Article ; Online: Proximity Labeling for the Identification of Coronavirus-Host Protein Interactions.
Methods in molecular biology (Clifton, N.J.)
2020 Volume 2203, Page(s) 187–204
Abstract: Biotin-based proximity labeling circumvents major pitfalls of classical biochemical approaches to identify protein-protein interactions. It consists of enzyme-catalyzed biotin tags ubiquitously apposed on proteins located in close proximity of the ... ...
Abstract | Biotin-based proximity labeling circumvents major pitfalls of classical biochemical approaches to identify protein-protein interactions. It consists of enzyme-catalyzed biotin tags ubiquitously apposed on proteins located in close proximity of the labeling enzyme, followed by affinity purification and identification of biotinylated proteins by mass spectrometry. Here we outline the methods by which the molecular microenvironment of the coronavirus replicase/transcriptase complex (RTC), i.e., proteins located within a close perimeter of the RTC, can be determined by different proximity labeling approaches using BirA |
---|---|
MeSH term(s) | Animals ; Ascorbate Peroxidases/genetics ; Biotinylation ; Carbon-Nitrogen Ligases/genetics ; Cell Line ; Coronavirus/genetics ; Coronavirus/pathogenicity ; Enzymes/genetics ; Enzymes/metabolism ; Escherichia coli Proteins/genetics ; Fluorescent Antibody Technique ; Host-Pathogen Interactions/physiology ; Microorganisms, Genetically-Modified ; Proteomics/methods ; Repressor Proteins/genetics ; Viral Proteins/chemistry ; Viral Proteins/genetics ; Viral Proteins/metabolism |
Chemical Substances | Enzymes ; Escherichia coli Proteins ; Repressor Proteins ; Viral Proteins ; Ascorbate Peroxidases (EC 1.11.1.11) ; Carbon-Nitrogen Ligases (EC 6.3.-) ; birA protein, E coli (EC 6.3.4.15) |
Keywords | covid19 |
Language | English |
Publishing date | 2020-07-29 |
Publishing country | United States |
Document type | Journal Article |
ISSN | 1940-6029 |
ISSN (online) | 1940-6029 |
DOI | 10.1007/978-1-0716-0900-2_14 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
Full text online
More links
Kategorien
Order via subito
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.
Inter-library loan at ZB MED
Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.