Article ; Online: An Indirect Action Contributes to C-Fos Induction in Paraventricular Hypothalamic Nucleus by Neuropeptide Y.
2016 Volume 6, Page(s) 19980
Abstract: Neuropeptide Y (NPY) is a well-established orexigenic peptide and hypothalamic ... expression of C-Fos, an indicator for neuronal activation, in the PVH, which has been used extensively ... to examine the underlying NPY orexigenic neural pathways. However, PVH C-Fos induction is in discordance ...
| Abstract | Neuropeptide Y (NPY) is a well-established orexigenic peptide and hypothalamic paraventricular nucleus (PVH) is one major brain site that mediates the orexigenic action of NPY. NPY induces abundant expression of C-Fos, an indicator for neuronal activation, in the PVH, which has been used extensively to examine the underlying NPY orexigenic neural pathways. However, PVH C-Fos induction is in discordance with the abundant expression of NPY receptors, a group of inhibitory Gi protein coupled receptors in the PVH, and with the overall role of PVH neurons in feeding inhibition, suggesting a mechanism of indirect action. Here we showed that the ability of NPY on C-Fos induction in the PVH was blunted in conditions of insulin deficiency and fasting, a condition associated with a high level of NPY and a low level of insulin. Moreover, insulin insufficiency blunted C-Fos induction in the PVH by fasting-induced re-feeding, and insulin and NPY induced c-Fos induction in the same group of PVH neurons. Finally, NPY produced normal C-Fos induction in the PVH with disruption of GABA-A receptors. Thus, our results revealed that PVH C-Fos induction by NPY is mediated by an indirect action, which is at least partially mediated by insulin action, but not GABA-A receptors. |
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| MeSH term(s) | Animals ; Diabetes Mellitus, Experimental ; Diabetes Mellitus, Type 1 ; Fasting ; GABAergic Neurons/drug effects ; GABAergic Neurons/metabolism ; Gene Expression ; Insulin/deficiency ; Insulin/genetics ; Insulin/metabolism ; Male ; Mice ; Mice, Transgenic ; Neuropeptide Y/metabolism ; Neuropeptide Y/pharmacology ; Paraventricular Hypothalamic Nucleus/drug effects ; Paraventricular Hypothalamic Nucleus/metabolism ; Proto-Oncogene Proteins c-fos/genetics ; Proto-Oncogene Proteins c-fos/metabolism ; Receptors, GABA-A/metabolism |
| Chemical Substances | Insulin ; Neuropeptide Y ; Proto-Oncogene Proteins c-fos ; Receptors, GABA-A |
| Language | English |
| Publishing date | 2016-01-27 |
| Publishing country | England |
| Document type | Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 2615211-3 |
| ISSN | 2045-2322 ; 2045-2322 |
| ISSN (online) | 2045-2322 |
| ISSN | 2045-2322 |
| DOI | 10.1038/srep19980 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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