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  1. Article: Prävention und Therapie von Staphylokokkeninfektionen. Übersicht von M. Aepfelbacher, Hamburg

    Aepfelbacher, M.

    MMW-Fortschritte der Medizin

    2005  Volume 147, Issue 4, Page(s) 33

    Language German
    Document type Article
    ZDB-ID 1478211-x
    ISSN 1438-3276
    Database Current Contents Medicine

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    In stock of ZB MED Cologne/Königswinter

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  2. Article ; Online: [No title information]

    Carsten, Alexander / Failla, Antonio Virgilio / Aepfelbacher, Martin

    Journal of microscopy

    2024  

    Abstract: Since its introduction in 2017, MINFLUX nanoscopy has shown that it can visualise fluorescent molecules with an exceptional localisation precision of a few nanometres. In this overview, we provide a brief insight into technical implementations, ... ...

    Title translation MINFLUX nanoscopy: Visualising biological matter at the nanoscale level.
    Abstract Since its introduction in 2017, MINFLUX nanoscopy has shown that it can visualise fluorescent molecules with an exceptional localisation precision of a few nanometres. In this overview, we provide a brief insight into technical implementations, fluorescent marker developments and biological studies that have been conducted in connection with MINFLUX imaging and tracking. We also formulate ideas on how MINFLUX nanoscopy and derived technologies could influence bioimaging in the future. This insight is intended as a general starting point for an audience looking for a brief overview of MINFLUX nanoscopy from theory to application.
    Language Italian
    Publishing date 2024-04-25
    Publishing country England
    Document type English Abstract ; Journal Article
    ZDB-ID 219263-9
    ISSN 1365-2818 ; 0022-2720
    ISSN (online) 1365-2818
    ISSN 0022-2720
    DOI 10.1111/jmi.13306
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Super-resolution fluorescence microscopy for investigating bacterial cell biology.

    Carsten, Alexander / Wolters, Manuel / Aepfelbacher, Martin

    Molecular microbiology

    2023  Volume 121, Issue 4, Page(s) 646–658

    Abstract: Super-resolution fluorescence microscopy technologies developed over the past two decades have pushed the resolution limit for fluorescently labeled molecules into the nanometer range. These technologies have the potential to study bacterial structures, ... ...

    Abstract Super-resolution fluorescence microscopy technologies developed over the past two decades have pushed the resolution limit for fluorescently labeled molecules into the nanometer range. These technologies have the potential to study bacterial structures, for example, macromolecular assemblies such as secretion systems, with single-molecule resolution on a millisecond time scale. Here we review recent applications of super-resolution fluorescence microscopy with a focus on bacterial secretion systems. We also describe MINFLUX fluorescence nanoscopy, a relatively new technique that promises to one day produce molecular movies of molecular machines in action.
    MeSH term(s) Microscopy, Fluorescence/methods ; Bacteria ; Nanotechnology/methods
    Language English
    Publishing date 2023-12-01
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 619315-8
    ISSN 1365-2958 ; 0950-382X
    ISSN (online) 1365-2958
    ISSN 0950-382X
    DOI 10.1111/mmi.15203
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Live imaging of Yersinia translocon formation and immune recognition in host cells.

    Rudolph, Maren / Carsten, Alexander / Kulnik, Susanne / Aepfelbacher, Martin / Wolters, Manuel

    PLoS pathogens

    2022  Volume 18, Issue 5, Page(s) e1010251

    Abstract: Yersinia enterocolitica employs a type three secretion system (T3SS) to translocate immunosuppressive effector proteins into host cells. To this end, the T3SS assembles a translocon/pore complex composed of the translocator proteins YopB and YopD in host ...

    Abstract Yersinia enterocolitica employs a type three secretion system (T3SS) to translocate immunosuppressive effector proteins into host cells. To this end, the T3SS assembles a translocon/pore complex composed of the translocator proteins YopB and YopD in host cell membranes serving as an entry port for the effectors. The translocon is formed in a Yersinia-containing pre-phagosomal compartment that is connected to the extracellular space. As the phagosome matures, the translocon and the membrane damage it causes are recognized by the cell-autonomous immune system. We infected cells in the presence of fluorophore-labeled ALFA-tag-binding nanobodies with a Y. enterocolitica strain expressing YopD labeled with an ALFA-tag. Thereby we could record the integration of YopD into translocons and its intracellular fate in living host cells. YopD was integrated into translocons around 2 min after uptake of the bacteria into a phosphatidylinositol-4,5-bisphosphate enriched pre-phagosomal compartment and remained there for 27 min on average. Damaging of the phagosomal membrane as visualized with recruitment of GFP-tagged galectin-3 occurred in the mean around 14 min after translocon formation. Shortly after recruitment of galectin-3, guanylate-binding protein 1 (GBP-1) was recruited to phagosomes, which was accompanied by a decrease in the signal intensity of translocons, suggesting their degradation or disassembly. In sum, we were able for the first time to film the spatiotemporal dynamics of Yersinia T3SS translocon formation and degradation and its sensing by components of the cell-autonomous immune system.
    MeSH term(s) Bacterial Outer Membrane Proteins/metabolism ; Bacterial Proteins/metabolism ; Galectin 3 ; Type III Secretion Systems/metabolism ; Yersinia/metabolism ; Yersinia pseudotuberculosis/metabolism
    Chemical Substances Bacterial Outer Membrane Proteins ; Bacterial Proteins ; Galectin 3 ; Type III Secretion Systems
    Language English
    Publishing date 2022-05-23
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2205412-1
    ISSN 1553-7374 ; 1553-7374
    ISSN (online) 1553-7374
    ISSN 1553-7374
    DOI 10.1371/journal.ppat.1010251
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: First case of bloodstream infection caused by

    Both, Anna / Huang, Jiabin / Wenzel, Philipp / Aepfelbacher, Martin / Rohde, Holger / Christner, Martin / Hentschke, Moritz

    New microbes and new infections

    2023  Volume 53, Page(s) 101117

    Abstract: Members of ... ...

    Abstract Members of the
    Language English
    Publishing date 2023-03-28
    Publishing country England
    Document type Journal Article
    ZDB-ID 2750179-6
    ISSN 2052-2975
    ISSN 2052-2975
    DOI 10.1016/j.nmni.2023.101117
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Adaptation and validation of a quantitative vanA/vanB DNA screening assay on a high-throughput PCR system.

    Giersch, Katja / Tanida, Konstantin / Both, Anna / Nörz, Dominik / Heim, Denise / Rohde, Holger / Aepfelbacher, Martin / Lütgehetmann, Marc

    Scientific reports

    2024  Volume 14, Issue 1, Page(s) 3523

    Abstract: Vancomycin resistant enterococci (VRE) are a leading cause of ICU-acquired bloodstream infections in Europe. The bacterial load in enteral colonization may be associated with a higher probability of transmission. Here, we aimed to establish a ... ...

    Abstract Vancomycin resistant enterococci (VRE) are a leading cause of ICU-acquired bloodstream infections in Europe. The bacterial load in enteral colonization may be associated with a higher probability of transmission. Here, we aimed to establish a quantitative vanA/vanB DNA real-time PCR assay on a high-throughput system. Limits of detection (LOD), linear range and precision were determined using serial bacterial dilutions. LOD was 46.9 digital copies (dcp)/ml for vanA and 60.8 dcp/ml for vanB. The assay showed excellent linearity between 4.7 × 10
    MeSH term(s) Humans ; Vancomycin-Resistant Enterococci/genetics ; Predictive Value of Tests ; Real-Time Polymerase Chain Reaction ; DNA ; DNA, Bacterial/genetics ; DNA, Bacterial/analysis ; Bacterial Proteins/genetics ; Gram-Positive Bacterial Infections/microbiology ; Anti-Bacterial Agents
    Chemical Substances DNA (9007-49-2) ; DNA, Bacterial ; Bacterial Proteins ; Anti-Bacterial Agents
    Language English
    Publishing date 2024-02-12
    Publishing country England
    Document type Journal Article
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-024-54037-5
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: A bacterial effector protein promotes nuclear translocation of Stat3 to induce IL-10.

    Berneking, Laura / Bekere, Indra / Rob, Sören / Schnapp, Marie / Huang, Jiabin / Ruckdeschel, Klaus / Aepfelbacher, Martin

    European journal of cell biology

    2023  Volume 102, Issue 4, Page(s) 151364

    Abstract: The multifunctional Yersinia effector YopM inhibits effector triggered immunity and increases production of the anti-inflammatory cytokine Interleukin-10 (IL-10) to suppress the host immune response. Previously it was shown that YopM induces IL-10 gene ... ...

    Abstract The multifunctional Yersinia effector YopM inhibits effector triggered immunity and increases production of the anti-inflammatory cytokine Interleukin-10 (IL-10) to suppress the host immune response. Previously it was shown that YopM induces IL-10 gene expression by elevating phosphorylation of the serine-threonine kinase RSK1 in the nucleus of human macrophages. Using transcriptomics, we found that YopM strongly affects expression of genes belonging to the JAK-STAT signaling pathway. Further analysis revealed that YopM mediates nuclear translocation of the transcription factor Stat3 in Y. enterocolitica infected macrophages and that knockdown of Stat3 inhibited YopM-induced IL-10 gene expression. YopM-induced Stat3 translocation did not depend on autocrine IL-10, activation of RSK1 or tyrosine phosphorylation of Stat3. Thus, besides activation of RSK1, stimulation of nuclear translocation of Stat3 is another mechanism by which YopM increases IL-10 gene expression in macrophages.
    MeSH term(s) Humans ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Interleukin-10/genetics ; Interleukin-10/metabolism ; Bacterial Outer Membrane Proteins/genetics ; Bacterial Outer Membrane Proteins/metabolism ; Macrophages/metabolism ; Gene Expression Regulation ; STAT3 Transcription Factor/genetics ; STAT3 Transcription Factor/metabolism ; Phosphorylation
    Chemical Substances Bacterial Proteins ; Interleukin-10 (130068-27-8) ; Bacterial Outer Membrane Proteins ; STAT3 Transcription Factor
    Language English
    Publishing date 2023-10-02
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 391967-5
    ISSN 1618-1298 ; 0070-2463 ; 0171-9335
    ISSN (online) 1618-1298
    ISSN 0070-2463 ; 0171-9335
    DOI 10.1016/j.ejcb.2023.151364
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: The Impact of Antimicrobial Therapy Duration in the Treatment of Prosthetic Joint Infections Depending on Surgical Strategies: A Systematic Review and Meta-analysis.

    Olearo, Flaminia / Zanichelli, Veronica / Exarchakou, Aimilia / Both, Anna / Uςkay, Ilker / Aepfelbacher, Martin / Rohde, Holger

    Open forum infectious diseases

    2023  Volume 10, Issue 5, Page(s) ofad246

    Abstract: The aim of this systematic review was to address the question if short antibiotic treatment (SAT; at least 4 but <12 weeks) versus long antibiotic treatment (LAT) affects outcomes in prosthetic joint infections (PJIs). Database research (Medline, Embase, ...

    Abstract The aim of this systematic review was to address the question if short antibiotic treatment (SAT; at least 4 but <12 weeks) versus long antibiotic treatment (LAT) affects outcomes in prosthetic joint infections (PJIs). Database research (Medline, Embase, Web of Science, Scopus, Cochrane) retrieved 3740 articles, of which 10 studies were included in the analysis. Compared to LAT, 11% lower odds of treatment failure in the SAT group were found, although the difference was not statistically significant (pooled odds ratio, 0.89 [95% confidence interval, .53-1.50]). No difference in treatment failure was found between SAT and LAT once stratified by type of surgery, studies conducted in the United States versus Europe, study design, and follow-up. There is still no conclusive evidence that antibiotic treatment of PJIs for 12 weeks or longer is associated with better outcomes, irrespective of the type of surgical procedure. Most recent, high-quality studies tend to favor longer antibiotic courses, making them preferable in most situations.
    Language English
    Publishing date 2023-05-07
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 2757767-3
    ISSN 2328-8957
    ISSN 2328-8957
    DOI 10.1093/ofid/ofad246
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Book ; Thesis: Fluoreszenzspektroskopische Untersuchungen über den Einfluß von 1,25-Dihydroxy-Vitamin D2 auf die zytosolische CA++-Konzentration in monozytoiden Zellen (U 937)

    Aepfelbacher, Martin

    1990  

    Author's details vorgelegt von Martin Aepfelbacher
    Size 79 S. : graph. Darst.
    Document type Book ; Thesis
    Thesis / German Habilitation thesis München, Univ., Diss., 1990
    HBZ-ID HT003823663
    Database Catalogue ZB MED Medicine, Health

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    Shelf markLoan statusLocation
    91 D 2707 order for loan Magazin

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  10. Article ; Online: Structural basis to repurpose boron-based proteasome inhibitors Bortezomib and Ixazomib as β-lactamase inhibitors.

    Perbandt, Markus / Werner, Nadine / Prester, Andreas / Rohde, Holger / Aepfelbacher, Martin / Hinrichs, Winfried / Betzel, Christian

    Scientific reports

    2022  Volume 12, Issue 1, Page(s) 5510

    Abstract: ... of the β-lactamase CTX-M-14 with covalently bound Bortezomib and Ixazomib at high resolutions of 1.3 and 1 ... We further investigate differences in binding of Bortezomib/Ixazomib to CTX-M-14 and its target proteases ...

    Abstract β-lactamases are a major cause of rapidly emerging and spreading antibiotic resistance. Currently β-lactamase inhibitors (BLIs) in clinical use act only on Ambler Class A, C and some class D lactamases. The urgent need to identify new BLIs recently lead to FDA approval of boron-based compounds BLIs, e.g. Vaborbactam. The boron-based proteasome inhibitors Bortezomib and Ixazomib are used in cancer therapy as multiple myeloma drugs but they also bind to Ser-/Thr- proteases. In this study we show the crystal structures of the β-lactamase CTX-M-14 with covalently bound Bortezomib and Ixazomib at high resolutions of 1.3 and 1.1 Å, respectively. Ixazomib is well defined in electron density whereas Bortezomib show some disorder which corresponds to weaker inhibition efficiency observed for Ixazomib. Both inhibitors mimic the deacylation transition state of β-lactam hydrolysis, because they replace the deacylating water molecule. We further investigate differences in binding of Bortezomib/Ixazomib to CTX-M-14 and its target proteases as well as known β-lactamase drugs. Our findings can help to use Bortezomib/Ixazomib as lead compounds for development of new BLIs.
    MeSH term(s) Boron ; Boron Compounds ; Bortezomib/pharmacology ; Bortezomib/therapeutic use ; Glycine/analogs & derivatives ; Proteasome Inhibitors/pharmacology ; beta-Lactamase Inhibitors/pharmacology
    Chemical Substances Boron Compounds ; Proteasome Inhibitors ; beta-Lactamase Inhibitors ; Bortezomib (69G8BD63PP) ; ixazomib (71050168A2) ; Boron (N9E3X5056Q) ; Glycine (TE7660XO1C)
    Language English
    Publishing date 2022-04-01
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-022-09392-6
    Database MEDical Literature Analysis and Retrieval System OnLINE

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