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Article ; Online: Protocol to prepare doubly labeled fluorescent nucleosomes for single-molecule fluorescence microscopy.

Ghoneim, Mohamed / Musselman, Catherine A

STAR protocols

2023 Volume 4, Issue 2, Page(s) 102229

Abstract: Single-molecule fluorescence microscopy (SMFM) has been shown to be informative in understanding the interaction of chromatin-associated factors with nucleosomes, the basic building unit of chromatin. Here, we present a protocol for preparing doubly ... ...

Abstract	Single-molecule fluorescence microscopy (SMFM) has been shown to be informative in understanding the interaction of chromatin-associated factors with nucleosomes, the basic building unit of chromatin. Here, we present a protocol for preparing doubly labeled fluorescent nucleosomes for SMFM. We describe steps for over-expression in E. coli and purification of recombinant human core histones. We then detail fluorescent labeling of histones and nucleosomal double-stranded DNA followed by octamer refolding and nucleosome reconstitution.
Language	English
Publishing date	2023-04-19
Publishing country	United States
Document type	Journal Article
ISSN	2666-1667
ISSN (online)	2666-1667
DOI	10.1016/j.xpro.2023.102229
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Article ; Online: Current status of stem cell therapy for type 1 diabetes: a critique and a prospective consideration.

Ghoneim, Mohamed A / Gabr, Mahmoud M / El-Halawani, Sawsan M / Refaie, Ayman F

Stem cell research & therapy

2024 Volume 15, Issue 1, Page(s) 23

Abstract: Over the past decade, there had been progress in the development of cell therapy for insulin-dependent diabetes. Nevertheless, important hurdles that need to be overcome still remain. Protocols for the differentiation of pluripotent stem cells into ... ...

Abstract	Over the past decade, there had been progress in the development of cell therapy for insulin-dependent diabetes. Nevertheless, important hurdles that need to be overcome still remain. Protocols for the differentiation of pluripotent stem cells into pancreatic progenitors or fully differentiated β-cells have been developed. The resulting insulin-producing cells can control chemically induced diabetes in rodents and were the subject of several clinical trials. However, these cells are immunogenic and possibly teratogenic for their transplantation, and an immunoisolation device and/or immunosuppression is needed. A growing number of studies have utilized genetic manipulations to produce immune evasive cells. Evidence must be provided that in addition to the expected benefit, gene manipulations should not lead to any unforeseen complications. Mesenchymal stem/stromal cells (MSCs) can provide a viable alternative. MSCs are widely available from many tissues. They can form insulin-producing cells by directed differentiation. Experimentally, evidence has shown that the transplantation of allogenic insulin-producing cells derived from MSCs is associated with a muted allogeneic response that does not interfere with their functionality. This can be explained by the immunomodulatory functions of the MSC subpopulation that did not differentiate into insulin-producing cells. Recently, exosomes derived from naive MSCs have been used in the experimental domain to treat diabetes in rodents with varying degrees of success. Several mechanisms for their beneficial functions were proposed including a reduction in insulin resistance, the promotion of autophagy, and an increase in the T regulatory population. However, euglycemia was not achieved in any of these experiments. We suggest that exosomes derived from β-cells or insulin-producing cells (educated) can provide a better therapeutic effect than those derived from undifferentiated cells.
MeSH term(s)	Humans ; Diabetes Mellitus, Type 1/therapy ; Diabetes Mellitus, Type 1/metabolism ; Prospective Studies ; Mesenchymal Stem Cell Transplantation/methods ; Insulin-Secreting Cells ; Cell Differentiation ; Insulin/metabolism
Chemical Substances	Insulin
Language	English
Publishing date	2024-01-29
Publishing country	England
Document type	Journal Article ; Review
ZDB-ID	2548671-8
ISSN	1757-6512 ; 1757-6512
ISSN (online)	1757-6512
ISSN	1757-6512
DOI	10.1186/s13287-024-03636-0
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Article ; Online: Endoscopic third ventriculostomy for management of hydrocephalus associated with Chiari malformation type II in children.

El-Ghandour, Nasser M F / Salama, Mohamed M / Ghoneim, Mohamed Adel / Attia, Ahmed M

Child's nervous system : ChNS : official journal of the International Society for Pediatric Neurosurgery

2023 Volume 39, Issue 6, Page(s) 1565–1571

Abstract: Background: Hydrocephalus is commonly associated with Chiari malformation (CM) particularly CM type II. The traditional treatment of hydrocephalus in these patients has been cerebrospinal fluid diversion by shunts. Endoscopic third ventriculostomy (ETV) ...

Abstract	Background: Hydrocephalus is commonly associated with Chiari malformation (CM) particularly CM type II. The traditional treatment of hydrocephalus in these patients has been cerebrospinal fluid diversion by shunts. Endoscopic third ventriculostomy (ETV) has emerged as an alternative procedure in these patients. Purpose: Assessment of the clinical and radiological outcomes of ETV in the management of hydrocephalus in children with CM II. Methods: This is a prospective study conducted on 18 patients with CM II associated with hydrocephalus admitted to Cairo University hospitals between January 2020 and June 2021. These patients had been managed surgically by ETV. Clinical outcome was assessed based on improvement of manifestations of increased intracranial pressure while radiological outcome was based on the findings of postoperative computed tomography. In cases with early failure, serial lumbar puncture (LP) was performed for 2 days. Results: ETV was performed as a secondary procedure in 4 cases. The overall success rate of the procedure was 72%, and its success rate as a secondary procedure was 100%. Serial LP was effective in decreasing early failure in 44.4% of cases. Radiological regression of hydrocephalic changes was detected in 50% of the cases. Conclusion: ETV is an efficient and safe procedure in the treatment of hydrocephalus in children with Chiari malformation II, particularly when performed as a secondary procedure. Serial LP following the procedure increases the success rate in patients with early failure.
MeSH term(s)	Humans ; Child ; Arnold-Chiari Malformation/complications ; Arnold-Chiari Malformation/diagnostic imaging ; Arnold-Chiari Malformation/surgery ; Ventriculostomy/methods ; Prospective Studies ; Third Ventricle/diagnostic imaging ; Third Ventricle/surgery ; Treatment Outcome ; Neuroendoscopy/methods ; Hydrocephalus/diagnostic imaging ; Hydrocephalus/etiology ; Hydrocephalus/surgery ; Retrospective Studies
Language	English
Publishing date	2023-01-26
Publishing country	Germany
Document type	Journal Article
ZDB-ID	605988-0
ISSN	1433-0350 ; 0302-2803 ; 0256-7040
ISSN (online)	1433-0350
ISSN	0302-2803 ; 0256-7040
DOI	10.1007/s00381-023-05832-4
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Zs.A 2036: Show issues

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Book: Awareness during anesthesia

Ghoneim, Mohamed M.

2001

Author's details	ed. by Mohamed M. Ghoneim
Language	English
Size	XXII, 181 S. : graph. Darst.
Publisher	Butterworth Heinemann
Publishing place	Oxford u.a.
Publishing country	Great Britain
Document type	Book
HBZ-ID	HT012848381
ISBN	0-7506-7201-3 ; 978-0-7506-7201-6
Database	Catalogue ZB MED Medicine, Health

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Article ; Online: Occurrence, distribution, and composition of black sand along the Red Sea, Egypt.

Hamed, Mohamed / El-Aal, Mohamed Abd / Khaled, Mostafa A / Ghoneim, Sobhi M / Saad, Eman / Lee, Jae-Seong / Sayed, Alaa El-Din H

The Science of the total environment

2024 Volume 923, Page(s) 171277

Abstract: Black sand along the Red Sea is often composed of volcanic minerals and heavy minerals. The Red Sea region is known for its unique geological features, and black sand beaches can be found in various areas along its shores. The study presents a ... ...

Abstract	Black sand along the Red Sea is often composed of volcanic minerals and heavy minerals. The Red Sea region is known for its unique geological features, and black sand beaches can be found in various areas along its shores. The study presents a comprehensive semi-quantitative chemical analysis of black sand samples collected from various locations along the red sea, revealing significant variations in their elemental compositions. The main oxides were identified in each sample, determined through X-ray diffraction (XRD) and X-ray fluorescence (XRF) analyses, indicate diverse mineralogical compositions. The spatial distribution of minerals at each site is depicted through mapping. Additionally, Fourier-transform infrared (FTIR) spectra offer information on the functional groups present in the samples, revealing the existence of hydroxyl groups, aliphatic compounds, and adsorbed water molecules. For Qusier-Elsharm Alqbly, Safaga, Marsa Alam, Gabal Alrosass, Hurghada Titanic, Hurghada Elahiaa, Gemsa, and Ras Elbehar samples, the results highlight the presence of various minerals, such as Quartz, Calcite, Titanium Dioxide, Magnetite, Hematite, Aluminum Oxide, Zirconium Dioxide, Chromium (III) Oxide, and others, providing insights into the geological characteristics of each location. The differences in mineral content among the examined sites are linked to the geological and mineralogical makeup of the source rocks upstream and midstream in the basins that discharge into the surveyed regions. So, variations in black sand concentrations among different locations offer insights into the geological and mineralogical diversity of the studied areas along the Red Sea coast. This study addresses the existing knowledge gap by focusing on the preliminary exploration and description of the occurrence, distribution, and composition of black sand along the Red Sea in Egypt. whereas the results provide valuable insights into the geological diversity of black sand deposits in the surveyed areas, underscoring the need for additional research and interpretation of these variations. Therefore, the in-depth examination of mineralogical composition and crystal structures establishes a foundation for future investigations in the field of geology and earth sciences.
Language	English
Publishing date	2024-02-24
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	121506-1
ISSN	1879-1026 ; 0048-9697
ISSN (online)	1879-1026
ISSN	0048-9697
DOI	10.1016/j.scitotenv.2024.171277
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Zs.A 949: Show issues

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Article: Royal Jelly and

Khadrawy, Sally M / Mohamed, Doaa Sh / Hassan, Randa M / Abdelgawad, Mohamed A / Ghoneim, Mohammed M / Alshehri, Sultan / Shaban, Nema S

Foods (Basel, Switzerland)

2023 Volume 12, Issue 6

Abstract: Gibberellic acid (GA3) is a well-known plant growth regulator used in several countries, but its widespread use has negative effects on both animal and human health. The current study assesses the protective effect of royal jelly (RJ) ... ...

Abstract	Gibberellic acid (GA3) is a well-known plant growth regulator used in several countries, but its widespread use has negative effects on both animal and human health. The current study assesses the protective effect of royal jelly (RJ) and
Language	English
Publishing date	2023-03-13
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2704223-6
ISSN	2304-8158
ISSN	2304-8158
DOI	10.3390/foods12061223
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Article ; Online: Imatinib mitigates experimentally-induced ulcerative colitis: Possible contribution of NF-kB/JAK2/STAT3/COX2 signaling pathway.

Shalaby, Mohamed / Abdelaziz, Rania R / Ghoneim, Hamdy A / Suddek, Ghada M

Life sciences

2023 Volume 321, Page(s) 121596

Abstract: Rationale: Ulcerative colitis (UC) is a chronic immune-mediated disease characterized by recurrent inflammation, damage, and alteration of the large intestine's mucosal and submucosal surfaces. The purpose of this research was to evaluate the impact of ... ...

Abstract	Rationale: Ulcerative colitis (UC) is a chronic immune-mediated disease characterized by recurrent inflammation, damage, and alteration of the large intestine's mucosal and submucosal surfaces. The purpose of this research was to evaluate the impact of tyrosine kinase inhibitor (imatinib) on experimentally induced UC in rats via acetic acid (AA). Methods: Male rats were randomly assigned to four groups: control, AA, AA + imatinib (10 mg/kg), and AA + imatinib (20 mg/kg). Imatinib (10 and 20 mg/kg/day) was orally supplied by oral syringe for one week before induction of UC. On the eighth day, Rats received enemas containing a 4 % solution of acetic acid to induce colitis. One day after inducing colitis, rats were euthanized and their colons were subjected to morphological, biochemical, histological, and immunohistochemical analysis. Results: Imatinib pretreatment significantly decreased macroscopic and histological damage scores, decreased disease activity index as well as colon mass index. In addition, imatinib successfully lowered the levels of malondialdehyde (MDA) in colonic tissues and enhanced superoxide dismutase activity (SOD) and glutathione content (GSH). Imatinib also reduced colonic levels of inflammatory interleukins (IL-23, IL-17, IL-6), JAK2 and STAT3. Furthermore, imatinib suppressed nuclear transcription factor kappa B (NF-kB/p65) level, and COX2 expression in colonic tissues. Significance: Imatinib may be a viable therapy option for UC as it halts the interaction network of NF-kB/JAK2/STAT3/COX2 signaling pathway.
MeSH term(s)	Rats ; Male ; Animals ; Colitis, Ulcerative/chemically induced ; Colitis, Ulcerative/drug therapy ; Colitis, Ulcerative/metabolism ; NF-kappa B/metabolism ; Imatinib Mesylate/pharmacology ; Cyclooxygenase 2/metabolism ; Colon/metabolism ; Signal Transduction ; Colitis/pathology ; Glutathione/metabolism ; Acetic Acid ; Janus Kinase 2/metabolism
Chemical Substances	NF-kappa B ; Imatinib Mesylate (8A1O1M485B) ; Cyclooxygenase 2 (EC 1.14.99.1) ; Glutathione (GAN16C9B8O) ; Acetic Acid (Q40Q9N063P) ; Jak2 protein, rat (EC 2.7.10.2) ; Janus Kinase 2 (EC 2.7.10.2)
Language	English
Publishing date	2023-03-20
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	3378-9
ISSN	1879-0631 ; 0024-3205
ISSN (online)	1879-0631
ISSN	0024-3205
DOI	10.1016/j.lfs.2023.121596
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Article: Appraisal of the Accuracy and Reliability of Cone-Beam Computed Tomography and Three-Dimensional Printing for Volumetric Mandibular Condyle Measurements of a Human Condyle.

Elrawdy, Ahmed M / Amer, Mohamed E / Algariah, Ahmed K / Eid, Mohamed H / Abu-Elsaoud, Abdelghafar M / Ghoneim, Mohamed M

Cureus

2023 Volume 15, Issue 10, Page(s) e46746

Abstract: Background This study aims to evaluate the accuracy of volumetric measurements of three-dimensional (3D)-printed human condyles from cone-beam computed tomography (CBCT) in comparison to physical condyles using a water displacement test. Methodology A ... ...

Abstract	Background This study aims to evaluate the accuracy of volumetric measurements of three-dimensional (3D)-printed human condyles from cone-beam computed tomography (CBCT) in comparison to physical condyles using a water displacement test. Methodology A sample of 22 dry condyles was separated from the mandibular body by disc, mounted on a base made of casting wax, and scanned using the SCANORA (Scanora 3DX, Soredex, Finland) CBCT scanner. Subsequently, the projection data were reconstructed with the machine-dedicated OnDemand 3D (Cybermed Co., Seoul, Korea). The Standard Tessellation Language file was prepared for 3D printing using chitubox slicing software v1.9.1. Frozen water-washable gray resin was used for 3D printing. All condyles were printed using the same parameters and the same resin. The volumetric measurements were then performed using a customized modified pycnometer based on water volume and weight displacement. Volumetric measures were performed for both the physical human condyles and the 3D-printed replicas and the measurements were then compared. Results The volume of dry condyles using the water displacement method showed an average (±SD) of 1.925 ± 0.40 cm
Language	English
Publishing date	2023-10-09
Publishing country	United States
Document type	Journal Article
ZDB-ID	2747273-5
ISSN	2168-8184
ISSN	2168-8184
DOI	10.7759/cureus.46746
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Article ; Online: Evaluating the role of polysaccharide extracted from Pleurotus columbinus on cisplatin-induced oxidative renal injury.

Mohamed, Sahar S / Ibrahim, Ghada S / Ghoneim, Mona A M / Hassan, Amal I

Scientific reports

2023 Volume 13, Issue 1, Page(s) 835

Abstract: This research aimed to examine the antioxidant polysaccharide activity (PsPc-3) derived from Pleurotus columbinus (P. columbinus) on oxidative renal injury (ORI) induced by cisplatin (CP). The principal components of crude polysaccharide were assessed. ... ...

Abstract	This research aimed to examine the antioxidant polysaccharide activity (PsPc-3) derived from Pleurotus columbinus (P. columbinus) on oxidative renal injury (ORI) induced by cisplatin (CP). The principal components of crude polysaccharide were assessed. We studied the preventive impact of polysaccharide on cisplatin-induced renal damage in this study. For 21 days, we employed the CP-induced ORI rat model and divided the rats into four groups: control, CP alone, polysaccharide post CP (100 mg/kg) orally, and CP + polysaccharide (pre and post). The chemical characterization of the polysaccharide fraction PsPc-3 stated that protein was not present. PsPc-3 contained 7.2% uronic acid as assessed as 0% sulfate. PsPc-3 hydrolysate structured of Galacturonic:Glucose:Xylose and their molar proportions were 1:4:5, respectively. The average molecular weight (Mw) and molecular mass (Mn) per molecule of PsPc-3 were 5.49 × 10
MeSH term(s)	Rats ; Animals ; Cisplatin/pharmacology ; Kidney/metabolism ; Oxidative Stress ; Pleurotus/metabolism ; Antioxidants/pharmacology ; Antioxidants/metabolism ; Antineoplastic Agents/pharmacology
Chemical Substances	Cisplatin (Q20Q21Q62J) ; Antioxidants ; Antineoplastic Agents
Language	English
Publishing date	2023-01-16
Publishing country	England
Document type	Journal Article
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-022-27081-2
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Article: A Novel Hydroxyapatite/Vitamin B

Belal, Amany / Mahmoud, Rehab / Mohamed, Eman E / Farghali, Ahmed / Abo El-Ela, Fatma I / Gamal, Amr / Halfaya, Fatma Mohamed / Khaled, Esraa / Farahat, Abdelbasset A / Hassan, Ahmed H E / Ghoneim, Mohammed M / Taha, Mohamed / Zaky, Mohamed Y

Pharmaceuticals (Basel, Switzerland)

2023 Volume 16, Issue 4

Abstract: The usage of nanomaterials for rheumatoid arthritis (RA) treatment can improve bioavailability and enable selective targeting. The current study prepares and evaluates the in vivo biological effects of a novel hydroxyapatite/vitamin ... ...

Abstract	The usage of nanomaterials for rheumatoid arthritis (RA) treatment can improve bioavailability and enable selective targeting. The current study prepares and evaluates the in vivo biological effects of a novel hydroxyapatite/vitamin B
Language	English
Publishing date	2023-04-06
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2193542-7
ISSN	1424-8247
ISSN	1424-8247
DOI	10.3390/ph16040551
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