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  1. Article: Peanut contamination in food products: a real danger for allergic people?

    Krejner-Bienias, Alicja / Grzela, Katarzyna / Kulus, Marek / Grzela, Tomasz

    Postepy dermatologii i alergologii

    2023  Volume 40, Issue 5, Page(s) 625–629

    Abstract: Introduction: Allergy to peanut affects approximately 2% of children and in most cases persists throughout adult life. Seventeen peanut allergens have been identified so far and registered as "Ara h" molecules. Two of them, Ara h 1 and Ara h 3, are the ... ...

    Abstract Introduction: Allergy to peanut affects approximately 2% of children and in most cases persists throughout adult life. Seventeen peanut allergens have been identified so far and registered as "Ara h" molecules. Two of them, Ara h 1 and Ara h 3, are the most abundant proteins in the peanut extract. Since strict avoidance of peanut-containing food is the easiest way to prevent severe allergic reactions, manufacturers must label such products. However, consumers can still inadvertently be exposed to peanut allergens when foods become contaminated from processing lines shared with peanut products.
    Aim: To investigate whether food products with the label "may contain traces of peanuts", available on the Polish market, are actually contaminated with Ara h 1 and Ara h 3.
    Material and methods: Thirty food products with the label "may contain traces of peanuts", were purchased in Polish stores. Samples of the foods were analyzed by using Ara h 1/Ara h 3 ELISA kits.
    Results: Nearly one third of tested food products contained clinically relevant amounts of Ara h 1 and Ara h 3. The doses of both peanut allergens, when adjusted to the serving size of tested products, exceeded several times the eliciting dose 05 (the amount of the allergen, which is predicted to provoke a reaction in 5% of at-the-risk allergic population).
    Conclusions: Consumption of foods labelled as "may contain traces of peanuts" poses a significant risk for people allergic to peanuts. Physicians should advise their patients with peanut allergy to strictly avoid such products.
    Language English
    Publishing date 2023-11-09
    Publishing country Poland
    Document type Journal Article
    ZDB-ID 2596142-1
    ISSN 1642-395X
    ISSN 1642-395X
    DOI 10.5114/ada.2023.132219
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: DPP4 Inhibitors and COVID-19-Holy Grail or Another Dead End?

    Krejner-Bienias, Alicja / Grzela, Katarzyna / Grzela, Tomasz

    Archivum immunologiae et therapiae experimentalis

    2021  Volume 69, Issue 1, Page(s) 1

    Abstract: A novel coronavirus disease, COVID-19, has emerged as a global public health issue. Clinical course of disease significantly correlates with the occurrence of some comorbidities, among them type 2 diabetes. According to recent structural studies the ... ...

    Abstract A novel coronavirus disease, COVID-19, has emerged as a global public health issue. Clinical course of disease significantly correlates with the occurrence of some comorbidities, among them type 2 diabetes. According to recent structural studies the dipeptidyl peptidase 4, a key molecule in the pathophysiology of diabetes, may influence the course of COVID-19. Since DPP4 inhibitors, gliptins, are widely used in diabetes patients, the exact role of DPP4 modulation in SARS-CoV-2 infection, at least in that group, urgently needs to be clarified. In this short review, we discuss this issue with more detail.
    MeSH term(s) Antiviral Agents/pharmacology ; Antiviral Agents/therapeutic use ; COVID-19/complications ; COVID-19/drug therapy ; COVID-19/enzymology ; COVID-19/metabolism ; Diabetes Mellitus, Type 2/complications ; Dipeptidyl Peptidase 4/metabolism ; Dipeptidyl-Peptidase IV Inhibitors/pharmacology ; Dipeptidyl-Peptidase IV Inhibitors/therapeutic use ; Humans ; SARS-CoV-2/drug effects
    Chemical Substances Antiviral Agents ; Dipeptidyl-Peptidase IV Inhibitors ; DPP4 protein, human (EC 3.4.14.5) ; Dipeptidyl Peptidase 4 (EC 3.4.14.5)
    Language English
    Publishing date 2021-02-02
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 282209-x
    ISSN 1661-4917 ; 0004-069X
    ISSN (online) 1661-4917
    ISSN 0004-069X
    DOI 10.1007/s00005-020-00602-5
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Do novel drugs for diabetes help in COVID-19? Another brick in the wall?

    Krejner-Bienias, Alicja / Grzela, Katarzyna / Grzela, Tomasz

    Journal of diabetes

    2020  Volume 12, Issue 9, Page(s) 703–704

    MeSH term(s) Betacoronavirus/drug effects ; COVID-19 ; Coronavirus Infections/drug therapy ; Coronavirus Infections/epidemiology ; Coronavirus Infections/virology ; Diabetes Mellitus/drug therapy ; Diabetes Mellitus/epidemiology ; Diabetes Mellitus/virology ; Dipeptidyl Peptidase 4/metabolism ; Humans ; Hypoglycemic Agents/therapeutic use ; Pandemics ; Pneumonia, Viral/drug therapy ; Pneumonia, Viral/epidemiology ; Pneumonia, Viral/virology ; SARS-CoV-2
    Chemical Substances Hypoglycemic Agents ; DPP4 protein, human (EC 3.4.14.5) ; Dipeptidyl Peptidase 4 (EC 3.4.14.5)
    Keywords covid19
    Language English
    Publishing date 2020-05-22
    Publishing country Australia
    Document type Letter
    ZDB-ID 2503337-2
    ISSN 1753-0407 ; 1753-0393
    ISSN (online) 1753-0407
    ISSN 1753-0393
    DOI 10.1111/1753-0407.13050
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Do novel drugs for diabetes help in COVID ‐19? Another brick in the wall?

    Krejner‐Bienias, Alicja / Grzela, Katarzyna / Grzela, Tomasz

    Journal of Diabetes

    2020  Volume 12, Issue 9, Page(s) 703–704

    Keywords Endocrinology, Diabetes and Metabolism ; covid19
    Language English
    Publisher Wiley
    Publishing country us
    Document type Article ; Online
    ZDB-ID 2503337-2
    ISSN 1753-0393
    ISSN 1753-0393
    DOI 10.1111/1753-0407.13050
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article: Exhaled breath condensates from healthy children induce cell death of

    Krejner-Bienias, Alicja / Grzela, Katarzyna / Zagórska, Wioletta / Chojnowska, Magdalena / Grzela, Tomasz

    Postepy dermatologii i alergologii

    2020  Volume 38, Issue 2, Page(s) 85–90

    Abstract: Introduction: Exhaled breath condensate (EBC) is a liquefied air, containing a mixture of non-volatile compounds, reflecting pathophysiological status of the bronchopulmonary system. Therefore, EBC analysis may be useful in diagnostics and monitoring of ...

    Abstract Introduction: Exhaled breath condensate (EBC) is a liquefied air, containing a mixture of non-volatile compounds, reflecting pathophysiological status of the bronchopulmonary system. Therefore, EBC analysis may be useful in diagnostics and monitoring of various respiratory diseases. In previous studies it was found that EBC from asthmatic children contained several regulators of angiogenesis.
    Aim: To assess the expression of selected apoptosis-related proteins in human and murine cells exposed to EBC from healthy children.
    Material and methods: EBCs from healthy children were added to cultures of murine endothelial cells (C166) or human lung fibroblasts (HLF) to induce their apoptosis. For proteome analysis the apoptosis pathway-specific protein microarrays were used.
    Results: The homogenates from EBC-treated C166 cells contained low amounts of Hsp27, which correlated with their fast death. Contrary to C166, the lysates from EBC-treated fibroblasts displayed increased amounts of Hsp27, which correlated with delayed HLF response to the induction of apoptosis. Except for increased caspase-3 in EBC-treated HLF, none of the other apoptosis regulators revealed any significant changes in that analysis.
    Conclusions: The screening of apoptosis pathways with microarray technology allowed identification of two molecules, Hsp27 and caspase-3, involved in cellular response to EBC. However, the factor responsible for induction of the cytotoxic effect of EBC from healthy children still remains unknown.
    Language English
    Publishing date 2020-11-11
    Publishing country Poland
    Document type Journal Article
    ZDB-ID 2596142-1
    ISSN 1642-395X
    ISSN 1642-395X
    DOI 10.5114/ada.2019.87087
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Influence of exhaled breath condensates from children with asthma on endothelial cells cultured

    Krejner-Bienias, Alicja / Grzela, Katarzyna / Zagórska, Wioletta / Chojnowska, Magdalena / Świrska, Maja / Grzela, Tomasz

    Postepy dermatologii i alergologii

    2019  Volume 37, Issue 6, Page(s) 368–374

    Abstract: Introduction: Asthma-associated remodelling involves subepithelial fibrosis and increased vascularization of the bronchial wall. The latter may be associated with excessive production of several angiogenesis regulators which may be found in exhaled ... ...

    Abstract Introduction: Asthma-associated remodelling involves subepithelial fibrosis and increased vascularization of the bronchial wall. The latter may be associated with excessive production of several angiogenesis regulators which may be found in exhaled breath condensates (EBCs) collected from children with asthma.
    Aim: To assess the influence of EBC samples of asthmatic children and healthy controls on
    Material and methods: Breath condensates collected from children with mild asthma (
    Results: The EBCs from patients with asthma revealed a weak inhibitory influence on human and murine endothelial cells. Surprisingly, EBCs from healthy children led to cell death, mainly by the induction of apoptosis. There were no statistically significant differences in the cytokine profile between EBC samples from children with asthma and healthy controls.
    Conclusions: Our preliminary report shows for the first time that the incubation of EBCs from healthy controls induced apoptosis in endothelial cells. The detailed mechanism responsible for this action remains unknown and requires further research.
    Language English
    Publishing date 2019-07-29
    Publishing country Poland
    Document type Journal Article
    ZDB-ID 2596142-1
    ISSN 1642-395X
    ISSN 1642-395X
    DOI 10.5114/ada.2019.86181
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: Is the composition of exhaled breath condensate a key to explain the course of COVID-19 in children?

    Krejner-Bienias, Alicja / Grzela, Katarzyna / Krenke, Rafal / Gorska, Katarzyna / Nejman-Gryz, Patrycja / Stadnik, Dorota / Kobylska, Ewa / Grzela, Tomasz

    Postepy dermatologii i alergologii

    2020  Volume 38, Issue 6, Page(s) 1001–1005

    Abstract: Introduction: The relative resistance of children to severe course of the novel coronavirus infection remains unclear. We hypothesized that there might be a link between this phenomenon and observation from our previous studies concerning an inhibitory ... ...

    Abstract Introduction: The relative resistance of children to severe course of the novel coronavirus infection remains unclear. We hypothesized that there might be a link between this phenomenon and observation from our previous studies concerning an inhibitory or cytotoxic effect of exhaled breath condensate (EBC) on endothelial cell cultures in children.
    Aim: Since we could not find any data on the similar effect caused by EBC in adults, the aim of our study was to evaluate and compare the biological activity of EBC in adults and children in an experimental
    Material and methods: The influence of EBC samples on metabolic activity of endothelial cell line C-166 was assessed using colorimetric tetrazolium salt reduction assay (MTT assay). Selected EBC samples were fractionated using size exclusion chromatography and subjected to mass spectrometry analysis.
    Results: Exhaled breath condensates in healthy children, but not in adults, revealed a cytotoxic effect on
    Conclusions: Breath condensates of healthy children contain the factor which reveals the inhibitory/cytotoxic effect on endothelial cell cultures. Although the physiological role of this agent remains unclear, its identification may potentially be useful in ongoing research on SARS-CoV-2/COVID-19.
    Language English
    Publishing date 2020-11-12
    Publishing country Poland
    Document type Journal Article
    ZDB-ID 2596142-1
    ISSN 1642-395X
    ISSN 1642-395X
    DOI 10.5114/ada.2020.97395
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Exhaled breath condensates from healthy children induce cell death of in vitro cultured cells by activation of apoptosis

    Alicja Krejner-Bienias / Katarzyna Grzela / Wioletta Zagórska / Magdalena Chojnowska / Tomasz Grzela

    Advances in Dermatology and Allergology, Vol 38, Iss 1, Pp 85-

    2019  Volume 90

    Keywords apoptosis ; exhaled breath condensate ; protein microarray ; Dermatology ; RL1-803 ; Internal medicine ; RC31-1245
    Language English
    Publishing date 2019-11-01T00:00:00Z
    Publisher Termedia Publishing House
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  9. Article ; Online: Influence of exhaled breath condensates from children with asthma on endothelial cells cultured in vitro. Do we really know everything about our breath condensate?

    Alicja Krejner-Bienias / Katarzyna Grzela / Wioletta Zagórska / Magdalena Chojnowska / Maja Świrska / Tomasz Grzela

    Advances in Dermatology and Allergology, Vol 37, Iss 6, Pp 968-

    2019  Volume 674

    Keywords angiogenesis ; asthma ; endothelial cells ; breath condensate ; mtt ; Dermatology ; RL1-803 ; Internal medicine ; RC31-1245
    Language English
    Publishing date 2019-07-01T00:00:00Z
    Publisher Termedia Publishing House
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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