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Article: B-cell-depleted patients with persistent SARS-CoV-2 infection: combination therapy or monotherapy? A real-world experience.

D'Abramo, Alessandra / Vita, Serena / Beccacece, Alessia / Navarra, Assunta / Pisapia, Raffaella / Fusco, Francesco Maria / Matusali, Giulia / Girardi, Enrico / Maggi, Fabrizio / Goletti, Delia / Nicastri, Emanuele

Frontiers in medicine

2024 Volume 11, Page(s) 1344267

Abstract: Objectives: The aim of the study was to describe a cohort of B-cell-depleted immunocompromised (IC) patients with prolonged or relapsing COVID-19 treated with monotherapy or combination therapy.: Methods: This is a multicenter observational ... ...

Abstract	Objectives: The aim of the study was to describe a cohort of B-cell-depleted immunocompromised (IC) patients with prolonged or relapsing COVID-19 treated with monotherapy or combination therapy. Methods: This is a multicenter observational retrospective study conducted on IC patients consecutively hospitalized with a prolonged or relapsing SARS-CoV-2 infection from November 2020 to January 2023. IC COVID-19 subjects were stratified according to the monotherapy or combination anti-SARS-CoV-2 therapy received. Results: Eighty-eight patients were enrolled, 19 under monotherapy and 69 under combination therapy. The study population had a history of immunosuppression (median of 2 B-cells/mm Conclusion: In IC persistent COVID-19 patients, it is essential to explore new therapeutic strategies such as combination multi-target therapy (antiviral or double antiviral plus antibody-based therapies) to avoid persistent viral shedding and/or severe SARS-CoV-2 infection.
Language	English
Publishing date	2024-02-29
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2775999-4
ISSN	2296-858X
ISSN	2296-858X
DOI	10.3389/fmed.2024.1344267
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Article: Relapsing Neurological Complications in a Child With ATP1A3 Gene Mutation and Influenza Infection: A Case Report.

Pisapia, Raffaella / Capoluongo, Nicolina / Palmiero, Giulia / Tascini, Carlo / Rescigno, Carolina

Frontiers in neurology

2021 Volume 12, Page(s) 774054

Abstract: Mutations in the ATP1A3 gene encoding the α3 subunit of Na+/K+-ATPase are associated with different neurological manifestations that may be elicited by febrile episodes. A recently described phenotype, linked to the p.Arg756Cys mutation, is clinically ... ...

Abstract	Mutations in the ATP1A3 gene encoding the α3 subunit of Na+/K+-ATPase are associated with different neurological manifestations that may be elicited by febrile episodes. A recently described phenotype, linked to the p.Arg756Cys mutation, is clinically characterized by Relapsing Encephalopathy with Cerebellar Ataxia (RECA). In our case, a diagnosis of RECA has been established, and despite an alternative, reasonable cause had been already identified. We describe the case of a child with two recurrent episodes, 2 years apart, of hypotonia and ataxia. In both episodes, a laboratory-confirmed influenza virus infection suggested the diagnosis of influenza-associated encephalopathy. After the second episode, a search for genetic mutations was performed, and ATP1A3 mutation associated to RECA was found. After both episodes, the child was discharged after partial improvement of neurological conditions. The diagnosis of encephalopathy in children is often challenging. A genetic predisposition to neurological decompensation should be suspected in case of recurrent episodes, even if an alternative diagnosis has been established. Indeed, febrile infections may only represent the trigger of neurological involvement. In these patients, the knowledge of a genetic predisposing factors may help in the prevention of neurological episodes by the prompt use of anti-pyrectics and preventive measures as appropriate vaccination.
Language	English
Publishing date	2021-12-15
Publishing country	Switzerland
Document type	Case Reports
ZDB-ID	2564214-5
ISSN	1664-2295
ISSN	1664-2295
DOI	10.3389/fneur.2021.774054
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Article ; Online: Human papillomavirus genotyping in high-grade vaginal intraepithelial neoplasia: A multicentric Italian study.

Preti, Mario / Boldorini, Renzo / Gallio, Niccolò / Cavagnetto, Cristina / Borella, Fulvio / Pisapia, Elena / Ribaldone, Raffaella / Bovio, Enrica / Bertero, Luca / Airoldi, Chiara / Cassoni, Paola / Remorgida, Valentino / Benedetto, Chiara

Journal of medical virology

2024 Volume 96, Issue 2, Page(s) e29474

Abstract: This study aimed to analyze the human papillomavirus (HPV) genotype distribution in a large cohort of high-grade vaginal intraepithelial neoplasia (VaIN) (vaginal HSIL, VaIN2/3) patients from two Italian referral centers. We included all patients with ... ...

Abstract	This study aimed to analyze the human papillomavirus (HPV) genotype distribution in a large cohort of high-grade vaginal intraepithelial neoplasia (VaIN) (vaginal HSIL, VaIN2/3) patients from two Italian referral centers. We included all patients with histologically confirmed VaIN2/3 from the Department of Surgical Sciences, Sant'Anna Hospital, University of Torino, Torino, Italy, and Ospedale Maggiore della Carità, Novara, Italy, between 2003 and 2022. After the histological evaluation of formalin-fixed paraffin-embedded samples, we performed HPV genotyping with VisionArray HPV Chip 1.0. We detected HPV DNA in 94.4% of VaIN2/3 (168/178), with HPV 16 as the most prevalent genotype, accounting for 51.8% of all infections, 41.2% of VaIN2 and 77.6% of VaIN3 cases. Other frequent genotypes were HPV 58 (8.3%, 10.9% of VaIN2 and 2.0% of VaIN3), HPV 73 (5.4%, 5.0% of VaIN2 and 6.1% of VaIN3), and HPV 31 (5.4%, 6.7% of VaIN2 and 2.0% of VaIN3). 73.2% of VaIN2/3 had a single HPV genotype infection and 26.8% a multiple infection (20.8% a double infection, 4.8% a triple infection, and 1.2% a quadruple infection). Single infection was more frequently present in VaIN3 than VaIN2 (81.6% vs. 69.8%). 69.1% of single infections and 73.3% of multiple infections had one or more genotypes covered by nine-valent HPV vaccine. HPV vaccination is expected to have a large impact on reducing the incidence of vaginal intraepithelial neoplasia.
MeSH term(s)	Female ; Humans ; Papillomavirus Infections/epidemiology ; Genotype ; Retrospective Studies ; Vaginal Neoplasms ; Carcinoma in Situ/epidemiology ; Papillomaviridae/genetics ; Human papillomavirus 16 ; Uterine Cervical Neoplasms ; Uterine Cervical Dysplasia
Language	English
Publishing date	2024-02-19
Publishing country	United States
Document type	Journal Article
ZDB-ID	752392-0
ISSN	1096-9071 ; 0146-6615
ISSN (online)	1096-9071
ISSN	0146-6615
DOI	10.1002/jmv.29474
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Article ; Online: Viral Hepatitis: Etiology, Epidemiology, Transmission, Diagnostics, Treatment, and Prevention.

Lanini, Simone / Ustianowski, Andrew / Pisapia, Raffaella / Zumla, Alimuddin / Ippolito, Giuseppe

Infectious disease clinics of North America

2019 Volume 33, Issue 4, Page(s) 1045–1062

Abstract: Viral hepatitis is a major global public health problem affecting hundreds of millions of people and is associated with significant morbidity and mortality. Five major biologically unrelated hepatotropic viruses cause most of the global burden of viral ... ...

Abstract	Viral hepatitis is a major global public health problem affecting hundreds of millions of people and is associated with significant morbidity and mortality. Five major biologically unrelated hepatotropic viruses cause most of the global burden of viral hepatitis. Hepatitis B and hepatitis C are associated with a significant number of chronic infections. Most deaths from viral hepatitis are due to hepatitis B and hepatitis C. An estimated 257 million people were living with HBV and 71 million people were living with HCV. Most people are asymptomatic. New diagnostics and highly effective, pangenotypic direct-acting antivirals provide opportunities to cure and eradicate chronic hepatitis C virus infection.
MeSH term(s)	Global Health ; Hepatitis Viruses/classification ; Hepatitis Viruses/pathogenicity ; Hepatitis, Viral, Human/epidemiology ; Hepatitis, Viral, Human/prevention & control ; Hepatitis, Viral, Human/therapy ; Hepatitis, Viral, Human/virology ; Humans
Language	English
Publishing date	2019-10-31
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	1077676-x
ISSN	1557-9824 ; 0891-5520
ISSN (online)	1557-9824
ISSN	0891-5520
DOI	10.1016/j.idc.2019.08.004
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Article ; Online: Unexpected effects of COVID-19 outbreak: adaption of Anti-Retroviral Therapy (ART) delivery policies improved adherence in a population of People Living With HIV (PLWH).

Fusco, Francesco Maria / Sangiovanni, Nadia / Papa, Nunzia / Mattera Iacono, Valentina / Cuomo, Nunzia / Viglietti, Rosaria / Tambaro, Orsola / Borrelli, Francesco / Pisapia, Raffaella / Carleo, Maria Aurora / Rizzo, Viviana / Spatarella, Micaela / Esposito, Vincenzo / Sangiovanni, Vincenzo

Le infezioni in medicina

2023 Volume 31, Issue 2, Page(s) 204–208

Abstract: Background: Adherence to Anti-Retroviral Therapy (ART) is crucial for People Living With HIV (PLWH). In Italy, ART is delivered by Hospital Pharmacies, on a renewable prescription from the hospital physician. The measurement of package-refill (the rate ... ...

Abstract	Background: Adherence to Anti-Retroviral Therapy (ART) is crucial for People Living With HIV (PLWH). In Italy, ART is delivered by Hospital Pharmacies, on a renewable prescription from the hospital physician. The measurement of package-refill (the rate of ART packages actually collected out of those to be collected in order to comply with therapy) is an effective tool to evaluate the adherence.During COVID-19 outbreak, at "D. Cotugno" hospital in Naples, Italy, the ART delivery policies have been adapted, in order to reduce the number of patients' access. We analysed the impact of these changes on the pill-refill of ART in January-August 2020, compared with 2018-2019. Methods: "D. Cotugno" hospital is a mono-specialistic Infectious Diseases hospital, caring for about 2500 PLWH. Since February 2020, the hospital was almost entirely dedicated to COVID-19 patients. All out-patient activities were interrupted, except for those dedicated to HIV/AIDS patients.In this preliminary study we included all patients assigned to one of the three Medical Divisions dedicated to HIV, who were already under treatment since at least 2017. Rate of package-refill was obtained by the Hospital Pharmacy registry, demographic and clinical data were derived from clinical database.During COVID-19, many measures were adopted in order to increase safety of PLWH attending to hospital. Among these, medical prescription validity increased from 4 to 6 months, and number of packages to be collected increased from 2 to 4, adopting a multi-month dispensing strategy.Package-refill is adequate if at least 95% of ART have been actually collected; partial and inadequate if 75%-94% or less than 75% of ART, respectively, have been collected. Package-refill was measured during the first year of COVID-19 (March 2020 - February 2021), compared to the same period in the two years before. Results: A total of 594 PLWH were included. PLWH with optimal pill-refill significantly increased in 2020-21 compared to 2018-2020 (62% vs 55%, p 0.013). Discussion: Due to COVID-19, we would have expected a reduction in ART deliveries. Surprisingly, the opposite occurred. The increase of pill-refill rates may be due to different reasons, but we hypothesized that the adaption of delivery policies, with a higher number of packages allowed to be collected, strongly contributed to this result. This study suggests that multi-month dispensing policies may contribute to the improvement of adherence among PLWH.
Language	English
Publishing date	2023-06-01
Publishing country	Italy
Document type	Journal Article
ZDB-ID	2041081-5
ISSN	2532-8689 ; 1124-9390
ISSN (online)	2532-8689
ISSN	1124-9390
DOI	10.53854/liim-3102-8
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Article: Predictors of Mortality and Orotracheal Intubation in Patients with Pulmonary Barotrauma Due to COVID-19: An Italian Multicenter Observational Study during Two Years of the Pandemic.

Tetaj, Nardi / De Pascale, Gennaro / Antonelli, Massimo / Vargas, Joel / Savino, Martina / Pugliese, Francesco / Alessandri, Francesco / Giordano, Giovanni / Tozzi, Pierfrancesco / Rocco, Monica / Biava, Anna Maria / Maggi, Luigi / Pisapia, Raffaella / Fusco, Francesco Maria / Stazi, Giulia Valeria / Garotto, Gabriele / Marini, Maria Cristina / Piselli, Pierluca / Beccacece, Alessia /

Mariano, Andrea / Giancola, Maria Letizia / Ianniello, Stefania / Vaia, Francesco / Girardi, Enrico / Antinori, Andrea / Bocci, Maria Grazia / Marchioni, Luisa / Nicastri, Emanuele

Journal of clinical medicine

2024 Volume 13, Issue 6

Abstract: Introduction: ...

Abstract	Introduction:
Language	English
Publishing date	2024-03-15
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662592-1
ISSN	2077-0383
ISSN	2077-0383
DOI	10.3390/jcm13061707
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Article ; Online: Global epidemiology of viral hepatitis and national needs for complete control.

Lanini, Simone / Pisapia, Raffaella / Capobianchi, Maria Rosaria / Ippolito, Giuseppe

Expert review of anti-infective therapy

2018 Volume 16, Issue 8, Page(s) 625–639

Abstract: Introduction: The World Health Organization recognizes that viral hepatitis is not only a massive public health issue but also a huge opportunity to improve quality of life and equity at a global level. Viral hepatitis causes about 1.5 million deaths ... ...

Abstract	Introduction: The World Health Organization recognizes that viral hepatitis is not only a massive public health issue but also a huge opportunity to improve quality of life and equity at a global level. Viral hepatitis causes about 1.5 million deaths each year and significantly affects the quality of life of hundreds of millions of people. To date, frail individuals in high-income countries and people living in low-income settings are paying the heaviest tool. Areas covered. Here we present a broad discussion on current knowledge and topical issues about the hepatitis pandemic. The report includes a structured overview of global epidemiology, including the definition of specific local epidemic profiles for each hepatitis agents (HAV, HBV, HCV, and HEV), and a perspective about the critical actions needed for achieving a complete control. Expert commentary. The control of viral hepatitis is currently, ethically urgent and even economically convenient. There is a wide consensus that viral hepatitis can be controlled through comprehensive intervention tailored on local needs addressing the issue of viral hepatitis as a unique public health issue. These strategies should include: (1) primary prevention (including vaccination and improved infection control), (2) improving diagnosis rate, and (3) management of existing cases of infections.
MeSH term(s)	Antiviral Agents/administration & dosage ; Developed Countries ; Developing Countries ; Global Health ; Hepatitis, Viral, Human/epidemiology ; Hepatitis, Viral, Human/virology ; Humans ; Public Health ; Quality of Life ; World Health Organization
Chemical Substances	Antiviral Agents
Language	English
Publishing date	2018-08-02
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	2181279-2
ISSN	1744-8336 ; 1478-7210
ISSN (online)	1744-8336
ISSN	1478-7210
DOI	10.1080/14787210.2018.1505503
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Article ; Online: Does COVID-19 Vaccination with BNT162b2 Influence HIV-Related Immunological and Virological Markers? Data from 235 Persons Living with HIV at Cotugno Hospital, Naples, Italy: Immune Response After Second and Third Doses, and Influence on Immunovirological Markers.

Fusco, Francesco M / Carleo, Maria Aurora / Sangiovanni, Nadia / D'Abbraccio, Maurizio / Tambaro, Orsola / Borrelli, Francesco / Viglietti, Rosaria / Camaioni, Clarissa / Bruner, Vincenzo / Falanga, Rosa / Pisapia, Raffaella / Palmiero, Giulia / Rizzo, Viviana / Rosario, Pietro / Cuomo, Nunzia / Spatarella, Micaela / Esposito, Vincenzo / Sangiovanni, Vincenzo

Viral immunology

2023 Volume 36, Issue 5, Page(s) 360–365

Abstract: Few data are available on the impact of COVID-19 vaccination on CD4 counts and HIV-RNA in persons living with HIV (PLWH). We present the data of 235 PLWH who were vaccinated with BNT162b2 in March 2021-February 2022 at the "Cotugno" hospital in Naples. ... ...

Abstract	Few data are available on the impact of COVID-19 vaccination on CD4 counts and HIV-RNA in persons living with HIV (PLWH). We present the data of 235 PLWH who were vaccinated with BNT162b2 in March 2021-February 2022 at the "Cotugno" hospital in Naples. PLWH treated at the "Cotugno" hospital, who were vaccinated at the hospital vaccination center, without prior COVID-19 and for whom immunological/virological data were available in the last 12 months and in the 6 months after vaccination were included. Antispike Ab were available for 187 and 64 PLWH after the second and third doses: PLWH with antispikes >33 binding antibodies units (BAU)/mL increased from 91% to 98%. Antinucleocapsid Ab performed in 147 and 56 patients identified 19 (13%) asymptomatic/paucisymptomatic COVID-19 infections after the second dose and an additional 15 (27%) after the third dose. Immunological/virological data were collected before vaccination (T0), after the second dose (T1), and after the third dose (T2). The absolute number of CD4 increased after the third dose (median 663, 657, and 707 at T0, T1, and T2;
MeSH term(s)	Humans ; BNT162 Vaccine ; COVID-19 Vaccines ; RNA, Viral ; COVID-19/prevention & control ; SARS-CoV-2 ; Italy/epidemiology ; Vaccination ; Hospitals ; Immunity ; HIV Infections ; Antibodies, Viral
Chemical Substances	BNT162 Vaccine ; COVID-19 Vaccines ; RNA, Viral ; Antibodies, Viral
Language	English
Publishing date	2023-05-04
Publishing country	United States
Document type	Journal Article
ZDB-ID	639075-4
ISSN	1557-8976 ; 0882-8245
ISSN (online)	1557-8976
ISSN	0882-8245
DOI	10.1089/vim.2022.0182
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Article: Hypernatremia During Intravenous Treatment With Fosfomycin: A Retrospective Medical Record Review Study and an Analysis of Spontaneous Reports in the EudraVigilance Database.

Scavone, Cristina / Mascolo, Annamaria / Bernardi, Francesca Futura / Aiezza, Maria Luisa / Saturnino, Paola / Morra, Gaia / Simonelli, Margherita / Massa, Marida / Pomicino, Andrea / Minei, Giuseppina / Pisapia, Raffaella / Spatarella, Micaela / Trama, Ugo / Guglielmi, Gaspare / Capuano, Annalisa / Perrella, Alessandro

Frontiers in pharmacology

2022 Volume 13, Page(s) 844122

Abstract: Background: ...

Abstract	Background:
Language	English
Publishing date	2022-03-29
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587355-6
ISSN	1663-9812
ISSN	1663-9812
DOI	10.3389/fphar.2022.844122
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Article ; Online: Early predictors of clinical deterioration in a cohort of outpatients with COVID-19 in southern Italy: A multicenter observational study.

Monari, Caterina / Pisaturo, Mariantonietta / Maggi, Paolo / Macera, Margherita / Di Caprio, Giovanni / Pisapia, Raffaella / Gentile, Valeria / Fordellone, Mario / Chiodini, Paolo / Coppola, Nicola

Journal of medical virology

2022 Volume 94, Issue 11, Page(s) 5336–5344

Abstract: Data regarding early predictors of clinical deterioration in patients with infection of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is still scarce. The aim of the study is to identify early symptoms or signs that may be associated with ... ...

Abstract	Data regarding early predictors of clinical deterioration in patients with infection of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is still scarce. The aim of the study is to identify early symptoms or signs that may be associated with severe coronavirus disease 2019 (COVID-19). We conducted a multicentre prospective cohort study on a cohort of patients with COVID-19 in home isolation from March 2020 to April 2021. We assessed longitudinal clinical data (fever, dyspnea, need for hospitalization) through video calls at three specific time points: the beginning of symptoms or the day of the first positivity of the nasopharyngeal swab for SARS-CoV-2-RNA (t
MeSH term(s)	Adult ; Aged ; COVID-19/diagnosis ; COVID-19/epidemiology ; Clinical Deterioration ; Cohort Studies ; Female ; Fever/epidemiology ; Hospitalization ; Humans ; Male ; Middle Aged ; Outpatients ; Prospective Studies ; SARS-CoV-2
Language	English
Publishing date	2022-07-25
Publishing country	United States
Document type	Journal Article ; Multicenter Study ; Observational Study
ZDB-ID	752392-0
ISSN	1096-9071 ; 0146-6615
ISSN (online)	1096-9071
ISSN	0146-6615
DOI	10.1002/jmv.28007
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