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  1. Article ; Online: Light-inducible T cell engagers trigger, tune, and shape the activation of primary T cells.

    Jaeger, Morgane / Anastasio, Amandine / Chamy, Léa / Brustlein, Sophie / Vincentelli, Renaud / Durbesson, Fabien / Gigan, Julien / Thépaut, Morgane / Char, Rémy / Boussand, Maud / Lechelon, Mathias / Argüello, Rafael J / Marguet, Didier / He, Hai-Tao / Lasserre, Rémi

    Proceedings of the National Academy of Sciences of the United States of America

    2023  Volume 120, Issue 39, Page(s) e2302500120

    Abstract: To mount appropriate responses, T cells integrate complex sequences of receptor stimuli perceived during transient interactions with antigen-presenting cells. Although it has been hypothesized that the dynamics of these interactions influence the outcome ...

    Abstract To mount appropriate responses, T cells integrate complex sequences of receptor stimuli perceived during transient interactions with antigen-presenting cells. Although it has been hypothesized that the dynamics of these interactions influence the outcome of T cell activation, methodological limitations have hindered its formal demonstration. Here, we have engineered the Light-inducible T cell engager (LiTE) system, a recombinant optogenetics-based molecular tool targeting the T cell receptor (TCR). The LiTE system constitutes a reversible molecular switch displaying exquisite reactivity. As proof of concept, we dissect how specific temporal patterns of TCR stimulation shape T cell activation. We established that CD4
    MeSH term(s) CD8-Positive T-Lymphocytes ; Antigen-Presenting Cells ; Cytokines ; Epithelial Cells ; Lymphocyte Activation
    Chemical Substances Cytokines
    Language English
    Publishing date 2023-09-18
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 209104-5
    ISSN 1091-6490 ; 0027-8424
    ISSN (online) 1091-6490
    ISSN 0027-8424
    DOI 10.1073/pnas.2302500120
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    Zs.A 1148: Show issues Location:
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  2. Article ; Online: Experimental evidence for long-distance electrodynamic intermolecular forces.

    Lechelon, Mathias / Meriguet, Yoann / Gori, Matteo / Ruffenach, Sandra / Nardecchia, Ilaria / Floriani, Elena / Coquillat, Dominique / Teppe, Frédéric / Mailfert, Sébastien / Marguet, Didier / Ferrier, Pierre / Varani, Luca / Sturgis, James / Torres, Jeremie / Pettini, Marco

    Science advances

    2022  Volume 8, Issue 7, Page(s) eabl5855

    Abstract: Both classical and quantum electrodynamics predict the existence of dipole-dipole long-range electrodynamic intermolecular forces; however, these have never been hitherto experimentally observed. The discovery of completely new and unanticipated forces ... ...

    Abstract Both classical and quantum electrodynamics predict the existence of dipole-dipole long-range electrodynamic intermolecular forces; however, these have never been hitherto experimentally observed. The discovery of completely new and unanticipated forces acting between biomolecules could have considerable impact on our understanding of the dynamics and functioning of the molecular machines at work in living organisms. Here, using two independent experiments, on the basis of different physical effects detected by fluorescence correlation spectroscopy and terahertz spectroscopy, respectively, we demonstrate experimentally the activation of resonant electrodynamic intermolecular forces. This is an unprecedented experimental proof of principle of a physical phenomenon that, having been observed for biomacromolecules and with long-range action (up to 1000 Å), could be of importance for biology. In addition to thermal fluctuations that drive molecular motion randomly, these resonant (and thus selective) electrodynamic forces may contribute to molecular encounters in the crowded cellular space.
    Language English
    Publishing date 2022-02-16
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2810933-8
    ISSN 2375-2548 ; 2375-2548
    ISSN (online) 2375-2548
    ISSN 2375-2548
    DOI 10.1126/sciadv.abl5855
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  3. Article ; Online: Reply to: "Validating subcellular thermal changes revealed by fluorescent thermosensors" and "The 10(5) gap issue between calculation and measurement in single-cell thermometry".

    Baffou, Guillaume / Rigneault, Hervé / Marguet, Didier / Jullien, Ludovic

    Nature methods

    2015  Volume 12, Issue 9, Page(s) 803

    MeSH term(s) Algorithms ; Animals ; Artifacts ; Cell Physiological Phenomena ; Humans ; Models, Biological ; Thermography/methods
    Language English
    Publishing date 2015-09
    Publishing country United States
    Document type Comment ; Letter
    ZDB-ID 2169522-2
    ISSN 1548-7105 ; 1548-7091
    ISSN (online) 1548-7105
    ISSN 1548-7091
    DOI 10.1038/nmeth.3552
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    Zs.A 6022: Show issues Location:
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  4. Article ; Online: De novo variants in SP9 cause a novel form of interneuronopathy characterized by intellectual disability, autism spectrum disorder, and epilepsy with variable expressivity.

    Tessarech, Marine / Friocourt, Gaëlle / Marguet, Florent / Lecointre, Maryline / Le Mao, Morgane / Díaz, Rodrigo Muñoz / Mignot, Cyril / Keren, Boris / Héron, Bénédicte / De Bie, Charlotte / Van Gassen, Koen / Loisel, Didier / Delorme, Benoit / Syrbe, Steffen / Klabunde-Cherwon, Annick / Jamra, Rami Abou / Wegler, Meret / Callewaert, Bert / Dheedene, Annelies /
    Zidane-Marinnes, Merzouka / Guichet, Agnès / Bris, Céline / Van Bogaert, Patrick / Biquard, Florence / Lenaers, Guy / Marcorelles, Pascale / Ferec, Claude / Gonzalez, Bruno / Procaccio, Vincent / Vitobello, Antonio / Bonneau, Dominique / Laquerriere, Annie / Khiati, Salim / Colin, Estelle

    Genetics in medicine : official journal of the American College of Medical Genetics

    2024  Volume 26, Issue 5, Page(s) 101087

    Abstract: Purpose: Interneuronopathies are a group of neurodevelopmental disorders characterized by deficient migration and differentiation of gamma-aminobutyric acidergic interneurons resulting in a broad clinical spectrum, including autism spectrum disorders, ... ...

    Abstract Purpose: Interneuronopathies are a group of neurodevelopmental disorders characterized by deficient migration and differentiation of gamma-aminobutyric acidergic interneurons resulting in a broad clinical spectrum, including autism spectrum disorders, early-onset epileptic encephalopathy, intellectual disability, and schizophrenic disorders. SP9 is a transcription factor belonging to the Krüppel-like factor and specificity protein family, the members of which harbor highly conserved DNA-binding domains. SP9 plays a central role in interneuron development and tangential migration, but it has not yet been implicated in a human neurodevelopmental disorder.
    Methods: Cases with SP9 variants were collected through international data-sharing networks. To address the specific impact of SP9 variants, in silico and in vitro assays were carried out.
    Results: De novo heterozygous variants in SP9 cause a novel form of interneuronopathy. SP9 missense variants affecting the glutamate 378 amino acid result in severe epileptic encephalopathy because of hypomorphic and neomorphic DNA-binding effects, whereas SP9 loss-of-function variants result in a milder phenotype with epilepsy, developmental delay, and autism spectrum disorder.
    Conclusion: De novo heterozygous SP9 variants are responsible for a neurodevelopmental disease. Interestingly, variants located in conserved DNA-binding domains of KLF/SP family transcription factors may lead to neomorphic DNA-binding functions resulting in a combination of loss- and gain-of-function effects.
    Language English
    Publishing date 2024-01-27
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1455352-1
    ISSN 1530-0366 ; 1098-3600
    ISSN (online) 1530-0366
    ISSN 1098-3600
    DOI 10.1016/j.gim.2024.101087
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    Zs.A 5059: Show issues Location:
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  5. Article: Microangiopathy in primary familial brain calcification: Evidence from skin biopsies.

    Nicolas, Gaël / Marguet, Florent / Laquerrière, Annie / Mendes de Oliveira, João Ricardo / Hannequin, Didier

    Neurology. Genetics

    2017  Volume 3, Issue 2, Page(s) e134

    Language English
    Publishing date 2017-02-08
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2818607-2
    ISSN 2376-7839
    ISSN 2376-7839
    DOI 10.1212/NXG.0000000000000134
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells.

    Mailfert, Sebastien / Wojtowicz, Karolina / Brustlein, Sophie / Blaszczak, Ewa / Bertaux, Nicolas / Łukaszewicz, Marcin / Marguet, Didier / Trombik, Tomasz

    Journal of visualized experiments : JoVE

    2020  , Issue 165

    Abstract: Dynamic biological processes in living cells, including those associated with plasma membrane organization, occur on various spatial and temporal scales, ranging from nanometers to micrometers and microseconds to minutes, respectively. Such a broad range ...

    Abstract Dynamic biological processes in living cells, including those associated with plasma membrane organization, occur on various spatial and temporal scales, ranging from nanometers to micrometers and microseconds to minutes, respectively. Such a broad range of biological processes challenges conventional microscopy approaches. Here, we detail the procedure for implementing spot variation Fluorescence Correlation Spectroscopy (svFCS) measurements using a classical fluorescence microscope that has been customized. The protocol includes a specific performance check of the svFCS setup and the guidelines for molecular diffusion measurements by svFCS on the plasma membrane of living cells under physiological conditions. Additionally, we provide a procedure for disrupting plasma membrane raft nanodomains by cholesterol oxidase treatment and demonstrate how these changes in the lateral organization of the plasma membrane might be revealed by svFCS analysis. In conclusion, this fluorescence-based method can provide unprecedented details on the lateral organization of the plasma membrane with the appropriate spatial and temporal resolution.
    MeSH term(s) Animals ; COS Cells ; Calibration ; Cell Membrane/metabolism ; Cell Survival ; Chlorocebus aethiops ; Cholesterol/metabolism ; Diffusion ; Green Fluorescent Proteins/metabolism ; Membrane Microdomains/chemistry ; Membrane Microdomains/metabolism ; Spectrometry, Fluorescence
    Chemical Substances Green Fluorescent Proteins (147336-22-9) ; Cholesterol (97C5T2UQ7J)
    Language English
    Publishing date 2020-11-12
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Video-Audio Media
    ZDB-ID 2259946-0
    ISSN 1940-087X ; 1940-087X
    ISSN (online) 1940-087X
    ISSN 1940-087X
    DOI 10.3791/61823
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  7. Article ; Online: La(s)t but not least.

    Malissen, Bernard / Marguet, Didier

    Nature immunology

    2011  Volume 12, Issue 7, Page(s) 592–593

    MeSH term(s) Adaptor Proteins, Signal Transducing/immunology ; Animals ; Membrane Proteins/immunology ; Mice ; Phosphoproteins/immunology ; Protein-Tyrosine Kinases/immunology ; Receptors, Antigen, T-Cell/immunology ; Signal Transduction/immunology ; T-Lymphocytes/immunology
    Chemical Substances Adaptor Proteins, Signal Transducing ; Lat protein, mouse ; Membrane Proteins ; Phosphoproteins ; Receptors, Antigen, T-Cell ; Protein-Tyrosine Kinases (EC 2.7.10.1)
    Language English
    Publishing date 2011-06-20
    Publishing country United States
    Document type Comment ; News
    ZDB-ID 2016987-5
    ISSN 1529-2916 ; 1529-2908
    ISSN (online) 1529-2916
    ISSN 1529-2908
    DOI 10.1038/ni.2054
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    Zs.A 5294: Show issues Location:
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    Zs.MG 42: Show issues

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  8. Article ; Online: Detecting nanodomains in living cell membrane by fluorescence correlation spectroscopy.

    He, Hai-Tao / Marguet, Didier

    Annual review of physical chemistry

    2011  Volume 62, Page(s) 417–436

    Abstract: Cell membranes actively participate in numerous cellular functions. Inasmuch as bioactivities of cell membranes are known to depend crucially on their lateral organization, much effort has been focused on deciphering this organization on different length ...

    Abstract Cell membranes actively participate in numerous cellular functions. Inasmuch as bioactivities of cell membranes are known to depend crucially on their lateral organization, much effort has been focused on deciphering this organization on different length scales. Within this context, the concept of lipid rafts has been intensively discussed over recent years. In line with its ability to measure diffusion parameters with great precision, fluorescence correlation spectroscopy (FCS) measurements have been made in association with innovative experimental strategies to monitor modes of molecular lateral diffusion within the plasma membrane of living cells. These investigations have allowed significant progress in the characterization of the cell membrane lateral organization at the suboptical level and have provided compelling evidence for the in vivo existence of raft nanodomains. We review these FCS-based studies and the characteristic structural features of raft nanodomains. We also discuss the findings in regards to the current view of lipid rafts as a general membrane-organizing principle.
    MeSH term(s) Cell Membrane/chemistry ; Cell Membrane/metabolism ; Diffusion ; Fluorescence ; Lipids/chemistry ; Membrane Microdomains/chemistry ; Membrane Microdomains/metabolism ; Spectrometry, Fluorescence/methods
    Chemical Substances Lipids
    Language English
    Publishing date 2011
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 1470474-2
    ISSN 1545-1593 ; 0066-426X
    ISSN (online) 1545-1593
    ISSN 0066-426X
    DOI 10.1146/annurev-physchem-032210-103402
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  9. Article ; Online: A critique of methods for temperature imaging in single cells.

    Baffou, Guillaume / Rigneault, Hervé / Marguet, Didier / Jullien, Ludovic

    Nature methods

    2014  Volume 11, Issue 9, Page(s) 899–901

    Abstract: We argue that standard thermodynamic considerations and scaling laws show that a single cell cannot substantially raise its temperature by endogenous thermogenesis. This statement seriously questions the interpretations of recent work reporting ... ...

    Abstract We argue that standard thermodynamic considerations and scaling laws show that a single cell cannot substantially raise its temperature by endogenous thermogenesis. This statement seriously questions the interpretations of recent work reporting temperature heterogeneities measured in single living cells.
    MeSH term(s) Algorithms ; Animals ; Artifacts ; Cell Physiological Phenomena ; Computer Simulation ; Humans ; Models, Biological ; Reproducibility of Results ; Sensitivity and Specificity ; Thermography/methods
    Language English
    Publishing date 2014-08-28
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2169522-2
    ISSN 1548-7105 ; 1548-7091
    ISSN (online) 1548-7105
    ISSN 1548-7091
    DOI 10.1038/nmeth.3073
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  10. Article: Spot variation fluorescence correlation spectroscopy for analysis of molecular diffusion at the plasma membrane of living cells

    Mailfert, Sebastien / Wojtowicz, Karolina / Brustlein, Sophie / Blaszczak, Ewa / Bertaux, Nicolas / Łukaszewicz, Marcin / Marguet, Didier / Trombik, Tomasz

    Journal of visualized experiments. 2020 Nov. 12, , no. 165

    2020  

    Abstract: Dynamic biological processes in living cells, including those associated with plasma membrane organization, occur on various spatial and temporal scales, ranging from nanometers to micrometers and microseconds to minutes, respectively. Such a broad range ...

    Abstract Dynamic biological processes in living cells, including those associated with plasma membrane organization, occur on various spatial and temporal scales, ranging from nanometers to micrometers and microseconds to minutes, respectively. Such a broad range of biological processes challenges conventional microscopy approaches. Here, we detail the procedure for implementing spot variation Fluorescence Correlation Spectroscopy (svFCS) measurements using a classical fluorescence microscope that has been customized. The protocol includes a specific performance check of the svFCS setup and the guidelines for molecular diffusion measurements by svFCS on the plasma membrane of living cells under physiological conditions. Additionally, we provide a procedure for disrupting plasma membrane raft nanodomains by cholesterol oxidase treatment and demonstrate how these changes in the lateral organization of the plasma membrane might be revealed by svFCS analysis. In conclusion, this fluorescence-based method can provide unprecedented details on the lateral organization of the plasma membrane with the appropriate spatial and temporal resolution.
    Keywords cholesterol oxidase ; fluorescence correlation spectroscopy ; fluorescence microscopes ; microscopy ; plasma membrane
    Language English
    Dates of publication 2020-1112
    Size p. e61823.
    Publishing place Journal of Visualized Experiments
    Document type Article
    ZDB-ID 2259946-0
    ISSN 1940-087X
    ISSN 1940-087X
    DOI 10.3791/61823
    Database NAL-Catalogue (AGRICOLA)

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