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Article ; Online: The use of X-ray as an effective adjunct to the national hip screening policy for developmental dysplasia of the hip: experience at an Irish paediatric unit.

Coleman, John / Javed, Mehwish / Khurshid, Sundas / Mahmood, Lubna

2023 Volume 192, Issue 5, Page(s) 2435–2436

MeSH term(s)	Child ; Humans ; Infant ; X-Rays ; Developmental Dysplasia of the Hip ; Hip Dislocation, Congenital/diagnostic imaging ; Mass Screening ; Radiography ; Ultrasonography
Language	English
Publishing date	2023-02-02
Publishing country	Ireland
Document type	Letter
ZDB-ID	390895-1
ISSN	1863-4362 ; 0021-1265
ISSN (online)	1863-4362
ISSN	0021-1265
DOI	10.1007/s11845-023-03295-9
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Article ; Online: Functional characterisation of

Alam, Osama / Khan, Latif Ullah / Khan, Adeel / Salmen, Saleh H / Ansari, Mohammad Javed / Mehwish, Fizza / Ahmad, Mushtaq / Zaman, Qamar U / Wang, Hua-Feng

Functional plant biology : FPB

2024 Volume 51

Abstract: DNA binding proteins with one finger (Dof ) transcription factors are essential for seed development and defence against various biotic and abiotic stresses in plants. Genomic analysis of Dof has not been determined yet in pitaya (Selenicereus undatus ). ...

Abstract	DNA binding proteins with one finger (Dof ) transcription factors are essential for seed development and defence against various biotic and abiotic stresses in plants. Genomic analysis of Dof has not been determined yet in pitaya (Selenicereus undatus ). In this study, we have identified 26 Dof gene family members, renamed as HuDof-1 to HuDof-26 , and clustered them into seven subfamilies based on conserved motifs, domains, and phylogenetic analysis. The gene pairs of Dof family members were duplicated by segmental duplications that faced purifying selection, as indicated by the K a /K s ratio values. Promoter regions of HuDof genes contain many cis -acting elements related to phytohormones including abscisic acid, jasmonic acid, gibberellin, temperature, and light. We exposed pitaya plants to different environmental stresses and examined melatonin's influence on Dof gene expression levels. Signifcant expression of HuDof -2 and HuDof -6 were observed in different developmental stages of flower buds, flowers, pericarp, and pulp. Pitaya plants were subjected to abiotic stresses, and transcriptome analysis was carried out to identify the role of Dof gene family members. RNA-sequencing data and reverse transcription quantitative PCR-based expression analysis revealed three putative candidate genes (HuDof -1, HuDof -2, and HuDof -8), which might have diverse roles against the abiotic stresses. Our study provides a theoretical foundation for functional analysis through traditional and modern biotechnological tools for pitaya trait improvement.
MeSH term(s)	Melatonin ; Phylogeny ; Stress, Physiological/genetics ; Transcription Factors/genetics ; Transcription Factors/metabolism ; Cactaceae
Chemical Substances	Melatonin (JL5DK93RCL) ; Transcription Factors
Language	English
Publishing date	2024-04-03
Publishing country	Australia
Document type	Journal Article
ZDB-ID	2071582-1
ISSN	1445-4416 ; 1445-4408
ISSN (online)	1445-4416
ISSN	1445-4408
DOI	10.1071/FP23269
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Article: Prevalence of irritable bowel syndrome and functional dyspepsia and their relationship with generalized anxiety disorder among medical students in Karachi.

Javed, Mahnoor / Yadav, Tularam / Shakil, Jibran / Haider Zaidi, Syed Tafazzul / Zehra, Mehwish

JPMA. The Journal of the Pakistan Medical Association

2023 Volume 72, Issue 10, Page(s) 2132–2136

Abstract: Objective: To assess the prevalence of inflammatory bowel syndrome and functional dyspepsia in medical students and their relationship with generalised anxiety disorder and other relevant factors.: Methods: The descriptive cross-sectional study was ... ...

Abstract	Objective: To assess the prevalence of inflammatory bowel syndrome and functional dyspepsia in medical students and their relationship with generalised anxiety disorder and other relevant factors. Methods: The descriptive cross-sectional study was conducted in Karachi from August to November 2020 after approval from the ethics review board of Jinnah Sindh Medical University, and comprised students of either gender aged at least 18 years from different medical colleges in the city. Data was collected using validated tools Rome III criteria for inflammatory bowel syndrome and functional dyspepsia and Generalised Anxiety Disorder-7. Data was analysed using SPSS 26. Results: Of the 305 subjects, 185(60.70%) were male and 120(39.30%) were female. The overall mean age was 21.43±2.13 years. The prevalence of inflammatory bowel syndrome was 17(5.57%), while that of functional dyspepsia was 2(0.66%). Further, 122(40%) students had minimal anxiety, 112(36.72%) mild, 43(14.10%) moderate and 28(9.18%) had severe anxiety. There was no significant relationship of either inflammatory bowel syndrome or functional dyspepsia diagnosis with generalised anxiety disorder (p>0.05), but a significant relationship existed between inflammatory bowel syndrome diagnosis and generalised anxiety disorder score (p=0.041). Significant relationships were found between inflammatory bowel syndrome diagnosis and use of non-steroidal anti-inflammatory drugs. The relationship of generalised anxiety disorder diagnosis with gender and probiotic use was significant (p<0.05), and so was the relationship of generalised anxiety disorder score with gender and level of physical activity (p<0.05). Conclusions: Medical students with a higher generalised anxiety disorder score and those who used non-steroidal anti-inflammatory drugs frequently had a higher likelihood of developing inflammatory bowel syndrome.
MeSH term(s)	Humans ; Male ; Female ; Adolescent ; Adult ; Young Adult ; Dyspepsia/epidemiology ; Irritable Bowel Syndrome/epidemiology ; Irritable Bowel Syndrome/diagnosis ; Students, Medical ; Prevalence ; Cross-Sectional Studies ; Surveys and Questionnaires ; Anxiety Disorders/epidemiology ; Anti-Inflammatory Agents
Chemical Substances	Anti-Inflammatory Agents
Language	English
Publishing date	2023-01-24
Publishing country	Pakistan
Document type	Journal Article
ZDB-ID	603873-6
ISSN	0030-9982
ISSN	0030-9982
DOI	10.47391/JPMA.4927
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Article: Investigating the role of carbohydrate-binding module 34 in cyclomaltodextrinase from

Aroob, Iqra / Javed, Maryam / Ahmad, Nasir / Aslam, Mehwish / Rashid, Naeem

3 Biotech

2021 Volume 12, Issue 1, Page(s) 25

Abstract: Carbohydrate-binding modules (CBMs) are noncatalytic regions found in several enzymes of glycoside hydrolase family 13 and are proposed to orient substrates to the catalytic site. In this study, a substantial information on the conserved aromatic ... ...

Abstract	Carbohydrate-binding modules (CBMs) are noncatalytic regions found in several enzymes of glycoside hydrolase family 13 and are proposed to orient substrates to the catalytic site. In this study, a substantial information on the conserved aromatic residues in CBM34 regions of characterized bacterial cyclolmaltodextrinases (CDases) has been presented. Molecular modeling of CDase from Supplementary information: The online version contains supplementary material available at 10.1007/s13205-021-03089-9.
Language	English
Publishing date	2021-12-23
Publishing country	Germany
Document type	Journal Article
ZDB-ID	2600522-0
ISSN	2190-5738 ; 2190-572X
ISSN (online)	2190-5738
ISSN	2190-572X
DOI	10.1007/s13205-021-03089-9
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Article ; Online: Chitosan based smart polymer composites: Fabrication and pH-Responsive behavior for bio-medical applications.

Ma, Xijun / Zhang, Jing / Javed, Mehwish / Wu, Jiayu / Hu, Yuzhu / Yin, Siyuan / Zhu, Yujie / Wu, Wentian / Liu, Fei

Environmental research

2023 Volume 221, Page(s) 115286

Abstract: This research aimed to synthesize Chitosan/PVA-blank and a series of Cs/PVA/Sepolite based pH-sensitive membranes using a solution casting process. The synthesized Cs/PVA-blank and Cs/PVA/Sep based membranes were investigated via SEM, FTIR, XRD, and TGA ... ...

Abstract	This research aimed to synthesize Chitosan/PVA-blank and a series of Cs/PVA/Sepolite based pH-sensitive membranes using a solution casting process. The synthesized Cs/PVA-blank and Cs/PVA/Sep based membranes were investigated via SEM, FTIR, XRD, and TGA techniques. The SEM results of Cs/PVA/Sep based membrane reveal that the hydrolytic stability and strength were improved in acidic and basic media owing to the incorporation of sepiolite content into chitosan. The characteristic band at 3741 cm
MeSH term(s)	Chitosan ; Stimuli Responsive Polymers ; Hydrogen-Ion Concentration ; Water
Chemical Substances	Chitosan (9012-76-4) ; magnesium trisilicate (C2E1CI501T) ; Stimuli Responsive Polymers ; Water (059QF0KO0R)
Language	English
Publishing date	2023-01-13
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	205699-9
ISSN	1096-0953 ; 0013-9351
ISSN (online)	1096-0953
ISSN	0013-9351
DOI	10.1016/j.envres.2023.115286
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Article ; Online: Bacterial-Mediated Salinity Stress Tolerance in Maize (

Ali, Baber / Hafeez, Aqsa / Afridi, Muhammad Siddique / Javed, Muhammad Ammar / Sumaira / Suleman, Faiza / Nadeem, Mehwish / Ali, Shehzad / Alwahibi, Mona S / Elshikh, Mohamed S / Marc, Romina Alina / Ercisli, Sezai / Darwish, Doaa Bahaa Eldin

ACS omega

2023 Volume 8, Issue 23, Page(s) 20471–20487

Abstract: Sustainable agriculture is threatened by salinity stress because of the low yield quality and low crop production. Rhizobacteria that promote plant growth modify physiological and molecular pathways to support plant development and reduce abiotic ... ...

Abstract	Sustainable agriculture is threatened by salinity stress because of the low yield quality and low crop production. Rhizobacteria that promote plant growth modify physiological and molecular pathways to support plant development and reduce abiotic stresses. The recent study aimed to assess the tolerance capacity and impacts of
Language	English
Publishing date	2023-05-26
Publishing country	United States
Document type	Journal Article
ISSN	2470-1343
ISSN (online)	2470-1343
DOI	10.1021/acsomega.3c00723
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Article: Investigating the role of carbohydrate-binding module 34 in cyclomaltodextrinase from Geobacillus thermopakistaniensis: structural and functional analyses

Aroob, Iqra / Javed, Maryam / Ahmad, Nasir / Aslam, Mehwish / Rashid, Naeem

3 Biotech. 2022 Jan., v. 12, no. 1

2022

Abstract	Carbohydrate-binding modules (CBMs) are noncatalytic regions found in several enzymes of glycoside hydrolase family 13 and are proposed to orient substrates to the catalytic site. In this study, a substantial information on the conserved aromatic residues in CBM34 regions of characterized bacterial cyclolmaltodextrinases (CDases) has been presented. Molecular modeling of CDase from Geobacillus thermopakistaniensis (CDaseGₜ) revealed a change in the active site geometry due to CBM34 truncation. The binding energies of full-length (CDaseGₜ) and CBM34 truncated (CDaseGₜ-ΔN) models showed opposite trends. The least preferred substrate molecule by the full-length model was the most preferred by the CBM34 truncated one. These exciting in silico findings were experimentally verified by recombinant production and characterization of the full-length and the CBM34 truncated proteins. Both the enzymes showed similar optimum pH and temperature. However, substrate specificity was in the reverse order. These experimental verifications matched the homology modeling and docking predictions.
Keywords	Geobacillus ; active sites ; carbohydrate binding ; computer simulation ; geometry ; glycosides ; hydrolases ; pH ; substrate specificity ; temperature
Language	English
Dates of publication	2022-01
Size	p. 25.
Publishing place	Springer International Publishing
Document type	Article
ZDB-ID	2600522-0
ISSN	2190-5738 ; 2190-572X
ISSN (online)	2190-5738
ISSN	2190-572X
DOI	10.1007/s13205-021-03089-9
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Article ; Online: AI- modelling of molecular identification and feminization of wolbachia infected Aedes aegypti.

Iftikhar, Mehwish / Iftikhar, Sahrish / Sohail, Ayesha / Javed, Sana

Progress in biophysics and molecular biology

2019 Volume 150, Page(s) 104–111

Abstract: Background: The genetic control strategies of vector borne diseases includes the replacement of a vector population by "disease-refractory" mosquitoes and the release of mosquitoes with a gene to control the vector's reproduction rates. Wolbachia are ... ...

Abstract	Background: The genetic control strategies of vector borne diseases includes the replacement of a vector population by "disease-refractory" mosquitoes and the release of mosquitoes with a gene to control the vector's reproduction rates. Wolbachia are common intracellular bacteria that are found in arthropods and nematodes. Wolbachia infected male mosquitos have been used in different experimental trials around the world to suppress the target population of Aedes aegypti and this genetic control strategy has proved to be a promising alternative to other treatment strategies. Due to certain limitations, the successful application of this strategy is still awaited. Methods: Mathematical frame work for Wolbachia induced genetic control strategy has been developed in this article. With the aid of Artificial Intelligence (AI) tools, accurate parametric values are depicted. For the first time, the model is well synchronized with the experimental findings. The model is comprised of the generalized varying coefficient and multiple mating rates between infected and uninfected compartments of Aedes aegypti dengue to forecast the disease control. Results: Two mathematical models are developed in this article to demonstrate different mating rates of the genetic control strategy. The important parameters and time varying coefficients are well demonstrated with the aid of numerical computations. The resulting thresholds and forecasting may prove to be a useful tool for future experimental studies. Conclusions: From our analysis, we have concluded that the genetic control strategy is a promising technique and the role of Wolbachia infected male mosquitos, in genetic control strategies, can be better interpreted in an inexpensive manner with the aid of a theoretical model.
MeSH term(s)	Aedes/genetics ; Aedes/microbiology ; Animals ; Artificial Intelligence ; Computer Simulation ; Feminization/genetics ; Humans ; Infections ; Male ; Models, Biological ; Models, Theoretical ; Mosquito Vectors/microbiology ; Reproduction/genetics ; Systems Analysis ; Wolbachia/genetics
Language	English
Publishing date	2019-07-11
Publishing country	England
Document type	Journal Article
ZDB-ID	209302-9
ISSN	1873-1732 ; 0079-6107
ISSN (online)	1873-1732
ISSN	0079-6107
DOI	10.1016/j.pbiomolbio.2019.07.001
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Article: Comparison of effectiveness of muscle energy technique with Mulligan mobilization in patients with non-specific neck pain.

Manzoor, Anam / Anwar, Naveed / Khalid, Kehkshan / Haider, Rizwan / Saghir, Mehwish / Javed, Muhammad Asif

JPMA. The Journal of the Pakistan Medical Association

2021 Volume 71, Issue 6, Page(s) 1532–1524

Abstract: Objective: To compare the efficacy of muscle energy technique with Mulligan mobilisation in patients having non-specific neck pain.: Methods: The quasi-experimental study was conducted at the Physiotherapy Department of Mayo Hospital, Lahore, ... ...

Abstract	Objective: To compare the efficacy of muscle energy technique with Mulligan mobilisation in patients having non-specific neck pain. Methods: The quasi-experimental study was conducted at the Physiotherapy Department of Mayo Hospital, Lahore, Pakistan, from March to September 2017, and comprised patients of either gender having non-specific neck pain who ere divided into two groups. In group 1 patients, muscle energy technique was used, while group 2 patients had Mulligan mobilisation. Pain intensity, functional status of neck and cervical range of motion were measured before and after treatment. Data was analysed using SPSS 21. Results: Of the 56 patients, 28(50%) were in each of the two groups. The overall mean age was 36.89±9.28 years. Pain intensity, functional status and neck extension range of motion improved significantly more in group 2 (p<0.05). Conclusions: Mulligan mobilisation was found to be a better option in managing patients with non-specific neck pain.
MeSH term(s)	Adult ; Chest Pain ; Humans ; Middle Aged ; Muscles ; Neck Pain/therapy ; Pakistan ; Range of Motion, Articular
Language	English
Publishing date	2021-06-10
Publishing country	Pakistan
Document type	Journal Article
ZDB-ID	603873-6
ISSN	0030-9982
ISSN	0030-9982
DOI	10.47391/JPMA.981
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Article ; Online: Human Coronavirus Spike Protein Based Multi-Epitope Vaccine against COVID-19 and Potential Future Zoonotic Coronaviruses by Using Immunoinformatic Approaches

Zulqarnain Baloch / Aqsa Ikram / Saba Shamim / Ayesha Obaid / Faryal Mehwish Awan / Anam Naz / Bisma Rauff / Khadija Gilani / Javed Anver Qureshi

Vaccines, Vol 10, Iss 1150, p

2022 Volume 1150

Abstract: Zoonotic coronaviruses (CoV) have emerged twice and have caused severe respiratory diseases in humans. Due to the frequent outbreaks of different human coronaviruses (HCoVs), the development of a pan-HCoV vaccine is of great importance. Various conserved ...

Abstract	Zoonotic coronaviruses (CoV) have emerged twice and have caused severe respiratory diseases in humans. Due to the frequent outbreaks of different human coronaviruses (HCoVs), the development of a pan-HCoV vaccine is of great importance. Various conserved epitopes shared by HCoVs are reported to induce cross-reactive T-cell responses. Therefore, this study aimed to design a multi-epitope vaccine, targeting the HCoV spike protein. Genetic analysis revealed that the spike region is highly conserved among SARS-CoV-2, bat SL-CoV, and SARS-CoV. By employing the immunoinformatic approach, we prioritized 20 MHC I and 10 MHCII conserved epitopes to design a multi-epitope vaccine. This vaccine candidate is anticipated to strongly elicit both humoral and cell-mediated immune responses. These results warrant further development of this vaccine into real-world application.
Keywords	SARS-CoV-2 ; bat SL-CoV and SARS-CoV ; immunoinformatics ; vaccine ; Medicine ; R
Subject code	570
Language	English
Publishing date	2022-07-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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