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Article ; Online: Comparison of N-Glycopeptide to Released N-Glycan Abundances and the Influence of Glycopeptide Mass and Charge States on N-Linked Glycosylation of IgG Antibodies.

Remoroza, Concepcion A / Burke, Meghan C / Mak, Tytus D / Sheetlin, Sergey L / Mirokhin, Yuri A / Cooper, Brian T / Goecker, Zachary C / Lowenthal, Mark S / Yang, Xiaoyu / Wang, Guanghui / Tchekhovskoi, Dmitrii V / Stein, Stephen E

Journal of proteome research

2024 Volume 23, Issue 4, Page(s) 1443–1457

Abstract: We report the comparison of mass-spectral-based abundances of tryptic glycopeptides to fluorescence abundances of released labeled glycans and the effects of mass and charge state and in-source fragmentation on glycopeptide abundances. The primary ... ...

Abstract	We report the comparison of mass-spectral-based abundances of tryptic glycopeptides to fluorescence abundances of released labeled glycans and the effects of mass and charge state and in-source fragmentation on glycopeptide abundances. The primary glycoforms derived from Rituximab, NISTmAb, Evolocumab, and Infliximab were high-mannose and biantennary complex galactosylated and fucosylated N-glycans. Except for Evolocumab, in-source ions derived from the loss of HexNAc or HexNAc-Hex sugars are prominent for other therapeutic IgGs. After excluding in-source fragmentation of glycopeptide ions from the results, a linear correlation was observed between fluorescently labeled N-glycan and glycopeptide abundances over a dynamic range of 500. Different charge states of human IgG-derived glycopeptides containing a wider variety of abundant attached glycans were also investigated to examine the effects of the charge state on ion abundances. These revealed a linear dependence of glycopeptide abundance on the mass of the glycan with higher charge states favoring higher-mass glycans. Findings indicate that the mass spectrometry-based bottom-up approach can provide results as accurate as those of glycan release studies while revealing the origin of each attached glycan. These site-specific relative abundances are conveniently displayed and compared using previously described glycopeptide abundance distribution spectra "GADS" representations. Mass spectrometry data are available from the MAssIVE repository (MSV000093562).
MeSH term(s)	Humans ; Glycosylation ; Immunoglobulin G ; Tandem Mass Spectrometry ; Glycopeptides/analysis ; Polysaccharides/chemistry ; Ions
Chemical Substances	Immunoglobulin G ; Glycopeptides ; Polysaccharides ; Ions
Language	English
Publishing date	2024-03-07
Publishing country	United States
Document type	Journal Article
ZDB-ID	2078618-9
ISSN	1535-3907 ; 1535-3893
ISSN (online)	1535-3907
ISSN	1535-3893
DOI	10.1021/acs.jproteome.3c00904
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Article ; Online: Factors and Outcomes Associated With Viral Suppression Trajectory Group Membership Among Youth Transitioning From Pediatric to Adult HIV Care.

Nassau, Tanner / Loabile, Bogadi / Dowshen, Nadia / Lowenthal, Elizabeth / Conway, Daniel / Brady, Kathleen A / Momplaisir, Florence M

The Journal of adolescent health : official publication of the Society for Adolescent Medicine

2022 Volume 71, Issue 6, Page(s) 737–743

Abstract: Purpose: Youth experiencing healthcare transition (HCT) from pediatric to adult HIV care are at risk for poor viral suppression (VS). We have a limited understanding of VS trajectory groups (VSTGs) pre- and post-HCT and factors associated with these ... ...

Abstract	Purpose: Youth experiencing healthcare transition (HCT) from pediatric to adult HIV care are at risk for poor viral suppression (VS). We have a limited understanding of VS trajectory groups (VSTGs) pre- and post-HCT and factors associated with these trajectories. Methods: We analyzed Philadelphia HIV surveillance data of youth diagnosed with HIV at least 2 years pre-HCT. We used group-based trajectory analysis to characterize VS trends pre- and post-HCT. We compared baseline sociodemographic characteristics across the different VSTGs and care continuum outcomes in the year post-HCT. Generalized estimating equations evaluated the association between VSTG and HIV care continuum outcomes measured 2 years post-HCT. Results: Between 2012 and 2019, 232 eligible youth underwent HCT: 69.4% were aged 24-25, 75.4% male, and 76.7% non-Hispanic Black. Three VSTGs were identified: low (30.6%), increasing (26.7%), and high probability (42.7%) for VS. Younger age was associated with high-probability VSTG membership: 59.2% of those aged 18-23 versus 35.4% of those aged 24-25 were in the high-probability VSTG (p < .001). Demographics found to be associated with linkage to care post-HCT included younger age (p = .018), female sex at birth (p = .038), and perinatal acquisition (p = .012). Perinatal acquisition was also associated with retention in care in the year post-HCT (p = .029). For those transitioning between 2012 and 2018, those in the high-probability VSTG had greater odds of being retained (adjusted odds ratio 1.68, 95% confidence interval 1.03-2.71) and VS (adjusted odds ratio 6.95, interval 3.74-12.95) 2 years post-HCT, compared to those in the low VSTG. Discussion: We identified distinct VSTGs that informed long-term trends post-HCT. VSTG membership may allow for tailoring of appropriate HCT support.
MeSH term(s)	Adult ; Pregnancy ; Infant, Newborn ; Child ; Humans ; Adolescent ; Male ; Female ; Transition to Adult Care ; HIV Infections/epidemiology ; Black People ; Group Processes ; Philadelphia/epidemiology ; Viral Load
Language	English
Publishing date	2022-10-08
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	1063374-1
ISSN	1879-1972 ; 1054-139X
ISSN (online)	1879-1972
ISSN	1054-139X
DOI	10.1016/j.jadohealth.2022.07.009
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Article ; Online: Impact of COVID-19 on Adolescent HIV Prevention and Treatment Research in the AHISA Network.

Lowenthal, Elizabeth D / DeLong, Stephanie M / Zanoni, Brian / Njuguna, Irene / Beima-Sofie, Kristin / Dow, Dorothy / Shayo, Aisa / Schreibman, Alana / Ahmed, Charisse V / Chapman, Jennifer / Chen, Lydia / Mehta, Shreya / Mbizvo, Michael T

AIDS and behavior

2022 Volume 27, Issue Suppl 1, Page(s) 73–83

Abstract: Members of the Adolescent HIV Prevention and Treatment Implementation Science Alliance (AHISA) network conduct research aiming to close gaps between what is known to be impactful across the HIV prevention and treatment cascade, and services delivered to ... ...

Abstract	Members of the Adolescent HIV Prevention and Treatment Implementation Science Alliance (AHISA) network conduct research aiming to close gaps between what is known to be impactful across the HIV prevention and treatment cascade, and services delivered to optimize outcomes for adolescents/young adults (AYA) in high HIV-prevalence settings. The COVID-19 pandemic introduced new challenges which threaten to exacerbate care and access disparities. We report results of a survey among AHISA teams with active AYA HIV research programs in African countries to determine how the pandemic has impacted their efforts. Results highlighted the detrimental impact of the pandemic on research efforts and the expanded need for implementation research to help provide evidence-based, context-specific pandemic recovery support. Key lessons learned included the viability of remote service delivery strategies and other innovations, the need for adaptive systems that respond to evolving contextual needs, and the need for organized documentation plans, within empathic and flexible environments.
MeSH term(s)	Young Adult ; Humans ; Adolescent ; HIV Infections/epidemiology ; HIV Infections/prevention & control ; Pandemics ; COVID-19/prevention & control ; Acquired Immunodeficiency Syndrome/prevention & control ; Africa
Language	English
Publishing date	2022-09-12
Publishing country	United States
Document type	Journal Article
ZDB-ID	1339885-4
ISSN	1573-3254 ; 1090-7165
ISSN (online)	1573-3254
ISSN	1090-7165
DOI	10.1007/s10461-022-03811-5
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Article ; Online: TB and TB-HIV care for adolescents and young adults.

Enane, L A / Eby, J / Arscott-Mills, T / Argabright, S / Caiphus, C / Kgwaadira, B / Steenhoff, A P / Lowenthal, E D

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease

2020 Volume 24, Issue 2, Page(s) 240–249

Abstract: SETTING: ...

Abstract	SETTING:
MeSH term(s)	Adolescent ; Adult ; Child ; Humans ; Young Adult ; Botswana ; Counseling ; Health Personnel ; HIV Infections/diagnosis ; HIV Infections/therapy ; Social Stigma ; Tuberculosis/diagnosis ; Tuberculosis/therapy
Language	English
Publishing date	2020-03-03
Publishing country	France
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	1385624-8
ISSN	1815-7920 ; 1027-3719
ISSN (online)	1815-7920
ISSN	1027-3719
DOI	10.5588/ijtld.19.0416
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Zs.A 4776: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MO 261: Show issues

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Article ; Online: Towards quality adolescent-friendly services in TB care.

Laycock, K M / Eby, J / Arscott-Mills, T / Argabright, S / Caiphus, C / Kgwaadira, B / Lowenthal, E D / Steenhoff, A P / Enane, L A

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease

2021 Volume 25, Issue 7, Page(s) 579–583

MeSH term(s)	Adolescent ; Humans ; Health Services Accessibility ; Quality of Health Care ; Tuberculosis/therapy
Language	English
Publishing date	2021-06-25
Publishing country	France
Document type	Letter ; Research Support, N.I.H., Extramural
ZDB-ID	1385624-8
ISSN	1815-7920 ; 1027-3719
ISSN (online)	1815-7920
ISSN	1027-3719
DOI	10.5588/ijtld.21.0059
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Zs.MO 261: Show issues

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Article ; Online: Reasons for discordance between antiretroviral adherence measures in adolescents.

Lowenthal, Elizabeth D / Ohrenshall, Rachel / Moshashane, Neo / Bula, Boineelo / Chapman, Jennifer / Marukutira, Tafireyi / Tshume, Ontibile / Gross, Robert / Mphele, Seipone

AIDS care

2021 Volume 34, Issue 9, Page(s) 1135–1143

Abstract: Adolescents with suboptimal medication taking may deceive caregivers about non-adherence. We conducted a 5-year longitudinal study of adolescents monitored simultaneously with both easily manipulated (e.g., self-report) and hard to manipulate (e.g., ... ...

Abstract	Adolescents with suboptimal medication taking may deceive caregivers about non-adherence. We conducted a 5-year longitudinal study of adolescents monitored simultaneously with both easily manipulated (e.g., self-report) and hard to manipulate (e.g., microelectronic data) strategies. Adolescents with repeatedly high adherence on the former and low adherence on the latter were invited along with their parental figures ("parents") to participate. We conducted focus groups and semi-structured interviews, separately for adolescents and parents, to elucidate drivers of discordant measures. Forty-seven adolescents and 26 parents participated in focus groups and 4 adolescents were interviewed. Adolescents described hiding pills, discarding pills, and lying. Their motivations included fear of disappointing those who care about them, desire to avoid admonishment by parents and clinic staff, and desire to avoid remedial adherence counseling. Both adolescents and parents considered negative feedback for prior poor adherence to be key motivation to hide current poor adherence from clinic staff. Providing positive feedback for truth-telling, rather than for "evidence" of excellent adherence, might help adolescent patients and their parents to develop stronger treatment alliances with each other and with clinic staff. Such alliances would allow adherence interventions to be better targeted and more fruitful in increasing adherence.
MeSH term(s)	Adolescent ; Anti-Retroviral Agents/therapeutic use ; Caregivers ; HIV Infections/drug therapy ; HIV Infections/psychology ; Humans ; Longitudinal Studies ; Medication Adherence/psychology
Chemical Substances	Anti-Retroviral Agents
Language	English
Publishing date	2021-08-23
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	1012651-x
ISSN	1360-0451 ; 0954-0121
ISSN (online)	1360-0451
ISSN	0954-0121
DOI	10.1080/09540121.2021.1968998
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Article ; Online: Lead metal biosorption and isotherms studies by metal-resistant Bacillus strain MRS-2 bacterium.

Hoyle-Gardner, Jada / Jones, Winslow / Badisa, Veera L D / Mwashote, Benjamin / Ibeanusi, Victor / Gaines, Treasure / Lowenthal, Hannah / Tucker, Landon

Journal of basic microbiology

2021 Volume 61, Issue 8, Page(s) 697–708

Abstract: In this study, lead (Pb) biosorption studies in aqueous solution were performed with metal-resistant Bacillus strain MRS-2 (ATCC 55674) bacterium which was previously isolated from wastewater plant. It showed minimum inhibition concentration of 300 ppm ... ...

Abstract	In this study, lead (Pb) biosorption studies in aqueous solution were performed with metal-resistant Bacillus strain MRS-2 (ATCC 55674) bacterium which was previously isolated from wastewater plant. It showed minimum inhibition concentration of 300 ppm Pb on the nutrient agar plates. Pb biosorption using MRS-2 bacteria was investigated under different parameters such as pH, temperature, biomass dosage, initial Pb concentration, contact time, and type of biomass by batch experiments. Pb concentration was analyzed through Inductively coupled plasma-optical emission spectrometry. The rate of biosorption (Q) and Pb biosorption capacity (q
MeSH term(s)	Adsorption ; Bacillus/drug effects ; Bacillus/physiology ; Biomass ; Drug Resistance, Bacterial/physiology ; Hydrogen-Ion Concentration ; Lead/pharmacology ; Metals/pharmacology ; Microbial Sensitivity Tests ; Temperature ; Waste Water
Chemical Substances	Metals ; Waste Water ; Lead (2P299V784P)
Language	English
Publishing date	2021-07-06
Publishing country	Germany
Document type	Journal Article
ZDB-ID	632513-0
ISSN	1521-4028 ; 0233-111X
ISSN (online)	1521-4028
ISSN	0233-111X
DOI	10.1002/jobm.202100155
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Article ; Online: Impact of COVID-19 on Adolescent HIV Prevention and Treatment Services in the AHISA Network.

Ahmed, Charisse V / Brooks, Merrian J / DeLong, Stephanie M / Zanoni, Brian C / Njuguna, Irene / Beima-Sofie, Kristin / Dow, Dorothy E / Shayo, Aisa / Schreibman, Alana / Chapman, Jennifer / Chen, Lydia / Mehta, Shreya / Mbizvo, Michael T / Lowenthal, Elizabeth D

AIDS and behavior

2022 Volume 27, Issue Suppl 1, Page(s) 84–93

Abstract: We investigated perceived impacts of COVID-19 on the delivery of adolescent HIV treatment and prevention services in sub-Saharan Africa (SSA) by administering a survey to members of the Adolescent HIV Prevention and Treatment Implementation Science ... ...

Abstract	We investigated perceived impacts of COVID-19 on the delivery of adolescent HIV treatment and prevention services in sub-Saharan Africa (SSA) by administering a survey to members of the Adolescent HIV Prevention and Treatment Implementation Science Alliance (AHISA) from February to April 2021. We organized COVID-19 impacts, as perceived by AHISA teams, under three themes: service interruptions, service adjustments, and perceived individual-level health impacts. AHISA teams commonly reported interruptions to prevention programs, diagnostic testing, and access to antiretroviral therapy (ART). Common service adjustments included decentralization of ART refills, expanded multi-month ART distribution, and digital technology use. Perceived individual-level impacts included social isolation, loss to follow-up, food insecurity, poverty, and increases in adolescent pregnancies and sexually transmitted infections. The need for collaboration among stakeholders were commonly cited as lessons learned by AHISA teams. Survey findings highlight the need for implementation science research to evaluate the effects of pandemic-related HIV service adaptations in SSA.
MeSH term(s)	Pregnancy ; Female ; Humans ; Adolescent ; COVID-19/prevention & control ; HIV Infections/drug therapy ; HIV Infections/epidemiology ; HIV Infections/prevention & control ; Acquired Immunodeficiency Syndrome/drug therapy ; Health Services Accessibility ; Anti-Retroviral Agents/therapeutic use
Chemical Substances	Anti-Retroviral Agents
Language	English
Publishing date	2022-12-27
Publishing country	United States
Document type	Journal Article
ZDB-ID	1339885-4
ISSN	1573-3254 ; 1090-7165
ISSN (online)	1573-3254
ISSN	1090-7165
DOI	10.1007/s10461-022-03959-0
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Article ; Online: How collaboration between bioethicists and neuroscientists can advance research.

Nature neuroscience

2022 Volume 25, Issue 11, Page(s) 1399–1401

MeSH term(s)	Humans ; Ethicists
Language	English
Publishing date	2022-10-18
Publishing country	United States
Document type	Journal Article
ZDB-ID	1420596-8
ISSN	1546-1726 ; 1097-6256
ISSN (online)	1546-1726
ISSN	1097-6256
DOI	10.1038/s41593-022-01187-2
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Zs.A 4891: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG)
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Article ; Online: Symptoms of depression, anxiety, and thoughts of suicide/self-injury in adolescents and young adults living with HIV in Botswana.

Brooks, Merrian / Burmen, Barbara / Olashore, Anthony / Gezmu, Alemayehu Mekonnen / Molebatsi, Keneilwe / Tshume, Ontibile / Phoi, Onkemetse / Morales, Knashawn / Matshaba, Mogomotsi / Benton, Tami / Lowenthal, Elizabeth D

African journal of AIDS research : AJAR

2022 Volume 22, Issue 1, Page(s) 54–62

Abstract: Globally, mental health problems have been reported to be more common in youth living with HIV (YLWH) than in the general population, but routine mental health screening is rarely done in high-volume HIV clinics. In 2019, YLWH in a large HIV clinic in ... ...

Abstract	Globally, mental health problems have been reported to be more common in youth living with HIV (YLWH) than in the general population, but routine mental health screening is rarely done in high-volume HIV clinics. In 2019, YLWH in a large HIV clinic in Botswana were screened using the Generalized Anxiety Scale-7 (GAD-7) and Patient Health Questionnaire-9 (PHQ-9) in a pilot standard-of-care screening programme. Two-way ANOVA was used to describe the effects of age group (12-<16, 16-<20 and 20-25 years old) and sex on GAD-7 and PHQ-9 scores. Chi-square statistics were used to compare characteristics of YLWH with and without potential suicidality/self-harm symptoms based on question 9 in the PHQ-9. Among 1 469 YLWH, 33.1%, 44.3% and 15.0% had anxiety, depression and potential suicidality/self-harm symptoms respectively. YLWH of 20-25 years old and 16-<20 years old had higher GAD-7 scores compared to 12-<16-year-olds (
MeSH term(s)	Male ; Humans ; Adolescent ; Female ; Young Adult ; Adult ; Depression/epidemiology ; Depression/psychology ; Botswana/epidemiology ; HIV Infections/epidemiology ; HIV Infections/psychology ; Anxiety/epidemiology ; Anxiety/psychology ; Self-Injurious Behavior/epidemiology ; Suicide
Language	English
Publishing date	2022-12-28
Publishing country	South Africa
Document type	Journal Article
ZDB-ID	2148999-3
ISSN	1727-9445 ; 1608-5906
ISSN (online)	1727-9445
ISSN	1608-5906
DOI	10.2989/16085906.2023.2186252
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