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Article: 25-Hydroxycholecalciferol Inhibits Cell Growth and Induces Apoptosis in SiHa Cervical Cells via Autocrine Vitamin D Metabolism.

Punchoo, Rivak / Dreyer, Greta / Pillay, Tahir S

2023 Volume 11, Issue 3

Abstract: Preclinical studies show that the anticancer actions of vitamin D metabolites are mediated ... express an autocrine vitamin D metabolising system (VDMS) comprised of a vitamin D receptor, vitamin D ...

Abstract	Preclinical studies show that the anticancer actions of vitamin D metabolites are mediated by apoptosis, inhibition of cell proliferation and induction of cell cycle arrest. Cervical cancer cells express an autocrine vitamin D metabolising system (VDMS) comprised of a vitamin D receptor, vitamin D catabolic enzyme (CYP24A1), and the activating enzyme of 25-hydroxycholecalciferol (25(OH)D3), CYP27B1. We assessed the anticancer effects of 25(OH)D3 at clinically relevant concentrations on a cervical squamous cell cancer cell line, SiHa. We evaluated cell health parameters (cell count, viability, and cell cycle), cell death modes (apoptosis, autophagic-dependent death, and necrosis by flow cytometry and transmission electron microscopy), and autocrine VDMS gene and protein expression by qPCR and Western blot, respectively. Our study demonstrates that physiological and supraphysiological doses of 25(OH)D3 inhibit cell growth and viability and induce biochemical and morphological apoptosis in SiHa cells. These growth effects are mediated by alteration in the VDMS gene and protein expression, with prominent negative feedback at supraphysiological treatment dose. These data identify promising therapeutic potential of 25(OH)D3 in cervical cancer, which warrants further clinical translational investigations.
Language	English
Publishing date	2023-03-13
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2720867-9
ISSN	2227-9059
ISSN	2227-9059
DOI	10.3390/biomedicines11030871
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Book ; Online: Project D

Abass, A. / Abele, S. / Andrade, M. / Asten, Piet J.A. van / Coyne, Danny L. / Dochez, C. / Dubois, T. / Gerguson, M. / Fermont, A. / Ferris, S. / Gold, C. / Hanna, R. / Khizzah, B. / Kanju, E. / Kolijn, S. / Legg, James P. / Mahungu, N. / Manyong, V. / Muchopa, C. /

Nankinga, C. / Niere, B. / Ntawuruhungu, P. / Okech, S. / Pillay, M. / Ragama, P. / Tenkouano, A. / Toko, M. / Wendt, J. / Whyte, J.

promoting food security and income generation through sustainable production and commercialization of starchy and grain staples in eastern and southern Africa

2018

Keywords	food security ; livelihoods ; socioeconic development ; eastern africa
Language	English
Publishing date	2018-08-09T06:44:11Z
Publishing country	fr
Document type	Book ; Online
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Article ; Online: 25-Hydroxycholecalciferol Inhibits Cell Growth and Induces Apoptosis in SiHa Cervical Cells via Autocrine Vitamin D Metabolism

Rivak Punchoo / Greta Dreyer / Tahir S. Pillay

Biomedicines, Vol 11, Iss 871, p

2023 Volume 871

Abstract	Preclinical studies show that the anticancer actions of vitamin D metabolites are mediated by apoptosis, inhibition of cell proliferation and induction of cell cycle arrest. Cervical cancer cells express an autocrine vitamin D metabolising system (VDMS) comprised of a vitamin D receptor, vitamin D catabolic enzyme (CYP24A1), and the activating enzyme of 25-hydroxycholecalciferol (25(OH)D3), CYP27B1. We assessed the anticancer effects of 25(OH)D3 at clinically relevant concentrations on a cervical squamous cell cancer cell line, SiHa. We evaluated cell health parameters (cell count, viability, and cell cycle), cell death modes (apoptosis, autophagic-dependent death, and necrosis by flow cytometry and transmission electron microscopy), and autocrine VDMS gene and protein expression by qPCR and Western blot, respectively. Our study demonstrates that physiological and supraphysiological doses of 25(OH)D3 inhibit cell growth and viability and induce biochemical and morphological apoptosis in SiHa cells. These growth effects are mediated by alteration in the VDMS gene and protein expression, with prominent negative feedback at supraphysiological treatment dose. These data identify promising therapeutic potential of 25(OH)D3 in cervical cancer, which warrants further clinical translational investigations.
Keywords	cervical cancer ; SiHa ; 25-hydroxycholecalciferol ; apoptosis ; vitamin D metabolising system ; 24-hydroxylase (CYP24A1) ; Biology (General) ; QH301-705.5
Subject code	610
Language	English
Publishing date	2023-03-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
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Article ; Conference proceedings: Should 18F-FDG PET/CT Scans (2-Deoxy-2[fluorine 18] fluoro-D-glucose Integrated with Computed Tomography) Replace 99mTc-MDP (Methylene Diphosphonate) Bone Scintigraphy in the Management of Breast Cancer Patients with Suspected Skeletal Metastases?

Patel, Maryam / Hadebe, Bawanile / Pillay, Venesen / Harry, Lerwine

World Journal of Nuclear Medicine

2023 Volume 22, Issue 02

Event/congress	Abstracts for the 18th International Conference on Radiopharmaceutical Therapy (ICRT), Accra, Ghana, 2023-05-01
Language	English
Publishing date	2023-05-25
Publisher	Thieme Medical and Scientific Publishers Pvt. Ltd.
Publishing place	Stuttgart ; New York
Document type	Article ; Conference proceedings
ZDB-ID	2911903-0
ISSN	1607-3312 ; 1450-1147
ISSN (online)	1607-3312
ISSN	1450-1147
DOI	10.1055/s-0043-1769982
Database	Thieme publisher's database

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Article ; Online: Synthesis and characterization of fluorescent Europium (III) complex based on D-dextrose composite for latent fingerprint detection

Eswaran Prabakaran / Kriveshini Pillay

Journal of Saudi Chemical Society, Vol 24, Iss 8, Pp 584-

2020 Volume 605

Abstract: ... hexahydrate (EuCl3·6H2O) by a complexation method. The red emission fluorescent Na[Eu(5,5′-DMBP)(phen)3]·Cl3/D ... Dextrose (Eu(III)-CPLx/D-Dex) composite was synthesized by using an adsorption method with Eu(III)-CPLx and ... D-Dextrose (D-Dex). The Eu(III)-CPLx and fluorescent (Eu(III)-CPLx/D-Dex) composites were ...

Abstract	A europium salt-Na[Eu(5,5′-DMBP)(phen)3]·Cl3 (Eu(III)-CPLx) was prepared by using various precursors such as 5,5′-Dimethyl-2,2′-bipyridyl (5,5′-DMBP), 1,10-phenanthroline (phen) and europium chloride hexahydrate (EuCl3·6H2O) by a complexation method. The red emission fluorescent Na[Eu(5,5′-DMBP)(phen)3]·Cl3/D-Dextrose (Eu(III)-CPLx/D-Dex) composite was synthesized by using an adsorption method with Eu(III)-CPLx and D-Dextrose (D-Dex). The Eu(III)-CPLx and fluorescent (Eu(III)-CPLx/D-Dex) composites were characterized by numerous techniques. The fluorescent (Eu(III)-CPLx/D-Dex) composite demonstrated a strong red emission and controlled fluorescence quenching in the solid state and was consequently used in latent fingerprint (LFP) detection. The LFPs were developed by using a powder dusting method (PDM) with Eu(III)-CPLx and fluorescent Eu(III)-CPLx/D-Dex composites on different substrates under daylight and UV-light irradiation at 365 nm. The fluorescent Eu(III)-CPLx/D-Dex composite was effectively explored for developing LFP images on various substrates and also acts as a better labeling agent for LFP detection in forensic science crime scene investigations.
Keywords	Europium (III) complex ; Fluorescent Europium (III) complex/D-dextrose composite ; Latent fingerprint detection ; Powder dusting method ; Chemistry ; QD1-999
Subject code	540
Language	English
Publishing date	2020-08-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
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Article: Development and Mechanistic Insight into the Enhanced Cytotoxic Potential of Parvifloron D Albumin Nanoparticles in EGFR-Overexpressing Pancreatic Cancer Cells.

Santos-Rebelo, Ana / Kumar, Pradeep / Pillay, Viness / Choonara, Yahya E / Eleutério, Carla / Figueira, Mariana / Viana, Ana S / Ascensão, Lia / Molpeceres, Jesús / Rijo, Patrícia / Correia, Isabel / Amaral, Joana / Solá, Susana / Rodrigues, Cecília M P / Gaspar, Maria Manuela / Reis, Catarina Pinto

Cancers

2019 Volume 11, Issue 11

Abstract: Pancreatic cancer is one of the most lethal cancers, with an extremely poor prognosis. The development of more effective therapies is thus imperative. Natural origin compounds isolated ... ...

Abstract	Pancreatic cancer is one of the most lethal cancers, with an extremely poor prognosis. The development of more effective therapies is thus imperative. Natural origin compounds isolated from
Language	English
Publishing date	2019-11-05
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2527080-1
ISSN	2072-6694
ISSN	2072-6694
DOI	10.3390/cancers11111733
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Article ; Online: Vitamin A and D Deficiencies Associated With Incident Tuberculosis in HIV-Infected Patients Initiating Antiretroviral Therapy in Multinational Case-Cohort Study.

Journal of acquired immune deficiency syndromes (1999)

2017 Volume 75, Issue 3, Page(s) e71–e79

Abstract: ... A, carotenoids, vitamin B6, vitamin B12, vitamin D, vitamin E, and selenium. We measured associations between ... ratio, aHR 5.33, 95% confidence interval, CI: 1.54 to 18.43) and vitamin D (aHR 3.66, 95% CI: 1.16 to 11 ... from predominantly low- and middle-income countries, deficiencies in vitamin A and vitamin D at ART initiation were ...

Abstract	Introduction: Numerous micronutrients have immunomodulatory roles that may influence risk of tuberculosis (TB), but the association between baseline micronutrient deficiencies and incident TB after antiretroviral therapy (ART) initiation in HIV-infected individuals is not well characterized. Methods: We conducted a case-cohort study (n = 332) within a randomized trial comparing 3 ART regimens in 1571 HIV treatment-naive adults from 9 countries. A subcohort of 30 patients was randomly selected from each country (n = 270). Cases (n = 77; main cohort = 62, random subcohort = 15) included patients diagnosed with TB by 96 weeks post-ART initiation. We determined pretreatment concentrations of vitamin A, carotenoids, vitamin B6, vitamin B12, vitamin D, vitamin E, and selenium. We measured associations between pretreatment micronutrient deficiencies and incident TB using Breslow-weighted Cox regression models. Results: Median pretreatment CD4 T-cell count was 170 cells/mm; 47.3% were women; and 53.6% Black. In multivariable models after adjusting for age, sex, country, treatment arm, previous TB, baseline CD4 count, HIV viral load, body mass index, and C-reactive protein, pretreatment deficiency in vitamin A (adjusted hazard ratio, aHR 5.33, 95% confidence interval, CI: 1.54 to 18.43) and vitamin D (aHR 3.66, 95% CI: 1.16 to 11.51) were associated with TB post-ART. Conclusions: In a diverse cohort of HIV-infected adults from predominantly low- and middle-income countries, deficiencies in vitamin A and vitamin D at ART initiation were independently associated with increased risk of incident TB in the ensuing 96 weeks. Vitamin A and D may be important modifiable risk factors for TB in high-risk HIV-infected patients starting ART in resource-limited highly-TB-endemic settings.
MeSH term(s)	Adult ; Anti-Retroviral Agents/administration & dosage ; Anti-Retroviral Agents/adverse effects ; Antiretroviral Therapy, Highly Active/adverse effects ; CD4-Positive T-Lymphocytes ; Coinfection ; Female ; HIV Infections/blood ; HIV Infections/drug therapy ; HIV Infections/immunology ; Humans ; Male ; Malnutrition/blood ; Malnutrition/complications ; Micronutrients/blood ; Micronutrients/deficiency ; Prevalence ; Prospective Studies ; Tuberculosis/drug therapy ; Tuberculosis/immunology ; Vitamin A Deficiency/blood ; Vitamin A Deficiency/complications ; Vitamin A Deficiency/immunology ; Vitamin D Deficiency/blood ; Vitamin D Deficiency/complications ; Vitamin D Deficiency/immunology
Chemical Substances	Anti-Retroviral Agents ; Micronutrients
Language	English
Publishing date	2017-03-20
Publishing country	United States
Document type	Journal Article ; Multicenter Study ; Randomized Controlled Trial ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
ZDB-ID	645053-2
ISSN	1944-7884 ; 1077-9450 ; 0897-5965 ; 0894-9255 ; 1525-4135
ISSN (online)	1944-7884 ; 1077-9450
ISSN	0897-5965 ; 0894-9255 ; 1525-4135
DOI	10.1097/QAI.0000000000001308
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Article ; Online: Pre-analytical stability of 25(OH)-vitamin D in primary collection tubes.

Hayden, Yolandie / Pillay, Taryn / Marx, Gerda / de Lange, Wimpie / Kuyl, Johannes M

Clinical chemistry and laboratory medicine

2015 Volume 53, Issue 3, Page(s) e55–7

MeSH term(s)	Blood Specimen Collection ; Chromatography, High Pressure Liquid ; Drug Stability ; Gels/chemistry ; Humans ; Specimen Handling ; Vitamin D/analogs & derivatives ; Vitamin D/blood
Chemical Substances	Gels ; Vitamin D (1406-16-2) ; 25-hydroxyvitamin D (A288AR3C9H)
Language	English
Publishing date	2015-02
Publishing country	Germany
Document type	Letter
ZDB-ID	1418007-8
ISSN	1437-4331 ; 1434-6621 ; 1437-8523
ISSN (online)	1437-4331
ISSN	1434-6621 ; 1437-8523
DOI	10.1515/cclm-2014-0543
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Article ; Online: Relationship between vitamin D, calcium and parathyroid hormone in Cape Town.

Haarburger, D / Hoffman, M / Erasmus, R T / Pillay, T S

Journal of clinical pathology

2009 Volume 62, Issue 6, Page(s) 567–569

Abstract: Aim: The aim of this study was to test the hypothesis that vitamin D deficiency is associated ... with abnormal levels of calcium and parathyroid hormone (PTH).: Methods: Vitamin D requests ... Results: Only when the 25-hydroxyvitamin D (25(OH)D) level dropped below 25 nmol/l, was there ...

Abstract	Aim: The aim of this study was to test the hypothesis that vitamin D deficiency is associated with abnormal levels of calcium and parathyroid hormone (PTH). Methods: Vitamin D requests at a tertiary hospital in South Africa over 2 years were retrospectively analysed along with calcium and PTH levels. Results: Only when the 25-hydroxyvitamin D (25(OH)D) level dropped below 25 nmol/l, was there a significant rise in PTH. A subnormal 25(OH)D level was also not always related to hypocalcaemia, as more than half of patients with their 25(OH)D level below 25 nmol/l had calcium levels in the reference range. However, all patients with calcium levels below 1.8 mmol/l were shown to have vitamin D insufficiency. Conclusion: Hypovitaminosis D may co-exist with a blunted PTH response. Therefore, assumptions about vitamin D status should not be made based on PTH and calcium values. 25(OH)D measurements should be requested when vitamin D deficiency is clinically suspected, irrespective of biochemical results.
MeSH term(s)	Adult ; Biomarkers/blood ; Calcium/blood ; Child, Preschool ; Creatinine/blood ; Female ; Homeostasis ; Humans ; Hypocalcemia/blood ; Infant ; Male ; Middle Aged ; Parathyroid Hormone/blood ; Reference Values ; Retrospective Studies ; Rickets/blood ; Seasons ; South Africa ; Sunlight ; Vitamin D/analogs & derivatives ; Vitamin D/blood ; Vitamin D Deficiency/blood ; Vitamin D Deficiency/diagnosis
Chemical Substances	Biomarkers ; PTH protein, human ; Parathyroid Hormone ; Vitamin D (1406-16-2) ; 25-hydroxyvitamin D (A288AR3C9H) ; Creatinine (AYI8EX34EU) ; Calcium (SY7Q814VUP)
Language	English
Publishing date	2009-06
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	80261-x
ISSN	1472-4146 ; 0021-9746
ISSN (online)	1472-4146
ISSN	0021-9746
DOI	10.1136/jcp.2008.062877
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Article ; Online: Combating antimicrobial resistance in neonatal infections: a South African perspective.

Pillay, Dharshni

The Lancet. Global health

2022 Volume 10, Issue 8, Page(s) e1082–e1083

MeSH term(s)	Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/therapeutic use ; Communicable Diseases ; Cross Infection ; Drug Resistance, Bacterial ; Humans ; Infant, Newborn ; South Africa/epidemiology
Chemical Substances	Anti-Bacterial Agents
Language	English
Publishing date	2022-07-14
Publishing country	England
Document type	Journal Article ; Comment
ZDB-ID	2723488-5
ISSN	2214-109X ; 2214-109X
ISSN (online)	2214-109X
ISSN	2214-109X
DOI	10.1016/S2214-109X(22)00288-1
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