Populations.

    Lagousi, Theano / Tsagkli, Panagiota / Spoulou, Vana

    The Pediatric infectious disease journal

    2022  Volume 42, Issue 1, Page(s) e32–e34

    MeSH term(s) Child ; Humans ; COVID-19/prevention & control ; COVID-19 Vaccines
    Chemical Substances COVID-19 Vaccines
    Language English
    Publishing date 2022-09-14
    Publishing country United States
    Document type Journal Article
    ZDB-ID 392481-6
    ISSN 1532-0987 ; 0891-3668
    ISSN (online) 1532-0987
    ISSN 0891-3668
    DOI 10.1097/INF.0000000000003707
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1852: Show issues Location:
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  2. Article: Development of an Enzyme-Linked Immunosorbent Assay (ELISA) for Accurate and Prompt Coronavirus Disease 2019 (COVID-19) Diagnosis Using the Rational Selection of Serological Biomarkers.

    Lagousi, Theano / Routsias, John / Spoulou, Vana

    Diagnostics (Basel, Switzerland)

    2021  Volume 11, Issue 11

    Abstract: Prompt COVID-19 diagnosis is urgently required to support infection control measures. Currently available serological tests for measuring SARS-CoV-2 antibodies use different target antigens, although their sensitivity and specificity presents a challenge. ...

    Abstract Prompt COVID-19 diagnosis is urgently required to support infection control measures. Currently available serological tests for measuring SARS-CoV-2 antibodies use different target antigens, although their sensitivity and specificity presents a challenge. We aimed to develop an "in-house" serological ELISA to measure antibodies against SARS-CoV-2 by combining different protein antigens. Sera (
    Language English
    Publishing date 2021-10-23
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662336-5
    ISSN 2075-4418
    ISSN 2075-4418
    DOI 10.3390/diagnostics11111970
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  3. Article: Protecting the Offspring, the Gift of Maternal Immunization: Current Status and Future Perspectives.

    Lagousi, Theano / Gkentzi, Despoina / Geropeppa, Maria / Tsagkli, Panagiota / Spoulou, Vana

    Vaccines

    2022  Volume 10, Issue 11

    Abstract: Pregnancy is characterized by immunological alterations in pregnant women that permit the growth of a semi-allogenic fetus, resulting in greater susceptibility of childbearing women to infections. Furthermore, due to the immaturity of the immune system ... ...

    Abstract Pregnancy is characterized by immunological alterations in pregnant women that permit the growth of a semi-allogenic fetus, resulting in greater susceptibility of childbearing women to infections. Furthermore, due to the immaturity of the immune system of neonates, a protection gap is present in early life, leaving neonates and infants vulnerable to infectious diseases with increased morbidity and mortality. Maternal immunization against influenza, pertussis, and, in the context of the COVID-19 pandemic, SARS-CoV-2 has been implemented in several countries, with beneficial effects on both the mother and the offspring. The main protective mechanism of vaccination during pregnancy is transplacental transfer of maternal antibodies. However, recent evidence has implied that the fetal immune system may be influenced beyond passive immunity. This review sheds light on the current status of the routinely administered vaccinations during pregnancy, focusing on the impact of maternal immunization on the priming of the fetal immune system and suggesting future perspectives for the optimization of vaccination strategies.
    Language English
    Publishing date 2022-11-18
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2703319-3
    ISSN 2076-393X
    ISSN 2076-393X
    DOI 10.3390/vaccines10111953
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  4. Article ; Online: Paving the Way Towards Precision Vaccinology: The Paradigm of Myocarditis After Coronavirus Disease 2019 (COVID-19) Vaccination.

    Lagousi, Theano / Papadatou, Ioanna / Strempas, Petros / Chatzikalil, Elena / Spoulou, Vana

    Clinical infectious diseases : an official publication of the Infectious Diseases Society of America

    2022  Volume 75, Issue Supplement_1, Page(s) S18–S23

    Abstract: Systems vaccinology approaches have introduced novel tools for the evaluation of the safety profile of novel vaccine antigens by developing biomarkers of vaccine reactogenicity associated with potential adverse events. The use of such approaches may ... ...

    Abstract Systems vaccinology approaches have introduced novel tools for the evaluation of the safety profile of novel vaccine antigens by developing biomarkers of vaccine reactogenicity associated with potential adverse events. The use of such approaches may prove extremely advantageous in the context of a global pandemic where accelerated approval of new vaccine formulations for all ages is essential for the containment of the epidemic. The spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has had devastating effects on global health, but the emergency authorization of mRNA vaccines significantly reduced SARS-CoV-2-associated morbidity and mortality. Despite their favorable safety profile in adult populations, recent reports have raised concerns about an association of the mRNA-based vaccines with acute myocarditis, predominantly among male adolescents and young adults following the second vaccine dose. Here, we review data on myocarditis epidemiology following SARS-CoV-2 mRNA vaccination and describe potential mechanisms involved that may explain the sex- and age-related differences, focusing on mRNA immune reactivity. The case of vaccine-associated myocarditis highlights the need to incorporate precision vaccinology approaches for the development of safe and effective vaccines for everyone.
    MeSH term(s) Adolescent ; COVID-19/prevention & control ; Humans ; Male ; Myocarditis ; RNA, Messenger ; SARS-CoV-2 ; Vaccination/adverse effects ; Vaccinology ; Viral Vaccines ; mRNA Vaccines/adverse effects
    Chemical Substances RNA, Messenger ; Viral Vaccines ; mRNA Vaccines
    Language English
    Publishing date 2022-06-06
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 1099781-7
    ISSN 1537-6591 ; 1058-4838
    ISSN (online) 1537-6591
    ISSN 1058-4838
    DOI 10.1093/cid/ciac396
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1505: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)
    Zs.MO 480: Show issues

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  5. Article ; Online: Epidemiological Data, Serovar Distribution and Antimicrobial Resistance Patterns of Salmonella Species in Children, Greece 2011-2017: A Retrospective Study.

    Grivas, Grigoris / Lagousi, Theano / Mandilara, Georgia

    Acta medica academica

    2021  Volume 49, Issue 3, Page(s) 255–264

    Abstract: Objective: This study aimed to describe Salmonella epidemiology and antimicrobial resistance in Greek children over the period of 2011-2017.: Materials and methods: A 7-year retrospective study (2011-2017) was performed, based on data recorded by the ...

    Abstract Objective: This study aimed to describe Salmonella epidemiology and antimicrobial resistance in Greek children over the period of 2011-2017.
    Materials and methods: A 7-year retrospective study (2011-2017) was performed, based on data recorded by the National Reference Centre for Salmonella, among children aged ≤14 years. Epidemiological data, serovar distribution and antimicrobial resistance patterns were recorded.
    Results: Overall, 2347 Salmonella isolates were collected (27 typhoid-paratyphoid). Salmonellosis cases increased by almost 2-fold in 2017 compared to 2011. The highest rates were reported in August, with infants being the most vulnerable group (17.9%). The majority of isolates were identified in stool samples (91%). Boys slightly outnumbered girls (˜1.05:1). Salmonella Enteritidis was the most prevalent serovar (28.5%), followed by Salmonella Typhimurium (12.2%) and Salmonella monophasic Typhimurium (10.4%). Non-typhoid isolates displayed low resistance rates to 3rd generation cephalosporins (1%) and ciprofloxacin (0.3%), while the corresponding resistance of typhoid isolates was 10% and 5% respectively. An increasing trend of Salmonella monophasic Typhimurium was recorded, associated with high rates of multidrug resistance, reaching a percentage of 97.8% in 2017.
    Conclusions: Salmonellosis epidemiology in Greek children is comparable to previously published European data. Antimicrobial resistance rates to 3rd-generation cephalosporins and ciprofloxacin for non-typhoid and typhoid-paratyphoid remain low. Notably, there is an increasing prevalence of Salmonella monophasic Typhimurium isolates, associated with multiple antimicrobial resistance.
    MeSH term(s) Anti-Bacterial Agents/pharmacology ; Child ; Drug Resistance, Bacterial ; Female ; Greece/epidemiology ; Humans ; Infant ; Male ; Microbial Sensitivity Tests ; Retrospective Studies ; Salmonella ; Serogroup
    Chemical Substances Anti-Bacterial Agents
    Language English
    Publishing date 2021-03-29
    Publishing country Bosnia and Herzegovina
    Document type Journal Article
    ZDB-ID 2558604-X
    ISSN 1840-2879 ; 1840-1848
    ISSN (online) 1840-2879
    ISSN 1840-1848
    DOI 10.5644/ama2006-124.315
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  6. Article ; Online: Development of an Enzyme-Linked Immunosorbent Assay (ELISA) for Accurate and Prompt Coronavirus Disease 2019 (COVID-19) Diagnosis Using the Rational Selection of Serological Biomarkers

    Theano Lagousi / John Routsias / Vana Spoulou

    Diagnostics, Vol 11, Iss 1970, p

    2021  Volume 1970

    Abstract: Prompt COVID-19 diagnosis is urgently required to support infection control measures. Currently available serological tests for measuring SARS-CoV-2 antibodies use different target antigens, although their sensitivity and specificity presents a challenge. ...

    Abstract Prompt COVID-19 diagnosis is urgently required to support infection control measures. Currently available serological tests for measuring SARS-CoV-2 antibodies use different target antigens, although their sensitivity and specificity presents a challenge. We aimed to develop an “in-house” serological ELISA to measure antibodies against SARS-CoV-2 by combining different protein antigens. Sera ( n = 44) from COVID-19-confirmed patients were evaluated against different SARS-CoV-2 protein antigens and all potential combinations using ELISA. Patients’ sera were also evaluated against commercially available ELISA diagnostic kits. The mixture containing RBD 2.5 μg/mL, S2 1 μg/mL and N 1.5 μg/mL was found to be the most potent. Plates were incubated with patients’ sera (1:100), and goat anti-human alkaline phosphatase-conjugated IgG, ΙgM and IgA antibody was added. The cut-off value for each assay was determined using the mean optical density plus two standard deviations of pre-pandemic controls. The “in-house” ELISA displayed 91% sensitivity and 97% specificity for IgG antibodies, whereas its sensitivity and specificity for IgM and IgA were 75% and 95% and 73% and 91%, respectively. The “in-house” ELISA developed here combined three SARS-CoV-2 antigens (RBD, S2 and N) as capture antigens and displayed comparable and even higher sensitivity and specificity than otherwise quite reliable commercially available ELISA diagnostic kits.
    Keywords SARS-CoV-2 ; antibodies ; serological tests ; Medicine (General) ; R5-920
    Subject code 616
    Language English
    Publishing date 2021-10-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  7. Article: SARS-CoV-2 mRNA Dual Immunization Induces Innate Transcriptional Signatures, Establishes T-Cell Memory and Coordinates the Recall Response.

    Papadatou, Ioanna / Geropeppa, Maria / Verrou, Kleio-Maria / Tzanoudaki, Marianna / Lagousi, Theano / Liatsis, Emmanouil / Spoulou, Vana

    Vaccines

    2023  Volume 11, Issue 1

    Abstract: Background: mRNA vaccines have played a crucial role in controlling the SARS-CoV-2 global pandemic. However, the immunological mechanisms involved in the induction, magnitude and longevity of mRNA-vaccine-induced protective immunity are still unclear.!## ...

    Abstract Background: mRNA vaccines have played a crucial role in controlling the SARS-CoV-2 global pandemic. However, the immunological mechanisms involved in the induction, magnitude and longevity of mRNA-vaccine-induced protective immunity are still unclear.
    Methods: In our study, we used whole-RNA sequencing along with detailed immunophenotyping of antigen-specific T cells and humoral RBD-specific response to dual immunization with the Pfizer-BioNTech mRNA vaccine (BNT162b2) and correlated them with response to an additional dose, administered 10 months later, in order to comprehensively profile the immune response of healthy volunteers to BNT162b2.
    Results: Primary dual immunization induced upregulation of the Type I interferon pathway and generated spike protein (S)-specific IFN-γ+ and TNF-α+ CD4 T cells, S-specific memory CD4 T cells, and RBD-specific antibodies against SARS-CoV-2. S-specific CD4 T cells induced by the primary series correlated with the RBD-specific antibody titers to a third dose.
    Conclusions: This study demonstrates the induction of both innate and adaptive immunity in response to the BNT162b2 mRNA vaccine in a coordinated manner and identifies the central role of primarily induced CD4+ T cells as a predictive biomarker of the magnitude of anamnestic immune response.
    Language English
    Publishing date 2023-01-01
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2703319-3
    ISSN 2076-393X
    ISSN 2076-393X
    DOI 10.3390/vaccines11010103
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  8. Article: Comparative Characterization of Human Antibody Response Induced by BNT162b2 Vaccination vs. SARS-CoV-2 Wild-Type Infection.

    Lagousi, Theano / Routsias, John / Mavrouli, Maria / Papadatou, Ioanna / Geropeppa, Maria / Spoulou, Vana

    Vaccines

    2022  Volume 10, Issue 8

    Abstract: Humoral immunity after SARS-CoV-2 immunization or natural infection is thought to be evanescent. In our study, we aimed to longitudinally characterize the kinetics of antibody titers after dual BNT162b2 immunization or wild-type infection. Vaccinated and ...

    Abstract Humoral immunity after SARS-CoV-2 immunization or natural infection is thought to be evanescent. In our study, we aimed to longitudinally characterize the kinetics of antibody titers after dual BNT162b2 immunization or wild-type infection. Vaccinated and recovered individuals displayed distinct antibody kinetics, as convalescents had detectable RBD-, S1-specific, and neutralizing IgG antibody titers two weeks post-infection that gradually increased longitudinally, while RBD-, S1-specific, and neutralizing IgG were detected in vaccinees after the first dose, increased significantly 3 weeks post the second dose and decreased significantly 4-5 months thereafter. Neutralizing IgG was significantly higher initially in convalescent individuals; however, vaccines displayed significantly higher neutralizing antibodies 4-5 months post the second dose. In both groups, there was a strong negative association between elapsed time and antibody levels. The avidity of anti-RBD antibody titers increased significantly in patients longitudinally, while in vaccinees initially increased, with subsequent decrease, remaining however higher than antibody avidity of recovered individuals at all time-points. Anti-RBD antibodies were strongly correlated with neutralizing and anti-S1 antibodies in both groups at all time-points. This study facilitates our further understanding of immune response to SARS-CoV-2 and vaccines.
    Language English
    Publishing date 2022-07-29
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2703319-3
    ISSN 2076-393X
    ISSN 2076-393X
    DOI 10.3390/vaccines10081210
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  9. Article: Pneumococcal Immunization Strategies for High-Risk Pediatric Populations Worldwide: One Size Does Not Fit All.

    Lagousi, Theano / Papadatou, Ioanna / Strempas, Petros / Chatzikalil, Elena / Spoulou, Vana

    Vaccines

    2021  Volume 9, Issue 12

    Abstract: Despite the significant reduction in pneumococcal disease due to pneumococcal vaccines, protection of vulnerable high-risk individuals, especially pediatric populations, remains a great challenge. In an effort to maximize the protection of high-risk ... ...

    Abstract Despite the significant reduction in pneumococcal disease due to pneumococcal vaccines, protection of vulnerable high-risk individuals, especially pediatric populations, remains a great challenge. In an effort to maximize the protection of high-risk children against pneumococcal disease, a combined schedule that includes both conjugate and polysaccharide vaccines is recommended by several countries in the developed world. On the other hand, middle- and low-income countries do not have in place established policies for pneumococcal immunization of children at risk. Pneumococcal conjugate vaccines, despite their benefits, have several limitations, mainly associated with serotype replacement and the wide range of serotype coverage worldwide. In addition, PPV23-impaired immunogenicity and the hyporesponsiveness effect among populations at risk have been well-documented. Therefore, the added value of continuing to include PPV23 in vaccination schedules for high-risk individuals in the years to come remains to be determined by monitoring whether the replacing/remaining serotypes causing IPD are covered by PPV23 to determine whether its benefits outweigh its limitations. In this review, we aim to describe serotype distribution and vaccine efficacy data on pneumococcal disease in the pre- and post-PCV implementation era among high-risk children in both developed and developing countries, assessing the optimization of current recommendations for their vaccination against pneumococcal disease.
    Language English
    Publishing date 2021-11-24
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2703319-3
    ISSN 2076-393X
    ISSN 2076-393X
    DOI 10.3390/vaccines9121390
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  10. Article ; Online: Understanding host immune responses to pneumococcal proteins in the upper respiratory tract to develop serotype-independent pneumococcal vaccines.

    Lagousi, Theano / Basdeki, Paraskevi / De Jonge, Marien I / Spoulou, Vana

    Expert review of vaccines

    2020  Volume 19, Issue 10, Page(s) 959–972

    Abstract: ... ...

    Abstract Introduction
    MeSH term(s) Administration, Intranasal ; Animals ; Bacterial Proteins/immunology ; Humans ; Immunity, Mucosal ; Nasopharynx/immunology ; Nasopharynx/microbiology ; Pneumococcal Infections/immunology ; Pneumococcal Infections/microbiology ; Pneumococcal Infections/prevention & control ; Pneumococcal Vaccines/administration & dosage ; Pneumococcal Vaccines/immunology ; Respiratory System/immunology ; Respiratory System/microbiology ; Serogroup ; Streptococcus pneumoniae/immunology
    Chemical Substances Bacterial Proteins ; Pneumococcal Vaccines
    Language English
    Publishing date 2020-11-08
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 2181284-6
    ISSN 1744-8395 ; 1476-0584
    ISSN (online) 1744-8395
    ISSN 1476-0584
    DOI 10.1080/14760584.2020.1843433
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 6077: Show issues Location:
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