LIVIVO - Search results -

Search results

Result 1 - 10 of total 4406

Search options

Article ; Online: A Novel Strain-Specific Neutralizing Epitope on Glycoprotein H of Human Cytomegalovirus.

Thomas, Marco / Kropff, Barbara / Schneider, Andrea / Winkler, Thomas H / Görzer, Irene / Sticht, Heinrich / Britt, William J / Mach, Michael / Reuter, Nina

Journal of virology

2021 Volume 95, Issue 18, Page(s) e0065721

Abstract: Human cytomegalovirus (HCMV) is a ubiquitous pathogen that causes severe clinical disease in immunosuppressed patients and congenitally infected newborn infants. Viral envelope glycoproteins represent attractive targets for vaccination or passive ... ...

Abstract	Human cytomegalovirus (HCMV) is a ubiquitous pathogen that causes severe clinical disease in immunosuppressed patients and congenitally infected newborn infants. Viral envelope glycoproteins represent attractive targets for vaccination or passive immunotherapy. To extend the knowledge of mechanisms of virus neutralization, monoclonal antibodies (MAbs) were generated following immunization of mice with HCMV virions. Hybridoma supernatants were screened for
MeSH term(s)	Animals ; Antibodies, Monoclonal/immunology ; Antibodies, Neutralizing/immunology ; Antibodies, Viral/immunology ; Cytomegalovirus/classification ; Cytomegalovirus/immunology ; Cytomegalovirus Infections/immunology ; Cytomegalovirus Infections/virology ; Epitopes/immunology ; HEK293 Cells ; Humans ; Mice ; Mice, Inbred BALB C ; Viral Envelope Proteins/immunology
Chemical Substances	Antibodies, Monoclonal ; Antibodies, Neutralizing ; Antibodies, Viral ; Epitopes ; Viral Envelope Proteins ; glycoprotein H, Human cytomegalovirus
Language	English
Publishing date	2021-08-25
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	80174-4
ISSN	1098-5514 ; 0022-538X
ISSN (online)	1098-5514
ISSN	0022-538X
DOI	10.1128/JVI.00657-21
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 609: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Immobility in the sedentary plant-parasitic nematode H. glycines is associated with remodeling of neuromuscular tissue.

Han, Ziduan / Thapa, Sita / Reuter-Carlson, Ursula / Reed, Hannah / Gates, Michael / Lambert, Kris N / Schroeder, Nathan E

PLoS pathogens

2018 Volume 14, Issue 8, Page(s) e1007198

Abstract: ... muscle atrophy and loss of attachment to the underlying cuticle during immobile developmental stages. Male H ... of immunohistochemistry and molecular biology to characterize the GABAergic nervous system of H. glycines during mobile ... and immobile stages. We cloned and confirmed the function of the putative H. glycines GABA synthesis ...

Abstract	The sedentary plant-parasitic nematodes are considered among the most economically damaging pathogens of plants. Following infection and the establishment of a feeding site, sedentary nematodes become immobile. Loss of mobility is reversed in adult males while females never regain mobility. The structural basis for this change in mobility is unknown. We used a combination of light and transmission electron microscopy to demonstrate cell-specific muscle atrophy and sex-specific renewal of neuromuscular tissue in the sedentary nematode Heterodera glycines. We found that both females and males undergo body wall muscle atrophy and loss of attachment to the underlying cuticle during immobile developmental stages. Male H. glycines undergo somatic muscle renewal prior to molting into a mobile adult. In addition, we found developmental changes to the organization and number of motor neurons in the ventral nerve cord correlated with changes in mobility. To further examine neuronal changes associated with immobility, we used a combination of immunohistochemistry and molecular biology to characterize the GABAergic nervous system of H. glycines during mobile and immobile stages. We cloned and confirmed the function of the putative H. glycines GABA synthesis-encoding gene hg-unc-25 using heterologous rescue in C. elegans. We found a reduction in gene expression of hg-unc-25 as well as a reduction in the number of GABA-immunoreactive neurons during immobile developmental stages. Finally, we found evidence of similar muscle atrophy in the phylogenetically diverged plant-parasitic nematode Meloidogyne incognita. Together, our data demonstrate remodeling of neuromuscular structure and function during sedentary plant-parasitic nematode development.
MeSH term(s)	Animals ; Host-Parasite Interactions/physiology ; Movement/physiology ; Muscle, Skeletal/physiology ; Neurons/physiology ; Plant Diseases/parasitology ; Tylenchoidea/physiology
Language	English
Publishing date	2018-08-16
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	2205412-1
ISSN	1553-7374 ; 1553-7374
ISSN (online)	1553-7374
ISSN	1553-7374
DOI	10.1371/journal.ppat.1007198
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: The major autoantibody epitope on factor H in atypical hemolytic uremic syndrome is structurally different from its homologous site in factor H-related protein 1, supporting a novel model for induction of autoimmunity in this disease.

Bhattacharjee, Arnab / Reuter, Stefanie / Trojnár, Eszter / Kolodziejczyk, Robert / Seeberger, Harald / Hyvärinen, Satu / Uzonyi, Barbara / Szilágyi, Ágnes / Prohászka, Zoltán / Goldman, Adrian / Józsi, Mihály / Jokiranta, T Sakari

The Journal of biological chemistry

2015 Volume 290, Issue 15, Page(s) 9500–9510

Abstract: ... due to mutations in complement proteins or autoantibodies against complement factor H (CFH). It is ...

Abstract	Atypical hemolytic uremic syndrome (aHUS) is characterized by complement attack against host cells due to mutations in complement proteins or autoantibodies against complement factor H (CFH). It is unknown why nearly all patients with autoimmune aHUS lack CFHR1 (CFH-related protein-1). These patients have autoantibodies against CFH domains 19 and 20 (CFH19-20), which are nearly identical to CFHR1 domains 4 and 5 (CFHR14-5). Here, binding site mapping of autoantibodies from 17 patients using mutant CFH19-20 constructs revealed an autoantibody epitope cluster within a loop on domain 20, next to the two buried residues that are different in CFH19-20 and CFHR14-5. The crystal structure of CFHR14-5 revealed a difference in conformation of the autoantigenic loop in the C-terminal domains of CFH and CFHR1, explaining the variation in binding of autoantibodies from some aHUS patients to CFH19-20 and CFHR14-5. The autoantigenic loop on CFH seems to be generally flexible, as its conformation in previously published structures of CFH19-20 bound to the microbial protein OspE and a sialic acid glycan is somewhat altered. Cumulatively, our data suggest that association of CFHR1 deficiency with autoimmune aHUS could be due to the structural difference between CFHR1 and the autoantigenic CFH epitope, suggesting a novel explanation for CFHR1 deficiency in the pathogenesis of autoimmune aHUS.
MeSH term(s)	Atypical Hemolytic Uremic Syndrome/genetics ; Atypical Hemolytic Uremic Syndrome/immunology ; Atypical Hemolytic Uremic Syndrome/metabolism ; Autoantibodies/chemistry ; Autoantibodies/immunology ; Autoimmunity/genetics ; Autoimmunity/immunology ; Binding Sites/genetics ; Binding Sites/immunology ; Complement C3b Inactivator Proteins/chemistry ; Complement C3b Inactivator Proteins/genetics ; Complement C3b Inactivator Proteins/immunology ; Complement Factor H/chemistry ; Complement Factor H/genetics ; Complement Factor H/immunology ; Crystallography, X-Ray ; Epitope Mapping ; Epitopes/chemistry ; Epitopes/immunology ; Humans ; Models, Molecular ; Mutation ; Peptide Fragments/chemistry ; Peptide Fragments/immunology ; Protein Binding/immunology ; Protein Structure, Tertiary
Chemical Substances	Autoantibodies ; CFHR1 protein, human ; Complement C3b Inactivator Proteins ; Epitopes ; Peptide Fragments ; Complement Factor H (80295-65-4)
Language	English
Publishing date	2015-02-06
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2997-x
ISSN	1083-351X ; 0021-9258
ISSN (online)	1083-351X
ISSN	0021-9258
DOI	10.1074/jbc.M114.630871
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Uc I Zs.108: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Lewis-Brønsted Acid Pairs in Ga/H-ZSM-5 To Catalyze Dehydrogenation of Light Alkanes.

Schreiber, Moritz W / Plaisance, Craig P / Baumgärtl, Martin / Reuter, Karsten / Jentys, Andreas / Bermejo-Deval, Ricardo / Lercher, Johannes A

Journal of the American Chemical Society

2018 Volume 140, Issue 14, Page(s) 4849–4859

Abstract: The active sites for propane dehydrogenation in Ga/H-ZSM-5 with moderate concentrations ...

Abstract	The active sites for propane dehydrogenation in Ga/H-ZSM-5 with moderate concentrations of tetrahedral aluminum in the lattice were identified to be Lewis-Brønsted acid pairs. With increasing availability, Ga
Language	English
Publishing date	2018--11
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	3155-0
ISSN	1520-5126 ; 0002-7863
ISSN (online)	1520-5126
ISSN	0002-7863
DOI	10.1021/jacs.7b12901
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Bonn / Germany

Z 4' 55/32: Show issues
Z 7.3: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- See ZB MED holdings
- Order with fees

Article: Climate change drives trait-shifts in coral reef communities

Reuter, Hauke

Scientific reports, 9:3721

2019

Abstract: Climate change is expected to have profound, partly unforeseeable effects on the composition of functional traits of complex ecosystems, such as coral reefs, and some ecosystem properties are at risk of disappearing. This study applies a novel spatially ... ...

Institution	Leibniz-Zentrum für Marine Tropenforschung
Abstract	Climate change is expected to have profound, partly unforeseeable effects on the composition of functional traits of complex ecosystems, such as coral reefs, and some ecosystem properties are at risk of disappearing. This study applies a novel spatially explicit, individual-based model to explore three critical life history traits of corals: heat tolerance, competitiveness and growth performance under various environmental settings. Building upon these findings, we test the adaptation potential required by a coral community in order to not only survive but also retain its diversity by the end of this century under different IPCC climate scenarios. Even under the most favourable IPCC scenario (Representative Concentration Pathway, RCP 2.6), model results indicate that shifts in the trait space are likely and coral communities will mainly consist of small numbers of temperature-tolerant and fast-growing species. Species composition of coral communities is likely to be determined by heat tolerance, with competitiveness most likely playing a subordinate role. To sustain ~15% of current coral cover under a 2 °C temperature increase by the end of the century (RCP 4.5), coral systems would have to accommodate temperature increases of 0.1–0.15 °C per decade, assuming that periodic extreme thermal events occurred every 8 years. These required adaptation rates are unprecedented and unlikely, given corals’ life-history characteristics.
Keywords	Climate-change ecology ; Ecological modelling
Language	English
Document type	Article
Database	Repository for Life Sciences

Full text online

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Conference proceedings: Extrakte von H.pylori verhindern die Entwicklung einer allergischen Atemwegsentzündung über einen IL-10 und CD103+ cDC abhängigen Mechanismus

Reuter, S / Engler, D / Meyer-Martin, H / Maxeiner, J / Buhl, R / Müller, A / Taube, C

Pneumologie

2015

Abstract: ... wie sie unter anderem von Helicobacter pylori H. pylori ) induziert wird, auch eine wichtige Rolle bei der Suppression von Allergien spielt ... Studie wurde untersucht, ob mittels H. pylori Extrakt, vergleichbar mit der Lebendinfektion ... Lebenstag enteral mit H. pylori Extrakt behandelt. Mit 8 Wochen wurde bei den Tieren ein OVA abhängiges ...

Event/congress	Herbsttagung der Sektionen Zellbiologie, Pathophysiologie/Aerosolmedizin sowie Infektiologie und Tuberkulose in der Deutschen Gesellschaft für Pneumologie und Beatmungsmedizin e.V., Lübeck, 2014
Abstract	Die natürliche indigene Mikrobiota besteht aus mehreren tausend Bakterien. Im Vergleich zu Pathogenen können diese Bakterien im Körper überleben, da sie bereits in der Kindheit eine schützende Toleranz induzieren. Studien der letzten Jahre haben gezeigt, dass diese Toleranz, wie sie unter anderem von Helicobacter pylori H. pylori ) induziert wird, auch eine wichtige Rolle bei der Suppression von Allergien spielt. Epidemiologische Studien, aber auch eigene Untersuchungen im Tiermodell zeigen, dass Infektionen eine protektive Wirkung auf die Entstehung von Allergien, wie dem Asthma bronchiale haben. Dieser Schutz ist von Faktoren des Bakteriums abhängig und wird über eine DC und IL-18 vermittelte Generierung von Tregs induziert. In der vorliegenden Studie wurde untersucht, ob mittels H. pylori Extrakt, vergleichbar mit der Lebendinfektion, die Entwicklung einer allergischen Atemwegserkrankung unterdrückt werden kann. Hierzu wurden Mäuse ab dem 7. Lebenstag enteral mit H. pylori Extrakt behandelt. Mit 8 Wochen wurde bei den Tieren ein OVA abhängiges Modell der allergischen Atemwegsentzündung induziert. Im Vergleich zu unbehandelten Tieren entwickelten, Mäuse die mit dem Extrakt behandelt waren, eine abgemilderte allergische Atemwegsentzündung. In den mit H. pylori behandelten Tieren war eine deutliche Reduktion der Atemwegsüberempfindlichkeit, Anzahl der eosinophilen Granulozyten in der BAL, Th2 Zytokin Produktion, Entzündung im Lungengewebe sowie die Anzahl an PAS positiven Zellen Becherzellmetaplasie in der Lunge nachweisbar. Unter Einsatz von anti-IL10 Rezeptor Antikörpern und Tieren mit einer genetischen Deletion von Batf3, konnten wir evaluieren, dass sowohl die CD103 DC Subpopulation als auch IL-10 für den geschützten Phänotyp verantwortlich sind. Diese Daten verdeutlichen, dass H. pylori induzierte Immunescape-Mechanismen verwendet werden können um die Entwicklung von Allergien, wie Asthma bronchiale zu suppremieren. Dieses ist auch mit einem Extrakt der Bakterien möglich und bedarf keiner Lebendinfektion.
Language	German
Publishing date	2015-04-08
Publishing place	Stuttgart ; New York
Document type	Article ; Conference proceedings
ZDB-ID	607630-0
ISSN	1438-8790 ; 0934-8387
ISSN (online)	1438-8790
ISSN	0934-8387
DOI	10.1055/s-0035-1548657
Database	Thieme publisher's database

In stock of ZB MED Cologne/Königswinter

Uh II Zs.142: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular ab Jg. 2022: Lesesaal (EG)
Zs.MO 54: Show issues

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Lewis–Brønsted Acid Pairs in Ga/H-ZSM-5 To Catalyze Dehydrogenation of Light Alkanes

Schreiber, Moritz W / Andreas Jentys / Craig P. Plaisance / Johannes A. Lercher / Karsten Reuter / Martin Baumgärtl / Ricardo Bermejo-Deval

Journal of the American Chemical Society. 2018 Feb. 28, v. 140, no. 14

2018

Abstract: The active sites for propane dehydrogenation in Ga/H-ZSM-5 with moderate concentrations ... of magnitude higher than with the parent H-ZSM-5, highlighting the extraordinary activity of the Lewis–Brønsted ... that proceeds via heterolytic activation of the propane C–H bond followed by a monomolecular elimination of H2 ...

Abstract	The active sites for propane dehydrogenation in Ga/H-ZSM-5 with moderate concentrations of tetrahedral aluminum in the lattice were identified to be Lewis–Brønsted acid pairs. With increasing availability, Ga+ and Brønsted acid site concentrations changed inversely, as protons of Brønsted acid sites were exchanged with Ga+. At a Ga/Al ratio of 1/2, the rate of propane dehydrogenation was 2 orders of magnitude higher than with the parent H-ZSM-5, highlighting the extraordinary activity of the Lewis–Brønsted acid pairs. Density functional theory calculations relate the high activity to a bifunctional mechanism that proceeds via heterolytic activation of the propane C–H bond followed by a monomolecular elimination of H2 and desorption of propene.
Keywords	active sites ; aluminum ; Bronsted acids ; chemical bonding ; dehydrogenation ; density functional theory ; desorption ; gallium ; heterolytic cleavage ; hydrogen ; propane ; propylene ; protons
Language	English
Dates of publication	2018-0228
Size	p. 4849-4859.
Publishing place	American Chemical Society
Document type	Article
ZDB-ID	3155-0
ISSN	1520-5126 ; 0002-7863
ISSN (online)	1520-5126
ISSN	0002-7863
DOI	10.1021/jacs.7b12901
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Bonn / Germany

Z 4' 55/32: Show issues
Z 7.3: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Immobility in the sedentary plant-parasitic nematode H. glycines is associated with remodeling of neuromuscular tissue.

Ziduan Han / Sita Thapa / Ursula Reuter-Carlson / Hannah Reed / Michael Gates / Kris N Lambert / Nathan E Schroeder

PLoS Pathogens, Vol 14, Iss 8, p e

2018 Volume 1007198

Abstract	The sedentary plant-parasitic nematodes are considered among the most economically damaging pathogens of plants. Following infection and the establishment of a feeding site, sedentary nematodes become immobile. Loss of mobility is reversed in adult males while females never regain mobility. The structural basis for this change in mobility is unknown. We used a combination of light and transmission electron microscopy to demonstrate cell-specific muscle atrophy and sex-specific renewal of neuromuscular tissue in the sedentary nematode Heterodera glycines. We found that both females and males undergo body wall muscle atrophy and loss of attachment to the underlying cuticle during immobile developmental stages. Male H. glycines undergo somatic muscle renewal prior to molting into a mobile adult. In addition, we found developmental changes to the organization and number of motor neurons in the ventral nerve cord correlated with changes in mobility. To further examine neuronal changes associated with immobility, we used a combination of immunohistochemistry and molecular biology to characterize the GABAergic nervous system of H. glycines during mobile and immobile stages. We cloned and confirmed the function of the putative H. glycines GABA synthesis-encoding gene hg-unc-25 using heterologous rescue in C. elegans. We found a reduction in gene expression of hg-unc-25 as well as a reduction in the number of GABA-immunoreactive neurons during immobile developmental stages. Finally, we found evidence of similar muscle atrophy in the phylogenetically diverged plant-parasitic nematode Meloidogyne incognita. Together, our data demonstrate remodeling of neuromuscular structure and function during sedentary plant-parasitic nematode development.
Keywords	Immunologic diseases. Allergy ; RC581-607 ; Biology (General) ; QH301-705.5
Subject code	571
Language	English
Publishing date	2018-08-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article: Depolarization-induced changes of free intracellular Ca(2+) concentration and of [(3)H]dopamine release in undifferentiated and differentiated PC12 cells.

Reber, B F / Porzig, H / Becker, C / Reuter, H

Neurochemistry international

2009 Volume 17, Issue 2, Page(s) 197–203

Abstract: ... measured with fura-2 and on [(3)H]dopamine release. These drugs act on different types of voltage-gated Ca ... undifferentiated cells. Concomitantly we saw a shift from dihydropyridine-sensitive [(3)H]dopamine release ... of Ca channels are predominantly involved in [(3)H]dopamine release in undifferentiated and ...

Abstract	The rat pheochromocytoma cell line PC12 undergoes morphological differentiation into neurone-like cells when exposed to nerve growth factor (NGF). We found that in PC12 cells the enzymatic activity of choline-acetyltransferase and of tyrosine hydroxylase changed very little during NGF exposure, while tyrosine hydroxylase activity increased during other treatments of the cells. These enzymes are rate limiting for the synthesis of cholinergic and adrenergic neurotransmitters, respectively. In order to learn more about mechanisms involved in catecholamine release from differentiating PC12 cells, we studied the effects of dihydropyridines and of ?-conotoxin on K(+)-depolarization-induced [Ca(2+)](i) transients measured with fura-2 and on [(3)H]dopamine release. These drugs act on different types of voltage-gated Ca channels. We found that in differentiated cells [Ca(2+)](i) transients in growth cones are more sensitive to ?-conotoxin than to dihydropyridines, while the opposite is true for cell bodies in both differentiated and undifferentiated cells. Concomitantly we saw a shift from dihydropyridine-sensitive [(3)H]dopamine release in undifferentiated cells to ?-conotoxin-sensitive release in differentiated cells. We conclude that different types of Ca channels are predominantly involved in [(3)H]dopamine release in undifferentiated and differentiated PC12 cells, respectively.
Language	English
Publishing date	2009-11-16
Publishing country	England
Document type	Journal Article
ZDB-ID	283190-9
ISSN	1872-9754 ; 0197-0186
ISSN (online)	1872-9754
ISSN	0197-0186
DOI	10.1016/0197-0186(90)90142-g
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1610: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: An engineered construct combining complement regulatory and surface-recognition domains represents a minimal-size functional factor H.

Hebecker, Mario / Alba-Domínguez, María / Roumenina, Lubka T / Reuter, Stefanie / Hyvärinen, Satu / Dragon-Durey, Marie-Agnès / Jokiranta, T Sakari / Sánchez-Corral, Pilar / Józsi, Mihály

Journal of immunology (Baltimore, Md. : 1950)

2013 Volume 191, Issue 2, Page(s) 912–921

Abstract: ... activation leads to pathology. Polymorphisms, mutations, and autoantibodies affecting factor H (FH), a major ...

Abstract	Complement is an essential humoral component of innate immunity; however, its inappropriate activation leads to pathology. Polymorphisms, mutations, and autoantibodies affecting factor H (FH), a major regulator of the alternative complement pathway, are associated with various diseases, including age-related macular degeneration, atypical hemolytic uremic syndrome, and C3 glomerulopathies. Restoring FH function could be a treatment option for such pathologies. In this article, we report on an engineered FH construct that directly combines the two major functional regions of FH: the N-terminal complement regulatory domains and the C-terminal surface-recognition domains. This minimal-size FH (mini-FH) binds C3b and has complement regulatory functions similar to those of the full-length protein. In addition, we demonstrate that mini-FH binds to the FH ligands C-reactive protein, pentraxin 3, and malondialdehyde epitopes. Mini-FH was functionally active when bound to the extracellular matrix and endothelial cells in vitro, and it inhibited C3 deposition on the cells. Furthermore, mini-FH efficiently inhibited complement-mediated lysis of host-like cells caused by a disease-associated FH mutation or by anti-FH autoantibodies. Therefore, mini-FH could potentially be used as a complement inhibitor targeting host surfaces, as well as to replace compromised FH in diseases associated with FH dysfunction.
MeSH term(s)	Amino Acid Sequence ; Base Sequence ; C-Reactive Protein/metabolism ; Complement Activation ; Complement C3b/antagonists & inhibitors ; Complement C3b/immunology ; Complement C3b/metabolism ; Complement Factor H/genetics ; Complement Factor H/immunology ; Complement Pathway, Alternative ; Endothelial Cells/metabolism ; Extracellular Matrix/metabolism ; Humans ; Malondialdehyde/metabolism ; Protein Binding ; Protein Engineering ; Recombinant Proteins/immunology ; Recombinant Proteins/metabolism ; Sequence Analysis, DNA ; Serum Amyloid P-Component/metabolism
Chemical Substances	Recombinant Proteins ; Serum Amyloid P-Component ; PTX3 protein (148591-49-5) ; Malondialdehyde (4Y8F71G49Q) ; Complement C3b (80295-43-8) ; Complement Factor H (80295-65-4) ; C-Reactive Protein (9007-41-4)
Language	English
Publishing date	2013-07-15
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	3056-9
ISSN	1550-6606 ; 0022-1767 ; 1048-3233 ; 1047-7381
ISSN (online)	1550-6606
ISSN	0022-1767 ; 1048-3233 ; 1047-7381
DOI	10.4049/jimmunol.1300269
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Ud II Zs.37: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular ab Jg. 2022: Lesesaal (EG)
Zs.MG 28: Show issues

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

To top

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito