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  1. Article ; Online: Challenges associated with the development of scientific projects and studies in digital health and mobile technologies.

    Valerio Netto, Antonio / Salvador, Maria Elisabete

    Revista brasileira de enfermagem

    2020  Volume 73, Issue 6, Page(s) e73n6

    MeSH term(s) Humans ; Research ; Technology ; Telemedicine
    Language Spanish
    Publishing date 2020-09-21
    Publishing country Brazil
    Document type Editorial ; Comment
    ZDB-ID 731983-6
    ISSN 1984-0446 ; 0034-7167
    ISSN (online) 1984-0446
    ISSN 0034-7167
    DOI 10.1590/0034-7167.202073n601
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Depression and Health-Related Quality of Life Among Young Adult Breast Cancer Patients: The Mediating Role of Reproductive Concerns.

    Bártolo, Ana / Santos, Isabel M / Valério, Elisabete / Monteiro, Sara

    Journal of adolescent and young adult oncology

    2020  Volume 9, Issue 3, Page(s) 431–435

    Abstract: Biological motherhood plays an important role in the lives of many young women facing breast cancer and threats to reproduction may be disruptive. In this study, we explored the indirect effects of the importance of parenthood and childlessness on ... ...

    Abstract Biological motherhood plays an important role in the lives of many young women facing breast cancer and threats to reproduction may be disruptive. In this study, we explored the indirect effects of the importance of parenthood and childlessness on depression and health-related quality of life (HRQoL) among cancer patients 18-40 years of age (
    MeSH term(s) Adolescent ; Adult ; Breast Neoplasms/psychology ; Depression/psychology ; Female ; Humans ; Quality of Life/psychology ; Reproduction/physiology ; Young Adult
    Language English
    Publishing date 2020-03-24
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2607978-1
    ISSN 2156-535X ; 2156-5333
    ISSN (online) 2156-535X
    ISSN 2156-5333
    DOI 10.1089/jayao.2019.0144
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Isolation and Characterization of

    Menezes, Carina / Valério, Elisabete / Botelho, Maria João / Dias, Elsa

    Toxins

    2020  Volume 12, Issue 1

    Abstract: In the summer of 2015, an intense cyanobacterial bloom producing geosmin/2-methylisoborneol (MIB) occurred in the Roxo freshwater reservoir in Alentejo, Portugal. The drinking water supplied from the Roxo water treatment plant (WTP) exhibited an ... ...

    Abstract In the summer of 2015, an intense cyanobacterial bloom producing geosmin/2-methylisoborneol (MIB) occurred in the Roxo freshwater reservoir in Alentejo, Portugal. The drinking water supplied from the Roxo water treatment plant (WTP) exhibited an unpleasant odor/taste and a significant cyanobacteria density was detected in the finished water at the exit of the WTP. Cyanobacteria were not evaluated downstream of the WTP, namely, at the city reservoir. The aim of this work was to isolate and characterize viable cyanobacteria present in finished water (exit of the WTP and city reservoir) that withstand conventional water treatment. Treated water samples collected at both sites were inoculated in Z8 culture medium to provide the conditions for putative cyanobacterial growth. After 30 days, filamentous cyanobacteria were observed in cultures inoculated with samples from the exit point of the WTP. Viable trichomes were isolated and identified as
    MeSH term(s) Bacterial Toxins/analysis ; Cylindrospermopsis/isolation & purification ; Cylindrospermopsis/physiology ; Drinking Water/microbiology ; Fresh Water/microbiology ; Portugal ; Saxitoxin ; Water Microbiology
    Chemical Substances Bacterial Toxins ; Drinking Water ; Saxitoxin (35523-89-8)
    Language English
    Publishing date 2020-01-08
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2518395-3
    ISSN 2072-6651 ; 2072-6651
    ISSN (online) 2072-6651
    ISSN 2072-6651
    DOI 10.3390/toxins12010040
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Microbial Source Tracking as a Method of Determination of Beach Sand Contamination.

    Valério, Elisabete / Santos, Maria Leonor / Teixeira, Pedro / Matias, Ricardo / Mendonça, João / Ahmed, Warish / Brandão, João

    International journal of environmental research and public health

    2022  Volume 19, Issue 13

    Abstract: Beach sand may act as a reservoir for numerous microorganisms, including enteric pathogens. Several of these pathogens originate in human or animal feces, which may pose a public health risk. In August 2019, high levels of fecal indicator bacteria (FIB) ... ...

    Abstract Beach sand may act as a reservoir for numerous microorganisms, including enteric pathogens. Several of these pathogens originate in human or animal feces, which may pose a public health risk. In August 2019, high levels of fecal indicator bacteria (FIB) were detected in the sand of the Azorean beach Prainha, Terceira Island, Portugal. Remediation measures were promptly implemented, including sand removal and the spraying of chlorine to restore the sand quality. To determine the source of the fecal contamination, during the first campaign, supratidal sand samples were collected from several sites along the beach, followed by microbial source tracking (MST) analyses of
    MeSH term(s) Animals ; Bacteria/classification ; Bacteria/genetics ; Bacteria/isolation & purification ; Bacteroides/genetics ; Bacteroides/isolation & purification ; Bathing Beaches/standards ; Charadriiformes ; Dogs ; Environmental Monitoring/methods ; Feces/microbiology ; Portugal ; Ruminants ; Sand/microbiology ; Water Microbiology ; Water Pollution/analysis
    Chemical Substances Sand
    Language English
    Publishing date 2022-06-28
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2175195-X
    ISSN 1660-4601 ; 1661-7827
    ISSN (online) 1660-4601
    ISSN 1661-7827
    DOI 10.3390/ijerph19137934
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Strategies for Monitoring Microbial Life in Beach Sand for Protection of Public Health.

    Brandão, João / Valério, Elisabete / Weiskerger, Chelsea / Veríssimo, Cristina / Sarioglou, Konstantina / Novak Babič, Monika / Solo-Gabriele, Helena M / Sabino, Raquel / Rebelo, Maria Teresa

    International journal of environmental research and public health

    2023  Volume 20, Issue 9

    Abstract: The 2021 revised guidelines of the World Health Organization recommend monitoring the quality of sand in addition to water at recreational beaches. This review provides background information about the types of beaches, the characteristics of sand, and ... ...

    Abstract The 2021 revised guidelines of the World Health Organization recommend monitoring the quality of sand in addition to water at recreational beaches. This review provides background information about the types of beaches, the characteristics of sand, and the microbiological parameters that should be measured. Analytical approaches are described for quantifying fungi and fecal indicator bacteria from beach sand. The review addresses strategies to assess beach sand quality, monitoring approaches, sand remediation, and the proposed way forward for beach sand monitoring programs. In the proposed way forward, recommendations are provided for acceptable levels of fungi given their distribution in the environment. Additional recommendations include evaluating FIB distributions at beaches globally to assess acceptable ranges of FIB levels, similar to those proposed for fungi.
    MeSH term(s) Sand ; Public Health ; Bacteria ; Water ; Fungi ; Bathing Beaches ; Water Microbiology ; Environmental Monitoring ; Feces/microbiology
    Chemical Substances Sand ; Water (059QF0KO0R)
    Language English
    Publishing date 2023-05-03
    Publishing country Switzerland
    Document type Journal Article ; Review ; Research Support, Non-U.S. Gov't
    ZDB-ID 2175195-X
    ISSN 1660-4601 ; 1661-7827
    ISSN (online) 1660-4601
    ISSN 1661-7827
    DOI 10.3390/ijerph20095710
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Climate Change Impacts on Microbiota in Beach Sand and Water: Looking Ahead.

    Brandão, João / Weiskerger, Chelsea / Valério, Elisabete / Pitkänen, Tarja / Meriläinen, Päivi / Avolio, Lindsay / Heaney, Christopher D / Sadowsky, Michael J

    International journal of environmental research and public health

    2022  Volume 19, Issue 3

    Abstract: Beach sand and water have both shown relevance for human health and their microbiology have been the subjects of study for decades. Recently, the World Health Organization recommended that recreational beach sands be added to the matrices monitored for ... ...

    Abstract Beach sand and water have both shown relevance for human health and their microbiology have been the subjects of study for decades. Recently, the World Health Organization recommended that recreational beach sands be added to the matrices monitored for enterococci and Fungi. Global climate change is affecting beach microbial contamination, via changes to conditions like water temperature, sea level, precipitation, and waves. In addition, the world is changing, and humans travel and relocate, often carrying endemic allochthonous microbiota. Coastal areas are amongst the most frequent relocation choices, especially in regions where desertification is taking place. A warmer future will likely require looking beyond the use of traditional water quality indicators to protect human health, in order to guarantee that waterways are safe to use for bathing and recreation. Finally, since sand is a complex matrix, an alternative set of microbial standards is necessary to guarantee that the health of beach users is protected from both sand and water contaminants. We need to plan for the future safer use of beaches by adapting regulations to a climate-changing world.
    MeSH term(s) Bathing Beaches ; Climate Change ; Environmental Monitoring ; Feces/microbiology ; Humans ; Microbiota ; Sand ; Water Microbiology
    Chemical Substances Sand
    Language English
    Publishing date 2022-01-27
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2175195-X
    ISSN 1660-4601 ; 1661-7827
    ISSN (online) 1660-4601
    ISSN 1661-7827
    DOI 10.3390/ijerph19031444
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Isolation and Characterization of Cylindrospermopsis raciborskii Strains from Finished Drinking Water

    Carina Menezes / Elisabete Valério / Maria João Botelho / Elsa Dias

    Toxins, Vol 12, Iss 1, p

    2020  Volume 40

    Abstract: In the summer of 2015, an intense cyanobacterial bloom producing geosmin/2-methylisoborneol (MIB) occurred in the Roxo freshwater reservoir in Alentejo, Portugal. The drinking water supplied from the Roxo water treatment plant (WTP) exhibited an ... ...

    Abstract In the summer of 2015, an intense cyanobacterial bloom producing geosmin/2-methylisoborneol (MIB) occurred in the Roxo freshwater reservoir in Alentejo, Portugal. The drinking water supplied from the Roxo water treatment plant (WTP) exhibited an unpleasant odor/taste and a significant cyanobacteria density was detected in the finished water at the exit of the WTP. Cyanobacteria were not evaluated downstream of the WTP, namely, at the city reservoir. The aim of this work was to isolate and characterize viable cyanobacteria present in finished water (exit of the WTP and city reservoir) that withstand conventional water treatment. Treated water samples collected at both sites were inoculated in Z8 culture medium to provide the conditions for putative cyanobacterial growth. After 30 days, filamentous cyanobacteria were observed in cultures inoculated with samples from the exit point of the WTP. Viable trichomes were isolated and identified as Cylindrospermopsis raciborskii by morphometric and molecular analysis. None of the isolates were cylindrospermopsin/microcystin producers, as confirmed by ELISA and amplification of corresponding genes ( PS / PKS and mcyA-cd / mcyAB / mcyB ). ELISA results were positive for saxitoxin, but saxitoxin and derivatives were not detected by liquid chromatography with fluorescence detection (LC-FLD), nor were their related genes ( sxtA / sxtA4 / sxtB / sxtM / sxtPer / sxtI ). To our knowledge, this is the first report on the establishment of cultures of C. raciborskii that resisted water treatment processes.
    Keywords cylindrospermopsis raciborskii ; finished water ; saxitoxin ; culture collection ; Medicine ; R
    Subject code 500
    Language English
    Publishing date 2020-01-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  8. Article ; Online: Strategies for Monitoring Microbial Life in Beach Sand for Protection of Public Health

    João Brandão / Elisabete Valério / Chelsea Weiskerger / Cristina Veríssimo / Konstantina Sarioglou / Monika Novak Babič / Helena M. Solo-Gabriele / Raquel Sabino / Maria Teresa Rebelo

    International Journal of Environmental Research and Public Health, Vol 20, Iss 5710, p

    2023  Volume 5710

    Abstract: The 2021 revised guidelines of the World Health Organization recommend monitoring the quality of sand in addition to water at recreational beaches. This review provides background information about the types of beaches, the characteristics of sand, and ... ...

    Abstract The 2021 revised guidelines of the World Health Organization recommend monitoring the quality of sand in addition to water at recreational beaches. This review provides background information about the types of beaches, the characteristics of sand, and the microbiological parameters that should be measured. Analytical approaches are described for quantifying fungi and fecal indicator bacteria from beach sand. The review addresses strategies to assess beach sand quality, monitoring approaches, sand remediation, and the proposed way forward for beach sand monitoring programs. In the proposed way forward, recommendations are provided for acceptable levels of fungi given their distribution in the environment. Additional recommendations include evaluating FIB distributions at beaches globally to assess acceptable ranges of FIB levels, similar to those proposed for fungi.
    Keywords sand ; recreational water ; fungi ; FIB ; MST ; monitoring program ; Medicine ; R
    Subject code 333
    Language English
    Publishing date 2023-05-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  9. Article ; Online: New Insights in

    Valério, Elisabete / Barreiros, Sara / Rodrigues, Sara / Turkina, Maria V / Vasconcelos, Vitor M / Campos, Alexandre

    Toxins

    2020  Volume 12, Issue 10

    Abstract: Microcystins (MCs) are hepatotoxins produced by some cyanobacteria. They are cyclic peptides that inhibit the serine/threonine protein phosphatases (PPs) PP1 and PP2A, especially PP2A. The inhibition of PP2A triggers a series of molecular events, which ... ...

    Abstract Microcystins (MCs) are hepatotoxins produced by some cyanobacteria. They are cyclic peptides that inhibit the serine/threonine protein phosphatases (PPs) PP1 and PP2A, especially PP2A. The inhibition of PP2A triggers a series of molecular events, which are responsible for most MC cytotoxic and genotoxic effects on animal cells. It is also known that MCs induce oxidative stress in cells due to the production of reactive oxygen species (ROS). However, a complete characterization of the toxic effects of MCs is still not accomplished. This study aimed to clarify additional molecular mechanisms involved in MC-LR toxicity, using
    MeSH term(s) DNA Damage ; DNA Replication/drug effects ; Dose-Response Relationship, Drug ; Gene Expression Regulation, Fungal/drug effects ; Marine Toxins/toxicity ; Microbial Viability/drug effects ; Microcystins/toxicity ; Oxidative Stress/drug effects ; Proteome/drug effects ; Proteomics ; Saccharomyces cerevisiae/drug effects ; Saccharomyces cerevisiae/genetics ; Saccharomyces cerevisiae/metabolism ; Saccharomyces cerevisiae Proteins/genetics ; Saccharomyces cerevisiae Proteins/metabolism
    Chemical Substances Marine Toxins ; Microcystins ; Proteome ; Saccharomyces cerevisiae Proteins ; cyanoginosin LR (EQ8332842Y)
    Language English
    Publishing date 2020-10-21
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2518395-3
    ISSN 2072-6651 ; 2072-6651
    ISSN (online) 2072-6651
    ISSN 2072-6651
    DOI 10.3390/toxins12100667
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Climate Change Impacts on Microbiota in Beach Sand and Water

    João Brandão / Chelsea Weiskerger / Elisabete Valério / Tarja Pitkänen / Päivi Meriläinen / Lindsay Avolio / Christopher D. Heaney / Michael J. Sadowsky

    International Journal of Environmental Research and Public Health, Vol 19, Iss 1444, p

    Looking Ahead

    2022  Volume 1444

    Abstract: Beach sand and water have both shown relevance for human health and their microbiology have been the subjects of study for decades. Recently, the World Health Organization recommended that recreational beach sands be added to the matrices monitored for ... ...

    Abstract Beach sand and water have both shown relevance for human health and their microbiology have been the subjects of study for decades. Recently, the World Health Organization recommended that recreational beach sands be added to the matrices monitored for enterococci and Fungi. Global climate change is affecting beach microbial contamination, via changes to conditions like water temperature, sea level, precipitation, and waves. In addition, the world is changing, and humans travel and relocate, often carrying endemic allochthonous microbiota. Coastal areas are amongst the most frequent relocation choices, especially in regions where desertification is taking place. A warmer future will likely require looking beyond the use of traditional water quality indicators to protect human health, in order to guarantee that waterways are safe to use for bathing and recreation. Finally, since sand is a complex matrix, an alternative set of microbial standards is necessary to guarantee that the health of beach users is protected from both sand and water contaminants. We need to plan for the future safer use of beaches by adapting regulations to a climate-changing world.
    Keywords climate change ; global warming ; beach sand ; FIB ; sand ; recreational water ; Medicine ; R
    Subject code 333
    Language English
    Publishing date 2022-01-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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