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  1. Article ; Online: Direct Identification of Functional Amyloid Proteins by Label-Free Quantitative Mass Spectrometry.

    Danielsen, Heidi N / Hansen, Susan H / Herbst, Florian-Alexander / Kjeldal, Henrik / Stensballe, Allan / Nielsen, Per H / Dueholm, Morten S

    Biomolecules

    2017  Volume 7, Issue 3

    Abstract: Functional amyloids are important structural and functional components of many biofilms, yet our knowledge of these fascinating polymers is limited to a few examples for which the native amyloids have been isolated in pure form. Isolation of the ... ...

    Abstract Functional amyloids are important structural and functional components of many biofilms, yet our knowledge of these fascinating polymers is limited to a few examples for which the native amyloids have been isolated in pure form. Isolation of the functional amyloids from other cell components represents a major bottleneck in the search for new functional amyloid systems. Here we present a label-free quantitative mass spectrometry method that allows identification of amyloid proteins directly in cell lysates. The method takes advantage of the extreme structural stability and polymeric nature of functional amyloids and the ability of concentrated formic acid to depolymerize the amyloids. An automated data processing pipeline that provides a short list of amyloid protein candidates was developed based on an amyloid-specific sigmoidal abundance signature in samples treated with increasing concentrations of formic acid. The method was evaluated using the
    MeSH term(s) Amyloidogenic Proteins/chemistry ; Amyloidogenic Proteins/isolation & purification ; Bacterial Proteins/chemistry ; Bacterial Proteins/isolation & purification ; Biofilms ; Escherichia coli/metabolism ; Escherichia coli/physiology ; Mass Spectrometry/methods ; Protein Stability ; Pseudomonas/metabolism ; Pseudomonas/physiology
    Chemical Substances Amyloidogenic Proteins ; Bacterial Proteins
    Language English
    Publishing date 2017-08-04
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2701262-1
    ISSN 2218-273X ; 2218-273X
    ISSN (online) 2218-273X
    ISSN 2218-273X
    DOI 10.3390/biom7030058
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Patient-reported factors associated with early arrival for stroke treatment.

    Eddelien, Heidi S / Butt, Jawad H / Amtoft, André C / Nielsen, Nicholine S K / Jensen, Emilie S / Danielsen, Ida M K / Christensen, Thomas / Danielsen, Anne K / Hornnes, Nete / Kruuse, Christina

    Brain and behavior

    2021  Volume 11, Issue 8, Page(s) e2225

    Abstract: Objective: Timely evaluation and initiation of treatment is the key for improving stroke outcomes, although minimizing the time from symptom onset to the first contact with healthcare professionals remains a challenge. We aimed to identify patient- ... ...

    Abstract Objective: Timely evaluation and initiation of treatment is the key for improving stroke outcomes, although minimizing the time from symptom onset to the first contact with healthcare professionals remains a challenge. We aimed to identify patient-related factors associated with early hospital arrival.
    Materials and methods: In this cross-sectional survey, we included patients with stroke or transient ischemic attack admitted directly to one of two noncomprehensive stroke units or transferred to the units from comprehensive stroke centers in the Capital Region of Denmark. Patient-reported factors associated with early hospital arrival were analyzed using multivariable logistic regression analysis adjusted for age, sex, education, living arrangement, brain location of the stroke, stroke severity, patient-perceived symptom severity, history of prior stroke, stroke risk factors, and knowledge of stroke symptoms.
    Results: In total, 479 patients with acute stroke were included (median age 74 (25th-75th percentile, 64-80), 40% women), of whom 46.4% arrived within 180 min of symptom onset. Factors associated with early hospital arrival were patients or bystanders choosing emergency medical service (EMS) for the first contact with a medical professional (adjusted odds ratio (OR), 3.41; 95% confidence interval, CI [1.57, 7.35]) or the patient's perceived symptom severity above the median score of 25 on a 100-point verbal scale (adjusted OR, 2.44; 95% CI [1.57, 3.82]). Living alone reduced the likelihood of early arrival (adjusted OR, 0.53; 95% CI [0.33, 0.86]).
    Conclusions: Only when patients perceived symptoms as severe or when EMS was selected as the first contact, early arrival for stroke treatment was ensured.
    MeSH term(s) Aged ; Cross-Sectional Studies ; Emergency Medical Services ; Female ; Humans ; Ischemic Attack, Transient ; Male ; Patient Reported Outcome Measures ; Stroke/therapy ; Time Factors
    Language English
    Publishing date 2021-06-04
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2623587-0
    ISSN 2162-3279 ; 2162-3279
    ISSN (online) 2162-3279
    ISSN 2162-3279
    DOI 10.1002/brb3.2225
    Database MEDical Literature Analysis and Retrieval System OnLINE

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