Artikel ; Online: Insulin oversecretion in MSG-obese rats is related to alterations in cholinergic muscarinic receptor subtypes in pancreatic islets.
2014 Band 33, Heft 4, Seite(n) 1075–1086
Abstract: Background/aims: Impaired pancreatic beta cell function and insulin secretion/action are a link between obesity and type 2 diabetes, which are worldwide public health burdens. We aimed to characterize the muscarinic acetylcholine receptor (mAChR) M1-M4 ... ...
| Abstract | Background/aims: Impaired pancreatic beta cell function and insulin secretion/action are a link between obesity and type 2 diabetes, which are worldwide public health burdens. We aimed to characterize the muscarinic acetylcholine receptor (mAChR) M1-M4 subtypes in isolated pancreatic islets from pre-diabetic obese rats that had been treated neonatally with monosodium L-glutamate (MSG). Methods: At 90 days of age, both the MSG and the control groups underwent biometric and biochemical evaluation. Anti-muscarinic drugs were used to study mAChR function either in vivo or in vitro. Results: The results demonstrated that atropine treatment reduced insulin secretion in the MSG-treated and control groups, whereas treatment with an M2mAChR-selective antagonist increased secretion. Moreover, the insulinostatic effect of an M3mAChR-selective antagonist was significantly higher in the MSG-treated group. M1mAChR and M3mAChR expression was increased in the MSG-obese group by 55% and 73%, respectively. In contrast, M2mAChR expression decreased by 25% in the MSG group, whereas M4mAChR expression was unchanged. Conclusions: Functional changes in and altered content of the mAChR (M1-M4) subtypes are pivotal to the demand for high pancreatic beta cell insulin secretion in MSG-obese rats, which is directly associated with vagal hyperactivity and peripheral insulin resistance. |
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| Mesh-Begriff(e) | Animals ; Blood Glucose/analysis ; Glucose Tolerance Test ; Insulin/metabolism ; Insulin Secretion ; Islets of Langerhans/drug effects ; Islets of Langerhans/metabolism ; Male ; Muscarinic Agonists/pharmacology ; Muscarinic Antagonists/pharmacology ; Obesity/metabolism ; Obesity/pathology ; Rats ; Rats, Wistar ; Receptor, Muscarinic M1/metabolism ; Receptor, Muscarinic M2/metabolism ; Receptor, Muscarinic M3/metabolism ; Receptor, Muscarinic M4/metabolism ; Receptors, Muscarinic/chemistry ; Receptors, Muscarinic/metabolism ; Sodium Glutamate/pharmacology |
| Chemische Substanzen | Blood Glucose ; Insulin ; Muscarinic Agonists ; Muscarinic Antagonists ; Receptor, Muscarinic M1 ; Receptor, Muscarinic M2 ; Receptor, Muscarinic M3 ; Receptor, Muscarinic M4 ; Receptors, Muscarinic ; Sodium Glutamate (W81N5U6R6U) |
| Sprache | Englisch |
| Erscheinungsdatum | 2014-04-09 |
| Erscheinungsland | Germany |
| Dokumenttyp | Journal Article ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 1067572-3 |
| ISSN | 1421-9778 ; 1015-8987 |
| ISSN (online) | 1421-9778 |
| ISSN | 1015-8987 |
| DOI | 10.1159/000358677 |
| Datenquelle | MEDical Literature Analysis and Retrieval System OnLINE |
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