LIVIVO - Search results -

Search results

Result 1 - 10 of total 59

Search options

Article ; Online: Sample Processing for Metaproteomic Analysis of Human Gut Microbiota.

García-Durán, Carmen / Martínez-López, Raquel / Monteoliva, Lucía / Gil, Concha

Methods in molecular biology (Clifton, N.J.)

2021 Volume 2420, Page(s) 53–61

Abstract: Human gut microbiota can be studied through the characterization of microorganisms present in feces. Metaproteomics has arisen as a good approach to investigate this vast community. However, the processing of fecal samples in order to obtain the largest ... ...

Abstract	Human gut microbiota can be studied through the characterization of microorganisms present in feces. Metaproteomics has arisen as a good approach to investigate this vast community. However, the processing of fecal samples in order to obtain the largest number of proteins from gut microbiota to be subsequently analyzed by means of metaproteomics is a challenge. Here we describe a protocol to approach this task. It includes two main steps: the first step of humectation and dispersion of the feces, followed by the separation of microorganisms from other fecal components such as roughage and food debris, and the second step in which microbial cells are broken up and microbiota proteins recovered for MS analysis. Detailed procedures for sample preparation, protein extraction, trypsin digestion, and mass spectrometry analysis for gut microbiota samples are provided.
MeSH term(s)	Feces ; Gastrointestinal Microbiome ; Humans ; Proteins ; Proteomics ; Specimen Handling
Chemical Substances	Proteins
Language	English
Publishing date	2021-12-14
Publishing country	United States
Document type	Journal Article
ISSN	1940-6029
ISSN (online)	1940-6029
DOI	10.1007/978-1-0716-1936-0_5
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- Full text online
- Order with fees

Article: Extending the Proteomic Characterization of Candida albicans Exposed to Stress and Apoptotic Inducers through Data-Independent Acquisition Mass Spectrometry.

Amador-García, Ahinara / Zapico, Inés / Borrajo, Ana / Malmström, Johan / Monteoliva, Lucía / Gil, Concha

mSystems

2021 Volume 6, Issue 5, Page(s) e0094621

Abstract: Candida albicans is a commensal fungus that causes systemic infections in immunosuppressed patients. In order to deal with the changing environment during commensalism or infection, C. albicans must reprogram its proteome. Characterizing the stress- ... ...

Abstract	Candida albicans is a commensal fungus that causes systemic infections in immunosuppressed patients. In order to deal with the changing environment during commensalism or infection, C. albicans must reprogram its proteome. Characterizing the stress-induced changes in the proteome that C. albicans uses to survive should be very useful in the development of new antifungal drugs. We studied the C. albicans global proteome after exposure to hydrogen peroxide (H
Language	English
Publishing date	2021-10-05
Publishing country	United States
Document type	Journal Article
ISSN	2379-5077
ISSN	2379-5077
DOI	10.1128/mSystems.00946-21
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Candida albicans Hyphal Extracellular Vesicles Are Different from Yeast Ones, Carrying an Active Proteasome Complex and Showing a Different Role in Host Immune Response.

Martínez-López, Raquel / Hernáez, Maria Luisa / Redondo, Esther / Calvo, Guillermo / Radau, Sonja / Pardo, Mercedes / Gil, Concha / Monteoliva, Lucía

Microbiology spectrum

2022 Volume 10, Issue 3, Page(s) e0069822

Abstract: Candida albicans is the principal causative agent of lethal fungal infections, predominantly in immunocompromised hosts. Extracellular vesicles (EVs) have been described as crucial in the interaction of microorganisms with their host. Since the yeast-to- ... ...

Abstract	Candida albicans is the principal causative agent of lethal fungal infections, predominantly in immunocompromised hosts. Extracellular vesicles (EVs) have been described as crucial in the interaction of microorganisms with their host. Since the yeast-to-hypha transition is an important virulence trait with great impact in invasive candidiasis (IC), we have addressed the characterization of EVs secreted by hyphal cells (HEVs) from C. albicans, comparing them to yeast EVs (YEVs). YEVs comprised a larger population of bigger EVs with mainly cell wall proteins, while HEVs were smaller, in general, and had a much higher protein diversity. YEVs were able to rescue the sensitivity of a cell wall mutant against calcofluor white, presumably due to the larger amount of cell wall proteins they contained. On the other hand, HEVs also contained many cytoplasmic proteins related to protein metabolism and intracellular protein transport and the endosomal sorting complexes required for transport (ESCRT) pathway related to exosome biogenesis, pointing to an intracellular origin of HEVs. Interestingly, an active 20S proteasome complex was secreted exclusively in HEVs. Moreover, HEVs contained a greater number of virulence-related proteins. As for their immunogenic role, both types of EV presented immune reactivity with human sera from patients suffering invasive candidiasis; however, under our conditions, only HEVs showed a cytotoxic effect on human macrophages and could elicit the release of tumor necrosis factor alpha (TNF-α) by these macrophages.
MeSH term(s)	Candida albicans/metabolism ; Candidiasis ; Candidiasis, Invasive/metabolism ; Extracellular Vesicles/metabolism ; Fungal Proteins/metabolism ; Humans ; Hyphae/metabolism ; Immunity ; Proteasome Endopeptidase Complex/metabolism ; Tumor Necrosis Factor-alpha
Chemical Substances	Fungal Proteins ; Tumor Necrosis Factor-alpha ; Proteasome Endopeptidase Complex (EC 3.4.25.1)
Language	English
Publishing date	2022-05-23
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2807133-5
ISSN	2165-0497 ; 2165-0497
ISSN (online)	2165-0497
ISSN	2165-0497
DOI	10.1128/spectrum.00698-22
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: The external face of Candida albicans: A proteomic view of the cell surface and the extracellular environment.

Gil-Bona, Ana / Amador-García, Ahinara / Gil, Concha / Monteoliva, Lucia

Journal of proteomics

2017 Volume 180, Page(s) 70–79

Abstract: The cell surface and secreted proteins are the initial points of contact between Candida albicans and the host. Improvements in protein extraction approaches and mass spectrometers have allowed researchers to obtain a comprehensive knowledge of these ... ...

Abstract	The cell surface and secreted proteins are the initial points of contact between Candida albicans and the host. Improvements in protein extraction approaches and mass spectrometers have allowed researchers to obtain a comprehensive knowledge of these external subproteomes. In this paper, we review the published proteomic studies that have examined C. albicans extracellular proteins, including the cell surface proteins or surfome and the secreted proteins or secretome. The use of different approaches to isolate cell wall and cell surface proteins, such as fractionation approaches or cell shaving, have resulted in different outcomes. Proteins with N-terminal signal peptide, known as classically secreted proteins, and those that lack the signal peptide, known as unconventionally secreted proteins, have been consistently identified. Existing studies on C. albicans extracellular vesicles reveal that they are relevant as an unconventional pathway of protein secretion and can help explain the presence of proteins without a signal peptide, including some moonlighting proteins, in the cell wall and the extracellular environment. According to the global view presented in this review, cell wall proteins, virulence factors such as adhesins or hydrolytic enzymes, metabolic enzymes and stress related-proteins are important groups of proteins in C. albicans surfome and secretome. Biological significance: Candida albicans extracellular proteins are involved in biofilm formation, cell nutrient acquisition and cell wall integrity maintenance. Furthermore, these proteins include virulence factors and immunogenic proteins. This review is of outstanding interest, not only because it extends knowledge of the C. albicans surface and extracellular proteins that could be related with pathogenesis, but also because it presents insights that may facilitate the future development of new antifungal drugs and vaccines and contributes to efforts to identify new biomarkers that can be employed to diagnose candidiasis. Here, we list more than 570 C. albicans proteins that have been identified in extracellular locations to deliver the most extensive catalogue of this type of proteins to date. Moreover, we describe 16 proteins detected at all locations analysed in the works revised. These proteins include the glycophosphatidylinositol (GPI)-anchored proteins Ecm33, Pga4 and Phr2 and unconventional secretory proteins such as Eft2, Eno1, Hsp70, Pdc11, Pgk1 and Tdh3. Furthermore, 13 of these 16 proteins are immunogenic and could represent a set of interesting candidates for biomarker discovery.
MeSH term(s)	Candida albicans/metabolism ; Candida albicans/pathogenicity ; Candidiasis/metabolism ; Cell Membrane/metabolism ; Cell Wall/metabolism ; Extracellular Vesicles/metabolism ; Fungal Proteins/metabolism ; Humans ; Protein Transport ; Proteomics ; Virulence Factors/metabolism
Chemical Substances	Fungal Proteins ; Virulence Factors
Language	English
Publishing date	2017-12-06
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2400835-7
ISSN	1876-7737 ; 1874-3919
ISSN (online)	1876-7737
ISSN	1874-3919
DOI	10.1016/j.jprot.2017.12.002
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Trends in microbial proteomics.

Gil, Concha / Monteoliva, Lucía

Journal of proteomics

2014 Volume 97, Page(s) 1–2

MeSH term(s)	Bacteria/genetics ; Bacteria/metabolism ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Proteome/genetics ; Proteome/metabolism ; Proteomics/methods ; Proteomics/trends
Chemical Substances	Bacterial Proteins ; Proteome
Language	English
Publishing date	2014-01-31
Publishing country	Netherlands
Document type	Editorial
ZDB-ID	2400835-7
ISSN	1876-7737 ; 1874-3919
ISSN (online)	1876-7737
ISSN	1874-3919
DOI	10.1016/j.jprot.2013.12.015
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Mass Spectrometry-Based Proteomic and Immunoproteomic Analyses of the

Vaz, Catarina / Pitarch, Aida / Gómez-Molero, Emilia / Amador-García, Ahinara / Weig, Michael / Bader, Oliver / Monteoliva, Lucía / Gil, Concha

Journal of fungi (Basel, Switzerland)

2021 Volume 7, Issue 7

Abstract: Invasive candidiasis (IC) is associated with high morbidity and mortality in hospitalized patients if not diagnosed early. Long-term use of central venous catheters is a predisposing factor for IC. Hyphal forms ... ...

Abstract	Invasive candidiasis (IC) is associated with high morbidity and mortality in hospitalized patients if not diagnosed early. Long-term use of central venous catheters is a predisposing factor for IC. Hyphal forms of
Language	English
Publishing date	2021-06-23
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2784229-0
ISSN	2309-608X ; 2309-608X
ISSN (online)	2309-608X
ISSN	2309-608X
DOI	10.3390/jof7070501
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Genotypic, proteomic, and phenotypic approaches to decipher the response to caspofungin and calcineurin inhibitors in clinical isolates of echinocandin-resistant Candida glabrata.

Ceballos-Garzon, Andres / Monteoliva, Lucia / Gil, Concha / Alvarez-Moreno, Carlos / Vega-Vela, Nelson E / Engelthaler, David M / Bowers, Jolene / Le Pape, Patrice / Parra-Giraldo, Claudia M

The Journal of antimicrobial chemotherapy

2021 Volume 77, Issue 3, Page(s) 585–597

Abstract: Background: Echinocandin resistance represents a great concern, as these drugs are recommended as first-line therapy for invasive candidiasis. Echinocandin resistance is conferred by mutations in FKS genes. Nevertheless, pathways are crucial for ... ...

Abstract	Background: Echinocandin resistance represents a great concern, as these drugs are recommended as first-line therapy for invasive candidiasis. Echinocandin resistance is conferred by mutations in FKS genes. Nevertheless, pathways are crucial for enabling tolerance, evolution, and maintenance of resistance. Therefore, understanding the biological processes and proteins involved in the response to caspofungin may provide clues indicating new therapeutic targets. Objectives: We determined the resistance mechanism and assessed the proteome response to caspofungin exposure. We then evaluated the phenotypic impact of calcineurin inhibition by FK506 and cephalosporine A (CsA) on caspofungin-resistant Candida glabrata isolates. Methods: Twenty-five genes associated with caspofungin resistance were analysed by NGS, followed by studies of the quantitative proteomic response to caspofungin exposure. Then, susceptibility testing of caspofungin in presence of FK506 and CsA was performed. The effects of calcineurin inhibitor/caspofungin combinations on heat stress (40°C), oxidative stress (0.2 and 0.4 mM menadione) and on biofilm formation (polyurethane catheter) were analysed. Finally, a Galleria mellonella model using blastospores (1 × 109 cfu/mL) was developed to evaluate the impact of the combinations on larval survival. Results: F659-del was found in the FKS2 gene of resistant strains. Proteomics data showed some up-regulated proteins are involved in cell-wall biosynthesis, response to stress and pathogenesis, some of them being members of calmodulin-calcineurin pathway. Therefore, the impact of calmodulin inhibition was explored. Calmodulin inhibition restored caspofungin susceptibility, decreased capacity to respond to stress conditions, and reduced biofilm formation and in vivo pathogenicity. Conclusions: Our findings confirm that calmodulin-calcineurin-Crz1 could provide a relevant target in life-threatening invasive candidiasis.
MeSH term(s)	Antifungal Agents/pharmacology ; Antifungal Agents/therapeutic use ; Calcineurin Inhibitors/pharmacology ; Calcineurin Inhibitors/therapeutic use ; Candida glabrata ; Candidiasis, Invasive/drug therapy ; Caspofungin/pharmacology ; Caspofungin/therapeutic use ; Drug Resistance, Fungal/genetics ; Echinocandins/pharmacology ; Echinocandins/therapeutic use ; Fungal Proteins/genetics ; Fungal Proteins/metabolism ; Microbial Sensitivity Tests ; Proteomics
Chemical Substances	Antifungal Agents ; Calcineurin Inhibitors ; Echinocandins ; Fungal Proteins ; Caspofungin (F0XDI6ZL63)
Language	English
Publishing date	2021-12-10
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	191709-2
ISSN	1460-2091 ; 0305-7453
ISSN (online)	1460-2091
ISSN	0305-7453
DOI	10.1093/jac/dkab454
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1197: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MO 124: Show issues

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Trk1-mediated potassium uptake contributes to cell-surface properties and virulence of Candida glabrata.

Llopis-Torregrosa, Vicent / Vaz, Catarina / Monteoliva, Lucia / Ryman, Kicki / Engstrom, Ylva / Gacser, Attila / Gil, Concha / Ljungdahl, Per O / Sychrová, Hana

Scientific reports

2019 Volume 9, Issue 1, Page(s) 7529

Abstract: The absence of high-affinity potassium uptake in Candida glabrata, the consequence of the deletion of the TRK1 gene encoding the sole potassium-specific transporter, has a pleiotropic effect. Here, we show that in addition to changes in basic ... ...

Abstract	The absence of high-affinity potassium uptake in Candida glabrata, the consequence of the deletion of the TRK1 gene encoding the sole potassium-specific transporter, has a pleiotropic effect. Here, we show that in addition to changes in basic physiological parameters (e.g., membrane potential and intracellular pH) and decreased tolerance to various cell stresses, the loss of high affinity potassium uptake also alters cell-surface properties, such as an increased hydrophobicity and adherence capacity. The loss of an efficient potassium uptake system results in diminished virulence as assessed by two insect host models, Drosophila melanogaster and Galleria mellonella, and experiments with macrophages. Macrophages kill trk1Δ cells more effectively than wild type cells. Consistently, macrophages accrue less damage when co-cultured with trk1Δ mutant cells compared to wild-type cells. We further show that low levels of potassium in the environment increase the adherence of C. glabrata cells to polystyrene and the propensity of C. glabrata cells to form biofilms.
MeSH term(s)	Animals ; Biofilms/growth & development ; Candida glabrata/genetics ; Candida glabrata/metabolism ; Candida glabrata/pathogenicity ; Cation Transport Proteins/genetics ; Cell Adhesion/physiology ; Cell Line ; Cell Membrane/metabolism ; Drosophila melanogaster/microbiology ; Gene Expression Regulation, Fungal/genetics ; Humans ; Hydrophobic and Hydrophilic Interactions ; Ion Transport ; Macrophages/immunology ; Membrane Potentials/physiology ; Moths/microbiology ; Potassium/metabolism ; Potassium-Hydrogen Antiporters/genetics ; Surface Properties ; THP-1 Cells ; Virulence/genetics
Chemical Substances	Cation Transport Proteins ; Potassium-Hydrogen Antiporters ; Trk1 protein, Candida albicans ; Potassium (RWP5GA015D)
Language	English
Publishing date	2019-05-17
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-019-43912-1
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: The Combination of Iron and Copper Increases Pathogenicity and Induces Proteins Related to the Main Virulence Factors in Clinical Isolates of

Vélez, Nórida / Monteoliva, Lucía / Sánchez-Quitian, Zilpa-Adriana / Amador-García, Ahinara / García-Rodas, Rocío / Ceballos-Garzón, Andrés / Gil, Concha / Escandón, Patricia / Zaragoza, Óscar / Parra-Giraldo, Claudia-Marcela

Journal of fungi (Basel, Switzerland)

2022 Volume 8, Issue 1

Abstract: In fungi, metals are associated with the expression of virulence factors. However, it is unclear whether the uptake of metals affects their pathogenicity. This study aimed to evaluate the effect of iron/copper in modulating pathogenicity and proteomic ... ...

Abstract	In fungi, metals are associated with the expression of virulence factors. However, it is unclear whether the uptake of metals affects their pathogenicity. This study aimed to evaluate the effect of iron/copper in modulating pathogenicity and proteomic response in two clinical isolates of Methods: In both isolates, the effect of 50 µM iron and 500 µM copper on pathogenicity, capsule induction, and melanin production was evaluated. We then performed a quantitative proteomic analysis of cytoplasmic extracts exposed to that combination. Finally, the effect on pathogenicity by iron and copper was evaluated in eight additional isolates. Results: In both isolates, the combination of iron and copper increased pathogenicity, capsule size, and melanin production. Regarding proteomic data, proteins with increased levels after iron and copper exposure were related to biological processes such as cell stress, vesicular traffic (Ap1, Vps35), cell wall structure (Och1, Ccr4, Gsk3), melanin biosynthesis (Hem15, Mln2), DNA repair (Chk1), protein transport (Mms2), SUMOylation (Uba2), and mitochondrial transport (Atm1). Increased pathogenicity by exposure to metal combination was also confirmed in 90% of the eight isolates. Conclusions: The combination of these metals enhances pathogenicity and increases the abundance of proteins related to the main virulence factors.
Language	English
Publishing date	2022-01-06
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2784229-0
ISSN	2309-608X ; 2309-608X
ISSN (online)	2309-608X
ISSN	2309-608X
DOI	10.3390/jof8010057
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Deciphering the Association among Pathogenicity, Production and Polymorphisms of Capsule/Melanin in Clinical Isolates of

Vélez, Nórida / Vega-Vela, Nelson / Muñoz, Marina / Gómez, Paola / Escandón, Patricia / Ramírez, Juan David / Zaragoza, Oscar / Monteoliva Diaz, Lucía / Parra-Giraldo, Claudia-Marcela

Journal of fungi (Basel, Switzerland)

2022 Volume 8, Issue 3

Abstract: Background: Cryptococcus neoformans: Methods: Genome classification of 29 clinical isolates of : Results: Eleven MLST sequence types (STs) were found, the most frequent being ST69 (: Conclusions: No clear patterns were found in the analysis of ... ...

Abstract	Background: Cryptococcus neoformans Methods: Genome classification of 29 clinical isolates of Results: Eleven MLST sequence types (STs) were found, the most frequent being ST69 ( Conclusions: No clear patterns were found in the analysis of the phenotype and genotype of
Language	English
Publishing date	2022-02-28
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2784229-0
ISSN	2309-608X ; 2309-608X
ISSN (online)	2309-608X
ISSN	2309-608X
DOI	10.3390/jof8030245
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

To top

Full text online

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito