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Article ; Online: Powdered Cross-Linked Gelatin Methacryloyl as an Injectable Hydrogel for Adipose Tissue Engineering.

De Maeseneer, Tess / Van Damme, Lana / Aktan, Merve Kübra / Braem, Annabel / Moldenaers, Paula / Van Vlierberghe, Sandra / Cardinaels, Ruth

Gels (Basel, Switzerland)

2024 Volume 10, Issue 3

Abstract: The tissue engineering field is currently advancing towards minimally invasive procedures to reconstruct soft tissue defects. In this regard, injectable hydrogels are viewed as excellent scaffold candidates to support and promote the growth of ... ...

Abstract	The tissue engineering field is currently advancing towards minimally invasive procedures to reconstruct soft tissue defects. In this regard, injectable hydrogels are viewed as excellent scaffold candidates to support and promote the growth of encapsulated cells. Cross-linked gelatin methacryloyl (GelMA) gels have received substantial attention due to their extracellular matrix-mimicking properties. In particular, GelMA microgels were recently identified as interesting scaffold materials since the pores in between the microgel particles allow good cell movement and nutrient diffusion. The current work reports on a novel microgel preparation procedure in which a bulk GelMA hydrogel is ground into powder particles. These particles can be easily transformed into a microgel by swelling them in a suitable solvent. The rheological properties of the microgel are independent of the particle size and remain stable at body temperature, with only a minor reversible reduction in elastic modulus correlated to the unfolding of physical cross-links at elevated temperatures. Salts reduce the elastic modulus of the microgel network due to a deswelling of the particles, in addition to triple helix denaturation. The microgels are suited for clinical use, as proven by their excellent cytocompatibility. The latter is confirmed by the superior proliferation of encapsulated adipose tissue-derived stem cells in the microgel compared to the bulk hydrogel. Moreover, microgels made from the smallest particles are easily injected through a 20G needle, allowing a minimally invasive delivery. Hence, the current work reveals that powdered cross-linked GelMA is an excellent candidate to serve as an injectable hydrogel for adipose tissue engineering.
Language	English
Publishing date	2024-02-26
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2813982-3
ISSN	2310-2861 ; 2310-2861
ISSN (online)	2310-2861
ISSN	2310-2861
DOI	10.3390/gels10030167
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Equine Tenocyte Seeding on Gelatin Hydrogels Improves Elongated Morphology.

Meeremans, Marguerite / Van Damme, Lana / De Spiegelaere, Ward / Van Vlierberghe, Sandra / De Schauwer, Catharina

Polymers

2021 Volume 13, Issue 5

Abstract: 1) Background: Tendinopathy is a common injury in both human and equine athletes. Representative in vitro models are mandatory to facilitate translation of fundamental research into successful clinical treatments. Natural biomaterials like gelatin ... ...

Abstract	(1) Background: Tendinopathy is a common injury in both human and equine athletes. Representative in vitro models are mandatory to facilitate translation of fundamental research into successful clinical treatments. Natural biomaterials like gelatin provide favorable cell binding characteristics and are easily modifiable. In this study, methacrylated gelatin (gel-MA) and norbornene-functionalized gelatin (gel-NB), crosslinked with 1,4-dithiotreitol (DTT) or thiolated gelatin (gel-SH) were compared. (2) Methods: The physicochemical properties (
Language	English
Publishing date	2021-02-28
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2527146-5
ISSN	2073-4360 ; 2073-4360
ISSN (online)	2073-4360
ISSN	2073-4360
DOI	10.3390/polym13050747
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Article ; Online: Toward Adipose Tissue Engineering Using Thiol-Norbornene Photo-Crosslinkable Gelatin Hydrogels.

Van Damme, Lana / Van Hoorick, Jasper / Blondeel, Philip / Van Vlierberghe, Sandra

Biomacromolecules

2021 Volume 22, Issue 6, Page(s) 2408–2418

Abstract: Nowadays, breast implants, lipofilling, and microsurgical free tissue transfer are the most often applied procedures to repair soft tissue defects resulting from mastectomies/lumpectomies following breast cancer. Due to the drawbacks and limitations ... ...

Abstract	Nowadays, breast implants, lipofilling, and microsurgical free tissue transfer are the most often applied procedures to repair soft tissue defects resulting from mastectomies/lumpectomies following breast cancer. Due to the drawbacks and limitations associated with these conventional clinical practices, there is a need for alternative reconstructive strategies. The development of biomimetic materials able to promote cell proliferation and adipogenic differentiation has gained increasing attention in the context of adipose reconstructive purposes. Herein, thiol-norbornene crosslinkable gelatin-based materials were developed and benchmarked to the current commonly applied methacryloyl-modified gelatin (GelMA) with different degrees of substitutions focussing on bottom-up tissue engineering. The developed hydrogels resulted in similar gel fractions, swelling, and in vitro biodegradation properties compared to the benchmark materials. Furthermore, the thiol-ene hydrogels exhibited mechanical properties closer to those of native fatty tissue compared to GelMA. The mechanical cues of the equimolar GelNB DS55% + GelSH DS75% composition resulted not only in similar biocompatibility but also, more importantly, in superior differentiation of the encapsulated cells into the adipogenic lineage, as compared to GelMA. It can be concluded that the photo-crosslinkable thiol-ene systems offer a promising strategy toward adipose tissue engineering through cell encapsulation compared to the benchmark GelMA.
MeSH term(s)	Adipose Tissue ; Gelatin ; Hydrogels ; Norbornanes ; Sulfhydryl Compounds ; Tissue Engineering
Chemical Substances	Hydrogels ; Norbornanes ; Sulfhydryl Compounds ; Gelatin (9000-70-8)
Language	English
Publishing date	2021-05-05
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1526-4602
ISSN (online)	1526-4602
DOI	10.1021/acs.biomac.1c00189
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Toward Adipose Tissue Engineering Using Thiol-Norbornene Photo-Crosslinkable Gelatin Hydrogels

Van Damme, Lana / Van Hoorick, Jasper / Blondeel, Philip / Van Vlierberghe, Sandra

Biomacromolecules. 2021 May 05, v. 22, no. 6

2021

Abstract	Nowadays, breast implants, lipofilling, and microsurgical free tissue transfer are the most often applied procedures to repair soft tissue defects resulting from mastectomies/lumpectomies following breast cancer. Due to the drawbacks and limitations associated with these conventional clinical practices, there is a need for alternative reconstructive strategies. The development of biomimetic materials able to promote cell proliferation and adipogenic differentiation has gained increasing attention in the context of adipose reconstructive purposes. Herein, thiol-norbornene crosslinkable gelatin-based materials were developed and benchmarked to the current commonly applied methacryloyl-modified gelatin (GelMA) with different degrees of substitutions focussing on bottom-up tissue engineering. The developed hydrogels resulted in similar gel fractions, swelling, and in vitro biodegradation properties compared to the benchmark materials. Furthermore, the thiol-ene hydrogels exhibited mechanical properties closer to those of native fatty tissue compared to GelMA. The mechanical cues of the equimolar GelNB DS55% + GelSH DS75% composition resulted not only in similar biocompatibility but also, more importantly, in superior differentiation of the encapsulated cells into the adipogenic lineage, as compared to GelMA. It can be concluded that the photo-crosslinkable thiol-ene systems offer a promising strategy toward adipose tissue engineering through cell encapsulation compared to the benchmark GelMA.
Keywords	adipogenesis ; adipose tissue ; biocompatibility ; biodegradation ; biomimetics ; breast neoplasms ; breasts ; cell proliferation ; encapsulation ; gelatin ; hydrogels
Language	English
Dates of publication	2021-0505
Size	p. 2408-2418.
Publishing place	American Chemical Society
Document type	Article
Note	NAL-AP-2-clean
ISSN	1526-4602
DOI	10.1021/acs.biomac.1c00189
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Effect of decompressive laparotomy on organ function in patients with abdominal compartment syndrome: a systematic review and meta-analysis.

Van Damme, Lana / De Waele, Jan J

Critical care (London, England)

2018 Volume 22, Issue 1, Page(s) 179

Abstract: Background: Decompressive laparotomy has been advised as potential treatment for abdominal compartment syndrome (ACS) when medical management fails; yet, the effect on parameters of organ function differs markedly in the published literature. In this ... ...

Abstract	Background: Decompressive laparotomy has been advised as potential treatment for abdominal compartment syndrome (ACS) when medical management fails; yet, the effect on parameters of organ function differs markedly in the published literature. In this study, we sought to investigate the effect of decompressive laparotomy on intra-abdominal pressure and organ function in critically ill adult and pediatric patients with ACS, specifically focusing on hemodynamic, respiratory, and kidney function and outcome. Methods: A systematic review and meta-analysis of the literature was performed. Articles reporting data on intra-abdominal pressure (IAP), hemodynamic (mean arterial pressures [MAP], central venous pressure [CVP], cardiac index [CI], heart rate [HR], systemic vascular resistance index [SVRI] and/or pulmonary capillary wedge pressure [PCWP]), respiratory (positive end-expiratory pressure [PEEP], peak inspiratory pressure [PIP] and/or ratio of partial pressure arterial oxygen and fraction of inspired oxygen [P/F ratio]), and/or urinary output (UO) following decompressive laparotomy were analyzed. Results: A total of 15 articles were included; 3 included children only (aged 18 years or younger). Of the 286 patients who were included, 49.7% had primary ACS. The baseline mean IAP in adults decreased with an average of 18.2 ± 6.5 mmHg following decompression, from 31.7 ± 6.4 mmHg to 13.5 ± 3.0 mmHg. There was a decrease in HR (12.2 ± 9.5 beats/min; p = 0.04), CVP (4.6 ± 2.3 mmHg; p = 0.022), PCWP (5.8 ± 2.3 mmHg; p = 0.029), and PIP (10.1 ± 3.9 cmH Conclusions: Decompressive laparotomy resulted in a significantly lower IAP and had beneficial effects on hemodynamic, respiratory, and renal parameters. Mortality after decompressive laparotomy remains high in both adults and children.
MeSH term(s)	Critical Illness/therapy ; Decompression, Surgical/methods ; Decompression, Surgical/standards ; Humans ; Intra-Abdominal Hypertension/classification ; Intra-Abdominal Hypertension/surgery ; Laparotomy/methods ; Laparotomy/standards ; Lower Body Negative Pressure/methods ; Lower Body Negative Pressure/standards ; Organ Dysfunction Scores
Language	English
Publishing date	2018-07-25
Publishing country	England
Document type	Journal Article ; Meta-Analysis ; Systematic Review
ZDB-ID	2041406-7
ISSN	1466-609X ; 1364-8535
ISSN (online)	1466-609X
ISSN	1364-8535
DOI	10.1186/s13054-018-2103-0
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Equine Tenocyte Seeding on Gelatin Hydrogels Improves Elongated Morphology

Marguerite Meeremans / Lana Van Damme / Ward De Spiegelaere / Sandra Van Vlierberghe / Catharina De Schauwer

Polymers, Vol 13, Iss 5, p

2021 Volume 747

Abstract	(1) Background: Tendinopathy is a common injury in both human and equine athletes. Representative in vitro models are mandatory to facilitate translation of fundamental research into successful clinical treatments. Natural biomaterials like gelatin provide favorable cell binding characteristics and are easily modifiable. In this study, methacrylated gelatin (gel-MA) and norbornene-functionalized gelatin (gel-NB), crosslinked with 1,4-dithiotreitol (DTT) or thiolated gelatin (gel-SH) were compared. (2) Methods: The physicochemical properties ( 1 H-NMR spectroscopy, gel fraction, swelling ratio, and storage modulus) and equine tenocyte characteristics (proliferation, viability, and morphology) of four different hydrogels (gel-MA, gel-NB85/DTT, gel-NB55/DTT, and gel-NB85/SH75) were evaluated. Cellular functionality was analyzed using fluorescence microscopy (viability assay and focal adhesion staining). (3) Results: The thiol-ene based hydrogels showed a significantly lower gel fraction/storage modulus and a higher swelling ratio compared to gel-MA. Significantly less tenocytes were observed on gel-MA discs at 14 days compared to gel-NB85/DTT, gel-NB55/DTT and gel-NB85/SH75. At 7 and 14 days, the characteristic elongated morphology of tenocytes was significantly more pronounced on gel-NB85/DTT and gel-NB55/DTT in contrast to TCP and gel-MA. (4) Conclusions: Thiol-ene crosslinked gelatins exploiting DTT as a crosslinker are the preferred biomaterials to support the culture of tenocytes. Follow-up experiments will evaluate these biomaterials in more complex models.
Keywords	tenocytes ; hydrogels ; gelatin ; cell proliferation ; viability ; morphology ; Organic chemistry ; QD241-441
Subject code	620
Language	English
Publishing date	2021-02-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Photo-Crosslinked Gelatin-Based Hydrogel Films to Support Wound Healing.

Stubbe, Birgit / Mignon, Arn / Van Damme, Lana / Claes, Karel / Hoeksema, Henk / Monstrey, Stan / Van Vlierberghe, Sandra / Dubruel, Peter

Macromolecular bioscience

2021 Volume 21, Issue 12, Page(s) e2100246

Abstract: Gelatin is used widely in the biomedical field, among other for wound healing. Given its upper critical solution temperature, crosslinking is required. To this end, gelatin is chemically modified with different photo-crosslinkable moieties with low (32- ... ...

Abstract	Gelatin is used widely in the biomedical field, among other for wound healing. Given its upper critical solution temperature, crosslinking is required. To this end, gelatin is chemically modified with different photo-crosslinkable moieties with low (32-34%) and high (63-65%) degree of substitution (DS): gelatin-methacrylamide (gel-MA) and gelatin-acrylamide (gel-AA) and gelatin-pentenamide (gel-PE). Next to the more researched gel-MA, it is especially interesting and novel to compare with other gelatin-derived compounds for the application of wound healing. An additional comparison is made with commercial dressings. The DS is directly proportional to the mechanical characteristics and inversely proportional to the swelling capacity. Gel-PE shows weaker mechanical properties (G' < 15 kPa) than gel-AA and gel-MA (G' < 39 and 45 kPa, respectively). All derivatives are predominantly elastic (recovery indices of 89-94%). Gel-AA and gel-MA show excellent biocompatibility, whereas gel-PE shows a significantly lower initial biocompatibility, evolving positively toward day 7. Overall, gel-MA shows to have the most potential to be applied as wound dressing. Future blending with gel-AA to improve the curing kinetics can lead to dressings able to compete with current commercial dressings.
MeSH term(s)	Biocompatible Materials/chemistry ; Fibroblasts/metabolism ; Gelatin/chemistry ; Humans ; Materials Testing ; Methylgalactosides/chemistry ; Wound Healing
Chemical Substances	Biocompatible Materials ; Methylgalactosides ; hydrogel film ; Gelatin (9000-70-8)
Language	English
Publishing date	2021-09-29
Publishing country	Germany
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2039130-4
ISSN	1616-5195 ; 1616-5187
ISSN (online)	1616-5195
ISSN	1616-5187
DOI	10.1002/mabi.202100246
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Tough Photo-Cross-Linked PCL-Hydroxyapatite Composites for Bone Tissue Engineering.

Thijssen, Quinten / Cornelis, Kim / Alkaissy, Rand / Locs, Janis / Damme, Lana Van / Schaubroeck, David / Willaert, Robin / Snelling, Sarah / Mouthuy, Pierre-Alexis / Van Vlierberghe, Sandra

Biomacromolecules

2022 Volume 23, Issue 3, Page(s) 1366–1375

Abstract: Acrylate-based photo-cross-linked poly(ε-caprolactone) (PCL) tends to show low elongation and strength. Incorporation of osteo-inductive hydroxyapatite (HAp) further enhances this effect, which limits its applicability in bone tissue engineering. To ... ...

Abstract	Acrylate-based photo-cross-linked poly(ε-caprolactone) (PCL) tends to show low elongation and strength. Incorporation of osteo-inductive hydroxyapatite (HAp) further enhances this effect, which limits its applicability in bone tissue engineering. To overcome this, the thiol-ene click reaction is introduced for the first time in order to photo-cross-link PCL composites with 0, 10, 20, and 30 wt % HAp nanoparticles. It is demonstrated that the elongation at break and ultimate strength increase 10- and 2-fold, respectively, when the photopolymerization mechanism is shifted from a radical chain-growth (i.e., acrylate cross-linking) toward a radical step-growth polymerization (i.e., thiol-ene cross-linking). Additionally, it is illustrated that osteoblasts can attach to and proliferate on the surface of the photo-cross-linked PCL-HAp composites. Finally, the incorporation of HAp nanoparticles is shown to reduce the ALP activity of osteoblasts. Overall, thiol-ene cross-linked PCL-HAp composites can be considered as promising potential materials for bone tissue engineering.
MeSH term(s)	Durapatite ; Polyesters ; Sulfhydryl Compounds ; Tissue Engineering ; Tissue Scaffolds
Chemical Substances	Polyesters ; Sulfhydryl Compounds ; Durapatite (91D9GV0Z28)
Language	English
Publishing date	2022-02-11
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1526-4602
ISSN (online)	1526-4602
DOI	10.1021/acs.biomac.1c01584
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Vascularized adipose tissue engineering: moving towards soft tissue reconstruction.

Peirsman, Arne / Nguyen, Huu Tuan / Van Waeyenberge, Michiel / Ceballos, Carlos / Bolivar, Johana / Kawakita, Satoru / Vanlauwe, Florian / Tirpáková, Zuzana / Van Dorpe, Sofie / Van Damme, Lana / Mecwan, Marvin / Ermis, Menekse / Maity, Surjendu / Mandal, Kalpana / Herculano, Rondinelli / Depypere, Bernard / Budiharto, Lore / Van Vlierberghe, Sandra / De Wever, Olivier /

Blondeel, Phillip / Jucaud, Vadim / Dokmeci, Mehmet Remzi / Khademhosseini, Ali

Biofabrication

2023 Volume 15, Issue 3

Abstract: Soft tissue defects are a common clinical challenge mostly caused by trauma, congenital anomalies and oncological surgery. Current soft tissue reconstruction options include synthetic materials (fillers and implants) and autologous adipose tissue ... ...

Abstract	Soft tissue defects are a common clinical challenge mostly caused by trauma, congenital anomalies and oncological surgery. Current soft tissue reconstruction options include synthetic materials (fillers and implants) and autologous adipose tissue transplantation through flap surgery and/or lipotransfer. Both reconstructive options hold important disadvantages to which vascularized adipose tissue engineering (VATE) strategies could offer solutions. In this review, we first summarized pivotal characteristics of functional adipose tissue such as the structure, function, cell types, development and extracellular matrix (ECM). Next, we discussed relevant cell sources and how they are applied in different state-of-the-art VATE techniques. Herein, biomaterial scaffolds and hydrogels, ECMs, spheroids, organoids, cell sheets, three dimensional printing and microfluidics are overviewed. Also, we included extracellular vesicles and emphasized their potential role in VATE. Lastly, current challenges and future perspectives in VATE are pointed out to help to pave the road towards clinical applications.
MeSH term(s)	Tissue Scaffolds/chemistry ; Tissue Engineering/methods ; Adipose Tissue ; Biocompatible Materials ; Hydrogels
Chemical Substances	Biocompatible Materials ; Hydrogels
Language	English
Publishing date	2023-06-02
Publishing country	England
Document type	Journal Article ; Review
ZDB-ID	2500944-8
ISSN	1758-5090 ; 1758-5082
ISSN (online)	1758-5090
ISSN	1758-5082
DOI	10.1088/1758-5090/acd7a5
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Tough Photo-Cross-Linked PCL-Hydroxyapatite Composites for Bone Tissue Engineering

Thijssen, Quinten / Cornelis, Kim / Alkaissy, Rand / Locs, Janis / Damme, Lana Van / Schaubroeck, David / Willaert, Robin / Snelling, Sarah / Mouthuy, Pierre-Alexis / Van Vlierberghe, Sandra

Biomacromolecules. 2022 Feb. 11, v. 23, no. 3

2022

Abstract	Acrylate-based photo-cross-linked poly(ε-caprolactone) (PCL) tends to show low elongation and strength. Incorporation of osteo-inductive hydroxyapatite (HAp) further enhances this effect, which limits its applicability in bone tissue engineering. To overcome this, the thiol–ene click reaction is introduced for the first time in order to photo-cross-link PCL composites with 0, 10, 20, and 30 wt % HAp nanoparticles. It is demonstrated that the elongation at break and ultimate strength increase 10- and 2-fold, respectively, when the photopolymerization mechanism is shifted from a radical chain-growth (i.e., acrylate cross-linking) toward a radical step-growth polymerization (i.e., thiol–ene cross-linking). Additionally, it is illustrated that osteoblasts can attach to and proliferate on the surface of the photo-cross-linked PCL-HAp composites. Finally, the incorporation of HAp nanoparticles is shown to reduce the ALP activity of osteoblasts. Overall, thiol–ene cross-linked PCL-HAp composites can be considered as promising potential materials for bone tissue engineering.
Keywords	bones ; crosslinking ; hydroxyapatite ; osteoblasts ; polymerization
Language	English
Dates of publication	2022-0211
Size	p. 1366-1375.
Publishing place	American Chemical Society
Document type	Article
ISSN	1526-4602
DOI	10.1021/acs.biomac.1c01584
Database	NAL-Catalogue (AGRICOLA)

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