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  1. Article ; Online: Global analysis of aging-related protein structural changes uncovers enzyme-polymerization-based control of longevity.

    Paukštytė, Jurgita / López Cabezas, Rosa María / Feng, Yuehan / Tong, Kai / Schnyder, Daniela / Elomaa, Ellinoora / Gregorova, Pavlina / Doudin, Matteo / Särkkä, Meeri / Sarameri, Jesse / Lippi, Alice / Vihinen, Helena / Juutila, Juhana / Nieminen, Anni / Törönen, Petri / Holm, Liisa / Jokitalo, Eija / Krisko, Anita / Huiskonen, Juha /
    Sarin, L Peter / Hietakangas, Ville / Picotti, Paola / Barral, Yves / Saarikangas, Juha

    Molecular cell

    2023  Volume 83, Issue 18, Page(s) 3360–3376.e11

    Abstract: Aging is associated with progressive phenotypic changes. Virtually all cellular phenotypes are produced by proteins, and their structural alterations can lead to age-related diseases. However, we still lack comprehensive knowledge of proteins undergoing ... ...

    Abstract Aging is associated with progressive phenotypic changes. Virtually all cellular phenotypes are produced by proteins, and their structural alterations can lead to age-related diseases. However, we still lack comprehensive knowledge of proteins undergoing structural-functional changes during cellular aging and their contributions to age-related phenotypes. Here, we conducted proteome-wide analysis of early age-related protein structural changes in budding yeast using limited proteolysis-mass spectrometry (LiP-MS). The results, compiled in online ProtAge catalog, unraveled age-related functional changes in regulators of translation, protein folding, and amino acid metabolism. Mechanistically, we found that folded glutamate synthase Glt1 polymerizes into supramolecular self-assemblies during aging, causing breakdown of cellular amino acid homeostasis. Inhibiting Glt1 polymerization by mutating the polymerization interface restored amino acid levels in aged cells, attenuated mitochondrial dysfunction, and led to lifespan extension. Altogether, this comprehensive map of protein structural changes enables identifying mechanisms of age-related phenotypes and offers opportunities for their reversal.
    MeSH term(s) Longevity/genetics ; Polymerization ; Cellular Senescence ; Amino Acids
    Chemical Substances Amino Acids
    Language English
    Publishing date 2023-09-11
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1415236-8
    ISSN 1097-4164 ; 1097-2765
    ISSN (online) 1097-4164
    ISSN 1097-2765
    DOI 10.1016/j.molcel.2023.08.015
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

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  2. Article: Drug transport to brain with targeted liposomes.

    Schnyder, Anita / Huwyler, Jörg

    NeuroRx : the journal of the American Society for Experimental NeuroTherapeutics

    2005  Volume 2, Issue 1, Page(s) 99–107

    Abstract: Antibody-conjugated liposomes or immunoliposomes are particulate drug carriers that can be used to direct encapsulated drug molecules to diseased tissues or organs. The present review discusses examples of successful applications of this technology to ... ...

    Abstract Antibody-conjugated liposomes or immunoliposomes are particulate drug carriers that can be used to direct encapsulated drug molecules to diseased tissues or organs. The present review discusses examples of successful applications of this technology to achieve drug transport across the blood-brain barrier. In addition, information will be provided on practical aspects such as phospholipid compositions of liposomes, antibody coupling technologies, large-scale production of liposomes, and obstacles related to drug loading of the carrier. Prospects of future uses of immunoliposome-based drug delivery systems such as gene therapy of the brain and clinical trials are discussed.
    MeSH term(s) Animals ; Brain/metabolism ; Drug Delivery Systems ; Humans ; Immunochemistry ; Liposomes/chemistry ; Pharmacokinetics
    Chemical Substances Liposomes
    Language English
    Publishing date 2005-02-16
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 2205033-4
    ISSN 1545-5351 ; 1545-5343
    ISSN (online) 1545-5351
    ISSN 1545-5343
    DOI 10.1602/neurorx.2.1.99
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Book ; Online ; Thesis: Synthesis of pyrazole-containing ferrocenyl ligands and their application in the rhodium-catalyzed hydroboration of olefins

    Schnyder, Anita

    1996  

    Author's details by Anita Schnyder
    Language English
    Size Online-Ressource (XXII, 180 S), Ill
    Publishing place Zürich
    Document type Book ; Online ; Thesis
    Thesis / German Habilitation thesis Diss. Naturwiss. ETH Zürich, Nr. 11724. Ref.: A. Togni ; Korref.: A. Pfaltz--Zürich, 1172
    Database Former special subject collection: coastal and deep sea fishing

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  4. Book ; Online ; Thesis: Synthesis of pyrazole-containing ferrocenyl ligands and their application in the rhodium-catalyzed hydroboration of olefins

    Schnyder, Anita

    1996  

    Author's details by Anita Schnyder
    Language English
    Size Online-Ressource (XXII, 180 S), Ill
    Publishing place Zürich
    Document type Book ; Online ; Thesis
    Thesis / German Habilitation thesis Diss. Naturwiss. ETH Zürich, Nr. 11724. Ref.: A. Togni ; Korref.: A. Pfaltz--Zürich, 1172
    Database Library catalogue of the German National Library of Science and Technology (TIB), Hannover

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  5. Book ; Thesis: Synthesis of pyrazole-containing ferrocenyl ligands and their application in the rhodium-catalyzed hydroboration of olefins

    Schnyder, Anita

    1996  

    Author's details by Anita Schnyder
    Language English
    Size 3 Mikrofiches, graph. Darst
    Document type Book ; Thesis
    Thesis / German Habilitation thesis @Zürich, Eidgen. Techn. Hochsch., Diss., 1996
    Database Library catalogue of the German National Library of Science and Technology (TIB), Hannover

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  6. Book ; Online: Synthesis of pyrazole-containing ferrocenyl ligands and their application in the rhodium-catalyzed hydroboration of olefins

    Schnyder, Anita

    1996  

    Abstract: Diss. Naturwiss. ETH Zürich, Nr. 11724, 1996. Ref.: A. Togni ... Korref.: A. ... ...

    Abstract Diss. Naturwiss. ETH Zürich, Nr. 11724, 1996. Ref.: A. Togni

    Korref.: A. Pfaltz
    Keywords CHEMISCHE SYNTHESEN + CHEMISCHE REAKTIONEN ; PYRAZOL UND DERIVATE (HETEROCYCLISCHE KOHLENWASSERSTOFFE) ; EISENKOMPLEXE (KOMPLEXCHEMIE) ; CYCLOPENTADIENYLKOMPLEXE (KOMPLEXCHEMIE) ; RHODIUMKATALYSATOREN (CHEMISCHE REAKTIONEN) ; ALKENE (ORGANISCHE CHEMIE) ; CHELATLIGANDEN + CHELATKOMPLEXE (KOMPLEXCHEMIE) ; HYDROBORIERUNG (CHEMISCHE REAKTIONEN)
    Language English
    Publisher Zürich
    Publishing country ch
    Document type Book ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

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  7. Article: Synthesis of unsymmetrical aroyl acyl imides by aminocarbonylation of aryl bromides.

    Schnyder, Anita / Indolese, Adriano F

    The Journal of organic chemistry

    2002  Volume 67, Issue 2, Page(s) 594–597

    Abstract: Aroyl imides were prepared by a palladium-catalyzed aminocarbonylation reaction of aryl bromides with carbon monoxide and primary amides in good yields (58-72%). The reactions were carried out under mild conditions (5 bar, 120 degrees C) using 1 mol % of ...

    Abstract Aroyl imides were prepared by a palladium-catalyzed aminocarbonylation reaction of aryl bromides with carbon monoxide and primary amides in good yields (58-72%). The reactions were carried out under mild conditions (5 bar, 120 degrees C) using 1 mol % of a palladium phosphine complex. Several aryl bromides were reacted with formamide, acetamide, benzamide, and benzenesulfonamide, respectively. For activated aryl bromides, a phosphine-to-palladium ratio of 2:1 was sufficient, but less reactive aryl bromides required a ligand-to-palladium ratio of 6:1 in order to stabilize the catalyst and achieve full conversion. The imides were very sensitive to aqueous basic conditions and were easily converted to aroyl amides or benzoic acids.
    Language English
    Publishing date 2002-01-25
    Publishing country United States
    Document type Journal Article
    ZDB-ID 123490-0
    ISSN 1520-6904 ; 0022-3263
    ISSN (online) 1520-6904
    ISSN 0022-3263
    DOI 10.1021/jo016076a
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 4473: Show issues Location:
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  8. Article: Targeting of daunomycin using biotinylated immunoliposomes: pharmacokinetics, tissue distribution and in vitro pharmacological effects.

    Schnyder, Anita / Krähenbühl, Stephan / Drewe, Jürgen / Huwyler, Jörg

    Journal of drug targeting

    2005  Volume 13, Issue 5, Page(s) 325–335

    Abstract: Biotinylated immunoliposomes were prepared by a non-covalent (biotin-streptavidin) coupling procedure and conjugated to the OX26 monoclonal antibody directed against the rat transferrin receptor. In vitro, these biotinylated immunoliposomes were used to ... ...

    Abstract Biotinylated immunoliposomes were prepared by a non-covalent (biotin-streptavidin) coupling procedure and conjugated to the OX26 monoclonal antibody directed against the rat transferrin receptor. In vitro, these biotinylated immunoliposomes were used to by-pass P-glycoprotein in multidrug-resistant RBE4 brain capillary endothelial cells and thereby to achieve 2- to 3-fold higher intracellular accumulation of liposomal daunomycin as compared to free drug. The extent of cellular uptake of liposomal daunomycin was dose- and time-dependent, was inhibited by competition with unbound OX26 and was associated with a pharmacological (i.e. cytotoxic) effect. Cytotoxic effects of liposomal formulations of daunomycin, in contrast to the free drug, were apparent only after prolonged incubation periods being indicative of a slow intracellular unpacking and release of liposomal daunomycin. Pharmacokinetics and tissue distribution studies in the rat revealed brain accumulation of daunomycin in OX26-immunoliposomes to higher levels as compared to brain uptake of free daunomycin, or daunomycin incorporated within pegylated liposomes or within unspecific IgG(2a) isotype control immunoliposomes. Such OX26-mediated effects were not observed in other tissues such as spleen, liver, muscle or kidney.
    MeSH term(s) Algorithms ; Animals ; Anti-Bacterial Agents/administration & dosage ; Anti-Bacterial Agents/pharmacokinetics ; Anti-Bacterial Agents/pharmacology ; Antibodies, Monoclonal/pharmacology ; Biotin ; Cells, Cultured ; Daunorubicin/administration & dosage ; Daunorubicin/pharmacokinetics ; Daunorubicin/pharmacology ; Dose-Response Relationship, Drug ; Drug Carriers ; Drug Delivery Systems ; Immunochemistry ; Immunoglobulin G/immunology ; Liposomes ; Male ; Rats ; Rats, Wistar ; Rhodamines/toxicity ; Tissue Distribution
    Chemical Substances Anti-Bacterial Agents ; Antibodies, Monoclonal ; Drug Carriers ; Immunoglobulin G ; Liposomes ; Rhodamines ; lissamine rhodamine B (2609-88-3) ; Biotin (6SO6U10H04) ; Daunorubicin (ZS7284E0ZP)
    Language English
    Publishing date 2005-09-29
    Publishing country England
    Document type Journal Article
    ZDB-ID 1187110-6
    ISSN 1029-2330 ; 1061-186X
    ISSN (online) 1029-2330
    ISSN 1061-186X
    DOI 10.1080/10611860500206674
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 4481: Show issues Location:
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  9. Article: A new generation of air stable, highly active Pd complexes for C--C and C--N coupling reactions with aryl chlorides.

    Schnyder, Anita / Indolese, Adriano F / Studer, Martin / Blaser, Hans-Ulrich

    Angewandte Chemie (International ed. in English)

    2002  Volume 41, Issue 19, Page(s) 3668–71, 3520

    Language English
    Publishing date 2002-10-04
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2011836-3
    ISSN 1521-3773 ; 1433-7851
    ISSN (online) 1521-3773
    ISSN 1433-7851
    DOI 10.1002/1521-3773(20021004)41:19<3668::AID-ANIE3668>3.0.CO;2-U
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  10. Article ; Online: Targeting of skeletal muscle in vitro using biotinylated immunoliposomes.

    Schnyder, Anita / Krähenbühl, Stefan / Török, Michael / Drewe, Jürgen / Huwyler, Jörg

    The Biochemical journal

    2004  Volume 377, Issue Pt 1, Page(s) 61–67

    Abstract: In the present study, a non-covalent (biotin-streptavidin) coupling procedure for the preparation of pegylated immunoliposomes is presented, which simplifies the attachment of targeting vectors to sterically stabilized liposomes. A biotinylated poly( ... ...

    Abstract In the present study, a non-covalent (biotin-streptavidin) coupling procedure for the preparation of pegylated immunoliposomes is presented, which simplifies the attachment of targeting vectors to sterically stabilized liposomes. A biotinylated poly(ethylene glycol) (PEG)-phospholipid [bio-PEG-distearoylphosphatidylethanolamine (DSPE)] was used as a linker between a streptavidin-conjugated monoclonal antibody (mAb) (i.e. the OX26 mAb raised against the rat transferrin receptor) and 150 nm liposomes. OX26-streptavidin had a biotin binding capacity of two to three biotin molecules per OX26-streptavidin conjugate. Immunostaining experiments with the OX26 mAb followed by fluorescent confocal microscopy revealed immunofluorescence labelling of the transferrin receptor on skeletal muscle, as well as in L6 cells, a continuous cell line derived from rat skeletal muscle. Uptake experiments with L6 cells using the OX26 mAb, fluorescence-labelled OX26-streptavidin or fluorescent OX26-immunoliposomes demonstrated cellular uptake and accumulation within an intracellular compartment of the OX26 mAb and its conjugates. Cellular uptake of OX26 conjugates was sensitive to competition with free OX26 antibody. In summary, these studies describe the design of biotinylated immunoliposomes as a universal drug transport vector and their potential for targeting of the transferrin receptor of skeletal muscle.
    MeSH term(s) Animals ; Antibodies, Monoclonal/chemistry ; Antibodies, Monoclonal/immunology ; Antibodies, Monoclonal/metabolism ; Biotinylation ; Cell Line ; Drug Delivery Systems ; Liposomes/chemistry ; Microscopy, Confocal ; Microscopy, Fluorescence ; Muscle, Skeletal/chemistry ; Muscle, Skeletal/metabolism ; Phosphatidylethanolamines/chemistry ; Polyethylene Glycols/chemistry ; Rats ; Receptors, Transferrin/analysis ; Receptors, Transferrin/immunology ; Receptors, Transferrin/metabolism
    Chemical Substances Antibodies, Monoclonal ; Liposomes ; Phosphatidylethanolamines ; Receptors, Transferrin ; polyethylene glycol-distearoylphosphatidylethanolamine ; Polyethylene Glycols (30IQX730WE)
    Language English
    Publishing date 2004-01-01
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2969-5
    ISSN 1470-8728 ; 0006-2936 ; 0306-3275 ; 0264-6021
    ISSN (online) 1470-8728
    ISSN 0006-2936 ; 0306-3275 ; 0264-6021
    DOI 10.1042/BJ20031034
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    Uc I Zs.110: Show issues Location:
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