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  1. Article ; Online: Improving RDS treatment with current drugs.

    Smart, David E / Princivalle, Marc B

    The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians

    2012  Volume 25, Issue 8, Page(s) 1209–1211

    Abstract: Currently attributed to a lack of foetal lung development, respiratory distress syndrome is the eighth largest cause of infant mortality (USA). Corticosteroids have proved successful but are not infallible in this indication having both a 24-hour latency ...

    Abstract Currently attributed to a lack of foetal lung development, respiratory distress syndrome is the eighth largest cause of infant mortality (USA). Corticosteroids have proved successful but are not infallible in this indication having both a 24-hour latency and little effect on surfactant production. In vivo evidence shows a triggering event in vaginal delivery leads to a rapid final preparation of the lungs, accelerating fluid re-adsorption and surfactant production. It may be possible to reproduce accelerated adaptation synergistically with natural and steroidal maturation; however this would require looking again at β-agonists. Vulnerable pregnancies may be better served by corticosteroids, oxytocin tocolytics. A single dose of a β-agonist immediately before delivery may maximise adaptation while avoiding previous failings of this therapy. Trends in premature birth and caesarean section, make prevention of this syndrome increasingly challenging, however room for improvement may be possible with current therapies.
    MeSH term(s) Adrenal Cortex Hormones/therapeutic use ; Drug Therapy/methods ; Drug Therapy/trends ; Humans ; Incidence ; Infant, Newborn ; Pharmaceutical Preparations ; Pulmonary Surfactants/therapeutic use ; Respiratory Distress Syndrome, Newborn/drug therapy ; Respiratory Distress Syndrome, Newborn/epidemiology
    Chemical Substances Adrenal Cortex Hormones ; Pharmaceutical Preparations ; Pulmonary Surfactants
    Language English
    Publishing date 2012-08
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 2077261-0
    ISSN 1476-4954 ; 1057-0802 ; 1476-7058
    ISSN (online) 1476-4954
    ISSN 1057-0802 ; 1476-7058
    DOI 10.3109/14767058.2011.634456
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 3510: Show issues Location:
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  2. Article: New insight on element bioaccumulation performance of two lichen biomonitors: When morpho-chemical details mark the difference

    Fortuna, Lorenzo / Adami, Gianpiero / Princivalle, Francesco / Tretiach, Mauro

    Science of the total environment. 2021 Aug. 15, v. 782

    2021  

    Abstract: In biomonitoring of airborne elements with native lichens, two allopatric species could be used jointly only when bioaccumulation mechanisms are the same, and differences negligible. The present study aims at (i) investigating the bioaccumulation ... ...

    Abstract In biomonitoring of airborne elements with native lichens, two allopatric species could be used jointly only when bioaccumulation mechanisms are the same, and differences negligible. The present study aims at (i) investigating the bioaccumulation performance for 12 elements in one-year-old lobes of Flavoparmelia caperata and Xanthoria parietina (i.e. the two lichen native biomonitors most used in the peri-Mediterranean regions) collected side-by-side on the same trees at 17 sampling sites. Both species were characterized morphologically and by X-ray diffraction analysis to evaluate specific thallus area and oxalate content, respectively. Results show that Ca and chalcophile elements (Cd, Cu, Pb and Zn) were more concentrated in F. caperata, lithophile and siderophile elements (Cr, Fe, and Ti) in X. parietina, whereas Al, Li, Mn, and Ni were accumulated in similar amounts. The outstanding content of Ca-oxalate deposits in F. caperata underlies the higher content of Ca, Cd and Pb observed in this species. On the contrary, the micro-anatomy of the upper cortex of X. parietina, which is more porous than in F. caperata, allows a higher transfer of litophile and siderophile elements. The adoption of interspecific calibration factors for biomonitoring purposes is thoroughly discussed, and the choice of using a single native species made by the most recent guidelines for biomonitoring airborne elements with lichens is fully supported.
    Keywords X-ray diffraction ; Xanthoria ; allopatry ; bioaccumulation ; calcium oxalate ; cortex ; environment ; environmental monitoring ; indicator species ; indigenous species ; lichens ; thallus
    Language English
    Dates of publication 2021-0815
    Publishing place Elsevier B.V.
    Document type Article
    Note NAL-AP-2-clean
    ZDB-ID 121506-1
    ISSN 1879-1026 ; 0048-9697
    ISSN (online) 1879-1026
    ISSN 0048-9697
    DOI 10.1016/j.scitotenv.2021.146360
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  3. Article: Developmental roles of heparan sulfate proteoglycans: a comparative review in Drosophila, mouse and human.

    Princivalle, Marc / de Agostini, Ariane

    The International journal of developmental biology

    2002  Volume 46, Issue 3, Page(s) 267–278

    Abstract: In recent years, progress in the fields of development and proteoglycan biology have produced converging evidence of the role of proteoglycans in morphogenesis. Numerous studies have demonstrated that proteoglycans are involved in several distinct ... ...

    Abstract In recent years, progress in the fields of development and proteoglycan biology have produced converging evidence of the role of proteoglycans in morphogenesis. Numerous studies have demonstrated that proteoglycans are involved in several distinct morphogenetic pathways upon which they act at different levels. In particular, proteoglycans can determine the generation of morphogen gradients and be required for their signal transduction. The surface of most cells and the extracellular matrix are decorated by heparan sulfates which are the most common glycosaminoglycans, normally present as heparan sulfate proteoglycans. Considerable structural heterogeneity is generated in proteoglycans by the biosynthetic modification of their heparan sulfate chains as well as by the diverse nature of their different core proteins. This heterogeneity provides an impressive potential for protein-protein and protein-carbohydrate interactions, and can partly explain the diversity of proteoglycan involvement in different morphogenetic pathways. In this review, we summarize the current knowledge about mutations affecting heparan sulfate proteoglycans that influence the function of growth factor pathways essential for tissue assembly, differentiation and development. The comparison of data obtained in Drosophila, rodents and humans reveals that mutations affecting the proteoglycan core proteins or one of the biosynthetic enzymes of their heparan sulfate chains have profound effects on growth and morphogenesis. Further research will complete the picture, but current evidence shows that at the very least, heparan sulfate proteoglycans need to be counted as legitimate elements of morphogenetic pathways that have been maintained throughout evolution as determinant mechanisms of pattern formation.
    MeSH term(s) Aging ; Animals ; Drosophila/metabolism ; Glycosaminoglycans/biosynthesis ; Heparan Sulfate Proteoglycans/physiology ; Humans ; Mice ; Proteoglycans/biosynthesis ; Species Specificity
    Chemical Substances Glycosaminoglycans ; Heparan Sulfate Proteoglycans ; Proteoglycans
    Language English
    Publishing date 2002-05
    Publishing country Spain
    Document type Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 1036070-0
    ISSN 1696-3547 ; 0214-6282
    ISSN (online) 1696-3547
    ISSN 0214-6282
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 3202: Show issues Location:
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  4. Article ; Online: A differential cytokine expression profile is induced by highly purified human menopausal gonadotropin and recombinant follicle-stimulating hormone in a pre- and postovulatory mouse follicle culture model.

    Foster, Rebecca / Segers, Ingrid / Smart, David / Adriaenssens, Tom / Smitz, Johan / Arce, Joan-Carles / Princivalle, Marc

    Fertility and sterility

    2010  Volume 93, Issue 5, Page(s) 1464–1476

    Abstract: Objective: To compare the differential effects of highly purified (HP) hMG or recombinant FSH (rFSH) on cytokine expression before and after ovulation in an in vitro mouse ovarian follicle model.: Design: A prospective laboratory in vitro study.: ... ...

    Abstract Objective: To compare the differential effects of highly purified (HP) hMG or recombinant FSH (rFSH) on cytokine expression before and after ovulation in an in vitro mouse ovarian follicle model.
    Design: A prospective laboratory in vitro study.
    Setting: A university-based reproductive biology laboratory. MATERIAL(S): Mechanically isolated mouse preantral follicles from 14-day-old prepubertal mouse ovaries (F1 hybrids: C57BL/6JxCBA/ca).
    Intervention(s): Randomly distributed mouse early preantral follicles were exposed to two hyperstimulation conditions with either HP-hMG or rFSH. An ovulatory stimulus was given using hCG/epidermal growth factor. Conditioned media from the two culture conditions were collected on the days before and after in vitro ovulation. Conditioned media were compared for their relative cytokine profile content as measured by a cytokine antibody array analysis.
    Main outcome measure(s): Relative concentrations of 62 cytokines in conditioned media before and after ovulation.
    Result(s): Statistically significant increase in the production of a number of cytokines was found after HP-hMG stimulation compared with rFSH: 14 and 24 pre- and post-rhCG, respectively. Cytokines with the largest significant difference (more than 5 times) before and after ovulation included thymus-expressed cytokine (TECK), sTNFRI, and SDF-1alpha. The cytokines that are most strongly related to oocyte and embryo quality and implantation and that have been related to oocyte yield and maturation were significantly higher with HP-hMG.
    Conclusion(s): The significant differences in follicular cytokine production induced by HP-HMG and rFSH before and after in vitro ovulation might explain the difference in treatment outcome.
    MeSH term(s) Animals ; Cells, Cultured ; Crosses, Genetic ; Culture Media, Conditioned/metabolism ; Cytokines/metabolism ; Female ; Fertility Agents, Female/pharmacology ; Follicle Stimulating Hormone/pharmacology ; Humans ; Menotropins/pharmacology ; Mice ; Mice, Inbred C57BL ; Mice, Inbred DBA ; Ovarian Follicle/drug effects ; Ovarian Follicle/immunology ; Ovulation Induction/methods ; Protein Array Analysis ; Recombinant Proteins/pharmacology ; Time Factors
    Chemical Substances Culture Media, Conditioned ; Cytokines ; Fertility Agents, Female ; Recombinant Proteins ; Menotropins (61489-71-2) ; Follicle Stimulating Hormone (9002-68-0)
    Language English
    Publishing date 2010-03-15
    Publishing country United States
    Document type Comparative Study ; Journal Article
    ZDB-ID 80133-1
    ISSN 1556-5653 ; 0015-0282
    ISSN (online) 1556-5653
    ISSN 0015-0282
    DOI 10.1016/j.fertnstert.2009.01.136
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 73: Show issues Location:
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  5. Article ; Online: Relaxin modulates human and rat hepatic myofibroblast function and ameliorates portal hypertension in vivo.

    Fallowfield, Jonathan A / Hayden, Annette L / Snowdon, Victoria K / Aucott, Rebecca L / Stutchfield, Ben M / Mole, Damian J / Pellicoro, Antonella / Gordon-Walker, Timothy T / Henke, Alexander / Schrader, Joerg / Trivedi, Palak J / Princivalle, Marc / Forbes, Stuart J / Collins, Jane E / Iredale, John P

    Hepatology (Baltimore, Md.)

    2014  Volume 59, Issue 4, Page(s) 1492–1504

    Abstract: Unlabelled: Active myofibroblast (MF) contraction contributes significantly to the increased intrahepatic vascular resistance that is the primary cause of portal hypertension (PHT) in cirrhosis. We sought proof of concept for direct therapeutic ... ...

    Abstract Unlabelled: Active myofibroblast (MF) contraction contributes significantly to the increased intrahepatic vascular resistance that is the primary cause of portal hypertension (PHT) in cirrhosis. We sought proof of concept for direct therapeutic targeting of the dynamic component of PHT and markers of MF activation using short-term administration of the peptide hormone relaxin (RLN). We defined the portal hypotensive effect in rat models of sinusoidal PHT and the expression, activity, and function of the RLN-receptor signaling axis in human liver MFs. The effects of RLN were studied after 8 and 16 weeks carbon tetrachloride intoxication, following bile duct ligation, and in tissue culture models. Hemodynamic changes were analyzed by direct cannulation, perivascular flowprobe, indocyanine green imaging, and functional magnetic resonance imaging. Serum and hepatic nitric oxide (NO) levels were determined by immunoassay. Hepatic inflammation was assessed by histology and serum markers and fibrosis by collagen proportionate area. Gene expression was analyzed by quantitative reverse-transcription polymerase chain reaction (qRT-PCR) and western blotting and hepatic stellate cell (HSC)-MF contractility by gel contraction assay. Increased expression of RLN receptor (RXFP1) was shown in HSC-MFs and fibrotic liver diseases in both rats and humans. RLN induced a selective and significant reduction in portal pressure in pathologically distinct PHT models, through augmentation of intrahepatic NO signaling and a dramatic reduction in contractile filament expression in HSC-MFs. Critical for translation, RLN did not induce systemic hypotension even in advanced cirrhosis models. Portal blood flow and hepatic oxygenation were increased by RLN in early cirrhosis. Treatment of human HSC-MFs with RLN inhibited contractility and induced an antifibrogenic phenotype in an RXFP1-dependent manner.
    Conclusion: We identified RXFP1 as a potential new therapeutic target for PHT and MF activation status.
    MeSH term(s) Actins/metabolism ; Animals ; Carbon Tetrachloride/adverse effects ; Cells, Cultured ; Desmin/metabolism ; Disease Models, Animal ; Glial Fibrillary Acidic Protein/metabolism ; Hemodynamics/drug effects ; Hemodynamics/physiology ; Humans ; Hypertension, Portal/chemically induced ; Hypertension, Portal/physiopathology ; Hypertension, Portal/prevention & control ; Liver/drug effects ; Liver/metabolism ; Liver/physiopathology ; Liver Cirrhosis/chemically induced ; Liver Cirrhosis/physiopathology ; Liver Cirrhosis/prevention & control ; Male ; Myofibroblasts/drug effects ; Myofibroblasts/pathology ; Myofibroblasts/physiology ; Nitric Oxide/metabolism ; Rats ; Rats, Sprague-Dawley ; Receptors, G-Protein-Coupled/metabolism ; Receptors, Peptide/metabolism ; Relaxin/metabolism ; Relaxin/pharmacology ; Relaxin/therapeutic use
    Chemical Substances Actins ; Desmin ; Glial Fibrillary Acidic Protein ; RXFP1 protein, human ; Receptors, G-Protein-Coupled ; Receptors, Peptide ; Rxfp1 protein, rat ; smooth muscle actin, rat ; Nitric Oxide (31C4KY9ESH) ; Relaxin (9002-69-1) ; Carbon Tetrachloride (CL2T97X0V0)
    Language English
    Publishing date 2014-03-03
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 604603-4
    ISSN 1527-3350 ; 0270-9139
    ISSN (online) 1527-3350
    ISSN 0270-9139
    DOI 10.1002/hep.26627
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1660: Show issues Location:
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  6. Article: A novel, simple bioactivity assay for relaxin based on inhibition of platelet aggregation.

    Bani, Daniele / Nistri, Silvia / Cinci, Lorenzo / Giannini, Lucia / Princivalle, Marc / Elliott, Lucy / Bigazzi, Mario / Masini, Emanuela

    Regulatory peptides

    2007  Volume 144, Issue 1-3, Page(s) 10–16

    Abstract: In humans, the relaxin hormone family includes H1, H2 and H3 isoforms and insulin-like peptides 3 to 6. The ever-increasing interest in relaxin as potential new drug requires reliable methods to compare bioactivity of different relaxins. The existing ... ...

    Abstract In humans, the relaxin hormone family includes H1, H2 and H3 isoforms and insulin-like peptides 3 to 6. The ever-increasing interest in relaxin as potential new drug requires reliable methods to compare bioactivity of different relaxins. The existing bioassays include in vivo or ex vivo methods evaluating the organ-specific responses to relaxin and in vitro methods based on measurement of cAMP increase in relaxin receptor-bearing cells. We previously demonstrated that relaxin dose-dependently inhibits platelet aggregation. On this basis, we have developed a simple, reliable bioassay for relaxin used to compare purified porcine relaxin, assumed as reference standard, with two recombinant human H2 relaxins, H3 relaxin, insulin-like peptides 3 and 5. Pre-incubation of platelets with relaxins (3, 10, 30,100, 300 ng/ml; 10 min.) caused the inhibition of ADP-induced platelet aggregation. Within the 10-100 ng/ml range, porcine relaxin showed the highest effects and a nearly linear dose-response correlation. Lower peptide concentrations were ineffective, as were insulin-like peptides 3 and 5 at any concentration assayed. Platelet inhibition was mediated by specific RXFP1 relaxin receptor and cGMP, whose intracellular levels dose-dependently increased upon relaxin. For comparison, we stimulated THP-1 cells, a relaxin receptor-bearing cell line, with porcine relaxin, human H2 and H3 relaxins at the above concentrations (15 min.). We observed a dose-related increase of intracellular cAMP similar to the trend of platelet inhibition. Insulin like peptide 5 was ineffective. In conclusion, this study shows that inhibition of platelet aggregation may be used to assess bioactivity of relaxin preparations for experimental and clinical purposes.
    MeSH term(s) Biological Assay/methods ; Cell Line ; Cyclic GMP/metabolism ; Dose-Response Relationship, Drug ; Humans ; Platelet Aggregation/drug effects ; RNA, Messenger/metabolism ; Receptors, G-Protein-Coupled/genetics ; Receptors, G-Protein-Coupled/metabolism ; Receptors, Peptide/genetics ; Receptors, Peptide/metabolism ; Relaxin/metabolism ; Relaxin/pharmacology
    Chemical Substances RNA, Messenger ; Receptors, G-Protein-Coupled ; Receptors, Peptide ; relaxin receptors ; Relaxin (9002-69-1) ; Cyclic GMP (H2D2X058MU)
    Language English
    Publishing date 2007-12-04
    Publishing country Netherlands
    Document type Comparative Study ; Evaluation Studies ; Journal Article
    ZDB-ID 225685-x
    ISSN 1873-1686 ; 0167-0115
    ISSN (online) 1873-1686
    ISSN 0167-0115
    DOI 10.1016/j.regpep.2007.05.004
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1593: Show issues Location:
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  7. Article: Rapid suppression of plasma testosterone levels and tumor growth in the dunning rat model treated with degarelix, a new gonadotropin-releasing hormone antagonist.

    Princivalle, Marc / Broqua, Pierre / White, Richard / Meyer, Jessica / Mayer, Gaell / Elliott, Lucy / Bjarnason, Ketil / Haigh, Robert / Yea, Christopher

    The Journal of pharmacology and experimental therapeutics

    2007  Volume 320, Issue 3, Page(s) 1113–1118

    Abstract: Degarelix (FE 200486) is a member of a new class of water-soluble (>50 mg/ml) gonadotropin-releasing hormone (GnRH) antagonists in clinical development for prostate cancer. Upon subcutaneous administration, degarelix forms a gel that results in a ... ...

    Abstract Degarelix (FE 200486) is a member of a new class of water-soluble (>50 mg/ml) gonadotropin-releasing hormone (GnRH) antagonists in clinical development for prostate cancer. Upon subcutaneous administration, degarelix forms a gel that results in a sustained release of the compound into the circulation, immediately blocking GnRH receptors in the pituitary and inducing a fast and sustained suppression of gonadotrophin secretion in rats and primates. One of the few animal models of prostate adenocarcinoma is the Dunning R-3327H rat carcinoma transplanted into Copenhagen rats. The growth of the Dunning tumor can be inhibited by various treatments reported to be effective in the clinic, such as GnRH superagonists, antiandrogens, 5-alphareductase inhibitors, tyrosine kinase inhibitors, and surgical castration. We report in this study that degarelix produces a fast and sustained suppression of the pituitary gonadal axis in rats and a similar inhibition of tumor growth compared with surgical castration in the Dunning R-3327H rat carcinoma model. First, degarelix as been compared with d-Trp(6)-luteinizing hormone-releasing hormone and surgical castration on a short-term study (2 months); and second, degarelix has been compared with leuprolide and surgical castration on a long-term study (12 months). In both studies, degarelix demonstrated a sustained inhibition of tumor growth at least comparable with surgical castration. These data provide a convincing profile of degarelix as a potential candidate for the clinical management of sex steroid-dependent pathologies, such as prostate cancer, where long-term reversible chemical castration is required.
    MeSH term(s) Animals ; Antineoplastic Agents/administration & dosage ; Antineoplastic Agents/pharmacology ; Antineoplastic Agents/therapeutic use ; Castration ; Gonadotropin-Releasing Hormone/antagonists & inhibitors ; Leuprolide/administration & dosage ; Leuprolide/pharmacology ; Leuprolide/therapeutic use ; Male ; Neoplasm Transplantation ; Oligopeptides/administration & dosage ; Oligopeptides/pharmacology ; Oligopeptides/therapeutic use ; Organ Size/drug effects ; Prostatic Neoplasms/blood ; Prostatic Neoplasms/drug therapy ; Prostatic Neoplasms/metabolism ; Prostatic Neoplasms/pathology ; Rats ; Rats, Inbred Strains ; Testis/drug effects ; Testis/pathology ; Testosterone/blood
    Chemical Substances Antineoplastic Agents ; Oligopeptides ; acetyl-2-naphthylalanyl-3-chlorophenylalanyl-1-oxohexadecyl-seryl-4-aminophenylalanyl(hydroorotyl)-4-aminophenylalanyl(carbamoyl)-leucyl-ILys-prolyl-alaninamide ; Gonadotropin-Releasing Hormone (33515-09-2) ; Testosterone (3XMK78S47O) ; Leuprolide (EFY6W0M8TG)
    Language English
    Publishing date 2007-03
    Publishing country United States
    Document type Journal Article
    ZDB-ID 3106-9
    ISSN 1521-0103 ; 0022-3565
    ISSN (online) 1521-0103
    ISSN 0022-3565
    DOI 10.1124/jpet.106.112326
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  8. Article: Coordinate expression of anticoagulant heparan sulfate proteoglycans and serine protease inhibitors in the rat ovary: a potent system of proteolysis control.

    Hasan, Shereen / Hosseini, Ghamartaj / Princivalle, Marc / Dong, Ji-Cui / Birsan, Daniela / Cagide, Cristina / de Agostini, Ariane I

    Biology of reproduction

    2002  Volume 66, Issue 1, Page(s) 144–158

    Abstract: During the reproductive cycle, ovarian follicles undergo major tissue-remodeling involving vascular changes and proteolysis. Anticoagulant heparan sulfate proteoglycans (aHSPGs) are expressed by granulosa cells during the development of the ovarian ... ...

    Abstract During the reproductive cycle, ovarian follicles undergo major tissue-remodeling involving vascular changes and proteolysis. Anticoagulant heparan sulfate proteoglycans (aHSPGs) are expressed by granulosa cells during the development of the ovarian follicle. The function of aHSPGs in the ovary is unknown, but they might be involved in proteolysis control through binding and activation of serine protease inhibitors. To identify functional interactions between aHSPGs and heparin-binding protease inhibitors in the follicle, we have coordinately localized aHSPGs, antithrombin III, protease nexin-1, and plasminogen activator inhibitor-1 in the rat ovary during natural and gonadotropin-stimulated cycles. Anticoagulant HSPGs were visualized by autoradiography of cryosections incubated with 125I-antithrombin III, and protease inhibitors were assessed by immunohistochemistry and Northern blot hybridization. Anticoagulant HSPGs were expressed in follicles before ovulation, were transiently decreased in postovulatory follicles, and were abundant in the corpus luteum, mainly on capillaries. Anticoagulant HSPGs were colocalized with protease nexin-1 in follicles from the early antral stage until ovulation, with antithrombin III in the preovulatory stage and after ovulation, and with plasminogen activator inhibitor-1 in the corpus luteum. These data demonstrate that aHSPGs are critically expressed in the ovary to interact sequentially with protease nexin-1, antithrombin III, and plasminogen activator inhibitor-1 during the cycle. The specificity of these inhibitors is shifted toward thrombin inhibition in the presence of heparin, suggesting that aHSPGs direct their action to control fibrin deposition in the follicle. The occupation of aHSPGs antithrombin-binding sites by mutant R393C antithrombin III, injected in the ovarian bursa, decreased ovulation efficiency, further supporting the involvement of aHSPGs in the ovulation process.
    MeSH term(s) Amyloid beta-Protein Precursor ; Animals ; Anticoagulants/blood ; Blotting, Northern ; Carrier Proteins/metabolism ; Cell Line ; Cricetinae ; Estrous Cycle/physiology ; Female ; Fibrin/metabolism ; Granulosa Cells/metabolism ; Heparan Sulfate Proteoglycans/biosynthesis ; Heparan Sulfate Proteoglycans/physiology ; Immunohistochemistry ; Ovary/metabolism ; Ovulation/physiology ; Plasminogen Activator Inhibitor 1/biosynthesis ; Protease Nexins ; RNA/isolation & purification ; Rats ; Rats, Sprague-Dawley ; Receptors, Cell Surface ; Serine Proteinase Inhibitors/biosynthesis ; Serpins/biosynthesis
    Chemical Substances Amyloid beta-Protein Precursor ; Anticoagulants ; Carrier Proteins ; Heparan Sulfate Proteoglycans ; Plasminogen Activator Inhibitor 1 ; Protease Nexins ; Receptors, Cell Surface ; Serine Proteinase Inhibitors ; Serpins ; RNA (63231-63-0) ; Fibrin (9001-31-4)
    Language English
    Publishing date 2002-01
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1118-6
    ISSN 1529-7268 ; 0006-3363
    ISSN (online) 1529-7268
    ISSN 0006-3363
    DOI 10.1095/biolreprod66.1.144
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 551: Show issues Location:
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  9. Article: Layer-specific immunocytochemical localization of GABA(B)R1a and GABA(B)R1b receptors in the rat piriform cortex.

    Princivalle, A / Spreafico, R / Bowery, N / De Curtis, M

    The European journal of neuroscience

    2000  Volume 12, Issue 4, Page(s) 1516–1520

    Abstract: ... antibodies is present in the piriform cortex of adult rats. The GABABR1a antibody selectively marked ...

    Abstract A peculiar, layer-segregated immunoreactive distribution of GABABR1a and GABABR1b receptor antibodies is present in the piriform cortex of adult rats. The GABABR1a antibody selectively marked the neuropile in layer Ia, where afferent olfactory fibres and intrinsic GABAergic (gamma-aminobutyric acid) axons terminate on the distal apical dendrites of pyramidal neurons. The GABABR1b antibody was detected in the soma and the large basal dendrites of layer II and III neurons. The pattern of distribution observed supports the hypothesis that (presynaptic) GABABR1a receptors in the superficial molecular layer modulate neurotransmitter release in a feedforward synaptic circuit, whereas GABABR1b (postsynaptic) receptors mediate feedback inhibitory potentials on principal cells.
    MeSH term(s) Animals ; Antibodies ; Immunohistochemistry ; Molecular Weight ; Neuropil/chemistry ; Olfactory Pathways/chemistry ; Rats ; Rats, Sprague-Dawley ; Receptors, GABA-B/analysis ; Receptors, GABA-B/chemistry ; Receptors, GABA-B/immunology
    Chemical Substances Antibodies ; Receptors, GABA-B
    Language English
    Publishing date 2000-04
    Publishing country France
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 645180-9
    ISSN 1460-9568 ; 0953-816X
    ISSN (online) 1460-9568
    ISSN 0953-816X
    DOI 10.1046/j.1460-9568.2000.01060.x
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article: Normal levels of anticoagulant heparan sulfate are not essential for normal hemostasis.

    HajMohammadi, Sassan / Enjyoji, Keiichi / Princivalle, Marc / Christi, Patricia / Lech, Miroslav / Beeler, David / Rayburn, Helen / Schwartz, John J / Barzegar, Samad / de Agostini, Ariane I / Post, Mark J / Rosenberg, Robert D / Shworak, Nicholas W

    The Journal of clinical investigation

    2003  Volume 111, Issue 7, Page(s) 989–999

    Abstract: Endothelial cell production of anticoagulant heparan sulfate (HS(act)) is controlled by the Hs3st1 gene, which encodes the rate-limiting enzyme heparan sulfate 3-O-sulfotransferase-1 (3-OST-1). In vitro, HS(act) dramatically enhances the neutralization ... ...

    Abstract Endothelial cell production of anticoagulant heparan sulfate (HS(act)) is controlled by the Hs3st1 gene, which encodes the rate-limiting enzyme heparan sulfate 3-O-sulfotransferase-1 (3-OST-1). In vitro, HS(act) dramatically enhances the neutralization of coagulation proteases by antithrombin. The in vivo role of HS(act) was evaluated by generating Hs3st1(-/-) knockout mice. Hs3st1(-/-) animals were devoid of 3-OST-1 enzyme activity in plasma and tissue extracts. Nulls showed dramatic reductions in tissue levels of HS(act) but maintained wild-type levels of tissue fibrin accumulation under both normoxic and hypoxic conditions. Given that vascular HS(act) predominantly occurs in the subendothelial matrix, mice were subjected to a carotid artery injury assay in which ferric chloride administration induces de-endothelialization and occlusive thrombosis. Hs3st1(-/-) and Hs3st1(+/+) mice yielded indistinguishable occlusion times and comparable levels of thrombin.antithrombin complexes. Thus, Hs3st1(-/-) mice did not show an obvious procoagulant phenotype. Instead, Hs3st1(-/-) mice exhibited genetic background-specific lethality and intrauterine growth retardation, without evidence of a gross coagulopathy. Our results demonstrate that the 3-OST-1 enzyme produces the majority of tissue HS(act). Surprisingly, this bulk of HS(act) is not essential for normal hemostasis in mice. Instead, 3-OST-1-deficient mice exhibited unanticipated phenotypes suggesting that HS(act) or additional 3-OST-1-derived structures may serve alternate biologic roles.
    MeSH term(s) Animals ; Anticoagulants/pharmacology ; Antithrombins/metabolism ; Carotid Arteries/pathology ; Crosses, Genetic ; Endothelium, Vascular/cytology ; Endothelium, Vascular/enzymology ; Exons ; Female ; Genotype ; Hemostasis ; Heparitin Sulfate/pharmacology ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Mice, Transgenic ; Models, Genetic ; Retroviridae/genetics ; Sulfotransferases/metabolism ; Thrombin/metabolism ; Tissue Distribution
    Chemical Substances Anticoagulants ; Antithrombins ; Heparitin Sulfate (9050-30-0) ; Sulfotransferases (EC 2.8.2.-) ; heparan sulfate D-glucosaminyl 3-O-sulfotransferase (EC 2.8.2.-) ; Thrombin (EC 3.4.21.5)
    Language English
    Publishing date 2003-04-03
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, U.S. Gov't, P.H.S.
    ZDB-ID 3067-3
    ISSN 1558-8238 ; 0021-9738
    ISSN (online) 1558-8238
    ISSN 0021-9738
    DOI 10.1172/JCI15809
    Database MEDical Literature Analysis and Retrieval System OnLINE

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