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  1. Book: Alternative splicing in cancer

    Venables, Julian P.

    2006  

    Author's details ed. Julian P. Venables
    Language English
    Size 309 S. : Ill., graph. Darst.
    Publisher Transworld Research Network
    Publishing place Trivandrum
    Publishing country India
    Document type Book
    HBZ-ID HT015414306
    ISBN 81-7895-235-1 ; 978-81-7895-235-2
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

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    2008 A 1077 order for loan Magazin

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  2. Article: SPF45/RBM17-dependent splicing and multidrug resistance to cancer chemotherapy.

    Fukumura, Kazuhiro / Venables, Julian P / Mayeda, Akila

    Molecular & cellular oncology

    2021  Volume 8, Issue 6, Page(s) 1996318

    Abstract: The early splicing complex A occupies at least eighty nucleotides of intron, in which U2AF covers the polypyrimidine tract. SPF45 (RBM17) functionally substitutes for U2AF on a subset of short introns. Since SPF45 expression confers resistance to various ...

    Abstract The early splicing complex A occupies at least eighty nucleotides of intron, in which U2AF covers the polypyrimidine tract. SPF45 (RBM17) functionally substitutes for U2AF on a subset of short introns. Since SPF45 expression confers resistance to various anticancer drugs, SPF45-dependent splicing may play a critical role in multidrug resistance.
    Language English
    Publishing date 2021-11-15
    Publishing country United States
    Document type Journal Article
    ISSN 2372-3556
    ISSN 2372-3556
    DOI 10.1080/23723556.2021.1996318
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Enrichment of alternatively spliced isoforms.

    Venables, Julian P

    Methods in molecular biology (Clifton, N.J.)

    2008  Volume 419, Page(s) 161–170

    Abstract: Most metazoan genes are alternatively spliced, and a large number of alternatively spliced isoforms are likely to be functionally significant and expressed at specific stages of pathogenesis or differentiation. Splicing changes usually only affect a ... ...

    Abstract Most metazoan genes are alternatively spliced, and a large number of alternatively spliced isoforms are likely to be functionally significant and expressed at specific stages of pathogenesis or differentiation. Splicing changes usually only affect a small portion of a gene, and these changes may cause significant mRNA degradation. After RT-PCR, minor variants can form heteroduplexes with the major variants. Affinity purification of these heteroduplexes using immobilized Thermus aquaticus single-stranded DNA-binding protein allows purification of alternative splice forms in a 1:1 ratio, which makes it easy to sequence the rare form. This chapter provides a detailed protocol of the technique I have developed to identify spliced isoforms called enrichment of alternatively spliced isoforms or EASI.
    MeSH term(s) Alternative Splicing ; Bacterial Proteins/metabolism ; Base Sequence ; DNA Primers/genetics ; DNA-Binding Proteins/metabolism ; Humans ; Male ; Nucleic Acid Heteroduplexes/genetics ; Nucleic Acid Heteroduplexes/isolation & purification ; Nucleic Acid Heteroduplexes/metabolism ; Polymerase Chain Reaction ; RNA, Messenger/genetics ; RNA, Messenger/isolation & purification ; RNA, Messenger/metabolism ; Recombinant Proteins/metabolism ; Testis/metabolism ; Thermus/metabolism
    Chemical Substances Bacterial Proteins ; DNA Primers ; DNA-Binding Proteins ; Nucleic Acid Heteroduplexes ; RNA, Messenger ; Recombinant Proteins
    Language English
    Publishing date 2008
    Publishing country United States
    Document type Journal Article
    ISSN 1064-3745
    ISSN 1064-3745
    DOI 10.1007/978-1-59745-033-1_11
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Downstream intronic splicing enhancers.

    Venables, Julian P

    FEBS letters

    2007  Volume 581, Issue 22, Page(s) 4127–4131

    Abstract: Alternative splicing leads to multiple proteins from individual genes and the most common deviation from the norm is precise exon omission. Mutations that cause this can be found deep in introns, especially downstream of the cassette exon. This review ... ...

    Abstract Alternative splicing leads to multiple proteins from individual genes and the most common deviation from the norm is precise exon omission. Mutations that cause this can be found deep in introns, especially downstream of the cassette exon. This review summarises what is known about these intronic splicing enhancers and their RNA-binding proteins that cause spliceosome assembly on the upstream exon.
    MeSH term(s) Alternative Splicing/genetics ; Animals ; Exons/genetics ; Humans ; Introns/genetics ; Neurons/metabolism ; Nuclear Proteins/metabolism ; RNA-Binding Proteins/metabolism
    Chemical Substances Nuclear Proteins ; RNA-Binding Proteins
    Language English
    Publishing date 2007-09-04
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 212746-5
    ISSN 1873-3468 ; 0014-5793
    ISSN (online) 1873-3468
    ISSN 0014-5793
    DOI 10.1016/j.febslet.2007.08.012
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Unbalanced alternative splicing and its significance in cancer.

    Venables, Julian P

    BioEssays : news and reviews in molecular, cellular and developmental biology

    2006  Volume 28, Issue 4, Page(s) 378–386

    Abstract: Alternative pre-mRNA splicing leads to distinct products of gene expression in development and disease. Antagonistic splice variants of genes involved in differentiation, apoptosis, invasion and metastasis often exist in a delicate equilibrium that is ... ...

    Abstract Alternative pre-mRNA splicing leads to distinct products of gene expression in development and disease. Antagonistic splice variants of genes involved in differentiation, apoptosis, invasion and metastasis often exist in a delicate equilibrium that is found to be perturbed in tumours. In several recent examples, splice variants that are overexpressed in cancer are expressed as hyper-oncogenic proteins, which often correlate with poor prognosis, thus suggesting improved diagnosis and follow up treatment. Global gene expression technologies are just beginning to decipher the interplay between alternatively spliced isoforms and protein-splicing factors that will lead to identification of the mutations in these trans-acting factors responsible for pathogenic alternative splicing in cancer.
    MeSH term(s) Alternative Splicing/genetics ; Animals ; Apoptosis ; Cell Differentiation ; Gene Expression Regulation, Neoplastic/genetics ; Humans ; Neoplasm Invasiveness/pathology ; Neoplasms/blood supply ; Neoplasms/genetics ; Neoplasms/pathology
    Language English
    Publishing date 2006-04
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 50140-2
    ISSN 1521-1878 ; 0265-9247
    ISSN (online) 1521-1878
    ISSN 0265-9247
    DOI 10.1002/bies.20390
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Aberrant and alternative splicing in cancer.

    Venables, Julian P

    Cancer research

    2004  Volume 64, Issue 21, Page(s) 7647–7654

    Abstract: Pre-mRNA splicing is a sophisticated and ubiquitous nuclear process, which is a natural source of cancer-causing errors in gene expression. Intronic splice site mutations of tumor suppressor genes often cause exon-skipping events that truncate proteins ... ...

    Abstract Pre-mRNA splicing is a sophisticated and ubiquitous nuclear process, which is a natural source of cancer-causing errors in gene expression. Intronic splice site mutations of tumor suppressor genes often cause exon-skipping events that truncate proteins just like classical nonsense mutations. Also, many studies over the last 20 years have reported cancer-specific alternative splicing in the absence of genomic mutations. Affected proteins include transcription factors, cell signal transducers, and components of the extracellular matrix. Antibodies against alternatively spliced products on cancer cells are currently in clinical trials, and competitive reverse transcription-PCR across regions of alternative splicing is being used as a simple diagnostic test. As well as being associated with cancer, the nature of the alternative gene products is usually consistent with an active role in cancer; therefore, the alternative splicing process itself is a potential target for gene therapy.
    MeSH term(s) Alternative Splicing ; Extracellular Matrix Proteins/genetics ; Humans ; Membrane Proteins/genetics ; Neoplasms/etiology ; Neoplasms/genetics ; Neoplasms/therapy ; Transcription Factors/genetics
    Chemical Substances Extracellular Matrix Proteins ; Membrane Proteins ; Transcription Factors
    Language English
    Publishing date 2004-11-01
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 1432-1
    ISSN 1538-7445 ; 0008-5472
    ISSN (online) 1538-7445
    ISSN 0008-5472
    DOI 10.1158/0008-5472.CAN-04-1910
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: Alternative splicing in the testes.

    Venables, Julian P

    Current opinion in genetics & development

    2002  Volume 12, Issue 5, Page(s) 615–619

    Abstract: Germ-cell differentiation is an ideal process for studying the effects of alternative splicing and there are examples of alternative splicing of genes involved in gene regulation and signal transduction at every stage of the spermatogenic pathway. A ... ...

    Abstract Germ-cell differentiation is an ideal process for studying the effects of alternative splicing and there are examples of alternative splicing of genes involved in gene regulation and signal transduction at every stage of the spermatogenic pathway. A network of testes-specific splicing factor interactions has been uncovered and combining our knowledge of these RNAs and proteins should lead to an understanding of the regulation of alternative splicing and male fertility.
    MeSH term(s) Alternative Splicing ; Cell Differentiation ; Fertility/genetics ; Forecasting ; Gene Expression Regulation ; Humans ; Male ; Nuclear Proteins/genetics ; RNA Precursors ; Sex Differentiation ; Spermatogenesis ; Testis/cytology ; Testis/embryology ; Testis/physiology
    Chemical Substances Nuclear Proteins ; RNA Precursors
    Language English
    Publishing date 2002-10
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 1077312-5
    ISSN 1879-0380 ; 0959-437X
    ISSN (online) 1879-0380
    ISSN 0959-437X
    DOI 10.1016/s0959-437x(02)00347-7
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 3240: Show issues Location:
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  8. Book: Alternative splicing in cancer, 2006

    Venables, Julian P

    2006  

    Author's details editor, Julian P. Venables
    MeSH term(s) Neoplasms/genetics ; Alternative Splicing ; Gene Expression Regulation
    Language English
    Size 309 p. :, ill.
    Publisher Transworld Research Network
    Publishing place Trivandrum, Kerala, India
    Document type Book
    ISBN 9788178952352 ; 8178952351
    Database Catalogue of the US National Library of Medicine (NLM)

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  9. Article ; Online: Regulated functional alternative splicing in Drosophila.

    Venables, Julian P / Tazi, Jamal / Juge, François

    Nucleic acids research

    2011  Volume 40, Issue 1, Page(s) 1–10

    Abstract: Alternative splicing expands the coding capacity of metazoan genes, and it was largely genetic studies in the fruit-fly Drosophila melanogaster that established the principle that regulated alternative splicing results in tissue- and stage-specific ... ...

    Abstract Alternative splicing expands the coding capacity of metazoan genes, and it was largely genetic studies in the fruit-fly Drosophila melanogaster that established the principle that regulated alternative splicing results in tissue- and stage-specific protein isoforms with different functions in development. Alternative splicing is particularly prominent in germ cells, muscle and the central nervous system where it modulates the expression of various proteins including cell-surface molecules and transcription factors. Studies in flies have given us numerous insights into alternative splicing in terms of upstream regulation, the exquisite diversity of their forms and the key differential cellular functions of alternatively spliced gene products. The current inundation of transcriptome sequencing data from Drosophila provides an unprecedented opportunity to gain a comprehensive view of alternative splicing.
    MeSH term(s) Alternative Splicing ; Animals ; Brain/metabolism ; Drosophila/genetics ; Drosophila/metabolism ; Muscles/metabolism ; Sex Factors ; Transcription Factors/genetics
    Chemical Substances Transcription Factors
    Language English
    Publishing date 2011-09-08
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 186809-3
    ISSN 1362-4962 ; 1362-4954 ; 0301-5610 ; 0305-1048
    ISSN (online) 1362-4962 ; 1362-4954
    ISSN 0301-5610 ; 0305-1048
    DOI 10.1093/nar/gkr648
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: EASI--enrichment of alternatively spliced isoforms.

    Venables, Julian P / Burn, John

    Nucleic acids research

    2006  Volume 34, Issue 15, Page(s) e103

    Abstract: Alternative splicing produces more than one protein from the majority of genes and the rarer forms can have dominant functions. Instability of alternative transcripts can also hinder the study of regulation of gene expression by alternative splicing. To ... ...

    Abstract Alternative splicing produces more than one protein from the majority of genes and the rarer forms can have dominant functions. Instability of alternative transcripts can also hinder the study of regulation of gene expression by alternative splicing. To investigate the true extent of alternative splicing we have developed a simple method of enriching alternatively spliced isoforms (EASI) from PCRs using beads charged with Thermus aquaticus single-stranded DNA-binding protein (T.Aq ssb). This directly purifies the single-stranded regions of heteroduplexes between alternative splices formed in the PCR, enabling direct sequencing of all the rare alternative splice forms of any gene. As a proof of principle the alternative transcripts of three tumour suppressor genes, TP53, MLH1 and MSH2, were isolated from testis cDNA. These contain missing exons, cryptic splice sites or include completely novel exons. EASI beads are stable for months in the fridge and can be easily combined with standard protocols to speed the cloning of novel transcripts.
    MeSH term(s) Alternative Splicing ; Biotechnology/methods ; DNA, Bacterial/isolation & purification ; DNA, Bacterial/metabolism ; DNA-Binding Proteins/metabolism ; Thermus/genetics
    Chemical Substances DNA, Bacterial ; DNA-Binding Proteins
    Language English
    Publishing date 2006
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2205588-5
    ISSN 1362-4962 ; 1746-8272 ; 0305-1048 ; 0261-3166
    ISSN (online) 1362-4962 ; 1746-8272
    ISSN 0305-1048 ; 0261-3166
    DOI 10.1093/nar/gkl592
    Database MEDical Literature Analysis and Retrieval System OnLINE

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