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Article ; Online: Counting of enzymatically amplified affinity reactions in hydrogel particle-templated drops.

Wang, Yilian / Shah, Vishwesh / Lu, Angela / Pachler, Ella / Cheng, Brian / Di Carlo, Dino

2021 Volume 21, Issue 18, Page(s) 3438–3448

Abstract: Counting of numerous compartmentalized enzymatic reactions underlies quantitative and high sensitivity immunodiagnostic assays. However, digital enzyme-linked immunosorbent assays (ELISA) require specialized instruments which have slowed adoption in ... ...

Abstract	Counting of numerous compartmentalized enzymatic reactions underlies quantitative and high sensitivity immunodiagnostic assays. However, digital enzyme-linked immunosorbent assays (ELISA) require specialized instruments which have slowed adoption in research and clinical labs. We present a lab-on-a-particle solution to digital counting of thousands of single enzymatic reactions. Hydrogel particles are used to bind enzymes and template the formation of droplets that compartmentalize reactions with simple pipetting steps. These hydrogel particles can be made at a high throughput, stored, and used during the assay to create ∼500 000 compartments within 2 minutes. These particles can also be dried and rehydrated with sample, amplifying the sensitivity of the assay by driving affinity interactions on the hydrogel surface. We demonstrate digital counting of β-galactosidase enzyme at a femtomolar detection limit with a dynamic range of 3 orders of magnitude using standard benchtop equipment and experiment techniques. This approach can faciliate the development of digital ELISAs with reduced need for specialized microfluidic devices, instruments, or imaging systems.
MeSH term(s)	Biological Assay ; Enzyme-Linked Immunosorbent Assay ; Hydrogels ; Lab-On-A-Chip Devices
Chemical Substances	Hydrogels
Language	English
Publishing date	2021-09-14
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	2056646-3
ISSN	1473-0189 ; 1473-0197
ISSN (online)	1473-0189
ISSN	1473-0197
DOI	10.1039/d1lc00344e
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Effects of impurities H2S and N2 on CO2 migration and dissolution in sedimentary geothermal reservoirs

Yu, Ying / Li, Yilian / Cheng, Fei / Yang, Guodong / Ma, Xin / Cao, Wei

Journal of hydrology. 2021 Dec., v. 603

2021

Abstract: CO₂ geological storage is a key technology for reducing greenhouse gas emissions. But merely injecting CO₂ into sedimentary strata has high costs and no direct economic benefit. A more cost-effective and environment-friendly way is storing and utilizing ... ...

Abstract	CO₂ geological storage is a key technology for reducing greenhouse gas emissions. But merely injecting CO₂ into sedimentary strata has high costs and no direct economic benefit. A more cost-effective and environment-friendly way is storing and utilizing CO₂ in deep sedimentary reservoirs for heat extraction. However, the effects of ubiquitous impurities on the sequestration safety and heat extraction performance of CO₂ in high-temperature reservoirs, are unclear. In this study, we have performed numerical simulations of multiphase flow, solute transport, and heat conduction at reservoir temperature 50–250 °C, to reveal the effects of two common impurities (i.e., H₂S and N₂) on the migration, dissolution and thermal absorption of CO₂ in sedimentary geothermal reservoirs. It is found that, for higher reservoir temperature, the impurity N₂ leads to smaller promotion on the migration of gas phase, but retains the acceleration effect on the cooling in reservoir. Above 150 °C, the deviations on the gas phase migration velocity and the CO₂ dissolution efficiency caused by 10 mol% N₂ are less than 12%. Meanwhile, the 10 mol% N₂ can increase the total heat absorbed from reservoir by about 40% at least. It means that N₂ has the potential to benefit a CO₂ plume geothermal system significantly without distinct risks. Besides, it is also found that the CO₂ dissolution efficiency can be increased and decreased by H₂S and N₂ through partitioning phenomenon, respectively.
Keywords	absorption ; carbon dioxide ; cooling ; cost effectiveness ; financial economics ; greenhouse gases ; heat transfer ; hydrology ; solutes ; temperature
Language	English
Dates of publication	2021-12
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	1473173-3
ISSN	1879-2707 ; 0022-1694
ISSN (online)	1879-2707
ISSN	0022-1694
DOI	10.1016/j.jhydrol.2021.126959
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Effects of Different Methods of Air Disinfection of Computed Tomography Rooms Dedicated to COVID-19 Cases

Yilian Cheng / Jing Hu / Hui Chen / Liu Wu / Jianmei Liao / Lin Cheng

BioMed Research International, Vol

2020 Volume 2020

Abstract: Objective. To monitor the number of bacterial colonies in the air of computed tomography (CT) room for COVID-19 using different disinfection methods and to identify the most effective method for disinfection and protection of equipment. Methods. Three ... ...

Abstract	Objective. To monitor the number of bacterial colonies in the air of computed tomography (CT) room for COVID-19 using different disinfection methods and to identify the most effective method for disinfection and protection of equipment. Methods. Three methods for disinfection using ultraviolet germicidal irradiation (group A), plasma circulation air sterilizer (group B), and ultraviolet germicidal irradiation plus plasma circulation air sterilizer (group C) were utilized to sanitize the air in the CT room dedicated to COVID-19 cases. Single-factor ANOVA was used to evaluate and compare the disinfection effect of the three air disinfection methods; an air microbial sampler was used to sample and measure the number of bacteria in the air of the machine room. Results. The number of bacteria in the air immediately after disinfection was significantly lower than before disinfection (p<0.01). All three disinfection methods met the disinfection requirement. No significant differences in the number of air bacteria in the machine room immediately after disinfection were observed among the three methods (p>0.05). In addition, the effect of disinfection after 2 h was compared, and the number of bacteria in group C after 2 h was significantly lower than that in group A and group B. Conclusions. All three disinfection methods have significant disinfection effects. In addition, using ultraviolet disinfection lamps combined with a plasma air disinfection machine to sterilize the air in CT machine room has the best disinfection effect for the longest duration. Therefore, we recommend the combined disinfection method (ultraviolet disinfection lamps plus plasma air disinfection), as well as formulating relevant disinfection management norms, which should thus be the method to use during pandemics.
Keywords	Medicine ; R
Subject code	630
Language	English
Publishing date	2020-01-01T00:00:00Z
Publisher	Hindawi Limited
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Effects of Different Methods of Air Disinfection of Computed Tomography Rooms Dedicated to COVID-19 Cases.

Cheng, Yilian / Hu, Jing / Chen, Hui / Wu, Liu / Liao, Jianmei / Cheng, Lin

BioMed research international

2020 Volume 2020, Page(s) 5302910

Abstract: Objective: To monitor the number of bacterial colonies in the air of computed tomography (CT) room for COVID-19 using different disinfection methods and to identify the most effective method for disinfection and protection of equipment.: Methods: ... ...

Abstract	Objective: To monitor the number of bacterial colonies in the air of computed tomography (CT) room for COVID-19 using different disinfection methods and to identify the most effective method for disinfection and protection of equipment. Methods: Three methods for disinfection using ultraviolet germicidal irradiation (group A), plasma circulation air sterilizer (group B), and ultraviolet germicidal irradiation plus plasma circulation air sterilizer (group C) were utilized to sanitize the air in the CT room dedicated to COVID-19 cases. Single-factor ANOVA was used to evaluate and compare the disinfection effect of the three air disinfection methods; an air microbial sampler was used to sample and measure the number of bacteria in the air of the machine room. Results: The number of bacteria in the air immediately after disinfection was significantly lower than before disinfection ( Conclusions: All three disinfection methods have significant disinfection effects. In addition, using ultraviolet disinfection lamps combined with a plasma air disinfection machine to sterilize the air in CT machine room has the best disinfection effect for the longest duration. Therefore, we recommend the combined disinfection method (ultraviolet disinfection lamps plus plasma air disinfection), as well as formulating relevant disinfection management norms, which should thus be the method to use during pandemics.
MeSH term(s)	COVID-19/epidemiology ; COVID-19/prevention & control ; Disinfection ; Humans ; Infection Control ; SARS-CoV-2 ; Tomography, X-Ray Computed
Language	English
Publishing date	2020-11-22
Publishing country	United States
Document type	Journal Article
ZDB-ID	2698540-8
ISSN	2314-6141 ; 2314-6133
ISSN (online)	2314-6141
ISSN	2314-6133
DOI	10.1155/2020/5302910
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Effects of short-chain fatty acids in inhibiting HDAC and activating p38 MAPK are critical for promoting B10 cell generation and function.

Zou, Fagui / Qiu, Yi / Huang, Yilian / Zou, Hang / Cheng, Xiao / Niu, Qingru / Luo, Aoxiang / Sun, Jianbo

Cell death & disease

2021 Volume 12, Issue 6, Page(s) 582

Abstract: B10 cells are regulatory B cells capable of producing IL-10 for maintaining immune homeostasis. Dysregulation of B10 cells occurs in autoimmune and inflammatory diseases. Modulation or adoptive transfer of B10 cells is a promising therapeutic strategy. ... ...

Abstract	B10 cells are regulatory B cells capable of producing IL-10 for maintaining immune homeostasis. Dysregulation of B10 cells occurs in autoimmune and inflammatory diseases. Modulation or adoptive transfer of B10 cells is a promising therapeutic strategy. The short-chain fatty acids (SCFAs), the metabolites of microbiota, play a critical role in maintaining immune homeostasis and are the potential drugs for the modulation of B10 cells. It is not clear whether and how SCFAs upregulate the frequency of B10 cells. Here, we found that SCFAs could promote murine and human B10 cell generation in vitro. Upregulation of B10 cells by butyrate or pentanoate was also observed in either healthy mice, mice with dextran sodium sulfate (DSS)-induced colitis, or mice with collagen-induced arthritis. Moreover, SCFA treatment could ameliorate clinical scores of colitis and arthritis. Adoptive transfer of B cells pretreated with butyrate showed more alleviation of DSS-induced colitis than those without butyrate. A further study demonstrates that SCFAs upregulate B10 cells in a manner dependent on their histone deacetylase (HDAC) inhibitory activity and independent of the G-protein-coupled receptor pathway. Transcriptomic analysis indicated that the MAPK signaling pathway was enriched in B10 cells treated with butyrate. A study with inhibitors of ERK, JNK, and p38 MAPK demonstrated that activating p38 MAPK by butyrate is critical for the upregulation of B10 cells. Moreover, HDAC inhibitor has similar effects on B10 cells. Our study sheds light on the mechanism underlying B10 cell differentiation and function and provides a potential therapeutic strategy with SCFAs and HDAC inhibitors for inflammation and autoimmune diseases.
MeSH term(s)	Animals ; Cell Differentiation ; Fatty Acids, Volatile/metabolism ; Histone Deacetylase Inhibitors/pharmacology ; Histone Deacetylase Inhibitors/therapeutic use ; Humans ; Mice ; p38 Mitogen-Activated Protein Kinases/metabolism
Chemical Substances	Fatty Acids, Volatile ; Histone Deacetylase Inhibitors ; p38 Mitogen-Activated Protein Kinases (EC 2.7.11.24)
Language	English
Publishing date	2021-06-07
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2541626-1
ISSN	2041-4889 ; 2041-4889
ISSN (online)	2041-4889
ISSN	2041-4889
DOI	10.1038/s41419-021-03880-9
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Uncaria Rhynchophylla attenuates angiotensin Ⅱ-induced myocardial fibrosis via suppression of the RhoA/ROCK1 pathway.

Xie, Lingling / Wang, Tianyi / Lin, Shan / Lu, Zhuqing / Wang, Yilian / Shen, Zhiqing / Cheng, Ying / Shen, Aling / Peng, Jun / Chu, Jianfeng

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

2021 Volume 146, Page(s) 112607

Abstract: Uncaria rhynchophylla (UR), a traditional Chinese medicine, has been proven effective in treating hypertensive patients in China. However, the mechanisms of action of UR in reducing hypertension and myocardial fibrosis are still unclear. The purpose of ... ...

Abstract	Uncaria rhynchophylla (UR), a traditional Chinese medicine, has been proven effective in treating hypertensive patients in China. However, the mechanisms of action of UR in reducing hypertension and myocardial fibrosis are still unclear. The purpose of this study was to explore the role of UR in an angiotensin Ⅱ (Ang Ⅱ) induced mouse model. The mice were randomly divided into 5 groups and infused with Ang Ⅱ (500 ng/kg/min) or saline, then administered UR (0.78, 1.56 or 3.12 g/kg/d) or saline for 4 weeks. UR treatment significantly attenuated the elevation of blood pressure caused by Ang Ⅱ. It enhanced myocardial function and attenuated the increase in the heart weight index and the pathological changes in the Ang Ⅱ-induced hypertensive mice. Furthermore, UR treatment inhibited cardiac fibrosis and significantly down-regulated collagen I, collagen Ⅲ, and α-SMA protein expression in cardiac tissues. UR also attenuated the expression of RhoA, ROCK1, CTGF, and TGF-β1. In cultured cardiac fibroblasts stimulated with Ang Ⅱ, UR significantly down-regulated the expression of Collagen I, Collagen III, RhoA, ROCK1, and α-SMA. In summary, UR can significantly attenuate Ang Ⅱ-induced hypertension and cardiac fibrosis, partly via suppression of the RhoA/ROCK1 signaling pathway.
MeSH term(s)	Angiotensin II/pharmacology ; Animals ; Blood Pressure/drug effects ; Cardiomyopathies/prevention & control ; Hypertension/drug therapy ; Mice ; Mice, Inbred C57BL ; Myocardium/pathology ; Signal Transduction ; Uncaria/metabolism ; rho-Associated Kinases ; rhoA GTP-Binding Protein
Chemical Substances	Angiotensin II (11128-99-7) ; RHOA protein, human (124671-05-2) ; ROCK1 protein, human (EC 2.7.11.1) ; rho-Associated Kinases (EC 2.7.11.1) ; rhoA GTP-Binding Protein (EC 3.6.5.2)
Language	English
Publishing date	2021-12-30
Publishing country	France
Document type	Journal Article
ZDB-ID	392415-4
ISSN	1950-6007 ; 0753-3322 ; 0300-0893
ISSN (online)	1950-6007
ISSN	0753-3322 ; 0300-0893
DOI	10.1016/j.biopha.2021.112607
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Lung Ultrasound Score as a Predictor of Mortality in Patients With COVID-19.

Sun, Zhenxing / Zhang, Ziming / Liu, Jie / Song, Yue / Qiao, Shi / Duan, Yilian / Cao, Haiyan / Xie, Yuji / Wang, Rui / Zhang, Wen / You, Manjie / Yu, Cheng / Ji, Li / Cao, Chunyan / Wang, Jing / Yang, Yali / Lv, Qing / Wang, Hongbo / Gu, Haotian /

Xie, Mingxing

Frontiers in cardiovascular medicine

2021 Volume 8, Page(s) 633539

Abstract: Background: ...

Abstract	Background:
Language	English
Publishing date	2021-05-25
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2781496-8
ISSN	2297-055X
ISSN	2297-055X
DOI	10.3389/fcvm.2021.633539
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Problems and solutions of personal protective equipment doffing in COVID-19

Cheng Lin / Chen Lei / Xiao Li / Zhang Jianping / Cheng Yilian / Zhou Lian / Peng Yu / Liu Lei

Open Medicine, Vol 15, Iss 1, Pp 605-

2020 Volume 612

Abstract: The progress of the coronavirus disease 2019 (COVID-19) pandemic is still severe. While everyone has been striving very hard to combat the pandemic, some healthcare professionals have shown varying degrees of infection symptoms and even died. The Chinese ...

Abstract	The progress of the coronavirus disease 2019 (COVID-19) pandemic is still severe. While everyone has been striving very hard to combat the pandemic, some healthcare professionals have shown varying degrees of infection symptoms and even died. The Chinese Army Medical Aid Team arrived in Wuhan on January 25, 2020, and quickly entered the battle against the pandemic after a short and rigorous training. As one of the earliest medical teams to enter the pandemic area, researchers have been exploring effective infection control measures that are currently in practice. Through observation and research, it has been noticed that layers of protective equipment have a hidden danger of infection while protecting the safety of the personnel. The members of each medical team have typically focused on the standard use of personal protective equipment (PPE). However, after a long period of intensive diagnosis and treatment in clinics and due to physical exertion and tiredness, problems such as improper operation are prone to occur during the tedious PPE doffing, thereby producing a relatively high risk of infection. This study primarily analyzes PPE doffing procedures, existing problems, and measures for improvement to explore methods to improve PPE donning and doffing and reduce the risk of infection.
Keywords	coronavirus disease 2019 (covid-19) ; origin of the pandemic ; occupational exposure ; Medicine ; R ; covid19
Subject code	650
Language	English
Publishing date	2020-07-01T00:00:00Z
Publisher	De Gruyter
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Kondo Holes in the Two-Dimensional Itinerant Ising Ferromagnet Fe

Zhao, Mengting / Chen, Bin-Bin / Xi, Yilian / Zhao, Yanyan / Xu, Hang / Zhang, Hongrun / Cheng, Ningyan / Feng, Haifeng / Zhuang, Jincheng / Pan, Feng / Xu, Xun / Hao, Weichang / Li, Wei / Zhou, Si / Dou, Shi Xue / Du, Yi

Nano letters

2021

Abstract: Heavy Fermion (HF) states emerge in correlated quantum materials due to the intriguing interplay between localized magnetic moments and itinerant electrons but rarely appear in 3d-electron systems due to high itinerancy of d-electrons. Here, an anomalous ...

Abstract	Heavy Fermion (HF) states emerge in correlated quantum materials due to the intriguing interplay between localized magnetic moments and itinerant electrons but rarely appear in 3d-electron systems due to high itinerancy of d-electrons. Here, an anomalous enhancement of Kondo screening is observed at the Kondo hole of local Fe vacancies in Fe
Language	English
Publishing date	2021-07-19
Publishing country	United States
Document type	Journal Article
ISSN	1530-6992
ISSN (online)	1530-6992
DOI	10.1021/acs.nanolett.1c01661
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Problems and solutions of personal protective equipment doffing in COVID-19.

Cheng, Lin / Chen, Lei / Xiao, Li / Zhang, Jianping / Cheng, Yilian / Zhou, Lian / Peng, Yu / Liu, Lei

Open medicine (Warsaw, Poland)

2020 Volume 15, Issue 1, Page(s) 605–612

Abstract	The progress of the coronavirus disease 2019 (COVID-19) pandemic is still severe. While everyone has been striving very hard to combat the pandemic, some healthcare professionals have shown varying degrees of infection symptoms and even died. The Chinese Army Medical Aid Team arrived in Wuhan on January 25, 2020, and quickly entered the battle against the pandemic after a short and rigorous training. As one of the earliest medical teams to enter the pandemic area, researchers have been exploring effective infection control measures that are currently in practice. Through observation and research, it has been noticed that layers of protective equipment have a hidden danger of infection while protecting the safety of the personnel. The members of each medical team have typically focused on the standard use of personal protective equipment (PPE). However, after a long period of intensive diagnosis and treatment in clinics and due to physical exertion and tiredness, problems such as improper operation are prone to occur during the tedious PPE doffing, thereby producing a relatively high risk of infection. This study primarily analyzes PPE doffing procedures, existing problems, and measures for improvement to explore methods to improve PPE donning and doffing and reduce the risk of infection.
Language	English
Publishing date	2020-07-02
Publishing country	Poland
Document type	Journal Article
ZDB-ID	2829380-0
ISSN	2391-5463
ISSN	2391-5463
DOI	10.1515/med-2020-0172
Database	MEDical Literature Analysis and Retrieval System OnLINE

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