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Article ; Online: Analysis of life expectancy in Russian regions

Kapitanova Olga / Kuznetsov Iurii / Semenov Alexey / Kisova Veronika / Koneva Irina

E3S Web of Conferences, Vol 380, p

2023 Volume 01038

Abstract: This study deals with the analysis of the factors that determine life expectancy in Russia at the macro level. Among the available statistical indicators, those were selected that can be considered as a macroeconomic alternative to the basic ... ...

Abstract	This study deals with the analysis of the factors that determine life expectancy in Russia at the macro level. Among the available statistical indicators, those were selected that can be considered as a macroeconomic alternative to the basic microeconomic determinants affecting the life expectancy of an individual. As a result of the regression analysis, significant indicators were identified, which included the marriage rate, the unemployment rate, the number of students, the number of hospital beds, the gross regional product, budget expenditures on health care, and the region’s subtropical climate. A cluster analysis of the regions was also carried out for these indicators, with the exception of the last one, and it was shown that the regions are distributed into 5 clusters. The cluster, which includes Moscow and St. Petersburg, where there is a high life expectancy due to the metropolitan area of these regions and good medicine, is singled out separately. The regions of the North Caucasus are also characterized by high life expectancy, but here it is due to completely different reasons: religious and climatic features. The Tyumen, Magadan, Sakhalin regions and the Chukotka Autonomous Okrug are united into a separate cluster. These regions are characterized by the highest GRP, but low life expectancy and the lowest number of students.
Keywords	Environmental sciences ; GE1-350
Subject code	300
Language	English
Publishing date	2023-01-01T00:00:00Z
Publisher	EDP Sciences
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Next generation CANCAN focusing for remote stimulation by nanosecond electric pulses.

Pakhomov, Andrei G / Gudvangen, Emily / Mangalanathan, Uma / Kondratiev, Oleg / Redondo, Luis / Semenov, Iurii

Bioelectrochemistry (Amsterdam, Netherlands)

2023 Volume 152, Page(s) 108437

Abstract: Focusing electric pulse effects away from electrodes is a challenge because the electric field weakens with distance. Previously we introduced a remote focusing method based on bipolar cancellation, a phenomenon of low efficiency of bipolar nanosecond ... ...

Abstract	Focusing electric pulse effects away from electrodes is a challenge because the electric field weakens with distance. Previously we introduced a remote focusing method based on bipolar cancellation, a phenomenon of low efficiency of bipolar nanosecond electric pulses (nsEP). Superpositioning two bipolar nsEP into a unipolar pulse canceled bipolar cancellation ("CANCAN" effect), enhancing bioeffects at a distance despite the electric field weakening. Here, we introduce the next generation (NG) CANCAN focusing with unipolar nsEP packets designed to produce bipolar waveforms near electrodes (suppressing electroporation) but not at the remote target. NG-CANCAN was tested in CHO cell monolayers using a quadrupole electrode array and labeling electroporated cells with YO-PRO-1 dye. We routinely achieved 1.5-2 times stronger electroporation in the center of the quadrupole than near electrodes, despite a 3-4-fold field attenuation. With the array lifted 1-2 mm above the monolayer (imitating a 3D treatment), the remote effect was enhanced up to 6-fold. We analyzed the role of nsEP number, amplitude, rotation, and inter-pulse delay, and showed how remote focusing is enhanced when re-created bipolar waveforms exhibit stronger cancellation. Advantages of NG-CANCAN include the exceptional versatility of designing pulse packets and easy remote focusing using an off-the-shelf 4-channel nsEP generator.
MeSH term(s)	Cricetinae ; Animals ; Cell Membrane Permeability ; Cricetulus ; Electricity ; Electroporation/methods ; Electroporation Therapies ; CHO Cells ; Electric Stimulation/methods
Language	English
Publishing date	2023-04-05
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2010650-6
ISSN	1878-562X ; 0302-4598 ; 1567-5394
ISSN (online)	1878-562X
ISSN	0302-4598 ; 1567-5394
DOI	10.1016/j.bioelechem.2023.108437
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Article ; Online: Voltage effects on muscarinic acetylcholine receptor-mediated contractions of airway smooth muscle.

Semenov, Iurii / Brenner, Robert

Physiological reports

2018 Volume 6, Issue 17, Page(s) e13856

Abstract: Studies have shown that the activity of muscarinic receptors and their affinity to agonists are sensitive to membrane potential. It was reported that in airway smooth muscle (ASM) depolarization evoked by high ... ...

Abstract	Studies have shown that the activity of muscarinic receptors and their affinity to agonists are sensitive to membrane potential. It was reported that in airway smooth muscle (ASM) depolarization evoked by high K
MeSH term(s)	Animals ; Calcium Channels/metabolism ; Cells, Cultured ; Membrane Potentials ; Mice ; Mice, Inbred C57BL ; Muscle Contraction ; Myocytes, Smooth Muscle/drug effects ; Myocytes, Smooth Muscle/metabolism ; Myocytes, Smooth Muscle/physiology ; Potassium/pharmacology ; Receptors, Muscarinic/metabolism ; Trachea/cytology
Chemical Substances	Calcium Channels ; Receptors, Muscarinic ; Potassium (RWP5GA015D)
Language	English
Publishing date	2018-09-05
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	2724325-4
ISSN	2051-817X ; 2051-817X
ISSN (online)	2051-817X
ISSN	2051-817X
DOI	10.14814/phy2.13856
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Article ; Online: Pulsed Electric Field Ablation of Esophageal Malignancies and Mitigating Damage to Smooth Muscle: An In Vitro Study.

Gudvangen, Emily / Mangalanathan, Uma / Semenov, Iurii / Kiester, Allen S / Keppler, Mark A / Ibey, Bennett L / Bixler, Joel N / Pakhomov, Andrei G

International journal of molecular sciences

2023 Volume 24, Issue 3

Abstract: Cancer ablation therapies aim to be efficient while minimizing damage to healthy tissues. Nanosecond pulsed electric field (nsPEF) is a promising ablation modality because of its selectivity against certain cell types and reduced neuromuscular effects. ... ...

Abstract	Cancer ablation therapies aim to be efficient while minimizing damage to healthy tissues. Nanosecond pulsed electric field (nsPEF) is a promising ablation modality because of its selectivity against certain cell types and reduced neuromuscular effects. We compared cell killing efficiency by PEF (100 pulses, 200 ns-10 µs duration, 10 Hz) in a panel of human esophageal cells (normal and pre-malignant epithelial and smooth muscle). Normal epithelial cells were less sensitive than the pre-malignant ones to unipolar PEF (15-20% higher LD50,
MeSH term(s)	Humans ; Electricity ; Membrane Potentials ; Electroporation ; Carcinoma ; Muscle, Smooth ; Esophageal Neoplasms/therapy
Language	English
Publishing date	2023-02-02
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms24032854
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Article ; Online: Interference targeting of bipolar nanosecond electric pulses for spatially focused electroporation, electrostimulation, and tissue ablation.

Pakhomov, Andrei G / Gudvangen, Emily / Xiao, Shu / Semenov, Iurii

Bioelectrochemistry (Amsterdam, Netherlands)

2021 Volume 141, Page(s) 107876

Abstract: Stimulation and electroporation by nanosecond electric pulses (nsEP) are distinguished by a phenomenon of bipolar cancellation, which stands for a reduced efficiency of bipolar pulses compared to unipolar ones. When two pairs of stimulating electrodes ... ...

Abstract	Stimulation and electroporation by nanosecond electric pulses (nsEP) are distinguished by a phenomenon of bipolar cancellation, which stands for a reduced efficiency of bipolar pulses compared to unipolar ones. When two pairs of stimulating electrodes are arrayed in a quadrupole, bipolar cancellation inhibits nsEP effects near the electrodes, where the electric field is the strongest. Two properly shaped and synchronized bipolar nsEP overlay into a unipolar pulse towards the center of the electrode array, thus canceling the bipolar cancellation (a "CANCAN effect"). High efficiency of the re-created unipolar nsEP outweighs the weakening of the electric field with distance and focuses nsEP effects to the center. In monolayers of CHO, BPAE, and HEK cells, CANCAN effect achieved by the interference of two bipolar nsEP enhanced electroporation up to tenfold, with a peak at the quadrupole center. Introducing a time interval between bipolar nsEP prevented the formation of a unipolar pulse and eliminated the CANCAN effect. Strong electroporation by CANCAN stimuli killed cells over the entire area encompassed by the electrodes, whereas the time-separated pulses caused ablation only in the strongest electric field near the electrodes. The CANCAN approach is promising for uniform tumor ablation and stimulation targeting away from electrodes.
MeSH term(s)	Animals ; CHO Cells ; Cricetinae ; Cricetulus ; Electric Stimulation/methods ; Electroporation/methods ; Humans
Language	English
Publishing date	2021-06-15
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2010650-6
ISSN	1878-562X ; 0302-4598 ; 1567-5394
ISSN (online)	1878-562X
ISSN	0302-4598 ; 1567-5394
DOI	10.1016/j.bioelechem.2021.107876
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Article ; Online: The role of reactive oxygen species in the immunity induced by nano-pulse stimulation.

Guo, Siqi / Burcus, Niculina I / Scott, Megan / Jing, Yu / Semenov, Iurii

Scientific reports

2021 Volume 11, Issue 1, Page(s) 23745

Abstract: Reactive oxygen species (ROS) are byproducts of tumor cells treated with Nano-Pulse Stimulation (NPS). Recently, ROS have been suggested as a contributing factor in immunogenic cell death and T cell-mediated immunity. This research further investigated ... ...

Abstract	Reactive oxygen species (ROS) are byproducts of tumor cells treated with Nano-Pulse Stimulation (NPS). Recently, ROS have been suggested as a contributing factor in immunogenic cell death and T cell-mediated immunity. This research further investigated the role of NPS induced ROS in antitumor immunity. ROS production in 4T1-luc breast cancer cells was characterized using three detection reagents, namely, Amplex Red, MitoSox Red, and Dihydroethidium. The efficiency of ROS quenching was evaluated in the presence or absence of ROS scavengers and/or antioxidants. The immunogenicity of NPS treated tumor cells was assessed by ex vivo dendritic cell activation, in vivo vaccination assay and in situ vaccination with NPS tumor ablation. We found that NPS treatment enhanced the immunogenicity of 4T1-luc mouse mammary tumor, resulted in a potent in situ vaccination protection and induced long-term T cell immunity. ROS production derived from NPS treated breast cancer cells was an electric pulse dose-dependent phenomenon. Noticeably, the dynamic pattern of hydrogen peroxide production was different from that of superoxide production. Interestingly, regardless of NPS treatment, different ROS scavengers could either block or promote ROS production and stimulate or inhibit tumor cell growth. The activation of dendritic cells was not influenced by blocking ROS generation. The results from in vivo vaccination with NPS treated cancer cells suggests that ROS generation was not a prerequisite for immune protection.
MeSH term(s)	Cell Line, Tumor ; Dendritic Cells/immunology ; Dendritic Cells/metabolism ; Humans ; Immunity ; Nanoparticles ; Neoplasms/immunology ; Neoplasms/metabolism ; Oxidation-Reduction ; Reactive Oxygen Species/metabolism ; T-Lymphocyte Subsets/immunology ; T-Lymphocyte Subsets/metabolism
Chemical Substances	Reactive Oxygen Species
Language	English
Publishing date	2021-12-09
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-021-03342-4
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Article ; Online: Action spectra and mechanisms of (in) efficiency of bipolar electric pulses at electroporation.

Kim, Vitalii / Semenov, Iurii / Kiester, Allen S / Keppler, Mark A / Ibey, Bennett L / Bixler, Joel N / Pakhomov, Andrei G

Bioelectrochemistry (Amsterdam, Netherlands)

2022 Volume 149, Page(s) 108319

Abstract: The reversal of the electric field direction inhibits various biological effects of nanosecond electric pulses (nsEP). This feature, known as "bipolar cancellation," enables interference targeting of nsEP bioeffects remotely from stimulating electrodes, ... ...

Abstract	The reversal of the electric field direction inhibits various biological effects of nanosecond electric pulses (nsEP). This feature, known as "bipolar cancellation," enables interference targeting of nsEP bioeffects remotely from stimulating electrodes, for prospective applications such as precise cancer ablation and non-invasive deep brain stimulation. This study was undertaken to achieve the maximum cancellation of electroporation, by quantifying the impact of the pulse shape, duration, number, and repetition rate across a broad range of electric field strengths. Monolayers of endothelial cells (BPAE) were electroporated in a non-uniform electric field. Cell membrane permeabilization was quantified by YO-PRO-1 (YP) dye uptake and correlated to local electric field strength. For most conditions tested, adding an opposite polarity phase reduced YP uptake by 50-80 %. The strongest cancellation, which reduced YP uptake by 95-97 %, was accomplished by adding a 50 % second phase to 600-ns pulses delivered at a high repetition rate of 833 kHz. Strobe photography of nanosecond kinetics of membrane potential in single CHO cells revealed the temporal summation of polarization by individual unipolar nsEP applied at sub-MHz rate, leading to enhanced electroporation. In contrast, there was no summation for bipolar pulses, and increasing their repetition rate suppressed electroporation. These new findings are discussed in the context of bipolar cancellation mechanisms and remote focusing applications.
MeSH term(s)	Cricetinae ; Animals ; Cricetulus ; Cell Membrane Permeability ; Endothelial Cells ; Electroporation ; CHO Cells
Language	English
Publishing date	2022-11-08
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2010650-6
ISSN	1878-562X ; 0302-4598 ; 1567-5394
ISSN (online)	1878-562X
ISSN	0302-4598 ; 1567-5394
DOI	10.1016/j.bioelechem.2022.108319
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Article ; Online: The role of reactive oxygen species in the immunity induced by nano-pulse stimulation

Siqi Guo / Niculina I. Burcus / Megan Scott / Yu Jing / Iurii Semenov

Scientific Reports, Vol 11, Iss 1, Pp 1-

2021 Volume 13

Abstract: Abstract Reactive oxygen species (ROS) are byproducts of tumor cells treated with Nano-Pulse Stimulation (NPS). Recently, ROS have been suggested as a contributing factor in immunogenic cell death and T cell-mediated immunity. This research further ... ...

Abstract	Abstract Reactive oxygen species (ROS) are byproducts of tumor cells treated with Nano-Pulse Stimulation (NPS). Recently, ROS have been suggested as a contributing factor in immunogenic cell death and T cell-mediated immunity. This research further investigated the role of NPS induced ROS in antitumor immunity. ROS production in 4T1-luc breast cancer cells was characterized using three detection reagents, namely, Amplex Red, MitoSox Red, and Dihydroethidium. The efficiency of ROS quenching was evaluated in the presence or absence of ROS scavengers and/or antioxidants. The immunogenicity of NPS treated tumor cells was assessed by ex vivo dendritic cell activation, in vivo vaccination assay and in situ vaccination with NPS tumor ablation. We found that NPS treatment enhanced the immunogenicity of 4T1-luc mouse mammary tumor, resulted in a potent in situ vaccination protection and induced long-term T cell immunity. ROS production derived from NPS treated breast cancer cells was an electric pulse dose-dependent phenomenon. Noticeably, the dynamic pattern of hydrogen peroxide production was different from that of superoxide production. Interestingly, regardless of NPS treatment, different ROS scavengers could either block or promote ROS production and stimulate or inhibit tumor cell growth. The activation of dendritic cells was not influenced by blocking ROS generation. The results from in vivo vaccination with NPS treated cancer cells suggests that ROS generation was not a prerequisite for immune protection.
Keywords	Medicine ; R ; Science ; Q
Language	English
Publishing date	2021-12-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Control of the Electroporation Efficiency of Nanosecond Pulses by Swinging the Electric Field Vector Direction.

Kim, Vitalii / Semenov, Iurii / Kiester, Allen S / Keppler, Mark A / Ibey, Bennett L / Bixler, Joel N / Colunga Biancatelli, Ruben M L / Pakhomov, Andrei G

International journal of molecular sciences

2023 Volume 24, Issue 13

Abstract: Reversing the pulse polarity, i.e., changing the electric field direction by 180°, inhibits electroporation and electrostimulation by nanosecond electric pulses (nsEPs). This feature, known as "bipolar cancellation," enables selective remote targeting ... ...

Abstract	Reversing the pulse polarity, i.e., changing the electric field direction by 180°, inhibits electroporation and electrostimulation by nanosecond electric pulses (nsEPs). This feature, known as "bipolar cancellation," enables selective remote targeting with nsEPs and reduces the neuromuscular side effects of ablation therapies. We analyzed the biophysical mechanisms and measured how cancellation weakens and is replaced by facilitation when nsEPs are applied from different directions at angles from 0 to 180°. Monolayers of endothelial cells were electroporated by a train of five pulses (600 ns) or five paired pulses (600 + 600 ns) applied at 1 Hz or 833 kHz. Reversing the electric field in the pairs (180° direction change) caused 2-fold (1 Hz) or 20-fold (833 kHz) weaker electroporation than the train of single nsEPs. Reducing the angle between pulse directions in the pairs weakened cancellation and replaced it with facilitation at angles <160° (1 Hz) and <130° (833 kHz). Facilitation plateaued at about three-fold stronger electroporation compared to single pulses at 90-100° angle for both nsEP frequencies. The profound dependence of the efficiency on the angle enables novel protocols for highly selective focal electroporation at one electrode in a three-electrode array while avoiding effects at the other electrodes. Nanosecond-resolution imaging of cell membrane potential was used to link the selectivity to charging kinetics by co- and counter-directional nsEPs.
MeSH term(s)	Cell Membrane/metabolism ; Cell Membrane Permeability ; Endothelial Cells ; Electroporation/methods ; Electroporation Therapies
Language	English
Publishing date	2023-06-30
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms241310921
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Article ; Online: Excitation of murine cardiac myocytes by nanosecond pulsed electric field.

Azarov, Jan E / Semenov, Iurii / Casciola, Maura / Pakhomov, Andrei G

Journal of cardiovascular electrophysiology

2019 Volume 30, Issue 3, Page(s) 392–401

Abstract: Introduction: Opening of voltage-gated sodium channels takes tens to hundreds of microseconds, and mechanisms of their opening by nanosecond pulsed electric field (nsPEF) stimuli remain elusive. This study was aimed at uncovering the mechanisms of how ... ...

Abstract	Introduction: Opening of voltage-gated sodium channels takes tens to hundreds of microseconds, and mechanisms of their opening by nanosecond pulsed electric field (nsPEF) stimuli remain elusive. This study was aimed at uncovering the mechanisms of how nsPEF elicits action potentials (APs) in cardiomyocytes. Methods and results: Fluorescent imaging of optical APs (FluoVolt) and Ca Conclusions: The study established that nsPEF stimulation caused calcium entry into cardiac myocytes (including routes other than voltage-gated calcium channels) and SSD. Tetrodotoxin-sensitive APs were mediated by SSD, whose amplitude depended on the calcium entry. Plasma membrane electroporation was the most likely primary mechanism of SSD with additional contribution from l-type calcium and sodium-calcium exchanger currents.
MeSH term(s)	Action Potentials ; Animals ; Calcium/metabolism ; Calcium Channels, L-Type/metabolism ; Calcium Signaling ; Electric Stimulation ; Mice, Inbred DBA ; Myocytes, Cardiac/metabolism ; Sodium/metabolism ; Sodium-Calcium Exchanger/metabolism ; Time Factors
Chemical Substances	Calcium Channels, L-Type ; Sodium-Calcium Exchanger ; Sodium (9NEZ333N27) ; Calcium (SY7Q814VUP)
Language	English
Publishing date	2019-01-17
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	1025989-2
ISSN	1540-8167 ; 1045-3873
ISSN (online)	1540-8167
ISSN	1045-3873
DOI	10.1111/jce.13834
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