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  1. Article ; Online: Changing faces of stress: Impact of heat and arsenite treatment on the composition of stress granules.

    Frydrýšková, Klára / Mašek, Tomáš / Pospíšek, Martin

    Wiley interdisciplinary reviews. RNA

    2020  Volume 11, Issue 6, Page(s) e1596

    Abstract: Stress granules (SGs), hallmarks of the cellular adaptation to stress, promote survival, conserve cellular energy, and are fully dissolved upon the cessation of stress treatment. Different stresses can initiate the assembly of SGs, but arsenite and heat ... ...

    Abstract Stress granules (SGs), hallmarks of the cellular adaptation to stress, promote survival, conserve cellular energy, and are fully dissolved upon the cessation of stress treatment. Different stresses can initiate the assembly of SGs, but arsenite and heat are the best studied of these stresses. The composition of SGs and posttranslational modifications of SG proteins differ depending on the type and severity of the stress insult, methodology used, cell line, and presence of overexpressed and tagged proteins. A group of 18 proteins showing differential localization to SGs in heat- and arsenite-stressed mammalian cell lines is described. Upon severe and prolonged stress, physiological SGs transform into more solid protein aggregates that are no longer reversible and do not contain mRNA. Similar pathological inclusions are hallmarks of neurodegenerative diseases. SGs induced by heat stress are less dynamic than SGs induced by arsenite and contain a set of unique proteins and linkage-specific polyubiquitinated proteins. The same types of ubiquitin linkages have been found to contribute to the development of neurodegenerative disorders such as Parkinson disease, Alzheimer disease, and amyotrophic lateral sclerosis (ALS). We propose heat stress-induced SGs as a possible model of an intermediate stage along the transition from dynamic, fully reversible arsenite stress-induced SGs toward aberrant SGs, the hallmark of neurodegenerative diseases. Stress- and methodology-specific differences in the compositions of SGs and the transition of SGs to aberrant protein aggregates are discussed. This article is categorized under: RNA in Disease and Development > RNA in Disease RNA Interactions with Proteins and Other Molecules > RNA-Protein Complexes RNA Export and Localization > RNA Localization.
    MeSH term(s) Animals ; Arsenites/metabolism ; Cytoplasmic Granules/metabolism ; Hot Temperature ; Humans ; Protein Processing, Post-Translational ; Stress, Physiological
    Chemical Substances Arsenites
    Language English
    Publishing date 2020-05-03
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2634714-3
    ISSN 1757-7012 ; 1757-7004
    ISSN (online) 1757-7012
    ISSN 1757-7004
    DOI 10.1002/wrna.1596
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 6953: Show issues Location:
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  2. Article ; Online: Major splice variants and multiple polyadenylation site utilization in mRNAs encoding human translation initiation factors eIF4E1 and eIF4E3 regulate the translational regulators?

    Mrvová, Silvia / Frydrýšková, Klára / Pospíšek, Martin / Vopálenský, Václav / Mašek, Tomáš

    Molecular genetics and genomics : MGG

    2018  Volume 293, Issue 1, Page(s) 167–186

    Abstract: Alternative polyadenylation is an important and pervasive mechanism that generates heterogeneous 3'-termini of mRNA and is considered an important regulator of gene expression. We performed bioinformatics analyses of ESTs and the 3'-UTRs of the main ... ...

    Abstract Alternative polyadenylation is an important and pervasive mechanism that generates heterogeneous 3'-termini of mRNA and is considered an important regulator of gene expression. We performed bioinformatics analyses of ESTs and the 3'-UTRs of the main transcript splice variants of the translational initiation factor eIF4E1 and its family members, eIF4E2 and eIF4E3. This systematic analysis led to the prediction of new polyadenylation signals. All identified polyadenylation sites were subsequently verified by 3'RACE of transcripts isolated from human lymphoblastic cell lines. This led to the observation that multiple simultaneous polyadenylation site utilization occurs in single cell population. Importantly, we described the use of new polyadenylation site in the eIF4E1 mRNA, which lacked any known polyadenylation signal. The proportion of eIF4E1 transcripts derived from the first two polyadenylation sites in eIF4E1 mRNA achieved 15% in a wide range of cell lines. This result demonstrates the ubiquitous presence of ARE-lacking transcripts, which escape HuR/Auf1-mediated control, the main mechanism of eIF4E1 gene expression regulation. We found many EST clones documenting the significant production of transcript variants 2-4 of eIF4E2 gene that encode proteins with C-termini that were distinct from the mainly studied prototypical isoform A. Similarly, eIF4E3 mRNAs are produced as two main variants with the same very long 3'-UTR with potential for heavy post-transcriptional regulation. We identified sparsely documented transcript variant 1 of eIF4E3 gene in human placenta. eIF4E3 truncated transcript variants were found mainly in brain. We propose to elucidate the minor splice variants of eIF4E2 and eIF4E3 in great detail because they might produce proteins with modified features that fulfill different cellular roles from their major counterparts.
    MeSH term(s) 3' Untranslated Regions ; Brain/metabolism ; Cell Line ; Eukaryotic Initiation Factor-4E/genetics ; Eukaryotic Initiation Factor-4E/metabolism ; Expressed Sequence Tags ; Female ; Gene Expression Regulation ; Humans ; Placenta/metabolism ; Polyadenylation/genetics ; Pregnancy ; RNA Cap-Binding Proteins/genetics ; RNA Splicing/genetics ; RNA, Messenger/genetics ; RNA, Messenger/metabolism
    Chemical Substances 3' Untranslated Regions ; EIF4E2 protein, human ; Eukaryotic Initiation Factor-4E ; RNA Cap-Binding Proteins ; RNA, Messenger ; eIF4E3 protein, human
    Language English
    Publishing date 2018-02
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2044817-X
    ISSN 1617-4623 ; 1617-4615
    ISSN (online) 1617-4623
    ISSN 1617-4615
    DOI 10.1007/s00438-017-1375-4
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1198: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  3. Article: Major splice variants and multiple polyadenylation site utilization in mRNAs encoding human translation initiation factors eIF4E1 and eIF4E3 regulate the translational regulators?

    Mrvová, Silvia / Klára Frydrýšková / Martin Pospíšek / Václav Vopálenský / Tomáš Mašek

    Molecular genetics and genomics. 2018 Feb., v. 293, no. 1

    2018  

    Abstract: Alternative polyadenylation is an important and pervasive mechanism that generates heterogeneous 3′-termini of mRNA and is considered an important regulator of gene expression. We performed bioinformatics analyses of ESTs and the 3′-UTRs of the main ... ...

    Abstract Alternative polyadenylation is an important and pervasive mechanism that generates heterogeneous 3′-termini of mRNA and is considered an important regulator of gene expression. We performed bioinformatics analyses of ESTs and the 3′-UTRs of the main transcript splice variants of the translational initiation factor eIF4E1 and its family members, eIF4E2 and eIF4E3. This systematic analysis led to the prediction of new polyadenylation signals. All identified polyadenylation sites were subsequently verified by 3′RACE of transcripts isolated from human lymphoblastic cell lines. This led to the observation that multiple simultaneous polyadenylation site utilization occurs in single cell population. Importantly, we described the use of new polyadenylation site in the eIF4E1 mRNA, which lacked any known polyadenylation signal. The proportion of eIF4E1 transcripts derived from the first two polyadenylation sites in eIF4E1 mRNA achieved 15% in a wide range of cell lines. This result demonstrates the ubiquitous presence of ARE-lacking transcripts, which escape HuR/Auf1-mediated control, the main mechanism of eIF4E1 gene expression regulation. We found many EST clones documenting the significant production of transcript variants 2–4 of eIF4E2 gene that encode proteins with C-termini that were distinct from the mainly studied prototypical isoform A. Similarly, eIF4E3 mRNAs are produced as two main variants with the same very long 3′-UTR with potential for heavy post-transcriptional regulation. We identified sparsely documented transcript variant 1 of eIF4E3 gene in human placenta. eIF4E3 truncated transcript variants were found mainly in brain. We propose to elucidate the minor splice variants of eIF4E2 and eIF4E3 in great detail because they might produce proteins with modified features that fulfill different cellular roles from their major counterparts.
    Keywords bioinformatics ; brain ; clones ; expressed sequence tags ; gene expression regulation ; genes ; human cell lines ; humans ; messenger RNA ; placenta ; prediction ; proteins ; translation (genetics)
    Language English
    Dates of publication 2018-02
    Size p. 167-186.
    Publishing place Springer Berlin Heidelberg
    Document type Article
    ISSN 1617-4615
    DOI 10.1007/s00438-017-1375-4
    Database NAL-Catalogue (AGRICOLA)

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  4. Article ; Online: Distinct recruitment of human eIF4E isoforms to processing bodies and stress granules.

    Frydryskova, Klara / Masek, Tomas / Borcin, Katerina / Mrvova, Silvia / Venturi, Veronica / Pospisek, Martin

    BMC molecular biology

    2016  Volume 17, Issue 1, Page(s) 21

    Abstract: Background: Eukaryotic translation initiation factor 4E (eIF4E) plays a pivotal role in the control of cap-dependent translation initiation, modulates the fate of specific mRNAs, occurs in processing bodies (PBs) and is required for formation of stress ... ...

    Abstract Background: Eukaryotic translation initiation factor 4E (eIF4E) plays a pivotal role in the control of cap-dependent translation initiation, modulates the fate of specific mRNAs, occurs in processing bodies (PBs) and is required for formation of stress granules (SGs). In this study, we focused on the subcellular localization of a representative compendium of eIF4E protein isoforms, particularly on the less studied members of the human eIF4E protein family, eIF4E2 and eIF4E3.
    Results: We showed that unlike eIF4E1, its less studied isoform eIF4E3_A, encoded by human chromosome 3, localized to stress granules but not PBs upon both heat shock and arsenite stress. Furthermore, we found that eIF4E3_A interacts with human translation initiation factors eIF4G1, eIF4G3 and PABP1 in vivo and sediments into the same fractions as canonical eIF4E1 during polysome analysis in sucrose gradients. Contrary to this finding, the truncated human eIF4E3 isoform, eIF4E3_B, showed no localization to SGs and no binding to eIF4G. We also highlighted that eIF4E2 may exhibit distinct functions under different stresses as it readily localizes to P-bodies during arsenite and heat stresses, whereas it is redirected to stress granules only upon heat shock. We extended our study to a number of protein variants, arising from alternative mRNA splicing, of each of the three eIF4E isoforms. Our results surprisingly uncovered differences in the ability of eIF4E1_1 and eIF4E1_3 to form stress granules in response to cellular stresses.
    Conclusion: Our comparison of all three human eIF4E isoforms and their protein variants enriches the intriguing spectrum of roles attributed to the eukaryotic initiation translation factors of the 4E family, which exhibit a distinctive localization within different RNA granules under different stresses. The localization of eIF4E3_A to stress granules, but not to processing bodies, along with its binding to eIF4G and PABP1 suggests a role of human eIF4E3_A in translation initiation rather than its involvement in a translational repression and mRNA decay and turnover. The localization of eIF4E2 to stress granules under heat shock but not arsenite stress indicates its distinct function in cellular response to these stresses and points to the variable protein content of SGs as a consequence of different stress insults.
    MeSH term(s) Amino Acid Sequence ; Cell Line ; Cloning, Molecular ; Cytosol/metabolism ; Eukaryotic Initiation Factor-4E/analysis ; Eukaryotic Initiation Factor-4E/genetics ; Eukaryotic Initiation Factor-4E/metabolism ; HEK293 Cells ; Heat-Shock Response ; Humans ; Oxidative Stress ; Poly(A)-Binding Protein I/analysis ; Poly(A)-Binding Protein I/metabolism ; RNA Cap-Binding Proteins/analysis ; RNA Cap-Binding Proteins/genetics ; RNA Cap-Binding Proteins/metabolism ; RNA, Messenger/genetics ; Sequence Alignment
    Chemical Substances EIF4E2 protein, human ; Eukaryotic Initiation Factor-4E ; Poly(A)-Binding Protein I ; RNA Cap-Binding Proteins ; RNA, Messenger ; eIF4E3 protein, human
    Language English
    Publishing date 2016-08-30
    Publishing country England
    Document type Journal Article
    ISSN 1471-2199
    ISSN (online) 1471-2199
    DOI 10.1186/s12867-016-0072-x
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