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Article ; Online: Extra-urogenital infection by Mycoplasma hominis in transplant patients: two case reports and literature review.

Ahamad, Afrinash / Zervou, Fainareti N / Aguero-Rosenfeld, Maria E

2023 Volume 23, Issue 1, Page(s) 601

Abstract: Background: Mycoplasma hominis is a facultative anaerobic bacterium commonly present in the urogenital tract. In recent years, M. hominis has increasingly been associated with extra-urogenital tract infections, particularly in immunosuppressed patients. ...

Abstract	Background: Mycoplasma hominis is a facultative anaerobic bacterium commonly present in the urogenital tract. In recent years, M. hominis has increasingly been associated with extra-urogenital tract infections, particularly in immunosuppressed patients. Detecting M. hominis in a diagnostic laboratory can be challenging due to its slow growth rate, absence of a cell wall, and the requirements of specialized media and conditions for optimal growth. Consequently, it is necessary to establish guidelines for the detection of this microorganism and to request the appropriate microbiological work-up of immunosuppressed patients. Case presentation: We hereby present two cases of solid organ transplant patients who developed M. hominis infection. Microscopic examination of the bronchial lavage and pleural fluid showed no microorganisms. However, upon inoculating the specimens onto routine microbiology media, the organism was successfully identified and confirmation was performed using 16S rDNA sequencing. Both patients received appropriate treatment resulting in the resolution of M. hominis infection. Conclusions: The prompt detection of M. hominis in a clinical specimen can have a significant impact on patient care by allowing for early intervention and ultimately resulting in more favorable clinical outcomes, especially in transplant patients.
MeSH term(s)	Humans ; Mycoplasma hominis ; Base Composition ; Phylogeny ; RNA, Ribosomal, 16S/genetics ; Sequence Analysis, DNA ; Urinary Tract Infections
Chemical Substances	RNA, Ribosomal, 16S
Language	English
Publishing date	2023-09-14
Publishing country	England
Document type	Review ; Case Reports ; Journal Article
ZDB-ID	2041550-3
ISSN	1471-2334 ; 1471-2334
ISSN (online)	1471-2334
ISSN	1471-2334
DOI	10.1186/s12879-023-08593-2
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Article ; Online: High antibody levels in cord blood from pregnant women vaccinated against COVID-19.

Trostle, Megan E / Aguero-Rosenfeld, Maria E / Roman, Ashley S / Lighter, Jennifer L

American journal of obstetrics & gynecology MFM

2021 Volume 3, Issue 6, Page(s) 100481

MeSH term(s)	COVID-19 ; Female ; Fetal Blood ; Humans ; Pregnancy ; Pregnant Women ; SARS-CoV-2 ; Vaccination
Language	English
Publishing date	2021-09-22
Publishing country	United States
Document type	Letter
ISSN	2589-9333
ISSN (online)	2589-9333
DOI	10.1016/j.ajogmf.2021.100481
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Article ; Online: Rate and consequences of missed Clostridioides (Clostridium) difficile infection diagnosis from nonreporting of Clostridioides difficile results of the multiplex GI PCR panel: experience from two-hospitals.

Zacharioudakis, Ioannis M / Zervou, Fainareti N / Phillips, Michael S / Aguero-Rosenfeld, Maria E

Diagnostic microbiology and infectious disease

2021 Volume 100, Issue 2, Page(s) 115346

Abstract: Introduction: It is common among microbiology laboratories to blind the Clostridioides difficile (C. difficile) BioFire FilmArray GI Panel result in fear of overdiagnosis.: Methods: We examined the rate of missed community-onset C. difficile ... ...

Abstract	Introduction: It is common among microbiology laboratories to blind the Clostridioides difficile (C. difficile) BioFire FilmArray GI Panel result in fear of overdiagnosis. Methods: We examined the rate of missed community-onset C. difficile infection (CDI) diagnosis and associated outcomes. Adult patients with FilmArray GI Panel positive for C. difficile on hospital admission who lacked dedicated C. difficile testing were included. Results: Among 144 adults with a FilmArray Panel positive for C. difficile, 18 did not have concurrent dedicated C. difficile testing. Eight patients were categorized as possible, 5 as probable and 4 as definite cases of missed CDI diagnosis. We observed associated delays in initiation of appropriate therapy, intensive care unit admissions, hospital readmissions, colorectal surgery and death/discharge to hospice. Five out of 17 lacked risk factors for CDI. Conclusion: The practice of concealing C. difficile FilmArray GI Panel results needs to be reconsidered in patients presenting with community-onset colitis.
MeSH term(s)	Adult ; Aged ; Aged, 80 and over ; Clostridioides difficile ; Clostridium Infections/diagnosis ; Clostridium Infections/microbiology ; Cohort Studies ; Female ; Hospitals ; Humans ; Male ; Middle Aged ; Multiplex Polymerase Chain Reaction/methods ; Retrospective Studies
Language	English
Publishing date	2021-02-12
Publishing country	United States
Document type	Journal Article
ZDB-ID	604920-5
ISSN	1879-0070 ; 0732-8893
ISSN (online)	1879-0070
ISSN	0732-8893
DOI	10.1016/j.diagmicrobio.2021.115346
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Zs.A 1845: Show issues

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Article ; Online: SARS-CoV-2 antibodies: IgA correlates with severity of disease in early COVID-19 infection.

Zervou, Fainareti N / Louie, Ping / Stachel, Anna / Zacharioudakis, Ioannis M / Ortiz-Mendez, Yadira / Thomas, Kristen / Aguero-Rosenfeld, Maria E

Journal of medical virology

2021 Volume 93, Issue 9, Page(s) 5409–5415

Abstract: Timing of detection of immunoglobulin G (IgG), immunoglobulin A (IgA), and immunoglobulin M (IgM) antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), and their use to support the diagnosis are of increasing interest. We used ... ...

Abstract	Timing of detection of immunoglobulin G (IgG), immunoglobulin A (IgA), and immunoglobulin M (IgM) antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), and their use to support the diagnosis are of increasing interest. We used the Gold Standard Diagnostics ELISA to evaluate the kinetics of SARS-CoV-2 IgG, IgA, and IgM antibodies in sera of 82 hospitalized patients with polymerase chain reaction (PCR)-confirmed coronavirus disease 2019 (COVID-19). Serum samples were collected 1-59 days post-onset of symptoms (PoS) and we examined the association of age, sex, disease severity, and symptoms' duration with antibody levels. We also tested sera of 100 ambulatory hospital employees with PCR-confirmed COVID-19 and samples collected during convalescence, 35-57 days PoS. All but four of the admitted patients (95.1%) developed antibodies to SARS-CoV-2. Antibodies were detected within 7 days PoS; IgA in 60.0%, IgM in 53.3%, and IgG in 46.7% of samples. IgG positivity increased to 100% on Day 21. We did not observe significant differences in the rate of antibody development in regard to age and sex. IgA levels were highest in patients with a severe and critical illness. In multiple regression analyses, only IgA levels were statistically significantly correlated with critical disease (p = .05) regardless of age, sex, and duration of symptoms. Among 100 ambulatory hospital employees who had antibody testing after 4 weeks PoS only 10% had positive IgA antibodies. The most frequently isolated isotype in sera of employees after 30 days PoS was IgG (88%). IgA was the predominant immunoglobulin in early disease and correlated independently with a critical illness. IgG antibodies remained detectable in almost 90% of samples collected up to two months after infection.
MeSH term(s)	Adult ; Aged ; Aged, 80 and over ; Antibodies, Viral/blood ; COVID-19/blood ; COVID-19/diagnosis ; COVID-19/immunology ; COVID-19/mortality ; COVID-19 Serological Testing ; Convalescence ; Enzyme-Linked Immunosorbent Assay ; Female ; Humans ; Immunoglobulin A/blood ; Immunoglobulin G/blood ; Immunoglobulin M/blood ; Male ; Middle Aged ; SARS-CoV-2/immunology ; Sensitivity and Specificity ; Severity of Illness Index ; Survival Analysis
Chemical Substances	Antibodies, Viral ; Immunoglobulin A ; Immunoglobulin G ; Immunoglobulin M
Language	English
Publishing date	2021-05-12
Publishing country	United States
Document type	Journal Article
ZDB-ID	752392-0
ISSN	1096-9071 ; 0146-6615
ISSN (online)	1096-9071
ISSN	0146-6615
DOI	10.1002/jmv.27058
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Zs.A 1320: Show issues

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Article ; Online: Limitations and Confusing Aspects of Diagnostic Testing for Neurologic Lyme Disease in the United States.

Theel, Elitza S / Aguero-Rosenfeld, Maria E / Pritt, Bobbi / Adem, Patricia V / Wormser, Gary P

Journal of clinical microbiology

2019 Volume 57, Issue 1

Abstract: In the United States, laboratories frequently offer multiple different assays for testing of cerebrospinal fluid (CSF) samples to provide laboratory support for the diagnosis of central nervous system Lyme disease (CNSLD). Often included among these ... ...

Abstract	In the United States, laboratories frequently offer multiple different assays for testing of cerebrospinal fluid (CSF) samples to provide laboratory support for the diagnosis of central nervous system Lyme disease (CNSLD). Often included among these diagnostic tests are the same enzyme immunoassays and immunoblots that are routinely used to detect the presence of antibodies to
MeSH term(s)	Antibodies, Bacterial/blood ; Antibodies, Bacterial/cerebrospinal fluid ; Borrelia burgdorferi/immunology ; Diagnosis, Differential ; Diagnostic Tests, Routine/standards ; Humans ; Immunoenzyme Techniques ; Immunoglobulin G/blood ; Immunoglobulin G/cerebrospinal fluid ; Lyme Disease/blood ; Lyme Disease/cerebrospinal fluid ; Lyme Disease/diagnosis ; Lyme Neuroborreliosis/blood ; Lyme Neuroborreliosis/cerebrospinal fluid ; Lyme Neuroborreliosis/diagnosis ; United States
Chemical Substances	Antibodies, Bacterial ; Immunoglobulin G
Language	English
Publishing date	2019-01-02
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	390499-4
ISSN	1098-660X ; 0095-1137
ISSN (online)	1098-660X
ISSN	0095-1137
DOI	10.1128/JCM.01406-18
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Zs.A 1188: Show issues

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Article: Lyme disease: laboratory issues.

Aguero-Rosenfeld, Maria E

Infectious disease clinics of North America

2008 Volume 22, Issue 2, Page(s) 301–13, vii

Abstract: This article describes the laboratory modalities available to confirm the diagnosis of Lyme borreliosis. Use and limitations of these methods are discussed. Current guidelines for the use of recommended serologic methods and discussion of newer methods ... ...

Abstract	This article describes the laboratory modalities available to confirm the diagnosis of Lyme borreliosis. Use and limitations of these methods are discussed. Current guidelines for the use of recommended serologic methods and discussion of newer methods also are provided.
MeSH term(s)	Animals ; Antibodies, Bacterial/analysis ; Antigens, Bacterial/analysis ; Borrelia burgdorferi/genetics ; Borrelia burgdorferi/immunology ; Borrelia burgdorferi/isolation & purification ; DNA, Bacterial/analysis ; DNA, Bacterial/blood ; DNA, Bacterial/cerebrospinal fluid ; Enzyme-Linked Immunosorbent Assay ; Humans ; Immunodominant Epitopes/analysis ; Lyme Disease/diagnosis ; Polymerase Chain Reaction ; Skin/microbiology ; Synovial Fluid/microbiology
Chemical Substances	Antibodies, Bacterial ; Antigens, Bacterial ; DNA, Bacterial ; Immunodominant Epitopes
Language	English
Publishing date	2008-06
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	1077676-x
ISSN	1557-9824 ; 0891-5520
ISSN (online)	1557-9824
ISSN	0891-5520
DOI	10.1016/j.idc.2007.12.005
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Se 1187: Show issues

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Article ; Online: Lyme disease: diagnostic issues and controversies.

Aguero-Rosenfeld, Maria E / Wormser, Gary P

Expert review of molecular diagnostics

2015 Volume 15, Issue 1, Page(s) 1–4

Abstract: The diagnosis of Lyme disease is a controversial topic. Most practitioners and scientists recognize that Lyme disease is associated with certain objective clinical manifestations supported by laboratory evidence of infection with Borrelia burgdorferi ... ...

Abstract	The diagnosis of Lyme disease is a controversial topic. Most practitioners and scientists recognize that Lyme disease is associated with certain objective clinical manifestations supported by laboratory evidence of infection with Borrelia burgdorferi sensu lato (the etiologic agent). There are others, however, who believe that patients with Lyme disease may have a wide variety of entirely nonspecific symptoms without any objective clinical manifestation and that laboratory evidence of infection by B. burgdorferi is not required to support the diagnosis. In reality, this perspective is not evidence based and would inevitably lead to innumerable misdiagnoses, given the high frequency of medically unexplained symptoms, such as fatigue and musculoskeletal pains, in the general population. Although those espousing this viewpoint do not believe that a positive laboratory test is required, nevertheless, they often seek out and promote alternative, unapproved testing methods that frequently provide false-positive results to justify their diagnosis. Herein, we provide a brief overview of Lyme disease testing, emphasizing current usage and limitations. We also discuss the use of nonvalidated procedures and the prospects for a reduction in such testing practices in the future.
MeSH term(s)	Animals ; Antibodies, Bacterial/blood ; Borrelia burgdorferi/immunology ; Diagnostic Errors ; Humans ; Lyme Disease/diagnosis ; Lyme Disease/immunology ; Lyme Disease/microbiology
Chemical Substances	Antibodies, Bacterial
Language	English
Publishing date	2015-01
Publishing country	England
Document type	Editorial ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	2112530-2
ISSN	1744-8352 ; 1473-7159
ISSN (online)	1744-8352
ISSN	1473-7159
DOI	10.1586/14737159.2015.989837
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Zs.A 6073: Show issues

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Article ; Online: Post-COVID-19 inflammatory syndrome manifesting as refractory status epilepticus.

Carroll, Elizabeth / Neumann, Henry / Aguero-Rosenfeld, Maria E / Lighter, Jennifer / Czeisler, Barry M / Melmed, Kara / Lewis, Ariane

Epilepsia

2020 Volume 61, Issue 10, Page(s) e135–e139

Abstract: There have been multiple descriptions of seizures during the acute infectious period in patients with COVID-19. However, there have been no reports of status epilepticus after recovery from COVID-19 infection. Herein, we discuss a patient with refractory ...

Abstract	There have been multiple descriptions of seizures during the acute infectious period in patients with COVID-19. However, there have been no reports of status epilepticus after recovery from COVID-19 infection. Herein, we discuss a patient with refractory status epilepticus 6 weeks after initial infection with COVID-19. Extensive workup demonstrated elevated inflammatory markers, recurrence of a positive nasopharyngeal SARS-CoV-2 polymerase chain reaction, and hippocampal atrophy. Postinfectious inflammation may have triggered refractory status epilepticus in a manner similar to the multisystemic inflammatory syndrome observed in children after COVID-19.
MeSH term(s)	Aged ; COVID-19/complications ; Drug Resistant Epilepsy/virology ; Female ; Humans ; Inflammation/virology ; SARS-CoV-2 ; Status Epilepticus/virology ; Syndrome
Keywords	covid19
Language	English
Publishing date	2020-09-18
Publishing country	United States
Document type	Case Reports ; Journal Article
ZDB-ID	216382-2
ISSN	1528-1167 ; 0013-9580
ISSN (online)	1528-1167
ISSN	0013-9580
DOI	10.1111/epi.16683
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Zs.A 517: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
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Article ; Online: Association of SARS-CoV-2 Genomic Load with Outcomes in Patients with COVID-19.

Zacharioudakis, Ioannis M / Prasad, Prithiv J / Zervou, Fainareti N / Basu, Atreyee / Inglima, Kenneth / Weisenberg, Scott A / Aguero-Rosenfeld, Maria E

Annals of the American Thoracic Society

2020 Volume 18, Issue 5, Page(s) 900–903

MeSH term(s)	COVID-19/complications ; COVID-19/diagnosis ; COVID-19/epidemiology ; COVID-19/therapy ; COVID-19 Nucleic Acid Testing/methods ; Female ; Humans ; Male ; Middle Aged ; New York City/epidemiology ; Outcome and Process Assessment, Health Care/methods ; Outcome and Process Assessment, Health Care/statistics & numerical data ; Patient Discharge/statistics & numerical data ; Risk Assessment/methods ; Risk Factors ; SARS-CoV-2/genetics ; SARS-CoV-2/isolation & purification ; Severity of Illness Index ; Survival Analysis ; Viral Load/methods ; Viral Proteins/isolation & purification
Chemical Substances	Viral Proteins
Language	English
Publishing date	2020-11-19
Publishing country	United States
Document type	Letter
ZDB-ID	2717461-X
ISSN	2325-6621 ; 1943-5665 ; 2325-6621
ISSN (online)	2325-6621 ; 1943-5665
ISSN	2325-6621
DOI	10.1513/AnnalsATS.202008-931RL
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Zs.A 5828: Show issues

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Article ; Online: Early Results from Severe Acute Respiratory Syndrome Coronavirus 2 Polymerase Chain Reaction Testing of Healthcare Workers at an Academic Medical Center in New York City.

Nagler, Arielle R / Goldberg, Eric R / Aguero-Rosenfeld, Maria E / Cangiarella, Joan / Kalkut, Gary / Monahan, Carolyn Rooke / Cerfolio, Robert J

Clinical infectious diseases : an official publication of the Infectious Diseases Society of America

2020 Volume 72, Issue 7, Page(s) 1241–1243

Abstract: Coronavirus disease 2019 (COVID-19) reverse-transcription polymerase chain reaction employee testing was implemented across New York University Langone Health. Over 8 weeks, 14 764 employees were tested; 33% of symptomatic employees, 8% of asymptomatic ... ...

Abstract	Coronavirus disease 2019 (COVID-19) reverse-transcription polymerase chain reaction employee testing was implemented across New York University Langone Health. Over 8 weeks, 14 764 employees were tested; 33% of symptomatic employees, 8% of asymptomatic employees reporting COVID-19 exposure, and 3% of employees returning to work were positive. Positivity rates declined over time, possibly reflecting the importance of community transmission and efficacy of personal protective equipment.
MeSH term(s)	Academic Medical Centers ; COVID-19 ; Health Personnel ; Humans ; New York City/epidemiology ; Polymerase Chain Reaction ; SARS-CoV-2
Keywords	covid19
Language	English
Publishing date	2020-06-25
Publishing country	United States
Document type	Journal Article
ZDB-ID	1099781-7
ISSN	1537-6591 ; 1058-4838
ISSN (online)	1537-6591
ISSN	1058-4838
DOI	10.1093/cid/ciaa867
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Zs.A 1505: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
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