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  1. Book: Epithelial cell culture protocols

    Randell, Scott H. / Fulcher, M. Leslie

    (Methods in molecular biology ; 945 ; Springer protocols)

    2013  

    Author's details ed. by Scott H. Randall and M. Leslie Fulcher
    Series title Methods in molecular biology ; 945
    Springer protocols
    Collection
    Keywords Cell Culture Techniques / methods ; Epithelial Cells ; Epithelzelle ; Zellkultur
    Subject Zelle ; Zellzüchtung
    Language English
    Size 469 S. : Ill., graph. Darst.
    Edition 2. ed.
    Publisher Humana Press
    Publishing place New York u.a.
    Publishing country United States
    Document type Book
    HBZ-ID HT017443532
    ISBN 978-1-62703-124-0 ; 1-62703-124-3 ; 9781627031257 ; 1627031251
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

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    2012 A 5775 order for loan Magazin

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  2. Article ; Online: The Big Impact of Small Airway pH.

    Okuda, Kenichi / Randell, Scott H / Birket, Susan E

    American journal of respiratory cell and molecular biology

    2021  Volume 65, Issue 2, Page(s) 123–125

    MeSH term(s) Hydrogen-Ion Concentration ; Respiratory System
    Language English
    Publishing date 2021-04-08
    Publishing country United States
    Document type Editorial ; Comment
    ZDB-ID 1025960-0
    ISSN 1535-4989 ; 1044-1549
    ISSN (online) 1535-4989
    ISSN 1044-1549
    DOI 10.1165/rcmb.2021-0070ED
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Evaluation of chronic cigarette smoke exposure in human bronchial epithelial cultures.

    Tyrrell, Jean / Ghosh, Arunava / Manzo, Nicholas D / Randell, Scott H / Tarran, Robert

    Journal of applied toxicology : JAT

    2023  Volume 43, Issue 6, Page(s) 862–873

    Abstract: Cigarette smoke (CS) exposure induces both cytotoxicity and inflammation, and often causes COPD, a growing cause of morbidity and mortality. CS also inhibits the CFTR ... ...

    Abstract Cigarette smoke (CS) exposure induces both cytotoxicity and inflammation, and often causes COPD, a growing cause of morbidity and mortality. CS also inhibits the CFTR Cl
    MeSH term(s) Humans ; Cystic Fibrosis Transmembrane Conductance Regulator ; Cigarette Smoking ; Bronchi ; Nicotiana/toxicity ; Inflammation ; Epithelial Cells
    Chemical Substances Cystic Fibrosis Transmembrane Conductance Regulator (126880-72-6)
    Language English
    Publishing date 2023-01-13
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, P.H.S.
    ZDB-ID 604625-3
    ISSN 1099-1263 ; 0260-437X
    ISSN (online) 1099-1263
    ISSN 0260-437X
    DOI 10.1002/jat.4430
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  4. Article ; Online: Air-Liquid interface cultures to model drug delivery through the mucociliary epithelial barrier.

    Lee, Rhianna E / Reidel, Boris / Nelson, Mark R / Macdonald, Jade K / Kesimer, Mehmet / Randell, Scott H

    Advanced drug delivery reviews

    2023  Volume 198, Page(s) 114866

    Abstract: Epithelial cells from mucociliary portions of the airways can be readily grown and expanded in vitro. When grown on a porous membrane at an air-liquid interface (ALI) the cells form a confluent, electrically resistive barrier separating the apical and ... ...

    Abstract Epithelial cells from mucociliary portions of the airways can be readily grown and expanded in vitro. When grown on a porous membrane at an air-liquid interface (ALI) the cells form a confluent, electrically resistive barrier separating the apical and basolateral compartments. ALI cultures replicate key morphological, molecular and functional features of the in vivo epithelium, including mucus secretion and mucociliary transport. Apical secretions contain secreted gel-forming mucins, shed cell-associated tethered mucins, and hundreds of additional molecules involved in host defense and homeostasis. The respiratory epithelial cell ALI model is a time-proven workhorse that has been employed in various studies elucidating the structure and function of the mucociliary apparatus and disease pathogenesis. It serves as a critical milestone test for small molecule and genetic therapies targeting airway diseases. To fully exploit the potential of this important tool, numerous technical variables must be thoughtfully considered and carefully executed.
    MeSH term(s) Humans ; Cells, Cultured ; Mucins ; Epithelial Cells ; Epithelium ; Mucociliary Clearance
    Chemical Substances Mucins
    Language English
    Publishing date 2023-05-15
    Publishing country Netherlands
    Document type Journal Article ; Review ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 639113-8
    ISSN 1872-8294 ; 0169-409X
    ISSN (online) 1872-8294
    ISSN 0169-409X
    DOI 10.1016/j.addr.2023.114866
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Author Correction: Unsupervised discovery of tissue architecture in multiplexed imaging.

    Kim, Junbum / Rustam, Samir / Mosquera, Juan Miguel / Randell, Scott H / Shaykhiev, Renat / Rendeiro, André F / Elemento, Olivier

    Nature methods

    2022  Volume 19, Issue 12, Page(s) 1662

    Language English
    Publishing date 2022-11-22
    Publishing country United States
    Document type Published Erratum
    ZDB-ID 2169522-2
    ISSN 1548-7105 ; 1548-7091
    ISSN (online) 1548-7105
    ISSN 1548-7091
    DOI 10.1038/s41592-022-01717-7
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: A Slippery Cause of a Slimy Problem: Mucin Induction by an Esterified Lipid.

    Randell, Scott H / Zeldin, Darryl C

    American journal of respiratory cell and molecular biology

    2017  Volume 57, Issue 6, Page(s) 633–634

    MeSH term(s) Animals ; Asthma/metabolism ; Asthma/pathology ; Bronchitis, Chronic/metabolism ; Bronchitis, Chronic/pathology ; Cystic Fibrosis/metabolism ; Cystic Fibrosis/pathology ; Goblet Cells/metabolism ; Goblet Cells/pathology ; Humans ; Hydroxyeicosatetraenoic Acids/metabolism ; Idiopathic Pulmonary Fibrosis/metabolism ; Idiopathic Pulmonary Fibrosis/pathology ; Mucins/biosynthesis
    Chemical Substances Hydroxyeicosatetraenoic Acids ; Mucins ; 15-hydroxy-5,8,11,13-eicosatetraenoic acid (73945-47-8)
    Language English
    Publishing date 2017
    Publishing country United States
    Document type Editorial
    ZDB-ID 1025960-0
    ISSN 1535-4989 ; 1044-1549
    ISSN (online) 1535-4989
    ISSN 1044-1549
    DOI 10.1165/rcmb.2017-0275ED
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: Challenges Facing Airway Epithelial Cell-Based Therapy for Cystic Fibrosis.

    Berical, Andrew / Lee, Rhianna E / Randell, Scott H / Hawkins, Finn

    Frontiers in pharmacology

    2019  Volume 10, Page(s) 74

    Abstract: Mutations in the cystic fibrosis transmembrane conductance regulator ( ...

    Abstract Mutations in the cystic fibrosis transmembrane conductance regulator (
    Language English
    Publishing date 2019-02-08
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2587355-6
    ISSN 1663-9812
    ISSN 1663-9812
    DOI 10.3389/fphar.2019.00074
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  8. Article ; Online: Unsupervised discovery of tissue architecture in multiplexed imaging.

    Kim, Junbum / Rustam, Samir / Mosquera, Juan Miguel / Randell, Scott H / Shaykhiev, Renat / Rendeiro, André F / Elemento, Olivier

    Nature methods

    2022  Volume 19, Issue 12, Page(s) 1653–1661

    Abstract: Multiplexed imaging and spatial transcriptomics enable highly resolved spatial characterization of cellular phenotypes, but still largely depend on laborious manual annotation to understand higher-order patterns of tissue organization. As a result, ... ...

    Abstract Multiplexed imaging and spatial transcriptomics enable highly resolved spatial characterization of cellular phenotypes, but still largely depend on laborious manual annotation to understand higher-order patterns of tissue organization. As a result, higher-order patterns of tissue organization are poorly understood and not systematically connected to disease pathology or clinical outcomes. To address this gap, we developed an approach called UTAG to identify and quantify microanatomical tissue structures in multiplexed images without human intervention. Our method combines information on cellular phenotypes with the physical proximity of cells to accurately identify organ-specific microanatomical domains in healthy and diseased tissue. We apply our method to various types of images across healthy and disease states to show that it can consistently detect higher-level architectures in human tissues, quantify structural differences between healthy and diseased tissue, and reveal tissue organization patterns at the organ scale.
    MeSH term(s) Humans ; Diagnostic Imaging ; Transcriptome
    Language English
    Publishing date 2022-10-31
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2169522-2
    ISSN 1548-7105 ; 1548-7091
    ISSN (online) 1548-7105
    ISSN 1548-7091
    DOI 10.1038/s41592-022-01657-2
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  9. Article ; Online: Burn Pit Smoke Condensate-Mediated Toxicity in Human Airway Epithelial Cells.

    Ghosh, Arunava / Payton, Alexis / Gallant, Samuel C / Rogers, Keith L / Mascenik, Teresa / Hickman, Elise / Love, Charlotte A / Schichlein, Kevin D / Smyth, Timothy R / Kim, Yong Ho / Rager, Julia E / Gilmour, M Ian / Randell, Scott H / Jaspers, Ilona

    Chemical research in toxicology

    2024  

    Abstract: Burn pits are a method of open-air waste management that was common during military operations in Iraq, Afghanistan, and other regions in Southwest Asia. Veterans returning from deployment have reported respiratory symptoms, potentially from exposure to ... ...

    Abstract Burn pits are a method of open-air waste management that was common during military operations in Iraq, Afghanistan, and other regions in Southwest Asia. Veterans returning from deployment have reported respiratory symptoms, potentially from exposure to burn pit smoke, yet comprehensive assessment of such exposure on pulmonary health is lacking. We have previously shown that exposure to condensates from burn pit smoke emissions causes inflammation and cytotoxicity in mice. In this study, we explored the effects of burn pit smoke condensates on human airway epithelial cells (HAECs) to understand their impact on cellular targets in the human lung. HAECs were cultured at the air-liquid interface (ALI) and exposed to burn pit waste smoke condensates (plywood, cardboard, plastic, mixed, and mixed with diesel) generated under smoldering and flaming conditions. Cytotoxicity was evaluated by measuring transepithelial electrical resistance (TEER) and lactate dehydrogenase (LDH) release; toxicity scores (TSs) were quantified for each exposure. Pro-inflammatory cytokine release and modulation of gene expression were examined for cardboard and plastic condensate exposures. Burn pit smoke condensates generated under flaming conditions affected cell viability, with flaming mixed waste and plywood exhibiting the highest toxicity scores. Cardboard and plastic smoke condensates modulated cytokine secretion, with GM-CSF and IL-1β altered in more than one exposure group. Gene expression of detoxifying enzymes (
    Language English
    Publishing date 2024-04-23
    Publishing country United States
    Document type Journal Article
    ZDB-ID 639353-6
    ISSN 1520-5010 ; 0893-228X
    ISSN (online) 1520-5010
    ISSN 0893-228X
    DOI 10.1021/acs.chemrestox.4c00064
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  10. Article ; Online: Bik promotes proteasomal degradation to control low-grade inflammation.

    Mebratu, Yohannes A / Jones, Jane T / Liu, Congjian / Negasi, Zerihun H / Rahman, Mizanur / Rojas-Quintero, Joselyn / O'Connor, George T / Gao, Wei / Dupuis, Josée / Cho, Michael H / Litonjua, Augusto A / Randell, Scott / Tesfaigzi, Yohannes

    The Journal of clinical investigation

    2023  Volume 134, Issue 4

    Abstract: Although chronic low-grade inflammation does not cause immediate clinical symptoms, over the longer term, it can enhance other insults or age-dependent damage to organ systems and thereby contribute to age-related disorders, such as respiratory disorders, ...

    Abstract Although chronic low-grade inflammation does not cause immediate clinical symptoms, over the longer term, it can enhance other insults or age-dependent damage to organ systems and thereby contribute to age-related disorders, such as respiratory disorders, heart disease, metabolic disorders, autoimmunity, and cancer. However, the molecular mechanisms governing low-level inflammation are largely unknown. We discovered that Bcl-2-interacting killer (Bik) deficiency causes low-level inflammation even at baseline and the development of spontaneous emphysema in female but not male mice. Similarly, a single nucleotide polymorphism that reduced Bik levels was associated with increased inflammation and enhanced decline in lung function in humans. Transgenic expression of Bik in the airways of Bik-deficient mice inhibited allergen- or LPS-induced lung inflammation and reversed emphysema in female mice. Bik deficiency increased nuclear but not cytosolic p65 levels because Bik, by modifying the BH4 domain of Bcl-2, interacted with regulatory particle non-ATPase 1 (RPN1) and RPN2 and enhanced proteasomal degradation of nuclear proteins. Bik deficiency increased inflammation primarily in females because Bcl-2 and Bik levels were reduced in lung tissues and airway cells of female compared with male mice. Therefore, controlling low-grade inflammation by modifying the unappreciated role of Bik and Bcl-2 in facilitating proteasomal degradation of nuclear proteins may be crucial in treating chronic age-related diseases.
    MeSH term(s) Male ; Animals ; Female ; Humans ; Mice ; Apoptosis ; Mitochondrial Proteins ; Apoptosis Regulatory Proteins ; Proto-Oncogene Proteins c-bcl-2 ; Inflammation/genetics ; Nuclear Proteins ; Emphysema ; Hexosyltransferases ; Proteasome Endopeptidase Complex/genetics
    Chemical Substances Mitochondrial Proteins ; Apoptosis Regulatory Proteins ; Proto-Oncogene Proteins c-bcl-2 ; Nuclear Proteins ; RPN2 protein, human (EC 2.4.99.18) ; Hexosyltransferases (EC 2.4.1.-) ; Proteasome Endopeptidase Complex (EC 3.4.25.1) ; Bik protein, mouse
    Language English
    Publishing date 2023-12-19
    Publishing country United States
    Document type Journal Article
    ZDB-ID 3067-3
    ISSN 1558-8238 ; 0021-9738
    ISSN (online) 1558-8238
    ISSN 0021-9738
    DOI 10.1172/JCI170594
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