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  1. Article: Genetic improvement for blast resistance in high-yielding cold-tolerant rice (

    Rathour, Rajeev / Kumar, Rohit / Thakur, Kalpna / Pote, Tushar Diliprao

    3 Biotech

    2022  Volume 12, Issue 8, Page(s) 165

    Abstract: Blast disease and cold stress are two major yield-limiting factors for rice under temperate climates. Marker-assisted backcross breeding approach (MABB) was employed for the improvement of blast resistance in a popular cold-tolerant variety 'Himalaya741' ...

    Abstract Blast disease and cold stress are two major yield-limiting factors for rice under temperate climates. Marker-assisted backcross breeding approach (MABB) was employed for the improvement of blast resistance in a popular cold-tolerant variety 'Himalaya741' by introgressing a broad-spectrum resistance locus
    Supplementary information: The online version contains supplementary material available at 10.1007/s13205-022-03244-w.
    Language English
    Publishing date 2022-07-10
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2600522-0
    ISSN 2190-5738 ; 2190-572X
    ISSN (online) 2190-5738
    ISSN 2190-572X
    DOI 10.1007/s13205-022-03244-w
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Effect of the Pandemic and Lockdown on Mental Health of Children.

    Thakur, Kalpna / Kumar, Naveen / Sharma, NovRattan

    Indian journal of pediatrics

    2020  Volume 87, Issue 7, Page(s) 552

    MeSH term(s) COVID-19 ; Child ; Humans ; Mental Health ; Pandemics
    Keywords covid19
    Language English
    Publishing date 2020-05-12
    Publishing country India
    Document type Letter
    ZDB-ID 218231-2
    ISSN 0973-7693 ; 0019-5456
    ISSN (online) 0973-7693
    ISSN 0019-5456
    DOI 10.1007/s12098-020-03308-w
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 826: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  3. Article: Cholesterol Granuloma in Dentigerous Cyst: A Diagnostic Dilemma

    Bhat, Nitish / Thakur, Kalpna / Bhardwaj, Nandini / Nandan, Hemwati

    Dental Journal of Advance Studies

    2022  Volume 10, Issue 01, Page(s) 21–24

    Abstract: Cholesterol deposition is a common phenomenon to be seen associated to an inflammatory response and is usually evident in paranasal sinus, middle ear, and in some cases of mastoid process. Such depositions lead to cleft formations within the tissue, ... ...

    Abstract Cholesterol deposition is a common phenomenon to be seen associated to an inflammatory response and is usually evident in paranasal sinus, middle ear, and in some cases of mastoid process. Such depositions lead to cleft formations within the tissue, histopathologically seen as cholesterol cleft. Formation of cholesterol clefts is an usual phenomenon in the jaw related to inflammatory cysts such as radicular cyst but this is rarely seen in other odontogenic cysts, in particular the dentigerous cyst, odontogenic keratocyst. So, here we present a rare case of cholesterol clefts in the wall of a dentigerous cyst.
    Keywords cholesterol clefts ; dentigerous cyst ; odontogenic cyst ; cholesterol crystals
    Language English
    Publishing date 2022-04-01
    Publisher Thieme Medical and Scientific Publishers Pvt. Ltd.
    Publishing place Stuttgart ; New York
    Document type Article
    ISSN 2349-9869 ; 2321-1482
    ISSN (online) 2349-9869
    ISSN 2321-1482
    DOI 10.1055/s-0042-1750392
    Database Thieme publisher's database

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  5. Article: Development of tryptophan rich maize lines through simple sequence repeat marker aided introgression of Opaque2 trait

    Lata, Swaran / Kumari, Reena / Thakur, Kalpna / Rana, Maneet / Thakur, Naresh / Hussain, Waseem

    Electronic journal of plant breeding. 2022 June, v. 13, no. 2

    2022  

    Abstract: Maize is the main staple food of the world but it is nutritional deficient due to the scarcity of two essential amino acids viz., lysine and tryptophan. Under this study, opaque2 gene from QPM donor (CML-169) was transferred into normal maize (BAJIM-08- ... ...

    Abstract Maize is the main staple food of the world but it is nutritional deficient due to the scarcity of two essential amino acids viz., lysine and tryptophan. Under this study, opaque2 gene from QPM donor (CML-169) was transferred into normal maize (BAJIM-08-26) through marker aided backcross breeding method. The opaque2 specific SSR marker (phi057) was used for foreground selection in BC₁F₁ and BC₂F₁ generations. The heterozygous offspring were self-pollinated to produce the BC₂F₂ generation. Further plants on the basis of 25% opaqueness were selected and selfed to develop BC₂F₃ and BC₂F₄ progenies. The background selection using a series of SSR markers showed 97% recovery in the recurrent parent genome of backcrossed generation. Tryptophan content and total protein in the endosperm of BC₂F₄ progenies were found to be ranged from 0.75 to 0.93% and 7.0 to 9.6%, respectively. As an outcome, three promising MAS derived QPM lines viz., B76-22-2, B70-5-8 and B42-3-1 were developed that showed a high percentage of tryptophan, grain yield and recovery of recurrent parent genome. The worldwide problem of protein malnutrition could be improved by adopting a molecular breeding approach for the development of nutritionally rich maize over the normal maize.
    Keywords backcrossing ; corn ; endosperm ; genes ; grain yield ; heterozygosity ; introgression ; kwashiorkor ; lysine ; microsatellite repeats ; progeny ; protein content ; self-pollination ; staple foods ; tryptophan
    Language English
    Dates of publication 2022-06
    Size p. 295-304.
    Publishing place Indian Society of Plant Breeders
    Document type Article
    ZDB-ID 2534084-0
    ISSN 0975-928X
    ISSN 0975-928X
    DOI 10.37992/2022.1302.082
    Database NAL-Catalogue (AGRICOLA)

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  6. Article: Genetic improvement for blast resistance in high-yielding cold-tolerant rice (Oryza sativa L.) cultivar Himalaya 741 by marker-assisted backcross breeding

    Rathour, Rajeev / Kumar, Rohit / Thakur, Kalpna / Pote, Tushar Diliprao

    3 Biotech. 2022 Aug., v. 12, no. 8

    2022  

    Abstract: Blast disease and cold stress are two major yield-limiting factors for rice under temperate climates. Marker-assisted backcross breeding approach (MABB) was employed for the improvement of blast resistance in a popular cold-tolerant variety ‘Himalaya741’ ...

    Abstract Blast disease and cold stress are two major yield-limiting factors for rice under temperate climates. Marker-assisted backcross breeding approach (MABB) was employed for the improvement of blast resistance in a popular cold-tolerant variety ‘Himalaya741’ by introgressing a broad-spectrum resistance locus Pi9 from a Basmati donor PB1637. A combined use of phenotypic selection and marker-based genotypic selection ensured speedy reconstitution of the recurrent parent genome (RPG) in backcross progenies; RPG recovery in most of the progenies was > 96% with three progenies namely, HPU-1-33, -38 and -49 showing complete recovery of recurrent parent genome. Notwithstanding a very higher recovery rate of RPG in introgression lines, the lines still inherited a large linkage block > 13.3 Mb with Pi9 from the donor line PB1637. The donor chromosome segments co-inherited with Pi9 gene, however, did not have any adverse effect on the agronomic performance of the Pi9 introgression lines. Of the eight genetically superior Pi9 introgression lines identified, two exhibited resemblance to Himalaya 741 for most of the agronomic traits in addition to having superior grain length and tiller number. The introgression line HPU-1–81 displayed 44% yield superiority over recurrent parent, primarily due to improvement in yield-contributing traits, namely, tiller number, panicle length, thousand-seed-weight and grain length. All the Pi9 introgression lines displayed a high level of resistance comparable to PB1637 against two highly virulent blast races, which collectively displayed compatibility to 15 different major resistance genes. The introgression lines also possessed reproductive stage cold tolerance similar to recurrent parent under prevailing cold stress conditions. The agronomically superior Pi9 introgression lines developed herein are expected to provide a comparable or better substitute to blast susceptible variety Himalaya 741 for extenuating losses due to cold stress and blast disease.
    Keywords Oryza sativa ; adverse effects ; agronomic traits ; backcrossing ; blast disease ; chromosomes ; cold stress ; cold tolerance ; cultivars ; genetic improvement ; introgression ; panicles ; phenotypic selection ; resistance genes ; rice ; virulence ; Himalayan region
    Language English
    Dates of publication 2022-08
    Size p. 165.
    Publishing place Springer International Publishing
    Document type Article
    ZDB-ID 2600522-0
    ISSN 2190-5738 ; 2190-572X
    ISSN (online) 2190-5738
    ISSN 2190-572X
    DOI 10.1007/s13205-022-03244-w
    Database NAL-Catalogue (AGRICOLA)

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  7. Article: In Vitro Plant Regeneration by Organogenesis from Leaf Callus of Carnation, Dianthus caryophyllus L. cv. ‘Master’

    Thakur, Kalpna / Kamlesh Kanwar

    Proceedings of the National Academy of Sciences, India, Section B: biological sciences. 2018 Sept., v. 88, no. 3

    2018  

    Abstract: An efficient indirect in vitro plant regeneration protocol for Dianthus caryophyllus L. cv. ‘Master’ was developed using leaf explants. This study revealed the morphogenetic potential of leaf explant as a source for micropropagation. Murashige and Skoog ( ...

    Abstract An efficient indirect in vitro plant regeneration protocol for Dianthus caryophyllus L. cv. ‘Master’ was developed using leaf explants. This study revealed the morphogenetic potential of leaf explant as a source for micropropagation. Murashige and Skoog (MS) medium supplemented with 2.0 mg/l 2,4-dichlorophenoxyacetic acid and 0.5 mg/l Naphthalene acetic acid (NAA) resulted in maximum (94.44%) callus induction. MS medium supplemented with 1.5 mg/l thidiazuron, 0.25 mg/l Kinetin and 0.25 mg/l NAA was found to be highest for average shoot regeneration (80.56%), average number of shoots (6.01) and average shoot length (1.93 cm). For in vitro multiplication of shoots, MS medium supplemented with 2.0 mg/l Kinetin and 0.25 mg/l NAA was found to be the best which resulted in 14.64 average number of microshoots. It was observed that the half strength MS basal medium supplemented with 1.5 mg/l Indole-3-butyric acid and 0.02% activated charcoal showed maximum rooting (98.19%) with 9.60 average number of roots per microshoot having 4.24 cm root length. The in vitro rooted plantlets were hardened gradually and were successfully acclimatized under ex vitro conditions. The genetic variation in the in vitro raised plants was confirmed using DNA based markers [Random Amplified Polymorphic DNA and Inter Simple Sequence Repeats] for assessment of genetic stability of plants raised through indirect regeneration.
    Keywords 2,4-D ; activated carbon ; callus ; culture media ; Dianthus caryophyllus ; explants ; genetic stability ; genetic variation ; indole butyric acid ; kinetin ; leaves ; micropropagation ; microsatellite repeats ; organogenesis ; plantlets ; protocols ; random amplified polymorphic DNA technique ; rooting ; roots ; shoots ; thidiazuron
    Language English
    Dates of publication 2018-09
    Size p. 1147-1155.
    Publishing place Springer India
    Document type Article
    ZDB-ID 2707745-7
    ISSN 2250-1746 ; 0369-8211
    ISSN (online) 2250-1746
    ISSN 0369-8211
    DOI 10.1007/s40011-017-0851-2
    Database NAL-Catalogue (AGRICOLA)

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  8. Article: In-vitro propagation and genetic stability analysis of Swertia chirayita: An endangered high-value medicinal plant of Himalayas

    Kapoor, Bhuvnesh / Kanwar, Kamlesh / Sharma, Parul / Sharma, Rajnish / Thakur, Kalpna

    Medicinal Plants

    2019  Volume 11, Issue 3, Page(s) 328

    Language English
    Document type Article
    ZDB-ID 2881592-0
    ISSN 0975-4261
    Database Current Contents Nutrition, Environment, Agriculture

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    In stock of ZB MED Bonn / Germany

    Z 7969: Show issues

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  9. Article ; Online: SEM Description and Life History Traits of Indian Biotype of Acanthoscelides macrophthalmus (Schaeffer) (Coleoptera

    D.R. Thakur / Kalpna

    International Journal of Zoological Research, Vol 11, Iss 1, Pp 19-

    Bruchidae)

    2015  Volume 28

    Abstract: Many insect pests have crossed over the geographical boundaries and became cosmopolitan in distribution through anthropogenic migration and import and export of the comestible consignments. Acanthoscelides macrophthalmus (Schaeffer) (Coleoptera: ... ...

    Abstract Many insect pests have crossed over the geographical boundaries and became cosmopolitan in distribution through anthropogenic migration and import and export of the comestible consignments. Acanthoscelides macrophthalmus (Schaeffer) (Coleoptera: Chrysomelidae: Bruchidae) a recently reported well known pest of Leucaena leucocephala (Lamark) de Wit (Fabaceae) from Indian subcontinent has been studied for life history traits under laboratory conditions. Acanthoscelides macrophthalmus utilizes aphagously developing and developed seeds for oviposition and larval development. Eggs were laid either on the pod surface, over a seed, or directly on exposed seeds. Female laid an average of 38.3±8.01 eggs and produced 27.4±6.77 offsprings. Larvae grew and molted inside the host seed and adult bruchid emerged out after cutting a circular window in testa of the seed and pod as the case may be. Total development was completed in 41.6±7.18 days and newly emerged insects became sexually mature after one hour of emergence. All the four larval instars were studied Scanning Electron Microscopically (SEM) and head, antenna, clypeolabial complex, leg etc., were described separately and taxonomically important structure like setae, sensillae trichodea, microtrichia etc., beared by integumentry system were projected accordingly.
    Keywords pest ; life history ; Acanthoscelides macrophthalmus ; larval morphology ; Leucaena leucocephala ; Zoology ; QL1-991 ; Science ; Q
    Language English
    Publishing date 2015-01-01T00:00:00Z
    Publisher Academic Journals Inc.
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  10. Article: Direct Shoot Organogenesis from Seedling Derived Shoot Tip Explants of Endangered Medicinal Plant Saussurea costus (Falc.) Lipsch

    Sharma, Sneh / Sharma, Rajnish / Sharma, Parul / Thakur, Kalpna / Dutt, Bhupender

    Proceedings of the National Academy of Sciences, India, Section B: biological sciences. 2019 June, v. 89, no. 2

    2019  

    Abstract: An efficient direct in vitro plant regeneration protocol for Saussurea costus (kuth) was developed using shoot tip explants derived from in vitro grown seedlings to generate genetically uniform plants. Murashige and Skoog (MS) medium supplemented with 1 ... ...

    Abstract An efficient direct in vitro plant regeneration protocol for Saussurea costus (kuth) was developed using shoot tip explants derived from in vitro grown seedlings to generate genetically uniform plants. Murashige and Skoog (MS) medium supplemented with 1.14 μM thiadiazuron and 2.68 μM naphthalene acetic acid was found to be most optimal for highest for average shoot regeneration (73.33%) with maximum average number of shoots (11.4) and average shoot length (4.17 cm). For in vitro multiplication, shoots cultured on MS medium supplemented with 4.44 μM benzyl adenine, 2.32 μM kinetin and 1.44 μM gibberellic acid were found to grow best with 20.7 average shoot multiplication rate after 5–6 weeks. MS medium supplemented with 2.46 μM indole butyric acid was found to be the best medium for rooting of microshoots (77.78% rooting with 6.0 average number of roots and 3.07 cm average root length). The in vitro raised plantlets were successfully acclimatized under ex vitro conditions. The true to type nature of the in vitro raised plants was confirmed using DNA based ISSR markers for assessment of genetic stability of micropropagated plants. The standardized micropropagation protocol under present study may be tuned towards mass production of roots which are the most economical part of the plant. This would be useful as prerequisite for carrying out any genetic transformation studies while concentrating on any desirable trait in the same herb.
    Keywords Saussurea costus ; benzyladenine ; explants ; genetic stability ; genetic transformation ; gibberellic acid ; indole butyric acid ; kinetin ; medicinal plants ; micropropagation ; microsatellite repeats ; naphthaleneacetic acid ; organogenesis ; plantlets ; rooting ; roots ; seedlings ; shoot tips
    Language English
    Dates of publication 2019-06
    Size p. 755-764.
    Publishing place Springer India
    Document type Article
    ZDB-ID 2707745-7
    ISSN 2250-1746 ; 0369-8211
    ISSN (online) 2250-1746
    ISSN 0369-8211
    DOI 10.1007/s40011-018-0983-z
    Database NAL-Catalogue (AGRICOLA)

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