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  1. Article: Efficacy of homemade face masks against human coughs: Insights on penetration, atomization, and aerosolization of cough droplets.

    Krishan, Bal / Gupta, Dipendra / Vadlamudi, Gautham / Sharma, Shubham / Chakravortty, Dipshikha / Basu, Saptarshi

    Physics of fluids (Woodbury, N.Y. : 1994)

    2021  Volume 33, Issue 9, Page(s) 93309

    Abstract: Ever since the emergence of the ongoing COVID-19 pandemic, the usage of makeshift facemasks is generally advised by policymakers as a possible substitute for commercially available surgical or N95 face masks. Although such endorsements could be ... ...

    Abstract Ever since the emergence of the ongoing COVID-19 pandemic, the usage of makeshift facemasks is generally advised by policymakers as a possible substitute for commercially available surgical or N95 face masks. Although such endorsements could be economical and easily accessible in various low per-capita countries, the experimental evidence on the effectiveness of such recommendations is still lacking. In this regard, we carried out a detailed experimental investigation to study the fate of a large-sized surrogate cough droplet impingement at different velocities (corresponding to mild to severe coughs) on various locally procured cloth fabrics. Observation shows that larger ejected droplets (droplets that would normally settle as fomites in general) during a coughing event have enough momentum to penetrate single-layer cloth masks; the penetrated volume atomize into smaller daughter droplets that fall within aerosol range, thereby increasing infection potential. Theoretically, two essential criteria based on the balances of viscous dissipation-kinetic energy and surface tension-kinetic energy effects have been suggested for the droplet penetration through mask layers. Furthermore, a new parameter called
    Language English
    Publishing date 2021-09-14
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1472743-2
    ISSN 1089-7666 ; 1070-6631
    ISSN (online) 1089-7666
    ISSN 1070-6631
    DOI 10.1063/5.0061007
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Pediatric patient with fibrinogen Villeurbanne II presenting with an unprovoked portal vein thrombosis.

    Francisco, Brenton J / Sharma, Bal Krishan / Russell, Hannah M / Rosenfeldt, Leah / Owens, A Phillip / Flick, Matthew J / Mullins, Eric S / Palumbo, Joseph

    Blood advances

    2022  Volume 6, Issue 14, Page(s) 4297–4300

    MeSH term(s) Child ; Fibrinogen ; Humans ; Liver Diseases ; Portal Vein ; Venous Thrombosis/etiology
    Chemical Substances Fibrinogen (9001-32-5)
    Language English
    Publishing date 2022-07-25
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2915908-8
    ISSN 2473-9537 ; 2473-9529
    ISSN (online) 2473-9537
    ISSN 2473-9529
    DOI 10.1182/bloodadvances.2022006992
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Cancer-Associated Thrombosis: A Two-Way Street.

    Sharma, Bal Krishan / Flick, Matthew J / Palumbo, Joseph S

    Seminars in thrombosis and hemostasis

    2019  Volume 45, Issue 6, Page(s) 559–568

    Abstract: Pathological activation of the coagulation system occurs with virtually all forms of cancer, particularly epithelial malignancies. Accordingly, thrombosis is one of the most common comorbidities associated with cancer. Indeed, cancer-associated ... ...

    Abstract Pathological activation of the coagulation system occurs with virtually all forms of cancer, particularly epithelial malignancies. Accordingly, thrombosis is one of the most common comorbidities associated with cancer. Indeed, cancer-associated thromboembolism is the second leading cause of death for cancer patients, second only to the cancer itself. The identification of specific molecular mechanisms whereby tumor cells activate the coagulation system and drive thrombosis has been an active area of investigation for several decades. Studies in animal models and human trials have revealed that there is a bidirectional relationship between coagulation factor activity and cancer, whereby the pathological hemostatic system activation associated with cancer not only promotes thromboembolism but also drives progression of the malignancy. Numerous studies indicate that factors up and down the clotting cascade can contribute to various stages of cancer, including tumorigenesis, primary tumor growth, and metastasis. Although there are some mechanistic points of commonality, there are also clearly context-dependent contributions of coagulation components to cancer progression dependent on the type of cancer and stage of disease. It is also notable that in some instances, coagulation factors appear to contribute to cancer progression independently of their traditional roles in hemostasis and thrombosis. Here, the authors review the current state of the field with regard to hemostatic factor-driven cancer pathogenesis.
    MeSH term(s) Humans ; Neoplasms/complications ; Thrombosis/etiology
    Language English
    Publishing date 2019-08-05
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 196901-8
    ISSN 1098-9064 ; 0094-6176
    ISSN (online) 1098-9064
    ISSN 0094-6176
    DOI 10.1055/s-0039-1693472
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Evaluation of a Lipopolysaccharide and Resiquimod Combination as an Adjuvant with Inactivated Newcastle Disease Virus Vaccine in Chickens

    Bal Krishan Sharma / Saravanan Ramakrishnan / Abinaya Kaliappan / Mithilesh Singh / Ajay Kumar / Satyabrata Dandapat / Sohini Dey / Madhan Mohan Chellappa

    Vaccines, Vol 10, Iss 894, p

    2022  Volume 894

    Abstract: Various toll-like receptor (TLR) agonists have shown potential as adjuvants with different vaccines in both human and livestock species, including chickens. Our previous studies on combination of lipopolysaccharide (LPS; TLR4 agonist) and resiquimod (R- ... ...

    Abstract Various toll-like receptor (TLR) agonists have shown potential as adjuvants with different vaccines in both human and livestock species, including chickens. Our previous studies on combination of lipopolysaccharide (LPS; TLR4 agonist) and resiquimod (R-848; TLR7 agonist) showed the synergistic up-regulation of pro-inflammatory Th1 and Th2 cytokines in chicken peripheral blood mononuclear cells (PMBCs). Hence, the present study aimed to explore the combined adjuvant effect of LPS and R-848 with inactivated Newcastle disease virus (NDV) vaccine in chickens. Two weeks-old SPF chickens were immunized with inactivated NDV vaccine along with a combination of LPS and R-848 as an adjuvant with suitable control groups. A booster dose was given two weeks later. Antibody responses were assessed by enzyme linked immunosorbent assay (ELISA) and hemagglutination inhibition (HI) test, while cell-mediated immune responses were analyzed by a lymphocyte transformation test (LTT) and flow cytometry following vaccination. Two weeks post-booster, the birds were challenged with a velogenic strain of NDV, and protection against clinical signs, mortality and virus shedding was analyzed. The results indicated that inactivated NDV vaccine with R-848 induced significantly higher humoral and cellular immune responses with 100% protection against mortality and viral shedding following a virulent NDV challenge. However, the combination of LPS and R-848 along with inactivated NDV vaccine produced poor humoral and cellular immune responses and could not afford protection against challenge infection and virus shedding when compared to the vaccine-alone group, indicating the deleterious effects of the combination on antigen-specific immune responses. In conclusion, the combination of LPS and R-848 showed the inhibitory effects on antigen-specific humoral, cellular and protective immune responses when used as an adjuvant with inactivated NDV vaccines in chickens. This inhibitory effect might have occurred due to systemic cytokine storm. A ...
    Keywords TLR4 ; TLR7 ; resiquimod ; LPS ; adjuvant ; combination ; Medicine ; R
    Subject code 630
    Language English
    Publishing date 2022-06-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: Effect of TLR agonist on infections bronchitis virus replication and cytokine expression in embryonated chicken eggs.

    Sharma, Bal Krishan / Kakker, Naresh Kumar / Bhadouriya, Sakshi / Chhabra, Rajesh

    Molecular immunology

    2020  Volume 120, Page(s) 52–60

    Abstract: Avian infectious bronchitis (IB) is an acute, highly infectious and contagious viral disease of chickens caused by avian infectious bronchitis virus (IBV) belonging to the genus Coronavirus and family Coronaviridae. It can affect all age groups of birds. ...

    Abstract Avian infectious bronchitis (IB) is an acute, highly infectious and contagious viral disease of chickens caused by avian infectious bronchitis virus (IBV) belonging to the genus Coronavirus and family Coronaviridae. It can affect all age groups of birds. The toll-like receptors (TLRs) are a major class of innate immune pattern recognition receptors that have a key role in immune response and defense against various infections.The TLRs are essential for initiation of innate immune responses and in the development of adaptive immune responses. An in ovo model was employed to study the antiviral activity of TLR ligands (Pam3CSK4, LPS and CpG ODN) on replication of IBV. It was hypothesized that optimum dose and specific timing of TLR ligands may reduce viral load of IBV in specific pathogen free (SPF) embryonated chicken eggs (ECEs). Further, the mechanism involved in the TLR-mediated antiviral response in chorioallantoic membrane (CAM) of ECEs was investigated. The ECEs of 9-11 days old were treated with different doses (high, intermediate and low) of TLR-2 (Pam3CSK4), TLR-4 (LPS) and TLR-21 (CpG ODN) ligands. In addition, to know the timing of TLR ligand treatment, six time intervals were analyzed viz. 36, 24 and 12 h prior to infection, time of infection (co-administration of TLR ligands and avian IBV) and 12 and 24 h post-IBV infection. For studying the relative expression of immuno-stimulatory genes (IFN-α, IFN-β, IFN-γ, IL-1β, iNOS and OAS) in CAM, TLR ligands were administered through intra-allantoicroute and CAM were collected at 4, 8 and 16 h post treatment. The results demonstrated that intermediate dose of all the three TLR ligands significantly reduced virus titers and used in the present study. However, the LPS reduced virus titer pre- and post-IBV infection but Pam3CSK4 and CpG ODN reduced only pre-IBV infection. Further analysis showed that TLR ligands induced IFN-γ, IL-1β and IFN stimulated genes viz. iNOS and OAS genes in CAM. The present study pointed towards the novel opportunities for rational design of LPS as immuno-stimulatory agent in chickens with reference to IBV. It may be speculated that in ovo administration of these TLR ligands may enhance resistance against viral infection in neonatal chicken and may contribute towards the development of more effective and safer vaccines including in ovo vaccines.
    MeSH term(s) Adjuvants, Immunologic/pharmacology ; Animals ; Antiviral Agents/pharmacology ; Avian Proteins/agonists ; Avian Proteins/immunology ; Chick Embryo ; Coronavirus Infections/immunology ; Coronavirus Infections/veterinary ; Coronavirus Infections/virology ; Cytokines/metabolism ; Gene Expression/drug effects ; Gene Expression/immunology ; Immunity, Innate ; Infectious bronchitis virus/immunology ; Infectious bronchitis virus/pathogenicity ; Infectious bronchitis virus/physiology ; Ligands ; Lipopeptides/pharmacology ; Lipopolysaccharides/pharmacology ; Oligodeoxyribonucleotides/pharmacology ; Poultry Diseases/immunology ; Poultry Diseases/prevention & control ; Poultry Diseases/virology ; Toll-Like Receptors/agonists ; Toll-Like Receptors/immunology ; Viral Load/drug effects ; Viral Load/immunology ; Virus Replication/drug effects ; Virus Replication/immunology
    Chemical Substances Adjuvants, Immunologic ; Antiviral Agents ; Avian Proteins ; CPG-oligonucleotide ; Cytokines ; Ligands ; Lipopeptides ; Lipopolysaccharides ; Oligodeoxyribonucleotides ; Pam(3)CSK(4) peptide ; Toll-Like Receptors
    Keywords covid19
    Language English
    Publishing date 2020-02-14
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 424427-8
    ISSN 1872-9142 ; 0161-5890
    ISSN (online) 1872-9142
    ISSN 0161-5890
    DOI 10.1016/j.molimm.2020.02.001
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Evaluation of a Lipopolysaccharide and Resiquimod Combination as an Adjuvant with Inactivated Newcastle Disease Virus Vaccine in Chickens.

    Sharma, Bal Krishan / Ramakrishnan, Saravanan / Kaliappan, Abinaya / Singh, Mithilesh / Kumar, Ajay / Dandapat, Satyabrata / Dey, Sohini / Chellappa, Madhan Mohan

    Vaccines

    2022  Volume 10, Issue 6

    Abstract: Various toll-like receptor (TLR) agonists have shown potential as adjuvants with different vaccines in both human and livestock species, including chickens. Our previous studies on combination of lipopolysaccharide (LPS; TLR4 agonist) and resiquimod (R- ... ...

    Abstract Various toll-like receptor (TLR) agonists have shown potential as adjuvants with different vaccines in both human and livestock species, including chickens. Our previous studies on combination of lipopolysaccharide (LPS; TLR4 agonist) and resiquimod (R-848; TLR7 agonist) showed the synergistic up-regulation of pro-inflammatory Th1 and Th2 cytokines in chicken peripheral blood mononuclear cells (PMBCs). Hence, the present study aimed to explore the combined adjuvant effect of LPS and R-848 with inactivated Newcastle disease virus (NDV) vaccine in chickens. Two weeks-old SPF chickens were immunized with inactivated NDV vaccine along with a combination of LPS and R-848 as an adjuvant with suitable control groups. A booster dose was given two weeks later. Antibody responses were assessed by enzyme linked immunosorbent assay (ELISA) and hemagglutination inhibition (HI) test, while cell-mediated immune responses were analyzed by a lymphocyte transformation test (LTT) and flow cytometry following vaccination. Two weeks post-booster, the birds were challenged with a velogenic strain of NDV, and protection against clinical signs, mortality and virus shedding was analyzed. The results indicated that inactivated NDV vaccine with R-848 induced significantly higher humoral and cellular immune responses with 100% protection against mortality and viral shedding following a virulent NDV challenge. However, the combination of LPS and R-848 along with inactivated NDV vaccine produced poor humoral and cellular immune responses and could not afford protection against challenge infection and virus shedding when compared to the vaccine-alone group, indicating the deleterious effects of the combination on antigen-specific immune responses. In conclusion, the combination of LPS and R-848 showed the inhibitory effects on antigen-specific humoral, cellular and protective immune responses when used as an adjuvant with inactivated NDV vaccines in chickens. This inhibitory effect might have occurred due to systemic cytokine storm. A nanoparticle-based delivery of the combination of LPS and R-848 for slow and sustained release could be tried as an alternative method to explore the synergistic effect of the combination as an adjuvant in chickens.
    Language English
    Publishing date 2022-06-03
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2703319-3
    ISSN 2076-393X
    ISSN 2076-393X
    DOI 10.3390/vaccines10060894
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Isolation and Culturing Primary Chaolangiocytes from Mouse Liver.

    Kudira, Ramesh / Sharma, Bal Krishan / Mullen, Mary / Mohanty, Sujit K / Donnelly, Bryan / Tiao, Gregory M / Miethke, Alexander

    Bio-protocol

    2021  Volume 11, Issue 20, Page(s) e4192

    Abstract: Cholangiocytes are epithelial cells lining the intrahepatic and extrahepatic bile ducts. Cholangiocytes perform key physiological functions in the liver. Bile synthesized by hepatocytes is secreted into bile canaliculi, further stored in the gallbladder, ...

    Abstract Cholangiocytes are epithelial cells lining the intrahepatic and extrahepatic bile ducts. Cholangiocytes perform key physiological functions in the liver. Bile synthesized by hepatocytes is secreted into bile canaliculi, further stored in the gallbladder, and finally discharged into the duodenum. Due to liver injury, biliary epithelial proliferate in response to endogenous or exogenous signals leading to cholangiopathies, inflammation, fibrosis, and cholangiocarcinoma. Cholangiocytes exhibit anatomical and functional heterogeneity, and understanding such diversified functions will potentially help in finding effective therapies for various cholestatic liver diseases. To perform such functional studies, effective cholangiocyte isolation and culture procedures are needed. This protocol will aid in easy isolation and expansion of cholangiocytes from the liver.
    Language English
    Publishing date 2021-10-20
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2833269-6
    ISSN 2331-8325 ; 2331-8325
    ISSN (online) 2331-8325
    ISSN 2331-8325
    DOI 10.21769/BioProtoc.4192
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Factor XII promotes the thromboinflammatory response in a rat model of venoarterial extracorporeal membrane oxygenation.

    Kharnaf, Mousa / Zafar, Farhan / Hogue, Spencer / Rosenfeldt, Leah / Cantrell, Rachel L / Sharma, Bal Krishan / Pearson, Amelia / Sprague, Cassandra / Leino, Daniel / Abplanalp, William A / Zelek, Wioleta M / McCrae, Keith R / Shim, Young Jun / Morales, David / Tweddell, James / Qualls, Joseph E / Palumbo, Joseph S

    The Journal of thoracic and cardiovascular surgery

    2023  

    Abstract: Background: Factor XII (FXII) is a multifunctional protease capable of activating thrombotic and inflammatory pathways. FXII has been linked to thrombosis in extracorporeal membrane oxygenation (ECMO), but the role of FXII in ECMO-induced inflammatory ... ...

    Abstract Background: Factor XII (FXII) is a multifunctional protease capable of activating thrombotic and inflammatory pathways. FXII has been linked to thrombosis in extracorporeal membrane oxygenation (ECMO), but the role of FXII in ECMO-induced inflammatory complications has not been studied. We used novel gene-targeted FXII- deficient rats to evaluate the role of FXII in ECMO-induced thromboinflammation.
    Methods: FXII-deficient (FXII
    Results: FXII
    Conclusions: FXII initiates hemostatic system activation and key inflammatory sequelae in ECMO, suggesting that therapies targeting FXII could limit both thromboembolism and inopportune inflammatory complications in this setting.
    Language English
    Publishing date 2023-09-07
    Publishing country United States
    Document type Journal Article
    ZDB-ID 3104-5
    ISSN 1097-685X ; 0022-5223
    ISSN (online) 1097-685X
    ISSN 0022-5223
    DOI 10.1016/j.jtcvs.2023.08.045
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: Effect of TLR agonist on infections bronchitis virus replication and cytokine expression in embryonated chicken eggs

    Sharma, Bal Krishan / Kakker, Naresh Kumar / Bhadouriya, Sakshi / Chhabra, Rajesh

    Molecular immunology. 2020 Apr., v. 120

    2020  

    Abstract: Avian infectious bronchitis (IB) is an acute, highly infectious and contagious viral disease of chickens caused by avian infectious bronchitis virus (IBV) belonging to the genus Coronavirus and family Coronaviridae. It can affect all age groups of birds. ...

    Abstract Avian infectious bronchitis (IB) is an acute, highly infectious and contagious viral disease of chickens caused by avian infectious bronchitis virus (IBV) belonging to the genus Coronavirus and family Coronaviridae. It can affect all age groups of birds. The toll-like receptors (TLRs) are a major class of innate immune pattern recognition receptors that have a key role in immune response and defense against various infections.The TLRs are essential for initiation of innate immune responses and in the development of adaptive immune responses. An in ovo model was employed to study the antiviral activity of TLR ligands (Pam3CSK4, LPS and CpG ODN) on replication of IBV. It was hypothesized that optimum dose and specific timing of TLR ligands may reduce viral load of IBV in specific pathogen free (SPF) embryonated chicken eggs (ECEs). Further, the mechanism involved in the TLR-mediated antiviral response in chorioallantoic membrane (CAM) of ECEs was investigated. The ECEs of 9–11 days old were treated with different doses (high, intermediate and low) of TLR-2 (Pam3CSK4), TLR-4 (LPS) and TLR-21 (CpG ODN) ligands. In addition, to know the timing of TLR ligand treatment, six time intervals were analyzed viz. 36, 24 and 12 h prior to infection, time of infection (co-administration of TLR ligands and avian IBV) and 12 and 24 h post-IBV infection. For studying the relative expression of immuno-stimulatory genes (IFN-α, IFN-β, IFN-γ, IL-1β, iNOS and OAS) in CAM, TLR ligands were administered through intra-allantoicroute and CAM were collected at 4, 8 and 16 h post treatment. The results demonstrated that intermediate dose of all the three TLR ligands significantly reduced virus titers and used in the present study. However, the LPS reduced virus titer pre- and post-IBV infection but Pam3CSK4 and CpG ODN reduced only pre-IBV infection. Further analysis showed that TLR ligands induced IFN-γ, IL-1β and IFN stimulated genes viz. iNOS and OAS genes in CAM. The present study pointed towards the novel opportunities for rational design of LPS as immuno-stimulatory agent in chickens with reference to IBV. It may be speculated that in ovo administration of these TLR ligands may enhance resistance against viral infection in neonatal chicken and may contribute towards the development of more effective and safer vaccines including in ovo vaccines.
    Keywords Infectious bronchitis virus ; Toll-like receptor 4 ; adaptive immunity ; agonists ; antiviral properties ; bronchitis ; chicken eggs ; chickens ; chorioallantoic membrane ; genes ; immune response ; immunostimulants ; inducible nitric oxide synthase ; infectious bronchitis ; innate immunity ; interferon-alpha ; interferon-beta ; interferon-gamma ; interleukin-1beta ; ligands ; models ; oligodeoxyribonucleotides ; vaccines ; viral load ; virus replication ; covid19
    Language English
    Dates of publication 2020-04
    Size p. 52-60.
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 424427-8
    ISSN 1872-9142 ; 0161-5890
    ISSN (online) 1872-9142
    ISSN 0161-5890
    DOI 10.1016/j.molimm.2020.02.001
    Database NAL-Catalogue (AGRICOLA)

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  10. Article: Cancer-Associated Thrombosis: A Two-Way Street

    Sharma, Bal Krishan / Flick, Matthew J. / Palumbo, Joseph S.

    Seminars in Thrombosis and Hemostasis

    (Emergent Paradigms of Thrombosis and Cancer—Part II)

    2019  Volume 45, Issue 06, Page(s) 559–568

    Abstract: Pathological activation of the coagulation system occurs with virtually all forms of cancer, particularly epithelial malignancies. Accordingly, thrombosis is one of the most common comorbidities associated with cancer. Indeed, cancer-associated ... ...

    Series title Emergent Paradigms of Thrombosis and Cancer—Part II
    Abstract Pathological activation of the coagulation system occurs with virtually all forms of cancer, particularly epithelial malignancies. Accordingly, thrombosis is one of the most common comorbidities associated with cancer. Indeed, cancer-associated thromboembolism is the second leading cause of death for cancer patients, second only to the cancer itself. The identification of specific molecular mechanisms whereby tumor cells activate the coagulation system and drive thrombosis has been an active area of investigation for several decades. Studies in animal models and human trials have revealed that there is a bidirectional relationship between coagulation factor activity and cancer, whereby the pathological hemostatic system activation associated with cancer not only promotes thromboembolism but also drives progression of the malignancy. Numerous studies indicate that factors up and down the clotting cascade can contribute to various stages of cancer, including tumorigenesis, primary tumor growth, and metastasis. Although there are some mechanistic points of commonality, there are also clearly context-dependent contributions of coagulation components to cancer progression dependent on the type of cancer and stage of disease. It is also notable that in some instances, coagulation factors appear to contribute to cancer progression independently of their traditional roles in hemostasis and thrombosis. Here, the authors review the current state of the field with regard to hemostatic factor-driven cancer pathogenesis.
    Keywords cancer ; tissue factor ; thrombin ; fibrinogen
    Language English
    Publishing date 2019-08-05
    Publisher Thieme Medical Publishers
    Publishing place Stuttgart ; New York
    Document type Article
    ZDB-ID 196901-8
    ISSN 1098-9064 ; 0094-6176
    ISSN (online) 1098-9064
    ISSN 0094-6176
    DOI 10.1055/s-0039-1693472
    Database Thieme publisher's database

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