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Article: m6A modification promotes miR-133a repression during cardiac development and hypertrophy via IGF2BP2.

Qian, Benheng / Wang, Ping / Zhang, Donghong / Wu, Lianpin

Cell death discovery

2021 Volume 7, Issue 1, Page(s) 157

Abstract: Both N6-methyladenosine (m6A) RNA modification and microRNAs (miRNAs) are common regulatory mechanisms for gene post-transcription by modulating mRNA stability and translation. They also share the same 3'-untranslated regions (UTRs) regions for their ... ...

Abstract	Both N6-methyladenosine (m6A) RNA modification and microRNAs (miRNAs) are common regulatory mechanisms for gene post-transcription by modulating mRNA stability and translation. They also share the same 3'-untranslated regions (UTRs) regions for their target gene. However, little is known about their potential interaction in cell development and biology. Here, we aimed to investigate how m6A regulates the specific miRNA repression during cardiac development and hypertrophy. Our multiple lines of bioinformatic and molecular biological evidence have shown that m6A modification on cardiac miR-133a target sequence promotes miR-133a repressive effect via AGO2-IGF2BP2 (Argonaute 2-Insulin-like growth factor 2 mRNA binding protein 2) complex. Among 139 cardiac miRNAs, only the seed sequence of miR-133a was inversely complement to m6A consensus motif "GGACH" by sequence alignment analysis. Immunofluorescence staining, luciferase reporter, and m6A-RIP (RNA immunoprecipitation) assays revealed that m6A modification facilitated miR-133a binding to and repressing their targets. The inhibition of the miR-133a on cardiac proliferation and hypertrophy could be prevented by silencing of Fto (FTO alpha-ketoglutarate dependent dioxygenase) which induced m6A modification. IGF2BP2, an m6A binding protein, physically interacted with AGO2 and increased more miR-133a accumulation on its target site, which was modified by m6A. In conclusion, our study revealed a novel and precise regulatory mechanism that the m6A modification promoted the repression of specific miRNA during heart development and hypertrophy. Targeting m6A modification might provide a strategy to repair hypertrophic gene expression induced by miR-133a.
Language	English
Publishing date	2021-06-26
Publishing country	United States
Document type	Journal Article
ISSN	2058-7716
ISSN	2058-7716
DOI	10.1038/s41420-021-00552-7
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Mechanism of balloon burst during transcatheter aortic valve replacement pre-dilatation: Image observation and validation by finite element analysis.

Zhang, Xinmin / Wu, Haozhe / Pan, Zhifang / Elkoumy, Ahmed / Ruan, Zhisheng / Wu, Tianbo / Wu, Daozhu / Soliman, Osama / Wu, Lianpin / Wu, Xinlei

Computers in biology and medicine

2023 Volume 168, Page(s) 107714

Abstract: Background: Balloon burst during transcatheter aortic valve replacement (TAVR) is serious complication. This study pioneers a novel approach by combining image observation and computer simulation validation to unravel the mechanism of balloon burst in a ...

Abstract	Background: Balloon burst during transcatheter aortic valve replacement (TAVR) is serious complication. This study pioneers a novel approach by combining image observation and computer simulation validation to unravel the mechanism of balloon burst in a patient with bicuspid aortic valve (BAV) stenosis. Method: A new computational model for balloon pre-dilatation was developed by incorporating the element failure criteria according to the Law of Laplace. The effects of calcification and aortic tissue material parameters, friction coefficients, balloon types and aortic anatomy classification were performed to validate and compare the expansion behavior and rupture mode of actual balloon. Results: Balloon burst was dissected into three distinct stages based on observable morphological changes. The mechanism leading to the complete transverse burst of the non-compliant balloon initiated at the folding edges, where contacted with heavily calcified masses at the right coronary sinus, resulting in high maximum principal stress. Local sharp spiked calcifications facilitated rapid crack propagation. The elastic moduli of calcification significantly influenced balloon expansion behavior and crack morphology. The simulation case of the calcific elastic modulus was set at 12.6 MPa could closely mirror clinical appearance of expansion behavior and crack pattern. Furthermore, the case of semi-compliant balloons introduced an alternative rupture mechanism as pinhole rupture, driven by local sharp spiked calcifications. Conclusions: The computational model of virtual balloons could effectively simulate balloon dilation behavior and burst mode during TAVR pre-dilation. Further research with a larger cohort is needed to investigate the balloon morphology during pre-dilation by using this method to guide prosthesis sizing for potential favorable outcomes.
MeSH term(s)	Humans ; Transcatheter Aortic Valve Replacement/methods ; Aortic Valve/diagnostic imaging ; Aortic Valve/surgery ; Dilatation ; Computer Simulation ; Finite Element Analysis ; Aortic Valve Stenosis/diagnostic imaging ; Aortic Valve Stenosis/surgery ; Heart Valve Diseases ; Calcinosis/diagnostic imaging ; Calcinosis/surgery ; Treatment Outcome ; Heart Valve Prosthesis ; Prosthesis Design
Language	English
Publishing date	2023-11-23
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	127557-4
ISSN	1879-0534 ; 0010-4825
ISSN (online)	1879-0534
ISSN	0010-4825
DOI	10.1016/j.compbiomed.2023.107714
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Article ; Online: Plasma Cell-Free DNA as a Novel Biomarker for the Diagnosis and Monitoring of Atherosclerosis.

Qian, Benheng / Li, Kexin / Lou, Xiaoying / Guo, Ye / Wang, Yidong / Wu, Lianpin / Zhang, Donghong

Cells

2022 Volume 11, Issue 20

Abstract: Atherosclerosis (AS) is the leading cause of cardiovascular diseases (CVDs) with a high rate of mortality worldwide. Plasma cell-free DNA (cfDNA), mainly originating from apoptosis, necrosis, and active secretion, has been recognized as a promising ... ...

Abstract	Atherosclerosis (AS) is the leading cause of cardiovascular diseases (CVDs) with a high rate of mortality worldwide. Plasma cell-free DNA (cfDNA), mainly originating from apoptosis, necrosis, and active secretion, has been recognized as a promising biomarker for the diagnosis and prognosis of multiple cancers, whereas there are no reports about cfDNA in CVDs. Here, we found an increased quantity and decreased integrity of cfDNA (cfDI) in the serum from AS patients compared with normal controls. Moreover, the reduced cfDI is inversely correlated with serum LDL levels, carotid plaque size, and carotid plaque thickness in the progression of AS. Consistently, in vivo experiments confirmed that the release and cleavage of cfDNA were increased concomitantly with the development and progression of AS in ApoE
MeSH term(s)	Animals ; Mice ; Apolipoproteins E/genetics ; Atherosclerosis/blood ; Atherosclerosis/diagnosis ; Biomarkers, Tumor/genetics ; Cell-Free Nucleic Acids/blood ; Neoplasms/diagnosis
Chemical Substances	Apolipoproteins E ; Biomarkers, Tumor ; Cell-Free Nucleic Acids
Language	English
Publishing date	2022-10-16
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2661518-6
ISSN	2073-4409 ; 2073-4409
ISSN (online)	2073-4409
ISSN	2073-4409
DOI	10.3390/cells11203248
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Article ; Online: Ferroptosis Associates With Diagnosis and Prognosis by Promoting Antitumor Immune Response in Melanoma

Benheng Qian / Kui Wu / Xiaoying Lou / kexin Li / Lianpin Wu / Donghong Zhang

Frontiers in Cell and Developmental Biology, Vol

2022 Volume 10

Abstract: Immunotherapy has greatly improved the clinical benefits of cancer treatment, especially in melanoma. Ferroptosis is a novel mechanism of cell death which relates to immunity. This study aimed at understanding the potential link between ferroptosis and ... ...

Abstract	Immunotherapy has greatly improved the clinical benefits of cancer treatment, especially in melanoma. Ferroptosis is a novel mechanism of cell death which relates to immunity. This study aimed at understanding the potential link between ferroptosis and cancer immunocompetent in melanoma using multiple bioinformatics analyses. By the WGCNA assay, we first constructed a key module–gene of ferroptosis, which was strongly correlated with the diagnosis, prognosis, and infiltration of immune cells in melanoma. The elevated module–gene could effectively distinguish melanoma from normal tissues and acted as a good prognostic marker. The module–gene of ferroptosis was positively correlated with the infiltration of immune cells. In particular, the module was positively correlated with the expression of PD-L1 and sensitively increased after effective anti-PD-1 treatment. Furthermore, the differential expression of the module–gene between normal and tumor tissues was observed in pan-cancer. The similarity correlations of the module–gene with infiltration of immune cells and the expressions of PD-L1 were confirmed in the pan-cancer level. Our study demonstrated that the key module–gene of ferroptosis was closely related with diagnosis, prognosis, and anti-immune response in melanoma, as well as in pan-cancer.
Keywords	ferroptosis ; melanoma ; cancer immunity ; immunotherapy ; pan-cancer ; Biology (General) ; QH301-705.5
Subject code	570
Language	English
Publishing date	2022-07-01T00:00:00Z
Publisher	Frontiers Media S.A.
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Ferroptosis Associates With Diagnosis and Prognosis by Promoting Antitumor Immune Response in Melanoma.

Qian, Benheng / Wu, Kui / Lou, Xiaoying / Li, Kexin / Wu, Lianpin / Zhang, Donghong

Frontiers in cell and developmental biology

2022 Volume 10, Page(s) 915198

Abstract	Immunotherapy has greatly improved the clinical benefits of cancer treatment, especially in melanoma. Ferroptosis is a novel mechanism of cell death which relates to immunity. This study aimed at understanding the potential link between ferroptosis and cancer immunocompetent in melanoma using multiple bioinformatics analyses. By the WGCNA assay, we first constructed a key module-gene of ferroptosis, which was strongly correlated with the diagnosis, prognosis, and infiltration of immune cells in melanoma. The elevated module-gene could effectively distinguish melanoma from normal tissues and acted as a good prognostic marker. The module-gene of ferroptosis was positively correlated with the infiltration of immune cells. In particular, the module was positively correlated with the expression of PD-L1 and sensitively increased after effective anti-PD-1 treatment. Furthermore, the differential expression of the module-gene between normal and tumor tissues was observed in pan-cancer. The similarity correlations of the module-gene with infiltration of immune cells and the expressions of PD-L1 were confirmed in the pan-cancer level. Our study demonstrated that the key module-gene of ferroptosis was closely related with diagnosis, prognosis, and anti-immune response in melanoma, as well as in pan-cancer.
Language	English
Publishing date	2022-07-08
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2737824-X
ISSN	2296-634X
ISSN	2296-634X
DOI	10.3389/fcell.2022.915198
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Article ; Online: Correction：Diagnostic Accuracy of Chest Computed Tomography Scans for Suspected Patients With COVID-19: Receiver Operating Characteristic Curve Analysis.

Wu, Lianpin / Jin, Qike / Chen, Jie / He, Jiawei / Brett-Major, David M / Dong, Jianghu James

JMIR public health and surveillance

2020 Volume 6, Issue 4, Page(s) e25829

Abstract: This corrects the article DOI: 10.2196/19424.]. ...

Abstract	[This corrects the article DOI: 10.2196/19424.].
Language	English
Publishing date	2020-11-20
Publishing country	Canada
Document type	Published Erratum
ISSN	2369-2960
ISSN (online)	2369-2960
DOI	10.2196/25829
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Article ; Online: Analysis of the incidence and influencing factors associated with binary restenosis of target lesions after drug-coated balloon angioplasty for patients with in-stent restenosis.

Xue, Weihao / Ma, Jun / Yu, Xiaojie / Ruan, Zhisheng / Sun, Yuanxue / Wu, Tianbo / Zhang, Xinmin / Wu, Lianpin

BMC cardiovascular disorders

2022 Volume 22, Issue 1, Page(s) 493

Abstract: Background: Drug-coated balloon (DCB) is a novel and effective device for coronary artery disease patients with in-stent restenosis (ISR). However, the incidence and possible influencing factors associated with binary restenosis have not yet been ... ...

Abstract	Background: Drug-coated balloon (DCB) is a novel and effective device for coronary artery disease patients with in-stent restenosis (ISR). However, the incidence and possible influencing factors associated with binary restenosis have not yet been adequately assessed. Methods: The data are extracted from a prospective, multicenter, randomized controlled trial. A total of 211 patients with ISR were enrolled at 13 centers from August 2017 to October 2018 and treated with DCB. At the 9-month coronary angiographic follow-up, patients were divided into restenosis and non-restenosis groups, and demographic data, lesion features, and laboratory tests were retrospectively reviewed. Furthermore, logistic regression analysis was used to identify possible influencing factors. Results: All patients successfully underwent treatment, and 166 patients with 190 lesions took part in angiography follow-ups at 9 months. Of these, 41 patients with 44 target lesions developed restenosis following treatment, and the incidence of ISR was 24.7%. There were significant differences in the average length of target lesions and the number of multivessel lesions and fasting plasma glucose (FBG) between the two groups (p < 0.05). Demographic data, cardiac risk factors, left ventricular ejection fractions (LVEF), blood routine tests, biochemical tests, and other features of devices and lesions showed no difference. Logistic regression analyses showed that FBG > 6.1 mmol/L (OR: 7.185 95% CI: 2.939-17.567 P < 0.001) and length of lesion (OR:1.046 95% CI: 1.001-1.093 P = 0.046) were associated risk factors. Conclusions: The longer length of lesions, more target lesions and FBG > 6.1 mmol/L per individual may be characteristics of patients showing ISR following treatment. Studies with larger sample size, and more complete follow-up data are needed in the future to expend on these findings. Trial registration: No.: NCT04213378, first posted date (30/12/2019).
MeSH term(s)	Humans ; Coronary Restenosis/diagnostic imaging ; Coronary Restenosis/epidemiology ; Coronary Restenosis/etiology ; Incidence ; Retrospective Studies ; Prospective Studies ; Angioplasty, Balloon/adverse effects ; Constriction, Pathologic/complications
Language	English
Publishing date	2022-11-20
Publishing country	England
Document type	Randomized Controlled Trial ; Multicenter Study ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2059859-2
ISSN	1471-2261 ; 1471-2261
ISSN (online)	1471-2261
ISSN	1471-2261
DOI	10.1186/s12872-022-02923-z
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Changes of N6-methyladenosine modulators promote breast cancer progression.

Wu, Lianpin / Wu, Dengying / Ning, Jinfeng / Liu, Wei / Zhang, Donghong

BMC cancer

2019 Volume 19, Issue 1, Page(s) 326

Abstract: Background: Breast cancer (BC) displays striking genetic, epigenetic and phenotypic diversity. N: Methods: The expression of m6A methylases (METTL3, METTL14 and WTAP) and demethylases (FTO and ALKBH5) were analyzed by using ONCOMINE and The Cancer ... ...

Abstract	Background: Breast cancer (BC) displays striking genetic, epigenetic and phenotypic diversity. N Methods: The expression of m6A methylases (METTL3, METTL14 and WTAP) and demethylases (FTO and ALKBH5) were analyzed by using ONCOMINE and The Cancer Genome Atlas databases and in 36 pairs of BC and adjacent non-cancerous tissue. The level of m6A in BC patients was detected by ELISA, and the function of m6A was analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, colony formation assay and transwell assay. The database of bc-GenExMiner v4.0, Kaplan-Meier Plotter and cBioPortal were queried for correlation, mutation and prognosis analysis of BC. Results: The m6A methylases and demethylases were dysregulated in several major malignant tumors. Specifically, the expression of all m6A methylases was reduced in BC as compared with normal controls, but the demethylase ALKBH5 was induced in ONCOMINE databases and confirmed in clinical patients. METTL14 expression was positively correlated with METTL3 expression, and both showed high expression in normal breast-like and luminal-A and -B BC. Functionally, reducing m6A expression by overexpressing METTL14 and/or knockdown of ALKBH5 could inhibit breast cell viability, colony formation and cell migration. Furthermore, Kaplan-Meier, meta-analysis and univariate Cox assay showed that the expression of m6A members including METTL3, METTL14, WTAP and FTO but not their gene mutation and amplification, was tightly associated with cancer progression and poor survival. Conclusions: Changes of m6A modulators reduced m6A may promote tumorigenesis and predict poor prognosis in BC.
MeSH term(s)	Adenosine/analogs & derivatives ; Adenosine/metabolism ; Adult ; Breast/pathology ; Breast Neoplasms/diagnosis ; Breast Neoplasms/genetics ; Breast Neoplasms/mortality ; Breast Neoplasms/pathology ; Carcinogenesis/genetics ; Cell Line, Tumor ; Datasets as Topic ; Disease Progression ; Epigenesis, Genetic ; Female ; Gene Expression Profiling ; Gene Expression Regulation, Neoplastic ; Humans ; Kaplan-Meier Estimate ; Methylation ; Methyltransferases/genetics ; Methyltransferases/metabolism ; Middle Aged ; Mutation ; Oxidoreductases, N-Demethylating/genetics ; Oxidoreductases, N-Demethylating/metabolism ; Prognosis ; RNA, Messenger/metabolism
Chemical Substances	RNA, Messenger ; N-methyladenosine (CLE6G00625) ; Oxidoreductases, N-Demethylating (EC 1.5.-) ; Methyltransferases (EC 2.1.1.-) ; Adenosine (K72T3FS567)
Language	English
Publishing date	2019-04-05
Publishing country	England
Document type	Journal Article
ISSN	1471-2407
ISSN (online)	1471-2407
DOI	10.1186/s12885-019-5538-z
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Article ; Online: Diagnostic Accuracy of Chest Computed Tomography Scans for Suspected Patients With COVID-19: Receiver Operating Characteristic Curve Analysis.

Wu, Lianpin / Jin, Qike / Chen, Jie / He, Jiawei / Brett-Major, David M / Dong, Jianghu James

JMIR public health and surveillance

2020 Volume 6, Issue 4, Page(s) e19424

Abstract: Background: Computed tomography (CT) scans are increasingly available in clinical care globally. They enable a rapid and detailed assessment of tissue and organ involvement in disease processes that are relevant to diagnosis and management, particularly ...

Abstract	Background: Computed tomography (CT) scans are increasingly available in clinical care globally. They enable a rapid and detailed assessment of tissue and organ involvement in disease processes that are relevant to diagnosis and management, particularly in the context of the COVID-19 pandemic. Objective: The aim of this paper is to identify differences in the CT scan findings of patients who were COVID-19 positive (confirmed via nucleic acid testing) to patients who were confirmed COVID-19 negative. Methods: A retrospective cohort study was proposed to compare patient clinical characteristics and CT scan findings in suspected COVID-19 cases. A multivariable logistic model with LASSO (least absolute shrinkage and selection operator) selection for variables was used to identify the good predictors from all available predictors. The area under the curve (AUC) with 95% CI was calculated for each of the selected predictors and the combined selected key predictors based on receiver operating characteristic curve analysis. Results: A total of 94 (56%) patients were confirmed positive for COVID-19 from the suspected 167 patients. We found that elderly people were more likely to be infected with COVID-19. Among the 94 confirmed positive patients, 2 (2%) patients were admitted to an intensive care unit. No patients died during the study period. We found that the presence, distribution, and location of CT lesions were associated with the presence of COVID-19. White blood cell count, cough, and a travel history to Wuhan were also the top predictors for COVID-19. The overall AUC of these selected predictors is 0.97 (95% CI 0.93-1.00). Conclusions: Taken together with nucleic acid testing, we found that CT scans can allow for the rapid diagnosis of COVID-19. This study suggests that chest CT scans should be more broadly adopted along with nucleic acid testing in the initial assessment of suspected COVID-19 cases, especially for patients with nonspecific symptoms.
MeSH term(s)	Adolescent ; Adult ; COVID-19 Testing ; Clinical Laboratory Techniques/methods ; Coronavirus Infections/diagnosis ; Female ; Humans ; Male ; Middle Aged ; ROC Curve ; Reproducibility of Results ; Retrospective Studies ; Thorax/diagnostic imaging ; Tomography, X-Ray Computed ; Young Adult
Keywords	covid19
Language	English
Publishing date	2020-10-20
Publishing country	Canada
Document type	Journal Article
ISSN	2369-2960
ISSN (online)	2369-2960
DOI	10.2196/19424
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Article ; Online: Correction：Diagnostic Accuracy of Chest Computed Tomography Scans for Suspected Patients With COVID-19

Wu, Lianpin / Jin, Qike / Chen, Jie / He, Jiawei / Brett-Major, David M / Dong, Jianghu James

JMIR Public Health and Surveillance, Vol 6, Iss 4, p e

Receiver Operating Characteristic Curve Analysis

2020 Volume 25829

Keywords	Public aspects of medicine ; RA1-1270
Language	English
Publishing date	2020-11-01T00:00:00Z
Publisher	JMIR Publications
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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