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Article ; Online: Genome-Wide Analysis of Antigen 43 (Ag43) Variants: New Insights in Their Diversity, Distribution and Prevalence in Bacteria.

Ageorges, Valentin / Wawrzyniak, Ivan / Ruiz, Philippe / Bicep, Cédric / Zorgani, Mohamed A / Paxman, Jason J / Heras, Begoña / Henderson, Ian R / Leroy, Sabine / Bailly, Xavier / Sapountzis, Panagiotis / Peyretaillade, Eric / Desvaux, Mickaël

International journal of molecular sciences

2023 Volume 24, Issue 6

Abstract: Antigen 43 (Ag43) expression induces aggregation and biofilm formation that has consequences for bacterial colonisation and infection. Ag43 is secreted through the Type 5 subtype "a" secretion system (T5aSS) and is a prototypical member of the family of ... ...

Abstract	Antigen 43 (Ag43) expression induces aggregation and biofilm formation that has consequences for bacterial colonisation and infection. Ag43 is secreted through the Type 5 subtype "a" secretion system (T5aSS) and is a prototypical member of the family of self-associating autotransporters (SAATs). As a T5aSS protein, Ag43 has a modular architecture comprised of (i) a signal peptide, (ii) a passenger domain that can be subdivided into three subdomains (SL, EJ, and BL), (iii) an autochaperone (AC) domain, and (iv) an outer membrane translocator. The cell-surface SL subdomain is directly involved in the "Velcro-handshake" mechanism resulting in bacterial autoaggregation. Ag43 is considered to have a ubiquitous distribution in
MeSH term(s)	Escherichia coli/physiology ; Escherichia coli Proteins/metabolism ; Adhesins, Escherichia coli/metabolism ; Bacterial Outer Membrane Proteins/genetics ; Phylogeny ; Prevalence
Chemical Substances	Escherichia coli Proteins ; Adhesins, Escherichia coli ; Bacterial Outer Membrane Proteins
Language	English
Publishing date	2023-03-13
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms24065500
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Article: Regulatory RNAs in the Less Studied Streptococcal Species: From Nomenclature to Identification.

Zorgani, Mohamed A / Quentin, Roland / Lartigue, Marie-Frédérique

Frontiers in microbiology

2016 Volume 7, Page(s) 1161

Abstract: Streptococcal species are Gram-positive bacteria involved in severe and invasive diseases in humans and animals. Although, this group includes different pathogenic species involved in life-threatening infections for humans, it also includes beneficial ... ...

Abstract	Streptococcal species are Gram-positive bacteria involved in severe and invasive diseases in humans and animals. Although, this group includes different pathogenic species involved in life-threatening infections for humans, it also includes beneficial species, such as Streptococcus thermophilus, which is used in yogurt production. In bacteria virulence factors are controlled by various regulatory networks including regulatory RNAs. For clearness and to develop logical thinking, we start this review with a revision of regulatory RNAs nomenclature. Previous reviews are mostly dealing with Streptococcus pyogenes and Streptococcus pneumoniae regulatory RNAs. We especially focused our analysis on regulatory RNAs in Streptococcus agalactiae, Streptococcus mutans, Streptococcus thermophilus and other less studied Streptococcus species. Although, S. agalactiae RNome remains largely unknown, sRNAs (small RNAs) are supposed to mediate regulation during environmental adaptation and host infection. In the case of S. mutans, sRNAs are suggested to be involved in competence regulation, carbohydrate metabolism, and Toxin-Antitoxin systems. A new category of miRNA-size small RNAs (msRNAs) was also identified for the first time in this species. The analysis of S. thermophilus sRNome shows that many sRNAs are associated to the bacterial immune system known as CRISPR-Cas system. Only few of the other different Streptococcus species have been the subject of studies pointed toward the characterization of regulatory RNAs. Finally, understanding bacterial sRNome can constitute one step forward to the elaboration of new strategies in therapy such as substitution of antibiotics in the management of S. agalactiae neonatal infections, prevention of S. mutans dental caries or use of S. thermophilus CRISPR-Cas system in genome editing applications.
Language	English
Publishing date	2016-07-26
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2587354-4
ISSN	1664-302X
ISSN	1664-302X
DOI	10.3389/fmicb.2016.01161
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Article ; Online: Whole Genome Sequence Analysis of the First Vancomycin-Resistant

Ahmed, Mohamed O / Elramalli, Asma K / Baptiste, Keith E / Daw, Mohamed A / Zorgani, Abdulaziz / Brouwer, Ellen / Willems, Rob J L / Top, Janetta

Microbial drug resistance (Larchmont, N.Y.)

2020 Volume 26, Issue 11, Page(s) 1390–1398

Abstract: The purpose of the study was to investigate the molecular characteristics and genetic relatedness of the first reported cases of vancomycin-resistant enterococci (VRE) from the Tripoli Medical Center, Libya. In total, 43 VRE isolates were obtained from ... ...

Abstract	The purpose of the study was to investigate the molecular characteristics and genetic relatedness of the first reported cases of vancomycin-resistant enterococci (VRE) from the Tripoli Medical Center, Libya. In total, 43 VRE isolates were obtained from various clinical sites throughout the years 2013-2014, including 40
MeSH term(s)	Anti-Bacterial Agents/pharmacology ; Bacterial Proteins/genetics ; Carbon-Oxygen Ligases/genetics ; Cross Infection/drug therapy ; Cross Infection/microbiology ; Disease Outbreaks ; Drug Resistance, Multiple, Bacterial/genetics ; Enterococcus/drug effects ; Enterococcus/genetics ; Enterococcus/isolation & purification ; Enterococcus faecium/genetics ; Enterococcus faecium/isolation & purification ; Gram-Positive Bacterial Infections/drug therapy ; Gram-Positive Bacterial Infections/microbiology ; Hospitals ; Humans ; Libya ; Multilocus Sequence Typing/methods ; Vancomycin/pharmacology ; Vancomycin-Resistant Enterococci/drug effects ; Vancomycin-Resistant Enterococci/genetics ; Vancomycin-Resistant Enterococci/isolation & purification ; Whole Genome Sequencing/methods
Chemical Substances	Anti-Bacterial Agents ; Bacterial Proteins ; Vancomycin (6Q205EH1VU) ; Carbon-Oxygen Ligases (EC 6.1.-)
Language	English
Publishing date	2020-03-16
Publishing country	United States
Document type	Journal Article
ZDB-ID	1290490-9
ISSN	1931-8448 ; 1076-6294
ISSN (online)	1931-8448
ISSN	1076-6294
DOI	10.1089/mdr.2019.0095
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Article ; Online: Genome-Wide Analysis of Antigen 43 (Ag43) Variants

Valentin Ageorges / Ivan Wawrzyniak / Philippe Ruiz / Cédric Bicep / Mohamed A. Zorgani / Jason J. Paxman / Begoña Heras / Ian R. Henderson / Sabine Leroy / Xavier Bailly / Panagiotis Sapountzis / Eric Peyretaillade / Mickaël Desvaux

International Journal of Molecular Sciences, Vol 24, Iss 5500, p

New Insights in Their Diversity, Distribution and Prevalence in Bacteria

2023 Volume 5500

Abstract: Antigen 43 (Ag43) expression induces aggregation and biofilm formation that has consequences for bacterial colonisation and infection. Ag43 is secreted through the Type 5 subtype “a” secretion system (T5aSS) and is a prototypical member of the family of ... ...

Abstract	Antigen 43 (Ag43) expression induces aggregation and biofilm formation that has consequences for bacterial colonisation and infection. Ag43 is secreted through the Type 5 subtype “a” secretion system (T5aSS) and is a prototypical member of the family of self-associating autotransporters (SAATs). As a T5aSS protein, Ag43 has a modular architecture comprised of (i) a signal peptide, (ii) a passenger domain that can be subdivided into three subdomains (SL, EJ, and BL), (iii) an autochaperone (AC) domain, and (iv) an outer membrane translocator. The cell-surface SL subdomain is directly involved in the “Velcro-handshake” mechanism resulting in bacterial autoaggregation. Ag43 is considered to have a ubiquitous distribution in E. coli genomes and many strains harbour multiple agn43 genes. However, recent phylogenetic analyses indicated the existence of four distinct Ag43 classes exhibiting different propensities for autoaggregation and interactions. Given the knowledge of the diversity and distribution of Ag43 in E. coli genomes is incomplete, we have performed a thorough in silico investigation across bacterial genomes. Our comprehensive analyses indicate that Ag43 passenger domains cluster in six phylogenetic classes associated with different SL subdomains. The diversity of Ag43 passenger domains is a result of the association of the SL subtypes with two different EJ-BL-AC modules. We reveal that agn43 is almost exclusively present among bacterial species of the Enterobacteriaceae family and essentially in the Escherichia genus (99.6%) but that it is not ubiquitous in E. coli . The gene is typically present as a single copy but up to five copies of agn43 with different combinations of classes can be observed. The presence of agn43 as well as its different classes appeared to differ between Escherichia phylogroups. Strikingly, agn43 is present in 90% of E. coli from E phylogroup. Our results shed light on Ag43 diversity and provide a rational framework for investigating its role in E. coli ecophysiology and ...
Keywords	Escherichia coli ; protein secretion ; autotransporter ; Type V protein secretion system (T5SS) ; phylogeny ; gene diversity ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
Subject code	572
Language	English
Publishing date	2023-03-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Editorial: Small Non-coding RNAs in

Zorgani, Mohamed A / Camiade, Emilie / Quentin, Roland / Lartigue, Marie-Frédérique

Frontiers in genetics

2016 Volume 7, Page(s) 192

Language	English
Publishing date	2016-11-02
Publishing country	Switzerland
Document type	Editorial
ZDB-ID	2606823-0
ISSN	1664-8021
ISSN	1664-8021
DOI	10.3389/fgene.2016.00192
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Article ; Online: Multidrug resistance and extended-spectrum β-lactamases genes among Escherichia coli from patients with urinary tract infections in Northwestern Libya.

Abujnah, Abubaker A / Zorgani, Abdulaziz / Sabri, Mohamed A M / El-Mohammady, Hanan / Khalek, Rania A / Ghenghesh, Khalifa S

The Libyan journal of medicine

2015 Volume 10, Issue 1, Page(s) 26412

Abstract: Introduction: Multidrug resistance (MDR) and emergence of extended-spectrum β-lactamases (ESBLs) that mediate resistance to β-lactam drugs among Escherichia coli and other uropathogens have been reported worldwide. However, there is little information ... ...

Abstract	Introduction: Multidrug resistance (MDR) and emergence of extended-spectrum β-lactamases (ESBLs) that mediate resistance to β-lactam drugs among Escherichia coli and other uropathogens have been reported worldwide. However, there is little information on the detection of ESBLs genes in E. coli from patients with urinary tract infections (UTIs) in the Arab countries using polymerase chain reaction (PCR), and in Libya such information is lacking. Methods: All patients attending Zawiya Teaching Hospital in Zawiya city between November 2012 and June 2013 suspected of having UTIs and from whom midstream urine samples were taken as part of the clinical workup were included in this prospective study. Samples were examined for uropathogens by standard bacteriological procedures. VITEK-2 automated microbiology system was used to identify the isolated uropathogens and determine the susceptibility of E. coli and Klebsiella spp. isolates to antimicrobials. In addition, phenotypically ESBLs-positive E. coli isolates were tested for ESBLs genes by PCR. Results: The present study enrolled 1,790 patients with UTIs. Uropathogens were found in 371 (20.7%) urine specimens examined. Mixed pathogens were detected in two specimens with 373 total pathogens isolated. E. coli and Klebsiella spp. were the predominant uropathogens at 55.8% (208/373) and 18.5% (69/373), respectively. Other pathogens were detected in 25.7% (96/373) of urine samples. Of the E. coli and Klebsiella spp. tested, 69.2 and 100% were resistant to ampicillin, 6.7 and 33.3% to ceftriaxone, and 23.1 and 17.4% to ciprofloxacin, respectively. MDR (resistance to ≥3 antimicrobial groups) was found in 69 (33.2%) of E. coli and in 29 (42%) of Klebsiella spp. isolates. ESBLs were detected phenotypically in 14 (6.7%) of E. coli and in 15 (21.7%) of Klebsiella spp. isolates. Thirteen out of the 14 phenotypically ESBL-positive E. coli were positive for ESBL genes by PCR. bla TEM gene was detected in seven isolates, bla OXA gene in 10 isolates and bla CTX-M gene in six isolates. bla SHV gene was not detected in the present study. Conclusion: The isolation of MDR ESBL-producing uropathogens undoubtedly will limit the choices clinicians have to treat their patients with UTIs. Therefore, there is an urgent need for surveillance studies on antimicrobial resistance and prevalence of ESBLs among uropathogens to guide the clinical treatment of UTIs in Libya in the future.
MeSH term(s)	Drug Resistance, Multiple, Bacterial ; Escherichia coli/enzymology ; Escherichia coli/isolation & purification ; Escherichia coli Infections/drug therapy ; Escherichia coli Infections/enzymology ; Escherichia coli Infections/epidemiology ; Female ; Humans ; Klebsiella Infections/drug therapy ; Klebsiella Infections/enzymology ; Klebsiella Infections/epidemiology ; Klebsiella pneumoniae/enzymology ; Klebsiella pneumoniae/isolation & purification ; Libya/epidemiology ; Male ; Microbial Sensitivity Tests ; Polymerase Chain Reaction ; Practice Guidelines as Topic ; Prevalence ; Prospective Studies ; Sentinel Surveillance ; Urinary Tract Infections/drug therapy ; Urinary Tract Infections/enzymology ; Urinary Tract Infections/epidemiology ; beta-Lactam Resistance ; beta-Lactamases/drug effects ; beta-Lactamases/genetics
Chemical Substances	beta-Lactamases (EC 3.5.2.6)
Language	English
Publishing date	2015-02-02
Publishing country	United States
Document type	Journal Article
ZDB-ID	2273005-9
ISSN	1819-6357 ; 1819-6357
ISSN (online)	1819-6357
ISSN	1819-6357
DOI	10.3402/ljm.v10.26412
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Article: Proteinaceous determinants of surface colonization in bacteria: bacterial adhesion and biofilm formation from a protein secretion perspective.

Chagnot, Caroline / Zorgani, Mohamed A / Astruc, Thierry / Desvaux, Mickaël

Frontiers in microbiology

2013 Volume 4, Page(s) 303

Abstract: Bacterial colonization of biotic or abiotic surfaces results from two quite distinct physiological processes, namely bacterial adhesion and biofilm formation. Broadly speaking, a biofilm is defined as the sessile development of microbial cells. Biofilm ... ...

Abstract	Bacterial colonization of biotic or abiotic surfaces results from two quite distinct physiological processes, namely bacterial adhesion and biofilm formation. Broadly speaking, a biofilm is defined as the sessile development of microbial cells. Biofilm formation arises following bacterial adhesion but not all single bacterial cells adhering reversibly or irreversibly engage inexorably into a sessile mode of growth. Among molecular determinants promoting bacterial colonization, surface proteins are the most functionally diverse active components. To be present on the bacterial cell surface, though, a protein must be secreted in the first place. Considering the close association of secreted proteins with their cognate secretion systems, the secretome (which refers both to the secretion systems and their protein substrates) is a key concept to apprehend the protein secretion and related physiological functions. The protein secretion systems are here considered in light of the differences in the cell-envelope architecture between diderm-LPS (archetypal Gram-negative), monoderm (archetypal Gram-positive) and diderm-mycolate (archetypal acid-fast) bacteria. Besides, their cognate secreted proteins engaged in the bacterial colonization process are regarded from single protein to supramolecular protein structure as well as the non-classical protein secretion. This state-of-the-art on the complement of the secretome (the secretion systems and their cognate effectors) involved in the surface colonization process in diderm-LPS and monoderm bacteria paves the way for future research directions in the field.
Language	English
Publishing date	2013-10-14
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2587354-4
ISSN	1664-302X
ISSN	1664-302X
DOI	10.3389/fmicb.2013.00303
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Article ; Online: Multidrug resistance and extended-spectrum β-lactamases genes among Escherichia coli from patients with urinary tract infections in Northwestern Libya

Abubaker A. Abujnah / Abdulaziz Zorgani / Mohamed A. M. Sabri / Hanan El-Mohammady / Rania A. Khalek / Khalifa S. Ghenghesh

Libyan Journal of Medicine, Vol 10, Iss 0, Pp 1-

2015 Volume 7

Abstract	Introduction: Multidrug resistance (MDR) and emergence of extended-spectrum β-lactamases (ESBLs) that mediate resistance to β-lactam drugs among Escherichia coli and other uropathogens have been reported worldwide. However, there is little information on the detection of ESBLs genes in E. coli from patients with urinary tract infections (UTIs) in the Arab countries using polymerase chain reaction (PCR), and in Libya such information is lacking. Methods: All patients attending Zawiya Teaching Hospital in Zawiya city between November 2012 and June 2013 suspected of having UTIs and from whom midstream urine samples were taken as part of the clinical workup were included in this prospective study. Samples were examined for uropathogens by standard bacteriological procedures. VITEK-2 automated microbiology system was used to identify the isolated uropathogens and determine the susceptibility of E. coli and Klebsiella spp. isolates to antimicrobials. In addition, phenotypically ESBLs-positive E. coli isolates were tested for ESBLs genes by PCR. Results: The present study enrolled 1,790 patients with UTIs. Uropathogens were found in 371 (20.7%) urine specimens examined. Mixed pathogens were detected in two specimens with 373 total pathogens isolated. E. coli and Klebsiella spp. were the predominant uropathogens at 55.8% (208/373) and 18.5% (69/373), respectively. Other pathogens were detected in 25.7% (96/373) of urine samples. Of the E. coli and Klebsiella spp. tested, 69.2 and 100% were resistant to ampicillin, 6.7 and 33.3% to ceftriaxone, and 23.1 and 17.4% to ciprofloxacin, respectively. MDR (resistance to ≥3 antimicrobial groups) was found in 69 (33.2%) of E. coli and in 29 (42%) of Klebsiella spp. isolates. ESBLs were detected phenotypically in 14 (6.7%) of E. coli and in 15 (21.7%) of Klebsiella spp. isolates. Thirteen out of the 14 phenotypically ESBL-positive E. coli were positive for ESBL genes by PCR. blaTEM gene was detected in seven isolates, blaOXA gene in 10 isolates and blaCTX-M gene in six isolates. ...
Keywords	urinary tract infections ; Escherichia coli ; extended-spectrum β-lactamases ; multidrug resistance ; Libya ; Medicine ; R
Subject code	610
Language	English
Publishing date	2015-02-01T00:00:00Z
Publisher	Taylor & Francis Group
Document type	Article ; Online
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Article ; Online: The Adc/Lmb System Mediates Zinc Acquisition in Streptococcus agalactiae and Contributes to Bacterial Growth and Survival.

Moulin, Pauline / Patron, Kévin / Cano, Camille / Zorgani, Mohamed Amine / Camiade, Emilie / Borezée-Durant, Elise / Rosenau, Agnès / Mereghetti, Laurent / Hiron, Aurélia

Journal of bacteriology

2016 Volume 198, Issue 24, Page(s) 3265–3277

Abstract: The Lmb protein of Streptococcus agalactiae is described as an adhesin that binds laminin, a component of the human extracellular matrix. In this study, we revealed a new role for this protein in zinc uptake. We also identified two Lmb homologs, AdcA and ...

Abstract	The Lmb protein of Streptococcus agalactiae is described as an adhesin that binds laminin, a component of the human extracellular matrix. In this study, we revealed a new role for this protein in zinc uptake. We also identified two Lmb homologs, AdcA and AdcAII, redundant binding proteins that combine with the AdcCB translocon to form a zinc-ABC transporter. Expression of this transporter is controlled by the zinc concentration in the medium through the zinc-dependent regulator AdcR. Triple deletion of lmb, adcA, and adcAII, or that of the adcCB genes, impaired growth and cell separation in a zinc-restricted environment. Moreover, we found that this Adc zinc-ABC transporter promotes S. agalactiae growth and survival in some human biological fluids, suggesting that it contributes to the infection process. These results indicated that zinc has biologically vital functions in S. agalactiae and that, under the conditions tested, the Adc/Lmb transporter constitutes the main zinc acquisition system of the bacterium. Importance: A zinc transporter, composed of three redundant binding proteins (Lmb, AdcA, and AdcAII), was characterized in Streptococcus agalactiae This system was shown to be essential for bacterial growth and morphology in zinc-restricted environments, including human biological fluids.
MeSH term(s)	ATP-Binding Cassette Transporters/metabolism ; Bacterial Proteins/metabolism ; Biological Transport ; Gene Expression Regulation, Bacterial ; Microbial Viability ; Streptococcus agalactiae/genetics ; Streptococcus agalactiae/growth & development ; Streptococcus agalactiae/metabolism ; Zinc/metabolism
Chemical Substances	AdcA protein, Streptococcus ; Bacterial Proteins ; Zinc (J41CSQ7QDS)
Language	English
Publishing date	2016-12-15
Publishing country	United States
Document type	Journal Article
ZDB-ID	2968-3
ISSN	1098-5530 ; 0021-9193
ISSN (online)	1098-5530
ISSN	0021-9193
DOI	10.1128/JB.00614-16
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Article: Identification of the Autochaperone Domain in the Type Va Secretion System (T5aSS): Prevalent Feature of Autotransporters with a β-Helical Passenger.

Rojas-Lopez, Maricarmen / Zorgani, Mohamed A / Kelley, Lawrence A / Bailly, Xavier / Kajava, Andrey V / Henderson, Ian R / Polticelli, Fabio / Pizza, Mariagrazia / Rosini, Roberto / Desvaux, Mickaël

Frontiers in microbiology

2018 Volume 8, Page(s) 2607

Abstract: Autotransporters (ATs) belong to a family of modular proteins secreted by the Type V, subtype a, secretion system (T5aSS) and considered as an important source of virulence factors in lipopolysaccharidic diderm bacteria (archetypical Gram-negative ... ...

Abstract	Autotransporters (ATs) belong to a family of modular proteins secreted by the Type V, subtype a, secretion system (T5aSS) and considered as an important source of virulence factors in lipopolysaccharidic diderm bacteria (archetypical Gram-negative bacteria). While exported by the Sec pathway, the ATs are further secreted across the outer membrane via their own C-terminal translocator forming a β-barrel, through which the rest of the protein, namely the passenger, can pass. In several ATs, an autochaperone domain (AC) present at the C-terminal region of the passenger and upstream of the translocator was demonstrated as strictly required for proper secretion and folding. However, considering it was functionally characterised and identified only in a handful of ATs, wariness recently fells on the commonality and conservation of this structural element in the T5aSS. To circumvent the issue of sequence divergence and taking advantage of the resolved three-dimensional structure of some ACs, identification of this domain was performed following structural alignment among all AT passengers experimentally resolved by crystallography before searching in a dataset of 1523 ATs. While demonstrating that the AC is indeed a conserved structure found in numerous ATs, phylogenetic analysis further revealed a distribution into deeply rooted branches, from which emerge 20 main clusters. Sequence analysis revealed that an AC could be identified in the large majority of SAATs (self-associating ATs) but not in any LEATs (lipase/esterase ATs) nor in some PATs (protease autotransporters) and PHATs (phosphatase/hydrolase ATs). Structural analysis indicated that an AC was present in passengers exhibiting single-stranded right-handed parallel β-helix, whatever the type of β-solenoid, but not with α-helical globular fold. From this investigation, the AC of type 1 appears as a prevalent and conserved structural element exclusively associated to β-helical AT passenger and should promote further studies about the protein secretion and folding via the T5aSS, especially toward α-helical AT passengers.
Language	English
Publishing date	2018-01-05
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587354-4
ISSN	1664-302X
ISSN	1664-302X
DOI	10.3389/fmicb.2017.02607
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