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  1. Article ; Online: In Reply - Safety of Convalescent Plasma Transfusion.

    Juskewitch, Justin E / Stubbs, James R / Gandhi, Manish J

    Mayo Clinic proceedings

    2021  Volume 96, Issue 5, Page(s) 1369

    MeSH term(s) Blood Component Transfusion/adverse effects ; Blood Transfusion ; COVID-19 ; Humans ; Immunization, Passive ; Plasma
    Language English
    Publishing date 2021-05-06
    Publishing country England
    Document type Letter ; Comment
    ZDB-ID 124027-4
    ISSN 1942-5546 ; 0025-6196
    ISSN (online) 1942-5546
    ISSN 0025-6196
    DOI 10.1016/j.mayocp.2021.03.026
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Emergent thrombocytapheresis for essential thrombocythemia following splenectomy.

    Juskewitch, Justin E / Welch, Amy L / Welch, Brian M / Kreuter, Justin D

    Transfusion

    2020  Volume 60, Issue 6, Page(s) 1133–1134

    MeSH term(s) Adult ; Amino Acid Substitution ; Humans ; Janus Kinase 2/genetics ; Male ; Mutation, Missense ; Plateletpheresis ; Splenectomy ; Thrombocythemia, Essential/enzymology ; Thrombocythemia, Essential/genetics ; Thrombocythemia, Essential/therapy
    Chemical Substances JAK2 protein, human (EC 2.7.10.2) ; Janus Kinase 2 (EC 2.7.10.2)
    Language English
    Publishing date 2020-03-06
    Publishing country United States
    Document type Case Reports ; Journal Article
    ZDB-ID 208417-x
    ISSN 1537-2995 ; 0041-1132
    ISSN (online) 1537-2995
    ISSN 0041-1132
    DOI 10.1111/trf.15746
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  3. Article ; Online: Elevated Rate of HLA Antibodies in Male COVID-19 Convalescent Plasma Donors: A Risk Factor for Transfusion-Related Acute Lung Injury.

    Juskewitch, Justin E / Stubbs, James R / Gandhi, Manish J

    Mayo Clinic proceedings

    2020  Volume 96, Issue 2, Page(s) 500–502

    MeSH term(s) Adult ; Antibodies/blood ; Blood Donors ; COVID-19/blood ; COVID-19/therapy ; Convalescence ; HLA Antigens/immunology ; Humans ; Male ; Middle Aged ; Plasma ; Risk Factors ; Transfusion-Related Acute Lung Injury/epidemiology ; Transfusion-Related Acute Lung Injury/etiology
    Chemical Substances Antibodies ; HLA Antigens
    Language English
    Publishing date 2020-11-23
    Publishing country England
    Document type Letter
    ZDB-ID 124027-4
    ISSN 1942-5546 ; 0025-6196
    ISSN (online) 1942-5546
    ISSN 0025-6196
    DOI 10.1016/j.mayocp.2020.11.007
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Development and performance characteristics of Platelet Virtual Crossmatch (PLT VXM), a software application for the evaluation and management of platelet transfusion-refractory patients.

    Juskewitch, Justin E / Gandhi, Manish J / Kreuter, Justin D / Norgan, Andrew P

    Transfusion

    2020  Volume 60, Issue 10, Page(s) 2284–2293

    Abstract: Background: Platelet (PLT) transfusion refractoriness increases bleeding complications, hospital stays, and PLT inventory usage. Immune-mediated refractoriness can be evaluated for using a physical PLT crossmatch with ABO-compatible inventory and, if ... ...

    Abstract Background: Platelet (PLT) transfusion refractoriness increases bleeding complications, hospital stays, and PLT inventory usage. Immune-mediated refractoriness can be evaluated for using a physical PLT crossmatch with ABO-compatible inventory and, if positive, managed with HLA-compatible PLT inventory and donors. Manual completion of these complex tasks can be time-consuming and potentially error-prone. This study was conducted to determine if a Web-based software application could improve process efficiency and accuracy.
    Study design and methods: Workflow analysis was performed to identify process, data, and analytic requirements for a software application for three PLT transfusion-refractoriness associated tasks: (a) physical PLT crossmatch inventory selection, (b) HLA-compatible inventory selection, and (c) HLA-compatible donor selection. After software application development, a comparison study was performed over 10 consecutive days, with each task performed manually and with the software application (Platelet Virtual Crossmatch [PLT VXM]) for a different unique immune-mediated PLT transfusion-refractory recipient. Task completion time, number of incompatible units/donors presented, and number of documentation errors were compared.
    Results: PLT VXM is a Web-based software application developed using R and the Shiny Web application framework. PLT VXM significantly reduced median task completion times by 4.5 (49%), 11.2 (79%), and 59.1 minutes (94%), respectively. PLT VXM did not present any incompatible PLT units or donors for user consideration. PLT VXM also had a lower number of documentation errors than the manual process, and none of these documentation errors were software generated.
    Conclusion: Computer-aided evaluation and management of immune-mediated PLT transfusion-refractory recipients can significantly improve workflow and reduce manual errors in this complex process.
    MeSH term(s) ABO Blood-Group System/blood ; Adult ; Blood Grouping and Crossmatching ; Blood Platelets ; Donor Selection ; HLA Antigens ; Humans ; Internet ; Middle Aged ; Platelet Transfusion ; Software
    Chemical Substances ABO Blood-Group System ; HLA Antigens
    Language English
    Publishing date 2020-08-21
    Publishing country United States
    Document type Evaluation Study ; Journal Article
    ZDB-ID 208417-x
    ISSN 1537-2995 ; 0041-1132
    ISSN (online) 1537-2995
    ISSN 0041-1132
    DOI 10.1111/trf.16025
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: How do we leverage data through replication and warehousing to meet blood collection and transfusion service needs.

    Juskewitch, Justin E / Briggs, Lynne / Khan, Jenna / Mathias, Patrick C / Coyle, Terri S / Courson, Vicki L / Hansen, James T / Madde, Nageswar / O'Leary, Mandy F / Tsang, Hamilton C

    Transfusion

    2024  

    Abstract: Background: With the widespread adoption of Blood Establishment Computer Systems and other Blood Collection and Transfusion Service (BCTS) clinical information systems (CIS), electronic blood donor, product, and patient data are now routinely required ... ...

    Abstract Background: With the widespread adoption of Blood Establishment Computer Systems and other Blood Collection and Transfusion Service (BCTS) clinical information systems (CIS), electronic blood donor, product, and patient data are now routinely required for clinical, regulatory, operational, and quality needs. That data are often not readily accessible for such secondary use within CIS databases, particularly for applications with significant data availability requirements such as machine learning and artificial intelligence. Data replication provides one avenue by which CIS data can be made more readily available.
    Study design and methods: Members of the AABB's Information Systems Committee along with institutional information technology colleagues provided a multi-institutional viewpoint on data replication through the lens of BCTS specific use cases. Case studies of informatics offerings leveraging such technologies were also elicited.
    Results: Six distinct use cases describe the potential role of data replication including the creation of data warehouses for frontline laboratory staff. Specific BCTS examples for each use case are presented to highlight the value of data replication, including visualization of critical inventory (O red blood cells, HLA-compatible platelets) and utilization analytics for patient blood management. Two case studies describe the approach to implement such technologies to (1) optimize staffing via laboratory workload reporting and (2) improve access to blood via antigen-negative blood product location services.
    Discussion: Data replication and warehousing can empower BCTS analytic offerings not otherwise natively available through one's CIS to improve patient care and laboratory operations.
    Language English
    Publishing date 2024-04-22
    Publishing country United States
    Document type Case Reports
    ZDB-ID 208417-x
    ISSN 1537-2995 ; 0041-1132
    ISSN (online) 1537-2995
    ISSN 0041-1132
    DOI 10.1111/trf.17845
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Prozone rates in the solid-phase platelet crossmatch assay and correlation with class I HLA antibody levels.

    Juskewitch, Justin E / Zuccarelli, Micah D / Clymer, Kristie K / Wakefield, Laurie L / Kreuter, Justin D / Gandhi, Manish J

    Transfusion

    2021  Volume 61, Issue 11, Page(s) 3236–3246

    Abstract: Background: Solid-phase platelet crossmatch (PXM) testing is used to help manage patients with platelet transfusion-refractoriness. Recently, we published the first report of false-negative PXM results from prozone effect that was mitigated using sample ...

    Abstract Background: Solid-phase platelet crossmatch (PXM) testing is used to help manage patients with platelet transfusion-refractoriness. Recently, we published the first report of false-negative PXM results from prozone effect that was mitigated using sample dilution. This study aimed to describe the prevalence of PXM prozone effect and the levels of class I HLA antibodies (HLA-Abs) associated with positive PXM results and with false-negative PXM results from prozone effect.
    Study design and methods: A cross-sectional study of patients undergoing PXM testing from July 2019 through December 2020 was performed. All PXM tests were run simultaneously using undiluted and 1:4 diluted patient plasma. Prozone effect was defined as a negative PXM result using undiluted patient plasma but a positive PXM result using 1:4 diluted patient plasma.
    Results: Among 59 patients, 830 individual ABO-compatible PXM results yielded an overall positivity rate of 25.8% (214/830) and a false-negative rate from prozone effect of 4.7% (10/214). Among the 28 patients with class I HLA-Ab testing and no other anti-platelet antibodies, maximum HLA-Ab mean fluorescence intensity (MFI) was significantly associated with a positive PXM result (p < .0001; AUC approx. 0.9) and categorized into negative (<3700), indeterminate (3700-10300), and positive (>10300) maximum HLA-Ab MFI zones. Maximum HLA-Ab MFI, however, was not associated with prozone effect (p = .17; AUC approx. 0.6).
    Discussion: While there is a strong predictive association between class I HLA-Ab levels and positive PXM results, PXM prozone effect is a common occurrence not associated with class I HLA-Ab levels, so additional testing with diluted samples should be considered.
    MeSH term(s) Antibodies ; Cross-Sectional Studies ; HLA Antigens ; Histocompatibility Testing/methods ; Humans ; Isoantibodies
    Chemical Substances Antibodies ; HLA Antigens ; Isoantibodies
    Language English
    Publishing date 2021-09-15
    Publishing country United States
    Document type Journal Article
    ZDB-ID 208417-x
    ISSN 1537-2995 ; 0041-1132
    ISSN (online) 1537-2995
    ISSN 0041-1132
    DOI 10.1111/trf.16660
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: HLA Antibody Rates Are Not Increased in a Regional Group of Male COVID-19 Convalescent Plasma Donors.

    Juskewitch, Justin E / Senefeld, Jonathon W / Johnson, Patrick W / Mills, John R / Joyner, Michael J / Gandhi, Manish J

    Mayo Clinic proceedings

    2021  Volume 96, Issue 10, Page(s) 2727–2728

    MeSH term(s) Adult ; Aged ; Aged, 80 and over ; Antibodies, Viral/analysis ; Antibodies, Viral/blood ; Blood Donors ; COVID-19/immunology ; COVID-19/virology ; HLA Antigens/immunology ; Humans ; Isoantibodies/immunology ; Male ; Middle Aged ; SARS-CoV-2/immunology
    Chemical Substances Antibodies, Viral ; HLA Antigens ; Isoantibodies
    Language English
    Publishing date 2021-08-20
    Publishing country England
    Document type Letter ; Research Support, Non-U.S. Gov't
    ZDB-ID 124027-4
    ISSN 1942-5546 ; 0025-6196
    ISSN (online) 1942-5546
    ISSN 0025-6196
    DOI 10.1016/j.mayocp.2021.08.004
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Vaccine-boosted convalescent plasma therapy for patients with immunosuppression and COVID-19.

    Ripoll, Juan G / Gorman, Ellen K / Juskewitch, Justin E / Razonable, Raymund R / Ganesh, Ravindra / Hurt, Ryan T / Theel, Elitza S / Stubbs, James R / Winters, Jeffrey L / Parikh, Sameer A / Kay, Neil E / Joyner, Michael J / Senefeld, Jonathon W

    Blood advances

    2022  Volume 6, Issue 23, Page(s) 5951–5955

    MeSH term(s) Humans ; COVID-19/therapy ; Immunization, Passive ; Vaccines ; Immunosuppression Therapy ; COVID-19 Serotherapy
    Chemical Substances Vaccines
    Language English
    Publishing date 2022-09-27
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2915908-8
    ISSN 2473-9537 ; 2473-9529
    ISSN (online) 2473-9537
    ISSN 2473-9529
    DOI 10.1182/bloodadvances.2022008932
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  9. Article: To Give or Not to Give: RhD Immunoglobulin for an

    Juskewitch, Justin E / Tauscher, Craig D / Moldenhauer, Sheila K / Schieber, Jennifer E / Jacob, Eapen K / DiGuardo, Margaret A

    Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie

    2021  Volume 48, Issue 4, Page(s) 244–249

    Abstract: ... an : Case presentation: Ms. S is B positive with a reported history of anti-D, anti-C, and anti-E ...

    Abstract Introduction: Patients with sickle cell disease (SCD) have repeated episodes of red blood cell (RBC) sickling and microvascular occlusion that manifest as pain crises, acute chest syndrome, and chronic hemolysis. These clinical sequelae usually increase during pregnancy. Given the racial distribution of SCD, patients with SCD are also more likely to have rarer RBC antigen genotypes than RBC donor populations. We present the management and clinical outcome of a 21-year-old pregnant woman with SCD and an
    Case presentation: Ms. S is B positive with a reported history of anti-D, anti-C, and anti-E alloantibodies (anti-G testing unknown). Genetic testing revealed both an
    Discussion/conclusion: Ms. S represents a rare
    Language English
    Publishing date 2021-02-25
    Publishing country Switzerland
    Document type Case Reports
    ZDB-ID 2100848-6
    ISSN 1660-3818 ; 1660-3796
    ISSN (online) 1660-3818
    ISSN 1660-3796
    DOI 10.1159/000512644
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  10. Article ; Online: The use of cytapheresis in the treatment of infectious diseases.

    Norgan, Andrew P / Juskewitch, Justin E / Pritt, Bobbi S / Winters, Jeffrey L

    Journal of clinical apheresis

    2018  Volume 33, Issue 4, Page(s) 529–537

    Abstract: Cytapheresis (removal of cellular blood components) has been employed for treatment of infectious diseases since the 1960s. Techniques have included thrombocytapheresis (buffy coat apheresis) for loiasis, erythrocytapheresis for malaria and babesiosis, ... ...

    Abstract Cytapheresis (removal of cellular blood components) has been employed for treatment of infectious diseases since the 1960s. Techniques have included thrombocytapheresis (buffy coat apheresis) for loiasis, erythrocytapheresis for malaria and babesiosis, and leukocytapheresis for pertussis-associated lymphocytosis. Published data on these applications is largely limited to case level data and small observational studies; as such, recommendations for or against the use of cytapheresis in the treatment of infections have been extrapolated from these limited (and at times flawed) data sets. Consequently, utilization of cytapheresis in many instances is not uniform between institutions, and typically occurs at the discretion of treating medical teams. This review revisits the existing literature on the use of cytapheresis in the treatment of four infections (loasis, malaria, babesiosis, and pertussis) and examines the rationale underlying current treatment recommendations concerning its use.
    MeSH term(s) Babesiosis/therapy ; Communicable Diseases/therapy ; Cytapheresis/methods ; Humans ; Loiasis/therapy ; Malaria/therapy ; Whooping Cough/therapy
    Language English
    Publishing date 2018-02-28
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 604912-6
    ISSN 1098-1101 ; 0733-2459
    ISSN (online) 1098-1101
    ISSN 0733-2459
    DOI 10.1002/jca.21620
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