Article: 3D dynamic cultures of HGSOC organoids to model innovative and standard therapies.
Frontiers in bioengineering and biotechnology
2023 Volume 11, Page(s) 1135374
Abstract: High-grade serous ovarian cancer (HGSOC) needs new technologies for improving cancer diagnosis and therapy. It is a fatal disease with few options for the patients. In this context, dynamic culture systems coupling with patient-derived cancer 3D ... ...
Abstract | High-grade serous ovarian cancer (HGSOC) needs new technologies for improving cancer diagnosis and therapy. It is a fatal disease with few options for the patients. In this context, dynamic culture systems coupling with patient-derived cancer 3D microstructures could offer a new opportunity for exploring novel therapeutic approaches. In this study, we optimized a passive microfluidic platform with 3D cancer organoids, which allows a standardized approach among different patients, a minimum requirement of samples, multiple interrogations of biological events, and a rapid response. The passive flow was optimized to improve the growth of cancer organoids, avoiding the disruption of the extracellular matrix (ECM). Under optimized conditions of the OrganoFlow (tilting angle of 15° and an interval of rocking every 8 min), the cancer organoids grow faster than when they are in static conditions and the number of dead cells is reduced over time. To calculate the |
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Language | English |
Publishing date | 2023-04-18 |
Publishing country | Switzerland |
Document type | Journal Article |
ZDB-ID | 2719493-0 |
ISSN | 2296-4185 |
ISSN | 2296-4185 |
DOI | 10.3389/fbioe.2023.1135374 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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