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  1. Article: Effect of dissolved metabolites of the dinoflagellate

    Flores-Chavarria, A M / Rodríguez-Jaramillo, Carmen / Band-Schmidt, Christine J / Hernández-Sandoval, Francisco E / Núñez-Vázquez, Erick / Bustillos-Guzmán, José J

    Heliyon

    2023  Volume 9, Issue 6, Page(s) e17018

    Abstract: Harmful algae blooms (HABs) are a conspicuous phenomenon that affect the coastal zone worldwide. Aquaculture industry zones are not excluded from being affected by HAB that cause organism mortality and jeopardize their innocuity due to the contamination ... ...

    Abstract Harmful algae blooms (HABs) are a conspicuous phenomenon that affect the coastal zone worldwide. Aquaculture industry zones are not excluded from being affected by HAB that cause organism mortality and jeopardize their innocuity due to the contamination by phytotoxins with the concomitant economic losses. Direct ingestion of metabolites from HAB species or organisms contaminated with phycotoxins together with dermal absorption of dissolved metabolites (DM), including toxins, are the two main routes of poisoning. From these poisoning routes, the effect of DM, particularly paralytic shellfish toxins (PST), has been relatively understudied. This intoxication route can be conspicuous and could be involved in many significant mortalities of cultivated marine organisms. In this study, white shrimp juveniles (2.1 g wet weight) of
    Language English
    Publishing date 2023-06-05
    Publishing country England
    Document type Journal Article
    ZDB-ID 2835763-2
    ISSN 2405-8440
    ISSN 2405-8440
    DOI 10.1016/j.heliyon.2023.e17018
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Metabolomic Insights of Biosurfactant Activity from

    Sánchez-Lozano, Ilse / Muñoz-Cruz, Luz Clarita / Hellio, Claire / Band-Schmidt, Christine J / Cruz-Narváez, Yair / Becerra-Martínez, Elvia / Hernández-Guerrero, Claudia J

    International journal of molecular sciences

    2023  Volume 24, Issue 4

    Abstract: In marine environments, biofilm can cause negative impacts, including the biofouling process. In the search for new non-toxic formulations that inhibit biofilm, biosurfactants (BS) produced by the ... ...

    Abstract In marine environments, biofilm can cause negative impacts, including the biofouling process. In the search for new non-toxic formulations that inhibit biofilm, biosurfactants (BS) produced by the genus
    MeSH term(s) Biofouling ; Pseudomonas stutzeri ; Plankton ; NADP/metabolism ; Trehalose/metabolism ; Biofilms ; Bacillus
    Chemical Substances NADP (53-59-8) ; Trehalose (B8WCK70T7I)
    Language English
    Publishing date 2023-02-20
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms24044249
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Stingray Envenomation: Consequences of an Embedded Spine.

    Mora-Zamacona, Pablo / Águila-Ramírez, Ruth N / Muñoz-Ochoa, Mauricio / Pérez-Palafox, Xchel A / Sepúlveda De La Rosa, Yanet / Band-Schmidt, Christine J / Cruz-Escalona, Víctor H

    Cureus

    2023  Volume 15, Issue 5, Page(s) e38885

    Abstract: Rays and skates are fish with flattened, pancake-shaped bodies that frequent shallow water, where they often lie hidden under the sand. Some of the batoid species are characterized by a stinger with serrated edges, which is covered by a tegument made up ... ...

    Abstract Rays and skates are fish with flattened, pancake-shaped bodies that frequent shallow water, where they often lie hidden under the sand. Some of the batoid species are characterized by a stinger with serrated edges, which is covered by a tegument made up of specialized cells that secrete toxins and enzymes with proteolytic activity. Stingray injuries to humans are common in warm coastal regions. In this report, we present a case of an injury due to the insertion of a barb from a Pacific cownose ray, Rhinoptera steindachneri
    Language English
    Publishing date 2023-05-11
    Publishing country United States
    Document type Case Reports
    ZDB-ID 2747273-5
    ISSN 2168-8184
    ISSN 2168-8184
    DOI 10.7759/cureus.38885
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Relationship between paralytic shellfish toxin content and sxtA gene copy number in different growth phases of Gymnodinium catenatum (Dinophyceae).

    Mendoza-Flores, Armando / Leyva-Valencia, Ignacio / Hernández-Sandoval, Francisco E / Galindo-Sánchez, Clara E / Band-Schmidt, Christine J / Bustillos-Guzmán, José J

    Toxicon : official journal of the International Society on Toxinology

    2021  Volume 199, Page(s) 68–71

    Abstract: Paralytic shellfish toxin (PST) content in the dinoflagellate Gymnodinium catenatum changes with culture age, with a higher toxin concentration in the logarithmic phase that decreases when the culture ages. The gene copy number (GCN) of domains sxtA1 and ...

    Abstract Paralytic shellfish toxin (PST) content in the dinoflagellate Gymnodinium catenatum changes with culture age, with a higher toxin concentration in the logarithmic phase that decreases when the culture ages. The gene copy number (GCN) of domains sxtA1 and sxtA4 was higher in the lag and stationary phase, and lag phase, respectively. No relationship was found between the GCN of the domains sxtA4 and sxtA1 with the PST content in G. catenatum.
    MeSH term(s) Dinoflagellida/genetics ; Gene Dosage ; Humans ; Shellfish ; Shellfish Poisoning ; Toxins, Biological
    Chemical Substances Toxins, Biological
    Language English
    Publishing date 2021-06-01
    Publishing country England
    Document type Journal Article
    ZDB-ID 204479-1
    ISSN 1879-3150 ; 0041-0101
    ISSN (online) 1879-3150
    ISSN 0041-0101
    DOI 10.1016/j.toxicon.2021.05.014
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Effect of Different N:P Ratios on the Growth, Toxicity, and Toxin Profile of

    Hernández-Sandoval, Francisco E / Bustillos-Guzmán, José J / Band-Schmidt, Christine J / Núñez-Vázquez, Erick J / López-Cortés, David J / Fernández-Herrera, Leyberth J / Poot-Delgado, Carlos A / Moreno-Legorreta, Manuel

    Toxins

    2022  Volume 14, Issue 7

    Abstract: The harmful ... ...

    Abstract The harmful microalgae
    MeSH term(s) Animals ; Chromatography, High Pressure Liquid ; Dinoflagellida ; Mammals ; Saxitoxin/analysis ; Shellfish Poisoning ; Toxins, Biological
    Chemical Substances Toxins, Biological ; Saxitoxin (35523-89-8)
    Language English
    Publishing date 2022-07-18
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2518395-3
    ISSN 2072-6651 ; 2072-6651
    ISSN (online) 2072-6651
    ISSN 2072-6651
    DOI 10.3390/toxins14070501
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Effect of dissolved metabolites of the dinoflagellate Gymnodinium catenatum (Graham, 1943) on the white shrimp Litopenaeus vannamei (Boone, 1931)

    A.M. Flores-Chavarria / Carmen Rodríguez-Jaramillo / Christine J. Band-Schmidt / Francisco E. Hernández-Sandoval / Erick Núñez-Vázquez / José J. Bustillos-Guzmán

    Heliyon, Vol 9, Iss 6, Pp e17018- (2023)

    A histological study

    2023  

    Abstract: Harmful algae blooms (HABs) are a conspicuous phenomenon that affect the coastal zone worldwide. Aquaculture industry zones are not excluded from being affected by HAB that cause organism mortality and jeopardize their innocuity due to the contamination ... ...

    Abstract Harmful algae blooms (HABs) are a conspicuous phenomenon that affect the coastal zone worldwide. Aquaculture industry zones are not excluded from being affected by HAB that cause organism mortality and jeopardize their innocuity due to the contamination by phytotoxins with the concomitant economic losses. Direct ingestion of metabolites from HAB species or organisms contaminated with phycotoxins together with dermal absorption of dissolved metabolites (DM), including toxins, are the two main routes of poisoning. From these poisoning routes, the effect of DM, particularly paralytic shellfish toxins (PST), has been relatively understudied. This intoxication route can be conspicuous and could be involved in many significant mortalities of cultivated marine organisms. In this study, white shrimp juveniles (2.1 g wet weight) of Litopenaeus vannamei were exposed to extracts of 104, 105 and 106 cells/L of the dinoflagellate Gymnodinium catenatum, a PST producer. The experiment ended after 17 h of exposure when shrimps exposed to 106 cells/L extract started to die and the rest of the shrimps, from this and other treatments, did not respond to gentle physical stimulus and their swimming activity was low and erratic. Toxin concentrations were determined using high performance liquid chromatography while qualitative and quantitative histological damages were assessed on the tissues. In general, most toxins were accumulated in the hepatopancreas where more than 90% were found. Other tissues such as intestine, muscle, and gills contained less than 10% of toxins. Compared to the control, the main significative tissue damages were, loss of up to 80% of the nerve cord, 40% of the muscle coverage area, and reduction of the gill lamella width. Also, atrophy in hepatopancreas was observed, manifested by a decrease in the height of B cells, lumen degeneration and thinning of tubules. Some damages were more evident when shrimps were exposed to higher concentrated extracts of G. catenatum, however, not all damages were progressive ...
    Keywords Dissolved PST toxins ; Shrimp ; Gymnodinium catenatum ; Quantitative histopathology ; Science (General) ; Q1-390 ; Social sciences (General) ; H1-99
    Language English
    Publishing date 2023-06-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  7. Article: Analysis of concentration-dependent effects of copper and PCB on different Chattonella spp. microalgae (raphidophyceae) cultivated in artificial seawater medium.

    Niestroy, Jeanette / Martínez, Alfonso Bárbara / Band-Schmidt, Christine J

    EXCLI journal

    2014  Volume 13, Page(s) 197–211

    Abstract: In the present study, the effect on the chlorophyll a and the total protein content as well as the Chattonella spp. cell viability were examined after concentration-dependent exposure to CuCl2 and Aroclor 1242. The comparison between various raphidophyte ...

    Abstract In the present study, the effect on the chlorophyll a and the total protein content as well as the Chattonella spp. cell viability were examined after concentration-dependent exposure to CuCl2 and Aroclor 1242. The comparison between various raphidophyte strains provides an insight into the different susceptibilities to contaminants of Chattonella subsalsa (CSNAV-1), C. marina var. marina (CMCV-1) and C. marina var. ovata (COPV-2). The microalgae were cultivated in artificial seawater medium. Exponentially growing microalgae (8-10 days in culture) were used for exposure experiments. We observed in all three raphidophyte species cytotoxicity-mediated modifications beginning at concentrations of 150 and 200 µM of the heavy metal copper after 24 hours exposure. But interestingly, the three strains exhibited only slight differences in their susceptibility to CuCl2. C. subsalsa and C. marina var. marina cells were first affected at the chlorophyll a level and in cell viability. The total protein amount was reduced significantly only after exposure to 300 µM of CuCl2. However, C. marina var. ovata microalgae showed similar reduction curves for all three analysed cytotoxicity endpoints after heavy metal exposure. On the other hand, after Aroclor 1242 incubation the cytotoxic modification pattern indicated clearly the different susceptibilities of the three raphidophyte strains. C. subsalsa cells noticeably exhibited a decrease in the analysed pigment amount (30-20 % compared to that of the control) already after 0.007 mg/L PCB exposure. In contrast, cell viability and total protein content were slightly reduced and fell below the 50 % threshold after 0.7 and 3.3 mg/L of Aroclor 1242, respectively. Interestingly, C. marina var. ovata showed almost no cytotoxic modification caused by the PCB mixture. Only the concentration of 0.7 mg/L Aroclor 1242 clearly affected the cell viability. As opposed to that we observed a concentration-dependent decrease of cell viability and chlorophyll a amount in CMCV-1 microalgae. These observations confirmed that the susceptibility of the raphidophytes strains CSNAV-1, CMCV-1 and COPV-2 is contaminant-dependent. We showed differences even between two variants of Chattonella (Chattonella marina var. marina and C. marina var. ovata). Furthermore, we were able to show the different mode of action of two common pollutants by simple cytotoxic parameters like total protein and chlorophyll a content as well as by cell counting analysis.
    Language English
    Publishing date 2014-02-28
    Publishing country Germany
    Document type Journal Article
    ISSN 1611-2156
    ISSN 1611-2156
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Lipophilic Toxins in Wild Bivalves from the Southern Gulf of California, Mexico.

    Leyva-Valencia, Ignacio / Hernández-Castro, Jesús Ernestina / Band-Schmidt, Christine J / Turner, Andrew D / O'Neill, Alison / Núñez-Vázquez, Erick J / López-Cortés, David J / Bustillos-Guzmán, José J / Hernández-Sandoval, Francisco E

    Marine drugs

    2021  Volume 19, Issue 2

    Abstract: Most of the shellfish fisheries of Mexico occur in the Gulf of California. In this region, known for its high primary productivity, blooms of diatoms and dinoflagellates are common, occurring mainly during upwelling events. Dinoflagellates that produce ... ...

    Abstract Most of the shellfish fisheries of Mexico occur in the Gulf of California. In this region, known for its high primary productivity, blooms of diatoms and dinoflagellates are common, occurring mainly during upwelling events. Dinoflagellates that produce lipophilic toxins are present, where some outbreaks related to okadaic acid and dinophisystoxins have been recorded. From January 2015 to November 2017 samples of three species of wild bivalve mollusks were collected monthly in five sites in the southern region of Bahía de La Paz. Pooled tissue extracts were analyzed using LC-MS/MS to detect lipophilic toxins. Eighteen analogs of seven toxin groups, including cyclic imines were identified, fortunately individual toxins did not exceed regulatory levels and also the total toxin concentration for each bivalve species was lower than the maximum permitted level for human consumption. Interspecific differences in toxin number and concentration were observed in three species of bivalves even when the samples were collected at the same site. Okadaic acid was detected in low concentrations, while yessotoxins and gymnodimines had the highest concentrations in bivalve tissues. Although in low quantities, the presence of cyclic imines and other lipophilic toxins in bivalves from the southern Gulf of California was constant.
    MeSH term(s) Animals ; Bivalvia/metabolism ; Heterocyclic Compounds, 3-Ring/analysis ; Hydrocarbons, Cyclic/analysis ; Imines/analysis ; Marine Toxins/analysis ; Marine Toxins/chemistry ; Okadaic Acid/analysis ; Oxocins/analysis ; Solubility
    Chemical Substances Heterocyclic Compounds, 3-Ring ; Hydrocarbons, Cyclic ; Imines ; Marine Toxins ; Oxocins ; Okadaic Acid (1W21G5Q4N2) ; gymnodimine (7TV3J97IT8) ; yessotoxin (P6M9FM2L2G)
    Language English
    Publishing date 2021-02-09
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2175190-0
    ISSN 1660-3397 ; 1660-3397
    ISSN (online) 1660-3397
    ISSN 1660-3397
    DOI 10.3390/md19020099
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Effect of Different N:P Ratios on the Growth, Toxicity, and Toxin Profile of Gymnodinium catenatum (Dinophyceae) Strains from the Gulf of California

    Francisco E. Hernández-Sandoval / José J. Bustillos-Guzmán / Christine J. Band-Schmidt / Erick J. Núñez-Vázquez / David J. López-Cortés / Leyberth J. Fernández-Herrera / Carlos A. Poot-Delgado / Manuel Moreno-Legorreta

    Toxins, Vol 14, Iss 501, p

    2022  Volume 501

    Abstract: The harmful microalgae Gymnodinium catenatum is a unique naked dinoflagellate that produces paralytic shellfish poisoning toxins (PSTs). This species is common along the coasts of the Mexican Pacific and is responsible for paralytic shellfish poisoning, ... ...

    Abstract The harmful microalgae Gymnodinium catenatum is a unique naked dinoflagellate that produces paralytic shellfish poisoning toxins (PSTs). This species is common along the coasts of the Mexican Pacific and is responsible for paralytic shellfish poisoning, which has resulted in notable financial losses in both fisheries and aquaculture. In the Gulf of California, G. catenatum has been related to mass mortality events in fish, shrimp, seabirds, and marine mammals. In this study, the growth, toxin profiles, and toxin content of four G. catenatum strains isolated from Bahía de La Paz (BAPAZ) and Bahía de Mazatlán (BAMAZ) were evaluated with different N:P ratios, keeping the phosphorus concentration constant. All strains were cultivated in semi-continuous cultures (200 mL, 21.0 °C, 120 µmol photon m −2 s −1 , and a 12:12 h light-dark cycle) with f/2 + Se medium using N:P ratios of: 4:1, 8:1, 16:1, 32:1, and 64:1. Paralytic toxins were analyzed by HPLC with fluorescence detection. Maximum cellular abundance and growth were obtained at an N:P ratio of 64:1 (3188 cells mL −1 and 0.34 div day −1 ) with the BAMAZ and BAPAZ strains. A total of ten saxitoxin analogs dominated by N-sulfocarbamoyl (60–90 mol%), decarbamoyl (10–20 mol%), and carbamoyl (5–10 mol%) toxins were detected. The different N:P ratios did not cause significant changes in the PST content or toxin profiles of the strains from both bays, although they did affect cell abundance.
    Keywords Gymnodinium catenatum ; paralytic toxins ; semi-continuous culture ; toxin profile ; N:P ratio ; Gulf of California ; Medicine ; R
    Subject code 333
    Language English
    Publishing date 2022-07-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  10. Article: Relationship between paralytic shellfish toxin content and sxtA gene copy number in different growth phases of Gymnodinium catenatum (Dinophyceae)

    Mendoza-Flores, Armando / Leyva-Valencia, Ignacio / Hernández-Sandoval, Francisco E / Galindo-Sánchez, Clara E / Band-Schmidt, Christine J / Bustillos-Guzmán, José J

    Toxicon. 2021 Aug., v. 199

    2021  

    Abstract: Paralytic shellfish toxin (PST) content in the dinoflagellate Gymnodinium catenatum changes with culture age, with a higher toxin concentration in the logarithmic phase that decreases when the culture ages. The gene copy number (GCN) of domains sxtA1 and ...

    Abstract Paralytic shellfish toxin (PST) content in the dinoflagellate Gymnodinium catenatum changes with culture age, with a higher toxin concentration in the logarithmic phase that decreases when the culture ages. The gene copy number (GCN) of domains sxtA1 and sxtA4 was higher in the lag and stationary phase, and lag phase, respectively. No relationship was found between the GCN of the domains sxtA4 and sxtA1 with the PST content in G. catenatum.
    Keywords Gymnodinium ; gene dosage ; paralytic shellfish toxins
    Language English
    Dates of publication 2021-08
    Size p. 68-71.
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 204479-1
    ISSN 1879-3150 ; 0041-0101
    ISSN (online) 1879-3150
    ISSN 0041-0101
    DOI 10.1016/j.toxicon.2021.05.014
    Database NAL-Catalogue (AGRICOLA)

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